Spg028107 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg028107
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionS-adenosyl-L-methionine-dependent methyltransferases superfamily protein
Locationscaffold2: 42254961 .. 42256376 (-)
RNA-Seq ExpressionSpg028107
SyntenySpg028107
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGAAGCTAATCCACACAAAATGGAGGCTAGGAAGAACAATGGGTTGGCTTCAAATTCTATTAGGAGGGCTTGTTATCATCATGACCACATTAAGCCTCTTCAAGTTCTACTCAGCTGGATTCATCTTCCACAAAGAGGACGTTTGCCGGTACTTCTACACGACAACAAGAAACGTATACGAGGCCAGTTTCGACGCAAAAGCATTGTCGGATCGAGTCGAAGAAGTGCTAGATCAGCTAGAAAGTTTGCAAGAAAAGCTCGAATCCACGGTCGAAGAAATGGAAAAGAACAAGCAAGTGTTGAGGAATGGCAATATCACAAGGCTTGAGCATAAAAAGTACTTGGAAGAAGAGGTGATTAAGCCTCTTTATAATGCTCATATTGCCTTGAGGCAAATCAGATTACCAAAGGTTGAAAAAGTTGATCAAAATTCTACCATGAAAGAGGAGCCTTTGATCAACAATTTTGTGGTTGAGGAAATAAGAAAGTATATTTCTCCAAAGAAAAGCAGAATTGGGAAGCTCAATATGTATATGACTGAGAATATTTACAACACAATTGGTCATGCTTGTGTCTTACATAGGAAGGAATTGGAAGAGTATATGGATTATGACATTGGTTCATATTGTAAAGATGATTGGAACTTGGCTCAGAAGCTAATGCTCAATGGTTGCGATCCGTTGCCCCGTCGACGATGCCTGGCAAGAGCGTCAAAGGTCTACCAAAAGCCGTATCCGGTGAACGAATCGCTATGGAAGTCGCCCGACGATCGGAATGTAAGATGGAGTAACTATCAATGTAGGAACTTTTCTTGCTTGTCAAGCAAGAATCCCAGAAGGGGTTACAACAAGTGCAGTGGTTGCTTTGAAATGGAGAAAGAGAAGCTGAAATGGGTCACCAATAGTTCTCTCCCAATCGATTTCGCGATATCGGATGTTTTGACGATCAAGCCAGGGGAGATTCGGATCGGGTTGGATTTCGGTATCGGCACAGGGAGTTTTGCTGCAAGAATGAGAGAGCAAAATGTCACAATTGTGACCACAGCTTTGAACCTTGGGGCTCCTTTCAATGAGTTTATTGCTTTAAGAGGTTTGATTCCTCTGTATGTGACACTAAACCAACGCCTTCCTCTGTTTGATAACACAATGGACTTGATTCACACAACTGGTTTCATGGATGGTTGGCTGGACATGCTACTGTTGGATTTCATACTTTTCGATTGGGATCGGGTTCTACGGCCGGGAGGACTGCTATGGATCGACCGATTCTTTTGCGATCGGAAGGATCTCGACGACTACATGTACATGTTTCTGCAGTTTAGATACAAGAAACACAAATGGGCTATTGCTCCTAAATCAAAAAATGAAGTCTACCTTTCTGCACTGTTGGAAAAACCTCCAAGGGCAATATGA

mRNA sequence

ATGAGGAAGCTAATCCACACAAAATGGAGGCTAGGAAGAACAATGGGTTGGCTTCAAATTCTATTAGGAGGGCTTGTTATCATCATGACCACATTAAGCCTCTTCAAGTTCTACTCAGCTGGATTCATCTTCCACAAAGAGGACGTTTGCCGGTACTTCTACACGACAACAAGAAACGTATACGAGGCCAGTTTCGACGCAAAAGCATTGTCGGATCGAGTCGAAGAAGTGCTAGATCAGCTAGAAAGTTTGCAAGAAAAGCTCGAATCCACGGTCGAAGAAATGGAAAAGAACAAGCAAGTGTTGAGGAATGGCAATATCACAAGGCTTGAGCATAAAAAGTACTTGGAAGAAGAGGTGATTAAGCCTCTTTATAATGCTCATATTGCCTTGAGGCAAATCAGATTACCAAAGGTTGAAAAAGTTGATCAAAATTCTACCATGAAAGAGGAGCCTTTGATCAACAATTTTGTGGTTGAGGAAATAAGAAAGTATATTTCTCCAAAGAAAAGCAGAATTGGGAAGCTCAATATGTATATGACTGAGAATATTTACAACACAATTGGTCATGCTTGTGTCTTACATAGGAAGGAATTGGAAGAGTATATGGATTATGACATTGGTTCATATTGTAAAGATGATTGGAACTTGGCTCAGAAGCTAATGCTCAATGGTTGCGATCCGTTGCCCCGTCGACGATGCCTGGCAAGAGCGTCAAAGGTCTACCAAAAGCCGTATCCGGTGAACGAATCGCTATGGAAGTCGCCCGACGATCGGAATGTAAGATGGAGTAACTATCAATGTAGGAACTTTTCTTGCTTGTCAAGCAAGAATCCCAGAAGGGGTTACAACAAGTGCAGTGGTTGCTTTGAAATGGAGAAAGAGAAGCTGAAATGGGTCACCAATAGTTCTCTCCCAATCGATTTCGCGATATCGGATGTTTTGACGATCAAGCCAGGGGAGATTCGGATCGGGTTGGATTTCGGTATCGGCACAGGGAGTTTTGCTGCAAGAATGAGAGAGCAAAATGTCACAATTGTGACCACAGCTTTGAACCTTGGGGCTCCTTTCAATGAGTTTATTGCTTTAAGAGGTTTGATTCCTCTGTATGTGACACTAAACCAACGCCTTCCTCTGTTTGATAACACAATGGACTTGATTCACACAACTGGTTTCATGGATGGTTGGCTGGACATGCTACTGTTGGATTTCATACTTTTCGATTGGGATCGGGTTCTACGGCCGGGAGGACTGCTATGGATCGACCGATTCTTTTGCGATCGGAAGGATCTCGACGACTACATGTACATGTTTCTGCAGTTTAGATACAAGAAACACAAATGGGCTATTGCTCCTAAATCAAAAAATGAAGTCTACCTTTCTGCACTGTTGGAAAAACCTCCAAGGGCAATATGA

Coding sequence (CDS)

ATGAGGAAGCTAATCCACACAAAATGGAGGCTAGGAAGAACAATGGGTTGGCTTCAAATTCTATTAGGAGGGCTTGTTATCATCATGACCACATTAAGCCTCTTCAAGTTCTACTCAGCTGGATTCATCTTCCACAAAGAGGACGTTTGCCGGTACTTCTACACGACAACAAGAAACGTATACGAGGCCAGTTTCGACGCAAAAGCATTGTCGGATCGAGTCGAAGAAGTGCTAGATCAGCTAGAAAGTTTGCAAGAAAAGCTCGAATCCACGGTCGAAGAAATGGAAAAGAACAAGCAAGTGTTGAGGAATGGCAATATCACAAGGCTTGAGCATAAAAAGTACTTGGAAGAAGAGGTGATTAAGCCTCTTTATAATGCTCATATTGCCTTGAGGCAAATCAGATTACCAAAGGTTGAAAAAGTTGATCAAAATTCTACCATGAAAGAGGAGCCTTTGATCAACAATTTTGTGGTTGAGGAAATAAGAAAGTATATTTCTCCAAAGAAAAGCAGAATTGGGAAGCTCAATATGTATATGACTGAGAATATTTACAACACAATTGGTCATGCTTGTGTCTTACATAGGAAGGAATTGGAAGAGTATATGGATTATGACATTGGTTCATATTGTAAAGATGATTGGAACTTGGCTCAGAAGCTAATGCTCAATGGTTGCGATCCGTTGCCCCGTCGACGATGCCTGGCAAGAGCGTCAAAGGTCTACCAAAAGCCGTATCCGGTGAACGAATCGCTATGGAAGTCGCCCGACGATCGGAATGTAAGATGGAGTAACTATCAATGTAGGAACTTTTCTTGCTTGTCAAGCAAGAATCCCAGAAGGGGTTACAACAAGTGCAGTGGTTGCTTTGAAATGGAGAAAGAGAAGCTGAAATGGGTCACCAATAGTTCTCTCCCAATCGATTTCGCGATATCGGATGTTTTGACGATCAAGCCAGGGGAGATTCGGATCGGGTTGGATTTCGGTATCGGCACAGGGAGTTTTGCTGCAAGAATGAGAGAGCAAAATGTCACAATTGTGACCACAGCTTTGAACCTTGGGGCTCCTTTCAATGAGTTTATTGCTTTAAGAGGTTTGATTCCTCTGTATGTGACACTAAACCAACGCCTTCCTCTGTTTGATAACACAATGGACTTGATTCACACAACTGGTTTCATGGATGGTTGGCTGGACATGCTACTGTTGGATTTCATACTTTTCGATTGGGATCGGGTTCTACGGCCGGGAGGACTGCTATGGATCGACCGATTCTTTTGCGATCGGAAGGATCTCGACGACTACATGTACATGTTTCTGCAGTTTAGATACAAGAAACACAAATGGGCTATTGCTCCTAAATCAAAAAATGAAGTCTACCTTTCTGCACTGTTGGAAAAACCTCCAAGGGCAATATGA

Protein sequence

MRKLIHTKWRLGRTMGWLQILLGGLVIIMTTLSLFKFYSAGFIFHKEDVCRYFYTTTRNVYEASFDAKALSDRVEEVLDQLESLQEKLESTVEEMEKNKQVLRNGNITRLEHKKYLEEEVIKPLYNAHIALRQIRLPKVEKVDQNSTMKEEPLINNFVVEEIRKYISPKKSRIGKLNMYMTENIYNTIGHACVLHRKELEEYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLARASKVYQKPYPVNESLWKSPDDRNVRWSNYQCRNFSCLSSKNPRRGYNKCSGCFEMEKEKLKWVTNSSLPIDFAISDVLTIKPGEIRIGLDFGIGTGSFAARMREQNVTIVTTALNLGAPFNEFIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGLLWIDRFFCDRKDLDDYMYMFLQFRYKKHKWAIAPKSKNEVYLSALLEKPPRAI
Homology
BLAST of Spg028107 vs. NCBI nr
Match: XP_038880508.1 (uncharacterized protein LOC120072172 [Benincasa hispida])

HSP 1 Score: 896.0 bits (2314), Expect = 1.4e-256
Identity = 440/468 (94.02%), Postives = 451/468 (96.37%), Query Frame = 0

Query: 5   IHTKWRLGRTMGWLQILLGGLVIIMTTLSLFKFYSAGFIFHKEDVCRYFYTTTRNVYEAS 64
           +  K RLGRTMGWLQILLGGLVII+TTLSLFKFYSAGFIFHK+DVCRYFYTTTR+VYE S
Sbjct: 1   MRNKGRLGRTMGWLQILLGGLVIIITTLSLFKFYSAGFIFHKDDVCRYFYTTTRHVYEES 60

Query: 65  FDAKALSDRVEEVLDQLESLQEKLESTVEEMEKNKQVLRNGNITRLEHKKYLEEEVIKPL 124
           FDAKALSDRVEEVLDQLESLQEKLES VEEM KNKQVL NGNITR+EHKKYLEEEVIKPL
Sbjct: 61  FDAKALSDRVEEVLDQLESLQEKLESAVEEMGKNKQVLNNGNITRIEHKKYLEEEVIKPL 120

Query: 125 YNAHIALRQIRLPKVEKVDQN-STMKEEPLINNFVVEEIRKYISPKKSRIGKLNMYMTEN 184
           YNAHIALRQIRLPKVEK  QN ST+KEEPLINNFVVEEIRKYISPKKSRIGKLNMYMTEN
Sbjct: 121 YNAHIALRQIRLPKVEKNGQNYSTVKEEPLINNFVVEEIRKYISPKKSRIGKLNMYMTEN 180

Query: 185 IYNTIGHACVLHRKELEEYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLARASKVYQ 244
           IYNTIGHACVLHRKELE+YMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCL RASKVYQ
Sbjct: 181 IYNTIGHACVLHRKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRASKVYQ 240

Query: 245 KPYPVNESLWKSPDDRNVRWSNYQCRNFSCLSSKNPRRGYNKCSGCFEMEKEKLKWVTNS 304
           KPYPVNESLWKSPDDRNVRWSNYQCRNFSCLSSKNP+RGYNKCSGCFE++KEKLKWV NS
Sbjct: 241 KPYPVNESLWKSPDDRNVRWSNYQCRNFSCLSSKNPKRGYNKCSGCFEIDKEKLKWVINS 300

Query: 305 SLPIDFAISDVLTIKPGEIRIGLDFGIGTGSFAARMREQNVTIVTTALNLGAPFNEFIAL 364
           SL +DF ISDVL IKPGEIRIGLDFGI TGSFAARMREQNVTIVTTALNLGAPFNEFIAL
Sbjct: 301 SLAVDFTISDVLAIKPGEIRIGLDFGISTGSFAARMREQNVTIVTTALNLGAPFNEFIAL 360

Query: 365 RGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGLLWIDR 424
           RGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGLLWIDR
Sbjct: 361 RGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGLLWIDR 420

Query: 425 FFCDRKDLDDYMYMFLQFRYKKHKWAIAPKSKNEVYLSALLEKPPRAI 472
           FFCDRKDLDDYMYMFLQFRYKKHKW IAPKSKNEVYLSALLEKPPRAI
Sbjct: 421 FFCDRKDLDDYMYMFLQFRYKKHKWTIAPKSKNEVYLSALLEKPPRAI 468

BLAST of Spg028107 vs. NCBI nr
Match: XP_022146046.1 (uncharacterized protein LOC111015349 [Momordica charantia])

HSP 1 Score: 890.6 bits (2300), Expect = 6.0e-255
Identity = 436/472 (92.37%), Postives = 454/472 (96.19%), Query Frame = 0

Query: 1   MRKLIHTKWRLGRTMGWLQILLGGLVIIMTTLSLFKFYSAGFIFHKEDVCRYFYTTTRNV 60
           MRKLIHTK RLGRTMGWLQILLGGLVI++TTLSLFKFYSAGF FHKEDVCRYFY TT++V
Sbjct: 1   MRKLIHTKCRLGRTMGWLQILLGGLVILITTLSLFKFYSAGFFFHKEDVCRYFY-TTKHV 60

Query: 61  YEASFDAKALSDRVEEVLDQLESLQEKLESTVEEMEKNKQVLRNGNITRLEHKKYLEEEV 120
           YEASFD KALSDRVEEVLDQL+ LQ KLES VEEMEKNKQVL NG+ITRLEHKKYLEEEV
Sbjct: 61  YEASFDVKALSDRVEEVLDQLDGLQAKLESAVEEMEKNKQVLSNGSITRLEHKKYLEEEV 120

Query: 121 IKPLYNAHIALRQIRLPKVEKV-DQNSTMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180
           I+PLYNAHIALRQIRLPKVEK+ DQNS M+EEPLINNFVVEEIRKYISPKKSRIGKLNMY
Sbjct: 121 IRPLYNAHIALRQIRLPKVEKIDDQNSNMQEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180

Query: 181 MTENIYNTIGHACVLHRKELEEYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLARAS 240
           MTE IYNTIGHACVLHRKELE+YMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLARAS
Sbjct: 181 MTEKIYNTIGHACVLHRKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLARAS 240

Query: 241 KVYQKPYPVNESLWKSPDDRNVRWSNYQCRNFSCLSSKNPRRGYNKCSGCFEMEKEKLKW 300
           KVYQ+PYPVNESLWK PDDRNVRWSNYQCRNFSCLSSKNPRRGYNKCSGCFE+EKEK KW
Sbjct: 241 KVYQRPYPVNESLWKLPDDRNVRWSNYQCRNFSCLSSKNPRRGYNKCSGCFELEKEKRKW 300

Query: 301 VTNSSLPIDFAISDVLTIKPGEIRIGLDFGIGTGSFAARMREQNVTIVTTALNLGAPFNE 360
           VTNSSL +DF +SDVL +K GEIR+GLDFGI +GSFAARM+EQNVTIVTTALNLGAPFNE
Sbjct: 301 VTNSSLSVDFVLSDVLALKAGEIRVGLDFGISSGSFAARMKEQNVTIVTTALNLGAPFNE 360

Query: 361 FIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGLL 420
           FIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGLL
Sbjct: 361 FIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGLL 420

Query: 421 WIDRFFCDRKDLDDYMYMFLQFRYKKHKWAIAPKSKNEVYLSALLEKPPRAI 472
           WIDRFFCDRKDLDDYMYMFLQFRYKKHKWAIAPKSKNEVYLSALLEKPPRAI
Sbjct: 421 WIDRFFCDRKDLDDYMYMFLQFRYKKHKWAIAPKSKNEVYLSALLEKPPRAI 471

BLAST of Spg028107 vs. NCBI nr
Match: XP_011659893.1 (uncharacterized protein LOC101207467 [Cucumis sativus] >KAE8653367.1 hypothetical protein Csa_006971 [Cucumis sativus])

HSP 1 Score: 881.7 bits (2277), Expect = 2.8e-252
Identity = 434/473 (91.75%), Postives = 452/473 (95.56%), Query Frame = 0

Query: 1   MRKLIHTKWRLGRTMGWLQILLGGLVIIMTTLSLFKFYSAGFIFHKEDVCRYFYTTTRNV 60
           M+KL HTK RLGRTMGWLQILL GLVIIMTTLSLFKFYSAGFIFHK+DVCRYFYTTTR+ 
Sbjct: 1   MKKLFHTKGRLGRTMGWLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHA 60

Query: 61  YEASFDAKALSDRVEEVLDQLESLQEKLESTVEEMEKNKQVLRNGNITRLEHKKYLEEEV 120
           YE +FDAKALS+RVEEVLDQLESLQEKLE TVEEM KNKQVL NG IT +EHKKYLE+EV
Sbjct: 61  YEETFDAKALSNRVEEVLDQLESLQEKLEFTVEEMGKNKQVLNNGKITMIEHKKYLEDEV 120

Query: 121 IKPLYNAHIALRQIRLPKVEKVDQNST-MKEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180
           IKPLYNAHIALRQIRLPKVEK  QNS+ MKEEPLINNFVVEEIRKYISPKKSRIGKLNMY
Sbjct: 121 IKPLYNAHIALRQIRLPKVEKNGQNSSAMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180

Query: 181 MTENIYNTIGHACVLHRKELEEYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLARAS 240
           MTENIYNTIGHACVL+RKELE+YMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCL RAS
Sbjct: 181 MTENIYNTIGHACVLYRKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRAS 240

Query: 241 KVYQKPYPVNESLWKSPDDRNVRWSNYQCRNFSCLSSKNPRRGYNKCSGCFEMEKEKLKW 300
           KVYQKPYP+NESLWK PDDRNVRWSNYQCRNFSCLSS NP++GYNKCSGCFEMEKEKLKW
Sbjct: 241 KVYQKPYPLNESLWKMPDDRNVRWSNYQCRNFSCLSSNNPKKGYNKCSGCFEMEKEKLKW 300

Query: 301 V-TNSSLPIDFAISDVLTIKPGEIRIGLDFGIGTGSFAARMREQNVTIVTTALNLGAPFN 360
           V +NSSL +DFAISDVL IKPGEIRIGLDFGI TGSFAARMRE+NVTIVTTALNLGAPFN
Sbjct: 301 VISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFN 360

Query: 361 EFIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGL 420
           EFIALRGL PLY+TLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGL
Sbjct: 361 EFIALRGLFPLYLTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGL 420

Query: 421 LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWAIAPKSKNEVYLSALLEKPPRAI 472
           LWIDRFFCDRKDLDDYMYMFLQFRYKKHKW+IAPKSKNEVYLSALLEKPPRAI
Sbjct: 421 LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSKNEVYLSALLEKPPRAI 473

BLAST of Spg028107 vs. NCBI nr
Match: XP_008450643.1 (PREDICTED: uncharacterized protein LOC103492170 [Cucumis melo] >KAA0050891.1 putative Methyltransferase [Cucumis melo var. makuwa] >TYK10243.1 putative Methyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 880.2 bits (2273), Expect = 8.1e-252
Identity = 434/473 (91.75%), Postives = 454/473 (95.98%), Query Frame = 0

Query: 1   MRKLIHTKWRLGRTMGWLQILLGGLVIIMTTLSLFKFYSAGFIFHKEDVCRYFYTTTRNV 60
           M+KL HTK RLGRTMG LQILL GLVIIMTTLSLFKFYSAGFIFHK+DVCRYFYTTTR+V
Sbjct: 1   MKKLFHTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHV 60

Query: 61  YEASFDAKALSDRVEEVLDQLESLQEKLESTVEEMEKNKQVLRNGNITRLEHKKYLEEEV 120
           YE +FDAKALS+RVEEVLDQLESLQEKLES VEEMEKNKQVL NGNI  +EHKKYLE+EV
Sbjct: 61  YEETFDAKALSNRVEEVLDQLESLQEKLESAVEEMEKNKQVLNNGNIRMIEHKKYLEDEV 120

Query: 121 IKPLYNAHIALRQIRLPKVEKVDQN-STMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180
           IKPLYNAHIALRQIRLPKV+K  QN STMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY
Sbjct: 121 IKPLYNAHIALRQIRLPKVDKNGQNSSTMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180

Query: 181 MTENIYNTIGHACVLHRKELEEYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLARAS 240
           MTENIYNTIGHACVL++KELE+YMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCL RAS
Sbjct: 181 MTENIYNTIGHACVLYKKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRAS 240

Query: 241 KVYQKPYPVNESLWKSPDDRNVRWSNYQCRNFSCLSSKNPRRGYNKCSGCFEMEKEKLKW 300
           KVYQKPYP+NESLWK+PDDRNVRWSNYQCRNFSCLSSKNP+RGYNKCSGCFE+EKEKLKW
Sbjct: 241 KVYQKPYPLNESLWKTPDDRNVRWSNYQCRNFSCLSSKNPKRGYNKCSGCFEIEKEKLKW 300

Query: 301 V-TNSSLPIDFAISDVLTIKPGEIRIGLDFGIGTGSFAARMREQNVTIVTTALNLGAPFN 360
           V +NSSL +DFAISDVL IKPGEIRIGLDFGI TGSFAARMRE+NVTIVTTALNLGAPFN
Sbjct: 301 VISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFN 360

Query: 361 EFIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGL 420
           EFIALRGL PLY TLNQRLPLFDNTMDLIHT+GFMDGWLDMLLLDFILFDWDRVLRPGGL
Sbjct: 361 EFIALRGLFPLYSTLNQRLPLFDNTMDLIHTSGFMDGWLDMLLLDFILFDWDRVLRPGGL 420

Query: 421 LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWAIAPKSKNEVYLSALLEKPPRAI 472
           LWIDRFFCDRKDLDDYMYMFLQFRYKKHKW+IAPKSKNEVYLSALLEKPPRAI
Sbjct: 421 LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSKNEVYLSALLEKPPRAI 473

BLAST of Spg028107 vs. NCBI nr
Match: XP_022969573.1 (uncharacterized protein LOC111468555 [Cucurbita maxima])

HSP 1 Score: 871.3 bits (2250), Expect = 3.8e-249
Identity = 422/473 (89.22%), Postives = 450/473 (95.14%), Query Frame = 0

Query: 1   MRKLIHTKWRLGRTMGWLQILLGGLVIIMTTLSLFKFYSAGFIFHKEDVCRYFY--TTTR 60
           MRKLIHTKWR+GRTMG LQILLGGLVI +TTLSLFKFYSAGFIFHKEDVCRYFY  TTT+
Sbjct: 1   MRKLIHTKWRVGRTMGCLQILLGGLVITITTLSLFKFYSAGFIFHKEDVCRYFYTTTTTK 60

Query: 61  NVYEASFDAKALSDRVEEVLDQLESLQEKLESTVEEMEKNKQVLRNGNITRLEHKKYLEE 120
            +YEASFDAKALSDRVEEVLDQLESLQ+KLES VEEME++KQ L NGN+ R+EHKKYLE 
Sbjct: 61  QIYEASFDAKALSDRVEEVLDQLESLQDKLESVVEEMERDKQALSNGNMRRIEHKKYLEM 120

Query: 121 EVIKPLYNAHIALRQIRLPKVEKVDQNSTMKEEPLINNFVVEEIRKYISPKKSRIGKLNM 180
           EVIKPLYNAHI LRQIRLPKVEK+++NS+MKEEPLINNFVVEEIRKYI+PKKSR GK+NM
Sbjct: 121 EVIKPLYNAHIGLRQIRLPKVEKMNENSSMKEEPLINNFVVEEIRKYITPKKSRTGKVNM 180

Query: 181 YMTENIYNTIGHACVLHRKELEEYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLARA 240
           YMTE+IYNTIGHACVL RKELEEYM YDIGSYCKDDWN+ QKLMLNGCDPLPRRRCL RA
Sbjct: 181 YMTESIYNTIGHACVLVRKELEEYMSYDIGSYCKDDWNMGQKLMLNGCDPLPRRRCLTRA 240

Query: 241 SKVYQKPYPVNESLWKSPDDRNVRWSNYQCRNFSCLSSKNPRRGYNKCSGCFEMEKEKLK 300
           SKVYQKPYPVNESLWK+PDDRNVRWSNYQCRNFSCLSSKNP+RGYNKCSGCFEMEKEK+K
Sbjct: 241 SKVYQKPYPVNESLWKTPDDRNVRWSNYQCRNFSCLSSKNPKRGYNKCSGCFEMEKEKVK 300

Query: 301 WVTNSSLPIDFAISDVLTIKPGEIRIGLDFGIGTGSFAARMREQNVTIVTTALNLGAPFN 360
           WV NSS+ +DF ISDVL +K GEIR+GLDFGI TGSF ARMRE+NVTIVTTALNLGAPFN
Sbjct: 301 WVRNSSVAVDFRISDVLGMKAGEIRVGLDFGISTGSFGARMREENVTIVTTALNLGAPFN 360

Query: 361 EFIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGL 420
           EFIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGW+DMLLLDFILFDWDRVLRPGGL
Sbjct: 361 EFIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWVDMLLLDFILFDWDRVLRPGGL 420

Query: 421 LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWAIAPKSKNEVYLSALLEKPPRAI 472
           LWIDRFFCDRKDLDDY+YMFLQFRYKKHKWAIAPKSKNEVYLSA+LEKPPRAI
Sbjct: 421 LWIDRFFCDRKDLDDYIYMFLQFRYKKHKWAIAPKSKNEVYLSAVLEKPPRAI 473

BLAST of Spg028107 vs. ExPASy Swiss-Prot
Match: Q8RWB7 (Probable methyltransferase At1g29790 OS=Arabidopsis thaliana OX=3702 GN=CLSC5 PE=2 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 1.0e-55
Identity = 122/287 (42.51%), Postives = 175/287 (60.98%), Query Frame = 0

Query: 188 IGHACVLHRKELEEYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLARASKVYQKPYP 247
           I  AC  +   L EYM+Y   S C  D +L +KL+L GC PLPRRRC +R         P
Sbjct: 105 IASACHNYPDLLHEYMNYTPFSLCPSDTDLVEKLILRGCHPLPRRRCFSRT--------P 164

Query: 248 VNESLWKSPDDRNVRWSNYQCRNFSCLSSKNPRRGYNKCSGCFEMEKEKLKWVTNSSLPI 307
            N S   S  + NV WS Y C++F CL +K    G++       +EK K ++    S  +
Sbjct: 165 RNPS--DSKPESNVLWSYYSCKSFDCLITKFSDLGFD-----LSLEKSKSQFSAYKS-EL 224

Query: 308 DFAISDVLTIKPGE---IRIGLDFGIGTGSFAARMREQNVTIVTTALNLGAPFNEFIALR 367
           D  IS +L I       +R+G+D G GTGSFAA M+ +NVT++TT +N  AP++E +A+R
Sbjct: 225 DLPISQLLQIAKSANSVLRLGIDVGGGTGSFAAAMKARNVTVLTTTMNFNAPYSEAVAMR 284

Query: 368 GLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGLLWIDRF 427
           GL+PL+V L QRLP+FD  +DL+     ++ W+ + +++F  FD DR+LR GG LW+DRF
Sbjct: 285 GLVPLHVPLQQRLPVFDGVVDLVRCGRAVNRWIPVTVMEFFFFDLDRILRGGGYLWLDRF 344

Query: 428 FCDRKDLDD-YMYMFLQFRYKKHKWAIAPKSKN---EVYLSALLEKP 468
           F  + DL++ Y  M  +  YKK KWA+A K+ +   EV+L+ALL+KP
Sbjct: 345 FSKKVDLENVYAPMIGKLGYKKVKWAVANKADSKHGEVFLTALLQKP 375

BLAST of Spg028107 vs. ExPASy TrEMBL
Match: A0A6J1CY71 (uncharacterized protein LOC111015349 OS=Momordica charantia OX=3673 GN=LOC111015349 PE=3 SV=1)

HSP 1 Score: 890.6 bits (2300), Expect = 2.9e-255
Identity = 436/472 (92.37%), Postives = 454/472 (96.19%), Query Frame = 0

Query: 1   MRKLIHTKWRLGRTMGWLQILLGGLVIIMTTLSLFKFYSAGFIFHKEDVCRYFYTTTRNV 60
           MRKLIHTK RLGRTMGWLQILLGGLVI++TTLSLFKFYSAGF FHKEDVCRYFY TT++V
Sbjct: 1   MRKLIHTKCRLGRTMGWLQILLGGLVILITTLSLFKFYSAGFFFHKEDVCRYFY-TTKHV 60

Query: 61  YEASFDAKALSDRVEEVLDQLESLQEKLESTVEEMEKNKQVLRNGNITRLEHKKYLEEEV 120
           YEASFD KALSDRVEEVLDQL+ LQ KLES VEEMEKNKQVL NG+ITRLEHKKYLEEEV
Sbjct: 61  YEASFDVKALSDRVEEVLDQLDGLQAKLESAVEEMEKNKQVLSNGSITRLEHKKYLEEEV 120

Query: 121 IKPLYNAHIALRQIRLPKVEKV-DQNSTMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180
           I+PLYNAHIALRQIRLPKVEK+ DQNS M+EEPLINNFVVEEIRKYISPKKSRIGKLNMY
Sbjct: 121 IRPLYNAHIALRQIRLPKVEKIDDQNSNMQEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180

Query: 181 MTENIYNTIGHACVLHRKELEEYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLARAS 240
           MTE IYNTIGHACVLHRKELE+YMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLARAS
Sbjct: 181 MTEKIYNTIGHACVLHRKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLARAS 240

Query: 241 KVYQKPYPVNESLWKSPDDRNVRWSNYQCRNFSCLSSKNPRRGYNKCSGCFEMEKEKLKW 300
           KVYQ+PYPVNESLWK PDDRNVRWSNYQCRNFSCLSSKNPRRGYNKCSGCFE+EKEK KW
Sbjct: 241 KVYQRPYPVNESLWKLPDDRNVRWSNYQCRNFSCLSSKNPRRGYNKCSGCFELEKEKRKW 300

Query: 301 VTNSSLPIDFAISDVLTIKPGEIRIGLDFGIGTGSFAARMREQNVTIVTTALNLGAPFNE 360
           VTNSSL +DF +SDVL +K GEIR+GLDFGI +GSFAARM+EQNVTIVTTALNLGAPFNE
Sbjct: 301 VTNSSLSVDFVLSDVLALKAGEIRVGLDFGISSGSFAARMKEQNVTIVTTALNLGAPFNE 360

Query: 361 FIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGLL 420
           FIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGLL
Sbjct: 361 FIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGLL 420

Query: 421 WIDRFFCDRKDLDDYMYMFLQFRYKKHKWAIAPKSKNEVYLSALLEKPPRAI 472
           WIDRFFCDRKDLDDYMYMFLQFRYKKHKWAIAPKSKNEVYLSALLEKPPRAI
Sbjct: 421 WIDRFFCDRKDLDDYMYMFLQFRYKKHKWAIAPKSKNEVYLSALLEKPPRAI 471

BLAST of Spg028107 vs. ExPASy TrEMBL
Match: A0A5D3CEF4 (Putative Methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G003900 PE=3 SV=1)

HSP 1 Score: 880.2 bits (2273), Expect = 3.9e-252
Identity = 434/473 (91.75%), Postives = 454/473 (95.98%), Query Frame = 0

Query: 1   MRKLIHTKWRLGRTMGWLQILLGGLVIIMTTLSLFKFYSAGFIFHKEDVCRYFYTTTRNV 60
           M+KL HTK RLGRTMG LQILL GLVIIMTTLSLFKFYSAGFIFHK+DVCRYFYTTTR+V
Sbjct: 1   MKKLFHTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHV 60

Query: 61  YEASFDAKALSDRVEEVLDQLESLQEKLESTVEEMEKNKQVLRNGNITRLEHKKYLEEEV 120
           YE +FDAKALS+RVEEVLDQLESLQEKLES VEEMEKNKQVL NGNI  +EHKKYLE+EV
Sbjct: 61  YEETFDAKALSNRVEEVLDQLESLQEKLESAVEEMEKNKQVLNNGNIRMIEHKKYLEDEV 120

Query: 121 IKPLYNAHIALRQIRLPKVEKVDQN-STMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180
           IKPLYNAHIALRQIRLPKV+K  QN STMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY
Sbjct: 121 IKPLYNAHIALRQIRLPKVDKNGQNSSTMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180

Query: 181 MTENIYNTIGHACVLHRKELEEYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLARAS 240
           MTENIYNTIGHACVL++KELE+YMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCL RAS
Sbjct: 181 MTENIYNTIGHACVLYKKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRAS 240

Query: 241 KVYQKPYPVNESLWKSPDDRNVRWSNYQCRNFSCLSSKNPRRGYNKCSGCFEMEKEKLKW 300
           KVYQKPYP+NESLWK+PDDRNVRWSNYQCRNFSCLSSKNP+RGYNKCSGCFE+EKEKLKW
Sbjct: 241 KVYQKPYPLNESLWKTPDDRNVRWSNYQCRNFSCLSSKNPKRGYNKCSGCFEIEKEKLKW 300

Query: 301 V-TNSSLPIDFAISDVLTIKPGEIRIGLDFGIGTGSFAARMREQNVTIVTTALNLGAPFN 360
           V +NSSL +DFAISDVL IKPGEIRIGLDFGI TGSFAARMRE+NVTIVTTALNLGAPFN
Sbjct: 301 VISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFN 360

Query: 361 EFIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGL 420
           EFIALRGL PLY TLNQRLPLFDNTMDLIHT+GFMDGWLDMLLLDFILFDWDRVLRPGGL
Sbjct: 361 EFIALRGLFPLYSTLNQRLPLFDNTMDLIHTSGFMDGWLDMLLLDFILFDWDRVLRPGGL 420

Query: 421 LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWAIAPKSKNEVYLSALLEKPPRAI 472
           LWIDRFFCDRKDLDDYMYMFLQFRYKKHKW+IAPKSKNEVYLSALLEKPPRAI
Sbjct: 421 LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSKNEVYLSALLEKPPRAI 473

BLAST of Spg028107 vs. ExPASy TrEMBL
Match: A0A1S3BQC0 (uncharacterized protein LOC103492170 OS=Cucumis melo OX=3656 GN=LOC103492170 PE=3 SV=1)

HSP 1 Score: 880.2 bits (2273), Expect = 3.9e-252
Identity = 434/473 (91.75%), Postives = 454/473 (95.98%), Query Frame = 0

Query: 1   MRKLIHTKWRLGRTMGWLQILLGGLVIIMTTLSLFKFYSAGFIFHKEDVCRYFYTTTRNV 60
           M+KL HTK RLGRTMG LQILL GLVIIMTTLSLFKFYSAGFIFHK+DVCRYFYTTTR+V
Sbjct: 1   MKKLFHTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHV 60

Query: 61  YEASFDAKALSDRVEEVLDQLESLQEKLESTVEEMEKNKQVLRNGNITRLEHKKYLEEEV 120
           YE +FDAKALS+RVEEVLDQLESLQEKLES VEEMEKNKQVL NGNI  +EHKKYLE+EV
Sbjct: 61  YEETFDAKALSNRVEEVLDQLESLQEKLESAVEEMEKNKQVLNNGNIRMIEHKKYLEDEV 120

Query: 121 IKPLYNAHIALRQIRLPKVEKVDQN-STMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180
           IKPLYNAHIALRQIRLPKV+K  QN STMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY
Sbjct: 121 IKPLYNAHIALRQIRLPKVDKNGQNSSTMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180

Query: 181 MTENIYNTIGHACVLHRKELEEYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLARAS 240
           MTENIYNTIGHACVL++KELE+YMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCL RAS
Sbjct: 181 MTENIYNTIGHACVLYKKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRAS 240

Query: 241 KVYQKPYPVNESLWKSPDDRNVRWSNYQCRNFSCLSSKNPRRGYNKCSGCFEMEKEKLKW 300
           KVYQKPYP+NESLWK+PDDRNVRWSNYQCRNFSCLSSKNP+RGYNKCSGCFE+EKEKLKW
Sbjct: 241 KVYQKPYPLNESLWKTPDDRNVRWSNYQCRNFSCLSSKNPKRGYNKCSGCFEIEKEKLKW 300

Query: 301 V-TNSSLPIDFAISDVLTIKPGEIRIGLDFGIGTGSFAARMREQNVTIVTTALNLGAPFN 360
           V +NSSL +DFAISDVL IKPGEIRIGLDFGI TGSFAARMRE+NVTIVTTALNLGAPFN
Sbjct: 301 VISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFN 360

Query: 361 EFIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGL 420
           EFIALRGL PLY TLNQRLPLFDNTMDLIHT+GFMDGWLDMLLLDFILFDWDRVLRPGGL
Sbjct: 361 EFIALRGLFPLYSTLNQRLPLFDNTMDLIHTSGFMDGWLDMLLLDFILFDWDRVLRPGGL 420

Query: 421 LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWAIAPKSKNEVYLSALLEKPPRAI 472
           LWIDRFFCDRKDLDDYMYMFLQFRYKKHKW+IAPKSKNEVYLSALLEKPPRAI
Sbjct: 421 LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSKNEVYLSALLEKPPRAI 473

BLAST of Spg028107 vs. ExPASy TrEMBL
Match: A0A6J1I1D3 (uncharacterized protein LOC111468555 OS=Cucurbita maxima OX=3661 GN=LOC111468555 PE=3 SV=1)

HSP 1 Score: 871.3 bits (2250), Expect = 1.8e-249
Identity = 422/473 (89.22%), Postives = 450/473 (95.14%), Query Frame = 0

Query: 1   MRKLIHTKWRLGRTMGWLQILLGGLVIIMTTLSLFKFYSAGFIFHKEDVCRYFY--TTTR 60
           MRKLIHTKWR+GRTMG LQILLGGLVI +TTLSLFKFYSAGFIFHKEDVCRYFY  TTT+
Sbjct: 1   MRKLIHTKWRVGRTMGCLQILLGGLVITITTLSLFKFYSAGFIFHKEDVCRYFYTTTTTK 60

Query: 61  NVYEASFDAKALSDRVEEVLDQLESLQEKLESTVEEMEKNKQVLRNGNITRLEHKKYLEE 120
            +YEASFDAKALSDRVEEVLDQLESLQ+KLES VEEME++KQ L NGN+ R+EHKKYLE 
Sbjct: 61  QIYEASFDAKALSDRVEEVLDQLESLQDKLESVVEEMERDKQALSNGNMRRIEHKKYLEM 120

Query: 121 EVIKPLYNAHIALRQIRLPKVEKVDQNSTMKEEPLINNFVVEEIRKYISPKKSRIGKLNM 180
           EVIKPLYNAHI LRQIRLPKVEK+++NS+MKEEPLINNFVVEEIRKYI+PKKSR GK+NM
Sbjct: 121 EVIKPLYNAHIGLRQIRLPKVEKMNENSSMKEEPLINNFVVEEIRKYITPKKSRTGKVNM 180

Query: 181 YMTENIYNTIGHACVLHRKELEEYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLARA 240
           YMTE+IYNTIGHACVL RKELEEYM YDIGSYCKDDWN+ QKLMLNGCDPLPRRRCL RA
Sbjct: 181 YMTESIYNTIGHACVLVRKELEEYMSYDIGSYCKDDWNMGQKLMLNGCDPLPRRRCLTRA 240

Query: 241 SKVYQKPYPVNESLWKSPDDRNVRWSNYQCRNFSCLSSKNPRRGYNKCSGCFEMEKEKLK 300
           SKVYQKPYPVNESLWK+PDDRNVRWSNYQCRNFSCLSSKNP+RGYNKCSGCFEMEKEK+K
Sbjct: 241 SKVYQKPYPVNESLWKTPDDRNVRWSNYQCRNFSCLSSKNPKRGYNKCSGCFEMEKEKVK 300

Query: 301 WVTNSSLPIDFAISDVLTIKPGEIRIGLDFGIGTGSFAARMREQNVTIVTTALNLGAPFN 360
           WV NSS+ +DF ISDVL +K GEIR+GLDFGI TGSF ARMRE+NVTIVTTALNLGAPFN
Sbjct: 301 WVRNSSVAVDFRISDVLGMKAGEIRVGLDFGISTGSFGARMREENVTIVTTALNLGAPFN 360

Query: 361 EFIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGL 420
           EFIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGW+DMLLLDFILFDWDRVLRPGGL
Sbjct: 361 EFIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWVDMLLLDFILFDWDRVLRPGGL 420

Query: 421 LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWAIAPKSKNEVYLSALLEKPPRAI 472
           LWIDRFFCDRKDLDDY+YMFLQFRYKKHKWAIAPKSKNEVYLSA+LEKPPRAI
Sbjct: 421 LWIDRFFCDRKDLDDYIYMFLQFRYKKHKWAIAPKSKNEVYLSAVLEKPPRAI 473

BLAST of Spg028107 vs. ExPASy TrEMBL
Match: A0A6J1EZZ0 (uncharacterized protein LOC111440913 OS=Cucurbita moschata OX=3662 GN=LOC111440913 PE=3 SV=1)

HSP 1 Score: 865.1 bits (2234), Expect = 1.3e-247
Identity = 422/476 (88.66%), Postives = 448/476 (94.12%), Query Frame = 0

Query: 1   MRKLIHTKWRLGRTMGWLQILLGGLVIIMTTLSLFKFYSAGFIFHKEDVCRYFY-----T 60
           MRKLIHTKWR+GRTMG LQILLGGLVI +TTLSLFKFYSAGFIFHKEDVCRYFY     T
Sbjct: 1   MRKLIHTKWRVGRTMGCLQILLGGLVITITTLSLFKFYSAGFIFHKEDVCRYFYTPPTTT 60

Query: 61  TTRNVYEASFDAKALSDRVEEVLDQLESLQEKLESTVEEMEKNKQVLRNGNITRLEHKKY 120
           TT+ VYEASFDAKALSDRVEEVLDQLESLQ+KLES VEEME++KQ L NGN+ R+EHKKY
Sbjct: 61  TTKQVYEASFDAKALSDRVEEVLDQLESLQDKLESAVEEMERDKQALSNGNMRRIEHKKY 120

Query: 121 LEEEVIKPLYNAHIALRQIRLPKVEKVDQNSTMKEEPLINNFVVEEIRKYISPKKSRIGK 180
           LE EVIKPLYNAHIALRQIRLPKVEKV +N ++KEEPLINNFVVEEIRKYI+PKKSR GK
Sbjct: 121 LEMEVIKPLYNAHIALRQIRLPKVEKVKENLSVKEEPLINNFVVEEIRKYITPKKSRTGK 180

Query: 181 LNMYMTENIYNTIGHACVLHRKELEEYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCL 240
           +NMYMTE+IYNTIGHACVL RKELEEYM YDIGSYCKDDWN+ QKLMLNGCDPLPRRRCL
Sbjct: 181 VNMYMTESIYNTIGHACVLVRKELEEYMSYDIGSYCKDDWNVGQKLMLNGCDPLPRRRCL 240

Query: 241 ARASKVYQKPYPVNESLWKSPDDRNVRWSNYQCRNFSCLSSKNPRRGYNKCSGCFEMEKE 300
            RASKVYQKPY VNESLWK+PDDRNVRWSNYQCRNFSCLSSKNP+RGYNKCSGCFEMEKE
Sbjct: 241 TRASKVYQKPYAVNESLWKTPDDRNVRWSNYQCRNFSCLSSKNPKRGYNKCSGCFEMEKE 300

Query: 301 KLKWVTNSSLPIDFAISDVLTIKPGEIRIGLDFGIGTGSFAARMREQNVTIVTTALNLGA 360
           K+KWV NSS+ +DF ISDVL +K GEIR+GLDFGI TGSF ARMRE+NVTIVTTALNLGA
Sbjct: 301 KVKWVRNSSVAVDFRISDVLGMKAGEIRVGLDFGISTGSFGARMREENVTIVTTALNLGA 360

Query: 361 PFNEFIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRP 420
           PFNEFIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGW+DMLLLDFILFDWDRVLRP
Sbjct: 361 PFNEFIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWVDMLLLDFILFDWDRVLRP 420

Query: 421 GGLLWIDRFFCDRKDLDDYMYMFLQFRYKKHKWAIAPKSKNEVYLSALLEKPPRAI 472
           GGLLWIDRFFCDRKDLDDY+YMFLQFRYKKHKWAIAPKSKNEVYLSA+LEKPPRAI
Sbjct: 421 GGLLWIDRFFCDRKDLDDYIYMFLQFRYKKHKWAIAPKSKNEVYLSAVLEKPPRAI 476

BLAST of Spg028107 vs. TAIR 10
Match: AT3G05390.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: mitochondrion; EXPRESSED IN: 15 plant structures; EXPRESSED DURING: 7 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF248, methyltransferase putative (InterPro:IPR004159); BEST Arabidopsis thaliana protein match is: S-adenosyl-L-methionine-dependent methyltransferases superfamily protein (TAIR:AT4G01240.1); Has 507 Blast hits to 498 proteins in 33 species: Archae - 4; Bacteria - 8; Metazoa - 0; Fungi - 0; Plants - 493; Viruses - 0; Other Eukaryotes - 2 (source: NCBI BLink). )

HSP 1 Score: 704.9 bits (1818), Expect = 4.3e-203
Identity = 335/464 (72.20%), Postives = 396/464 (85.34%), Query Frame = 0

Query: 8   KWRLGRTMGWLQILLGGLVIIMTTLSLFKFYSAGFIFHKEDVCRYFYTTTRNVYEASFDA 67
           +WRLGR M  LQ++LG LVI ++   L++F+S G+    ED C   Y T + V    FD 
Sbjct: 8   RWRLGRLMAGLQVILGLLVITVSLSCLYRFHSVGYFLQNEDTCNNIY-TIKEVSSEGFDL 67

Query: 68  KALSDRVEEVLDQLESLQEKLESTVEEMEKNKQVLRNGNITRLEHKKYLEEEVIKPLYNA 127
           KAL DRV+EVL+++++L EKLE TV++MEK+K      + ++ E KK+LE+EV+KP Y A
Sbjct: 68  KALHDRVDEVLEKMDNLYEKLEKTVKDMEKSK------DGSKKEMKKFLEDEVMKPFYYA 127

Query: 128 HIALRQIRLPKVEKVDQNSTMKEEPLINNFVVEEIRKYISPKKSRIGKLNMYMTENIYNT 187
           HI LRQIRLPK E + +NST KEEPLIN F++EEIR+YI+PK++R+GK+NM+ TE +YNT
Sbjct: 128 HIGLRQIRLPKPEGI-RNSTEKEEPLINKFLIEEIRQYITPKENRVGKINMFGTERVYNT 187

Query: 188 IGHACVLHRKELEEYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLARASKVYQKPYP 247
           IGHAC L + ELE+YMDYD+G+YC DDWNLAQKLMLNGCDPLPRRRCL RAS  YQKPYP
Sbjct: 188 IGHACALMKMELEKYMDYDVGAYCDDDWNLAQKLMLNGCDPLPRRRCLTRASMTYQKPYP 247

Query: 248 VNESLWKSPDDRNVRWSNYQCRNFSCLSSKNPRRGYNKCSGCFEMEKEKLKWVTNSSLPI 307
           +NESLWK PDDRNVRW NYQCRNF+CLSSKNP+RGY KCSGCFEMEKEK KWV NSSL +
Sbjct: 248 INESLWKLPDDRNVRWGNYQCRNFACLSSKNPKRGYTKCSGCFEMEKEKDKWVKNSSLLV 307

Query: 308 DFAISDVLTIKPGEIRIGLDFGIGTGSFAARMREQNVTIVTTALNLGAPFNEFIALRGLI 367
           DF I DVL +KP EIRIGLD+G+GTG+FAARMRE+NVTIVTTALNLGAPFNE IALRGLI
Sbjct: 308 DFMIEDVLRVKPSEIRIGLDYGVGTGTFAARMREKNVTIVTTALNLGAPFNEMIALRGLI 367

Query: 368 PLYVTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGLLWIDRFFCD 427
           PLY++LNQRLP FDNTMD+IHTTG MDGW+D+LL+DF+L+DWDRVLRPGGLLWIDRFFC 
Sbjct: 368 PLYISLNQRLPFFDNTMDMIHTTGLMDGWIDLLLMDFVLYDWDRVLRPGGLLWIDRFFCK 427

Query: 428 RKDLDDYMYMFLQFRYKKHKWAIAPKSKNEVYLSALLEKPPRAI 472
           +KDLDDYMYMFLQFRYKKHKWAI+PKSK+EVYLSALLEKPPRAI
Sbjct: 428 KKDLDDYMYMFLQFRYKKHKWAISPKSKDEVYLSALLEKPPRAI 463

BLAST of Spg028107 vs. TAIR 10
Match: AT4G01240.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 433.0 bits (1112), Expect = 3.2e-121
Identity = 197/325 (60.62%), Postives = 250/325 (76.92%), Query Frame = 0

Query: 156 NFVVEEIRKYISPKKSRIGKLNMYMTENIYNTIGHACVLHRKELEEYMDYDIGSYCKDDW 215
           +F+ EEI+KYI  K +R+GK N       + +IGHAC   +K+LEEYMDYD+G  C DDW
Sbjct: 332 HFMREEIKKYIKIKPNRLGKQNFMGANGTFTSIGHACFAMKKDLEEYMDYDVGEICNDDW 391

Query: 216 NLAQKLMLNGCDPLPRRRCLARASKVYQKPYPVNESLWKSPDDRNVRWSNYQCRNFSCLS 275
            LAQKLM++GCDPLPRRRC +R  ++Y KP+P+NESLWK PD+RNVRW  Y+C+NF+CL+
Sbjct: 392 RLAQKLMVHGCDPLPRRRCFSRGPQLYHKPFPINESLWKLPDNRNVRWGQYKCKNFACLA 451

Query: 276 SK-NPRRGYNKCSGCFEM-EKEKLKWVTNSSL------PIDFAISDVLTIKPGEIRIGLD 335
           S    R+G+ KC+ CF +   E  +W+    +        DF+I++VL IKPGEIRIGLD
Sbjct: 452 SNTTARKGFFKCTDCFNLTHHESPRWLNRGEIDPETNQTADFSIAEVLEIKPGEIRIGLD 511

Query: 336 FGIGTGSFAARMREQNVTIVTTALNLGAPFNEFIALRGLIPLYVTLNQRLPLFDNTMDLI 395
           F IGTG+FAARMREQNVTIV+  +NLGAPFNE IALRGL+PLY+T+NQRLP FD+T+D+I
Sbjct: 512 FSIGTGTFAARMREQNVTIVSATINLGAPFNEMIALRGLVPLYLTVNQRLPFFDSTLDMI 571

Query: 396 HTTGFMDGWLDMLLLDFILFDWDRVLRPGGLLWIDRFFCDRKDLDDYMYMFLQFRYKKHK 455
           HTT F+DGW+D++LLDF+LFDWDRVLRPGGLLWID FFC ++D+ DYM  F   RY+KHK
Sbjct: 572 HTTRFLDGWIDLILLDFVLFDWDRVLRPGGLLWIDGFFCLKEDVSDYMEAFKALRYRKHK 631

Query: 456 WAIAP---KSKNEVYLSALLEKPPR 470
           W + P   K   EV+ SA+LEKPPR
Sbjct: 632 WVVVPKKDKDDKEVFFSAVLEKPPR 656

BLAST of Spg028107 vs. TAIR 10
Match: AT3G27230.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 257.3 bits (656), Expect = 2.4e-68
Identity = 133/314 (42.36%), Postives = 194/314 (61.78%), Query Frame = 0

Query: 158 VVEEIRKYISPKKSRIGKLNMYMTENIYNTIGHACVLHRKELEEYMDYDIGSYCKDDWNL 217
           +V E++ ++   +  +GK +      + +++GH+C      L +YM Y +   C DDW+L
Sbjct: 102 IVPELKLFLQRHQLPLGKDSRTGITEMVSSVGHSCGKSTDLLSQYMSYKVFDRCPDDWSL 161

Query: 218 AQKLMLNGCDPLPRRRCLARASKVYQKPYPVNESLWKSPDDRNVRWSNYQCRNFSCLSSK 277
            QKL+L  C+PLPRRRCLA+  +  Q      +SLW+S  +++V WS   C++F CL  K
Sbjct: 162 GQKLILRACEPLPRRRCLAKTVQ-KQDLSKSPDSLWRSVSNKSVNWSGLGCKSFDCLKGK 221

Query: 278 NPRRGYNKCSGCFEMEKEKLKWVTNSSLPIDFAISDVLTIKPGEIRIGLDFGIGTGSFAA 337
              +   +C GCF++  EK ++V       DF I DVL +  G+IRIG D   G+G+FAA
Sbjct: 222 KLSK---ECVGCFDLGVEKDRFVKVKG-KNDFLIDDVLGLGSGKIRIGFDISGGSGTFAA 281

Query: 338 RMREQNVTIVTTALNLGAPFNEFIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWL 397
           RM E+NVT++T  LN GAPF+EFIA RGL PL+++L+ R P  DN  DLIH +  +D   
Sbjct: 282 RMAEKNVTVITNTLNNGAPFSEFIAARGLFPLFLSLDHRFPFLDNVFDLIHASSGLDVEG 341

Query: 398 DMLLLDFILFDWDRVLRPGGLLWIDRFFC-DRKDLDDYMYMFLQFRYKKHKWAIAPKSKN 457
               L+F++FD DRVL+P GL W+D F+C + +   +   M  +F YKK KW I  K+  
Sbjct: 342 KAEKLEFLMFDLDRVLKPRGLFWLDNFYCANDEKKKELTRMIERFGYKKLKWVIGEKADA 401

Query: 458 EVYLSALLEKPPRA 471
           +VYLSA+L+KP RA
Sbjct: 402 QVYLSAVLQKPVRA 410

BLAST of Spg028107 vs. TAIR 10
Match: AT5G40830.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 253.8 bits (647), Expect = 2.6e-67
Identity = 137/361 (37.95%), Postives = 213/361 (59.00%), Query Frame = 0

Query: 120 VIKPLYNAHIALRQIRLPKVEKVDQNSTMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY 179
           +++ + ++H  L Q+    +     + + +E P       +E++ ++   +  +GK +  
Sbjct: 71  ILREIDSSHHTLTQMEKQIIGYESLDLSQQEVP-------QELKLFLQQHQLPLGKDSRT 130

Query: 180 MTENIYNTIGHACVLHRKELEEYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLARAS 239
               +  ++GH+C +    L +YM Y++   C DDW+LAQKL+L  C+PLPRRRCLA   
Sbjct: 131 GITQMVASVGHSCEMSLDLLSQYMSYNVFEKCPDDWSLAQKLILRACEPLPRRRCLA--- 190

Query: 240 KVYQKP----YPVNESLWKSPDDRNVRWSNYQCRNFSCLSSKNPRRGYNKCSGCFEM--- 299
           K   KP    +P  +SLW+   + +V WS   C++F CL  K   R    C GCF++   
Sbjct: 191 KTVHKPGLALFP--DSLWRPVGNSSVNWSGLGCKSFECLKGKKLSR---DCVGCFDLATS 250

Query: 300 -EKEKLKWVTNSSLPIDFAISDVLTIKPGEIRIGLDFGIGTGSFAARMREQNVTIVTTAL 359
            EK++   V   +   DF I DVL +  G+IRIG D   G+G+FAARM E+NV I++  L
Sbjct: 251 HEKDRFVKVNGKT---DFLIDDVLDLGDGKIRIGFDISSGSGTFAARMAEKNVNIISNTL 310

Query: 360 NLGAPFNEFIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWLDML--LLDFILFDW 419
           N+ APF+EFIA RG+ PL+++L+QRLP +DN  DLIH +  +D  +      L+F++FD 
Sbjct: 311 NIDAPFSEFIAARGIFPLFMSLDQRLPFYDNVFDLIHASNGLDLAVSNKPEKLEFLMFDL 370

Query: 420 DRVLRPGGLLWIDRFFCDRKDLDDYMYMFLQ-FRYKKHKWAIAPKSKNEVYLSALLEKPP 470
           DR+L+PGGL W+D F+C   +    +   ++ F YKK KW +  K+  EV+LSA+L+KP 
Sbjct: 371 DRILKPGGLFWLDNFYCGNDEKKRVLTRLIERFGYKKLKWVVGEKTDAEVFLSAVLQKPA 413

BLAST of Spg028107 vs. TAIR 10
Match: AT5G40830.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 253.8 bits (647), Expect = 2.6e-67
Identity = 137/361 (37.95%), Postives = 213/361 (59.00%), Query Frame = 0

Query: 120 VIKPLYNAHIALRQIRLPKVEKVDQNSTMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY 179
           +++ + ++H  L Q+    +     + + +E P       +E++ ++   +  +GK +  
Sbjct: 71  ILREIDSSHHTLTQMEKQIIGYESLDLSQQEVP-------QELKLFLQQHQLPLGKDSRT 130

Query: 180 MTENIYNTIGHACVLHRKELEEYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLARAS 239
               +  ++GH+C +    L +YM Y++   C DDW+LAQKL+L  C+PLPRRRCLA   
Sbjct: 131 GITQMVASVGHSCEMSLDLLSQYMSYNVFEKCPDDWSLAQKLILRACEPLPRRRCLA--- 190

Query: 240 KVYQKP----YPVNESLWKSPDDRNVRWSNYQCRNFSCLSSKNPRRGYNKCSGCFEM--- 299
           K   KP    +P  +SLW+   + +V WS   C++F CL  K   R    C GCF++   
Sbjct: 191 KTVHKPGLALFP--DSLWRPVGNSSVNWSGLGCKSFECLKGKKLSR---DCVGCFDLATS 250

Query: 300 -EKEKLKWVTNSSLPIDFAISDVLTIKPGEIRIGLDFGIGTGSFAARMREQNVTIVTTAL 359
            EK++   V   +   DF I DVL +  G+IRIG D   G+G+FAARM E+NV I++  L
Sbjct: 251 HEKDRFVKVNGKT---DFLIDDVLDLGDGKIRIGFDISSGSGTFAARMAEKNVNIISNTL 310

Query: 360 NLGAPFNEFIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWLDML--LLDFILFDW 419
           N+ APF+EFIA RG+ PL+++L+QRLP +DN  DLIH +  +D  +      L+F++FD 
Sbjct: 311 NIDAPFSEFIAARGIFPLFMSLDQRLPFYDNVFDLIHASNGLDLAVSNKPEKLEFLMFDL 370

Query: 420 DRVLRPGGLLWIDRFFCDRKDLDDYMYMFLQ-FRYKKHKWAIAPKSKNEVYLSALLEKPP 470
           DR+L+PGGL W+D F+C   +    +   ++ F YKK KW +  K+  EV+LSA+L+KP 
Sbjct: 371 DRILKPGGLFWLDNFYCGNDEKKRVLTRLIERFGYKKLKWVVGEKTDAEVFLSAVLQKPA 413

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038880508.11.4e-25694.02uncharacterized protein LOC120072172 [Benincasa hispida][more]
XP_022146046.16.0e-25592.37uncharacterized protein LOC111015349 [Momordica charantia][more]
XP_011659893.12.8e-25291.75uncharacterized protein LOC101207467 [Cucumis sativus] >KAE8653367.1 hypothetica... [more]
XP_008450643.18.1e-25291.75PREDICTED: uncharacterized protein LOC103492170 [Cucumis melo] >KAA0050891.1 put... [more]
XP_022969573.13.8e-24989.22uncharacterized protein LOC111468555 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q8RWB71.0e-5542.51Probable methyltransferase At1g29790 OS=Arabidopsis thaliana OX=3702 GN=CLSC5 PE... [more]
Match NameE-valueIdentityDescription
A0A6J1CY712.9e-25592.37uncharacterized protein LOC111015349 OS=Momordica charantia OX=3673 GN=LOC111015... [more]
A0A5D3CEF43.9e-25291.75Putative Methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A1S3BQC03.9e-25291.75uncharacterized protein LOC103492170 OS=Cucumis melo OX=3656 GN=LOC103492170 PE=... [more]
A0A6J1I1D31.8e-24989.22uncharacterized protein LOC111468555 OS=Cucurbita maxima OX=3661 GN=LOC111468555... [more]
A0A6J1EZZ01.3e-24788.66uncharacterized protein LOC111440913 OS=Cucurbita moschata OX=3662 GN=LOC1114409... [more]
Match NameE-valueIdentityDescription
AT3G05390.14.3e-20372.20FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
AT4G01240.13.2e-12160.62S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT3G27230.12.4e-6842.36S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G40830.12.6e-6737.95S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G40830.22.6e-6737.95S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 67..105
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 291..465
e-value: 1.0E-7
score: 33.4
NoneNo IPR availablePANTHERPTHR44067S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASE SUPERFAMILY PROTEIN-RELATEDcoord: 8..470
NoneNo IPR availablePANTHERPTHR44067:SF9F22F7.17 PROTEINcoord: 8..470
IPR004159Putative S-adenosyl-L-methionine-dependent methyltransferasePFAMPF03141Methyltransf_29coord: 315..421
e-value: 1.3E-6
score: 27.3
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 316..451

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg028107.1Spg028107.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0043231 intracellular membrane-bounded organelle
molecular_function GO:0008168 methyltransferase activity