Spg028540 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg028540 vs. NCBI nr
Match: KAG7031525.1 (Phosphoglucan phosphatase LSF2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 493.8 bits (1270), Expect = 9.4e-136
Identity = 242/270 (89.63%), Postives = 250/270 (92.59%), Query Frame = 0

Query: 3   MGVFSLDRLSSSLFAVSPLQNHAHNKSLPFCSKLSNPHFRLNKIWCGMPESGIRQNPTSG 62
           MG  SL    SSLFAV P  N+AHNK LPFCS  S P FRLNKI CG+PESGIRQNP+S 
Sbjct: 1   MGASSL-ACFSSLFAVFPSHNNAHNKPLPFCSNPSKPLFRLNKIRCGLPESGIRQNPSSS 60

Query: 63  RASTSRNRMEEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEE 122
           RAS+S+NRMEEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEE
Sbjct: 61  RASSSKNRMEEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEE 120

Query: 123 GVAYILNLQQDKDVEYWGINLQSIIERCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLE 182
           GVAYILNLQQDKDVEYWGI+LQS+IERCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLE
Sbjct: 121 GVAYILNLQQDKDVEYWGIDLQSVIERCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLE 180

Query: 183 WAISEGKGKVYVHCTAGLGRAPALNTAYEALTSKRPCGPSKKAIRGATYDLSKNDPWKEP 242
           WAISEGKGK YVHCTAGLGRAPALNTAYEALTSKRPCGPSKKAIRGATYDLSKNDP KEP
Sbjct: 181 WAISEGKGKAYVHCTAGLGRAPALNTAYEALTSKRPCGPSKKAIRGATYDLSKNDPGKEP 240

Query: 243 FESLPDNAFEGIADWERNLIQDRIRSLRGT 273
           FESLPDNAFE IADWERNLIQDR+RSLR T
Sbjct: 241 FESLPDNAFEDIADWERNLIQDRVRSLRRT 269

BLAST of Spg028540 vs. NCBI nr
Match: XP_022942267.1 (phosphoglucan phosphatase LSF2, chloroplastic [Cucurbita moschata])

HSP 1 Score: 486.5 bits (1251), Expect = 1.5e-133
Identity = 243/283 (85.87%), Postives = 251/283 (88.69%), Query Frame = 0

Query: 3   MGVFSLDRLSSSLFAVSPLQNHAHNKSLPFCSKLSNPHFRLNKIWCGMPESGIRQNPTSG 62
           MG  SL    SSLFAV P  N+AHNKSLPFCS  S P FRLNKI CG+PESGIRQNP+S 
Sbjct: 1   MGASSL-ACFSSLFAVFPSHNNAHNKSLPFCSNPSKPLFRLNKIRCGLPESGIRQNPSSS 60

Query: 63  RASTSRNRMEEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEE 122
           RAS+S+NRMEEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEE
Sbjct: 61  RASSSKNRMEEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEE 120

Query: 123 GVAYILNLQQDKDVEYWGINLQSIIERCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLE 182
           GVAYILNLQQDKDVEYWGI+LQS+IERCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLE
Sbjct: 121 GVAYILNLQQDKDVEYWGIDLQSVIERCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLE 180

Query: 183 WAISEGKGKVYVHCTAGLGRAPA-------------LNTAYEALTSKRPCGPSKKAIRGA 242
           WAISEGKGK YVHCTAGLGRAPA             LNTAYEALTSKRPCGPSKKAIRGA
Sbjct: 181 WAISEGKGKAYVHCTAGLGRAPAVAIAYLYWFCGMNLNTAYEALTSKRPCGPSKKAIRGA 240

Query: 243 TYDLSKNDPWKEPFESLPDNAFEGIADWERNLIQDRIRSLRGT 273
           TYDLSKNDP KEPFESLPDNAFE IADWERNLIQDR+RSLR T
Sbjct: 241 TYDLSKNDPGKEPFESLPDNAFEDIADWERNLIQDRVRSLRRT 282

BLAST of Spg028540 vs. NCBI nr
Match: XP_023542341.1 (phosphoglucan phosphatase LSF2, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 484.2 bits (1245), Expect = 7.5e-133
Identity = 242/283 (85.51%), Postives = 250/283 (88.34%), Query Frame = 0

Query: 3   MGVFSLDRLSSSLFAVSPLQNHAHNKSLPFCSKLSNPHFRLNKIWCGMPESGIRQNPTSG 62
           MG  SL    SSLFAV P  N+AHNKSLPFCS  S P FRLNKI CG+PESGIRQ P+S 
Sbjct: 1   MGASSL-ACFSSLFAVFPSHNNAHNKSLPFCSNPSKPLFRLNKIRCGLPESGIRQTPSSS 60

Query: 63  RASTSRNRMEEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEE 122
           RAS+S+NRMEEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEE
Sbjct: 61  RASSSKNRMEEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEE 120

Query: 123 GVAYILNLQQDKDVEYWGINLQSIIERCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLE 182
           GVAYILNLQQDKDVEYWGI+LQS+IERCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLE
Sbjct: 121 GVAYILNLQQDKDVEYWGIDLQSVIERCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLE 180

Query: 183 WAISEGKGKVYVHCTAGLGRAPA-------------LNTAYEALTSKRPCGPSKKAIRGA 242
           WAISEGKGK YVHCTAGLGRAPA             LNTAYEALTSKRPCGPSKKAIRGA
Sbjct: 181 WAISEGKGKAYVHCTAGLGRAPAVAIAYLYWFCGMNLNTAYEALTSKRPCGPSKKAIRGA 240

Query: 243 TYDLSKNDPWKEPFESLPDNAFEGIADWERNLIQDRIRSLRGT 273
           TYDLSKNDP KEPFESLPDNAFE IADWERNLIQDR+RSLR T
Sbjct: 241 TYDLSKNDPGKEPFESLPDNAFEDIADWERNLIQDRVRSLRRT 282

BLAST of Spg028540 vs. NCBI nr
Match: XP_022984858.1 (phosphoglucan phosphatase LSF2, chloroplastic [Cucurbita maxima])

HSP 1 Score: 483.8 bits (1244), Expect = 9.7e-133
Identity = 241/283 (85.16%), Postives = 251/283 (88.69%), Query Frame = 0

Query: 3   MGVFSLDRLSSSLFAVSPLQNHAHNKSLPFCSKLSNPHFRLNKIWCGMPESGIRQNPTSG 62
           MG  SL    SSLFAV P  N+AHNKSLPFCS  S P FRLNKI CG+PESGIRQNP+S 
Sbjct: 1   MGASSL-ACFSSLFAVFPSHNNAHNKSLPFCSNPSKPLFRLNKIRCGLPESGIRQNPSSS 60

Query: 63  RASTSRNRMEEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEE 122
           R S+S+NRMEEYNLAMKKMMRNPYEYHHELGMNYT+IADNLIVGSQPQKPEDIDFLKEEE
Sbjct: 61  RTSSSKNRMEEYNLAMKKMMRNPYEYHHELGMNYTLIADNLIVGSQPQKPEDIDFLKEEE 120

Query: 123 GVAYILNLQQDKDVEYWGINLQSIIERCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLE 182
           GVAYILNLQQDKDVEYWGI+LQS+IERCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLE
Sbjct: 121 GVAYILNLQQDKDVEYWGIDLQSVIERCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLE 180

Query: 183 WAISEGKGKVYVHCTAGLGRAPA-------------LNTAYEALTSKRPCGPSKKAIRGA 242
           WAISEGKGKVYVHCTAGLGRAPA             LNTAYEALTSKRPCGPSKKAIRGA
Sbjct: 181 WAISEGKGKVYVHCTAGLGRAPAVAIAYLYWFCGMNLNTAYEALTSKRPCGPSKKAIRGA 240

Query: 243 TYDLSKNDPWKEPFESLPDNAFEGIADWERNLIQDRIRSLRGT 273
           TYDLSKNDP KEPFESLPDNAFE IADWERNLIQDR+RSL+ T
Sbjct: 241 TYDLSKNDPGKEPFESLPDNAFEDIADWERNLIQDRVRSLQRT 282

BLAST of Spg028540 vs. NCBI nr
Match: XP_038891555.1 (phosphoglucan phosphatase LSF2, chloroplastic [Benincasa hispida])

HSP 1 Score: 469.2 bits (1206), Expect = 2.5e-128
Identity = 230/273 (84.25%), Postives = 244/273 (89.38%), Query Frame = 0

Query: 13  SSLFAVSPLQNHAHNKSLPFCSKLSNPHFRLNKIWCGMPESGIRQNPTSGRASTSRNRME 72
           S LFA  P QN+A NK LPF S +S PHFRLN+I CG PESGI+Q P++ RAS+S+NRME
Sbjct: 53  SLLFAFFPHQNNAPNKFLPFSSIISKPHFRLNRICCGAPESGIQQIPSTSRASSSKNRME 112

Query: 73  EYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEEGVAYILNLQQ 132
           EYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDI+FLKEEEGVAYILNLQQ
Sbjct: 113 EYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDINFLKEEEGVAYILNLQQ 172

Query: 133 DKDVEYWGINLQSIIERCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLEWAISEGKGKV 192
           DKDVEYWGI+LQSII+RCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLEWAISEGKGKV
Sbjct: 173 DKDVEYWGIDLQSIIKRCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLEWAISEGKGKV 232

Query: 193 YVHCTAGLGRAPA-------------LNTAYEALTSKRPCGPSKKAIRGATYDLSKNDPW 252
           YVHCTAGLGRAPA             LNTAYEALTSKRPCGPSK+AIRGATYDLSKNDPW
Sbjct: 233 YVHCTAGLGRAPAVAIAYLYWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLSKNDPW 292

Query: 253 KEPFESLPDNAFEGIADWERNLIQDRIRSLRGT 273
           KEPFESLPDNAFE IADWERNLI+DR+ SLR T
Sbjct: 293 KEPFESLPDNAFEDIADWERNLIRDRVHSLRRT 325

BLAST of Spg028540 vs. ExPASy Swiss-Prot
Match: Q9SRK5 (Phosphoglucan phosphatase LSF2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LSF2 PE=1 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 2.4e-97
Identity = 179/276 (64.86%), Postives = 215/276 (77.90%), Query Frame = 0

Query: 13  SSLFAVSPLQNHAHNKSLPFCS--KLSNPHFRLNKIWCGMPESGIRQNP-TSGRASTSRN 72
           S +F+V+      + KS  F S  K S+   R  +   G+      +NP T+G + +S+N
Sbjct: 8   SCIFSVA-RYTRENEKSSCFTSINKKSSLDLRFPRNLAGVSCKFSGENPGTNGVSLSSKN 67

Query: 73  RMEEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEEGVAYILN 132
           +ME+YN AMK++MR+PYEYHH+LGMNYT+I D LIVGSQPQKPEDID LK+E+ VAYILN
Sbjct: 68  KMEDYNTAMKRLMRSPYEYHHDLGMNYTLIRDELIVGSQPQKPEDIDHLKQEQNVAYILN 127

Query: 133 LQQDKDVEYWGINLQSIIERCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLEWAISEGK 192
           LQQDKD+EYWGI+L SI+ RCKELGI HMR+PAKDFDP SLR+ LPKAVS LEWA+SEGK
Sbjct: 128 LQQDKDIEYWGIDLDSIVRRCKELGIRHMRRPAKDFDPLSLRSQLPKAVSSLEWAVSEGK 187

Query: 193 GKVYVHCTAGLGRAPA-------------LNTAYEALTSKRPCGPSKKAIRGATYDLSKN 252
           G+VYVHC+AGLGRAP              LNTAY+ L SKRPCGP+K AIRGATYDL+KN
Sbjct: 188 GRVYVHCSAGLGRAPGVSIAYMYWFCDMNLNTAYDTLVSKRPCGPNKGAIRGATYDLAKN 247

Query: 253 DPWKEPFESLPDNAFEGIADWERNLIQDRIRSLRGT 273
           DPWKEPFESLP+NAFE IADWER LIQ+R+R+LRGT
Sbjct: 248 DPWKEPFESLPENAFEDIADWERKLIQERVRALRGT 282

BLAST of Spg028540 vs. ExPASy Swiss-Prot
Match: Q9FEB5 (Phosphoglucan phosphatase DSP4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DSP4 PE=1 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 1.2e-29
Identity = 93/260 (35.77%), Postives = 125/260 (48.08%), Query Frame = 0

Query: 17  AVSPLQNHA-----HNKSLPFCSKLSNPHFRLNKIWCGMPESGIRQNPTSGRAS---TSR 76
           +VSPL         H+ S      L +P  + N     +    + ++ +S   S      
Sbjct: 11  SVSPLLGFGCIQRDHSSSSSSLKMLISPPIKANDPKSRLVLHAVSESKSSSEMSGVAKDE 70

Query: 77  NRMEEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEEGVAYIL 136
            + +EY+  M + M     Y HELGMNY  I  +LIVGS  Q PED+D L+ + GV  I 
Sbjct: 71  EKSDEYSQDMTQAMGAVLTYRHELGMNYNFIRPDLIVGSCLQTPEDVDKLR-KIGVKTIF 130

Query: 137 NLQQDKDVEYWGINLQSIIERCKELG-IHHMRQPAKDFDPDSLRNGLPKAVSLLEWAISE 196
            LQQD D+EY+G+++ SI    K+   I H+R   +DFD   LR  LP  V  L  A+  
Sbjct: 131 CLQQDPDLEYFGVDISSIQAYAKKYSDIQHIRCEIRDFDAFDLRMRLPAVVGTLYKAVKR 190

Query: 197 GKGKVYVHCTAGLGRAPA-------------LNTAYEALTSKRPCGPSKKAIRGATYDLS 255
             G  YVHCTAG+GRAPA             L  A++ L SKR C P   AIR AT D+ 
Sbjct: 191 NGGVTYVHCTAGMGRAPAVALTYMFWVQGYKLMEAHKLLMSKRSCFPKLDAIRNATIDIL 250

BLAST of Spg028540 vs. ExPASy Swiss-Prot
Match: G4LTX4 (Phosphoglucan phosphatase DSP4, amyloplastic OS=Castanea sativa OX=21020 GN=DSP4 PE=1 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 2.7e-29
Identity = 89/233 (38.20%), Postives = 117/233 (50.21%), Query Frame = 0

Query: 20  PLQNHAHNKSLPFCS-----KLSNPHFRLNKIWCGMPESGIRQNPTSGRASTSRNRMEEY 79
           PLQ+   ++  P CS      +SN    L++       SG   +  +  A+    + E Y
Sbjct: 14  PLQSFKSHQRRPPCSVNTLGVMSN--VNLHRSMAVKAISGPTSSAETSDANVEEEKSETY 73

Query: 80  NLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEEGVAYILNLQQDK 139
           +  M + M     Y HELG+NY  I  +LIVGS  Q PED+D L+   GV  I  LQQ+ 
Sbjct: 74  STNMTEAMGAVLTYRHELGINYNFIRPDLIVGSCLQTPEDVDKLR-SIGVKTIFCLQQNS 133

Query: 140 DVEYWGINLQSIIERCKEL-GIHHMRQPAKDFDPDSLRNGLPKAVSLLEWAISEGKGKVY 199
           D+EY+G+++  I E  K    I H+R   +DFD   LR  LP  VS L  AI+   G  Y
Sbjct: 134 DLEYFGVDINGIREYAKTYDDIQHLRAEIRDFDAFDLRVRLPAVVSKLYKAINRNGGVTY 193

Query: 200 VHCTAGLGRAPALNTAY-------------EALTSKRPCGPSKKAIRGATYDL 234
           VHCTAGLGRAPA+  AY               L SKR C P   AI+ A  D+
Sbjct: 194 VHCTAGLGRAPAVTLAYMFWVQGYKLIEAHNLLQSKRSCFPKLDAIKSAAADI 243

BLAST of Spg028540 vs. ExPASy Swiss-Prot
Match: F4J117 (Phosphoglucan phosphatase LSF1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LSF1 PE=1 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 5.7e-19
Identity = 63/188 (33.51%), Postives = 91/188 (48.40%), Query Frame = 0

Query: 59  PTSGRASTSRNRMEEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFL 118
           P  G        +EEY  A+ +  +    Y+H LGM Y+ I + + VGS  Q  ED++ L
Sbjct: 256 PGDGEWGYGNFPLEEYIKALDR-SKGELSYNHALGMRYSKITEQIYVGSCIQTEEDVENL 315

Query: 119 KEEEGVAYILNLQQDKDVEYWGINLQSIIERCKELGIHHMRQPAKDFDPDSLRNGLPKAV 178
             E G+  ILN Q   + + WGI+ QSI + C++  +  +  P KD D   LR  LP  V
Sbjct: 316 -SEAGITAILNFQGGTEAQNWGIDSQSINDACQKSEVLMINYPIKDADSFDLRKKLPLCV 375

Query: 179 SLLEWAISEGKGKVYVHCTAGLGRAPA-------------LNTAYEALTSKRPCGPSKKA 234
            LL   + +   +V+V CT G  R+ A             L+ AY  +T    C P + A
Sbjct: 376 GLLLRLLKKNH-RVFVTCTTGFDRSSACVIAYLHWMTDTSLHAAYSFVTGLHACKPDRPA 435

BLAST of Spg028540 vs. ExPASy Swiss-Prot
Match: Q1M199 (Laforin OS=Canis lupus familiaris OX=9615 GN=EPM2A PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 2.0e-08
Identity = 42/122 (34.43%), Postives = 64/122 (52.46%), Query Frame = 0

Query: 94  MNYTVIADNLIVGSQPQKPEDIDF-LKEEEGVAYILNLQQDKDV--EYWGINL------- 153
           M+Y+ I  N+ +GS P++ E I   LK E G+  ++N Q + D+     G N        
Sbjct: 155 MHYSRILPNIWLGSCPRQVEHITIKLKHELGITAVMNFQTEWDIVQNSSGCNRYPEPMTP 214

Query: 154 QSIIERCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLEWAISEGKGKVYVHCTAGLGRA 206
            ++I+  KE G+ ++  P  D   +     LP+AV LL  A+ E    VYVHC AG+GR+
Sbjct: 215 DTMIKLYKEEGLVYIWMPTPDMSTEGRVQMLPQAVCLLH-ALLENGHTVYVHCNAGVGRS 274

BLAST of Spg028540 vs. ExPASy TrEMBL
Match: A0A6J1FPR7 (phosphoglucan phosphatase LSF2, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111447363 PE=4 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 7.3e-134
Identity = 243/283 (85.87%), Postives = 251/283 (88.69%), Query Frame = 0

Query: 3   MGVFSLDRLSSSLFAVSPLQNHAHNKSLPFCSKLSNPHFRLNKIWCGMPESGIRQNPTSG 62
           MG  SL    SSLFAV P  N+AHNKSLPFCS  S P FRLNKI CG+PESGIRQNP+S 
Sbjct: 1   MGASSL-ACFSSLFAVFPSHNNAHNKSLPFCSNPSKPLFRLNKIRCGLPESGIRQNPSSS 60

Query: 63  RASTSRNRMEEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEE 122
           RAS+S+NRMEEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEE
Sbjct: 61  RASSSKNRMEEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEE 120

Query: 123 GVAYILNLQQDKDVEYWGINLQSIIERCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLE 182
           GVAYILNLQQDKDVEYWGI+LQS+IERCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLE
Sbjct: 121 GVAYILNLQQDKDVEYWGIDLQSVIERCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLE 180

Query: 183 WAISEGKGKVYVHCTAGLGRAPA-------------LNTAYEALTSKRPCGPSKKAIRGA 242
           WAISEGKGK YVHCTAGLGRAPA             LNTAYEALTSKRPCGPSKKAIRGA
Sbjct: 181 WAISEGKGKAYVHCTAGLGRAPAVAIAYLYWFCGMNLNTAYEALTSKRPCGPSKKAIRGA 240

Query: 243 TYDLSKNDPWKEPFESLPDNAFEGIADWERNLIQDRIRSLRGT 273
           TYDLSKNDP KEPFESLPDNAFE IADWERNLIQDR+RSLR T
Sbjct: 241 TYDLSKNDPGKEPFESLPDNAFEDIADWERNLIQDRVRSLRRT 282

BLAST of Spg028540 vs. ExPASy TrEMBL
Match: A0A6J1JBS6 (phosphoglucan phosphatase LSF2, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111483010 PE=4 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 4.7e-133
Identity = 241/283 (85.16%), Postives = 251/283 (88.69%), Query Frame = 0

Query: 3   MGVFSLDRLSSSLFAVSPLQNHAHNKSLPFCSKLSNPHFRLNKIWCGMPESGIRQNPTSG 62
           MG  SL    SSLFAV P  N+AHNKSLPFCS  S P FRLNKI CG+PESGIRQNP+S 
Sbjct: 1   MGASSL-ACFSSLFAVFPSHNNAHNKSLPFCSNPSKPLFRLNKIRCGLPESGIRQNPSSS 60

Query: 63  RASTSRNRMEEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEE 122
           R S+S+NRMEEYNLAMKKMMRNPYEYHHELGMNYT+IADNLIVGSQPQKPEDIDFLKEEE
Sbjct: 61  RTSSSKNRMEEYNLAMKKMMRNPYEYHHELGMNYTLIADNLIVGSQPQKPEDIDFLKEEE 120

Query: 123 GVAYILNLQQDKDVEYWGINLQSIIERCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLE 182
           GVAYILNLQQDKDVEYWGI+LQS+IERCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLE
Sbjct: 121 GVAYILNLQQDKDVEYWGIDLQSVIERCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLE 180

Query: 183 WAISEGKGKVYVHCTAGLGRAPA-------------LNTAYEALTSKRPCGPSKKAIRGA 242
           WAISEGKGKVYVHCTAGLGRAPA             LNTAYEALTSKRPCGPSKKAIRGA
Sbjct: 181 WAISEGKGKVYVHCTAGLGRAPAVAIAYLYWFCGMNLNTAYEALTSKRPCGPSKKAIRGA 240

Query: 243 TYDLSKNDPWKEPFESLPDNAFEGIADWERNLIQDRIRSLRGT 273
           TYDLSKNDP KEPFESLPDNAFE IADWERNLIQDR+RSL+ T
Sbjct: 241 TYDLSKNDPGKEPFESLPDNAFEDIADWERNLIQDRVRSLQRT 282

BLAST of Spg028540 vs. ExPASy TrEMBL
Match: A0A0A0KQM6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G218830 PE=4 SV=1)

HSP 1 Score: 468.0 bits (1203), Expect = 2.7e-128
Identity = 228/273 (83.52%), Postives = 245/273 (89.74%), Query Frame = 0

Query: 13  SSLFAVSPLQNHAHNKSLPFCSKLSNPHFRLNKIWCGMPESGIRQNPTSGRASTSRNRME 72
           SSLF V PLQN+A NK LPF S LS P +R N+I CG+PESGI+QNP++ R S+S+NRME
Sbjct: 10  SSLFGVFPLQNNAPNKFLPFSSILSKPLYRFNRICCGVPESGIQQNPSTSRPSSSKNRME 69

Query: 73  EYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEEGVAYILNLQQ 132
           +YNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDI+FLKEEEGVAYILNLQQ
Sbjct: 70  DYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDINFLKEEEGVAYILNLQQ 129

Query: 133 DKDVEYWGINLQSIIERCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLEWAISEGKGKV 192
           DKDVEYWGI+LQSIIERCKELGIHHMRQPAKDFDPDSLR GLPKAVSLLEWAIS+GKGKV
Sbjct: 130 DKDVEYWGIDLQSIIERCKELGIHHMRQPAKDFDPDSLRTGLPKAVSLLEWAISKGKGKV 189

Query: 193 YVHCTAGLGRAPA-------------LNTAYEALTSKRPCGPSKKAIRGATYDLSKNDPW 252
           YVHCTAGLGRAPA             LNTAYEALTSKRPCGPSK+AIRGATYDLSKNDPW
Sbjct: 190 YVHCTAGLGRAPAVAIAYLYWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLSKNDPW 249

Query: 253 KEPFESLPDNAFEGIADWERNLIQDRIRSLRGT 273
           KEPFESLPDNAFE IADWERNLI+DR+R+LR T
Sbjct: 250 KEPFESLPDNAFEDIADWERNLIRDRVRALRRT 282

BLAST of Spg028540 vs. ExPASy TrEMBL
Match: A0A1S3C4E0 (phosphoglucan phosphatase LSF2, chloroplastic isoform X3 OS=Cucumis melo OX=3656 GN=LOC103496723 PE=4 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 3.5e-128
Identity = 229/273 (83.88%), Postives = 243/273 (89.01%), Query Frame = 0

Query: 13  SSLFAVSPLQNHAHNKSLPFCSKLSNPHFRLNKIWCGMPESGIRQNPTSGRASTSRNRME 72
           SSLF V PLQN+A NK LPF S LS P +R N+I CG+PESGI+QNP++ R S+S+NRM+
Sbjct: 10  SSLFGVFPLQNNAPNKLLPFSSTLSKPLYRFNRICCGVPESGIQQNPSTSRPSSSKNRMD 69

Query: 73  EYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEEGVAYILNLQQ 132
           EYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDI+FLKEEEGVAYILNLQQ
Sbjct: 70  EYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDINFLKEEEGVAYILNLQQ 129

Query: 133 DKDVEYWGINLQSIIERCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLEWAISEGKGKV 192
           DKDVEYWGINLQSIIERCKELGI HMRQPAKDFDPDSLR GLPKAVSLLEWAIS+GKGKV
Sbjct: 130 DKDVEYWGINLQSIIERCKELGIRHMRQPAKDFDPDSLRTGLPKAVSLLEWAISKGKGKV 189

Query: 193 YVHCTAGLGRAPA-------------LNTAYEALTSKRPCGPSKKAIRGATYDLSKNDPW 252
           YVHCTAGLGRAPA             LNTAYEALTSKRPCGPSK+AIRGATYDLSKNDPW
Sbjct: 190 YVHCTAGLGRAPAVAIAYLYWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLSKNDPW 249

Query: 253 KEPFESLPDNAFEGIADWERNLIQDRIRSLRGT 273
           KEPFESLPDNAFE IADWERNLI DR+RSLR T
Sbjct: 250 KEPFESLPDNAFEDIADWERNLIWDRVRSLRRT 282

BLAST of Spg028540 vs. ExPASy TrEMBL
Match: A0A6J1D0C4 (phosphoglucan phosphatase LSF2, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111015894 PE=4 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 7.3e-126
Identity = 221/273 (80.95%), Postives = 239/273 (87.55%), Query Frame = 0

Query: 13  SSLFAVSPLQNHAHNKSLPFCSKLSNPHFRLNKIWCGMPESGIRQNPTSGRASTSRNRME 72
           SSL  + PLQNH  NK L F SKLS PH + ++I CGMPE+G+RQNPTSG ASTSRN  E
Sbjct: 11  SSLSVILPLQNHTPNKFLSFPSKLSKPHLQFHRICCGMPETGVRQNPTSGGASTSRNSTE 70

Query: 73  EYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEEGVAYILNLQQ 132
            YN+ MKKMM+NPYEYHHELGMNYTVIADNLIVGSQPQKP+DIDFLKEEEGVAYILNLQQ
Sbjct: 71  MYNMTMKKMMKNPYEYHHELGMNYTVIADNLIVGSQPQKPKDIDFLKEEEGVAYILNLQQ 130

Query: 133 DKDVEYWGINLQSIIERCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLEWAISEGKGKV 192
           DKDVEYWG++L+SIIERCKELGIHHMR+PA+DFDPDS+RNGLPKAVSLLEWAISEGKGKV
Sbjct: 131 DKDVEYWGVDLRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLEWAISEGKGKV 190

Query: 193 YVHCTAGLGRAPA-------------LNTAYEALTSKRPCGPSKKAIRGATYDLSKNDPW 252
           YVHCTAGLGRAPA             LNTAYEALTSKRPCGPSK+AIRGATYDL K D W
Sbjct: 191 YVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLCKTDQW 250

Query: 253 KEPFESLPDNAFEGIADWERNLIQDRIRSLRGT 273
           KEPFESLPDNAFEG+ADWERNLIQDR+RSLR T
Sbjct: 251 KEPFESLPDNAFEGVADWERNLIQDRVRSLRKT 283

BLAST of Spg028540 vs. TAIR 10
Match: AT3G10940.1 (dual specificity protein phosphatase (DsPTP1) family protein )

HSP 1 Score: 356.7 bits (914), Expect = 1.7e-98
Identity = 179/276 (64.86%), Postives = 215/276 (77.90%), Query Frame = 0

Query: 13  SSLFAVSPLQNHAHNKSLPFCS--KLSNPHFRLNKIWCGMPESGIRQNP-TSGRASTSRN 72
           S +F+V+      + KS  F S  K S+   R  +   G+      +NP T+G + +S+N
Sbjct: 8   SCIFSVA-RYTRENEKSSCFTSINKKSSLDLRFPRNLAGVSCKFSGENPGTNGVSLSSKN 67

Query: 73  RMEEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEEGVAYILN 132
           +ME+YN AMK++MR+PYEYHH+LGMNYT+I D LIVGSQPQKPEDID LK+E+ VAYILN
Sbjct: 68  KMEDYNTAMKRLMRSPYEYHHDLGMNYTLIRDELIVGSQPQKPEDIDHLKQEQNVAYILN 127

Query: 133 LQQDKDVEYWGINLQSIIERCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLEWAISEGK 192
           LQQDKD+EYWGI+L SI+ RCKELGI HMR+PAKDFDP SLR+ LPKAVS LEWA+SEGK
Sbjct: 128 LQQDKDIEYWGIDLDSIVRRCKELGIRHMRRPAKDFDPLSLRSQLPKAVSSLEWAVSEGK 187

Query: 193 GKVYVHCTAGLGRAPA-------------LNTAYEALTSKRPCGPSKKAIRGATYDLSKN 252
           G+VYVHC+AGLGRAP              LNTAY+ L SKRPCGP+K AIRGATYDL+KN
Sbjct: 188 GRVYVHCSAGLGRAPGVSIAYMYWFCDMNLNTAYDTLVSKRPCGPNKGAIRGATYDLAKN 247

Query: 253 DPWKEPFESLPDNAFEGIADWERNLIQDRIRSLRGT 273
           DPWKEPFESLP+NAFE IADWER LIQ+R+R+LRGT
Sbjct: 248 DPWKEPFESLPENAFEDIADWERKLIQERVRALRGT 282

BLAST of Spg028540 vs. TAIR 10
Match: AT3G52180.1 (dual specificity protein phosphatase (DsPTP1) family protein )

HSP 1 Score: 131.7 bits (330), Expect = 8.8e-31
Identity = 93/260 (35.77%), Postives = 125/260 (48.08%), Query Frame = 0

Query: 17  AVSPLQNHA-----HNKSLPFCSKLSNPHFRLNKIWCGMPESGIRQNPTSGRAS---TSR 76
           +VSPL         H+ S      L +P  + N     +    + ++ +S   S      
Sbjct: 11  SVSPLLGFGCIQRDHSSSSSSLKMLISPPIKANDPKSRLVLHAVSESKSSSEMSGVAKDE 70

Query: 77  NRMEEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEEGVAYIL 136
            + +EY+  M + M     Y HELGMNY  I  +LIVGS  Q PED+D L+ + GV  I 
Sbjct: 71  EKSDEYSQDMTQAMGAVLTYRHELGMNYNFIRPDLIVGSCLQTPEDVDKLR-KIGVKTIF 130

Query: 137 NLQQDKDVEYWGINLQSIIERCKELG-IHHMRQPAKDFDPDSLRNGLPKAVSLLEWAISE 196
            LQQD D+EY+G+++ SI    K+   I H+R   +DFD   LR  LP  V  L  A+  
Sbjct: 131 CLQQDPDLEYFGVDISSIQAYAKKYSDIQHIRCEIRDFDAFDLRMRLPAVVGTLYKAVKR 190

Query: 197 GKGKVYVHCTAGLGRAPA-------------LNTAYEALTSKRPCGPSKKAIRGATYDLS 255
             G  YVHCTAG+GRAPA             L  A++ L SKR C P   AIR AT D+ 
Sbjct: 191 NGGVTYVHCTAGMGRAPAVALTYMFWVQGYKLMEAHKLLMSKRSCFPKLDAIRNATIDIL 250

BLAST of Spg028540 vs. TAIR 10
Match: AT3G52180.2 (dual specificity protein phosphatase (DsPTP1) family protein )

HSP 1 Score: 131.7 bits (330), Expect = 8.8e-31
Identity = 93/260 (35.77%), Postives = 125/260 (48.08%), Query Frame = 0

Query: 17  AVSPLQNHA-----HNKSLPFCSKLSNPHFRLNKIWCGMPESGIRQNPTSGRAS---TSR 76
           +VSPL         H+ S      L +P  + N     +    + ++ +S   S      
Sbjct: 11  SVSPLLGFGCIQRDHSSSSSSLKMLISPPIKANDPKSRLVLHAVSESKSSSEMSGVAKDE 70

Query: 77  NRMEEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEEGVAYIL 136
            + +EY+  M + M     Y HELGMNY  I  +LIVGS  Q PED+D L+ + GV  I 
Sbjct: 71  EKSDEYSQDMTQAMGAVLTYRHELGMNYNFIRPDLIVGSCLQTPEDVDKLR-KIGVKTIF 130

Query: 137 NLQQDKDVEYWGINLQSIIERCKELG-IHHMRQPAKDFDPDSLRNGLPKAVSLLEWAISE 196
            LQQD D+EY+G+++ SI    K+   I H+R   +DFD   LR  LP  V  L  A+  
Sbjct: 131 CLQQDPDLEYFGVDISSIQAYAKKYSDIQHIRCEIRDFDAFDLRMRLPAVVGTLYKAVKR 190

Query: 197 GKGKVYVHCTAGLGRAPA-------------LNTAYEALTSKRPCGPSKKAIRGATYDLS 255
             G  YVHCTAG+GRAPA             L  A++ L SKR C P   AIR AT D+ 
Sbjct: 191 NGGVTYVHCTAGMGRAPAVALTYMFWVQGYKLMEAHKLLMSKRSCFPKLDAIRNATIDIL 250

BLAST of Spg028540 vs. TAIR 10
Match: AT3G01510.1 (like SEX4 1 )

HSP 1 Score: 96.3 bits (238), Expect = 4.1e-20
Identity = 63/188 (33.51%), Postives = 91/188 (48.40%), Query Frame = 0

Query: 59  PTSGRASTSRNRMEEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFL 118
           P  G        +EEY  A+ +  +    Y+H LGM Y+ I + + VGS  Q  ED++ L
Sbjct: 256 PGDGEWGYGNFPLEEYIKALDR-SKGELSYNHALGMRYSKITEQIYVGSCIQTEEDVENL 315

Query: 119 KEEEGVAYILNLQQDKDVEYWGINLQSIIERCKELGIHHMRQPAKDFDPDSLRNGLPKAV 178
             E G+  ILN Q   + + WGI+ QSI + C++  +  +  P KD D   LR  LP  V
Sbjct: 316 -SEAGITAILNFQGGTEAQNWGIDSQSINDACQKSEVLMINYPIKDADSFDLRKKLPLCV 375

Query: 179 SLLEWAISEGKGKVYVHCTAGLGRAPA-------------LNTAYEALTSKRPCGPSKKA 234
            LL   + +   +V+V CT G  R+ A             L+ AY  +T    C P + A
Sbjct: 376 GLLLRLLKKNH-RVFVTCTTGFDRSSACVIAYLHWMTDTSLHAAYSFVTGLHACKPDRPA 435