Spg028151 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg028151
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionEpoxide hydrolase-like
Locationscaffold2: 45640577 .. 45643240 (-)
RNA-Seq ExpressionSpg028151
SyntenySpg028151
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCAAGCACAGATGCAATCCTCCATTTGTCAACGATCGGTTCCTCCGCCATTAAAGATCCACAGCACAACGGGAGAATTCCAAAGTCTTCAAAACCCATTTCACATTTAAACCACCGCCCATTCATTCAATTTCATTATCAGCTCCATTAAAACAAACCCATTTCTCTGAAACTCGCCATTAACAAAGAACCAAAAACAGAGAGCGCAAAAACAAAATCAACCCATCGCCATGGACACAATCGGGCACAGAACCGTGAGCGTCAATGGCATAAACATGCACGTAGCCGAGAAGGGCGAAGGCCCAGTTGTGCTTCTCATCCATGGCTTCCCAGAGCTTTGGTATACATGGCGCCATCAGATTTTGGCGTTGAGCTCACTTGGGTACCGCGCTGTGGCGCCGGACCTTCGTGGGTATGGCGATTCTGATGCGCCTGGTTCGATTTCTAGCTACTCTATTATGCATATTGTTGGGGATCTTGTTGCTCTTGTTGAGAGTCTTGGAGTTAAGGAGGTTTTTGTTGTGGCCCATGATTGGGGCGCATTGATTGCATGGTGTTTGTGTTTATTCCGAGCTGAGATTGTGAAGGCTTTTGTGTGTCTAAGTGTGCCGTTTAGGCCTAGGCATCCACAGATGAAGCCTGTTGAGAGCATGAGGAAATTTTTTGGAGATGATTATTACATCTGCAGATTTCAGGTTGGGTTCTTTTTGTAATTGCTTGGTGCTTGTTGAAAATGTTGGGGTTTGATTGTGCTTTTGCCATGGTGATTAGAATGCACCAAAAACACTTCACACCCTCAAAATGAGTTCTTTAAATAGAGTTTGTCTTTGTGCTGCTAGGTATTGTCTACCTACCTATCCATTTGTGTTGATTTTAGGTGCAGGTACACTTTTGTTGAAGGTCATTTGCTGGTTTTTTTAATGTTGGGAAATAGTTGAACAATGAAACTAGATGGTGAGTGCCTATTGATAGTGGGTAGTCCTACAAGATGCTGTACCCACCTATGGTTATGGAACTGAATTCATTAGCATGGACCATTTTCTTTTGATCATCTTAACCCATGTTTGGAAGTGATCTTGGCATGAGTTTGGAACAAATTTTGAAACAAGAGTAAATGGTTTTGACCAACAAAAAATTGCTTTTCAAACTTTTCATAGTGTTTGGTTGAACAATCGATAGTGATTTTGAAAATGATAAGTATAGAATGCACTTCTTCAGAACTATGGACTTGAATCAGAGGTCTTAAGGTCGAAGGTCGAGTCTGTGAACCACTCTTGTGGTTTGGCCAATAGTGGGCCAAGATATATTGAATTGCACACCAGAAGATGACCTTCTTTTTGTACTTTGTGTTTGGGAACAGAGCTATGCCTATCGATCATAAGAAAATTTTATGTGTTTCAGAATCCAGGAGAGATTGAAGAAGAGATGGCCCAGGTAGGGGCCAAAGAAGTTCTAAAAGGCATATTGACAACAAGAACACCAGGGCCTCCCATTTTGCCCAAAGGAAAAGCCTTCAGACCCAGGCCTGGATCCTCAACTGCCTTGCCCTCTTGGCTTTCAGAAGAAGATCTTTCTTACTTTGCCTCCAAATATGAGCAGAAGGGCTTCACTGGACCATTGAACTATTACAGATCCATGGATTTGTAAGTCACCAACTACAAAACTTCTGATTTAAACAAAATAGAATCCCAAGTTTTGCTTATCTGTCTGTGGTTAAGTTACATTTTTAAGTCTCATATTTAACTTTAGTTTTAAAATTTTCACTATCATCCTTTTATCTTCATATTGCTCACTCTAAGATATCATTGGTTTCACTCTGAAACAAGATCAACCTTTTAACTCTCTTTTACTTTTCAACAACAATAATGCCTTGAAATCTAACCAAAAACCATGTTTTAACTTCTAAATCATCATGAAGCTCATCTGCGTTCTTGGTTTCAAATTTTCTCGAGTTTATCTAGGAGTTAGGACCTCATTGGCACAGTAAGATCTCATAGATTGTACTTTCTCTAAGCTAGATTTTTGTTACATCTTGAAAACCTCAATCGAACAATAGGGATAGTAATAGAAACGCTTAAAAGTATAGGGACCAAATTGACACAGAACGTTTAAGTATTTGGATTTTGGTGCAGAAACTGGGAGCTGACAGCACCATGGACAGGGGTGCAAGTGAAAGTGCCAGTGAAGTTCATAGTTGGAGAAGTTGACATGGTGTATACAACACCAGGGGTTAAGGAGTATGTCAATGGTGGAGGTTTCAAGAAAGATGTGCCATTTTTGCAAGAAGTTGTGGTAATGGAGGGAGTTGGCCATTTCCTCAACCAGGAAAAGCCTGAAGAGATCAGCACTCACATCCATGACTTCATCAAAAAATTCTAGTCTCATTCTACAAAGATTATACAAAGTTCAAGTCTCCCAAAACACATGGTGTGTGTTTCAACTTATGCTGTCTGTTTGTTTCTTCTAAATGATTTGAATGTCTTGGAATCTAGATGGCTGTTTGGAAGTTACTTTTTAGAATATGTTTGGTGTATGAAACTTTATGGTGCTGTATAAAAGTTGTTCTTAGATATGGAATAAAAAGTGGATGAAAATATGTATGGTCTTTTATTATTTTTCATGGACAGTTTGTTTTTGAGATTAATAGAAGTTGTTCCTCCTTGG

mRNA sequence

ATGGACACAATCGGGCACAGAACCGTGAGCGTCAATGGCATAAACATGCACGTAGCCGAGAAGGGCGAAGGCCCAGTTGTGCTTCTCATCCATGGCTTCCCAGAGCTTTGGTATACATGGCGCCATCAGATTTTGGCGTTGAGCTCACTTGGGTACCGCGCTGTGGCGCCGGACCTTCGTGGGTATGGCGATTCTGATGCGCCTGGTTCGATTTCTAGCTACTCTATTATGCATATTGTTGGGGATCTTGTTGCTCTTGTTGAGAGTCTTGGAGTTAAGGAGGTTTTTGTTGTGGCCCATGATTGGGGCGCATTGATTGCATGGTGTTTGTGTTTATTCCGAGCTGAGATTGTGAAGGCTTTTGTGTGTCTAAGTGTGCCGTTTAGGCCTAGGCATCCACAGATGAAGCCTGTTGAGAGCATGAGGAAATTTTTTGGAGATGATTATTACATCTGCAGATTTCAGAATCCAGGAGAGATTGAAGAAGAGATGGCCCAGGTAGGGGCCAAAGAAGTTCTAAAAGGCATATTGACAACAAGAACACCAGGGCCTCCCATTTTGCCCAAAGGAAAAGCCTTCAGACCCAGGCCTGGATCCTCAACTGCCTTGCCCTCTTGGCTTTCAGAAGAAGATCTTTCTTACTTTGCCTCCAAATATGAGCAGAAGGGCTTCACTGGACCATTGAACTATTACAGATCCATGGATTTAAACTGGGAGCTGACAGCACCATGGACAGGGGTGCAAGTGAAAGTGCCAGTGAAGTTCATAGTTGGAGAAGTTGACATGGTGTATACAACACCAGGGGTTAAGGAGTATGTCAATGGTGGAGGTTTCAAGAAAGATGTGCCATTTTTGCAAGAAGTTGTGGTAATGGAGGGAGTTGGCCATTTCCTCAACCAGGAAAAGCCTGAAGAGATCAGCACTCACATCCATGACTTCATCAAAAAATTCTAG

Coding sequence (CDS)

ATGGACACAATCGGGCACAGAACCGTGAGCGTCAATGGCATAAACATGCACGTAGCCGAGAAGGGCGAAGGCCCAGTTGTGCTTCTCATCCATGGCTTCCCAGAGCTTTGGTATACATGGCGCCATCAGATTTTGGCGTTGAGCTCACTTGGGTACCGCGCTGTGGCGCCGGACCTTCGTGGGTATGGCGATTCTGATGCGCCTGGTTCGATTTCTAGCTACTCTATTATGCATATTGTTGGGGATCTTGTTGCTCTTGTTGAGAGTCTTGGAGTTAAGGAGGTTTTTGTTGTGGCCCATGATTGGGGCGCATTGATTGCATGGTGTTTGTGTTTATTCCGAGCTGAGATTGTGAAGGCTTTTGTGTGTCTAAGTGTGCCGTTTAGGCCTAGGCATCCACAGATGAAGCCTGTTGAGAGCATGAGGAAATTTTTTGGAGATGATTATTACATCTGCAGATTTCAGAATCCAGGAGAGATTGAAGAAGAGATGGCCCAGGTAGGGGCCAAAGAAGTTCTAAAAGGCATATTGACAACAAGAACACCAGGGCCTCCCATTTTGCCCAAAGGAAAAGCCTTCAGACCCAGGCCTGGATCCTCAACTGCCTTGCCCTCTTGGCTTTCAGAAGAAGATCTTTCTTACTTTGCCTCCAAATATGAGCAGAAGGGCTTCACTGGACCATTGAACTATTACAGATCCATGGATTTAAACTGGGAGCTGACAGCACCATGGACAGGGGTGCAAGTGAAAGTGCCAGTGAAGTTCATAGTTGGAGAAGTTGACATGGTGTATACAACACCAGGGGTTAAGGAGTATGTCAATGGTGGAGGTTTCAAGAAAGATGTGCCATTTTTGCAAGAAGTTGTGGTAATGGAGGGAGTTGGCCATTTCCTCAACCAGGAAAAGCCTGAAGAGATCAGCACTCACATCCATGACTTCATCAAAAAATTCTAG

Protein sequence

MDTIGHRTVSVNGINMHVAEKGEGPVVLLIHGFPELWYTWRHQILALSSLGYRAVAPDLRGYGDSDAPGSISSYSIMHIVGDLVALVESLGVKEVFVVAHDWGALIAWCLCLFRAEIVKAFVCLSVPFRPRHPQMKPVESMRKFFGDDYYICRFQNPGEIEEEMAQVGAKEVLKGILTTRTPGPPILPKGKAFRPRPGSSTALPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWELTAPWTGVQVKVPVKFIVGEVDMVYTTPGVKEYVNGGGFKKDVPFLQEVVVMEGVGHFLNQEKPEEISTHIHDFIKKF
Homology
BLAST of Spg028151 vs. NCBI nr
Match: XP_038879653.1 (epoxide hydrolase A-like [Benincasa hispida])

HSP 1 Score: 633.3 bits (1632), Expect = 1.2e-177
Identity = 300/317 (94.64%), Postives = 309/317 (97.48%), Query Frame = 0

Query: 1   MDTIGHRTVSVNGINMHVAEKGEGPVVLLIHGFPELWYTWRHQILALSSLGYRAVAPDLR 60
           MDTI HRTVSVNGINMH+AEKGEGP+VLLIHGFPELWY WRHQILALSSLGYRAVAPDLR
Sbjct: 1   MDTIEHRTVSVNGINMHIAEKGEGPIVLLIHGFPELWYAWRHQILALSSLGYRAVAPDLR 60

Query: 61  GYGDSDAPGSISSYSIMHIVGDLVALVESLGVKEVFVVAHDWGALIAWCLCLFRAEIVKA 120
           GYGDSDAP SISSYSIMHIVGDLVALVESLGVKEVFVVAHDWGALIAWCLCLFRAEIVKA
Sbjct: 61  GYGDSDAPDSISSYSIMHIVGDLVALVESLGVKEVFVVAHDWGALIAWCLCLFRAEIVKA 120

Query: 121 FVCLSVPFRPRHPQMKPVESMRKFFGDDYYICRFQNPGEIEEEMAQVGAKEVLKGILTTR 180
           FVCLSVPFRPRHP MKPVE+MRKFFGDDYYICRFQNPGEIEEEMAQVGAKEVLKGILTTR
Sbjct: 121 FVCLSVPFRPRHPYMKPVETMRKFFGDDYYICRFQNPGEIEEEMAQVGAKEVLKGILTTR 180

Query: 181 TPGPPILPKGKAFRPRPGSSTALPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL 240
            PGPPILPKG+AFR RPGSS+ LPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL
Sbjct: 181 RPGPPILPKGQAFRARPGSSSPLPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL 240

Query: 241 TAPWTGVQVKVPVKFIVGEVDMVYTTPGVKEYVNGGGFKKDVPFLQEVVVMEGVGHFLNQ 300
           TAPWTGVQVKVPVKFIVG+ DMVYTTPG+KEYVNGGGFKKDVPFLQEVV+MEGVGHFLNQ
Sbjct: 241 TAPWTGVQVKVPVKFIVGDADMVYTTPGIKEYVNGGGFKKDVPFLQEVVIMEGVGHFLNQ 300

Query: 301 EKPEEISTHIHDFIKKF 318
           EKPEEISTHIHDFI+KF
Sbjct: 301 EKPEEISTHIHDFIRKF 317

BLAST of Spg028151 vs. NCBI nr
Match: XP_008450607.1 (PREDICTED: bifunctional epoxide hydrolase 2-like isoform X1 [Cucumis melo] >KAA0050918.1 bifunctional epoxide hydrolase 2-like isoform X1 [Cucumis melo var. makuwa] >TYK10266.1 bifunctional epoxide hydrolase 2-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 606.7 bits (1563), Expect = 1.2e-169
Identity = 289/317 (91.17%), Postives = 302/317 (95.27%), Query Frame = 0

Query: 1   MDTIGHRTVSVNGINMHVAEKGEGPVVLLIHGFPELWYTWRHQILALSSLGYRAVAPDLR 60
           MDTI HRTVSVNGIN+H+AEKGEGP+VL IHGFPELWYTWRHQILALSSLGY AVAPDLR
Sbjct: 1   MDTINHRTVSVNGINVHIAEKGEGPIVLFIHGFPELWYTWRHQILALSSLGYHAVAPDLR 60

Query: 61  GYGDSDAPGSISSYSIMHIVGDLVALVESLGVKEVFVVAHDWGALIAWCLCLFRAEIVKA 120
           GYGDSDAP SISSYSIMHIVGDLVALVESLGVKEVFVVAHDWGALIAW LCLFRAEIVKA
Sbjct: 61  GYGDSDAPDSISSYSIMHIVGDLVALVESLGVKEVFVVAHDWGALIAWGLCLFRAEIVKA 120

Query: 121 FVCLSVPFRPRHPQMKPVESMRKFFGDDYYICRFQNPGEIEEEMAQVGAKEVLKGILTTR 180
           FVCLSVPFRPRHP  KPVE+MRK FGDDYYICRFQNPGEIEEEMAQVGAKEVL+GILTTR
Sbjct: 121 FVCLSVPFRPRHPNRKPVETMRKVFGDDYYICRFQNPGEIEEEMAQVGAKEVLRGILTTR 180

Query: 181 TPGPPILPKGKAFRPRPGSSTALPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL 240
             GPPI PK +AFR RP SS+ LPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL
Sbjct: 181 RQGPPIYPKKQAFRARPESSSPLPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL 240

Query: 241 TAPWTGVQVKVPVKFIVGEVDMVYTTPGVKEYVNGGGFKKDVPFLQEVVVMEGVGHFLNQ 300
           TAPWTGVQVKVPVKFIVG+VDMVYTTPGVKEYVNGGGFKKDVPFLQ+VV+MEGVGHFLNQ
Sbjct: 241 TAPWTGVQVKVPVKFIVGDVDMVYTTPGVKEYVNGGGFKKDVPFLQDVVIMEGVGHFLNQ 300

Query: 301 EKPEEISTHIHDFIKKF 318
           EKPEEI+THI+DFI+KF
Sbjct: 301 EKPEEINTHIYDFIRKF 317

BLAST of Spg028151 vs. NCBI nr
Match: XP_023531428.1 (uncharacterized protein LOC111793669 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 603.2 bits (1554), Expect = 1.3e-168
Identity = 284/317 (89.59%), Postives = 303/317 (95.58%), Query Frame = 0

Query: 1   MDTIGHRTVSVNGINMHVAEKGEGPVVLLIHGFPELWYTWRHQILALSSLGYRAVAPDLR 60
           MD+I HRTVSVNGINMH+AEKGEGP+VLLIHGFPELWY+WRHQILALSS GYRAVAPDLR
Sbjct: 1   MDSIQHRTVSVNGINMHIAEKGEGPIVLLIHGFPELWYSWRHQILALSSQGYRAVAPDLR 60

Query: 61  GYGDSDAPGSISSYSIMHIVGDLVALVESLGVKEVFVVAHDWGALIAWCLCLFRAEIVKA 120
           GYGDSDAP S+SSYSIMHIVGDLVALVE LGVKEVFVVAHDWGA+IAWCLCLFR +IVKA
Sbjct: 61  GYGDSDAPDSLSSYSIMHIVGDLVALVEILGVKEVFVVAHDWGAMIAWCLCLFRPDIVKA 120

Query: 121 FVCLSVPFRPRHPQMKPVESMRKFFGDDYYICRFQNPGEIEEEMAQVGAKEVLKGILTTR 180
           FVCLSVPFRPRHP++KPVESMRKFFGDDYYICRFQNPGEIEEEMA VGAKEV++GILTTR
Sbjct: 121 FVCLSVPFRPRHPKIKPVESMRKFFGDDYYICRFQNPGEIEEEMAVVGAKEVVRGILTTR 180

Query: 181 TPGPPILPKGKAFRPRPGSSTALPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL 240
             GPPI PKG+AF+ RPGSST LPSWLSEEDLSYFASK+E KGFTGPLNYYRSMDLNWEL
Sbjct: 181 KTGPPIFPKGQAFQVRPGSSTPLPSWLSEEDLSYFASKFESKGFTGPLNYYRSMDLNWEL 240

Query: 241 TAPWTGVQVKVPVKFIVGEVDMVYTTPGVKEYVNGGGFKKDVPFLQEVVVMEGVGHFLNQ 300
           TAPWTGVQVKVPVKF+VG+VDMVYTTPGVKEYVNGGGFKKDVP+LQEVVVMEGVGHFL Q
Sbjct: 241 TAPWTGVQVKVPVKFVVGDVDMVYTTPGVKEYVNGGGFKKDVPYLQEVVVMEGVGHFLTQ 300

Query: 301 EKPEEISTHIHDFIKKF 318
           EKPEE+S+HI DFIKKF
Sbjct: 301 EKPEEVSSHILDFIKKF 317

BLAST of Spg028151 vs. NCBI nr
Match: XP_022969836.1 (uncharacterized protein LOC111468917 [Cucurbita maxima])

HSP 1 Score: 603.2 bits (1554), Expect = 1.3e-168
Identity = 284/317 (89.59%), Postives = 303/317 (95.58%), Query Frame = 0

Query: 1   MDTIGHRTVSVNGINMHVAEKGEGPVVLLIHGFPELWYTWRHQILALSSLGYRAVAPDLR 60
           MD+I HRTVSVNGINMH+AEKGEGP+VLLIHGFPELWY+WRHQILALSS GYRAVAPDLR
Sbjct: 1   MDSIQHRTVSVNGINMHIAEKGEGPIVLLIHGFPELWYSWRHQILALSSHGYRAVAPDLR 60

Query: 61  GYGDSDAPGSISSYSIMHIVGDLVALVESLGVKEVFVVAHDWGALIAWCLCLFRAEIVKA 120
           GYGDSDAP S+SSYSIMHIVGDLVALVE LGVKEVFVVAHDWGA+IAWCLCLFRA+IVKA
Sbjct: 61  GYGDSDAPDSLSSYSIMHIVGDLVALVEILGVKEVFVVAHDWGAMIAWCLCLFRADIVKA 120

Query: 121 FVCLSVPFRPRHPQMKPVESMRKFFGDDYYICRFQNPGEIEEEMAQVGAKEVLKGILTTR 180
           FVCLSVPFRPRHP++KPVESMRKFFGDDYYICRFQNPGEIEEEMA VGAKEV++GILTTR
Sbjct: 121 FVCLSVPFRPRHPKVKPVESMRKFFGDDYYICRFQNPGEIEEEMAMVGAKEVVRGILTTR 180

Query: 181 TPGPPILPKGKAFRPRPGSSTALPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL 240
             GPPI PKG+AF+ RP SST LPSWLSEEDLSYFASK+E KGFTGPLNYYRSMDLNWEL
Sbjct: 181 RTGPPIFPKGQAFKSRPESSTPLPSWLSEEDLSYFASKFESKGFTGPLNYYRSMDLNWEL 240

Query: 241 TAPWTGVQVKVPVKFIVGEVDMVYTTPGVKEYVNGGGFKKDVPFLQEVVVMEGVGHFLNQ 300
           TAPWTGVQVKVPVKF+VG+VDMVYTTPGVKEYVNGGGFKKDVP+LQEVVVMEGVGHFL Q
Sbjct: 241 TAPWTGVQVKVPVKFVVGDVDMVYTTPGVKEYVNGGGFKKDVPYLQEVVVMEGVGHFLTQ 300

Query: 301 EKPEEISTHIHDFIKKF 318
           EKPEE+S+HI DFIKKF
Sbjct: 301 EKPEEVSSHILDFIKKF 317

BLAST of Spg028151 vs. NCBI nr
Match: KAG6588564.1 (hypothetical protein SDJN03_17129, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 601.7 bits (1550), Expect = 3.7e-168
Identity = 284/317 (89.59%), Postives = 302/317 (95.27%), Query Frame = 0

Query: 1   MDTIGHRTVSVNGINMHVAEKGEGPVVLLIHGFPELWYTWRHQILALSSLGYRAVAPDLR 60
           MD+I HRTVSVNGINMH+AEKGEGP+VLLIHGFPELWY+WRHQILALSS  YRAVAPDLR
Sbjct: 1   MDSIQHRTVSVNGINMHIAEKGEGPIVLLIHGFPELWYSWRHQILALSSQEYRAVAPDLR 60

Query: 61  GYGDSDAPGSISSYSIMHIVGDLVALVESLGVKEVFVVAHDWGALIAWCLCLFRAEIVKA 120
           GYGDSDAP S+SSYSIMHIVGDLVALVE LGVKEVFVVAHDWGA+IAWCLCLFR +IVKA
Sbjct: 61  GYGDSDAPDSLSSYSIMHIVGDLVALVEILGVKEVFVVAHDWGAMIAWCLCLFRPDIVKA 120

Query: 121 FVCLSVPFRPRHPQMKPVESMRKFFGDDYYICRFQNPGEIEEEMAQVGAKEVLKGILTTR 180
           FVCLSVPFRPRHP++KPVESMRKFFGDDYYICRFQNPGEIEEEMA VGAKEV++GILTTR
Sbjct: 121 FVCLSVPFRPRHPKIKPVESMRKFFGDDYYICRFQNPGEIEEEMAVVGAKEVVRGILTTR 180

Query: 181 TPGPPILPKGKAFRPRPGSSTALPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL 240
             GPPI PKG+AFR RPGSST LPSWLSEEDLSYFASK+E KGFTGPLNYYRSMDLNWEL
Sbjct: 181 KAGPPIFPKGQAFRVRPGSSTPLPSWLSEEDLSYFASKFESKGFTGPLNYYRSMDLNWEL 240

Query: 241 TAPWTGVQVKVPVKFIVGEVDMVYTTPGVKEYVNGGGFKKDVPFLQEVVVMEGVGHFLNQ 300
           TAPWTGVQVKVPVKF+VG+VDMVYTTPGVKEYVNGGGFKKDVP+LQEVVVMEGVGHFL Q
Sbjct: 241 TAPWTGVQVKVPVKFVVGDVDMVYTTPGVKEYVNGGGFKKDVPYLQEVVVMEGVGHFLTQ 300

Query: 301 EKPEEISTHIHDFIKKF 318
           EKPEE+S+HI DFIKKF
Sbjct: 301 EKPEEVSSHILDFIKKF 317

BLAST of Spg028151 vs. ExPASy Swiss-Prot
Match: I6YGS0 (Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=ephA PE=1 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 4.3e-58
Identity = 125/320 (39.06%), Postives = 179/320 (55.94%), Query Frame = 0

Query: 7   RTVSVNGINMHVAEKGE--GPVVLLIHGFPELWYTWRHQILALSSLGYRAVAPDLRGYGD 66
           R V  NG+ + V E GE   PVV+L HGFPEL Y+WRHQI AL+  GY  +APD RGYG 
Sbjct: 7   RLVDTNGVRLRVVEAGEPGAPVVILAHGFPELAYSWRHQIPALADAGYHVLAPDQRGYGG 66

Query: 67  SDAPGSISSYSIMHIVGDLVALVESLGVKEVFVVAHDWGALIAWCLCLFRAEIVKAFVCL 126
           S  P +I +Y I  +  DLV L++ +G +    V HDWGA++ W   L  A+ V A   L
Sbjct: 67  SSRPEAIEAYDIHRLTADLVGLLDDVGAERAVWVGHDWGAVVVWNAPLLHADRVAAVAAL 126

Query: 127 SVPFRPRHPQMKPVESMRKFFGDD-YYICRFQNPGEIEEEMAQVGAKEVLKGILTTRTPG 186
           SVP  PR  Q+ P ++ R  FG++ +YI  FQ PG  + E+    A+ + + I   R PG
Sbjct: 127 SVPALPR-AQVPPTQAFRSRFGENFFYILYFQEPGIADAELNGDPARTMRRMIGGLRPPG 186

Query: 187 PP------ILPKGKAFRPRPGSSTALPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLN 246
                   + P    F  R      LP+W+S+E+L ++  ++ + GFTG LN+YR+ D N
Sbjct: 187 DQSAAMRMLAPGPDGFIDRLPEPAGLPAWISQEELDHYIGEFTRTGFTGGLNWYRNFDRN 246

Query: 247 WELTAPWTGVQVKVPVKFIVGEVDMVYT---TPGVKEYVNGGGFKKDVPFLQEVVVMEGV 306
           WE TA   G  + VP  FI G  D V T   T    E ++G       P+ +  V+++G 
Sbjct: 247 WETTADLAGKTISVPSLFIAGTADPVLTFTRTDRAAEVISG-------PYRE--VLIDGA 306

Query: 307 GHFLNQEKPEEISTHIHDFI 315
           GH+L QE+P E++  + +F+
Sbjct: 307 GHWLQQERPGEVTAALLEFL 316

BLAST of Spg028151 vs. ExPASy Swiss-Prot
Match: P34913 (Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2)

HSP 1 Score: 186.0 bits (471), Expect = 6.4e-46
Identity = 97/298 (32.55%), Postives = 158/298 (53.02%), Query Frame = 0

Query: 14  INMHVAEKGEGPVVLLIHGFPELWYTWRHQILALSSLGYRAVAPDLRGYGDSDAPGSISS 73
           + +H  E G GP V L HGFPE WY+WR+QI AL+  GYR +A D++GYG+S AP  I  
Sbjct: 248 VRLHFVELGSGPAVCLCHGFPESWYSWRYQIPALAQAGYRVLAMDMKGYGESSAPPEIEE 307

Query: 74  YSIMHIVGDLVALVESLGVKEVFVVAHDWGALIAWCLCLFRAEIVKAFVCLSVPFRPRHP 133
           Y +  +  ++V  ++ LG+ +   + HDWG ++ W + LF  E V+A   L+ PF P +P
Sbjct: 308 YCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPERVRAVASLNTPFIPANP 367

Query: 134 QMKPVESMRKFFGDDYYICRFQNPGEIEEEMAQVGAKEVLKGILTTRTPGPPILPK---- 193
            M P+ES++     DY +  FQ PG  E E+ Q       K +          + K    
Sbjct: 368 NMSPLESIKANPVFDYQL-YFQEPGVAEAELEQ-NLSRTFKSLFRASDESVLSMHKVCEA 427

Query: 194 GKAFRPRPGSSTALPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWELTAPWTGVQV 253
           G  F   P    +L   ++EE++ ++  ++++ GF GPLN+YR+M+ NW+      G ++
Sbjct: 428 GGLFVNSP-EEPSLSRMVTEEEIQFYVQQFKKSGFRGPLNWYRNMERNWKWACKSLGRKI 487

Query: 254 KVPVKFIVGEVDMVYTTPGVKEYVNGGGFKKDVPFLQEVVVMEGVGHFLNQEKPEEIS 308
            +P   +  E D V   P + +++        +P L+   + E  GH+   +KP E++
Sbjct: 488 LIPALMVTAEKDFV-LVPQMSQHMEDW-----IPHLKRGHI-EDCGHWTQMDKPTEVN 535

BLAST of Spg028151 vs. ExPASy Swiss-Prot
Match: Q6Q2C2 (Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 4.2e-45
Identity = 97/306 (31.70%), Postives = 162/306 (52.94%), Query Frame = 0

Query: 13  GINMHVAEKGEGPVVLLIHGFPELWYTWRHQILALSSLGYRAVAPDLRGYGDSDAPGSIS 72
           G+ +H  E G GP V L HGFPE W++WR+QI AL+  G+R +A D++GYG+S AP  I 
Sbjct: 247 GVRLHFVEMGSGPAVCLCHGFPESWFSWRYQIPALAQAGFRVLAVDMKGYGESSAPPEIE 306

Query: 73  SYSIMHIVGDLVALVESLGVKEVFVVAHDWGALIAWCLCLFRAEIVKAFVCLSVPFRPRH 132
            YS+  +  D+V  +  LG+ +   + HDWG ++ W + LF  E V+A   L+ PF P +
Sbjct: 307 EYSLEVLCKDMVTFLNKLGLSQAVFIGHDWGGVLVWNMALFYPERVRAVASLNTPFMPSN 366

Query: 133 PQMKPVESMRKFFGDDYYICRFQNPGEIEEEMAQ---VGAKEVLKGILTTRTPGPPILPK 192
           P + P+E ++     DY +  FQ PG  E E+ Q      K   +    T      +   
Sbjct: 367 PNVSPMEIIKANPVFDYQL-YFQEPGVAEAELEQNLDRTFKNFFRAHDETFLTTNRVREL 426

Query: 193 GKAFRPRPGSSTALPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWELTAPWTGVQV 252
           G  F   P    +L   ++EED+ ++  ++++ GF GPLN+YR+M+ NW+     +G ++
Sbjct: 427 GGLFVGTP-EEPSLSRLVTEEDIQFYVQQFKKSGFRGPLNWYRNMERNWQWGCKGSGRKI 486

Query: 253 KVPVKFIVGEVDMVYTTPGVKEYVNGGGFKKDVPFLQEVVVMEGVGHFLNQEKPEEISTH 312
            +P   +  E D+V      K   N       +P L+   + +  GH+   +KP E++  
Sbjct: 487 LIPALMVTAENDLVLHPKMSKHMENW------IPHLKRGHIKD-CGHWTQIDKPAELNRI 543

Query: 313 IHDFIK 316
           + ++++
Sbjct: 547 LIEWLE 543

BLAST of Spg028151 vs. ExPASy Swiss-Prot
Match: P80299 (Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 3.5e-44
Identity = 100/319 (31.35%), Postives = 166/319 (52.04%), Query Frame = 0

Query: 4   IGHRTVSVN-GINMHVAEKGEGPVVLLIHGFPELWYTWRHQILALSSLGYRAVAPDLRGY 63
           + H  V+V  GI +H  E G GP + L HGFPE W++WR+QI AL+  G+R +A D++GY
Sbjct: 235 VSHGYVTVKPGIRLHFVEMGSGPAICLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKGY 294

Query: 64  GDSDAPGSISSYSIMHIVGDLVALVESLGVKEVFVVAHDWGALIAWCLCLFRAEIVKAFV 123
           GDS +P  I  Y++  +  ++V  +  LG+ +   + HDW  ++ W + LF  E V+A  
Sbjct: 295 GDSSSPPEIEEYAMELLCEEMVTFLNKLGIPQAVFIGHDWAGVLVWNMALFHPERVRAVA 354

Query: 124 CLSVPFRPRHPQMKPVESMRKFFGDDYYICRFQNPGEIEEEMAQVGAKEVLKGILTTRTP 183
            L+ P  P +P++ P+E +R     +Y +  FQ PG  E E+ +  ++       T+   
Sbjct: 355 SLNTPLMPPNPEVSPMEVIRSIPVFNYQL-YFQEPGVAEAELEKNMSRTFKSFFRTSDDM 414

Query: 184 GPPILPKGKAFRPRPGSSTALP------SWLSEEDLSYFASKYEQKGFTGPLNYYRSMDL 243
           G  +L   KA     G     P         +EE++ Y+  ++++ GF GPLN+YR+ + 
Sbjct: 415 G--LLTVNKA-TEMGGILVGTPEDPKVSKITTEEEIEYYIQQFKKSGFRGPLNWYRNTER 474

Query: 244 NWELTAPWTGVQVKVPVKFIVGEVDMVYTTPGVKEYVNGGGFKKDVPFLQEVVVMEGVGH 303
           NW+ +    G ++ VP   +  E D+V      K   N       +PFL+   + E  GH
Sbjct: 475 NWKWSCKALGRKILVPALMVTAEKDIVLRPEMSKNMENW------IPFLKRGHI-EDCGH 534

Query: 304 FLNQEKPEEISTHIHDFIK 316
           +   EKP E++  +  ++K
Sbjct: 535 WTQIEKPAEVNQILIKWLK 542

BLAST of Spg028151 vs. ExPASy Swiss-Prot
Match: P34914 (Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2)

HSP 1 Score: 174.9 bits (442), Expect = 1.5e-42
Identity = 94/308 (30.52%), Postives = 159/308 (51.62%), Query Frame = 0

Query: 4   IGHRTVSVN-GINMHVAEKGEGPVVLLIHGFPELWYTWRHQILALSSLGYRAVAPDLRGY 63
           + H  V+V  GI +H  E G GP + L HGFPE W++WR+QI AL+  G+R +A D++GY
Sbjct: 235 VSHGYVTVKPGIRLHFVEMGSGPALCLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKGY 294

Query: 64  GDSDAPGSISSYSIMHIVGDLVALVESLGVKEVFVVAHDWGALIAWCLCLFRAEIVKAFV 123
           GDS +P  I  Y++  +  ++V  ++ LG+ +   + HDW  ++ W + LF  E V+A  
Sbjct: 295 GDSSSPPEIEEYAMELLCKEMVTFLDKLGIPQAVFIGHDWAGVMVWNMALFYPERVRAVA 354

Query: 124 CLSVPFRPRHPQMKPVESMRKFFGDDYYICRFQNPGEIEEEMAQVGAKEVLKGILTTRTP 183
            L+ PF P  P + P++ +R     +Y +  FQ PG  E E+ +  ++        +   
Sbjct: 355 SLNTPFMPPDPDVSPMKVIRSIPVFNYQL-YFQEPGVAEAELEKNMSRTFKSFFRASDET 414

Query: 184 GPPILPKGK---AFRPRPGSSTALPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWE 243
           G   + K                L    +EE++ ++  ++++ GF GPLN+YR+ + NW+
Sbjct: 415 GFIAVHKATEIGGILVNTPEDPNLSKITTEEEIEFYIQQFKKTGFRGPLNWYRNTERNWK 474

Query: 244 LTAPWTGVQVKVPVKFIVGEVDMVYTTPGVKEYVNGGGFKKDVPFLQEVVVMEGVGHFLN 303
            +    G ++ VP   +  E D+V      K        +K +PFL+   + E  GH+  
Sbjct: 475 WSCKGLGRKILVPALMVTAEKDIVLRPEMSK------NMEKWIPFLKRGHI-EDCGHWTQ 534

Query: 304 QEKPEEIS 308
            EKP E++
Sbjct: 535 IEKPTEVN 534

BLAST of Spg028151 vs. ExPASy TrEMBL
Match: A0A5D3CIS4 (Bifunctional epoxide hydrolase 2-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004160 PE=4 SV=1)

HSP 1 Score: 606.7 bits (1563), Expect = 5.6e-170
Identity = 289/317 (91.17%), Postives = 302/317 (95.27%), Query Frame = 0

Query: 1   MDTIGHRTVSVNGINMHVAEKGEGPVVLLIHGFPELWYTWRHQILALSSLGYRAVAPDLR 60
           MDTI HRTVSVNGIN+H+AEKGEGP+VL IHGFPELWYTWRHQILALSSLGY AVAPDLR
Sbjct: 1   MDTINHRTVSVNGINVHIAEKGEGPIVLFIHGFPELWYTWRHQILALSSLGYHAVAPDLR 60

Query: 61  GYGDSDAPGSISSYSIMHIVGDLVALVESLGVKEVFVVAHDWGALIAWCLCLFRAEIVKA 120
           GYGDSDAP SISSYSIMHIVGDLVALVESLGVKEVFVVAHDWGALIAW LCLFRAEIVKA
Sbjct: 61  GYGDSDAPDSISSYSIMHIVGDLVALVESLGVKEVFVVAHDWGALIAWGLCLFRAEIVKA 120

Query: 121 FVCLSVPFRPRHPQMKPVESMRKFFGDDYYICRFQNPGEIEEEMAQVGAKEVLKGILTTR 180
           FVCLSVPFRPRHP  KPVE+MRK FGDDYYICRFQNPGEIEEEMAQVGAKEVL+GILTTR
Sbjct: 121 FVCLSVPFRPRHPNRKPVETMRKVFGDDYYICRFQNPGEIEEEMAQVGAKEVLRGILTTR 180

Query: 181 TPGPPILPKGKAFRPRPGSSTALPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL 240
             GPPI PK +AFR RP SS+ LPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL
Sbjct: 181 RQGPPIYPKKQAFRARPESSSPLPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL 240

Query: 241 TAPWTGVQVKVPVKFIVGEVDMVYTTPGVKEYVNGGGFKKDVPFLQEVVVMEGVGHFLNQ 300
           TAPWTGVQVKVPVKFIVG+VDMVYTTPGVKEYVNGGGFKKDVPFLQ+VV+MEGVGHFLNQ
Sbjct: 241 TAPWTGVQVKVPVKFIVGDVDMVYTTPGVKEYVNGGGFKKDVPFLQDVVIMEGVGHFLNQ 300

Query: 301 EKPEEISTHIHDFIKKF 318
           EKPEEI+THI+DFI+KF
Sbjct: 301 EKPEEINTHIYDFIRKF 317

BLAST of Spg028151 vs. ExPASy TrEMBL
Match: A0A1S3BNY7 (bifunctional epoxide hydrolase 2-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492144 PE=4 SV=1)

HSP 1 Score: 606.7 bits (1563), Expect = 5.6e-170
Identity = 289/317 (91.17%), Postives = 302/317 (95.27%), Query Frame = 0

Query: 1   MDTIGHRTVSVNGINMHVAEKGEGPVVLLIHGFPELWYTWRHQILALSSLGYRAVAPDLR 60
           MDTI HRTVSVNGIN+H+AEKGEGP+VL IHGFPELWYTWRHQILALSSLGY AVAPDLR
Sbjct: 1   MDTINHRTVSVNGINVHIAEKGEGPIVLFIHGFPELWYTWRHQILALSSLGYHAVAPDLR 60

Query: 61  GYGDSDAPGSISSYSIMHIVGDLVALVESLGVKEVFVVAHDWGALIAWCLCLFRAEIVKA 120
           GYGDSDAP SISSYSIMHIVGDLVALVESLGVKEVFVVAHDWGALIAW LCLFRAEIVKA
Sbjct: 61  GYGDSDAPDSISSYSIMHIVGDLVALVESLGVKEVFVVAHDWGALIAWGLCLFRAEIVKA 120

Query: 121 FVCLSVPFRPRHPQMKPVESMRKFFGDDYYICRFQNPGEIEEEMAQVGAKEVLKGILTTR 180
           FVCLSVPFRPRHP  KPVE+MRK FGDDYYICRFQNPGEIEEEMAQVGAKEVL+GILTTR
Sbjct: 121 FVCLSVPFRPRHPNRKPVETMRKVFGDDYYICRFQNPGEIEEEMAQVGAKEVLRGILTTR 180

Query: 181 TPGPPILPKGKAFRPRPGSSTALPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL 240
             GPPI PK +AFR RP SS+ LPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL
Sbjct: 181 RQGPPIYPKKQAFRARPESSSPLPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL 240

Query: 241 TAPWTGVQVKVPVKFIVGEVDMVYTTPGVKEYVNGGGFKKDVPFLQEVVVMEGVGHFLNQ 300
           TAPWTGVQVKVPVKFIVG+VDMVYTTPGVKEYVNGGGFKKDVPFLQ+VV+MEGVGHFLNQ
Sbjct: 241 TAPWTGVQVKVPVKFIVGDVDMVYTTPGVKEYVNGGGFKKDVPFLQDVVIMEGVGHFLNQ 300

Query: 301 EKPEEISTHIHDFIKKF 318
           EKPEEI+THI+DFI+KF
Sbjct: 301 EKPEEINTHIYDFIRKF 317

BLAST of Spg028151 vs. ExPASy TrEMBL
Match: A0A6J1HYW5 (uncharacterized protein LOC111468917 OS=Cucurbita maxima OX=3661 GN=LOC111468917 PE=4 SV=1)

HSP 1 Score: 603.2 bits (1554), Expect = 6.2e-169
Identity = 284/317 (89.59%), Postives = 303/317 (95.58%), Query Frame = 0

Query: 1   MDTIGHRTVSVNGINMHVAEKGEGPVVLLIHGFPELWYTWRHQILALSSLGYRAVAPDLR 60
           MD+I HRTVSVNGINMH+AEKGEGP+VLLIHGFPELWY+WRHQILALSS GYRAVAPDLR
Sbjct: 1   MDSIQHRTVSVNGINMHIAEKGEGPIVLLIHGFPELWYSWRHQILALSSHGYRAVAPDLR 60

Query: 61  GYGDSDAPGSISSYSIMHIVGDLVALVESLGVKEVFVVAHDWGALIAWCLCLFRAEIVKA 120
           GYGDSDAP S+SSYSIMHIVGDLVALVE LGVKEVFVVAHDWGA+IAWCLCLFRA+IVKA
Sbjct: 61  GYGDSDAPDSLSSYSIMHIVGDLVALVEILGVKEVFVVAHDWGAMIAWCLCLFRADIVKA 120

Query: 121 FVCLSVPFRPRHPQMKPVESMRKFFGDDYYICRFQNPGEIEEEMAQVGAKEVLKGILTTR 180
           FVCLSVPFRPRHP++KPVESMRKFFGDDYYICRFQNPGEIEEEMA VGAKEV++GILTTR
Sbjct: 121 FVCLSVPFRPRHPKVKPVESMRKFFGDDYYICRFQNPGEIEEEMAMVGAKEVVRGILTTR 180

Query: 181 TPGPPILPKGKAFRPRPGSSTALPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL 240
             GPPI PKG+AF+ RP SST LPSWLSEEDLSYFASK+E KGFTGPLNYYRSMDLNWEL
Sbjct: 181 RTGPPIFPKGQAFKSRPESSTPLPSWLSEEDLSYFASKFESKGFTGPLNYYRSMDLNWEL 240

Query: 241 TAPWTGVQVKVPVKFIVGEVDMVYTTPGVKEYVNGGGFKKDVPFLQEVVVMEGVGHFLNQ 300
           TAPWTGVQVKVPVKF+VG+VDMVYTTPGVKEYVNGGGFKKDVP+LQEVVVMEGVGHFL Q
Sbjct: 241 TAPWTGVQVKVPVKFVVGDVDMVYTTPGVKEYVNGGGFKKDVPYLQEVVVMEGVGHFLTQ 300

Query: 301 EKPEEISTHIHDFIKKF 318
           EKPEE+S+HI DFIKKF
Sbjct: 301 EKPEEVSSHILDFIKKF 317

BLAST of Spg028151 vs. ExPASy TrEMBL
Match: A0A6J1EUF9 (uncharacterized protein LOC111437874 OS=Cucurbita moschata OX=3662 GN=LOC111437874 PE=4 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 1.8e-168
Identity = 283/317 (89.27%), Postives = 302/317 (95.27%), Query Frame = 0

Query: 1   MDTIGHRTVSVNGINMHVAEKGEGPVVLLIHGFPELWYTWRHQILALSSLGYRAVAPDLR 60
           MD+I HRTVSVNGINMH+AEKGEGP+VLLIHGFPELWY+WRHQILALSS GYRAVAPDLR
Sbjct: 1   MDSIQHRTVSVNGINMHIAEKGEGPIVLLIHGFPELWYSWRHQILALSSQGYRAVAPDLR 60

Query: 61  GYGDSDAPGSISSYSIMHIVGDLVALVESLGVKEVFVVAHDWGALIAWCLCLFRAEIVKA 120
           GYGDSDAP S+SSYSIMHIVGDLVALVE LGVKEVFVVAHDWGA+I WCLCLFR +IVKA
Sbjct: 61  GYGDSDAPDSLSSYSIMHIVGDLVALVEILGVKEVFVVAHDWGAMIVWCLCLFRPDIVKA 120

Query: 121 FVCLSVPFRPRHPQMKPVESMRKFFGDDYYICRFQNPGEIEEEMAQVGAKEVLKGILTTR 180
           FVCLSVPFRPRHP++KPVESMRKFFGDDYYICRFQNPGEIEEEMA VGAKEV++GILTTR
Sbjct: 121 FVCLSVPFRPRHPKIKPVESMRKFFGDDYYICRFQNPGEIEEEMAVVGAKEVVRGILTTR 180

Query: 181 TPGPPILPKGKAFRPRPGSSTALPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL 240
             GPPI PKG+AF+ RPGSST LPSWLSEEDLSYFASK+E KGFTGPLNYYRSMDLNWEL
Sbjct: 181 KTGPPIFPKGQAFQVRPGSSTPLPSWLSEEDLSYFASKFESKGFTGPLNYYRSMDLNWEL 240

Query: 241 TAPWTGVQVKVPVKFIVGEVDMVYTTPGVKEYVNGGGFKKDVPFLQEVVVMEGVGHFLNQ 300
           TAPWTGVQVKVPVKF+VG+VDMVYTTPGVKEYVNGGGFKKDVP+LQEVVVMEGVGHFL Q
Sbjct: 241 TAPWTGVQVKVPVKFVVGDVDMVYTTPGVKEYVNGGGFKKDVPYLQEVVVMEGVGHFLTQ 300

Query: 301 EKPEEISTHIHDFIKKF 318
           EKPEE+S+HI DFIKKF
Sbjct: 301 EKPEEVSSHILDFIKKF 317

BLAST of Spg028151 vs. ExPASy TrEMBL
Match: A0A0A0LYZ6 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G569240 PE=4 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 9.0e-168
Identity = 285/317 (89.91%), Postives = 298/317 (94.01%), Query Frame = 0

Query: 1   MDTIGHRTVSVNGINMHVAEKGEGPVVLLIHGFPELWYTWRHQILALSSLGYRAVAPDLR 60
           MDTI HRTVSVNGINMH+AEKGEGP+VL IHGFPELWYTWRHQILALSSLGY AVAPDLR
Sbjct: 1   MDTISHRTVSVNGINMHIAEKGEGPIVLFIHGFPELWYTWRHQILALSSLGYHAVAPDLR 60

Query: 61  GYGDSDAPGSISSYSIMHIVGDLVALVESLGVKEVFVVAHDWGALIAWCLCLFRAEIVKA 120
           GYGDSDAP SISSYSIMHIVGDLVALVES GVKEVFVVAHDWGALIAW LCLFRAE+VKA
Sbjct: 61  GYGDSDAPDSISSYSIMHIVGDLVALVESFGVKEVFVVAHDWGALIAWSLCLFRAEMVKA 120

Query: 121 FVCLSVPFRPRHPQMKPVESMRKFFGDDYYICRFQNPGEIEEEMAQVGAKEVLKGILTTR 180
           FVCLSVPFRPRHP  KPVE+MR  FGDDYYICRFQNPGEIEEEMAQVGAK+VL+GILTTR
Sbjct: 121 FVCLSVPFRPRHPNRKPVETMRMVFGDDYYICRFQNPGEIEEEMAQVGAKDVLRGILTTR 180

Query: 181 TPGPPILPKGKAFRPRPGSSTALPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL 240
             GPPI PK +AFR R G  + LPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL
Sbjct: 181 RQGPPIYPKKQAFRARAGPPSPLPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL 240

Query: 241 TAPWTGVQVKVPVKFIVGEVDMVYTTPGVKEYVNGGGFKKDVPFLQEVVVMEGVGHFLNQ 300
           TAPWTGVQVKVPVKFIVG+VDMVYTTPGVKEYVNGGGFKKDVPFLQ+VVVM+GVGHFLNQ
Sbjct: 241 TAPWTGVQVKVPVKFIVGDVDMVYTTPGVKEYVNGGGFKKDVPFLQDVVVMDGVGHFLNQ 300

Query: 301 EKPEEISTHIHDFIKKF 318
           EKPEEI+THI+DFIKKF
Sbjct: 301 EKPEEINTHIYDFIKKF 317

BLAST of Spg028151 vs. TAIR 10
Match: AT4G02340.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 444.5 bits (1142), Expect = 7.1e-125
Identity = 197/317 (62.15%), Postives = 252/317 (79.50%), Query Frame = 0

Query: 1   MDTIGHRTVSVNGINMHVAEKGEGPVVLLIHGFPELWYTWRHQILALSSLGYRAVAPDLR 60
           M+ I H T+S NGINMHVA  G GPV+L +HGFP+LWY+WRHQ+++ ++LGYRA+APDLR
Sbjct: 1   MEKIEHTTISTNGINMHVASIGSGPVILFVHGFPDLWYSWRHQLVSFAALGYRAIAPDLR 60

Query: 61  GYGDSDAPGSISSYSIMHIVGDLVALVESLGVKEVFVVAHDWGALIAWCLCLFRAEIVKA 120
           GYGDSDAP S  SY+I+HIVGDLV L++SLGV  VF+V HDWGA++AW LC+ R + V A
Sbjct: 61  GYGDSDAPPSRESYTILHIVGDLVGLLDSLGVDRVFLVGHDWGAIVAWWLCMIRPDRVNA 120

Query: 121 FVCLSVPFRPRHPQMKPVESMRKFFGDDYYICRFQNPGEIEEEMAQVGAKEVLKGILTTR 180
            V  SV F PR+P +KPV++ R  FGDDYYICRFQ PGEIEE+ AQV  K+++    T+R
Sbjct: 121 LVNTSVVFNPRNPSVKPVDAFRALFGDDYYICRFQEPGEIEEDFAQVDTKKLITRFFTSR 180

Query: 181 TPGPPILPKGKAFRPRPGSSTALPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL 240
            P PP +PK   FR  P    +LP+WL+E+D+ ++  K+ QKGFTG LNYYR+++L+WEL
Sbjct: 181 NPRPPCIPKSVGFRGLP-DPPSLPAWLTEQDVRFYGDKFSQKGFTGGLNYYRALNLSWEL 240

Query: 241 TAPWTGVQVKVPVKFIVGEVDMVYTTPGVKEYVNGGGFKKDVPFLQEVVVMEGVGHFLNQ 300
           TAPWTG+Q+KVPVKFIVG++D+ Y  PG KEY++ GG KK VPFLQEVVVMEGVGHFL+Q
Sbjct: 241 TAPWTGLQIKVPVKFIVGDLDITYNIPGTKEYIHEGGLKKHVPFLQEVVVMEGVGHFLHQ 300

Query: 301 EKPEEISTHIHDFIKKF 318
           EKP+E++ HI+ F KKF
Sbjct: 301 EKPDEVTDHIYGFFKKF 316

BLAST of Spg028151 vs. TAIR 10
Match: AT3G05600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 441.8 bits (1135), Expect = 4.6e-124
Identity = 204/323 (63.16%), Postives = 256/323 (79.26%), Query Frame = 0

Query: 1   MDTIGHRTVSVNGINMHVAEKG--EGPVVLLIHGFPELWYTWRHQILALSSLGYRAVAPD 60
           M+ I HR VSVNGI MH+AEKG  EGPVVLL+HGFP+LWYTWRHQI  LSSLGYRAVAPD
Sbjct: 1   MEGIDHRMVSVNGITMHIAEKGPKEGPVVLLLHGFPDLWYTWRHQISGLSSLGYRAVAPD 60

Query: 61  LRGYGDSDAPGSISSYSIMHIVGDLVALVESL--GVKEVFVVAHDWGALIAWCLCLFRAE 120
           LRGYGDSD+P S S Y+ +++VGDLVAL++S+    ++VF+V HDWGA+I W LCLFR E
Sbjct: 61  LRGYGDSDSPESFSEYTCLNVVGDLVALLDSVAGNQEKVFLVGHDWGAIIGWFLCLFRPE 120

Query: 121 IVKAFVCLSVPFRPRHPQMKPVESMRKFFGDDYYICRFQNPGEIEEEMAQVGAKEVLKGI 180
            +  FVCLSVP+R R+P++KPV+  +  FGDDYYICRFQ PG+IE E+A    +  L+ +
Sbjct: 121 KINGFVCLSVPYRSRNPKVKPVQGFKAVFGDDYYICRFQEPGKIEGEIASADPRIFLRNL 180

Query: 181 LTTRTPGPPILPKGKAF--RPRPGS-STALPSWLSEEDLSYFASKYEQKGFTGPLNYYRS 240
            T RT GPPILPK   F  +P P S +  LP W S++DL ++ SK+E+ GFTG LNYYR+
Sbjct: 181 FTGRTLGPPILPKDNPFGEKPNPNSENIELPEWFSKKDLDFYVSKFEKAGFTGGLNYYRA 240

Query: 241 MDLNWELTAPWTGVQVKVPVKFIVGEVDMVYTTPGVKEYVNGGGFKKDVPFLQEVVVMEG 300
           MDLNWELTAPWTG +++VPVKF+ G+ DMVYTTPG+KEY++GGGF  DVP LQE+VV+E 
Sbjct: 241 MDLNWELTAPWTGAKIQVPVKFMTGDFDMVYTTPGMKEYIHGGGFAADVPTLQEIVVIED 300

Query: 301 VGHFLNQEKPEEISTHIHDFIKK 317
            GHF+NQEKP+E++ HI+DF  K
Sbjct: 301 AGHFVNQEKPQEVTAHINDFFTK 323

BLAST of Spg028151 vs. TAIR 10
Match: AT4G15960.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 424.9 bits (1091), Expect = 5.8e-119
Identity = 190/324 (58.64%), Postives = 250/324 (77.16%), Query Frame = 0

Query: 1   MDTIGHRTVSVNGINMHVAEK-----GEGPVVLLIHGFPELWYTWRHQILALSSLGYRAV 60
           +D + H+T+ VNGINMHVAEK     GE P++L +HGFPELWYTWRHQ++ALSSLGYR +
Sbjct: 51  LDGVEHKTLKVNGINMHVAEKPGSGSGEDPIILFLHGFPELWYTWRHQMVALSSLGYRTI 110

Query: 61  APDLRGYGDSDAPGSISSYSIMHIVGDLVALVESL--GVKEVFVVAHDWGALIAWCLCLF 120
           APDLRGYGD++AP  +  Y+ +++ GD+VAL++++  G K V VV HDWGA+IAW LC +
Sbjct: 111 APDLRGYGDTEAPEKVEDYTYLNVDGDVVALIDAVTGGDKAVSVVGHDWGAMIAWQLCQY 170

Query: 121 RAEIVKAFVCLSVPFRPRHPQMKPVESMRKFFGDDYYICRFQNPGEIEEEMAQVGAKEVL 180
           R E VKA V +SV F PR+P   PV ++R  FGDDYY+CRFQ  GEIE E  ++G + VL
Sbjct: 171 RPEKVKALVNMSVLFSPRNPVRVPVPTLRHVFGDDYYVCRFQKAGEIETEFKKLGTENVL 230

Query: 181 KGILTTRTPGPPILPKGKAFRPRPGSSTALPSWLSEEDLSYFASKYEQKGFTGPLNYYRS 240
           K  LT +TPGP  LPK K F+    +++ALP WL++EDL Y+ +KYE KGFTGP+NYYR+
Sbjct: 231 KEFLTYKTPGPLNLPKDKYFKRSENAASALPLWLTQEDLDYYVTKYENKGFTGPINYYRN 290

Query: 241 MDLNWELTAPWTGVQVKVPVKFIVGEVDMVYTTPGVKEYVNGGGFKKDVPFLQEVVVMEG 300
           +D NWELTAPWTG +++VPVKFI+G+ D+ Y  PG KEY+NGGGFK+DVP L E VV++G
Sbjct: 291 IDRNWELTAPWTGAKIRVPVKFIIGDQDLTYNFPGAKEYINGGGFKRDVPLLDETVVLKG 350

Query: 301 VGHFLNQEKPEEISTHIHDFIKKF 318
           +GHFL++E P+ I+ HIH+F  KF
Sbjct: 351 LGHFLHEENPDVINQHIHNFFHKF 374

BLAST of Spg028151 vs. TAIR 10
Match: AT2G26740.1 (soluble epoxide hydrolase )

HSP 1 Score: 417.2 bits (1071), Expect = 1.2e-116
Identity = 198/319 (62.07%), Postives = 249/319 (78.06%), Query Frame = 0

Query: 6   HRTVSVNGINMHVAEKG--EGPVVLLIHGFPELWYTWRHQILALSSLGYRAVAPDLRGYG 65
           HR V  NGI++HVA +G  +GP+VLL+HGFPELWY+WRHQI  L++ GYRAVAPDLRGYG
Sbjct: 3   HRKVRGNGIDIHVAIQGPSDGPIVLLLHGFPELWYSWRHQIPGLAARGYRAVAPDLRGYG 62

Query: 66  DSDAPGSISSYSIMHIVGDLVALVESLGVKE---VFVVAHDWGALIAWCLCLFRAEIVKA 125
           DSDAP  ISSY+  +IVGDL+A++ +L   E   VFVV HDWGALIAW LCLFR + VKA
Sbjct: 63  DSDAPAEISSYTCFNIVGDLIAVISALTASEDEKVFVVGHDWGALIAWYLCLFRPDRVKA 122

Query: 126 FVCLSVP--FRPRHPQMKPVESMRKFFGDDYYICRFQNPGEIEEEMAQVGAKEVLKGILT 185
            V LSVP  FRP  P +KPV+ MR F+GDDYYICRFQ  G++E E+A+VG + V+K +LT
Sbjct: 123 LVNLSVPFSFRPTDPSVKPVDRMRAFYGDDYYICRFQEFGDVEAEIAEVGTERVMKRLLT 182

Query: 186 TRTPGPPILPKGKAFRPRPGSSTALPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNW 245
            RTPGP I+PK K+F    G +  LPSWL+EED++YF SK+E+KGF+GP+NYYR+ + N 
Sbjct: 183 YRTPGPVIIPKDKSFWGSKGETIPLPSWLTEEDVAYFVSKFEEKGFSGPVNYYRNFNRNN 242

Query: 246 ELTAPWTGVQVKVPVKFIVGEVDMVYTTPGVKEYVNGGGFKKDVPFLQEVVVMEGVGHFL 305
           EL  PW G +++VP KF++GE+D+VY  PGVKEY++G  FK+DVP L+E VVMEGV HF+
Sbjct: 243 ELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLLEEPVVMEGVAHFI 302

Query: 306 NQEKPEEISTHIHDFIKKF 318
           NQEKP+EI   I DFI KF
Sbjct: 303 NQEKPQEILQIILDFISKF 321

BLAST of Spg028151 vs. TAIR 10
Match: AT2G26750.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 401.4 bits (1030), Expect = 6.9e-112
Identity = 192/318 (60.38%), Postives = 245/318 (77.04%), Query Frame = 0

Query: 6   HRTVSVNGINMHVAEKG--EGPVVLLIHGFPELWYTWRHQILALSSLGYRAVAPDLRGYG 65
           HR V  NGI++HVA +G  +G +VLL+HGFPELWY+WRHQI  L++ GYRAVAPDLRGYG
Sbjct: 3   HRNVRGNGIDIHVAIQGPSDGTIVLLLHGFPELWYSWRHQISGLAARGYRAVAPDLRGYG 62

Query: 66  DSDAPGSISSYSIMHIVGDLVALVESL--GVKEVFVVAHDWGALIAWCLCLFRAEIVKAF 125
           DSDAP  ISS++  +IVGDLVA++ +L    K+VFVV HDWGALIAW LCLFR + VKA 
Sbjct: 63  DSDAPAEISSFTCFNIVGDLVAVISTLIKEDKKVFVVGHDWGALIAWYLCLFRPDKVKAL 122

Query: 126 VCLSVP--FRPRHPQMKPVESMRKFFGDDYYICRFQNPGEIEEEMAQVGAKEVLKGILTT 185
           V LSVP  F P  P +KPV+ MR  +G+DYY+CRFQ  G+IE E+A+VG + V+K +LT 
Sbjct: 123 VNLSVPLSFWPTDPSVKPVDRMRAVYGNDYYVCRFQEVGDIEAEIAEVGTERVMKRLLTY 182

Query: 186 RTPGPPILPKGKAFRPRPGSSTALPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWE 245
           RTPGP I+PK K+F    G +  LPSWL+EED++YF SK+++KGF GP+NYYR+ + N E
Sbjct: 183 RTPGPLIIPKDKSFWGSKGETIPLPSWLTEEDVAYFVSKFKEKGFCGPVNYYRNFNRNNE 242

Query: 246 LTAPWTGVQVKVPVKFIVGEVDMVYTTPGVKEYVNGGGFKKDVPFLQEVVVMEGVGHFLN 305
           L  PW G +++VP KF++GE+D+VY  PGVKEY++G  FK+DVP ++E VVMEGV HFLN
Sbjct: 243 LLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLIEEPVVMEGVAHFLN 302

Query: 306 QEKPEEISTHIHDFIKKF 318
           QEKP+EI   I DFI  F
Sbjct: 303 QEKPQEILQIILDFISTF 320

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038879653.11.2e-17794.64epoxide hydrolase A-like [Benincasa hispida][more]
XP_008450607.11.2e-16991.17PREDICTED: bifunctional epoxide hydrolase 2-like isoform X1 [Cucumis melo] >KAA0... [more]
XP_023531428.11.3e-16889.59uncharacterized protein LOC111793669 [Cucurbita pepo subsp. pepo][more]
XP_022969836.11.3e-16889.59uncharacterized protein LOC111468917 [Cucurbita maxima][more]
KAG6588564.13.7e-16889.59hypothetical protein SDJN03_17129, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
I6YGS04.3e-5839.06Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX... [more]
P349136.4e-4632.55Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2[more]
Q6Q2C24.2e-4531.70Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1[more]
P802993.5e-4431.35Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=... [more]
P349141.5e-4230.52Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A5D3CIS45.6e-17091.17Bifunctional epoxide hydrolase 2-like isoform X1 OS=Cucumis melo var. makuwa OX=... [more]
A0A1S3BNY75.6e-17091.17bifunctional epoxide hydrolase 2-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A6J1HYW56.2e-16989.59uncharacterized protein LOC111468917 OS=Cucurbita maxima OX=3661 GN=LOC111468917... [more]
A0A6J1EUF91.8e-16889.27uncharacterized protein LOC111437874 OS=Cucurbita moschata OX=3662 GN=LOC1114378... [more]
A0A0A0LYZ69.0e-16889.91AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G569... [more]
Match NameE-valueIdentityDescription
AT4G02340.17.1e-12562.15alpha/beta-Hydrolases superfamily protein [more]
AT3G05600.14.6e-12463.16alpha/beta-Hydrolases superfamily protein [more]
AT4G15960.15.8e-11958.64alpha/beta-Hydrolases superfamily protein [more]
AT2G26740.11.2e-11662.07soluble epoxide hydrolase [more]
AT2G26750.16.9e-11260.38alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000639Epoxide hydrolase-likePRINTSPR00412EPOXHYDRLASEcoord: 51..66
score: 63.92
coord: 111..124
score: 38.96
coord: 251..267
score: 40.91
coord: 291..313
score: 45.45
coord: 97..110
score: 55.52
coord: 31..49
score: 55.98
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 1..317
e-value: 6.5E-108
score: 363.2
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 3..316
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 25..166
e-value: 1.4E-21
score: 77.4
NoneNo IPR availablePANTHERPTHR43329:SF58OS05G0273800 PROTEINcoord: 1..317
NoneNo IPR availablePANTHERPTHR43329EPOXIDE HYDROLASEcoord: 1..317

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg028151.1Spg028151.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0004301 epoxide hydrolase activity
molecular_function GO:0003824 catalytic activity