Spg028476 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg028476 vs. NCBI nr
Match: XP_022943023.1 (CRAL-TRIO domain-containing protein YKL091C isoform X1 [Cucurbita moschata] >XP_022943024.1 CRAL-TRIO domain-containing protein YKL091C isoform X1 [Cucurbita moschata] >XP_022943025.1 CRAL-TRIO domain-containing protein YKL091C isoform X1 [Cucurbita moschata] >KAG6600852.1 hypothetical protein SDJN03_06085, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031486.1 hypothetical protein SDJN02_05526 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 445.3 bits (1144), Expect = 3.7e-121
Identity = 222/241 (92.12%), Postives = 233/241 (96.68%), Query Frame = 0

Query: 22  MENTQQISQMRRLVEKNGVSTEKYGDPTMMRFLIARSMDVEKAAKMFVQWQKWRDAMVPN 81
           MEN+Q+I QMRR+VEKNGVSTEKYGD TMMRFLIARSMDVEKAAKMFVQWQKWRD MVPN
Sbjct: 1   MENSQRILQMRRVVEKNGVSTEKYGDLTMMRFLIARSMDVEKAAKMFVQWQKWRDTMVPN 60

Query: 82  GRIDESEIEDELGARKIYLQGLSKNGHSVMIVKGNKHFPAKDQVQFKKYVAFSLDKTISS 141
           GRIDESEIEDEL ARKIYLQGLS+NGHSVMIVKG+KHFPAKDQVQFKKYV ++LDKTISS
Sbjct: 61  GRIDESEIEDELEARKIYLQGLSRNGHSVMIVKGSKHFPAKDQVQFKKYVVYALDKTISS 120

Query: 142 AFQGREIGNEKLIGILDLQQISYKNIDPRGLITGFQFLQAYYPERLAKCYILNMPRFFVS 201
           AFQGREIGNEKLIGILDLQQISYKNIDPRGLITGFQFLQAYYPERLAKCYILNMPRFF+S
Sbjct: 121 AFQGREIGNEKLIGILDLQQISYKNIDPRGLITGFQFLQAYYPERLAKCYILNMPRFFIS 180

Query: 202 VWRMVSRFLEKVTLEKIVIVTNEEERRSMIEEIGEEVLPVEYGGKAKLIALQDAVLPQLE 261
           VWRMVSRFLEKVTLEKIVIV+NEEER+SMIEEIGEEVLP EYGGK KLIALQDAVLP L+
Sbjct: 181 VWRMVSRFLEKVTLEKIVIVSNEEERKSMIEEIGEEVLPAEYGGKGKLIALQDAVLPHLD 240

Query: 262 G 263
           G
Sbjct: 241 G 241

BLAST of Spg028476 vs. NCBI nr
Match: XP_022989029.1 (CRAL-TRIO domain-containing protein YKL091C isoform X1 [Cucurbita maxima] >XP_022989034.1 CRAL-TRIO domain-containing protein YKL091C isoform X1 [Cucurbita maxima] >XP_022989036.1 CRAL-TRIO domain-containing protein YKL091C isoform X1 [Cucurbita maxima])

HSP 1 Score: 445.3 bits (1144), Expect = 3.7e-121
Identity = 222/241 (92.12%), Postives = 233/241 (96.68%), Query Frame = 0

Query: 22  MENTQQISQMRRLVEKNGVSTEKYGDPTMMRFLIARSMDVEKAAKMFVQWQKWRDAMVPN 81
           MENTQ+I QMRR+VEKNGVSTEKYGD TMMRFLIARSMDVEKAAKMFVQWQKWRD MVPN
Sbjct: 1   MENTQRILQMRRVVEKNGVSTEKYGDLTMMRFLIARSMDVEKAAKMFVQWQKWRDTMVPN 60

Query: 82  GRIDESEIEDELGARKIYLQGLSKNGHSVMIVKGNKHFPAKDQVQFKKYVAFSLDKTISS 141
           GRIDESEIEDEL ARKIYLQGLS+NGHSVMIVKG+KHFPAKDQVQFKKYV ++LDKTISS
Sbjct: 61  GRIDESEIEDELEARKIYLQGLSQNGHSVMIVKGSKHFPAKDQVQFKKYVVYALDKTISS 120

Query: 142 AFQGREIGNEKLIGILDLQQISYKNIDPRGLITGFQFLQAYYPERLAKCYILNMPRFFVS 201
           AFQGREIGNEKLIGILDLQQISYKNIDPRGLITGFQFLQAYYPERLAKCYILNMPRFF+S
Sbjct: 121 AFQGREIGNEKLIGILDLQQISYKNIDPRGLITGFQFLQAYYPERLAKCYILNMPRFFIS 180

Query: 202 VWRMVSRFLEKVTLEKIVIVTNEEERRSMIEEIGEEVLPVEYGGKAKLIALQDAVLPQLE 261
           VW+MVSRFLEKVTLEKIVIV+NEEER+SMIEEIGEEVLP EYGGK KLIALQDAVLP L+
Sbjct: 181 VWKMVSRFLEKVTLEKIVIVSNEEERKSMIEEIGEEVLPAEYGGKGKLIALQDAVLPHLD 240

Query: 262 G 263
           G
Sbjct: 241 G 241

BLAST of Spg028476 vs. NCBI nr
Match: XP_023513848.1 (SEC14 cytosolic factor isoform X1 [Cucurbita pepo subsp. pepo] >XP_023513858.1 SEC14 cytosolic factor isoform X1 [Cucurbita pepo subsp. pepo] >XP_023513865.1 SEC14 cytosolic factor isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 444.9 bits (1143), Expect = 4.8e-121
Identity = 222/241 (92.12%), Postives = 233/241 (96.68%), Query Frame = 0

Query: 22  MENTQQISQMRRLVEKNGVSTEKYGDPTMMRFLIARSMDVEKAAKMFVQWQKWRDAMVPN 81
           MEN+Q+I QMRR+VEKNGVSTEKYGD TMMRFLIARSMDVEKAAKMFVQWQKWRD MVPN
Sbjct: 1   MENSQRILQMRRVVEKNGVSTEKYGDLTMMRFLIARSMDVEKAAKMFVQWQKWRDTMVPN 60

Query: 82  GRIDESEIEDELGARKIYLQGLSKNGHSVMIVKGNKHFPAKDQVQFKKYVAFSLDKTISS 141
           GRIDESEIEDEL ARKIYLQGLS+NGHSVMIVKG+KHFPAKDQVQFKKYV ++LDKTISS
Sbjct: 61  GRIDESEIEDELEARKIYLQGLSQNGHSVMIVKGSKHFPAKDQVQFKKYVVYALDKTISS 120

Query: 142 AFQGREIGNEKLIGILDLQQISYKNIDPRGLITGFQFLQAYYPERLAKCYILNMPRFFVS 201
           AFQGREIGNEKLIGILDLQQISYKNIDPRGLITGFQFLQAYYPERLAKCYILNMPRFF+S
Sbjct: 121 AFQGREIGNEKLIGILDLQQISYKNIDPRGLITGFQFLQAYYPERLAKCYILNMPRFFIS 180

Query: 202 VWRMVSRFLEKVTLEKIVIVTNEEERRSMIEEIGEEVLPVEYGGKAKLIALQDAVLPQLE 261
           VWRMVSRFLEKVTLEKIVIV+NEEER+SMIEEIGEEVLP EYGGK KLIALQDAVLP L+
Sbjct: 181 VWRMVSRFLEKVTLEKIVIVSNEEERKSMIEEIGEEVLPAEYGGKGKLIALQDAVLPHLD 240

Query: 262 G 263
           G
Sbjct: 241 G 241

BLAST of Spg028476 vs. NCBI nr
Match: XP_038893279.1 (CRAL-TRIO domain-containing protein YKL091C-like isoform X1 [Benincasa hispida])

HSP 1 Score: 443.7 bits (1140), Expect = 1.1e-120
Identity = 217/241 (90.04%), Postives = 231/241 (95.85%), Query Frame = 0

Query: 22  MENTQQISQMRRLVEKNGVSTEKYGDPTMMRFLIARSMDVEKAAKMFVQWQKWRDAMVPN 81
           MENTQ+I QMRRLVEKNGVS EKYGDPTMMRFL+ARSMDVEKAAKMFVQWQKWRD MVPN
Sbjct: 1   MENTQRIEQMRRLVEKNGVSAEKYGDPTMMRFLVARSMDVEKAAKMFVQWQKWRDTMVPN 60

Query: 82  GRIDESEIEDELGARKIYLQGLSKNGHSVMIVKGNKHFPAKDQVQFKKYVAFSLDKTISS 141
           GRI ESE+EDELG RK+YLQGLSKNGH+V+ +KGNKHFPAKDQVQFKKYV +SLDKTISS
Sbjct: 61  GRIVESEVEDELGTRKMYLQGLSKNGHAVLFLKGNKHFPAKDQVQFKKYVVYSLDKTISS 120

Query: 142 AFQGREIGNEKLIGILDLQQISYKNIDPRGLITGFQFLQAYYPERLAKCYILNMPRFFVS 201
           AF+GREIGNEKLIGILDLQQISYKNIDPRGLITGFQFLQAYYPERLAKCYILNMPRFFVS
Sbjct: 121 AFKGREIGNEKLIGILDLQQISYKNIDPRGLITGFQFLQAYYPERLAKCYILNMPRFFVS 180

Query: 202 VWRMVSRFLEKVTLEKIVIVTNEEERRSMIEEIGEEVLPVEYGGKAKLIALQDAVLPQLE 261
           VWRMVSRFLEK TLEK+VIV+NEEERRSMIEEIGE+VLPVEYGGKAKLIA+QD VLP L+
Sbjct: 181 VWRMVSRFLEKATLEKVVIVSNEEERRSMIEEIGEDVLPVEYGGKAKLIAIQDVVLPHLD 240

Query: 262 G 263
           G
Sbjct: 241 G 241

BLAST of Spg028476 vs. NCBI nr
Match: XP_038893280.1 (CRAL-TRIO domain-containing protein YKL091C-like isoform X2 [Benincasa hispida])

HSP 1 Score: 437.6 bits (1124), Expect = 7.7e-119
Identity = 216/241 (89.63%), Postives = 230/241 (95.44%), Query Frame = 0

Query: 22  MENTQQISQMRRLVEKNGVSTEKYGDPTMMRFLIARSMDVEKAAKMFVQWQKWRDAMVPN 81
           MENTQ+I QMRRLVEKNGVS E YGDPTMMRFL+ARSMDVEKAAKMFVQWQKWRD MVPN
Sbjct: 1   MENTQRIEQMRRLVEKNGVSAE-YGDPTMMRFLVARSMDVEKAAKMFVQWQKWRDTMVPN 60

Query: 82  GRIDESEIEDELGARKIYLQGLSKNGHSVMIVKGNKHFPAKDQVQFKKYVAFSLDKTISS 141
           GRI ESE+EDELG RK+YLQGLSKNGH+V+ +KGNKHFPAKDQVQFKKYV +SLDKTISS
Sbjct: 61  GRIVESEVEDELGTRKMYLQGLSKNGHAVLFLKGNKHFPAKDQVQFKKYVVYSLDKTISS 120

Query: 142 AFQGREIGNEKLIGILDLQQISYKNIDPRGLITGFQFLQAYYPERLAKCYILNMPRFFVS 201
           AF+GREIGNEKLIGILDLQQISYKNIDPRGLITGFQFLQAYYPERLAKCYILNMPRFFVS
Sbjct: 121 AFKGREIGNEKLIGILDLQQISYKNIDPRGLITGFQFLQAYYPERLAKCYILNMPRFFVS 180

Query: 202 VWRMVSRFLEKVTLEKIVIVTNEEERRSMIEEIGEEVLPVEYGGKAKLIALQDAVLPQLE 261
           VWRMVSRFLEK TLEK+VIV+NEEERRSMIEEIGE+VLPVEYGGKAKLIA+QD VLP L+
Sbjct: 181 VWRMVSRFLEKATLEKVVIVSNEEERRSMIEEIGEDVLPVEYGGKAKLIAIQDVVLPHLD 240

Query: 262 G 263
           G
Sbjct: 241 G 240

BLAST of Spg028476 vs. ExPASy Swiss-Prot
Match: P24280 (SEC14 cytosolic factor OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SEC14 PE=1 SV=3)

HSP 1 Score: 85.1 bits (209), Expect = 1.3e-15
Identity = 69/250 (27.60%), Postives = 127/250 (50.80%), Query Frame = 0

Query: 20  GEMENTQQ--ISQMRRLVEKNGVSTEKYGDPTMMRFLIARSMDVEKAAKMFVQWQKWR-- 79
           G +++ Q+  ++++R+L+E  G   E+  D T++RFL AR  DV+ A +MF   +KWR  
Sbjct: 27  GNLDSAQEKALAELRKLLEDAGF-IERLDDSTLLRFLRARKFDVQLAKEMFENCEKWRKD 86

Query: 80  ---DAMVPNGRIDESEI----------EDELGARKIYLQGL-SKNGHSVMIVKGNKHFPA 139
              D ++ +   DE  +          + +   R +Y + L + N H +  V   +    
Sbjct: 87  YGTDTILQDFHYDEKPLIAKFYPQYYHKTDKDGRPVYFEELGAVNLHEMNKVTSEERMLK 146

Query: 140 KDQVQFKKYVAFSLDKTISSAFQGREIGNEKLIGILDLQQISYKN-IDPRGLITGFQFL- 199
               +++  V + L     +A  G  +  E    I+DL+ IS  +       +    ++ 
Sbjct: 147 NLVWEYESVVQYRLPACSRAA--GHLV--ETSCTIMDLKGISISSAYSVMSYVREASYIS 206

Query: 200 QAYYPERLAKCYILNMPRFFVSVWRMVSRFLEKVTLEKIVIVTNEEERRSMIEEIGEEVL 250
           Q YYPER+ K YI+N P  F + +R+   FL+ VT+ KI I+     ++ ++++I  E L
Sbjct: 207 QNYYPERMGKFYIINAPFGFSTAFRLFKPFLDPVTVSKIFIL-GSSYQKELLKQIPAENL 266

BLAST of Spg028476 vs. ExPASy Swiss-Prot
Match: P33324 (CRAL-TRIO domain-containing protein YKL091C OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YKL091C PE=1 SV=2)

HSP 1 Score: 80.9 bits (198), Expect = 2.4e-14
Identity = 72/248 (29.03%), Postives = 121/248 (48.79%), Query Frame = 0

Query: 23  ENTQQISQMRR-LVEKNGVSTEKYGDPTMMRFLIARSMDVEKAAKMFVQWQKWRDAMVPN 82
           E  + + Q R  L+EKN    E+  D T++RFL AR  D+  + +MFV+ ++WR+    N
Sbjct: 28  EQEEALLQFRSILLEKN--YKERLDDSTLLRFLRARKFDINASVEMFVETERWREEYGAN 87

Query: 83  GRID------ESEIEDELGARKIYLQ---GLSKNGHSVMI-----VKGNKHFPAKDQVQF 142
             I+      E+E ++ +   K+Y Q    + K+G  +       +   K +    + Q 
Sbjct: 88  TIIEDYENNKEAEDKERIKLAKMYPQYYHHVDKDGRPLYFEELGGINLKKMYKITTEKQM 147

Query: 143 KKYVA--FSLDKTISSAFQGREIGN--EKLIGILDLQQISYKNIDP--RGLITGFQFLQA 202
            + +   + L  T       R  G   E    +LDL+ IS  N       +       Q 
Sbjct: 148 LRNLVKEYELFATYRVPACSRRAGYLIETSCTVLDLKGISLSNAYHVLSYIKDVADISQN 207

Query: 203 YYPERLAKCYILNMPRFFVSVWRMVSRFLEKVTLEKIVIVTNEEERRSMIEEIGEEVLPV 250
           YYPER+ K YI++ P  F ++++MV  FL+ VT+ KI I+     ++ ++++I  E LPV
Sbjct: 208 YYPERMGKFYIIHSPFGFSTMFKMVKPFLDPVTVSKIFIL-GSSYKKELLKQIPIENLPV 267

BLAST of Spg028476 vs. ExPASy Swiss-Prot
Match: Q10137 (Sec14 cytosolic factor OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=sec14 PE=4 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 7.0e-14
Identity = 63/252 (25.00%), Postives = 120/252 (47.62%), Query Frame = 0

Query: 20  GEMENTQQ--ISQMRRLVEKNGVSTEKYGDPTMMRFLIARSMDVEKAAKMFVQWQKWR-- 79
           G + +TQQ  +  MR  ++K G  TE+  D T++RFL AR  +++++ +MF++ +KWR  
Sbjct: 22  GHLNSTQQATLDSMRLELQKLGY-TERLDDATLLRFLRARKFNLQQSLEMFIKCEKWRKE 81

Query: 80  ---DAMVPNGRIDESEI----------EDELGARKIYLQGLSKNGHSVMIVKGNKHFPAK 139
              D ++ N   DE E           + ++  R +Y++ L         +   K +   
Sbjct: 82  FGVDDLIKNFHYDEKEAVSKYYPQFYHKTDIDGRPVYVEQLGN-------IDLKKLYQIT 141

Query: 140 DQVQFKKYVAFSLDKTISSAFQ--GREIGN--EKLIGILDLQQISYKNIDP--RGLITGF 199
              +  + + +  +      F    R+ G   E    I+DL+ +   +I      +    
Sbjct: 142 TPERMMQNLVYEYEMLALKRFPACSRKAGGLIETSCTIMDLKGVGITSIHSVYSYIRQAS 201

Query: 200 QFLQAYYPERLAKCYILNMPRFFVSVWRMVSRFLEKVTLEKIVIVTNEEERRSMIEEIGE 249
              Q YYPER+ K Y++N P  F S + ++  FL++ T++KI I+     + +++E+I  
Sbjct: 202 SISQDYYPERMGKFYVINAPWGFSSAFNLIKGFLDEATVKKIHIL-GSNYKSALLEQIPA 261

BLAST of Spg028476 vs. ExPASy Swiss-Prot
Match: Q75DK1 (SEC14 cytosolic factor OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=SEC14 PE=3 SV=2)

HSP 1 Score: 77.8 bits (190), Expect = 2.0e-13
Identity = 69/243 (28.40%), Postives = 119/243 (48.97%), Query Frame = 0

Query: 23  ENTQQISQMRRLVEKNGVSTEKYGDPTMMRFLIARSMDVEKAAKMFVQWQKWRDAMVPNG 82
           E+   + ++R+++++ G  T++  D T++RFL AR  DV  A  MF   +KWR     + 
Sbjct: 30  EHEAALEELRKVLKQAGF-TKRLDDSTLLRFLRARKFDVAAARAMFENCEKWRKENGVDT 89

Query: 83  RIDESEIEDELGARKIYLQ---GLSKNGHSVMI-----VKGNKHFPAKDQVQFKK----- 142
             ++   E++    K Y Q      K+G  V I     V   + +    Q +  K     
Sbjct: 90  IFEDFHYEEKPLVAKFYPQYYHKTDKDGRPVYIEELGAVNLTEMYKITTQERMLKNLIWE 149

Query: 143 YVAFSLDKTISSAFQGREIGNEKLIGILDLQQISYKNIDPRGLITGF-----QFLQAYYP 202
           Y +FS  +  +S+ Q  +   E    ILDL+ IS   I     +  +        Q YYP
Sbjct: 150 YESFSRYRLPASSRQA-DCLVETSCTILDLKGIS---ISAAAQVLSYVREASNIGQNYYP 209

Query: 203 ERLAKCYILNMPRFFVSVWRMVSRFLEKVTLEKIVIVTNEEERRSMIEEIGEEVLPVEYG 248
           ER+ K Y++N P  F + +R+   FL+ VT+ KI I+     ++ ++++I  E LPV++G
Sbjct: 210 ERMGKFYMINAPFGFSAAFRLFKPFLDPVTVSKIFIL-GSSYQKELLKQIPAENLPVKFG 266

BLAST of Spg028476 vs. ExPASy Swiss-Prot
Match: P45816 (SEC14 cytosolic factor OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=SEC14 PE=3 SV=2)

HSP 1 Score: 75.5 bits (184), Expect = 1.0e-12
Identity = 62/243 (25.51%), Postives = 121/243 (49.79%), Query Frame = 0

Query: 23  ENTQQISQMRRLVEKNGVSTEKYGDPTMMRFLIARSMDVEKAAKMFVQWQKWRDAMVPNG 82
           E  Q++ +++ ++   G   ++  D T++RFL AR  DV  A +M+   +KWR     N 
Sbjct: 31  EQEQKLGELKMILLTKGYE-DRTDDATLLRFLRARKFDVPLAQEMWENCEKWRKEFGTNT 90

Query: 83  RIDESEIEDELGARKIYLQ---GLSKNGHSVMI-----VKGNKHFPAKDQVQFKKYVAFS 142
            +++   +++    K+Y Q      K+G  V +     V  ++ +    Q +  + + + 
Sbjct: 91  ILEDFWYKEKKEVAKLYPQYYHKTDKDGRPVYVENVGKVNIHEMYKITTQERMLRNLVWE 150

Query: 143 LDKTISSAFQ--GREIGN--EKLIGILDLQQISYKNIDPRGLITGF-----QFLQAYYPE 202
            +  +        R +G+  E    ILDL+ +S   +     + GF        Q YYPE
Sbjct: 151 YESFVRHRLPACSRVVGHLIETSCTILDLKGVS---LSSASQVYGFLKDASNIGQNYYPE 210

Query: 203 RLAKCYILNMPRFFVSVWRMVSRFLEKVTLEKI-VIVTNEEERRSMIEEIGEEVLPVEYG 248
           R+ K Y++N P  F +V+ ++ RFL+ VT+ KI V  +N +E+  ++ ++    LP+++G
Sbjct: 211 RMGKFYLINAPFGFSTVFSVIKRFLDPVTVSKIHVYGSNYKEK--LLAQVPAYNLPIKFG 267

BLAST of Spg028476 vs. ExPASy TrEMBL
Match: A0A6J1JN82 (CRAL-TRIO domain-containing protein YKL091C isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111486211 PE=4 SV=1)

HSP 1 Score: 445.3 bits (1144), Expect = 1.8e-121
Identity = 222/241 (92.12%), Postives = 233/241 (96.68%), Query Frame = 0

Query: 22  MENTQQISQMRRLVEKNGVSTEKYGDPTMMRFLIARSMDVEKAAKMFVQWQKWRDAMVPN 81
           MENTQ+I QMRR+VEKNGVSTEKYGD TMMRFLIARSMDVEKAAKMFVQWQKWRD MVPN
Sbjct: 1   MENTQRILQMRRVVEKNGVSTEKYGDLTMMRFLIARSMDVEKAAKMFVQWQKWRDTMVPN 60

Query: 82  GRIDESEIEDELGARKIYLQGLSKNGHSVMIVKGNKHFPAKDQVQFKKYVAFSLDKTISS 141
           GRIDESEIEDEL ARKIYLQGLS+NGHSVMIVKG+KHFPAKDQVQFKKYV ++LDKTISS
Sbjct: 61  GRIDESEIEDELEARKIYLQGLSQNGHSVMIVKGSKHFPAKDQVQFKKYVVYALDKTISS 120

Query: 142 AFQGREIGNEKLIGILDLQQISYKNIDPRGLITGFQFLQAYYPERLAKCYILNMPRFFVS 201
           AFQGREIGNEKLIGILDLQQISYKNIDPRGLITGFQFLQAYYPERLAKCYILNMPRFF+S
Sbjct: 121 AFQGREIGNEKLIGILDLQQISYKNIDPRGLITGFQFLQAYYPERLAKCYILNMPRFFIS 180

Query: 202 VWRMVSRFLEKVTLEKIVIVTNEEERRSMIEEIGEEVLPVEYGGKAKLIALQDAVLPQLE 261
           VW+MVSRFLEKVTLEKIVIV+NEEER+SMIEEIGEEVLP EYGGK KLIALQDAVLP L+
Sbjct: 181 VWKMVSRFLEKVTLEKIVIVSNEEERKSMIEEIGEEVLPAEYGGKGKLIALQDAVLPHLD 240

Query: 262 G 263
           G
Sbjct: 241 G 241

BLAST of Spg028476 vs. ExPASy TrEMBL
Match: A0A6J1FXA2 (CRAL-TRIO domain-containing protein YKL091C isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111447885 PE=4 SV=1)

HSP 1 Score: 445.3 bits (1144), Expect = 1.8e-121
Identity = 222/241 (92.12%), Postives = 233/241 (96.68%), Query Frame = 0

Query: 22  MENTQQISQMRRLVEKNGVSTEKYGDPTMMRFLIARSMDVEKAAKMFVQWQKWRDAMVPN 81
           MEN+Q+I QMRR+VEKNGVSTEKYGD TMMRFLIARSMDVEKAAKMFVQWQKWRD MVPN
Sbjct: 1   MENSQRILQMRRVVEKNGVSTEKYGDLTMMRFLIARSMDVEKAAKMFVQWQKWRDTMVPN 60

Query: 82  GRIDESEIEDELGARKIYLQGLSKNGHSVMIVKGNKHFPAKDQVQFKKYVAFSLDKTISS 141
           GRIDESEIEDEL ARKIYLQGLS+NGHSVMIVKG+KHFPAKDQVQFKKYV ++LDKTISS
Sbjct: 61  GRIDESEIEDELEARKIYLQGLSRNGHSVMIVKGSKHFPAKDQVQFKKYVVYALDKTISS 120

Query: 142 AFQGREIGNEKLIGILDLQQISYKNIDPRGLITGFQFLQAYYPERLAKCYILNMPRFFVS 201
           AFQGREIGNEKLIGILDLQQISYKNIDPRGLITGFQFLQAYYPERLAKCYILNMPRFF+S
Sbjct: 121 AFQGREIGNEKLIGILDLQQISYKNIDPRGLITGFQFLQAYYPERLAKCYILNMPRFFIS 180

Query: 202 VWRMVSRFLEKVTLEKIVIVTNEEERRSMIEEIGEEVLPVEYGGKAKLIALQDAVLPQLE 261
           VWRMVSRFLEKVTLEKIVIV+NEEER+SMIEEIGEEVLP EYGGK KLIALQDAVLP L+
Sbjct: 181 VWRMVSRFLEKVTLEKIVIVSNEEERKSMIEEIGEEVLPAEYGGKGKLIALQDAVLPHLD 240

Query: 262 G 263
           G
Sbjct: 241 G 241

BLAST of Spg028476 vs. ExPASy TrEMBL
Match: A0A0A0KIQ9 (CRAL-TRIO domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G021330 PE=4 SV=1)

HSP 1 Score: 431.4 bits (1108), Expect = 2.7e-117
Identity = 208/239 (87.03%), Postives = 229/239 (95.82%), Query Frame = 0

Query: 22  MENTQQISQMRRLVEKNGVSTEKYGDPTMMRFLIARSMDVEKAAKMFVQWQKWRDAMVPN 81
           M+NTQ+I Q+RRLVEKNG+STEKYGDP MMRFLIARSMDV+KAAKMFVQWQKWRD MVPN
Sbjct: 1   MDNTQRIEQLRRLVEKNGISTEKYGDPMMMRFLIARSMDVDKAAKMFVQWQKWRDTMVPN 60

Query: 82  GRIDESEIEDELGARKIYLQGLSKNGHSVMIVKGNKHFPAKDQVQFKKYVAFSLDKTISS 141
           G+IDESEIEDELG RK++LQGLSKNGH+V+ +KG+KHFPAKDQVQFKKYV +SLDKTISS
Sbjct: 61  GQIDESEIEDELGTRKMFLQGLSKNGHAVLFLKGSKHFPAKDQVQFKKYVVYSLDKTISS 120

Query: 142 AFQGREIGNEKLIGILDLQQISYKNIDPRGLITGFQFLQAYYPERLAKCYILNMPRFFVS 201
           AF+GREIGNEKLIGILDLQQISYKNIDPRGLITGFQ LQAYYPERLAKCYILNMPRFFVS
Sbjct: 121 AFKGREIGNEKLIGILDLQQISYKNIDPRGLITGFQLLQAYYPERLAKCYILNMPRFFVS 180

Query: 202 VWRMVSRFLEKVTLEKIVIVTNEEERRSMIEEIGEEVLPVEYGGKAKLIALQDAVLPQL 261
           VWRMVSRFLEK TLEK+VIVTNEEERR ++EE+GE+VLPVE+GGKAKLIA+QD VLPQL
Sbjct: 181 VWRMVSRFLEKATLEKVVIVTNEEERRGVVEEVGEDVLPVEFGGKAKLIAIQDVVLPQL 239

BLAST of Spg028476 vs. ExPASy TrEMBL
Match: A0A6J1CYF1 (SEC14 cytosolic factor OS=Momordica charantia OX=3673 GN=LOC111015932 PE=4 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 2.8e-114
Identity = 213/242 (88.02%), Postives = 227/242 (93.80%), Query Frame = 0

Query: 22  MENTQQI--SQMRRLVEKNGVSTEKYGDPTMMRFLIARSMDVEKAAKMFVQWQKWRDAMV 81
           MENTQQI  +QMRRLVE++GV TEKYG+PTMMRFLIARSMD EKAAKMFVQWQKWRD  V
Sbjct: 1   MENTQQIVVAQMRRLVEEHGVPTEKYGEPTMMRFLIARSMDPEKAAKMFVQWQKWRDTTV 60

Query: 82  PNGRIDESEIEDELGARKIYLQGLSKNGHSVMIVKGNKHFPAKDQVQFKKYVAFSLDKTI 141
           PNGRIDESEI+DEL ARKIYLQGLSKNGHSVMIVKGNKH+PAKDQVQFKKYVA+SLDK I
Sbjct: 61  PNGRIDESEIKDELEARKIYLQGLSKNGHSVMIVKGNKHYPAKDQVQFKKYVAYSLDKII 120

Query: 142 SSAFQGREIGNEKLIGILDLQQISYKNIDPRGLITGFQFLQAYYPERLAKCYILNMPRFF 201
           +SAFQGREIGNEKLI ILDLQQ+SYKNIDPRGLITGFQ LQAYYPERLAKCY L+MPRFF
Sbjct: 121 ASAFQGREIGNEKLIAILDLQQLSYKNIDPRGLITGFQSLQAYYPERLAKCYFLHMPRFF 180

Query: 202 VSVWRMVSRFLEKVTLEKIVIVTNEEERRSMIEEIGEEVLPVEYGGKAKLIALQDAVLPQ 261
           VSVWRMVSRFLEK TLEKIVIV+ EEERRS+IEEIGEEVLPVEYGG+AKLIALQD VLP 
Sbjct: 181 VSVWRMVSRFLEKATLEKIVIVSCEEERRSIIEEIGEEVLPVEYGGRAKLIALQDVVLPH 240

BLAST of Spg028476 vs. ExPASy TrEMBL
Match: A0A5A7UTG6 (SEC14 cytosolic factor isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold486G001230 PE=4 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 3.5e-109
Identity = 203/240 (84.58%), Postives = 220/240 (91.67%), Query Frame = 0

Query: 22  MENTQQISQMRRLVEKNGVSTEKYGDPTMMRFLIARSMDVEKAAKMFVQWQKWRDAMVPN 81
           ME+TQ+I QMRRLVEKNG+STE YGDPTMMRFLIARSMDVEKAAKMFVQWQKWRD MVPN
Sbjct: 1   MESTQRIEQMRRLVEKNGISTE-YGDPTMMRFLIARSMDVEKAAKMFVQWQKWRDTMVPN 60

Query: 82  GRIDESEIEDELGARKIYLQGLSKNGHSVMIVKGNKHFPAKDQVQFKK-YVAFSLDKTIS 141
           GRIDESEIEDELG +K++LQGLSKNGH+V+ +KG+KHFPAKDQVQFK     F +     
Sbjct: 61  GRIDESEIEDELGTKKMFLQGLSKNGHAVLFLKGSKHFPAKDQVQFKSDNSLFGVSGVCF 120

Query: 142 SAFQGREIGNEKLIGILDLQQISYKNIDPRGLITGFQFLQAYYPERLAKCYILNMPRFFV 201
           SAF+GREIGNEKLIGILDLQQISYKNIDPRGLITGFQ LQAYYPERLAKCYILNMPRFFV
Sbjct: 121 SAFKGREIGNEKLIGILDLQQISYKNIDPRGLITGFQLLQAYYPERLAKCYILNMPRFFV 180

Query: 202 SVWRMVSRFLEKVTLEKIVIVTNEEERRSMIEEIGEEVLPVEYGGKAKLIALQDAVLPQL 261
           SVWRMVSRFLEK TLEK+VIVT+EEERR MIEEIGE+VLPVE+GGKAKLIA+QD VLPQL
Sbjct: 181 SVWRMVSRFLEKATLEKVVIVTSEEERRGMIEEIGEDVLPVEFGGKAKLIAIQDVVLPQL 239

BLAST of Spg028476 vs. TAIR 10
Match: AT1G14820.3 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 300.1 bits (767), Expect = 1.8e-81
Identity = 149/241 (61.83%), Postives = 189/241 (78.42%), Query Frame = 0

Query: 22  MENTQQ--ISQMRRLVEKNGVSTEKYGDPTMMRFLIARSMDVEKAAKMFVQWQKWRDAMV 81
           ME +Q+  ++Q+R+ VEK   STE Y  PT+MRFL+ARSMD  KAAKMFV WQKWR +MV
Sbjct: 1   MEESQELALTQLRKSVEKLSSSTEGYDKPTLMRFLVARSMDPVKAAKMFVDWQKWRASMV 60

Query: 82  -PNGRIDESEIEDELGARKIYLQGLSKNGHSVMIVKGNKHFPAKDQVQFKKYVAFSLDKT 141
            P G I ESE++DEL  RK+ LQG +K+GH +++V  +KHF +KD   FKK+V ++LDKT
Sbjct: 61  PPTGFIPESEVQDELEFRKVCLQGPTKSGHPLVLVITSKHFASKDPANFKKFVVYALDKT 120

Query: 142 ISSAFQGREIGNEKLIGILDLQQISYKNIDPRGLITGFQFLQAYYPERLAKCYILNMPRF 201
           I+S   G+E+G EKL+ ++DL  I+YKN+D RGLITGFQFLQ+YYPERLAKCYIL+MP F
Sbjct: 121 IASGNNGKEVGGEKLVAVIDLANITYKNLDARGLITGFQFLQSYYPERLAKCYILHMPGF 180

Query: 202 FVSVWRMVSRFLEKVTLEKIVIVTNEEERRSMIEEIGEEVLPVEYGGKAKLIALQDAVLP 260
           FV+VW+ V RFLEK T EKIVIVT+ EE+R   EEIG + LP EYGG+AKL A+QD +LP
Sbjct: 181 FVTVWKFVCRFLEKATQEKIVIVTDGEEQRKFEEEIGADALPEEYGGRAKLTAIQDVLLP 240

BLAST of Spg028476 vs. TAIR 10
Match: AT1G14820.2 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 287.3 bits (734), Expect = 1.2e-77
Identity = 142/222 (63.96%), Postives = 177/222 (79.73%), Query Frame = 0

Query: 40  VSTEK-YGDPTMMRFLIARSMDVEKAAKMFVQWQKWRDAMV-PNGRIDESEIEDELGARK 99
           VS+EK Y  PT+MRFL+ARSMD  KAAKMFV WQKWR +MV P G I ESE++DEL  RK
Sbjct: 7   VSSEKGYDKPTLMRFLVARSMDPVKAAKMFVDWQKWRASMVPPTGFIPESEVQDELEFRK 66

Query: 100 IYLQGLSKNGHSVMIVKGNKHFPAKDQVQFKKYVAFSLDKTISSAFQGREIGNEKLIGIL 159
           + LQG +K+GH +++V  +KHF +KD   FKK+V ++LDKTI+S   G+E+G EKL+ ++
Sbjct: 67  VCLQGPTKSGHPLVLVITSKHFASKDPANFKKFVVYALDKTIASGNNGKEVGGEKLVAVI 126

Query: 160 DLQQISYKNIDPRGLITGFQFLQAYYPERLAKCYILNMPRFFVSVWRMVSRFLEKVTLEK 219
           DL  I+YKN+D RGLITGFQFLQ+YYPERLAKCYIL+MP FFV+VW+ V RFLEK T EK
Sbjct: 127 DLANITYKNLDARGLITGFQFLQSYYPERLAKCYILHMPGFFVTVWKFVCRFLEKATQEK 186

Query: 220 IVIVTNEEERRSMIEEIGEEVLPVEYGGKAKLIALQDAVLPQ 260
           IVIVT+ EE+R   EEIG + LP EYGG+AKL A+QD +LPQ
Sbjct: 187 IVIVTDGEEQRKFEEEIGADALPEEYGGRAKLTAIQDVLLPQ 228

BLAST of Spg028476 vs. TAIR 10
Match: AT1G14820.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 287.3 bits (734), Expect = 1.2e-77
Identity = 142/222 (63.96%), Postives = 177/222 (79.73%), Query Frame = 0

Query: 40  VSTEK-YGDPTMMRFLIARSMDVEKAAKMFVQWQKWRDAMV-PNGRIDESEIEDELGARK 99
           VS+EK Y  PT+MRFL+ARSMD  KAAKMFV WQKWR +MV P G I ESE++DEL  RK
Sbjct: 7   VSSEKGYDKPTLMRFLVARSMDPVKAAKMFVDWQKWRASMVPPTGFIPESEVQDELEFRK 66

Query: 100 IYLQGLSKNGHSVMIVKGNKHFPAKDQVQFKKYVAFSLDKTISSAFQGREIGNEKLIGIL 159
           + LQG +K+GH +++V  +KHF +KD   FKK+V ++LDKTI+S   G+E+G EKL+ ++
Sbjct: 67  VCLQGPTKSGHPLVLVITSKHFASKDPANFKKFVVYALDKTIASGNNGKEVGGEKLVAVI 126

Query: 160 DLQQISYKNIDPRGLITGFQFLQAYYPERLAKCYILNMPRFFVSVWRMVSRFLEKVTLEK 219
           DL  I+YKN+D RGLITGFQFLQ+YYPERLAKCYIL+MP FFV+VW+ V RFLEK T EK
Sbjct: 127 DLANITYKNLDARGLITGFQFLQSYYPERLAKCYILHMPGFFVTVWKFVCRFLEKATQEK 186

Query: 220 IVIVTNEEERRSMIEEIGEEVLPVEYGGKAKLIALQDAVLPQ 260
           IVIVT+ EE+R   EEIG + LP EYGG+AKL A+QD +LPQ
Sbjct: 187 IVIVTDGEEQRKFEEEIGADALPEEYGGRAKLTAIQDVLLPQ 228

BLAST of Spg028476 vs. TAIR 10
Match: AT1G01630.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 160.6 bits (405), Expect = 1.7e-39
Identity = 85/238 (35.71%), Postives = 143/238 (60.08%), Query Frame = 0

Query: 18  LRGEMENTQQISQMRRLVEKNGVSTEKYGDPTMMRFLIARSMDVEKAAKMFVQWQKWRDA 77
           +  E+E + ++  MR L ++    T++  D  + RFL AR +D+EKA+ MF+ +  W+ +
Sbjct: 22  IEDEIERS-KVGIMRALCDRQDPETKEVDDLMIRRFLRARDLDIEKASTMFLNYLTWKRS 81

Query: 78  MVPNGRIDESEIEDELGARKIYLQGLSKNGHSVMIVKGNKHFPAK-DQVQFKKYVAFSLD 137
           M+P G I E+EI ++L   K+ +QG  K G  + +  GN+H P+K +  +FK++V ++L+
Sbjct: 82  MLPKGHIPEAEIANDLSHNKMCMQGHDKMGRPIAVAIGNRHNPSKGNPDEFKRFVVYTLE 141

Query: 138 KTISSAFQGREIGNEKLIGILDLQQISYKNIDPRGLITGFQFLQAYYPERLAKCYILNMP 197
           K  +   +G+    EK + I DLQ   Y N D RG +     LQ  YPERL K YI++ P
Sbjct: 142 KICARMPRGQ----EKFVAIGDLQGWGYSNCDIRGYLAALSTLQDCYPERLGKLYIVHAP 201

Query: 198 RFFVSVWRMVSRFLEKVTLEKIVIVTNEEERRSMIEEIGEEVLPVEYGGKAKLIALQD 255
             F++ W+++  F++  T +KIV V N++   +++E+I E  LP  YGGK  L+ +Q+
Sbjct: 202 YIFMTAWKVIYPFIDANTKKKIVFVENKKLTPTLLEDIDESQLPDIYGGKLPLVPIQE 254

BLAST of Spg028476 vs. TAIR 10
Match: AT4G08690.2 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 81.3 bits (199), Expect = 1.3e-15
Identity = 66/232 (28.45%), Postives = 107/232 (46.12%), Query Frame = 0

Query: 23  ENTQQISQMRRLVEKNGVSTEKY----GDPTMMRFLIARSMDVEKAAKMFVQWQKWRDAM 82
           E   +I ++R+L+   G   EK      D  ++R+L AR+  V+KA KM  +  KWR   
Sbjct: 18  EEQAKIEEVRKLL---GPLPEKLSSFCSDDAVLRYLRARNWHVKKATKMLKETLKWRVQY 77

Query: 83  VPNGRIDESEIEDELGARKIYLQG-LSKNGHSVMIVKGN--KHFPAKDQVQFKKYVAFSL 142
            P   I   E+  E    KIY    + K G  V+I++ +       K Q+++  Y     
Sbjct: 78  KPE-EICWEEVAGEAETGKIYRSSCVDKLGRPVLIMRPSVENSKSVKGQIRYLVY----- 137

Query: 143 DKTISSAFQGREIGNEKLIGILDLQQISYKNIDPRGLITGFQFLQAYYPERLAKCYILNM 202
              + +A Q    G E+++ ++D    S  N+  R        LQ +YPERLA   + N 
Sbjct: 138 --CMENAVQNLPPGEEQMVWMIDFHGYSLANVSLRTTKETAHVLQEHYPERLAFAVLYNP 197

Query: 203 PRFFVSVWRMVSRFLEKVTLEKIVIVTNEEERRSMI--EEIGEEVLPVEYGG 246
           P+FF   W++   FLE  T  K+  V +++    +I  E    E + + +GG
Sbjct: 198 PKFFEPFWKVARPFLEPKTRNKVKFVYSDDPNTKVIMEENFDMEKMELAFGG 238