Homology
BLAST of Spg028496 vs. NCBI nr
Match:
XP_038892632.1 (tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Benincasa hispida])
HSP 1 Score: 791.6 bits (2043), Expect = 3.5e-225
Identity = 389/440 (88.41%), Postives = 409/440 (92.95%), Query Frame = 0
Query: 1 MLTVTPKTLSPSPFLHSPQTNSHCLTSSTIHLFRSSKWMKKSLNNCLSNNRTERGVEFDI 60
ML+V P+T SPSPFLHSPQTNSH L++STIHLF+ S K SLNNCL NNRTERG+EFDI
Sbjct: 1 MLSVLPRTPSPSPFLHSPQTNSHSLSASTIHLFQFSHSNKNSLNNCLPNNRTERGLEFDI 60
Query: 61 GDTFFRHESATGRDLGVLAAALYRKSKGSIRVLDALCGCGIRSLRYLVEAEADFLWANDA 120
G TFFRHESATGRDLGVLAAALYRKSKG +RVLDALCGCGIRSLRYLVEAEADF+ ANDA
Sbjct: 61 GGTFFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120
Query: 121 NDENRRVIVKNLSRVGRGFGDDRRWVVTNCDANRVMTEIYLHKDFFDFIDVDSFGSDSTF 180
NDENRRVIVKNLSRV GD RRW+VTNCDANRVMT YL KDFFDFID+DSFGSDS+F
Sbjct: 121 NDENRRVIVKNLSRVAEDSGDARRWMVTNCDANRVMTGFYLQKDFFDFIDIDSFGSDSSF 180
Query: 181 LRSAINALKLGGLLYVTSTDGFSSGGHRPSQSLGAYGAYVRPMPFSNEIGLRMLIGGVVR 240
LR AINALKL GLLYVTSTDGFSSGGHRPSQSL AYGAYVRPMPFSNEIGLRMLIGGV R
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLQINRGKIYKDRHYGFISYCEKCGISQAFSWSEL 300
EASVLG+YATPLFSYYSYHGPVFRVML+INRG++YK+RHYGFISYCEKCG SQAFSWSEL
Sbjct: 241 EASVLGHYATPLFSYYSYHGPVFRVMLRINRGRVYKNRHYGFISYCEKCGNSQAFSWSEL 300
Query: 301 GQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEEMLSLAKQWEWIGNGQGKDLEKLLRQ 360
GQMSCPCNDSRVS+SLVVSGPLWTGPLHSADYIE MLSLAKQW+WIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSDSLVVSGPLWTGPLHSADYIENMLSLAKQWKWIGNGQGKDLEKLLGQ 360
Query: 361 MVDESDPKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
M+DESD KLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MMDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
Query: 421 AECIRIALELQHSCLDVHQV 441
AECIRIALELQ +CL HQV
Sbjct: 421 AECIRIALELQQNCLGFHQV 440
BLAST of Spg028496 vs. NCBI nr
Match:
XP_011655038.1 (tRNA (guanine(26)-N(2))-dimethyltransferase [Cucumis sativus] >XP_011655039.1 tRNA (guanine(26)-N(2))-dimethyltransferase [Cucumis sativus] >KGN50729.1 hypothetical protein Csa_005829 [Cucumis sativus])
HSP 1 Score: 790.8 bits (2041), Expect = 6.0e-225
Identity = 389/440 (88.41%), Postives = 412/440 (93.64%), Query Frame = 0
Query: 1 MLTVTPKTLSPSPFLHSPQTNSHCLTSSTIHLFRSSKWMKKSLNNCLSNNRTERGVEFDI 60
MLTV P+TLSPSPFLH PQTNSH LTSS+IHLF+ S K LNNCLSNNRTERG++FDI
Sbjct: 1 MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 60
Query: 61 GDTFFRHESATGRDLGVLAAALYRKSKGSIRVLDALCGCGIRSLRYLVEAEADFLWANDA 120
GDTFFRHESATGRDLGVLAAALYRKSKG +RVLDALCGCGIRSLRYLVE+EADF+ ANDA
Sbjct: 61 GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 120
Query: 121 NDENRRVIVKNLSRVGRGFGDDRRWVVTNCDANRVMTEIYLHKDFFDFIDVDSFGSDSTF 180
NDENRRVIVKNLSRV GD+RRW+VTNCDANRVMTE YL KDFFDFIDVDSFGSDS+F
Sbjct: 121 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
Query: 181 LRSAINALKLGGLLYVTSTDGFSSGGHRPSQSLGAYGAYVRPMPFSNEIGLRMLIGGVVR 240
LR AINALKL GLLYVTSTDGFSSGGHRPSQSL AYGAYVRPMPFSNEIGLRMLIGGV R
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLQINRGKIYKDRHYGFISYCEKCGISQAFSWSEL 300
EASVLG+YATPLFSYYSYHGPVFRVML+INRG+I+K+RHYGF+SYCEKCG SQAFSWSEL
Sbjct: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 300
Query: 301 GQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEEMLSLAKQWEWIGNGQGKDLEKLLRQ 360
GQMSCPCNDSRVSESLVVSGPLW GPLHSADYIE++L+LAKQWEWIGNGQGKDLEKLLRQ
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 360
Query: 361 MVDESDPKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
MVDESD KLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
Query: 421 AECIRIALELQHSCLDVHQV 441
AECIRIA +LQ +CL HQV
Sbjct: 421 AECIRIA-QLQRNCLGAHQV 439
BLAST of Spg028496 vs. NCBI nr
Match:
XP_022943113.1 (tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita moschata])
HSP 1 Score: 788.1 bits (2034), Expect = 3.9e-224
Identity = 387/439 (88.15%), Postives = 407/439 (92.71%), Query Frame = 0
Query: 1 MLTVTPKTLSPSPFLHSPQTNSHCLTSSTIHLFRSSKWMKKSLNNCLSNNRTERGVEFDI 60
MLTV TLSPSPFL + QT SHC +SSTIHLF+ S+ KK LNNCL NNRTERG+EFDI
Sbjct: 1 MLTVMLNTLSPSPFLRTQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60
Query: 61 GDTFFRHESATGRDLGVLAAALYRKSKGSIRVLDALCGCGIRSLRYLVEAEADFLWANDA 120
GDTFFRHESATGRDLGVLAAALYRKSKGS+RVLDALCGCGIRSLRYLVEAEADF+ ANDA
Sbjct: 61 GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120
Query: 121 NDENRRVIVKNLSRVGRGFGDDRRWVVTNCDANRVMTEIYLHKDFFDFIDVDSFGSDSTF 180
NDENR VIVKNLSRV GFG+ RRW+VTNCDANRVM+E YL KDFFDFIDVDSFGSDSTF
Sbjct: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180
Query: 181 LRSAINALKLGGLLYVTSTDGFSSGGHRPSQSLGAYGAYVRPMPFSNEIGLRMLIGGVVR 240
LR AINALKL GLLYVTSTDGFSSGGH PSQSL AYGAYVRPMPFSNEIGLRMLIGGV R
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLQINRGKIYKDRHYGFISYCEKCGISQAFSWSEL 300
EASVLG+Y TPLFSYYSYHGPVFRVML+INRG+I K+RHYGF+SYC+KCG SQAFSWSE+
Sbjct: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRINRGRINKNRHYGFVSYCKKCGNSQAFSWSEV 300
Query: 301 GQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEEMLSLAKQWEWIGNGQGKDLEKLLRQ 360
GQMSCPCND+RVSESLVVSGPLWTGPLHSADYIE+ML+LA QWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360
Query: 361 MVDESDPKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
MVDESD KLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
Query: 421 AECIRIALELQHSCLDVHQ 440
AECIRIALELQ SCL VHQ
Sbjct: 421 AECIRIALELQQSCLGVHQ 439
BLAST of Spg028496 vs. NCBI nr
Match:
XP_022986521.1 (tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita maxima])
HSP 1 Score: 787.3 bits (2032), Expect = 6.7e-224
Identity = 387/440 (87.95%), Postives = 407/440 (92.50%), Query Frame = 0
Query: 1 MLTVTPKTLSPSPFLHSPQTNSHCLTSSTIHLFRSSKWMKKSLNNCLSNNRTERGVEFDI 60
MLTV TLSPSPFL S QT SHC +SSTIHLF+ S+ KK LNNCL NNRTERG+EFDI
Sbjct: 1 MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60
Query: 61 GDTFFRHESATGRDLGVLAAALYRKSKGSIRVLDALCGCGIRSLRYLVEAEADFLWANDA 120
GDTFFRHESATGRDLGVLAAALYRKSKGS+RVLDALCGCGIRSLRYLVEAEADF+ ANDA
Sbjct: 61 GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120
Query: 121 NDENRRVIVKNLSRVGRGFGDDRRWVVTNCDANRVMTEIYLHKDFFDFIDVDSFGSDSTF 180
NDENR VIVKNLSRV GFG+ RRW+VTNCDANRVM+E YL KDFFDFIDVDSFGSDSTF
Sbjct: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180
Query: 181 LRSAINALKLGGLLYVTSTDGFSSGGHRPSQSLGAYGAYVRPMPFSNEIGLRMLIGGVVR 240
LR AINALKL GLLYVTSTDGFSSGGH PSQSL AYGAYVRPMPFSNEIGLRMLIGGV R
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLQINRGKIYKDRHYGFISYCEKCGISQAFSWSEL 300
EASVLG+Y TPLFSYYSYHGPVFRVML+I+RG+I K+RHYGF+SYC+KCG SQ FSWSE+
Sbjct: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSWSEV 300
Query: 301 GQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEEMLSLAKQWEWIGNGQGKDLEKLLRQ 360
GQMSCPCND+RVSESLVVSGPLWTGPLHSADYIE+ML+LA QWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360
Query: 361 MVDESDPKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
MVDESD KLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
Query: 421 AECIRIALELQHSCLDVHQV 441
AECIRIALELQ SCL VHQV
Sbjct: 421 AECIRIALELQQSCLGVHQV 440
BLAST of Spg028496 vs. NCBI nr
Match:
XP_023529370.1 (tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita pepo subsp. pepo])
HSP 1 Score: 785.0 bits (2026), Expect = 3.3e-223
Identity = 386/440 (87.73%), Postives = 407/440 (92.50%), Query Frame = 0
Query: 1 MLTVTPKTLSPSPFLHSPQTNSHCLTSSTIHLFRSSKWMKKSLNNCLSNNRTERGVEFDI 60
MLTV TLSPSPFL S QT SHC +SSTIHLF+ S+ KSLNNCL NNRTERG+EFDI
Sbjct: 1 MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQIKSLNNCLPNNRTERGIEFDI 60
Query: 61 GDTFFRHESATGRDLGVLAAALYRKSKGSIRVLDALCGCGIRSLRYLVEAEADFLWANDA 120
GDTFFRHESATGRDLGVLAAALYRKSKGS+RVLDALCGCGIRSLRYLVEAEADF+ ANDA
Sbjct: 61 GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120
Query: 121 NDENRRVIVKNLSRVGRGFGDDRRWVVTNCDANRVMTEIYLHKDFFDFIDVDSFGSDSTF 180
NDENR VIVKNLS+V GFG+ RRW+VTNCDANRVM+E YL KDFFDFIDVDSFGSDSTF
Sbjct: 121 NDENRGVIVKNLSKVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180
Query: 181 LRSAINALKLGGLLYVTSTDGFSSGGHRPSQSLGAYGAYVRPMPFSNEIGLRMLIGGVVR 240
LR AINALKL GLLYVTSTDGFSSGGH PSQSL AYGAYVRPMPFSNEIGLRMLIGGV R
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLQINRGKIYKDRHYGFISYCEKCGISQAFSWSEL 300
EASVLG+Y TPLFSYYSYHGPVFRVML+I+RG+I K+RHYGF+SYC+KCG SQAFSWSE+
Sbjct: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQAFSWSEV 300
Query: 301 GQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEEMLSLAKQWEWIGNGQGKDLEKLLRQ 360
GQMSCPCND+RVSESLVVSGPLWTGPLHSADYIE+ML+LA QWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360
Query: 361 MVDESDPKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
MVDESD KLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
Query: 421 AECIRIALELQHSCLDVHQV 441
ECIRIALELQ SCL VHQV
Sbjct: 421 TECIRIALELQQSCLGVHQV 440
BLAST of Spg028496 vs. ExPASy Swiss-Prot
Match:
B6YUU9 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus onnurineus (strain NA1) OX=523850 GN=trm1 PE=3 SV=1)
HSP 1 Score: 156.4 bits (394), Expect = 7.6e-37
Identity = 125/379 (32.98%), Postives = 184/379 (48.55%), Query Frame = 0
Query: 64 FFRHESATGRDLGVLAAALYRKSKGSIRVLDALCGCGIRSLRYLVEAEADFLWANDANDE 123
F+ A RD+ VLA K+ GS RVLDAL GIR +RY +E A +W ND N
Sbjct: 27 FYNPLMALNRDISVLAL----KALGSRRVLDALSATGIRGIRYALETPAGEIWLNDINPN 86
Query: 124 NRRVIVKNLSRVGRG----------FGDDRRWVVTNCDANRVMTEIYLHKDFFDFIDVDS 183
R+IV N+ G ++ VVTN DANR+M E + +FDF+D+D
Sbjct: 87 AFRLIVDNVKLNFSGELEIEGKMAVLEGEKTLVVTNADANRLMNEQF---RYFDFVDLDP 146
Query: 184 FGSDSTFLRSAINALKLGGLLYVTSTD-GFSSGGHRPSQSLGAYGAYVRPMPFSNEIGLR 243
FGS FL SA+ A+K G+L +T+TD G G +R + L Y A +E GLR
Sbjct: 147 FGSPMEFLDSALRAVKRRGVLAITATDTGVLCGAYR-NACLRKYLAEPIRGELCHEAGLR 206
Query: 244 MLIGGVVREASVLGYYATPLFSYYSYHGPVFRVMLQINRGKIYKDRHYGFISYCEKCGIS 303
+LIG +VR A+ L +YY H FR LQ+ G D+ + Y +
Sbjct: 207 ILIGTLVRYAAKYDLGVEVLLAYYRDH--YFRAFLQLRNGAKTADKSMKQLGYLYQ---- 266
Query: 304 QAFSWSELGQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEEMLSLAKQWEWIGNGQGK 363
E G+ ++ + GPLW GPL + +++EE+ L+ + +G+ K
Sbjct: 267 -----DESGKFE--FENTFLPSKATAHGPLWLGPLKNQEFVEELHRLSAEHP-LGS---K 326
Query: 364 DLEKLLRQMVDESDPKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASN 423
+K L + DE + +P Y VA R + L ++ + + GY A+R+H +
Sbjct: 327 KTQKFLGLLKDELE--VPFFY-DTHAVARRHGLEVRKLAGVIEILYEKGYKATRTHFSPT 377
Query: 424 AIKTNCPMAECIRIALELQ 432
A+KT+ P E + ELQ
Sbjct: 387 ALKTDAPFEEVLEALRELQ 377
BLAST of Spg028496 vs. ExPASy Swiss-Prot
Match:
P81554 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) OX=186497 GN=trm1 PE=1 SV=1)
HSP 1 Score: 144.8 bits (364), Expect = 2.3e-33
Identity = 114/369 (30.89%), Postives = 173/369 (46.88%), Query Frame = 0
Query: 64 FFRHESATGRDLGVLAAALYRKSKGSIRVLDALCGCGIRSLRYLVEAEADFLWANDANDE 123
F+ A RD+ VL L K I VLDAL GIR +R+ +E A+ +W ND N+
Sbjct: 29 FYNPRMAPNRDVVVL---LLNVLKPKI-VLDALSATGIRGIRFALETPAEEIWMNDINEL 88
Query: 124 NRRVIVKNLSRVGRG----------FGDDRRWVVTNCDANRVMTEIYLHKDFFDFIDVDS 183
++ KN+ +G F ++ V+ N DANR+M E + +F FID+D
Sbjct: 89 AYELMKKNVLLNFKGTLKENAKRAIFEGEKTIVINNDDANRLMAEKH---RYFHFIDLDP 148
Query: 184 FGSDSTFLRSAINALKLGGLLYVTSTDGFSSGGHRPSQSLGAYGAYVRPMPFSNEIGLRM 243
FGS FL +A+ ++K G+L VT+TDG G P L Y A +E+G R+
Sbjct: 149 FGSPMEFLDTALRSVKRKGILGVTATDGAPLCGAHPKACLRKYLAVPLRGELCHEVGTRI 208
Query: 244 LIGGVVREASVLGYYATPLFSYYSYHGPVFRVMLQINRGKIYKD---RHYGFISYCEKCG 303
L+G + R A+ L +YY H FR +++ G D + G++ + EK G
Sbjct: 209 LVGVIARYAAKYDLGMEVLLAYYKDH--YFRAFVKLKDGAKKGDETLENLGYVYFDEKTG 268
Query: 304 ISQAFSWSELGQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEEMLSLAKQWEWIGNGQ 363
+ S +S GPLW GPL + + EML L + N
Sbjct: 269 RFEV-------------EKSFLSTRPNAYGPLWLGPLKNEKVVGEMLELLVSGFEVAN-- 328
Query: 364 GKDLEKLLRQMVDESDPKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIA 420
+++ KLL + +E D +P+ Y + R K+ L ++ E+ GY A+R+H +
Sbjct: 329 YREVLKLLHMLHEELD--IPLFY-DTHALGKRLKIEPKKLGEIIKELKSMGYEATRTHFS 370
BLAST of Spg028496 vs. ExPASy Swiss-Prot
Match:
Q9V1P3 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus abyssi (strain GE5 / Orsay) OX=272844 GN=trm1 PE=3 SV=2)
HSP 1 Score: 142.9 bits (359), Expect = 8.7e-33
Identity = 108/369 (29.27%), Postives = 177/369 (47.97%), Query Frame = 0
Query: 64 FFRHESATGRDLGVLAAALYRKSKGSIRVLDALCGCGIRSLRYLVEAEADFLWANDANDE 123
F+ A RD+ V+ L K I VLDAL GIR +R+ +E A+ +W ND +++
Sbjct: 27 FYNPRMALNRDIAVV---LLNVLKPRI-VLDALSATGIRGIRFALETPAEEIWMNDISED 86
Query: 124 NRRVIVKNLSRVGRG----------FGDDRRWVVTNCDANRVMTEIYLHKDFFDFIDVDS 183
++ KN+ +G ++ VV + DANR+M E + +F FID+D
Sbjct: 87 AYNLMKKNVLLNFKGELEESNGRAVLKSEKTLVVNHDDANRLMAEKH---RYFHFIDLDP 146
Query: 184 FGSDSTFLRSAINALKLGGLLYVTSTDGFSSGGHRPSQSLGAYGAYVRPMPFSNEIGLRM 243
FGS FL +A+ ++K G+L +T+TDG G P + Y A +E+G R+
Sbjct: 147 FGSPMEFLDTALRSVKRKGILGITATDGAPLCGAHPKACMRKYLAVPLRGELCHEVGTRI 206
Query: 244 LIGGVVREASVLGYYATPLFSYYSYHGPVFRVMLQINRGKIYKD---RHYGFISYCEKCG 303
L+G V R A+ + +YY H FR +++ G D + G+I + E G
Sbjct: 207 LVGVVARYAAKYDLGIEVILAYYKDH--YFRAFIKLKDGARKGDESLENLGYIYFDESTG 266
Query: 304 ISQAFSWSELGQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEEMLSLAKQWEWIGNGQ 363
E+ + P + GP+W GPL S + +EEML +++Q + +
Sbjct: 267 ------KFEVERSFLPSKPN-------AYGPVWLGPLKSQEIVEEMLEISQQ---LSLAR 326
Query: 364 GKDLEKLLRQMVDESDPKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIA 420
K KLL+ + DE D L + + R K+ + + +++E+ GY ASR+H +
Sbjct: 327 KKQAVKLLKILKDELDVPL---FYDTHGLGRRLKIEARKIEEIINELRSLGYRASRTHFS 367
BLAST of Spg028496 vs. ExPASy Swiss-Prot
Match:
C5A6N2 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3) OX=593117 GN=trm1 PE=3 SV=1)
HSP 1 Score: 135.6 bits (340), Expect = 1.4e-30
Identity = 115/376 (30.59%), Postives = 178/376 (47.34%), Query Frame = 0
Query: 64 FFRHESATGRDLGVLAAALYRKSKGSIRVLDALCGCGIRSLRYLVEAEADFLWANDANDE 123
F+ A RD+ VLA K+ RVLDAL GIR LRY +E A+ +W ND N++
Sbjct: 27 FYNPVMALNRDISVLAV----KALSPRRVLDALSATGIRGLRYSLETSAEEVWMNDINED 86
Query: 124 NRRVIVKNL------------SRVGRGFGDDRRWVVTNCDANRVMTEIYLHKDFFDFIDV 183
R+I+KNL R G ++R VV DAN++M+E + +FDFID+
Sbjct: 87 AFRLILKNLELNFGSKPVLANGRAELGL-PEKRLVVNLGDANKLMSENF---RYFDFIDL 146
Query: 184 DSFGSDSTFLRSAINALKLGGLLYVTSTD-GFSSGGHRPSQSLGAYGAYVRPMPFSNEIG 243
D FGS FL SA+ +++ G L +T+TD G G +R + +R +E G
Sbjct: 147 DPFGSPVEFLDSALRSVRRRGFLGLTATDTGVLCGAYRRACRRKYLAEPIRG-ELCHEAG 206
Query: 244 LRMLIGGVVREASVLGYYATPLFSYYSYHGPVFRVMLQINRGKIYKDRHYGFISYCEKCG 303
LR+LIG +VR A+ L +YY H FRV L++ G D + Y
Sbjct: 207 LRILIGTIVRYAAKYDLGVEVLLAYYRDH--YFRVFLRLRSGAKKADESVELLGYL---- 266
Query: 304 ISQAFSW-SELGQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEEMLSLAKQWEWIGNG 363
W E G+ + GP+W GPL + ++EE++ L K E +
Sbjct: 267 ------WQDETGKFK--YERDFLPGKRGAYGPMWLGPLKNQAFLEELVRLTKTSE--ESI 326
Query: 364 QGKDLEKLLRQMVDESDPKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHI 423
+ + L +V+E D +P Y +A + + ++ ++ + G+ A+R+H
Sbjct: 327 SHRKTFQFLSLLVEELD--VPFHY-DTHALARKNGLEVVKISRVIETLHSLGFEATRTHF 374
Query: 424 ASNAIKTNCPMAECIR 426
+ +IKTN P +R
Sbjct: 387 SPTSIKTNAPFDVVLR 374
BLAST of Spg028496 vs. ExPASy Swiss-Prot
Match:
Q97ZH0 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) OX=273057 GN=trm1 PE=3 SV=1)
HSP 1 Score: 134.8 bits (338), Expect = 2.4e-30
Identity = 101/360 (28.06%), Postives = 181/360 (50.28%), Query Frame = 0
Query: 64 FFRHESATGRDLGVLAAALYRKSKGSIRVLDALCGCGIRSLRYLVEA-EADFLWANDAND 123
F+ + RDL V+ ++ + ++DAL GIR +RY VE+ +++ L ND N
Sbjct: 35 FYNPKMTFNRDLSVIVVSILKPK----IIVDALSATGIRGIRYYVESWKSEQLILNDKNP 94
Query: 124 ENRRVIVKNLSRVGRGFGDDRRWVVTNCDANRVMTEIYLHKDFFDFIDVDSFGSDSTFLR 183
+I N G + N DAN ++ E+ D+ID+D FGS + F+
Sbjct: 95 NATSLIQINAKNNG-----IENAKIYNKDANALLYEVK-----SDYIDIDPFGSPAPFIL 154
Query: 184 SAINALKLGGLLYVTSTDGFSSGGHRPSQSLGAYGAYVRPMPFSNEIGLRMLIGGVVREA 243
S++NA G++ T+TD G P+ Y A + S E+GLR+LIG ++REA
Sbjct: 155 SSLNAATRNGIVAFTATDLSPLEGSSPTSCRRKYDAINHKLSSSKELGLRVLIGKIIREA 214
Query: 244 SVLGYYATPLFSYYSYHGPVFRVMLQINRGKIYKD----RHYGFISYCEKCGISQAFSWS 303
++L PLFS+Y+ + +R+ +++ G D +H + C +CG Q F
Sbjct: 215 AILEKTVYPLFSFYADY--YYRLFVRVENGARKADDNINKHLKYFGECPRCGF-QTFV-E 274
Query: 304 ELGQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEEMLSLAKQWEWIGNGQGKDLEKLL 363
E + CP E + GPL+ GPL+S ++++ ++ L + ++ + ++KLL
Sbjct: 275 ENCKTKCPV----CGEIFSIIGPLYIGPLYSMEFLKRIMDLYSNFNYLTS--FNRIQKLL 334
Query: 364 RQMVDESDPKLPVGYIKMDEVASRAKVNS-PPLNTMMSEMIKAGYAASRSHIASNAIKTN 418
V E + + + + ++AS+ K+++ PP+ +++ + ASR+H A I+T+
Sbjct: 335 N--VIEKEARFKNVFYNISKLASKLKISAIPPIESILECL----GDASRTHFAPTGIRTD 364
BLAST of Spg028496 vs. ExPASy TrEMBL
Match:
A0A0A0KP99 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G220920 PE=3 SV=1)
HSP 1 Score: 790.8 bits (2041), Expect = 2.9e-225
Identity = 389/440 (88.41%), Postives = 412/440 (93.64%), Query Frame = 0
Query: 1 MLTVTPKTLSPSPFLHSPQTNSHCLTSSTIHLFRSSKWMKKSLNNCLSNNRTERGVEFDI 60
MLTV P+TLSPSPFLH PQTNSH LTSS+IHLF+ S K LNNCLSNNRTERG++FDI
Sbjct: 1 MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 60
Query: 61 GDTFFRHESATGRDLGVLAAALYRKSKGSIRVLDALCGCGIRSLRYLVEAEADFLWANDA 120
GDTFFRHESATGRDLGVLAAALYRKSKG +RVLDALCGCGIRSLRYLVE+EADF+ ANDA
Sbjct: 61 GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 120
Query: 121 NDENRRVIVKNLSRVGRGFGDDRRWVVTNCDANRVMTEIYLHKDFFDFIDVDSFGSDSTF 180
NDENRRVIVKNLSRV GD+RRW+VTNCDANRVMTE YL KDFFDFIDVDSFGSDS+F
Sbjct: 121 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
Query: 181 LRSAINALKLGGLLYVTSTDGFSSGGHRPSQSLGAYGAYVRPMPFSNEIGLRMLIGGVVR 240
LR AINALKL GLLYVTSTDGFSSGGHRPSQSL AYGAYVRPMPFSNEIGLRMLIGGV R
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLQINRGKIYKDRHYGFISYCEKCGISQAFSWSEL 300
EASVLG+YATPLFSYYSYHGPVFRVML+INRG+I+K+RHYGF+SYCEKCG SQAFSWSEL
Sbjct: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 300
Query: 301 GQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEEMLSLAKQWEWIGNGQGKDLEKLLRQ 360
GQMSCPCNDSRVSESLVVSGPLW GPLHSADYIE++L+LAKQWEWIGNGQGKDLEKLLRQ
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 360
Query: 361 MVDESDPKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
MVDESD KLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
Query: 421 AECIRIALELQHSCLDVHQV 441
AECIRIA +LQ +CL HQV
Sbjct: 421 AECIRIA-QLQRNCLGAHQV 439
BLAST of Spg028496 vs. ExPASy TrEMBL
Match:
A0A6J1FWD4 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111447941 PE=3 SV=1)
HSP 1 Score: 788.1 bits (2034), Expect = 1.9e-224
Identity = 387/439 (88.15%), Postives = 407/439 (92.71%), Query Frame = 0
Query: 1 MLTVTPKTLSPSPFLHSPQTNSHCLTSSTIHLFRSSKWMKKSLNNCLSNNRTERGVEFDI 60
MLTV TLSPSPFL + QT SHC +SSTIHLF+ S+ KK LNNCL NNRTERG+EFDI
Sbjct: 1 MLTVMLNTLSPSPFLRTQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60
Query: 61 GDTFFRHESATGRDLGVLAAALYRKSKGSIRVLDALCGCGIRSLRYLVEAEADFLWANDA 120
GDTFFRHESATGRDLGVLAAALYRKSKGS+RVLDALCGCGIRSLRYLVEAEADF+ ANDA
Sbjct: 61 GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120
Query: 121 NDENRRVIVKNLSRVGRGFGDDRRWVVTNCDANRVMTEIYLHKDFFDFIDVDSFGSDSTF 180
NDENR VIVKNLSRV GFG+ RRW+VTNCDANRVM+E YL KDFFDFIDVDSFGSDSTF
Sbjct: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180
Query: 181 LRSAINALKLGGLLYVTSTDGFSSGGHRPSQSLGAYGAYVRPMPFSNEIGLRMLIGGVVR 240
LR AINALKL GLLYVTSTDGFSSGGH PSQSL AYGAYVRPMPFSNEIGLRMLIGGV R
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLQINRGKIYKDRHYGFISYCEKCGISQAFSWSEL 300
EASVLG+Y TPLFSYYSYHGPVFRVML+INRG+I K+RHYGF+SYC+KCG SQAFSWSE+
Sbjct: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRINRGRINKNRHYGFVSYCKKCGNSQAFSWSEV 300
Query: 301 GQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEEMLSLAKQWEWIGNGQGKDLEKLLRQ 360
GQMSCPCND+RVSESLVVSGPLWTGPLHSADYIE+ML+LA QWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360
Query: 361 MVDESDPKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
MVDESD KLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
Query: 421 AECIRIALELQHSCLDVHQ 440
AECIRIALELQ SCL VHQ
Sbjct: 421 AECIRIALELQQSCLGVHQ 439
BLAST of Spg028496 vs. ExPASy TrEMBL
Match:
A0A6J1JBD3 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111484232 PE=3 SV=1)
HSP 1 Score: 787.3 bits (2032), Expect = 3.2e-224
Identity = 387/440 (87.95%), Postives = 407/440 (92.50%), Query Frame = 0
Query: 1 MLTVTPKTLSPSPFLHSPQTNSHCLTSSTIHLFRSSKWMKKSLNNCLSNNRTERGVEFDI 60
MLTV TLSPSPFL S QT SHC +SSTIHLF+ S+ KK LNNCL NNRTERG+EFDI
Sbjct: 1 MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60
Query: 61 GDTFFRHESATGRDLGVLAAALYRKSKGSIRVLDALCGCGIRSLRYLVEAEADFLWANDA 120
GDTFFRHESATGRDLGVLAAALYRKSKGS+RVLDALCGCGIRSLRYLVEAEADF+ ANDA
Sbjct: 61 GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120
Query: 121 NDENRRVIVKNLSRVGRGFGDDRRWVVTNCDANRVMTEIYLHKDFFDFIDVDSFGSDSTF 180
NDENR VIVKNLSRV GFG+ RRW+VTNCDANRVM+E YL KDFFDFIDVDSFGSDSTF
Sbjct: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180
Query: 181 LRSAINALKLGGLLYVTSTDGFSSGGHRPSQSLGAYGAYVRPMPFSNEIGLRMLIGGVVR 240
LR AINALKL GLLYVTSTDGFSSGGH PSQSL AYGAYVRPMPFSNEIGLRMLIGGV R
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLQINRGKIYKDRHYGFISYCEKCGISQAFSWSEL 300
EASVLG+Y TPLFSYYSYHGPVFRVML+I+RG+I K+RHYGF+SYC+KCG SQ FSWSE+
Sbjct: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSWSEV 300
Query: 301 GQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEEMLSLAKQWEWIGNGQGKDLEKLLRQ 360
GQMSCPCND+RVSESLVVSGPLWTGPLHSADYIE+ML+LA QWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360
Query: 361 MVDESDPKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
MVDESD KLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
Query: 421 AECIRIALELQHSCLDVHQV 441
AECIRIALELQ SCL VHQV
Sbjct: 421 AECIRIALELQQSCLGVHQV 440
BLAST of Spg028496 vs. ExPASy TrEMBL
Match:
A0A1S3C509 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103496736 PE=3 SV=1)
HSP 1 Score: 778.9 bits (2010), Expect = 1.2e-221
Identity = 383/440 (87.05%), Postives = 409/440 (92.95%), Query Frame = 0
Query: 1 MLTVTPKTLSPSPFLHSPQTNSHCLTSSTIHLFRSSKWMKKSLNNCLSNNRTERGVEFDI 60
MLTV TLSPSPFL+ QTNSH LTS +IHLF+ S KKS NNCLSNNRTERG++FDI
Sbjct: 1 MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60
Query: 61 GDTFFRHESATGRDLGVLAAALYRKSKGSIRVLDALCGCGIRSLRYLVEAEADFLWANDA 120
GDTFFRHESATGRDLGVLAAALYRKSKG +R+LDALCGCGIRSLRYLVE+EADF+ ANDA
Sbjct: 61 GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120
Query: 121 NDENRRVIVKNLSRVGRGFGDDRRWVVTNCDANRVMTEIYLHKDFFDFIDVDSFGSDSTF 180
NDENRRVIVKNLS+V FGD+RRW+VTNCDANRVMTE YL KDFFDFIDVDSFGSDS+F
Sbjct: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
Query: 181 LRSAINALKLGGLLYVTSTDGFSSGGHRPSQSLGAYGAYVRPMPFSNEIGLRMLIGGVVR 240
LR AINALKL GLLYVTSTDGFSSGGHRPSQSL AYGAY+RPMPFSNEIGLRMLIGGV R
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240
Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLQINRGKIYKDRHYGFISYCEKCGISQAFSWSEL 300
EASVLGYYATPLFSYYSYHGPVFRVML+INRG+I K+RHYGF+SYCEKCG SQAFSWSEL
Sbjct: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300
Query: 301 GQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEEMLSLAKQWEWIGNGQGKDLEKLLRQ 360
GQMSCPCNDSRVSESLVVSGPLW GPLHSADYIE++L+LAKQWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360
Query: 361 MVDESDPKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
M+DESD KLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
Query: 421 AECIRIALELQHSCLDVHQV 441
A+CIRIA +LQ +CL VHQV
Sbjct: 421 ADCIRIA-QLQQNCLGVHQV 439
BLAST of Spg028496 vs. ExPASy TrEMBL
Match:
A0A5D3DFE8 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold530G00120 PE=3 SV=1)
HSP 1 Score: 778.9 bits (2010), Expect = 1.2e-221
Identity = 383/440 (87.05%), Postives = 409/440 (92.95%), Query Frame = 0
Query: 1 MLTVTPKTLSPSPFLHSPQTNSHCLTSSTIHLFRSSKWMKKSLNNCLSNNRTERGVEFDI 60
MLTV TLSPSPFL+ QTNSH LTS +IHLF+ S KKS NNCLSNNRTERG++FDI
Sbjct: 1 MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60
Query: 61 GDTFFRHESATGRDLGVLAAALYRKSKGSIRVLDALCGCGIRSLRYLVEAEADFLWANDA 120
GDTFFRHESATGRDLGVLAAALYRKSKG +R+LDALCGCGIRSLRYLVE+EADF+ ANDA
Sbjct: 61 GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120
Query: 121 NDENRRVIVKNLSRVGRGFGDDRRWVVTNCDANRVMTEIYLHKDFFDFIDVDSFGSDSTF 180
NDENRRVIVKNLS+V FGD+RRW+VTNCDANRVMTE YL KDFFDFIDVDSFGSDS+F
Sbjct: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
Query: 181 LRSAINALKLGGLLYVTSTDGFSSGGHRPSQSLGAYGAYVRPMPFSNEIGLRMLIGGVVR 240
LR AINALKL GLLYVTSTDGFSSGGHRPSQSL AYGAY+RPMPFSNEIGLRMLIGGV R
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240
Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLQINRGKIYKDRHYGFISYCEKCGISQAFSWSEL 300
EASVLGYYATPLFSYYSYHGPVFRVML+INRG+I K+RHYGF+SYCEKCG SQAFSWSEL
Sbjct: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300
Query: 301 GQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEEMLSLAKQWEWIGNGQGKDLEKLLRQ 360
GQMSCPCNDSRVSESLVVSGPLW GPLHSADYIE++L+LAKQWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360
Query: 361 MVDESDPKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
M+DESD KLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
Query: 421 AECIRIALELQHSCLDVHQV 441
A+CIRIA +LQ +CL VHQV
Sbjct: 421 ADCIRIA-QLQQNCLGVHQV 439
BLAST of Spg028496 vs. TAIR 10
Match:
AT3G56330.1 (N2,N2-dimethylguanosine tRNA methyltransferase )
HSP 1 Score: 577.4 bits (1487), Expect = 9.8e-165
Identity = 281/437 (64.30%), Postives = 345/437 (78.95%), Query Frame = 0
Query: 1 MLTVTPKTLSPSPF--LHSPQTNSHCLTSSTIHLFRSSKWMKKSLNNCL--SNNRTERGV 60
+LT++PKTLS S F +H Q N C + + K +K S + L S + ER +
Sbjct: 2 LLTLSPKTLSSSSFFTVHKSQ-NPKCKSPDSCRF----KSLKCSFDRILVKSEVQIERNL 61
Query: 61 EFDIGDTFFRHESATGRDLGVLAAALYRKSKGSIRVLDALCGCGIRSLRYLVEAEADFLW 120
EF+ G+TFFRHESA GRDLGVL+A LY++S GS+RVLDA+CGCGIRSLRYLVEAEADF+
Sbjct: 62 EFETGETFFRHESARGRDLGVLSATLYKRSNGSLRVLDAMCGCGIRSLRYLVEAEADFVM 121
Query: 121 ANDANDENRRVIVKNLSRVGRGFGDDRRWVVTNCDANRVMTEIYLHKDFFDFIDVDSFGS 180
ANDAND+NRRVI NLS+V RG GD+RRWVVT+ AN+ M E Y+ DFFD ID+DSFGS
Sbjct: 122 ANDANDDNRRVITDNLSKVERGTGDERRWVVTHMLANKAMIERYMVADFFDMIDIDSFGS 181
Query: 181 DSTFLRSAINALKLGGLLYVTSTDGFSSGGHRPSQSLGAYGAYVRPMPFSNEIGLRMLIG 240
DS+FLR A NAL+LGGLLY+TSTDG+SSGGHRP SL AYGA++RPMPF NEIGLRMLIG
Sbjct: 182 DSSFLRDAFNALRLGGLLYLTSTDGYSSGGHRPYNSLAAYGAFIRPMPFGNEIGLRMLIG 241
Query: 241 GVVREASVLGYYATPLFSYYSYHGPVFRVMLQINRGKIYKDRHYGFISYCEKCGISQAFS 300
G VREA++LGY+ TPLFSYYSYHGPVFRVML+++RGK+++DR+YGF+++C CG S
Sbjct: 242 GAVREAALLGYHVTPLFSYYSYHGPVFRVMLRVHRGKLHEDRNYGFVTHCNLCGHSHTLR 301
Query: 301 WSELGQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEEMLSLAKQWEWIGNGQGKDLEK 360
+ ELG M CPC+D++ S SLVVSGP+W GPLH A Y+ EML LAK+W W+ G G DL+K
Sbjct: 302 FDELGLMGCPCSDTKASSSLVVSGPMWLGPLHDASYVTEMLELAKEWGWVSEGTGMDLDK 361
Query: 361 LLRQMVDESDPKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKT 420
LL M++ESDP+LP GY KMDE+ASRAK+NSPPL MMS ++K GYAASRSHI NA+KT
Sbjct: 362 LLSIMIEESDPRLPPGYTKMDEMASRAKMNSPPLKKMMSALVKEGYAASRSHIIPNALKT 421
Query: 421 NCPMAECIRIALELQHS 434
+CPM+ +RIA E HS
Sbjct: 422 DCPMSHFVRIAKENLHS 433
BLAST of Spg028496 vs. TAIR 10
Match:
AT5G15810.1 (N2,N2-dimethylguanosine tRNA methyltransferase )
HSP 1 Score: 97.4 bits (241), Expect = 3.0e-20
Identity = 94/387 (24.29%), Postives = 161/387 (41.60%), Query Frame = 0
Query: 59 DIGDTFFRHESATGRDLGVLAAALYRKSKGSI---RVLDALCGCGIRSLRYLVEAEA-DF 118
D G T HE T A Y ++ + RVL+AL G+R+LRY E E
Sbjct: 186 DNGKTNGEHEVTTQDGPKEAAKTAYESARRELKPPRVLEALSASGLRALRYAREVEGIGQ 245
Query: 119 LWANDANDENRRVIVKNLSRVGRGFGDDRRWVVTNCDANRVMTEIYLH-----KDFFDFI 178
+ A D + + +N+ G +++ +T+ +H KD FD +
Sbjct: 246 VVALDNDPASVEACQRNIKFNG---------LMSTSKVESHLTDARVHMLSHPKD-FDVV 305
Query: 179 DVDSFGSDSTFLRSAINALKLGGLLYVTSTDGFSSGGHRPSQSLGAYGAYVRPMPFSNEI 238
D+D +G+ S FL SA+ ++ GGLL T+TD G YG+Y + +E+
Sbjct: 306 DLDPYGAPSIFLDSAVQSVADGGLLMCTATDMAVLCGANGEVCYSKYGSYPLKGKYCHEM 365
Query: 239 GLRMLIGGVVREASVLGYYATPLFSYYSYHGPVFRVMLQINRGKIYKDRHYGFISYCEKC 298
LR+L+ + A+ Y P+ S RV +++ +SY +C
Sbjct: 366 ALRILLASIESHANRYKRYIVPVLSVQMDF--YVRVFVRVYTSASAMKNTPLKLSYVYQC 425
Query: 299 GISQAFSWSELGQMSCPCNDS----------------RVSESLVVSGPLWTGPLHSADYI 358
+F +G+ S P N+S + + GP+W+ P+H +++
Sbjct: 426 IGCDSFHLQSVGR-SLPKNNSVRYLPGVGPVVPQDCTHCGKKYNMGGPIWSAPIHDQEWV 485
Query: 359 EEMLSLAKQWEWIGNGQGKDLEKLLRQMVDESDPKLPVGYIKMDEVASRAKVNSPPLNTM 418
+L+ K + D + + E P +P+ ++ + +++ K SP
Sbjct: 486 NSILNGVKSMK--DRYPAYDRICAVLTTISEELPDVPL-FLSLHSLSATLKCTSPSAALF 545
Query: 419 MSEMIKAGYAASRSHIASNAIKTNCPM 421
S +I A Y S SH+ IKT+ PM
Sbjct: 546 RSAVINAKYRVSGSHVNPLGIKTDAPM 556
BLAST of Spg028496 vs. TAIR 10
Match:
AT3G02320.1 (N2,N2-dimethylguanosine tRNA methyltransferase )
HSP 1 Score: 90.1 bits (222), Expect = 4.7e-18
Identity = 81/348 (23.28%), Postives = 145/348 (41.67%), Query Frame = 0
Query: 91 RVLDALCGCGIRSLRYLVEAEADFLWANDANDENRRVIVKNLSRVGRGFGDDRRWVVTNC 150
+VL+AL G+R+LRY E E ND + ++ R + ++
Sbjct: 123 KVLEALSASGLRALRYAREIEGIGQVVALDND------LASVEACQRNIKFNGSVAISKV 182
Query: 151 DANRVMTEIYL--HKDFFDFIDVDSFGSDSTFLRSAINALKLGGLLYVTSTDGFSSGGHR 210
+++ +++ H FD +D+D +GS S FL SAI ++ GGLL T+TD G
Sbjct: 183 ESHHTDARVHMLTHPKEFDVVDLDPYGSPSIFLDSAIQSVTDGGLLMCTATDMAVLCGGN 242
Query: 211 PSQSLGAYGAYVRPMPFSNEIGLRMLIGGVVREASVLGYYATPLFSYYSYHGPVFRVMLQ 270
YG+Y + +E+ LR+L+ + A+ Y P+ S RV ++
Sbjct: 243 GEVCYSKYGSYPLRAKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDF--YVRVFVR 302
Query: 271 INRGKIYKDRHYGFISYCEKCGISQAFSWSELGQMSCPCNDS----------------RV 330
+ +SY +C +F +G+ S P N+S
Sbjct: 303 VYTSASAMKNTPLKLSYVYQCIGCDSFHLQPVGR-SLPKNNSVRYLPAIGPVVKQDCNHC 362
Query: 331 SESLVVSGPLWTGPLHSADYIEEMLSLAKQWEWIGNGQGKDLEKLLRQMVDESDPKLPVG 390
+ + GP+W+ P+H +++ +L+ K + D + V E +P+
Sbjct: 363 GKKYNMGGPIWSAPMHDPEWVTSILNSVKSMK--DRYPAYDRISAVLTTVSEELLDVPL- 422
Query: 391 YIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 421
++ + + + K SP S +I A Y S +H+ +KT+ PM
Sbjct: 423 FLSLHNLCATLKCISPSAAMFRSAVINANYRISGTHVNPLGMKTDAPM 458
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_038892632.1 | 3.5e-225 | 88.41 | tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Benincasa hispida] | [more] |
| XP_011655038.1 | 6.0e-225 | 88.41 | tRNA (guanine(26)-N(2))-dimethyltransferase [Cucumis sativus] >XP_011655039.1 tR... | [more] |
| XP_022943113.1 | 3.9e-224 | 88.15 | tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita moschata] | [more] |
| XP_022986521.1 | 6.7e-224 | 87.95 | tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita maxima] | [more] |
| XP_023529370.1 | 3.3e-223 | 87.73 | tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita pepo subsp. pepo] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| B6YUU9 | 7.6e-37 | 32.98 | tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus onnurineus (strain N... | [more] |
| P81554 | 2.3e-33 | 30.89 | tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus furiosus (strain ATCC ... | [more] |
| Q9V1P3 | 8.7e-33 | 29.27 | tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus abyssi (strain GE5 / O... | [more] |
| C5A6N2 | 1.4e-30 | 30.59 | tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus gammatolerans (strai... | [more] |
| Q97ZH0 | 2.4e-30 | 28.06 | tRNA (guanine(26)-N(2))-dimethyltransferase OS=Saccharolobus solfataricus (strai... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A0A0KP99 | 2.9e-225 | 88.41 | tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G... | [more] |
| A0A6J1FWD4 | 1.9e-224 | 88.15 | tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC... | [more] |
| A0A6J1JBD3 | 3.2e-224 | 87.95 | tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC11... | [more] |
| A0A1S3C509 | 1.2e-221 | 87.05 | tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103496... | [more] |
| A0A5D3DFE8 | 1.2e-221 | 87.05 | tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo var. makuwa OX=11946... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT3G56330.1 | 9.8e-165 | 64.30 | N2,N2-dimethylguanosine tRNA methyltransferase | [more] |
| AT5G15810.1 | 3.0e-20 | 24.29 | N2,N2-dimethylguanosine tRNA methyltransferase | [more] |
| AT3G02320.1 | 4.7e-18 | 23.28 | N2,N2-dimethylguanosine tRNA methyltransferase | [more] |
