Spg028141 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg028141
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptiontRNA-specific 2-thiouridylase MnmA
Locationscaffold2: 44669204 .. 44676081 (+)
RNA-Seq ExpressionSpg028141
SyntenySpg028141
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTCGGTTCAGTTTTCGGTTTGTCTTTGTCCGATCCGAACCAAGATGCGCTTGCTTATGCAGACCTTGAATCGATCTCTCAGCCGTCTCTCTGGTCTCTCAAACCGATCTTACAGCTGAATCAGTTTGCGCCATTGCTCGACGACGTGCTACTGCTCCTCGCCGGCGCCGTTTAATCTCCCTCTCTCTCTGCTCGTGGTCGTGCTCTTACATCTGTGTTTAATCTCCCTCTCTCTCTCTGGTCTCTCTGCTCGTCGCCGGTGCCTAAAAGTGGTCAGTCCGTCAAACCAAACCTCTCTTCTTTTCTGCTCTGTTTTATGCGTTTTATATGCTTTAATTACTGAACCGATTTGTGATTAACTGCTGCAAAGTTCATTGAGTCTGTTGCCCTGAATGTGTTCATTGAGTCTTCTGCAAAGTTCTTTTATGCTCTGTTTTATTTGCTTTGTTAAACCTGTTTGATAAAATGCCTCTGCGAATGTGTTGTGTTTTTTTGTCAATGCTGAAGTAGTTGATGATCAGGGGTTCTTTGATTATGGTTTGAGGAGATGTTATGGGGTTCTATTGAATTGGATTGAGTTGGTGTCCTGCCTGAATAAAGTCTTCTTTAATATCTTTTATGAATATAATGACAGAAACTAGCCCTCACTTTCACGAAAGGATGAAGTAATCAATTGGAATTGTAAACTACTTGCTTTTACTATTTAGAATAATAGAGGCTAGCTTAAATAATTTAGCATAAGATTTGTTAAATCTTGTTGAAAATATCTGATTCCTGAGAATGTTCTTTATTTCCTTATCCTTCTAGCCCTCACTTGCACGAAAGGATGAAGTAATCAATTGGAATTATAAACTACTTGTCATCACTATTTAAAATGATAGAGGCTAGCTTAAATAATTTAGGCTAGCATAAGATTTGTTAAATCTTGTTGAAAATATCTGATTCCTTAGCAAGTTCTTTATTTCCTTATCCTTCCTTATGGAAAAAAATGTCATTCATCCAATTTATGAAGATATGTCTTGTGTAAAGTATCTTGTGGTAGAATTCATGTTTCTTTAATGTGTAGATAAAATTTAGTGAATTTGTTGCAAGACATATTGGGGAAGAGGAATGTGTCATATTGGAGGCGGAGTTGGGGAATTTTCTTGGCAAACATCGAGGATTTTGGTTCTACGTGATTGGCCAATGCCAAGGTCTAAGACTTCCTGGAGGACCTTGGTATGCCTTTTCAGTTCCGTTTCATCAAGGCCATTACCAAATCTTCAAGAACATCTTAAATTCTAATCGGTAGATTAGATTTGTACATTCCAGGGTGCTAGTTTAGCTTTTAATAACTAATTACGTTGGATTATTTCCAATCCGGTGCTTCACAAGTTCACATTCTTTAGTTACATACTTACATCTTTTGCTGAACTAATTTACAATGACGATTTCAGCTTATATGCTTTGGTCTAATGCAGGTATGTTGTGGGAAAAGATAGAAAGAACAATGTAGTATTTGTGTCGAGAAATTACGTTGGAATTGTTGTCTTGTTTAACAATAAAGCTTTATCTATGTTGCTTTGCTTTTAACAATAGGGATTTGAGAAATTTATACAAAAGGGTGGTTTTATCAATTGGTTGTCAATATGCCCCCATTAATTTGTCTAGTTGTTGTAAAATCCCCATTTTGAGCCTATTTCCATGTCAAGTGAGTCTTTTCATTTTCAAGCACAATACGTTTTACTCCACCTCCTACTCTTCCATATTAAAAACATTAAATTTTTTGTGACATTTCCAACAGGAGCTCAGTGGTAAAAATCAACTCAAACCCATAGTTTACGCAACCTTTGGTGACCCAGTTAAGAACAAAAAACAATTGGAGGAGAGTTACCAAGTGCAACCCTCGCCGTTGGCGATCACCCTCTTGTTCTTAGGTGAACACTTTGCACTTTTCTTCTTTCTCTGTGAAAAGCATTTCAATGGTAGCTTATGAATGGTTGTACAAGATCGTTTCGGCTTGTTCTTAGAGCTTCAATGCAAGTTCTAGTATCATCTCTCTCAAAATTTCTTGTGTGGTTTGTAAATGGTTGTTTCTTTTTCTCTAATCGATTTTTGGATTCTCTGAAGTTCAAAATGCAAAGATCGTTCTGTTTAGCCGGTCAACTGGTGAAGAGGCGAGGATTTTCGACGAATTCCGATAAAATTGTGGCATCTGTACTGTTTGAAAGGCTGCCAGTCGTGATTCCGAAGATTGATCCTGTTGTCTATGCATTTCAGGAGTTTAAGTAAGTCTATTTTCTTGTATTTCTATGTTAATTTTTGAGAACCGAGAAATGGTGAAAATGGAAAAATAAAATGAAGGAAACTAGGAGTATATCTTGACTTGATATTCATAGAGAACACGGATTAATCAGGAACTGGAATTCGAACAACGTGATTAGTGTGATAGAGTTATGAGTTCGCACTAAAATCAAGTAACATTGTTATGGCATACTCTCCACAGAATCATTTTTCTAATACTTCTGCTTGATTGTGCTCAGATTTAGGTGGCAACAACAATATCGACGCAAATATCCTGATAAATTTTTAGATAAAGCTAATGCCAGGTAAATGATCTATCATTTTGTTAGGCTGTGTCCTGTACGTGATTTTGGTTTATGAATTCTTTTAATTTCTCTTTTTTCAACTCTTTTAATTCTTGCGCTATTTTTTTTTTCTTAATTGGAAACAAATTTTATTGATGTATGAAATTTACAAAAGAAGGACGAAGCCCAAACTAAAGGAGTTACAAAAGACTTCTCCAATTGGTCAAAAGGGTGGAAAGATTGTAAGAGCTAAAGAAGGGTGAGCATTTACACCAAGACATAGCAAGGAAAACAATCTTTTAAAGAAAGCTATCGAAACGGCTTTCTTTCTCACTGAAGAGACAGTTGTTACGTTCCATCCAAGTCGATCACAAAAAAGCCCGAATAAGATGCATCCAAAGAACTTTCTTTCCATCAGAAAAAGGATGCCCATTAAGCGTATAGATAAGAAGGCTGTTGAGCTCTCTAGGTAAAGTGGAGTGCCAATTGAAGGTTGATGAAATCTCCTCCCAAAGTCTGTTGGCGTATTTGCAAGAAATGAATAAGTGGCTTTGAGTTTCCTGAGCTGTTTTACAGATTGTACAGCAGTGAGGAGATAAAGCCATGTGAGGTAAATGGCGTTGCAGTTTGTCATATGTGTTTATGGCATTGTGGCTAAGCTCCCACAAGAAAAACCTAATTTTCTTAGGGTAAAGTCTAAAGATCTGACCATATTGCTTTGTGAAGGGGGGGGGGGGGGGGGGGGGGGGGGGTATTGTTGCTGTCATGTTATTGTCTAGGTCAAGCGAGATTTTGTGGAGAAAGAGCCCTTTGGGTCCAGTTTCCATATCCATTGATCCTTGGATAGAGTAAGCTGAACACTATTCAAGAGATCTAATAGTCTAACCCATTCTTCAATTTCATCTTCCATGAGGTTTCTCCTCAATTTTAAATTCCACCCACCATGTTGGTTGTTCCATGAGCCTTGTATGGAGATATCTTTGTACAGAGACAAAGAGTATAACCTGGGAAATTGATTGGCCAAGGCGTCTTCATTCAACCAGCATGTAAGAGTAGTAAGAAGCACGAACACTTCTAAAATGACGAAGTGTCAGTGTCCGACACCGACACGCCTCCGACACGCCACGTGGCATGTCTGATTTTTTTTATTTTTTATTTTTTATTTTCCGACATGGCTTCGACACGGCTCCCACAAGATTTTTTTTTTCTTTTTTTTTTTTTTCTGTTTTTATCCATAGTTATGTTTTTATCCTTGTAGTTTTTTTTTTCTGTTTTTATCCTTCACTGAAGCCTTTGGGTTTTTTTTTTTTCTGTTTTTAACCATAGTTCTGTTTTTTTTTCTCTTTTTCTTTTTTTGTTTTTATCCATAGTTCTGTTTTTTTTCTTTTTTTTTTTTCTTTTTCTGTTTTTATTCATAGTTTTGTTCTTTTTCTGTTTTTATCCATAGTTCTGTTTTTTACTTGTTTTAATGTTTAAATATAGAGGAAATTTACATAAAAGTATTAAATATTCATAATATGTCAAAATATGCACACATATATATGTTTTTTTTTTTTTTAAAATTAACGTGTCCCCAACATGTCGTGTCCTACTTTTTTGGAAATTGACGTGTCGCCGTGTCCGTGTCGTGTCGTGTCCGTGTCGCGTGTCAGTGTCTGTGCTTCTTAGAAGAGTACTAATAGGTAGGGGTGTTCATTGGTCGGTCGGTTTTAGTTCTTGTCGATTTTCTAAAGCTTGGTGATGCCGATCGACTGAACTCACGGTCGGTCGGTTGGTTTTTGTCGGTTTTGTAACTCCGGCAAAATAAATAAAAAAGAAAGAAAGGATATGGAAAGAAAGAAAAAAAAAGGAGAAACAGAAAGAAGAAAATAAAAAATGGGAAGGGAGAAGAAATCCAATGATGGAGATGGAGAAGAGAAAGAACGAGGGAGAAGGAATAAGAGAAAAAAGAGATGGAGAAGAGAAACGAACTAAATTAAAAAACATAAGAAAATAAGAAAAAAAAAGAAAAAGAGATGGAGAAGACAATGATGGCAGACCGACGGTGGCTGTGGACAGTGGGAGGTGGAAAGTTTGAGAGAAGAGAAAGAACAAGGGACTCCCATATAGAAGTAACTCAACATTTGCAGCTTAACATCACCAACAGAAAGAAGAGAACAAGTGAACAACGGAGAAAGAATGGAGAGAGGGGAAAGAAGAGAGAAGAAGATGATGAGATTGGGAAAAGAAAATGTGGGAGGAAGTGAGAGGAGAGAGAAAAAGATAAAAAAAACAATTTTTTTTTAAAATCCAATGCCATCAGAGAATAAATAAGGCAAATATGTTTTTTTCCTTCCCCTCTTATATAATCGGTCAATCGGTCGAAATTTTCTACTTTTTTTCTCCGACCGACCGACTAGTTGGTTTTATGAAATAAAAAACCGACTCCGACCTATATACCCCTTAAACCGACCGACCGACGTCAGTTTGGTCGGTTTTTGGGGTTATTTTGAACACCCCTACTAATGGGTATGCGACGAACGGTGAGGTGATTTGTGTTTGGTGGTCGATGAAAATAGGGAGGAGTGCGATTGGGAGGGAGTGGGCTGCCCGCTGCTGTGTTGTGCGGCTGGAATCGAGAAGGAAGGGAGATACTGAGATTTAGAGGTGAGATGAAATAAGATTAGGGTTTTACTTTTGGGTATATATATATATATATATATATATTGGTTCGGTTCGGTTTTTTACTGGTTTTTTCAATTAAGAACCGAACCGAACCGAAAAAATTCGGTTTTCTCTAAAATCAAACCGAGATGTCCAATAATTTGCTAAAAAACCGAACCAAACCAGTGGATCGGTTCGGTTTGGTTCGGTTTTTCGGTTTTTCAGTTTTAGCTTGCACTCCTAGTTGAGAGTATTAGTAGAATATTAGTCAATTTTGTTCGAGTATTTGTTAGGTACCTGAACATAGAAAACTCACGATAGTAATATATTGCAATATTAAAAGAAAATTAGAATATCAATAGCCTTTTGAGAGGGCTGTCTCTCCCTCAAGAGTCCCAGAATGGACATTACCTTTAAAATTCTTCACCTCCTTCTAAGATCCCTAACTTCCATATTTATAACCAAGACATTCCTAGCTAGTTTCCATTATACCCTCACTAATATTCTACTAAATACTCTCACCATAATCCGTATTAGTACTCTCACACAACAATCTTGCCAAAAGGATGTATTGACTCCATTCCCCATCTTCTATTTTACTCTCTCACGAATGAGATTTAAATGTTTCGTAATAGATCTCCATGGCCCCCTAAAATATGCTAGAAAACTGTAATTCGACCATGATTCCAATGGAAATTTTGCATATCCAGCATCAATCACTTGTCACCATAAAGCATCTCCTTTATGATAATATCTCCAAATCCATTTGGCTAATAACGCTTGTTCTTTTCTACTATTCCTTGTAATCCAAGGCCTCCATGATGTGTGATGTTGCATAAATTGCTCACCCAAGTGTACGGGTCAAGTTTTAATATAGTGATGAAAGAGAGTGTCGTCCTCAGGATTGGTTAGAAGTTCACTTAATTACCACATATATCTTTGCCCAATTTTATTTAGAGAAATCAATTGGATCAAGAGAAAAAGAATCTAAAATTAACAAAAACAAATTATTCTAGGGAAAACTCAATGGAGAAAGGTCTTGGGGAATTGACTTCACAAATTAGACATATGAATTAATTCATTAATTAACCAAGATTGAGCATGCAATTATGCTATAAACTAAGAATTCACAATTATCGACTGTTTCTCCCAAGCACAATTAATTCTTTATGCTAAATCAAGATATCGATATTCCTATCTAACTTCAATAACAAGCATAAACATTAAATCTAATTATTTTAACGATTTCTCAAGCAATCCTAAATCTCCTGATTTTAGAAAAACTTAAGTCATGTAACAATGGCCCCCTGGGGCCTAGGCGCAAGGCACACAAAAAGGCGTGGGCTTTTTTTTTTAAGGCGCACTATATAGAAAAAAAAAACTTACAAAAATATATACATGTATATAAAACACTTTCATGGCAAGTAATGGAGTTTTTAATCAAGAAAAATAGATAAAAGATAAACAAGGTTCAAGTATTAGTATTCCAACATAGAGTCAAATGTCAATACTCCACAAAAAACCGTGAGTTTAGTATTGTTGATATGCCTTGAATCTGATATCTGAAGCCCCAGAAGCGCCCAAGGCGCTCAAGGGCTTCGAGGAAGATGCGCGCCTTGGCCGCGCCTTCCCCACCGCGCCTCGCTTTTTCGAAGCGAGGCGCTAA

mRNA sequence

ATGTTCGGTTCAGTTTTCGGTTTGTCTTTGTCCGATCCGAACCAAGATGCGCTTGCTTATGCAGACCTTGAATCGATCTCTCAGCCGTCTCTCTGGTCTCTCAAACCGATCTTACAGCTGAATCAGTTTGCGCCATTGCTCGACGACATAAAATTTAGTGAATTTGTTGCAAGACATATTGGGGAAGAGGAATGTGTCATATTGGAGGCGGAGTTGGGGAATTTTCTTGGCAAACATCGAGGATTTTGGTTCTACGTGATTGGCCAATGCCAAGGTCTAAGACTTCCTGGAGGACCTTGGTATGTTGTGGGAAAAGATAGAAAGAACAATTTCAAAATGCAAAGATCGTTCTGTTTAGCCGGTCAACTGGTGAAGAGGCGAGGATTTTCGACGAATTCCGATAAAATTGTGGCATCTGTACTGTTTGAAAGGCTGCCAGTCGTGATTCCGAAGATTGATCCTGTTGTCTATGCATTTCAGGAGTTTAAATTTAGGTGGCAACAACAATATCGACGCAAATATCCTGATAAATTTTTAGATAAAGCTAATGCCAGATTGTACAGCAGTGAGGAGATAAAGCCATGTGAGGGAGGAGTGCGATTGGGAGGGAGTGGGCTGCCCGCTGCTGTGTTGTGCGGCTGGAATCGAGAAGGAAGGGAGATACTGAGATTTAGAGCCCCAGAAGCGCCCAAGGCGCTCAAGGGCTTCGAGGAAGATGCGCGCCTTGGCCGCGCCTTCCCCACCGCGCCTCGCTTTTTCGAAGCGAGGCGCTAA

Coding sequence (CDS)

ATGTTCGGTTCAGTTTTCGGTTTGTCTTTGTCCGATCCGAACCAAGATGCGCTTGCTTATGCAGACCTTGAATCGATCTCTCAGCCGTCTCTCTGGTCTCTCAAACCGATCTTACAGCTGAATCAGTTTGCGCCATTGCTCGACGACATAAAATTTAGTGAATTTGTTGCAAGACATATTGGGGAAGAGGAATGTGTCATATTGGAGGCGGAGTTGGGGAATTTTCTTGGCAAACATCGAGGATTTTGGTTCTACGTGATTGGCCAATGCCAAGGTCTAAGACTTCCTGGAGGACCTTGGTATGTTGTGGGAAAAGATAGAAAGAACAATTTCAAAATGCAAAGATCGTTCTGTTTAGCCGGTCAACTGGTGAAGAGGCGAGGATTTTCGACGAATTCCGATAAAATTGTGGCATCTGTACTGTTTGAAAGGCTGCCAGTCGTGATTCCGAAGATTGATCCTGTTGTCTATGCATTTCAGGAGTTTAAATTTAGGTGGCAACAACAATATCGACGCAAATATCCTGATAAATTTTTAGATAAAGCTAATGCCAGATTGTACAGCAGTGAGGAGATAAAGCCATGTGAGGGAGGAGTGCGATTGGGAGGGAGTGGGCTGCCCGCTGCTGTGTTGTGCGGCTGGAATCGAGAAGGAAGGGAGATACTGAGATTTAGAGCCCCAGAAGCGCCCAAGGCGCTCAAGGGCTTCGAGGAAGATGCGCGCCTTGGCCGCGCCTTCCCCACCGCGCCTCGCTTTTTCGAAGCGAGGCGCTAA

Protein sequence

MFGSVFGLSLSDPNQDALAYADLESISQPSLWSLKPILQLNQFAPLLDDIKFSEFVARHIGEEECVILEAELGNFLGKHRGFWFYVIGQCQGLRLPGGPWYVVGKDRKNNFKMQRSFCLAGQLVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRRKYPDKFLDKANARLYSSEEIKPCEGGVRLGGSGLPAAVLCGWNREGREILRFRAPEAPKALKGFEEDARLGRAFPTAPRFFEARR
Homology
[Close]
BLAST of Spg028141 vs. NCBI nr
Match: XP_022992541.1 (54S ribosomal protein L17, mitochondrial-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 141.7 bits (356), Expect = 8.6e-30
Identity = 68/77 (88.31%), Postives = 73/77 (94.81%), Query Frame = 0 

Query: 109 NNFKMQRSFCLAGQLVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQ 168
           + F+MQRSF LA QL KRRGFSTNS+KIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQ
Sbjct: 41  SQFRMQRSFSLASQLAKRRGFSTNSEKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQ 100

Query: 169 QYRRKYPDKFLDKANAR 186
           QYRR+YPD+FLDKANAR
Sbjct: 101 QYRRRYPDEFLDKANAR 117

[Close]
BLAST of Spg028141 vs. NCBI nr
Match: KAG7027644.1 (hypothetical protein SDJN02_11659 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 141.0 bits (354), Expect = 1.5e-29
Identity = 68/75 (90.67%), Postives = 72/75 (96.00%), Query Frame = 0 

Query: 111 FKMQRSFCLAGQLVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQY 170
           F+MQ SF L GQLVKRRGFSTNS+KIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQY
Sbjct: 3   FRMQSSFSLTGQLVKRRGFSTNSEKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQY 62

Query: 171 RRKYPDKFLDKANAR 186
           RR+YPD+FLDKANAR
Sbjct: 63  RRRYPDEFLDKANAR 77

[Close]
BLAST of Spg028141 vs. NCBI nr
Match: KAG6595682.1 (39S ribosomal protein L46, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 141.0 bits (354), Expect = 1.5e-29
Identity = 68/75 (90.67%), Postives = 72/75 (96.00%), Query Frame = 0 

Query: 111 FKMQRSFCLAGQLVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQY 170
           F+MQ SF L GQLVKRRGFSTNS+KIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQY
Sbjct: 3   FRMQSSFSLTGQLVKRRGFSTNSEKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQY 62

Query: 171 RRKYPDKFLDKANAR 186
           RR+YPD+FLDKANAR
Sbjct: 63  RRRYPDEFLDKANAR 77

[Close]
BLAST of Spg028141 vs. NCBI nr
Match: XP_022966446.1 (39S ribosomal protein L46, mitochondrial-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 140.2 bits (352), Expect = 2.5e-29
Identity = 68/74 (91.89%), Postives = 72/74 (97.30%), Query Frame = 0 

Query: 112 KMQRSFCLAGQLVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYR 171
           +MQ SF LAGQLVKRRGFSTNS+KIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYR
Sbjct: 9   RMQSSFSLAGQLVKRRGFSTNSEKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYR 68

Query: 172 RKYPDKFLDKANAR 186
           R+YPD+FLDKANAR
Sbjct: 69  RRYPDEFLDKANAR 82

[Close]
BLAST of Spg028141 vs. NCBI nr
Match: XP_022925098.1 (39S ribosomal protein L46, mitochondrial-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 140.2 bits (352), Expect = 2.5e-29
Identity = 68/74 (91.89%), Postives = 72/74 (97.30%), Query Frame = 0 

Query: 112 KMQRSFCLAGQLVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYR 171
           +MQ SF LAGQLVKRRGFSTNS+KIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYR
Sbjct: 9   RMQSSFSLAGQLVKRRGFSTNSEKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYR 68

Query: 172 RKYPDKFLDKANAR 186
           R+YPD+FLDKANAR
Sbjct: 69  RRYPDEFLDKANAR 82

[Close]
BLAST of Spg028141 vs. ExPASy Swiss-Prot
Match: B2RHP0 (tRNA-specific 2-thiouridylase MnmA OS=Porphyromonas gingivalis (strain ATCC 33277 / DSM 20709 / CIP 103683 / JCM 12257 / NCTC 11834 / 2561) OX=431947 GN=mnmA PE=3 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 3.4e-13
Identity = 31/64 (48.44%), Postives = 47/64 (73.44%), Query Frame = 0 

Query: 47  LDDIKFSEFVARHIGEEECVILEAELGNFLGKHRGFWFYVIGQCQGLRLPGGPWYVVGKD 106
           L  I +++F+ R++G++E  I+E E G  +G+H+G+WF+ IGQ +GL L GGPW+VV KD
Sbjct: 195 LGKIDYNDFIERYLGKKEGRIIELETGKVIGRHQGYWFHTIGQRKGLGLSGGPWFVVKKD 254

Query: 107 RKNN 111
            K N
Sbjct: 255 IKRN 258

[Close]
BLAST of Spg028141 vs. ExPASy Swiss-Prot
Match: Q7MAW9 (tRNA-specific 2-thiouridylase MnmA OS=Porphyromonas gingivalis (strain ATCC BAA-308 / W83) OX=242619 GN=mnmA PE=3 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 3.4e-13
Identity = 31/64 (48.44%), Postives = 47/64 (73.44%), Query Frame = 0 

Query: 47  LDDIKFSEFVARHIGEEECVILEAELGNFLGKHRGFWFYVIGQCQGLRLPGGPWYVVGKD 106
           L  I +++F+ R++G++E  I+E E G  +G+H+G+WF+ IGQ +GL L GGPW+VV KD
Sbjct: 195 LGKIDYNDFIERYLGKKEGRIIELETGKVIGRHQGYWFHTIGQRKGLGLSGGPWFVVKKD 254

Query: 107 RKNN 111
            K N
Sbjct: 255 IKRN 258

[Close]
BLAST of Spg028141 vs. ExPASy Swiss-Prot
Match: Q5LFN5 (tRNA-specific 2-thiouridylase MnmA 2 OS=Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343) OX=272559 GN=mnmA2 PE=3 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 4.4e-13
Identity = 32/64 (50.00%), Postives = 46/64 (71.88%), Query Frame = 0 

Query: 47  LDDIKFSEFVARHIGEEECVILEAELGNFLGKHRGFWFYVIGQCQGLRLPGGPWYVVGKD 106
           L  I +++FV R +GE+E  ++E E G  +G HRG+WF+ IGQ +GL L GGPW+VV KD
Sbjct: 194 LGKINYNDFVRRFLGEKEGAVIEFETGKKIGTHRGYWFHTIGQRKGLGLGGGPWFVVKKD 253

Query: 107 RKNN 111
            ++N
Sbjct: 254 IQDN 257

[Close]
BLAST of Spg028141 vs. ExPASy Swiss-Prot
Match: Q64WG8 (tRNA-specific 2-thiouridylase MnmA 2 OS=Bacteroides fragilis (strain YCH46) OX=295405 GN=mnmA2 PE=3 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 4.4e-13
Identity = 32/64 (50.00%), Postives = 46/64 (71.88%), Query Frame = 0 

Query: 47  LDDIKFSEFVARHIGEEECVILEAELGNFLGKHRGFWFYVIGQCQGLRLPGGPWYVVGKD 106
           L  I +++FV R +GE+E  ++E E G  +G HRG+WF+ IGQ +GL L GGPW+VV KD
Sbjct: 194 LGKINYNDFVRRFLGEKEGAVIEFETGKKIGTHRGYWFHTIGQRKGLGLGGGPWFVVKKD 253

Query: 107 RKNN 111
            ++N
Sbjct: 254 IQDN 257

[Close]
BLAST of Spg028141 vs. ExPASy Swiss-Prot
Match: Q8ABF5 (tRNA-specific 2-thiouridylase MnmA 1 OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482) OX=226186 GN=mnmA1 PE=3 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 5.8e-13
Identity = 32/64 (50.00%), Postives = 44/64 (68.75%), Query Frame = 0 

Query: 47  LDDIKFSEFVARHIGEEECVILEAELGNFLGKHRGFWFYVIGQCQGLRLPGGPWYVVGKD 106
           L  I +++FV R +GE E  I+E E G  +G HRG+WF+ IGQ +GL L GGPW+V+ KD
Sbjct: 194 LGKINYNDFVRRFLGEREGAIIELETGKKVGTHRGYWFHTIGQRKGLGLSGGPWFVIKKD 253

Query: 107 RKNN 111
            + N
Sbjct: 254 VEEN 257

[Close]
BLAST of Spg028141 vs. ExPASy TrEMBL
Match: A0A6J1JTU9 (54S ribosomal protein L17, mitochondrial-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111488836 PE=4 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 4.2e-30
Identity = 68/77 (88.31%), Postives = 73/77 (94.81%), Query Frame = 0 

Query: 109 NNFKMQRSFCLAGQLVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQ 168
           + F+MQRSF LA QL KRRGFSTNS+KIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQ
Sbjct: 41  SQFRMQRSFSLASQLAKRRGFSTNSEKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQ 100

Query: 169 QYRRKYPDKFLDKANAR 186
           QYRR+YPD+FLDKANAR
Sbjct: 101 QYRRRYPDEFLDKANAR 117

[Close]
BLAST of Spg028141 vs. ExPASy TrEMBL
Match: A0A6J1HPC7 (39S ribosomal protein L46, mitochondrial-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466099 PE=4 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 1.2e-29
Identity = 68/74 (91.89%), Postives = 72/74 (97.30%), Query Frame = 0 

Query: 112 KMQRSFCLAGQLVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYR 171
           +MQ SF LAGQLVKRRGFSTNS+KIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYR
Sbjct: 9   RMQSSFSLAGQLVKRRGFSTNSEKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYR 68

Query: 172 RKYPDKFLDKANAR 186
           R+YPD+FLDKANAR
Sbjct: 69  RRYPDEFLDKANAR 82

[Close]
BLAST of Spg028141 vs. ExPASy TrEMBL
Match: A0A6J1EEA2 (39S ribosomal protein L46, mitochondrial-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111432442 PE=4 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 1.2e-29
Identity = 68/74 (91.89%), Postives = 72/74 (97.30%), Query Frame = 0 

Query: 112 KMQRSFCLAGQLVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYR 171
           +MQ SF LAGQLVKRRGFSTNS+KIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYR
Sbjct: 9   RMQSSFSLAGQLVKRRGFSTNSEKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYR 68

Query: 172 RKYPDKFLDKANAR 186
           R+YPD+FLDKANAR
Sbjct: 69  RRYPDEFLDKANAR 82

[Close]
BLAST of Spg028141 vs. ExPASy TrEMBL
Match: A0A6J1HRN8 (39S ribosomal protein L46, mitochondrial-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111466099 PE=4 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 2.1e-29
Identity = 68/73 (93.15%), Postives = 71/73 (97.26%), Query Frame = 0 

Query: 113 MQRSFCLAGQLVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRR 172
           MQ SF LAGQLVKRRGFSTNS+KIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRR
Sbjct: 1   MQSSFSLAGQLVKRRGFSTNSEKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRR 60

Query: 173 KYPDKFLDKANAR 186
           +YPD+FLDKANAR
Sbjct: 61  RYPDEFLDKANAR 73

[Close]
BLAST of Spg028141 vs. ExPASy TrEMBL
Match: A0A6J1EB49 (39S ribosomal protein L46, mitochondrial-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111432442 PE=4 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 2.1e-29
Identity = 68/73 (93.15%), Postives = 71/73 (97.26%), Query Frame = 0 

Query: 113 MQRSFCLAGQLVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRR 172
           MQ SF LAGQLVKRRGFSTNS+KIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRR
Sbjct: 1   MQSSFSLAGQLVKRRGFSTNSEKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRR 60

Query: 173 KYPDKFLDKANAR 186
           +YPD+FLDKANAR
Sbjct: 61  RYPDEFLDKANAR 73

[Close]
BLAST of Spg028141 vs. TAIR 10
Match: AT1G51310.1 (transferases;tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferases )

HSP 1 Score: 110.2 bits (274), Expect = 2.6e-24
Identity = 50/64 (78.12%), Postives = 53/64 (82.81%), Query Frame = 0 

Query: 47  LDDIKFSEFVARHIGEEECVILEAELGNFLGKHRGFWFYVIGQCQGLRLPGGPWYVVGKD 106
           L  IKFS+FV RHIGE E +ILEAE G+FLG HRGFWFY IGQ QGLRLPGGPWYVV KD
Sbjct: 284 LGKIKFSDFVCRHIGEMEGIILEAESGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKD 343

Query: 107 RKNN 111
            KNN
Sbjct: 344 TKNN 347

[Close]
BLAST of Spg028141 vs. TAIR 10
Match: AT1G14620.1 (decoy )

HSP 1 Score: 99.4 bits (246), Expect = 4.6e-21
Identity = 49/63 (77.78%), Postives = 53/63 (84.13%), Query Frame = 0 

Query: 123 LVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRRKYPDKFLDKA 182
           L  RRGF T+SDKIVASVLFERL VVIPK DP VYAFQEFKF WQQQ+RR+YPD+FLD A
Sbjct: 14  LESRRGFCTSSDKIVASVLFERLRVVIPKPDPAVYAFQEFKFNWQQQFRRRYPDEFLDIA 73

Query: 183 NAR 186
             R
Sbjct: 74  KNR 76

[Close]
BLAST of Spg028141 vs. TAIR 10
Match: AT1G14620.2 (decoy )

HSP 1 Score: 99.4 bits (246), Expect = 4.6e-21
Identity = 49/63 (77.78%), Postives = 53/63 (84.13%), Query Frame = 0 

Query: 123 LVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRRKYPDKFLDKA 182
           L  RRGF T+SDKIVASVLFERL VVIPK DP VYAFQEFKF WQQQ+RR+YPD+FLD A
Sbjct: 14  LESRRGFCTSSDKIVASVLFERLRVVIPKPDPAVYAFQEFKFNWQQQFRRRYPDEFLDIA 73

Query: 183 NAR 186
             R
Sbjct: 74  KNR 76

The following BLAST results are available for this feature:
BLAST of Spg028141 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022992541.18.6e-3088.3154S ribosomal protein L17, mitochondrial-like isoform X1 [Cucurbita maxima][more]
KAG7027644.11.5e-2990.67hypothetical protein SDJN02_11659 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6595682.11.5e-2990.6739S ribosomal protein L46, mitochondrial, partial [Cucurbita argyrosperma subsp.... [more]
XP_022966446.12.5e-2991.8939S ribosomal protein L46, mitochondrial-like isoform X1 [Cucurbita maxima][more]
XP_022925098.12.5e-2991.8939S ribosomal protein L46, mitochondrial-like isoform X2 [Cucurbita moschata][more]
BLAST of Spg028141 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
B2RHP03.4e-1348.44tRNA-specific 2-thiouridylase MnmA OS=Porphyromonas gingivalis (strain ATCC 3327... [more]
Q7MAW93.4e-1348.44tRNA-specific 2-thiouridylase MnmA OS=Porphyromonas gingivalis (strain ATCC BAA-... [more]
Q5LFN54.4e-1350.00tRNA-specific 2-thiouridylase MnmA 2 OS=Bacteroides fragilis (strain ATCC 25285 ... [more]
Q64WG84.4e-1350.00tRNA-specific 2-thiouridylase MnmA 2 OS=Bacteroides fragilis (strain YCH46) OX=2... [more]
Q8ABF55.8e-1350.00tRNA-specific 2-thiouridylase MnmA 1 OS=Bacteroides thetaiotaomicron (strain ATC... [more]
BLAST of Spg028141 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1JTU94.2e-3088.3154S ribosomal protein L17, mitochondrial-like isoform X1 OS=Cucurbita maxima OX=... [more]
A0A6J1HPC71.2e-2991.8939S ribosomal protein L46, mitochondrial-like isoform X1 OS=Cucurbita maxima OX=... [more]
A0A6J1EEA21.2e-2991.8939S ribosomal protein L46, mitochondrial-like isoform X2 OS=Cucurbita moschata O... [more]
A0A6J1HRN82.1e-2993.1539S ribosomal protein L46, mitochondrial-like isoform X2 OS=Cucurbita maxima OX=... [more]
A0A6J1EB492.1e-2993.1539S ribosomal protein L46, mitochondrial-like isoform X3 OS=Cucurbita moschata O... [more]
BLAST of Spg028141 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G51310.12.6e-2478.13transferases;tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferases [more]
AT1G14620.14.6e-2177.78decoy [more]
AT1G14620.24.6e-2177.78decoy [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF03054tRNA_Me_transcoord: 47..110
e-value: 5.8E-14
score: 51.7
NoneNo IPR availablePANTHERPTHR43052FAMILY NOT NAMEDcoord: 46..113
NoneNo IPR availablePANTHERPTHR43052:SF3BNAA08G29550D PROTEINcoord: 46..113
IPR023382Adenine nucleotide alpha hydrolase-like domainsGENE3D2.30.30.280coord: 59..111
e-value: 8.1E-12
score: 46.3

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg028141.1Spg028141.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005840 ribosome
molecular_function GO:0003735 structural constituent of ribosome
molecular_function GO:0016783 sulfurtransferase activity