Spg028575 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg028575 vs. NCBI nr
Match: XP_023516881.1 (cytochrome P450 714A1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 837.8 bits (2163), Expect = 5.2e-239
Identity = 423/532 (79.51%), Postives = 470/532 (88.35%), Query Frame = 0

Query: 6   LDGLVSIFPAGVAMAVVAVLVHFYYGVWRRTAEVRRKLGAQGVTGPPPSSALFGNLPEMK 65
           + GL S+  A VA+AVVAV+VHFYYGVWRRTAE+RRKL AQGVTGPPP S L+GNLPEM+
Sbjct: 1   MGGLGSVLFAAVAVAVVAVVVHFYYGVWRRTAEIRRKLRAQGVTGPPP-SLLYGNLPEMQ 60

Query: 66  EIELQ--AAAMASPPHHPSPTVAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLYANDV 125
           EI+L+  AAAMASPPHH S  VAHDY STLFPYFVKWRKQYGPLYTYSTGTRQHLYAN V
Sbjct: 61  EIQLEAAAAAMASPPHHASVIVAHDYTSTLFPYFVKWRKQYGPLYTYSTGTRQHLYANHV 120

Query: 126 DLVKDLSLSKNPDLDKPSYIFKKFAPLLGYGVILSNGADWTLQHKIIAPEFFMDRVRGMA 185
           DLVKDLSL+ +  L KPSY+ KK AP+LG+ V+ +NG  W LQ KIIAPEFFMDRVR MA
Sbjct: 121 DLVKDLSLTNHLHLGKPSYVTKKLAPILGHSVVRANGPVWALQRKIIAPEFFMDRVRVMA 180

Query: 186 ALMVEAAAPMLEKWEGRIGEEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGKQIFS 245
           ALM EAA+ ML+KWEGRIGE  +GA AEI+V+E LR FSADVISRACFGSSYEKGK+IFS
Sbjct: 181 ALMTEAASAMLQKWEGRIGER-DGATAEIDVDEALREFSADVISRACFGSSYEKGKRIFS 240

Query: 246 KLRDLQKLISDGNPLF-RSSFSDRFFQPSKHRRIKKLEKEIESLIWETVQQRQEECSKTS 305
           KLR LQKL+S+GN LF   S SDR FQ  KHRRIKKLEKEIESLIWETVQQRQ ECSKT 
Sbjct: 241 KLRVLQKLLSEGNFLFGYVSLSDRLFQQPKHRRIKKLEKEIESLIWETVQQRQRECSKT- 300

Query: 306 SSEKDLLQVLMEGAMNDPTIGA-CNESCKKFIVDNCKTMYFAGHESSAATATWALMLLAL 365
           SS+KDLLQ++ME AMNDPT+GA CNES KKFIVDNCK++YFAGHES+A  ATW+LMLLAL
Sbjct: 301 SSDKDLLQLIMEAAMNDPTVGANCNESPKKFIVDNCKSIYFAGHESTAVAATWSLMLLAL 360

Query: 366 HPEWQDRIRSEFTQACPDGRLDTTAISQLKSLSMVVQETLRLYPPGAYVARETFAETRLG 425
           HPEWQDRIRSEFTQACPDGR++ T ISQLKS+SMVVQETLRLYPP A+VARETF+ETRLG
Sbjct: 361 HPEWQDRIRSEFTQACPDGRVNMTTISQLKSVSMVVQETLRLYPPAAFVARETFSETRLG 420

Query: 426 NVVVPKGVCLWTLIPMLHTEVEIWGEDANEFKPERFANGIAKACKFPQAYLPFGAGHRTC 485
           NVV+PKGV LWTLIP LH EVEIWGE+ANEFKPERF NGIAKACKFPQAY+PFGAG R C
Sbjct: 421 NVVIPKGVYLWTLIPTLHREVEIWGENANEFKPERFVNGIAKACKFPQAYVPFGAGPRLC 480

Query: 486 LGKNFALVQLKIIISLVVSKFRFSLSPEYRHSPSYRIIVEPDDGVKIVFQRL 534
           LGKNFALVQLKIIISL+V KFRFSLSPEYRHSPSYR+IVEP +G+KI+F+R+
Sbjct: 481 LGKNFALVQLKIIISLIVFKFRFSLSPEYRHSPSYRMIVEPANGMKIIFKRV 529

BLAST of Spg028575 vs. NCBI nr
Match: KAG6599934.1 (Cytochrome P450 714A1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 834.3 bits (2154), Expect = 5.8e-238
Identity = 422/533 (79.17%), Postives = 469/533 (87.99%), Query Frame = 0

Query: 6   LDGLVSIFPAGVAMAVVAVLVHFYYGVWRRTAEVRRKLGAQGVTGPPPSSALFGNLPEMK 65
           + GL S+  A VA+AVVAV+VHFYYGVWRRTAE+RRKL AQGVTGPPP S L+GNLPEM+
Sbjct: 1   MGGLGSVLFAAVAVAVVAVVVHFYYGVWRRTAEIRRKLRAQGVTGPPP-SLLYGNLPEMQ 60

Query: 66  EIELQ---AAAMASPPHHPSPTVAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLYAND 125
           EI+L+   AAAMASPPHH S  VAHDY STLFPYFVKWRKQYGPLYTYSTGTRQHLYAN 
Sbjct: 61  EIQLEAAAAAAMASPPHHASVIVAHDYTSTLFPYFVKWRKQYGPLYTYSTGTRQHLYANH 120

Query: 126 VDLVKDLSLSKNPDLDKPSYIFKKFAPLLGYGVILSNGADWTLQHKIIAPEFFMDRVRGM 185
           VDLVKDLSL+ +  L KPSY+ KK AP+LG+ ++ +NG  W LQ KIIAPEFFMDRVR M
Sbjct: 121 VDLVKDLSLTNHLHLGKPSYVTKKLAPILGHSIVRANGPVWALQRKIIAPEFFMDRVRVM 180

Query: 186 AALMVEAAAPMLEKWEGRIGEEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGKQIF 245
           AALM EAAA ML+KWE RIG EG+GA AEI+V+E LR FSADVISRACFGSSYEKGK+IF
Sbjct: 181 AALMTEAAAAMLQKWEERIG-EGDGATAEIDVDEALREFSADVISRACFGSSYEKGKRIF 240

Query: 246 SKLRDLQKLISDGNPLF-RSSFSDRFFQPSKHRRIKKLEKEIESLIWETVQQRQEECSKT 305
           SKLR LQKL+S+GN LF   S SDR FQ SKHR IKKLEKEIESLIWETVQQRQ+ECSKT
Sbjct: 241 SKLRVLQKLLSEGNFLFGYVSLSDRLFQQSKHRTIKKLEKEIESLIWETVQQRQKECSKT 300

Query: 306 SSSEKDLLQVLMEGAMNDPTIGA-CNESCKKFIVDNCKTMYFAGHESSAATATWALMLLA 365
            SS+KDLLQ++ME AMNDPT+GA CNES KKFIVDNCK++YFAGHES+A  ATW+LMLLA
Sbjct: 301 -SSDKDLLQLIMEAAMNDPTVGANCNESPKKFIVDNCKSIYFAGHESTAVAATWSLMLLA 360

Query: 366 LHPEWQDRIRSEFTQACPDGRLDTTAISQLKSLSMVVQETLRLYPPGAYVARETFAETRL 425
           LHPEWQDRIRSEFTQACPDGR++ T ISQLKS SMVVQETLRLYPP A+VARETF+ETRL
Sbjct: 361 LHPEWQDRIRSEFTQACPDGRVNMTTISQLKSASMVVQETLRLYPPAAFVARETFSETRL 420

Query: 426 GNVVVPKGVCLWTLIPMLHTEVEIWGEDANEFKPERFANGIAKACKFPQAYLPFGAGHRT 485
           GNVV+PKGV LWTLIP LH EVEIWGE+ANEFKPERF NGIAKACKFPQ Y+PFGAG R 
Sbjct: 421 GNVVIPKGVYLWTLIPTLHREVEIWGENANEFKPERFVNGIAKACKFPQTYVPFGAGPRL 480

Query: 486 CLGKNFALVQLKIIISLVVSKFRFSLSPEYRHSPSYRIIVEPDDGVKIVFQRL 534
           CLGKNFALVQLKIIISL+VSKFRFSLS EYRHSPSYR+IVEP +G+KI+F+R+
Sbjct: 481 CLGKNFALVQLKIIISLIVSKFRFSLSSEYRHSPSYRMIVEPANGMKIIFKRV 530

BLAST of Spg028575 vs. NCBI nr
Match: XP_022995469.1 (cytochrome P450 714A1-like [Cucurbita maxima])

HSP 1 Score: 829.3 bits (2141), Expect = 1.9e-236
Identity = 419/531 (78.91%), Postives = 467/531 (87.95%), Query Frame = 0

Query: 6   LDGLVSIFPAGVAMAVVAVLVHFYYGVWRRTAEVRRKLGAQGVTGPPPSSALFGNLPEMK 65
           + GL S+  A VA+AVVAV+VHFYYGVWRRTAE+RRKL AQGVTGPPP S L+GNL EM+
Sbjct: 1   MGGLGSVLFAAVAVAVVAVVVHFYYGVWRRTAEIRRKLRAQGVTGPPP-SLLYGNLQEMQ 60

Query: 66  EIELQ-AAAMASPPHHPSPTVAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLYANDVD 125
           EI+L+ AAAM SPPHH S  VAHDY STLFPYFVKWRKQYGPLYTYSTGTRQHLYAN VD
Sbjct: 61  EIQLEAAAAMVSPPHHASVIVAHDYTSTLFPYFVKWRKQYGPLYTYSTGTRQHLYANHVD 120

Query: 126 LVKDLSLSKNPDLDKPSYIFKKFAPLLGYGVILSNGADWTLQHKIIAPEFFMDRVRGMAA 185
           LVKDLSL+    L KPSY+ KK AP+LG+ ++ +NG  W LQ KIIAPEFFMD+VR MA 
Sbjct: 121 LVKDLSLTNYLHLGKPSYVTKKLAPILGHSIVRANGPVWALQRKIIAPEFFMDQVRVMAT 180

Query: 186 LMVEAAAPMLEKWEGRIGEEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGKQIFSK 245
           LM EAAA ML+KWEGRIG EGEGA AEI+V+E LR FSADVISRACFGSSYEKGK+IFSK
Sbjct: 181 LMTEAAAAMLQKWEGRIG-EGEGATAEIDVDEDLREFSADVISRACFGSSYEKGKRIFSK 240

Query: 246 LRDLQKLISDGNPLF-RSSFSDRFFQPSKHRRIKKLEKEIESLIWETVQQRQEECSKTSS 305
           LR LQKL+S+GN LF   S SDR FQ SKHRRIKKLEKEIESLIWETVQQRQ+ECSKT S
Sbjct: 241 LRVLQKLLSEGNFLFGYVSLSDRLFQQSKHRRIKKLEKEIESLIWETVQQRQKECSKT-S 300

Query: 306 SEKDLLQVLMEGAMNDPTIGA-CNESCKKFIVDNCKTMYFAGHESSAATATWALMLLALH 365
           S+KDLLQ++ME AMNDPT+ A CNES KKFIVDNCK++YFAGHES+A  ATW+LMLLALH
Sbjct: 301 SDKDLLQLIMEAAMNDPTVAANCNESPKKFIVDNCKSIYFAGHESTAVAATWSLMLLALH 360

Query: 366 PEWQDRIRSEFTQACPDGRLDTTAISQLKSLSMVVQETLRLYPPGAYVARETFAETRLGN 425
           PEWQDRIRSEF QAC DGR++ T ISQLKS+SMVVQETLRLYPP A+VARETF+ETRLGN
Sbjct: 361 PEWQDRIRSEFGQACLDGRVNMTTISQLKSVSMVVQETLRLYPPAAFVARETFSETRLGN 420

Query: 426 VVVPKGVCLWTLIPMLHTEVEIWGEDANEFKPERFANGIAKACKFPQAYLPFGAGHRTCL 485
           VV+PKG+ LWTLIP LH EVEIWGE+ANEFKPERF NGIAKACKFPQAYLPFGAG R CL
Sbjct: 421 VVIPKGIYLWTLIPTLHREVEIWGENANEFKPERFVNGIAKACKFPQAYLPFGAGPRLCL 480

Query: 486 GKNFALVQLKIIISLVVSKFRFSLSPEYRHSPSYRIIVEPDDGVKIVFQRL 534
           GKNFALVQLKI+ISL+VSKFRFSLSPEYRHSPSYR+IVEP +G+KI+F+R+
Sbjct: 481 GKNFALVQLKIVISLIVSKFRFSLSPEYRHSPSYRMIVEPANGMKIIFKRV 528

BLAST of Spg028575 vs. NCBI nr
Match: XP_022943066.1 (cytochrome P450 714A1-like isoform X1 [Cucurbita moschata] >XP_022943067.1 cytochrome P450 714A1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 828.6 bits (2139), Expect = 3.2e-236
Identity = 418/532 (78.57%), Postives = 469/532 (88.16%), Query Frame = 0

Query: 6   LDGLVSIFPAGVAMAVVAVLVHFYYGVWRRTAEVRRKLGAQGVTGPPPSSALFGNLPEMK 65
           + GL S+    VA+AVVAV+VHFYYGVWRRTAE+RRKL AQGVTGP P S L+GNLPEM+
Sbjct: 1   MGGLGSVLFTAVAVAVVAVVVHFYYGVWRRTAEIRRKLRAQGVTGPSP-SLLYGNLPEMQ 60

Query: 66  EIELQ--AAAMASPPHHPSPTVAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLYANDV 125
           EI+L+  AAAMASPPHH S  VAHDY STLFPYFVKWRKQYGPLYTYSTGTRQHLYAN V
Sbjct: 61  EIQLEAAAAAMASPPHHASVIVAHDYTSTLFPYFVKWRKQYGPLYTYSTGTRQHLYANHV 120

Query: 126 DLVKDLSLSKNPDLDKPSYIFKKFAPLLGYGVILSNGADWTLQHKIIAPEFFMDRVRGMA 185
           DLVKDLSL+ +  L KPSY+ KK AP+LG+ ++ +NG  W LQ KIIAPEFFMDRVR MA
Sbjct: 121 DLVKDLSLTNHLHLGKPSYVTKKLAPILGHSIVRANGPVWALQRKIIAPEFFMDRVRVMA 180

Query: 186 ALMVEAAAPMLEKWEGRIGEEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGKQIFS 245
           ALM EAAA ML+KWE RI +EG+GA AEI+V+E LR FSADVISRACFGSSYEKGK+IFS
Sbjct: 181 ALMTEAAAAMLQKWEERI-DEGDGATAEIDVDEALREFSADVISRACFGSSYEKGKRIFS 240

Query: 246 KLRDLQKLISDGNPLF-RSSFSDRFFQPSKHRRIKKLEKEIESLIWETVQQRQEECSKTS 305
           KLR LQKL+S+GN LF   S SDR FQ SKHR IKKLEKEIESLIWETVQQRQ+ECSKT 
Sbjct: 241 KLRVLQKLLSEGNFLFGYVSLSDRLFQQSKHRTIKKLEKEIESLIWETVQQRQKECSKT- 300

Query: 306 SSEKDLLQVLMEGAMNDPTIGA-CNESCKKFIVDNCKTMYFAGHESSAATATWALMLLAL 365
           SS+KDLLQ++ME AMNDPT+G+ CNES KKFIVDNCK++YFAGHES+A  ATW+LMLLAL
Sbjct: 301 SSDKDLLQLIMEAAMNDPTVGSNCNESPKKFIVDNCKSIYFAGHESTAVAATWSLMLLAL 360

Query: 366 HPEWQDRIRSEFTQACPDGRLDTTAISQLKSLSMVVQETLRLYPPGAYVARETFAETRLG 425
           HPEWQDRIRSEFTQACPDGR++ TAISQLKS SMVVQETLRLYPP A+VARETF+ETRLG
Sbjct: 361 HPEWQDRIRSEFTQACPDGRVNMTAISQLKSASMVVQETLRLYPPAAFVARETFSETRLG 420

Query: 426 NVVVPKGVCLWTLIPMLHTEVEIWGEDANEFKPERFANGIAKACKFPQAYLPFGAGHRTC 485
           NVV+PKGV LWTLIP LH EVEIWGE+AN+FKPERF NGIAKACKFPQAY+PFGAG R C
Sbjct: 421 NVVIPKGVYLWTLIPTLHREVEIWGENANKFKPERFVNGIAKACKFPQAYVPFGAGPRLC 480

Query: 486 LGKNFALVQLKIIISLVVSKFRFSLSPEYRHSPSYRIIVEPDDGVKIVFQRL 534
           LGKNFALVQLKI+ISL+VSKFRFSLS EYRHSPSYR+IVEP +G+KI+F+R+
Sbjct: 481 LGKNFALVQLKIVISLIVSKFRFSLSSEYRHSPSYRMIVEPANGMKIIFKRV 529

BLAST of Spg028575 vs. NCBI nr
Match: XP_038893045.1 (cytochrome P450 714A1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 828.2 bits (2138), Expect = 4.1e-236
Identity = 413/535 (77.20%), Postives = 460/535 (85.98%), Query Frame = 0

Query: 1   MEGALLDGLVSIFPAGVAMAVVAVLVHFY-YGVWRRTAEVRRKLGAQGVTGPPPSSALFG 60
           MEG+++ G +S+FP  V + VVA+LVH Y YG WRRTAEVRRKL AQGVTGPPP S L+G
Sbjct: 6   MEGSIVCGFISVFPVAVTVTVVAILVHLYFYGAWRRTAEVRRKLRAQGVTGPPP-SILYG 65

Query: 61  NLPEMKEIELQAAAMASPPHHPSPTVAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLY 120
           NLPEM++I+LQ AAMASPPH  S  VAHDY STLFPYFV+WRKQYGPLYTYSTGTRQHLY
Sbjct: 66  NLPEMQKIQLQTAAMASPPHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLY 125

Query: 121 ANDVDLVKDLSLSKNPDLDKPSYIFKKFAPLLGYGVILSNGADWTLQHKIIAPEFFMDRV 180
           AN V+LVKDLSLS N +L KP Y+ KK AP+LG+ ++ SNGA W +Q KIIAPEFFMDRV
Sbjct: 126 ANKVELVKDLSLSNNLNLGKPFYVTKKLAPILGHSIVRSNGAIWAVQRKIIAPEFFMDRV 185

Query: 181 RGMAALMVEAAAPMLEKWEGRIGEEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGK 240
           R MA LM +AA+ ++EKWE RI   G+GA+ EIEV+E LRGFSADVISRACFGSSYEKGK
Sbjct: 186 RAMAVLMADAASSLVEKWESRI---GDGASVEIEVDEDLRGFSADVISRACFGSSYEKGK 245

Query: 241 QIFSKLRDLQKLISDGNPLF-RSSFSDRFFQPSKHRRIKKLEKEIESLIWETVQQRQEEC 300
           +IFSKLRDLQKLIS+G+ LF  SS SDRF QP KH+RIKKLEKEIESLIWETVQQRQ+E 
Sbjct: 246 EIFSKLRDLQKLISEGSFLFGYSSLSDRFLQPRKHKRIKKLEKEIESLIWETVQQRQKEF 305

Query: 301 SKTSSSEKDLLQVLMEGAMNDPTIGACNESCKKFIVDNCKTMYFAGHESSAATATWALML 360
           SKTSSSEKDLLQ++ME   NDP +GA N+S K FIVDNCK++YFAGHES+A  ATW LML
Sbjct: 306 SKTSSSEKDLLQLIMEATTNDPNVGA-NDSSKNFIVDNCKSIYFAGHESTAVAATWTLML 365

Query: 361 LALHPEWQDRIRSEFTQACPDGRLDTTAISQLKSLSMVVQETLRLYPPGAYVARETFAET 420
           LALHPEWQD IRSEF  ACPDGR DTT IS LK++SMVV E LRLYPP A+VARETFA+T
Sbjct: 366 LALHPEWQDCIRSEFAHACPDGRPDTTVISNLKTVSMVVHEALRLYPPAAFVARETFADT 425

Query: 421 RLGNVVVPKGVCLWTLIPMLHTEVEIWGEDANEFKPERFANGIAKACKFPQAYLPFGAGH 480
           RLGNVVVPKGVCLWTLIP LH EVEIWGEDANEFKPERF NGIAKACKFPQAY+PFGAG 
Sbjct: 426 RLGNVVVPKGVCLWTLIPTLHREVEIWGEDANEFKPERFKNGIAKACKFPQAYVPFGAGP 485

Query: 481 RTCLGKNFALVQLKIIISLVVSKFRFSLSPEYRHSPSYRIIVEPDDGVKIVFQRL 534
           R CLGKNFALVQLKIIISL+VSKFRFSLSPEY H PSYR+IVEP +GVKI FQRL
Sbjct: 486 RLCLGKNFALVQLKIIISLIVSKFRFSLSPEYCHCPSYRMIVEPANGVKIAFQRL 535

BLAST of Spg028575 vs. ExPASy Swiss-Prot
Match: Q93Z79 (Cytochrome P450 714A1 OS=Arabidopsis thaliana OX=3702 GN=CYP714A1 PE=2 SV=1)

HSP 1 Score: 552.0 bits (1421), Expect = 7.5e-156
Identity = 281/519 (54.14%), Postives = 371/519 (71.48%), Query Frame = 0

Query: 17  VAMAVVAVLVHFYYGVWRRTAEVRRKLGAQGVTGPPPSSALF-GNLPEMKEIELQAAAMA 76
           V + V+ + +  Y  V      +RRKL  QGV GPPPS  LF GN+PEM++I  Q+  M+
Sbjct: 16  VVIGVLGLGIRVYGKVMAEQWRMRRKLTMQGVKGPPPS--LFRGNVPEMQKI--QSQIMS 75

Query: 77  SPPHHPSPT-VAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLYANDVDLVKDLSLSKN 136
           +  H+     +AHDY S+LFPY   WRKQYG +YTYSTG +QHLY N  +LVK+L+ +  
Sbjct: 76  NSKHYSGDNIIAHDYTSSLFPYLDHWRKQYGRVYTYSTGVKQHLYMNHPELVKELNQANT 135

Query: 137 PDLDKPSYIFKKFAPLLGYGVILSNGADWTLQHKIIAPEFFMDRVRGMAALMVEAAAPML 196
            +L K SY+ K+   +LG GVI SNG  W  Q +IIAPEFF+D+V+GM  L+VE+A PML
Sbjct: 136 LNLGKVSYVTKRLKSILGRGVITSNGPHWAHQRRIIAPEFFLDKVKGMVGLVVESAMPML 195

Query: 197 EKWEGRIGEEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGKQIFSKLRDLQKLISD 256
            KWE  +  EGE    +I V+E LR  SADVISRACFGSS+ KGK+IFSKLR LQK I+ 
Sbjct: 196 SKWEEMMKREGE-MVCDIIVDEDLRAASADVISRACFGSSFSKGKEIFSKLRCLQKAITH 255

Query: 257 GNPLFR-SSFSDRFFQPSKH--RRIKKLEKEIESLIWETVQQRQEECSKTSSSEKDLLQV 316
            N LF  + F+D  F   KH   +I +LE+ IESLIWETV++R+ EC      +KDL+Q+
Sbjct: 256 NNILFSLNGFTDVVFGTKKHGNGKIDELERHIESLIWETVKEREREC--VGDHKKDLMQL 315

Query: 317 LMEGAMN--DPTIGACNESCKKFIVDNCKTMYFAGHESSAATATWALMLLALHPEWQDRI 376
           ++EGA +  D  +    +S K F+VDNCK++YFAGHE+SA   +W LMLLAL+P WQ RI
Sbjct: 316 ILEGARSSCDGNLEDKTQSYKSFVVDNCKSIYFAGHETSAVAVSWCLMLLALNPSWQTRI 375

Query: 377 RSEFTQACPDGRLDTTAISQLKSLSMVVQETLRLYPPGAYVARETFAETRLGNVVVPKGV 436
           R E    C +G  D  +IS LK+++MV+QETLRLYPP A+V+RE   +T+LGN+VVPKGV
Sbjct: 376 RDEVFLHCKNGIPDADSISNLKTVTMVIQETLRLYPPAAFVSREALEDTKLGNLVVPKGV 435

Query: 437 CLWTLIPMLHTEVEIWGEDANEFKPERFANGIAKACKFPQAYLPFGAGHRTCLGKNFALV 496
           C+WTLIP LH + EIWG DANEF PERF+ G++KACK PQ+++PFG G R CLGKNF ++
Sbjct: 436 CIWTLIPTLHRDPEIWGADANEFNPERFSEGVSKACKHPQSFVPFGLGTRLCLGKNFGMM 495

Query: 497 QLKIIISLVVSKFRFSLSPEYRHSPSYRIIVEPDDGVKI 529
           +LK+++SL+VS+F F+LSP Y+HSP +R++VEP  GV I
Sbjct: 496 ELKVLVSLIVSRFSFTLSPTYQHSPVFRMLVEPQHGVVI 527

BLAST of Spg028575 vs. ExPASy Swiss-Prot
Match: Q6NKZ8 (Cytochrome P450 714A2 OS=Arabidopsis thaliana OX=3702 GN=CYP714A2 PE=2 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 1.7e-144
Identity = 258/516 (50.00%), Postives = 357/516 (69.19%), Query Frame = 0

Query: 17  VAMAVVAVLVHFYYGVWRRTAEVRRKLGAQGVTGPPPSSALFGNLPEMKEIELQAAAMAS 76
           V + + +V  H Y         +RR L  QGV GPPP S   GN+ EM+ I+ +A   + 
Sbjct: 16  VIVGIFSVGYHVYGRAVVEQWRMRRSLKLQGVKGPPP-SIFNGNVSEMQRIQSEAKHCSG 75

Query: 77  PPHHPSPTVAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLYANDVDLVKDLSLSKNPD 136
                   ++HDY S+LFP+F  WRKQYG +YTYSTG +QHLY N  ++VK+LS +   +
Sbjct: 76  -----DNIISHDYSSSLFPHFDHWRKQYGRIYTYSTGLKQHLYINHPEMVKELSQTNTLN 135

Query: 137 LDKPSYIFKKFAPLLGYGVILSNGADWTLQHKIIAPEFFMDRVRGMAALMVEAAAPMLEK 196
           L + ++I K+  P+LG G+I SNG  W  Q +IIA EF  D+++GM  LMVE+A PML K
Sbjct: 136 LGRITHITKRLNPILGNGIITSNGPHWAHQRRIIAYEFTHDKIKGMVGLMVESAMPMLNK 195

Query: 197 WEGRIGEEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGKQIFSKLRDLQKLISDGN 256
           WE  +   GE    +I V+E L+  SADVI++ACFGSS+ KGK IFS +RDL   I+  +
Sbjct: 196 WEEMVKRGGE-MGCDIRVDEDLKDVSADVIAKACFGSSFSKGKAIFSMIRDLLTAITKRS 255

Query: 257 PLFR-SSFSDRFFQPSKHR--RIKKLEKEIESLIWETVQQRQEECSKTSSSEKDLLQVLM 316
            LFR + F+D  F   KH    I  LE E+ES IWETV++R+ EC  T   +KDL+Q+++
Sbjct: 256 VLFRFNGFTDMVFGSKKHGDVDIDALEMELESSIWETVKEREIECKDT--HKKDLMQLIL 315

Query: 317 EGAMNDPTIGACNESC-KKFIVDNCKTMYFAGHESSAATATWALMLLALHPEWQDRIRSE 376
           EGAM        ++S  ++F+VDNCK++YFAGH+S+A + +W LMLLAL+P WQ +IR E
Sbjct: 316 EGAMRSCDGNLWDKSAYRRFVVDNCKSIYFAGHDSTAVSVSWCLMLLALNPSWQVKIRDE 375

Query: 377 FTQACPDGRLDTTAISQLKSLSMVVQETLRLYPPGAYVARETFAETRLGNVVVPKGVCLW 436
              +C +G  D  +I  LK+++MV+QET+RLYPP   V RE   + RLG++VVPKGVC+W
Sbjct: 376 ILSSCKNGIPDAESIPNLKTVTMVIQETMRLYPPAPIVGREASKDIRLGDLVVPKGVCIW 435

Query: 437 TLIPMLHTEVEIWGEDANEFKPERFANGIAKACKFPQAYLPFGAGHRTCLGKNFALVQLK 496
           TLIP LH + EIWG DAN+FKPERF+ GI+KACK+PQ+Y+PFG G RTC+GKNF ++++K
Sbjct: 436 TLIPALHRDPEIWGPDANDFKPERFSEGISKACKYPQSYIPFGLGPRTCVGKNFGMMEVK 495

Query: 497 IIISLVVSKFRFSLSPEYRHSPSYRIIVEPDDGVKI 529
           +++SL+VSKF F+LSP Y+HSPS++++VEP  GV I
Sbjct: 496 VLVSLIVSKFSFTLSPTYQHSPSHKLLVEPQHGVVI 522

BLAST of Spg028575 vs. ExPASy Swiss-Prot
Match: Q2QYH7 (Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 3.0e-128
Identity = 237/512 (46.29%), Postives = 330/512 (64.45%), Query Frame = 0

Query: 22  VAVLVHFYYGVWRRTAEVRRKLGAQGVTGPPPSSALFGNLPEMKEIELQAAAMASPPHHP 81
           + +  + Y  +W R   +R+KL +QGV GP P S LFGN+PEM+ I+  A +        
Sbjct: 20  ILLFSYVYIILWLRPERLRQKLRSQGVRGPKP-SFLFGNIPEMRRIQQLAKSAHEQEAGS 79

Query: 82  SPTVAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLYANDVDLVKDLSLSKNPDLDKPS 141
           +   + +Y++TLFPYF+ W + YG +Y YSTG+ Q L   D ++VK+L+  K+ DL KP 
Sbjct: 80  TDMFSSNYVATLFPYFLHWSRVYGSIYLYSTGSIQVLNVTDPNMVKELANCKSLDLGKPC 139

Query: 142 YIFKKFAPLLGYGVILSNGADWTLQHKIIAPEFFMDRVRGMAALMVEAAAPMLEKWEGRI 201
           Y+ K+   LLG G++ SNG  W  Q K+IAPE FM+RV+GM  LM+EAA  ML  W+  +
Sbjct: 140 YLQKERGALLGMGILTSNGDLWVHQRKVIAPELFMERVKGMVNLMMEAAMSMLNSWKNEV 199

Query: 202 GEEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGKQIFSKLRDLQKLISDGNPLFRS 261
             E  G +AEI V+E LR FSADVISRACFGSS+ +GK+IF K+R LQK ++  + L   
Sbjct: 200 --EDRGGSAEIVVDEFLRTFSADVISRACFGSSFSEGKEIFIKIRQLQKAMAKQSMLIGV 259

Query: 262 SFSDRFFQPSKHRRIKKLEKEIESLIWETVQQRQEECSKTSSSEKDLLQVLMEGAMNDPT 321
             S R+     +R I  L+  I +LI   + ++ E  S TS + KDLL  +++G+ + P 
Sbjct: 260 PGS-RYLPTRSNRGIWNLDSSIRTLIL-NISKKYEHDSSTSVN-KDLLHSIIQGSKDGPF 319

Query: 322 IGACNESCKKFIVDNCKTMYFAGHESSAATATWALMLLALHPEWQDRIRSEFTQACPDGR 381
                E    FIVDNCK +YFAGHE+++ TA W LMLLA H EWQ R R E    C    
Sbjct: 320 ASCTPED---FIVDNCKNIYFAGHETTSTTAAWCLMLLASHHEWQSRARVESLDICQGRP 379

Query: 382 LDTTAISQLKSLSMVVQETLRLYPPGAYVARETFAETRLGNVVVPKGVCLWTLIPMLHTE 441
           LD   + +LK L+MV+QETLRLYPP ++VARE   + +LG + +PKG  +W  I M H +
Sbjct: 380 LDFDILRKLKKLTMVIQETLRLYPPASFVAREALNDMKLGGIDIPKGTNIWIPIAMAHRD 439

Query: 442 VEIWGEDANEFKPERFANGIAKACKFPQAYLPFGAGHRTCLGKNFALVQLKIIISLVVSK 501
             +WG  A++F P+RFANGIA ACK P  Y+PFG G RTC G+N A+V+LK+++SL++SK
Sbjct: 440 PSVWGPSADKFDPDRFANGIAGACKPPHMYMPFGVGVRTCAGQNLAMVELKVVLSLLLSK 499

Query: 502 FRFSLSPEYRHSPSYRIIVEPDDGVKIVFQRL 534
           F F LSP Y H P++R+ +EP  GV ++F+ L
Sbjct: 500 FEFKLSPNYVHCPAFRLTIEPGKGVPLIFREL 522

BLAST of Spg028575 vs. ExPASy Swiss-Prot
Match: Q5KQH7 (Cytochrome P450 714D1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714D1 PE=1 SV=1)

HSP 1 Score: 449.9 bits (1156), Expect = 4.0e-125
Identity = 252/568 (44.37%), Postives = 344/568 (60.56%), Query Frame = 0

Query: 1   MEGALLDGLVSIFPAGVAMAVVAVLVHFYYGVW----RRTAEVRRKLGAQGVTGPPPSSA 60
           ME   +    +  P  VA AV+A L       W    RR AEV R+   QG+ GPPPSS 
Sbjct: 1   MESFFVFFTAAALPVVVAAAVIAGLC--ITAAWLARPRRVAEVFRR---QGIDGPPPSSF 60

Query: 61  LFGNLPEMKEIELQAAAMASPPHHPSPTVA---------------HDYISTLFPYFVKWR 120
           L GNLPEMK     AA+ A+P      T +                DY + +FPYF KWR
Sbjct: 61  LAGNLPEMKARVAAAASAAAPTADGEETASAGGGGGGRDFEKDGFDDYCTRIFPYFHKWR 120

Query: 121 KQYGPLYTYSTGTRQHLYANDVDLVKDLSLSKNPDLDKPSYIFKKFAPLLGYGVILSNGA 180
           K YG  Y Y    R  LY  D +L+ ++    + D+ KP Y+ K   PL G GV+ +NGA
Sbjct: 121 KAYGETYLYWLRRRPALYVTDPELIGEIGRCVSLDMGKPKYLQKGQEPLFGGGVLKANGA 180

Query: 181 DWTLQHKIIAPEFFMDRVRGMAALMVEAAAPMLEKWEGRIGEEGEGAAAEIEVEEGLRGF 240
            W  Q K+IAPEF+M RVR M  LMV+AA P++  WE RI   G  AAAE+ V+  LR F
Sbjct: 181 CWARQRKVIAPEFYMARVRAMVQLMVDAAQPLIASWESRIDAAGGAAAAEVVVDGDLRSF 240

Query: 241 SADVISRACFGSSYEKGKQIFSKLRDLQKLISDGNPLFRSSFSDRFFQPSKHRRIKKLEK 300
           S DVISRACFGS Y +G++IF +LR+L  L+S+ + +F S  S R     K+RRI +L  
Sbjct: 241 SFDVISRACFGSDYSRGREIFLRLRELSGLMSETSVIF-SIPSLRHLPTGKNRRIWRLTG 300

Query: 301 EIESLIWETVQQRQ----------EECSKTSSSEKDLLQVLMEGAMNDPTIGACNESCKK 360
           EI SLI E V++R+           + +  S  E+D L  ++E +   P           
Sbjct: 301 EIRSLIMELVRERRCAARAAREHGGKAAPPSPPERDFLGSIIENSGGQP-------RPDD 360

Query: 361 FIVDNCKTMYFAGHESSAATATWALMLLALHPEWQDRIRSEFTQAC-PDGRL-----DTT 420
           F+VDNCK +YFAGHE+SA TATW LMLLA HPEWQDR R+E  + C  DG       D  
Sbjct: 361 FVVDNCKNIYFAGHETSAVTATWCLMLLAAHPEWQDRARAEVLEVCGGDGAAAPAAPDFD 420

Query: 421 AISQLKSLSMVVQETLRLYPPGAYVARETFAETRLGNVVVPKGVCLWTLIPMLHTEVEIW 480
            +S+++++ MVVQETLRL+PP ++V RETF + +LG ++ PKG  L+  +  +H +V  W
Sbjct: 421 MVSRMRTVGMVVQETLRLFPPSSFVVRETFRDMQLGRLLAPKGTYLFVPVSTMHHDVAAW 480

Query: 481 GEDANEFKPERFANGIAKACKFPQA-YLPFGAGHRTCLGKNFALVQLKIIISLVVSKFRF 533
           G  A  F P RF +G+A ACK PQA ++PFG G RTCLG+N ALV++K ++++V+++F F
Sbjct: 481 GPTARLFDPSRFRDGVAAACKHPQASFMPFGLGARTCLGQNLALVEVKTLVAVVLARFEF 540

BLAST of Spg028575 vs. ExPASy Swiss-Prot
Match: B6SSW8 (Cytochrome P450 714B3 OS=Zea mays OX=4577 GN=CYP714B3 PE=2 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 3.9e-120
Identity = 225/511 (44.03%), Postives = 328/511 (64.19%), Query Frame = 0

Query: 26  VHFYYGVWRRTAEVRRKLGAQGVTGPPPSSALFGNLPEMKEIELQA-AAMASPPHHPS-- 85
           V+ Y+ +W    +V  K   Q + GP PS   +GNL +M+E    A AA AS     S  
Sbjct: 25  VYLYHILWLVPQKVLAKFEDQKIGGPRPSFP-YGNLADMREAAAAAKAARASARRSGSGG 84

Query: 86  PTVAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLYANDVDLVKDLSLSKNPDLDKPSY 145
             + HDY   + PY+ KWRK+YGP++TYS G    L+ +  D+V+D++L  + DL K SY
Sbjct: 85  GGIVHDYRPAVLPYYEKWRKEYGPIFTYSMGNVVFLHVSRPDVVRDINLCVSLDLGKSSY 144

Query: 146 IFKKFAPLLGYGVILSNGADWTLQHKIIAPEFFMDRVRGMAALMVEAAAPMLEKWEGRIG 205
           +     PL G G++ SNG  W  Q KIIAPEFF+D+V+GM  LMV++A P+L  WE R+ 
Sbjct: 145 LKATHEPLFGGGILKSNGEAWLHQRKIIAPEFFLDKVKGMVDLMVDSAQPLLMSWEERV- 204

Query: 206 EEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGKQIFSKLRDLQKLISDGNPLFRSS 265
            +  G   +I++++ +R +SADVISR CFGSSY KGK+IF K+R+LQ+ +S  N L   +
Sbjct: 205 -DRNGGITDIKIDDDIRAYSADVISRTCFGSSYIKGKEIFMKIRELQQAVSKPNVLAEMT 264

Query: 266 FSDRFFQPSKHRRIKKLEKEIESLIWETVQQRQEECSKTSSSEKDLLQVLMEGAMNDPTI 325
              RFF   ++++  +L K++  LI E V        K S  +++LL  ++  A +   +
Sbjct: 265 -GLRFFPSMRNKQAWELHKQVRKLILEIV--------KESGEDRNLLSAILHSA-STSRV 324

Query: 326 GACNESCKKFIVDNCKTMYFAGHESSAATATWALMLLALHPEWQDRIRSEFTQACPDGRL 385
           G      + FIVDNCK++YFAGHES+A TA W LMLL LHPEWQ+R+R E  + C    +
Sbjct: 325 GIA--EAENFIVDNCKSIYFAGHESTAVTAAWCLMLLGLHPEWQNRVREEVHEVCRGQPV 384

Query: 386 DTTAISQLKSLSMVVQETLRLYPPGAYVARETFAETRLGNVVVPKGVCLWTLIPMLHTEV 445
           D+ ++ ++K+L+MV+QETLRLYP GA+V+R+   E +LG V +PKGV ++  +  +H + 
Sbjct: 385 DSRSLQKMKNLTMVIQETLRLYPAGAFVSRQALQELKLGGVHIPKGVNIYIPVSTMHLDP 444

Query: 446 EIWGEDANEFKPERFANGIAKACKFPQAYLPFGAGHRTCLGKNFALVQLKIIISLVVSKF 505
           E+WG D  EF PERF++   +      +YLPFGAG RTCLG+ FA+ +LKI+ISL+VSKF
Sbjct: 445 ELWGPDVKEFNPERFSDVRPQL----HSYLPFGAGARTCLGQGFAMAELKILISLIVSKF 504

Query: 506 RFSLSPEYRHSPSYRIIVEPDDGVKIVFQRL 534
              LSP Y+HSP+ ++IVEP+ GV +   ++
Sbjct: 505 VLKLSPHYQHSPTLKLIVEPELGVDLTLTKV 516

BLAST of Spg028575 vs. ExPASy TrEMBL
Match: A0A6J1K5V5 (cytochrome P450 714A1-like OS=Cucurbita maxima OX=3661 GN=LOC111490994 PE=3 SV=1)

HSP 1 Score: 829.3 bits (2141), Expect = 9.0e-237
Identity = 419/531 (78.91%), Postives = 467/531 (87.95%), Query Frame = 0

Query: 6   LDGLVSIFPAGVAMAVVAVLVHFYYGVWRRTAEVRRKLGAQGVTGPPPSSALFGNLPEMK 65
           + GL S+  A VA+AVVAV+VHFYYGVWRRTAE+RRKL AQGVTGPPP S L+GNL EM+
Sbjct: 1   MGGLGSVLFAAVAVAVVAVVVHFYYGVWRRTAEIRRKLRAQGVTGPPP-SLLYGNLQEMQ 60

Query: 66  EIELQ-AAAMASPPHHPSPTVAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLYANDVD 125
           EI+L+ AAAM SPPHH S  VAHDY STLFPYFVKWRKQYGPLYTYSTGTRQHLYAN VD
Sbjct: 61  EIQLEAAAAMVSPPHHASVIVAHDYTSTLFPYFVKWRKQYGPLYTYSTGTRQHLYANHVD 120

Query: 126 LVKDLSLSKNPDLDKPSYIFKKFAPLLGYGVILSNGADWTLQHKIIAPEFFMDRVRGMAA 185
           LVKDLSL+    L KPSY+ KK AP+LG+ ++ +NG  W LQ KIIAPEFFMD+VR MA 
Sbjct: 121 LVKDLSLTNYLHLGKPSYVTKKLAPILGHSIVRANGPVWALQRKIIAPEFFMDQVRVMAT 180

Query: 186 LMVEAAAPMLEKWEGRIGEEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGKQIFSK 245
           LM EAAA ML+KWEGRIG EGEGA AEI+V+E LR FSADVISRACFGSSYEKGK+IFSK
Sbjct: 181 LMTEAAAAMLQKWEGRIG-EGEGATAEIDVDEDLREFSADVISRACFGSSYEKGKRIFSK 240

Query: 246 LRDLQKLISDGNPLF-RSSFSDRFFQPSKHRRIKKLEKEIESLIWETVQQRQEECSKTSS 305
           LR LQKL+S+GN LF   S SDR FQ SKHRRIKKLEKEIESLIWETVQQRQ+ECSKT S
Sbjct: 241 LRVLQKLLSEGNFLFGYVSLSDRLFQQSKHRRIKKLEKEIESLIWETVQQRQKECSKT-S 300

Query: 306 SEKDLLQVLMEGAMNDPTIGA-CNESCKKFIVDNCKTMYFAGHESSAATATWALMLLALH 365
           S+KDLLQ++ME AMNDPT+ A CNES KKFIVDNCK++YFAGHES+A  ATW+LMLLALH
Sbjct: 301 SDKDLLQLIMEAAMNDPTVAANCNESPKKFIVDNCKSIYFAGHESTAVAATWSLMLLALH 360

Query: 366 PEWQDRIRSEFTQACPDGRLDTTAISQLKSLSMVVQETLRLYPPGAYVARETFAETRLGN 425
           PEWQDRIRSEF QAC DGR++ T ISQLKS+SMVVQETLRLYPP A+VARETF+ETRLGN
Sbjct: 361 PEWQDRIRSEFGQACLDGRVNMTTISQLKSVSMVVQETLRLYPPAAFVARETFSETRLGN 420

Query: 426 VVVPKGVCLWTLIPMLHTEVEIWGEDANEFKPERFANGIAKACKFPQAYLPFGAGHRTCL 485
           VV+PKG+ LWTLIP LH EVEIWGE+ANEFKPERF NGIAKACKFPQAYLPFGAG R CL
Sbjct: 421 VVIPKGIYLWTLIPTLHREVEIWGENANEFKPERFVNGIAKACKFPQAYLPFGAGPRLCL 480

Query: 486 GKNFALVQLKIIISLVVSKFRFSLSPEYRHSPSYRIIVEPDDGVKIVFQRL 534
           GKNFALVQLKI+ISL+VSKFRFSLSPEYRHSPSYR+IVEP +G+KI+F+R+
Sbjct: 481 GKNFALVQLKIVISLIVSKFRFSLSPEYRHSPSYRMIVEPANGMKIIFKRV 528

BLAST of Spg028575 vs. ExPASy TrEMBL
Match: A0A6J1FRZ8 (cytochrome P450 714A1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111447915 PE=3 SV=1)

HSP 1 Score: 828.6 bits (2139), Expect = 1.5e-236
Identity = 418/532 (78.57%), Postives = 469/532 (88.16%), Query Frame = 0

Query: 6   LDGLVSIFPAGVAMAVVAVLVHFYYGVWRRTAEVRRKLGAQGVTGPPPSSALFGNLPEMK 65
           + GL S+    VA+AVVAV+VHFYYGVWRRTAE+RRKL AQGVTGP P S L+GNLPEM+
Sbjct: 1   MGGLGSVLFTAVAVAVVAVVVHFYYGVWRRTAEIRRKLRAQGVTGPSP-SLLYGNLPEMQ 60

Query: 66  EIELQ--AAAMASPPHHPSPTVAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLYANDV 125
           EI+L+  AAAMASPPHH S  VAHDY STLFPYFVKWRKQYGPLYTYSTGTRQHLYAN V
Sbjct: 61  EIQLEAAAAAMASPPHHASVIVAHDYTSTLFPYFVKWRKQYGPLYTYSTGTRQHLYANHV 120

Query: 126 DLVKDLSLSKNPDLDKPSYIFKKFAPLLGYGVILSNGADWTLQHKIIAPEFFMDRVRGMA 185
           DLVKDLSL+ +  L KPSY+ KK AP+LG+ ++ +NG  W LQ KIIAPEFFMDRVR MA
Sbjct: 121 DLVKDLSLTNHLHLGKPSYVTKKLAPILGHSIVRANGPVWALQRKIIAPEFFMDRVRVMA 180

Query: 186 ALMVEAAAPMLEKWEGRIGEEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGKQIFS 245
           ALM EAAA ML+KWE RI +EG+GA AEI+V+E LR FSADVISRACFGSSYEKGK+IFS
Sbjct: 181 ALMTEAAAAMLQKWEERI-DEGDGATAEIDVDEALREFSADVISRACFGSSYEKGKRIFS 240

Query: 246 KLRDLQKLISDGNPLF-RSSFSDRFFQPSKHRRIKKLEKEIESLIWETVQQRQEECSKTS 305
           KLR LQKL+S+GN LF   S SDR FQ SKHR IKKLEKEIESLIWETVQQRQ+ECSKT 
Sbjct: 241 KLRVLQKLLSEGNFLFGYVSLSDRLFQQSKHRTIKKLEKEIESLIWETVQQRQKECSKT- 300

Query: 306 SSEKDLLQVLMEGAMNDPTIGA-CNESCKKFIVDNCKTMYFAGHESSAATATWALMLLAL 365
           SS+KDLLQ++ME AMNDPT+G+ CNES KKFIVDNCK++YFAGHES+A  ATW+LMLLAL
Sbjct: 301 SSDKDLLQLIMEAAMNDPTVGSNCNESPKKFIVDNCKSIYFAGHESTAVAATWSLMLLAL 360

Query: 366 HPEWQDRIRSEFTQACPDGRLDTTAISQLKSLSMVVQETLRLYPPGAYVARETFAETRLG 425
           HPEWQDRIRSEFTQACPDGR++ TAISQLKS SMVVQETLRLYPP A+VARETF+ETRLG
Sbjct: 361 HPEWQDRIRSEFTQACPDGRVNMTAISQLKSASMVVQETLRLYPPAAFVARETFSETRLG 420

Query: 426 NVVVPKGVCLWTLIPMLHTEVEIWGEDANEFKPERFANGIAKACKFPQAYLPFGAGHRTC 485
           NVV+PKGV LWTLIP LH EVEIWGE+AN+FKPERF NGIAKACKFPQAY+PFGAG R C
Sbjct: 421 NVVIPKGVYLWTLIPTLHREVEIWGENANKFKPERFVNGIAKACKFPQAYVPFGAGPRLC 480

Query: 486 LGKNFALVQLKIIISLVVSKFRFSLSPEYRHSPSYRIIVEPDDGVKIVFQRL 534
           LGKNFALVQLKI+ISL+VSKFRFSLS EYRHSPSYR+IVEP +G+KI+F+R+
Sbjct: 481 LGKNFALVQLKIVISLIVSKFRFSLSSEYRHSPSYRMIVEPANGMKIIFKRV 529

BLAST of Spg028575 vs. ExPASy TrEMBL
Match: A0A0A0KQQ7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G224130 PE=3 SV=1)

HSP 1 Score: 809.7 bits (2090), Expect = 7.4e-231
Identity = 401/530 (75.66%), Postives = 455/530 (85.85%), Query Frame = 0

Query: 6   LDGLVSIFPAGVAMAVVAVLVHFY-YGVWRRTAEVRRKLGAQGVTGPPPSSALFGNLPEM 65
           + G V +FP  + +AVVA+L+HFY YG WRRTAEVRRKL AQGVTGPPP S L+GNLPEM
Sbjct: 1   MSGFVLVFPTAIVVAVVAILLHFYSYGAWRRTAEVRRKLEAQGVTGPPP-SILYGNLPEM 60

Query: 66  KEIELQAAAMASPPHHPSPTVAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLYANDVD 125
           ++I+LQ AAMASP H  S  VAHDY STLFPYFV+WRKQYGPLYTYSTGTRQHLYAN V+
Sbjct: 61  QKIQLQTAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYANKVE 120

Query: 126 LVKDLSLSKNPDLDKPSYIFKKFAPLLGYGVILSNGADWTLQHKIIAPEFFMDRVRGMAA 185
           LVKDLSLS N ++ KP Y+ KK AP+LG  V+ SNGA W +Q K+IAPEFFMDRVR MA 
Sbjct: 121 LVKDLSLSNNLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVRAMAF 180

Query: 186 LMVEAAAPMLEKWEGRIGEEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGKQIFSK 245
            MV+AA+ ++ KWE R+G+       EI+V+E LRGFSADVISRACFGSSYEKGK+IFSK
Sbjct: 181 HMVDAASCLINKWESRVGD----GTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSK 240

Query: 246 LRDLQKLISDGNPLF-RSSFSDRFFQPSKHRRIKKLEKEIESLIWETVQQRQEECSKTSS 305
           LRDLQKLI + + LF  SS+SDRF QP KH+RI +LEKEIESLIWETVQQRQ+ECSKTSS
Sbjct: 241 LRDLQKLICEDSFLFGYSSWSDRFLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTSS 300

Query: 306 SEKDLLQVLMEGAMNDPTIGACNESCKKFIVDNCKTMYFAGHESSAATATWALMLLALHP 365
           S+KDLLQ++ME  ++DP IGA  +S K FIVDNCK++YFAGHES+A  ATW+LMLLALHP
Sbjct: 301 SDKDLLQLIMEATIDDPNIGA-KDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHP 360

Query: 366 EWQDRIRSEFTQACPDGRLDTTAISQLKSLSMVVQETLRLYPPGAYVARETFAETRLGNV 425
           EWQDRIRSEF QACPDG LD TA SQLKS+SMV+ ETLRLYPP A+VARETFAET+LGNV
Sbjct: 361 EWQDRIRSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNV 420

Query: 426 VVPKGVCLWTLIPMLHTEVEIWGEDANEFKPERFANGIAKACKFPQAYLPFGAGHRTCLG 485
           VVPKGVCLWTLIP LH EVEIWGEDANEFKPERFANG+AKACKFPQAY+PFGAG R CLG
Sbjct: 421 VVPKGVCLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLG 480

Query: 486 KNFALVQLKIIISLVVSKFRFSLSPEYRHSPSYRIIVEPDDGVKIVFQRL 534
           KNFALV+LKIIISL+VSKFRFSLSPEY H PSYR+IVEP +GVKIVFQRL
Sbjct: 481 KNFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL 524

BLAST of Spg028575 vs. ExPASy TrEMBL
Match: A0A1S3CNV5 (cytochrome P450 714A1-like OS=Cucumis melo OX=3656 GN=LOC103502912 PE=3 SV=1)

HSP 1 Score: 808.9 bits (2088), Expect = 1.3e-230
Identity = 399/535 (74.58%), Postives = 459/535 (85.79%), Query Frame = 0

Query: 1   MEGALLDGLVSIFPAGVAMAVVAVLVHFY-YGVWRRTAEVRRKLGAQGVTGPPPSSALFG 60
           MEG ++ G V +FP  V MAVV +L+HFY YG WRRTA+VRRKL AQGVTGPPP S L+G
Sbjct: 1   MEGYVISGFVLVFPTAVVMAVVMILLHFYFYGAWRRTADVRRKLRAQGVTGPPP-SILYG 60

Query: 61  NLPEMKEIELQAAAMASPPHHPSPTVAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLY 120
           NLPEM++I+LQ AAMASP H  S  VAHDY STLFPYFV+WRKQYGPLYTYSTGTRQHLY
Sbjct: 61  NLPEMQKIQLQTAAMASPLHCASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLY 120

Query: 121 ANDVDLVKDLSLSKNPDLDKPSYIFKKFAPLLGYGVILSNGADWTLQHKIIAPEFFMDRV 180
           AN V+LVKDLSLS N ++ KP Y+ +K AP+LG  V+ SNG+ W +Q K+IAPEFFMDRV
Sbjct: 121 ANKVELVKDLSLSNNLNVGKPFYVTRKLAPILGQSVVRSNGSVWAVQRKVIAPEFFMDRV 180

Query: 181 RGMAALMVEAAAPMLEKWEGRIGEEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGK 240
           R M   MV+AA+ ++ KWE R+G+       EI+V+E LRGFSADVISRACFGSSYEKGK
Sbjct: 181 RAMGFHMVDAASCLINKWERRVGD----GTVEIDVDEDLRGFSADVISRACFGSSYEKGK 240

Query: 241 QIFSKLRDLQKLISDGNPLF-RSSFSDRFFQPSKHRRIKKLEKEIESLIWETVQQRQEEC 300
           +IFSKLRDLQKLIS+ + LF  SS+SDRF +P KH+ IK+LEKEIES+IWETVQQRQ+EC
Sbjct: 241 EIFSKLRDLQKLISEDSFLFGYSSWSDRFLRPRKHKSIKRLEKEIESIIWETVQQRQKEC 300

Query: 301 SKTSSSEKDLLQVLMEGAMNDPTIGACNESCKKFIVDNCKTMYFAGHESSAATATWALML 360
           SKTSSS+KDLLQ++ME  +NDP IGA  +S K FIVDNCK++YFAGHES+A  ATW+LML
Sbjct: 301 SKTSSSDKDLLQLIMEATINDPNIGA-KDSSKNFIVDNCKSIYFAGHESTAVAATWSLML 360

Query: 361 LALHPEWQDRIRSEFTQACPDGRLDTTAISQLKSLSMVVQETLRLYPPGAYVARETFAET 420
           LALHPEWQDRIRSEF QACPDG LDTTA  QLKS++MV+ ETLRLYPP A+VARETFAET
Sbjct: 361 LALHPEWQDRIRSEFAQACPDGHLDTTATLQLKSVNMVIHETLRLYPPAAFVARETFAET 420

Query: 421 RLGNVVVPKGVCLWTLIPMLHTEVEIWGEDANEFKPERFANGIAKACKFPQAYLPFGAGH 480
           +LGNVVVPKGVCLWTLIPMLH EVEIWGEDAN+FKPERFANG+AKACKFPQAY+PFGAG 
Sbjct: 421 QLGNVVVPKGVCLWTLIPMLHREVEIWGEDANKFKPERFANGVAKACKFPQAYVPFGAGP 480

Query: 481 RTCLGKNFALVQLKIIISLVVSKFRFSLSPEYRHSPSYRIIVEPDDGVKIVFQRL 534
           R CLGKNFALV+LKIIISL+VSKFRFSLSPEY H PSYR++VEP +GVKIVFQRL
Sbjct: 481 RLCLGKNFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMVVEPANGVKIVFQRL 529

BLAST of Spg028575 vs. ExPASy TrEMBL
Match: A0A5A7VH75 (Cytochrome P450 714A1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold14G00950 PE=3 SV=1)

HSP 1 Score: 802.4 bits (2071), Expect = 1.2e-228
Identity = 397/534 (74.34%), Postives = 456/534 (85.39%), Query Frame = 0

Query: 1   MEGALLDGLVSIFPAGVAMAVVAVLVHFY-YGVWRRTAEVRRKLGAQGVTGPPPSSALFG 60
           MEG ++ G V +FP  V MAVV +L+HFY YG WRRTA+VRRKL AQGVTGPPP S L+G
Sbjct: 1   MEGYVISGFVLVFPTAVVMAVVMILLHFYFYGAWRRTADVRRKLRAQGVTGPPP-SILYG 60

Query: 61  NLPEMKEIELQAAAMASPPHHPSPTVAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLY 120
           NLPEM++I+LQ AAMASP H  S  VAHDY STLFPYFV+WRKQYGPLYTYSTGTRQHLY
Sbjct: 61  NLPEMQKIQLQTAAMASPLHCASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLY 120

Query: 121 ANDVDLVKDLSLSKNPDLDKPSYIFKKFAPLLGYGVILSNGADWTLQHKIIAPEFFMDRV 180
           AN V+LVKDLSLS N ++ KP Y+ +K AP+LG  V+ SNG+ W +Q K+IAPEFFMDRV
Sbjct: 121 ANKVELVKDLSLSNNLNVGKPFYVTRKLAPILGQSVVRSNGSVWAVQRKVIAPEFFMDRV 180

Query: 181 RGMAALMVEAAAPMLEKWEGRIGEEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGK 240
           R M   MV+AA+ ++ KWE R+G+       EI+V+E LRGFSADVISRACFGSSYEKGK
Sbjct: 181 RAMGFHMVDAASCLINKWERRVGD----GTVEIDVDEDLRGFSADVISRACFGSSYEKGK 240

Query: 241 QIFSKLRDLQKLISDGNPLFRSSFSDRFFQPSKHRRIKKLEKEIESLIWETVQQRQEECS 300
           +IFSKLRDLQKLIS+ + LF  S S RF +P KH+ IK+LEKEIES+IWETVQQRQ+ECS
Sbjct: 241 EIFSKLRDLQKLISEDSFLFGYS-SWRFLRPRKHKSIKRLEKEIESIIWETVQQRQKECS 300

Query: 301 KTSSSEKDLLQVLMEGAMNDPTIGACNESCKKFIVDNCKTMYFAGHESSAATATWALMLL 360
           KTSSS+KDLLQ++ME  +NDP IGA  +S K FIVDNCK++YFAGHES+A  ATW+LMLL
Sbjct: 301 KTSSSDKDLLQLIMEATINDPNIGA-KDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLL 360

Query: 361 ALHPEWQDRIRSEFTQACPDGRLDTTAISQLKSLSMVVQETLRLYPPGAYVARETFAETR 420
           ALHPEWQDRIRSEF QACPDG LDTTA  QLKS++MV+ ETLRLYPP A+VARETFAET+
Sbjct: 361 ALHPEWQDRIRSEFAQACPDGHLDTTATLQLKSVNMVIHETLRLYPPAAFVARETFAETQ 420

Query: 421 LGNVVVPKGVCLWTLIPMLHTEVEIWGEDANEFKPERFANGIAKACKFPQAYLPFGAGHR 480
           LGNVVVPKGVCLWTLIPMLH EVEIWGEDAN+FKPERFANG+AKACKFPQAY+PFGAG R
Sbjct: 421 LGNVVVPKGVCLWTLIPMLHREVEIWGEDANKFKPERFANGVAKACKFPQAYVPFGAGPR 480

Query: 481 TCLGKNFALVQLKIIISLVVSKFRFSLSPEYRHSPSYRIIVEPDDGVKIVFQRL 534
            CLGKNFALV+LKIIISL+VSKFRFSLSPEY H PSYR++VEP +GVKIVFQRL
Sbjct: 481 LCLGKNFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMVVEPANGVKIVFQRL 527

BLAST of Spg028575 vs. TAIR 10
Match: AT5G24910.1 (cytochrome P450, family 714, subfamily A, polypeptide 1 )

HSP 1 Score: 552.0 bits (1421), Expect = 5.3e-157
Identity = 281/519 (54.14%), Postives = 371/519 (71.48%), Query Frame = 0

Query: 17  VAMAVVAVLVHFYYGVWRRTAEVRRKLGAQGVTGPPPSSALF-GNLPEMKEIELQAAAMA 76
           V + V+ + +  Y  V      +RRKL  QGV GPPPS  LF GN+PEM++I  Q+  M+
Sbjct: 16  VVIGVLGLGIRVYGKVMAEQWRMRRKLTMQGVKGPPPS--LFRGNVPEMQKI--QSQIMS 75

Query: 77  SPPHHPSPT-VAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLYANDVDLVKDLSLSKN 136
           +  H+     +AHDY S+LFPY   WRKQYG +YTYSTG +QHLY N  +LVK+L+ +  
Sbjct: 76  NSKHYSGDNIIAHDYTSSLFPYLDHWRKQYGRVYTYSTGVKQHLYMNHPELVKELNQANT 135

Query: 137 PDLDKPSYIFKKFAPLLGYGVILSNGADWTLQHKIIAPEFFMDRVRGMAALMVEAAAPML 196
            +L K SY+ K+   +LG GVI SNG  W  Q +IIAPEFF+D+V+GM  L+VE+A PML
Sbjct: 136 LNLGKVSYVTKRLKSILGRGVITSNGPHWAHQRRIIAPEFFLDKVKGMVGLVVESAMPML 195

Query: 197 EKWEGRIGEEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGKQIFSKLRDLQKLISD 256
            KWE  +  EGE    +I V+E LR  SADVISRACFGSS+ KGK+IFSKLR LQK I+ 
Sbjct: 196 SKWEEMMKREGE-MVCDIIVDEDLRAASADVISRACFGSSFSKGKEIFSKLRCLQKAITH 255

Query: 257 GNPLFR-SSFSDRFFQPSKH--RRIKKLEKEIESLIWETVQQRQEECSKTSSSEKDLLQV 316
            N LF  + F+D  F   KH   +I +LE+ IESLIWETV++R+ EC      +KDL+Q+
Sbjct: 256 NNILFSLNGFTDVVFGTKKHGNGKIDELERHIESLIWETVKEREREC--VGDHKKDLMQL 315

Query: 317 LMEGAMN--DPTIGACNESCKKFIVDNCKTMYFAGHESSAATATWALMLLALHPEWQDRI 376
           ++EGA +  D  +    +S K F+VDNCK++YFAGHE+SA   +W LMLLAL+P WQ RI
Sbjct: 316 ILEGARSSCDGNLEDKTQSYKSFVVDNCKSIYFAGHETSAVAVSWCLMLLALNPSWQTRI 375

Query: 377 RSEFTQACPDGRLDTTAISQLKSLSMVVQETLRLYPPGAYVARETFAETRLGNVVVPKGV 436
           R E    C +G  D  +IS LK+++MV+QETLRLYPP A+V+RE   +T+LGN+VVPKGV
Sbjct: 376 RDEVFLHCKNGIPDADSISNLKTVTMVIQETLRLYPPAAFVSREALEDTKLGNLVVPKGV 435

Query: 437 CLWTLIPMLHTEVEIWGEDANEFKPERFANGIAKACKFPQAYLPFGAGHRTCLGKNFALV 496
           C+WTLIP LH + EIWG DANEF PERF+ G++KACK PQ+++PFG G R CLGKNF ++
Sbjct: 436 CIWTLIPTLHRDPEIWGADANEFNPERFSEGVSKACKHPQSFVPFGLGTRLCLGKNFGMM 495

Query: 497 QLKIIISLVVSKFRFSLSPEYRHSPSYRIIVEPDDGVKI 529
           +LK+++SL+VS+F F+LSP Y+HSP +R++VEP  GV I
Sbjct: 496 ELKVLVSLIVSRFSFTLSPTYQHSPVFRMLVEPQHGVVI 527

BLAST of Spg028575 vs. TAIR 10
Match: AT5G24900.1 (cytochrome P450, family 714, subfamily A, polypeptide 2 )

HSP 1 Score: 514.2 bits (1323), Expect = 1.2e-145
Identity = 258/516 (50.00%), Postives = 357/516 (69.19%), Query Frame = 0

Query: 17  VAMAVVAVLVHFYYGVWRRTAEVRRKLGAQGVTGPPPSSALFGNLPEMKEIELQAAAMAS 76
           V + + +V  H Y         +RR L  QGV GPPP S   GN+ EM+ I+ +A   + 
Sbjct: 16  VIVGIFSVGYHVYGRAVVEQWRMRRSLKLQGVKGPPP-SIFNGNVSEMQRIQSEAKHCSG 75

Query: 77  PPHHPSPTVAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLYANDVDLVKDLSLSKNPD 136
                   ++HDY S+LFP+F  WRKQYG +YTYSTG +QHLY N  ++VK+LS +   +
Sbjct: 76  -----DNIISHDYSSSLFPHFDHWRKQYGRIYTYSTGLKQHLYINHPEMVKELSQTNTLN 135

Query: 137 LDKPSYIFKKFAPLLGYGVILSNGADWTLQHKIIAPEFFMDRVRGMAALMVEAAAPMLEK 196
           L + ++I K+  P+LG G+I SNG  W  Q +IIA EF  D+++GM  LMVE+A PML K
Sbjct: 136 LGRITHITKRLNPILGNGIITSNGPHWAHQRRIIAYEFTHDKIKGMVGLMVESAMPMLNK 195

Query: 197 WEGRIGEEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGKQIFSKLRDLQKLISDGN 256
           WE  +   GE    +I V+E L+  SADVI++ACFGSS+ KGK IFS +RDL   I+  +
Sbjct: 196 WEEMVKRGGE-MGCDIRVDEDLKDVSADVIAKACFGSSFSKGKAIFSMIRDLLTAITKRS 255

Query: 257 PLFR-SSFSDRFFQPSKHR--RIKKLEKEIESLIWETVQQRQEECSKTSSSEKDLLQVLM 316
            LFR + F+D  F   KH    I  LE E+ES IWETV++R+ EC  T   +KDL+Q+++
Sbjct: 256 VLFRFNGFTDMVFGSKKHGDVDIDALEMELESSIWETVKEREIECKDT--HKKDLMQLIL 315

Query: 317 EGAMNDPTIGACNESC-KKFIVDNCKTMYFAGHESSAATATWALMLLALHPEWQDRIRSE 376
           EGAM        ++S  ++F+VDNCK++YFAGH+S+A + +W LMLLAL+P WQ +IR E
Sbjct: 316 EGAMRSCDGNLWDKSAYRRFVVDNCKSIYFAGHDSTAVSVSWCLMLLALNPSWQVKIRDE 375

Query: 377 FTQACPDGRLDTTAISQLKSLSMVVQETLRLYPPGAYVARETFAETRLGNVVVPKGVCLW 436
              +C +G  D  +I  LK+++MV+QET+RLYPP   V RE   + RLG++VVPKGVC+W
Sbjct: 376 ILSSCKNGIPDAESIPNLKTVTMVIQETMRLYPPAPIVGREASKDIRLGDLVVPKGVCIW 435

Query: 437 TLIPMLHTEVEIWGEDANEFKPERFANGIAKACKFPQAYLPFGAGHRTCLGKNFALVQLK 496
           TLIP LH + EIWG DAN+FKPERF+ GI+KACK+PQ+Y+PFG G RTC+GKNF ++++K
Sbjct: 436 TLIPALHRDPEIWGPDANDFKPERFSEGISKACKYPQSYIPFGLGPRTCVGKNFGMMEVK 495

Query: 497 IIISLVVSKFRFSLSPEYRHSPSYRIIVEPDDGVKI 529
           +++SL+VSKF F+LSP Y+HSPS++++VEP  GV I
Sbjct: 496 VLVSLIVSKFSFTLSPTYQHSPSHKLLVEPQHGVVI 522

BLAST of Spg028575 vs. TAIR 10
Match: AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2 )

HSP 1 Score: 324.7 bits (831), Expect = 1.4e-88
Identity = 178/499 (35.67%), Postives = 284/499 (56.91%), Query Frame = 0

Query: 39  VRRKLGAQGVTGPPPSSALFGNLPEMKEIELQAAAMASPPHHPSPTVAHDYISTLFPYFV 98
           +++ +  QG+TGP P   L GN+ ++ ++   +A+      +   ++ H+ +  L P++V
Sbjct: 36  IKKFMERQGITGPKP-RLLTGNIIDISKMLSHSAS------NDCSSIHHNIVPRLLPHYV 95

Query: 99  KWRKQYGPLYTYSTGTRQHLYANDVDLVKDLSLSKNPDLDKPSYIFKKFAPLLGYGVILS 158
            W KQYG  +    GT   L   + +++K+L    NP   K     +     +G G++++
Sbjct: 96  SWSKQYGKRFIMWNGTEPRLCLTETEMIKELLTKHNPVTGKSWLQQQGTKGFIGRGLLMA 155

Query: 159 NGADWTLQHKIIAPEFFMDRVRGMAALMVEAAAPMLEKWEGRIGEEGEGAAAEIEVEEGL 218
           NG  W  Q  + AP F  DR++G A  MVE    M E+    +GE       E+E+ E +
Sbjct: 156 NGEAWHHQRHMAAPAFTRDRLKGYAKHMVECTKMMAERLRKEVGE-------EVEIGEEM 215

Query: 219 RGFSADVISRACFGSSYEKGKQIFSKLRDLQKLISDGNPLFRSSFSDRFFQPSKH-RRIK 278
           R  +AD+ISR  FGSS +KGK++FS L  LQ+L +         F    F PSK+ R IK
Sbjct: 216 RRLTADIISRTEFGSSCDKGKELFSLLTVLQRLCAQATR--HLCFPGSRFLPSKYNREIK 275

Query: 279 KLEKEIESLIWETVQQRQE--ECSKTSSSEKDLLQVLMEGAMNDPTIGACNESCKKFIVD 338
            L+ E+E L+ E +  R++  E  ++SS   DLL +L+    ++      N    + I+D
Sbjct: 276 SLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGLLLNQMDSNK-----NNLNVQMIMD 335

Query: 339 NCKTMYFAGHESSAATATWALMLLALHPEWQDRIRSEFTQAC-PDGRLDTTAISQLKSLS 398
            CKT +F GHE+++   TW LMLLA +P WQD +R E  Q C  DG      +S L SL+
Sbjct: 336 ECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDEVRQVCGQDGVPSVEQLSSLTSLN 395

Query: 399 MVVQETLRLYPPGAYVARETFAETRLGNVVVPKGVCLWTLIPMLHTEVEIWGEDANEFKP 458
            V+ E+LRLYPP   + R  F + +LG++++PKG+ +W  +  +H   E+WGEDANEF P
Sbjct: 396 KVINESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSIWIPVLAIHHSNELWGEDANEFNP 455

Query: 459 ERFANGIAKACKFPQAYLPFGAGHRTCLGKNFALVQLKIIISLVVSKFRFSLSPEYRHSP 518
           ERF     ++    + ++PF AG R C+G+ FA+++ KII++++VSKF F++S  YRH+P
Sbjct: 456 ERFT---TRSFASSRHFMPFAAGPRNCIGQTFAMMEAKIILAMLVSKFSFAISENYRHAP 510

Query: 519 SYRIIVEPDDGVKIVFQRL 534
              + ++P  GV++V + L
Sbjct: 516 IVVLTIKPKYGVQLVLKPL 510

BLAST of Spg028575 vs. TAIR 10
Match: AT5G38450.1 (cytochrome P450, family 735, subfamily A, polypeptide 1 )

HSP 1 Score: 320.5 bits (820), Expect = 2.6e-87
Identity = 184/533 (34.52%), Postives = 297/533 (55.72%), Query Frame = 0

Query: 5   LLDGLVSIFPAGVAMAVVAVLVHFYYGVWRRTAEVRRKLGAQGVTGPPPSSALFGNLPEM 64
           +L  L+ IF       ++ VL       W     +++ +  QGVTGP P   L GN+ E+
Sbjct: 5   ILKSLLVIF----VTTILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKP-RPLTGNILEI 64

Query: 65  KEIELQAAAMASPPHHPSPTVAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLYANDVD 124
             +  Q+A+      H      HD +  L P++V W KQYG  +    GT   L   + +
Sbjct: 65  SAMVSQSASKDCDSIH------HDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETE 124

Query: 125 LVKDLSLSKNPDLDKPSYIFKKFAPLLGYGVILSNGADWTLQHKIIAPEFFMDRVRGMAA 184
           L+K+L +  N    +     +     +G G++++NG DW  Q  + AP F  +R++G A 
Sbjct: 125 LIKELLMKHNGVSGRSWLQQQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYAR 184

Query: 185 LMVEAAAPMLEKWEGRIGEEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGKQIFSK 244
            MVE  + ++E+    +GE     A E+E+ E +   +AD+ISR  FGSS+EKGK++F+ 
Sbjct: 185 HMVECTSKLVERLRKEVGE----GANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNH 244

Query: 245 LRDLQKLISDGNPLFRSSFSDRFFQPSKH-RRIKKLEKEIESLIWETVQQRQE--ECSKT 304
           L  LQ+  +         F    F PSK+ R IK L+KE+E L+ E +Q R++  E  ++
Sbjct: 245 LTVLQRRCAQATR--HLCFPGSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRS 304

Query: 305 SSSEKDLLQVLMEGAMNDPTIGACNESCKKFIVDNCKTMYFAGHESSAATATWALMLLAL 364
           S+   DLL +L+   M+       N +  + I+D CKT +FAGHE++A   TW  MLLA 
Sbjct: 305 STHGDDLLGLLL-NEMDIDKNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLAD 364

Query: 365 HPEWQDRIRSEFTQAC-PDGRLDTTAISQLKSLSMVVQETLRLYPPGAYVARETFAETRL 424
           +P WQ+++R E  +    +G      +S+L SLS V+ E+LRLYPP   + R  F + +L
Sbjct: 365 NPTWQEKVREEVREVFGRNGLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKL 424

Query: 425 GNVVVPKGVCLWTLIPMLHTEVEIWGEDANEFKPERFANGIAKACKFPQAYLPFGAGHRT 484
           G++ +PKG+ +W  +  +H   E+WG+DAN+F PERF     +     + ++PF AG R 
Sbjct: 425 GDLTIPKGLSIWIPVLAIHHSEELWGKDANQFNPERFG---GRPFASGRHFIPFAAGPRN 484

Query: 485 CLGKNFALVQLKIIISLVVSKFRFSLSPEYRHSPSYRIIVEPDDGVKIVFQRL 534
           C+G+ FAL++ KII++ ++SKF F++S  YRH+P   + ++P  GV+++ + L
Sbjct: 485 CIGQQFALMEAKIILATLISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPL 516

BLAST of Spg028575 vs. TAIR 10
Match: AT5G52400.1 (cytochrome P450, family 715, subfamily A, polypeptide 1 )

HSP 1 Score: 310.5 bits (794), Expect = 2.7e-84
Identity = 166/523 (31.74%), Postives = 272/523 (52.01%), Query Frame = 0

Query: 12  IFPAGVAMAVVAVLVHFYYGVWRRTAEVRRKLGAQGVTGPPPSSALFGNLPEMKEIELQA 71
           IF     + +  + +  +   W      ++KL   G  GP PS   FGNL +MK++++  
Sbjct: 12  IFFKVFVVVICLMFLKLFLRCWIWPVRAQKKLRGNGFVGPAPSFP-FGNLNDMKKLKM-- 71

Query: 72  AAMASPPHHPSPTVAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLYANDVDLVKDLSL 131
           A++       S  + HD  S   P+F +W+++YG ++ Y  G    +Y  D + +  +S 
Sbjct: 72  ASVVVDNSKSSTIINHDIHSIALPHFARWQQEYGKVFVYWLGIEPFVYVADPEFLSVMSK 131

Query: 132 S-KNPDLDKPSYIFKKFAPLLGYGVILSNGADWTLQHKIIAPEFFMDRVRGMAALMVEAA 191
                   KP+   K   P+ G G+++  G DWT    II P F    ++ M  +MVE+ 
Sbjct: 132 GVLGKSWGKPNVFKKDREPMFGTGLVMVEGDDWTRHRHIITPAFAPLNLKVMTNMMVESV 191

Query: 192 APMLEKWEGRIGEEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGKQIFSKLRDLQK 251
           + ML++W    G +      E ++E  + G + ++I++  FG + E G Q+   LR +Q 
Sbjct: 192 SNMLDRW----GIQINSGNPEFDMESEIIGTAGEIIAKTSFGVTGENGTQVLKNLRAVQF 251

Query: 252 LISDGNPLFRSSFSDRFFQPSKHRRIKKLEKEIESLIWETVQQRQEECSKTSSSEKDLLQ 311
            + + N      FS+      +  + K L  EI+ L+   + +R+   ++      DLL 
Sbjct: 252 ALFNSNRYVGVPFSN-ILSYKQTVKAKGLGHEIDGLLLSFINKRKISLAEGDDQGHDLLG 311

Query: 312 VLMEGAMNDPTIGACNESCKKFIVDNCKTMYFAGHESSAATATWALMLLALHPEWQDRIR 371
           +L++                K +VD CKT +FAGHE++A   TW  MLLA+HPEWQD IR
Sbjct: 312 MLLKADQK-------GNFTAKELVDECKTFFFAGHETTALALTWTFMLLAIHPEWQDTIR 371

Query: 372 SEFTQACPDGRLDTTAISQLKSLSMVVQETLRLYPPGAYVARETFAETRLGNVVVPKGVC 431
            E  +   D +++   ++ LK +S V+ E LRLYPP     R+   +  +   V+P G  
Sbjct: 372 EEIREVIGDSKIEYNKLAGLKKMSWVMNEVLRLYPPAPNAQRQARNDIEVNGRVIPNGTN 431

Query: 432 LWTLIPMLHTEVEIWGEDANEFKPERFANGIAKACKFPQAYLPFGAGHRTCLGKNFALVQ 491
           +W  +  +H +VE+WG+D NEFKPERF   +   CK    Y+PFG G R C+G+N   ++
Sbjct: 432 IWIDVVAMHHDVELWGDDVNEFKPERFDGNLHGGCKNKMGYMPFGFGGRMCIGRNLTTME 491

Query: 492 LKIIISLVVSKFRFSLSPEYRHSPSYRIIVEPDDGVKIVFQRL 534
            KI++SLV+S+F  S+SP YRHSP+Y + + P  G+ ++ + L
Sbjct: 492 YKIVLSLVLSRFEISVSPGYRHSPTYMLSLRPGYGLPLIIRPL 519