Spg028099 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg028099 vs. NCBI nr
Match: KAA0036941.1 (girdin-like [Cucumis melo var. makuwa])

HSP 1 Score: 316.2 bits (809), Expect = 8.6e-82
Identity = 230/666 (34.53%), Postives = 337/666 (50.60%), Query Frame = 0

Query: 12  SSSSEYDELGTVLQWAEQTQLKHGDSL-PYKSLVSSSFSG---QVQVISNELGELKAIWE 71
           S  S++DE   VL+WAE+ Q K GD+L P +  + S   G   ++  I      L+AI  
Sbjct: 9   SFESKFDEPSDVLKWAEEMQQKFGDALTPQRRFMFSKKYGHIAELMYIPVNYFALRAI-I 68

Query: 72  EFWDPAYKCFAFQDFDLVPTIEEYHTMLNIEEKGGDMIYCFNPQLTAKRTLSKFL-AVHL 131
            F DPAY CF F   +L+PTIEEY  ML++ EK  +++Y FNP+ T KRTLSKFL  VH 
Sbjct: 69  NFGDPAYGCFTFGSCNLLPTIEEYQAMLSMPEKEREIVYFFNPKQTTKRTLSKFLETVHA 128

Query: 132 KEVQKNVKVKGVEESISADYLTELARKHINEEKGLVLLALCIYGMVLYPRVKRYVDDNVL 191
            E+QK +KVKG EE++  DYL ++ + +I+E+KGL LLALCIYG V++P+ + YVD  V+
Sbjct: 129 TEIQKYIKVKGGEENVPFDYLIKMTQTYIDEDKGLTLLALCIYGAVIFPKAEGYVDRKVI 188

Query: 192 KLFFSIEQGVDPAIPLLAETVRALNYCKSSKGGKLR-------IW-HG----PME----- 251
           KLFF +E+GV+P IP+LAET R+LNYC++   GKL        IW H     P E     
Sbjct: 189 KLFFQMERGVNPIIPILAETFRSLNYCRNKGEGKLNCCVPLLYIWIHSHIKFPAEFRCPR 248

Query: 252 LKFSEE-RTMGVLLFEFQIRACD-----------------LEDTLDAP-----KSLNLQM 311
           L FS     M   + EF +   D                  E+ +        K++  + 
Sbjct: 249 LDFSSPWNLMRNTISEFGMAVWDPTYPRKEAWLSFFAKLTSENVIWKAQWMPLKAVIYRC 308

Query: 312 RELPHSPSSRPLG---MRPLCSVVGLAAKL------------------DSNEKKRQVLTS 371
            +    P   P G     PL  +  +  K                   D   KKRQ + +
Sbjct: 309 GDFHSVPLLGPWGGVNYTPLLVLRQVWLKQFIPPTHNLQESDFSYDPEDCQGKKRQAVCA 368

Query: 372 WTTVRRINGNSHSEGVTPEYLQWRIKRRK-IPITTRDNVGESSNRAVDKPNQLATERKEL 431
           W ++R+I    H EGVT  Y  W+  RRK I   +R+ V      + ++PNQ   +  EL
Sbjct: 369 WKSIRKIKDKGHYEGVTSGYEAWQANRRKNIIDISREVVESGKETSFEQPNQWIEKSIEL 428

Query: 432 VGRNQTLKLENEKLQQEVKCLMNQVTQASGKLEEVERVSKNRAKRERDYDILDEENRRLI 491
             +N+ L+ ENEKL++E    M+  T    +LE+ +   KN+ K E+D + LDEE RR+ 
Sbjct: 429 EEKNRLLEQENEKLRKETSQWMDHATYLQNELEKTKSFLKNQDKLEKDLETLDEEMRRMN 488

Query: 492 KENHALRNENTALRRATRSQEDRVKDLSRAKETLLELVAELNEIINKQKTQFIEFEEANT 551
           K N +L+NE T L+                                              
Sbjct: 489 KANRSLKNEKTTLQATV------------------------------------------- 548

Query: 552 ALRRTLDNLRVNMHAQSEESKAFKSYSSSLERQLQAFQRASEQLSLERGQLEEKYSSLRG 611
                   L + M  +SEE +  K+Y+ SL  QL A Q +S++++ E   L   Y  ++ 
Sbjct: 549 -------GLHLKMAERSEEYEILKNYADSLHYQLTALQNSSKRITQEYESLNTDYVQMKV 608

BLAST of Spg028099 vs. NCBI nr
Match: KAA0036949.1 (girdin-like [Cucumis melo var. makuwa])

HSP 1 Score: 309.7 bits (792), Expect = 8.1e-80
Identity = 206/589 (34.97%), Postives = 317/589 (53.82%), Query Frame = 0

Query: 69  FWDPAYKCFAFQDFDLVPTIEEYHTMLNIEEKGGDMIYCFNPQLTAKRTLSKFL-AVHLK 128
           F DPAY CF F   DL+PTIEEY  ML++ +K  +++Y FNP+ T KRTLSKFL  VH  
Sbjct: 40  FGDPAYGCFTFGSCDLLPTIEEYQAMLSMPQKEREIVYFFNPKQTTKRTLSKFLETVHAT 99

Query: 129 EVQKNVKVKGVEESISADYLTELARKHINEEKGLVLLALCIYGMVLYPRVKRYVDDNVLK 188
           E+QK +K KG EE++  DYL ++ + +I+E+KGL LLALCIYG V++P+ + YVD  V+K
Sbjct: 100 EIQKYIKAKGGEENVPFDYLIKMTQTYIDEDKGLTLLALCIYGAVIFPKAEGYVDRKVIK 159

Query: 189 LFFSIEQGVDPAIPLLAETVRALNYCKSSKGGKLRIWHGPMELKFSEERTMGVLLFEFQI 248
           LFF +E+GV+P IP+LAET R+LNYC++   GKL                 G L     +
Sbjct: 160 LFFQMERGVNPIIPILAETFRSLNYCRNKGEGKLNCC------------VRGKLNCCVPL 219

Query: 249 RACDLEDTLDAPKSLNLQMRELPHSPSSRPLG-MRPLCSVVGLAAKLDSNEKKRQVLT-- 308
               +   +  P          P    S P   MR   S  G+A    +  +K   L+  
Sbjct: 220 LYIWIHSHIKFPAEF-----RCPRLDFSSPWNLMRNTISEFGMAVWDPTYPRKEAWLSFF 279

Query: 309 ------------SWTTVRRI---NGNSHS---------EGVTPEYLQWRI-KRRKIPIT- 368
                        W  ++ +    G+ H+            TP  +  ++  ++ IP T 
Sbjct: 280 AKLTSENVIWKAQWMPLKAVIYRCGDFHNVPLLGPWGGVNCTPLLVLRQVWLKQFIPPTH 339

Query: 369 -----------------TRDNVGESSNRAVDKPNQLATERKELVGRNQTLKLENEKLQQE 428
                               ++      + ++PNQ   +  EL  +N+ L+ ENEKL++E
Sbjct: 340 NLQESDFSYDPEDCQGKKTSSIERGKETSFEQPNQWIEKSIELEEKNRLLEQENEKLRKE 399

Query: 429 VKCLMNQVTQASGKLEEVERVSKNRAKRERDYDILDEENRRLIKENHALRNENTALRRAT 488
               M+  T    KLE+ +   KN+ K E+D + LD+E RR+ K N +L+NE T  +   
Sbjct: 400 TSQWMDHATYLQNKLEKTKSFLKNQDKLEKDLETLDKEMRRMNKANRSLKNEKTTFQATM 459

Query: 489 RSQEDRVKDLSRAKETLLELVAELNEIINKQKTQFIEFEEANTALRRTLDNLRVNMHAQS 548
            SQ++ +KDL   KE  L+LV +LN  I K++T+ ++ E  N +LR+T+D+L + M   S
Sbjct: 460 GSQDEYIKDLENGKEYFLKLVNDLNTSIRKRETKIMDLEAQNHSLRQTVDSLHLKMVEHS 519

Query: 549 EESKAFKSYSSSLERQLQAFQRASEQLSLERGQLEEKYSSLRGDYAIMREDMQIILGKVN 608
           EE +  K+Y+ SL  QL A Q +S++++ E   L   Y  ++ DY +   D Q+++ +V+
Sbjct: 520 EEYEILKNYADSLHYQLTALQNSSKRITQEYESLNTDYVQMKVDYDMQTRDFQVLVERVD 579

Query: 609 QTMNTIKIMARRARGFAEWARDLRENTSPMASNAEELFEFLGMIRRDLG 611
           QT+  ++++++RA  FAE A DLR N   M  +A++L  FL MI R+LG
Sbjct: 580 QTIEFLRMVSKRANDFAESAADLRVNFFSMQPHADDLNRFLKMICRELG 611

BLAST of Spg028099 vs. NCBI nr
Match: KAA0066094.1 (girdin-like [Cucumis melo var. makuwa])

HSP 1 Score: 271.2 bits (692), Expect = 3.2e-68
Identity = 185/533 (34.71%), Postives = 268/533 (50.28%), Query Frame = 0

Query: 12  SSSSEYDELGTVLQWAEQTQLKHGDSLPYKSLVSSSFSGQVQVISNELGELKAIWE---- 71
           S  S++DE   VL+WAE+ Q K GD +   S +S     Q+    N+L  LK IWE    
Sbjct: 9   SFESKFDEPSDVLKWAEEMQQKFGDGINNSSQISVLSRCQLSFTQNDLANLKMIWEALTP 68

Query: 72  ------------------------------EFWDPAYKCFAFQDFDLVPTIEEYHTMLNI 131
                                          FWDPAY CF F   DL+PTIEEY  ML++
Sbjct: 69  QRRFMFSKKYGHIAELMYIPVNYFALRAIINFWDPAYGCFTFGSCDLLPTIEEYQAMLSM 128

Query: 132 EEKGGDMIYCFNPQLTAKRTLSKFL-AVHLKEVQKNVKVKGVEESISADYLTELARKHIN 191
             K  +++Y FNP+ T KRTLSKFL  VH  ++QK +KVKG EE++  DYL ++ + +++
Sbjct: 129 PGKEREIVYFFNPKKTTKRTLSKFLETVHAAKIQKYIKVKGGEENVPFDYLIKMTQTYVD 188

Query: 192 EEKGLVLLALCIYGMVLYPRVKRYVDDNVLKLFFSIEQGVDPAIPLLAETVRALNYCKSS 251
           E+KGL LLALCIYG V++P+ + YVD   +        G   ++PLL      +NY    
Sbjct: 189 EDKGLTLLALCIYGAVIFPKAEGYVDGKAV----IYRCGDFHSVPLLGPW-GGVNYT--- 248

Query: 252 KGGKLRIWHGPMELKFSEERTMGVLLFEFQIRACDLEDTLDAPKSLNLQMRELPHSPSSR 311
               L + H              V L +F             P + NLQ  +  + P   
Sbjct: 249 ---PLLVLH-------------QVWLKQF------------IPPTHNLQEFDFSYDPE-- 308

Query: 312 PLGMRPLCSVVGLAAKLDSNEKKRQVLTSWTTVRRINGNSHSEGVTPEYLQWRIKRRK-I 371
                            D   KKRQ + +W ++R+I    H EGVT  Y  W+  RRK I
Sbjct: 309 -----------------DCQGKKRQAVCAWKSIRKIKDKGHYEGVTSGYEAWQANRRKNI 368

Query: 372 PITTRDNVGESSNRAVDKPNQLATERKELVGRNQTLKLENEKLQQEVKCLMNQVTQASGK 431
              +R+ V      + ++PNQ   +  EL  +N+ L+ ENEKL++E    M+  T    +
Sbjct: 369 IDISREVVERGKKTSFEQPNQWIEKSIELEEKNRLLEQENEKLRKETSQWMDHATYLQNE 428

Query: 432 LEEVERVSKNRAKRERDYDILDEENRRLIKENHALRNENTALRRATRSQEDRVKDLSRAK 491
           LE+ +   KN+ K E+D + LD+E RR+ K N +L+NE T L+    SQ++ +KDL   K
Sbjct: 429 LEKTKSFLKNQDKLEKDLETLDKEMRRMNKANRSLKNEKTTLQATMGSQDEYIKDLENGK 486

Query: 492 ETLLELVAELNEIINKQKTQFIEFEEANTALRRTLDNLRVNMHAQSEESKAFK 509
           E  LE V +LN  I K++TQ ++ E  N +LR+T+D+L + M   SEE +  K
Sbjct: 489 EYFLERVNDLNTSIGKRETQIMDLEAQNHSLRQTVDSLHLKMAECSEEYEILK 486

BLAST of Spg028099 vs. NCBI nr
Match: KAA0036933.1 (uncharacterized protein E6C27_scaffold86G00060 [Cucumis melo var. makuwa])

HSP 1 Score: 264.2 bits (674), Expect = 3.9e-66
Identity = 220/688 (31.98%), Postives = 326/688 (47.38%), Query Frame = 0

Query: 69  FWDPAYKCFAFQDFDLVPTIEEYHTMLNIEEKGGDMIYCFNPQLTAKRTLSKFL-AVHLK 128
           F DPAY CF F   DL+PTIEEY  ML++ +K  +++Y FNP+ T KRTLSKFL  VH  
Sbjct: 40  FGDPAYGCFTFGSCDLLPTIEEYQAMLSMPQKEREIVYFFNPKQTTKRTLSKFLETVHAT 99

Query: 129 EVQKNVKVKGVEESISADYLTELARKHINEEKGLVLLALCIYGMVLYPRVKRYVDDNVLK 188
           E+QK +K KG EE++  DYL ++ + +I+E+KGL LLALCIYG V++P+ + YVD  V+K
Sbjct: 100 EIQKYIKAKGGEENVPFDYLIKMTQTYIDEDKGLTLLALCIYGAVIFPKAEGYVDRKVIK 159

Query: 189 LFFSIEQGVDPAIPLLAETVRALNYCKSSKGGKLR---------------IW-HG----P 248
           LFF +E+GV+P IP+LAET R+LNYC++   GKL                IW H     P
Sbjct: 160 LFFQMERGVNPIIPILAETFRSLNYCRNKGEGKLNCCVRGKLNCCVPLLYIWIHSHIKFP 219

Query: 249 ME-----LKFSEE-RTMGVLLFEFQIRACDLEDTLDAPKSLNLQMRELPHSPSSRPLGMR 308
            E     L FS     M   + EF +   D   T    ++      +L            
Sbjct: 220 AEFRCPRLDFSSPWNLMRNTISEFGMAVWD--PTYPRKEAWLSFFAKLTSENVIWKAQWM 279

Query: 309 PLCSVVGLAAKLDS----------NEKKRQVLTS-W-------TTVRRINGNSHSEGVTP 368
           PL +V+       S          N     VL   W       T   +I    H EGVT 
Sbjct: 280 PLKAVIYRCGDFHSVPLLGPWGGVNYTPLLVLRQVWLKQFIPPTHNLKIKDKGHYEGVTS 339

Query: 369 EYLQWRIKRRK-IPITTRDNVGESSNRAVDKPNQLATERKELVGRNQTLKLENEKLQQEV 428
            Y  W+  RRK I   +R+ V      + ++PNQ   +  EL  +N+ L+ ENEKL++E 
Sbjct: 340 GYEAWQANRRKNIIDISREVVERGKETSFEQPNQWIEKSIELEQKNRLLEQENEKLRKET 399

Query: 429 KCLMNQVTQASGKLEEVERVSKNRAKRERDYDILDEENRRLIKENHALRNENTALRRATR 488
              M+  T    +LE+ +   KN+ K E D + LD+E RR+ K N +++NE T L+    
Sbjct: 400 SQWMDHATYLQNELEKTKSFLKNQDKLETDLETLDKEMRRMNKANRSMKNEKTTLQATV- 459

Query: 489 SQEDRVKDLSRAKETLLELVAELNEIINKQKTQFIEFEEANTALRRTLDNLRVNMHAQSE 548
                                                             L + M  +SE
Sbjct: 460 -------------------------------------------------GLHLKMAERSE 519

Query: 549 ESKAFKSYSSSLERQLQAFQRASEQLSLERGQLEEKYSSLRGDYAIMREDMQIILGKVNQ 608
           E K  K+Y+  L  QL A Q +S++++ E   L   Y  ++ DY +   D Q+++ +V+Q
Sbjct: 520 EYKILKNYADFLHYQLTALQNSSKRITQEYESLNTDYVQMKVDYDLHTRDFQVLVERVDQ 579

Query: 609 TMNTIKIMARRARGFAEWARDLRENTSPMASNAEELFEFLGMIRRDLGRR--TRIMEKKG 668
           T+  ++++++RA GFAEWA      T                IR     R  ++IME+K 
Sbjct: 580 TIEFLRMVSKRANGFAEWAGKYSSFTP---------------IRHPYNTRYKSQIMEEKD 639

Query: 669 -EQEKTKRDIEEIREKVDAIIAALE--KGNMIADTTTPDTPIGNPQAGLPFPP-----KI 700
            + +K +++I  + E+V  I+  L   KG    DTT    PI +    + +PP      I
Sbjct: 640 KDMDKMRQEINNLGEQVSKILELLSMGKGKAAVDTTQSSNPIQDTDDPI-YPPGFTPYHI 659

BLAST of Spg028099 vs. NCBI nr
Match: KAA0036933.1 (uncharacterized protein E6C27_scaffold86G00060 [Cucumis melo var. makuwa])

HSP 1 Score: 63.2 bits (152), Expect = 1.3e-05
Identity = 37/84 (44.05%), Postives = 50/84 (59.52%), Query Frame = 0

Query: 767  ISGTGGVTRSGKCYTPDQLLNLSKNTKRFGLGYKPDKADMIRVQKREKERRLARFESREP 826
            I G GG + +    T   LL +  N  RFGLGYKP   D IR+Q+++K++RL + E RE 
Sbjct: 1372 IMGGGGYSLNKNLET---LLKIPSNDGRFGLGYKPSIYDKIRLQEKKKKKRLVKLEMREF 1431

Query: 827  EYGEKNIPHLYHSFESAGIIRPGD 851
            +   K IP LY +F+SAGI    D
Sbjct: 1432 DPSIKLIPELYDTFKSAGISYSSD 1452

HSP 2 Score: 224.2 bits (570), Expect = 4.5e-54
Identity = 168/498 (33.73%), Postives = 234/498 (46.99%), Query Frame = 0

Query: 12  SSSSEYDELGTVLQWAEQTQLKHGDSLPYKSLVSSSFSGQVQVISNELGELKAIWE---- 71
           S  S++DE   VL+WAE+ Q K GD +   S +S     Q+    N+L  LK IWE    
Sbjct: 9   SFESKFDEPSDVLKWAEEMQQKFGDDINNSSQISVLSRCQLSFTQNDLANLKMIWEALTP 68

Query: 72  ------------------------------EFWDPAYKCFAFQDFDLVPTIEEYHTMLNI 131
                                          FWDPAY CF F   DL+PTIEEY  ML++
Sbjct: 69  QRRFMFSKKYGHIVELMYIPVNYFALRAIINFWDPAYGCFTFGSCDLLPTIEEYQAMLSM 128

Query: 132 EEKGGDMIYCFNPQLTAKRTLSKFL-AVHLKEVQKNVKVKGVEESISADYLTELARKHIN 191
            EK  +++Y FNP+ T KRTLSKFL  VH  E+QK +K+KG EE++  DYL ++ + +++
Sbjct: 129 PEKEREIVYFFNPKQTTKRTLSKFLETVHAAEIQKYIKIKGGEENVPFDYLIKMTQTYVD 188

Query: 192 EEKGLVLLALCIYGMVLYPRVKRYVDDNVLKLFFSIEQGVDPAIPLLAETVRALNYCKSS 251
           E+KGL LLALCIYG V++P+ + YVD  V+KLFF +E+GV+P IP+LAET R+LNYC++ 
Sbjct: 189 EDKGLTLLALCIYGAVIFPKAEGYVDGKVIKLFFEMERGVNPIIPILAETFRSLNYCRNK 248

Query: 252 KGGKLR-------IW-HGPMELKFSEERTMGVLLF------------EFQIRACD----- 311
             GKL        IW H    +KF  E     L F            EF +   D     
Sbjct: 249 GEGKLNCCLPLLYIWIHS--HIKFPAEFRCPRLDFSSPWNLIRNTISEFGMAVWDPTYPR 308

Query: 312 LEDTLD-----------------APKSLNLQMRELPHSPSSRPLG---MRPLCSVVGLAA 371
            E  L                    K++  + R+    P   P G     PL  +  +  
Sbjct: 309 KEAWLSFFAKLTSKNVVWKAQWMPLKAVIYRCRDFHSMPLLGPRGGVNYTPLLVLRQVWL 368

Query: 372 KL------------------DSNEKKRQVLTSWTTVRRINGNSHSEGVTPEYLQWRIKRR 410
           K                   D   KKRQ + +W ++R+I    H EGV   Y  W+  RR
Sbjct: 369 KQFIPPTHNLQESDFSYDPEDCQGKKRQAVCAWKSIRKIKDKGHYEGVISGYEAWQANRR 428

BLAST of Spg028099 vs. ExPASy TrEMBL
Match: A0A5A7T5S7 (Girdin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold86G00170 PE=4 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 4.2e-82
Identity = 230/666 (34.53%), Postives = 337/666 (50.60%), Query Frame = 0

Query: 12  SSSSEYDELGTVLQWAEQTQLKHGDSL-PYKSLVSSSFSG---QVQVISNELGELKAIWE 71
           S  S++DE   VL+WAE+ Q K GD+L P +  + S   G   ++  I      L+AI  
Sbjct: 9   SFESKFDEPSDVLKWAEEMQQKFGDALTPQRRFMFSKKYGHIAELMYIPVNYFALRAI-I 68

Query: 72  EFWDPAYKCFAFQDFDLVPTIEEYHTMLNIEEKGGDMIYCFNPQLTAKRTLSKFL-AVHL 131
            F DPAY CF F   +L+PTIEEY  ML++ EK  +++Y FNP+ T KRTLSKFL  VH 
Sbjct: 69  NFGDPAYGCFTFGSCNLLPTIEEYQAMLSMPEKEREIVYFFNPKQTTKRTLSKFLETVHA 128

Query: 132 KEVQKNVKVKGVEESISADYLTELARKHINEEKGLVLLALCIYGMVLYPRVKRYVDDNVL 191
            E+QK +KVKG EE++  DYL ++ + +I+E+KGL LLALCIYG V++P+ + YVD  V+
Sbjct: 129 TEIQKYIKVKGGEENVPFDYLIKMTQTYIDEDKGLTLLALCIYGAVIFPKAEGYVDRKVI 188

Query: 192 KLFFSIEQGVDPAIPLLAETVRALNYCKSSKGGKLR-------IW-HG----PME----- 251
           KLFF +E+GV+P IP+LAET R+LNYC++   GKL        IW H     P E     
Sbjct: 189 KLFFQMERGVNPIIPILAETFRSLNYCRNKGEGKLNCCVPLLYIWIHSHIKFPAEFRCPR 248

Query: 252 LKFSEE-RTMGVLLFEFQIRACD-----------------LEDTLDAP-----KSLNLQM 311
           L FS     M   + EF +   D                  E+ +        K++  + 
Sbjct: 249 LDFSSPWNLMRNTISEFGMAVWDPTYPRKEAWLSFFAKLTSENVIWKAQWMPLKAVIYRC 308

Query: 312 RELPHSPSSRPLG---MRPLCSVVGLAAKL------------------DSNEKKRQVLTS 371
            +    P   P G     PL  +  +  K                   D   KKRQ + +
Sbjct: 309 GDFHSVPLLGPWGGVNYTPLLVLRQVWLKQFIPPTHNLQESDFSYDPEDCQGKKRQAVCA 368

Query: 372 WTTVRRINGNSHSEGVTPEYLQWRIKRRK-IPITTRDNVGESSNRAVDKPNQLATERKEL 431
           W ++R+I    H EGVT  Y  W+  RRK I   +R+ V      + ++PNQ   +  EL
Sbjct: 369 WKSIRKIKDKGHYEGVTSGYEAWQANRRKNIIDISREVVESGKETSFEQPNQWIEKSIEL 428

Query: 432 VGRNQTLKLENEKLQQEVKCLMNQVTQASGKLEEVERVSKNRAKRERDYDILDEENRRLI 491
             +N+ L+ ENEKL++E    M+  T    +LE+ +   KN+ K E+D + LDEE RR+ 
Sbjct: 429 EEKNRLLEQENEKLRKETSQWMDHATYLQNELEKTKSFLKNQDKLEKDLETLDEEMRRMN 488

Query: 492 KENHALRNENTALRRATRSQEDRVKDLSRAKETLLELVAELNEIINKQKTQFIEFEEANT 551
           K N +L+NE T L+                                              
Sbjct: 489 KANRSLKNEKTTLQATV------------------------------------------- 548

Query: 552 ALRRTLDNLRVNMHAQSEESKAFKSYSSSLERQLQAFQRASEQLSLERGQLEEKYSSLRG 611
                   L + M  +SEE +  K+Y+ SL  QL A Q +S++++ E   L   Y  ++ 
Sbjct: 549 -------GLHLKMAERSEEYEILKNYADSLHYQLTALQNSSKRITQEYESLNTDYVQMKV 608

BLAST of Spg028099 vs. ExPASy TrEMBL
Match: A0A5A7T6E2 (Girdin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold86G00290 PE=4 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 3.9e-80
Identity = 206/589 (34.97%), Postives = 317/589 (53.82%), Query Frame = 0

Query: 69  FWDPAYKCFAFQDFDLVPTIEEYHTMLNIEEKGGDMIYCFNPQLTAKRTLSKFL-AVHLK 128
           F DPAY CF F   DL+PTIEEY  ML++ +K  +++Y FNP+ T KRTLSKFL  VH  
Sbjct: 40  FGDPAYGCFTFGSCDLLPTIEEYQAMLSMPQKEREIVYFFNPKQTTKRTLSKFLETVHAT 99

Query: 129 EVQKNVKVKGVEESISADYLTELARKHINEEKGLVLLALCIYGMVLYPRVKRYVDDNVLK 188
           E+QK +K KG EE++  DYL ++ + +I+E+KGL LLALCIYG V++P+ + YVD  V+K
Sbjct: 100 EIQKYIKAKGGEENVPFDYLIKMTQTYIDEDKGLTLLALCIYGAVIFPKAEGYVDRKVIK 159

Query: 189 LFFSIEQGVDPAIPLLAETVRALNYCKSSKGGKLRIWHGPMELKFSEERTMGVLLFEFQI 248
           LFF +E+GV+P IP+LAET R+LNYC++   GKL                 G L     +
Sbjct: 160 LFFQMERGVNPIIPILAETFRSLNYCRNKGEGKLNCC------------VRGKLNCCVPL 219

Query: 249 RACDLEDTLDAPKSLNLQMRELPHSPSSRPLG-MRPLCSVVGLAAKLDSNEKKRQVLT-- 308
               +   +  P          P    S P   MR   S  G+A    +  +K   L+  
Sbjct: 220 LYIWIHSHIKFPAEF-----RCPRLDFSSPWNLMRNTISEFGMAVWDPTYPRKEAWLSFF 279

Query: 309 ------------SWTTVRRI---NGNSHS---------EGVTPEYLQWRI-KRRKIPIT- 368
                        W  ++ +    G+ H+            TP  +  ++  ++ IP T 
Sbjct: 280 AKLTSENVIWKAQWMPLKAVIYRCGDFHNVPLLGPWGGVNCTPLLVLRQVWLKQFIPPTH 339

Query: 369 -----------------TRDNVGESSNRAVDKPNQLATERKELVGRNQTLKLENEKLQQE 428
                               ++      + ++PNQ   +  EL  +N+ L+ ENEKL++E
Sbjct: 340 NLQESDFSYDPEDCQGKKTSSIERGKETSFEQPNQWIEKSIELEEKNRLLEQENEKLRKE 399

Query: 429 VKCLMNQVTQASGKLEEVERVSKNRAKRERDYDILDEENRRLIKENHALRNENTALRRAT 488
               M+  T    KLE+ +   KN+ K E+D + LD+E RR+ K N +L+NE T  +   
Sbjct: 400 TSQWMDHATYLQNKLEKTKSFLKNQDKLEKDLETLDKEMRRMNKANRSLKNEKTTFQATM 459

Query: 489 RSQEDRVKDLSRAKETLLELVAELNEIINKQKTQFIEFEEANTALRRTLDNLRVNMHAQS 548
            SQ++ +KDL   KE  L+LV +LN  I K++T+ ++ E  N +LR+T+D+L + M   S
Sbjct: 460 GSQDEYIKDLENGKEYFLKLVNDLNTSIRKRETKIMDLEAQNHSLRQTVDSLHLKMVEHS 519

Query: 549 EESKAFKSYSSSLERQLQAFQRASEQLSLERGQLEEKYSSLRGDYAIMREDMQIILGKVN 608
           EE +  K+Y+ SL  QL A Q +S++++ E   L   Y  ++ DY +   D Q+++ +V+
Sbjct: 520 EEYEILKNYADSLHYQLTALQNSSKRITQEYESLNTDYVQMKVDYDMQTRDFQVLVERVD 579

Query: 609 QTMNTIKIMARRARGFAEWARDLRENTSPMASNAEELFEFLGMIRRDLG 611
           QT+  ++++++RA  FAE A DLR N   M  +A++L  FL MI R+LG
Sbjct: 580 QTIEFLRMVSKRANDFAESAADLRVNFFSMQPHADDLNRFLKMICRELG 611

BLAST of Spg028099 vs. ExPASy TrEMBL
Match: A0A5A7VFL0 (Girdin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold21G00850 PE=4 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 1.5e-68
Identity = 185/533 (34.71%), Postives = 268/533 (50.28%), Query Frame = 0

Query: 12  SSSSEYDELGTVLQWAEQTQLKHGDSLPYKSLVSSSFSGQVQVISNELGELKAIWE---- 71
           S  S++DE   VL+WAE+ Q K GD +   S +S     Q+    N+L  LK IWE    
Sbjct: 9   SFESKFDEPSDVLKWAEEMQQKFGDGINNSSQISVLSRCQLSFTQNDLANLKMIWEALTP 68

Query: 72  ------------------------------EFWDPAYKCFAFQDFDLVPTIEEYHTMLNI 131
                                          FWDPAY CF F   DL+PTIEEY  ML++
Sbjct: 69  QRRFMFSKKYGHIAELMYIPVNYFALRAIINFWDPAYGCFTFGSCDLLPTIEEYQAMLSM 128

Query: 132 EEKGGDMIYCFNPQLTAKRTLSKFL-AVHLKEVQKNVKVKGVEESISADYLTELARKHIN 191
             K  +++Y FNP+ T KRTLSKFL  VH  ++QK +KVKG EE++  DYL ++ + +++
Sbjct: 129 PGKEREIVYFFNPKKTTKRTLSKFLETVHAAKIQKYIKVKGGEENVPFDYLIKMTQTYVD 188

Query: 192 EEKGLVLLALCIYGMVLYPRVKRYVDDNVLKLFFSIEQGVDPAIPLLAETVRALNYCKSS 251
           E+KGL LLALCIYG V++P+ + YVD   +        G   ++PLL      +NY    
Sbjct: 189 EDKGLTLLALCIYGAVIFPKAEGYVDGKAV----IYRCGDFHSVPLLGPW-GGVNYT--- 248

Query: 252 KGGKLRIWHGPMELKFSEERTMGVLLFEFQIRACDLEDTLDAPKSLNLQMRELPHSPSSR 311
               L + H              V L +F             P + NLQ  +  + P   
Sbjct: 249 ---PLLVLH-------------QVWLKQF------------IPPTHNLQEFDFSYDPE-- 308

Query: 312 PLGMRPLCSVVGLAAKLDSNEKKRQVLTSWTTVRRINGNSHSEGVTPEYLQWRIKRRK-I 371
                            D   KKRQ + +W ++R+I    H EGVT  Y  W+  RRK I
Sbjct: 309 -----------------DCQGKKRQAVCAWKSIRKIKDKGHYEGVTSGYEAWQANRRKNI 368

Query: 372 PITTRDNVGESSNRAVDKPNQLATERKELVGRNQTLKLENEKLQQEVKCLMNQVTQASGK 431
              +R+ V      + ++PNQ   +  EL  +N+ L+ ENEKL++E    M+  T    +
Sbjct: 369 IDISREVVERGKKTSFEQPNQWIEKSIELEEKNRLLEQENEKLRKETSQWMDHATYLQNE 428

Query: 432 LEEVERVSKNRAKRERDYDILDEENRRLIKENHALRNENTALRRATRSQEDRVKDLSRAK 491
           LE+ +   KN+ K E+D + LD+E RR+ K N +L+NE T L+    SQ++ +KDL   K
Sbjct: 429 LEKTKSFLKNQDKLEKDLETLDKEMRRMNKANRSLKNEKTTLQATMGSQDEYIKDLENGK 486

Query: 492 ETLLELVAELNEIINKQKTQFIEFEEANTALRRTLDNLRVNMHAQSEESKAFK 509
           E  LE V +LN  I K++TQ ++ E  N +LR+T+D+L + M   SEE +  K
Sbjct: 489 EYFLERVNDLNTSIGKRETQIMDLEAQNHSLRQTVDSLHLKMAECSEEYEILK 486

BLAST of Spg028099 vs. ExPASy TrEMBL
Match: A0A5A7T1W2 (Retrotrans_gag domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold86G00060 PE=4 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 1.9e-66
Identity = 220/688 (31.98%), Postives = 326/688 (47.38%), Query Frame = 0

Query: 69  FWDPAYKCFAFQDFDLVPTIEEYHTMLNIEEKGGDMIYCFNPQLTAKRTLSKFL-AVHLK 128
           F DPAY CF F   DL+PTIEEY  ML++ +K  +++Y FNP+ T KRTLSKFL  VH  
Sbjct: 40  FGDPAYGCFTFGSCDLLPTIEEYQAMLSMPQKEREIVYFFNPKQTTKRTLSKFLETVHAT 99

Query: 129 EVQKNVKVKGVEESISADYLTELARKHINEEKGLVLLALCIYGMVLYPRVKRYVDDNVLK 188
           E+QK +K KG EE++  DYL ++ + +I+E+KGL LLALCIYG V++P+ + YVD  V+K
Sbjct: 100 EIQKYIKAKGGEENVPFDYLIKMTQTYIDEDKGLTLLALCIYGAVIFPKAEGYVDRKVIK 159

Query: 189 LFFSIEQGVDPAIPLLAETVRALNYCKSSKGGKLR---------------IW-HG----P 248
           LFF +E+GV+P IP+LAET R+LNYC++   GKL                IW H     P
Sbjct: 160 LFFQMERGVNPIIPILAETFRSLNYCRNKGEGKLNCCVRGKLNCCVPLLYIWIHSHIKFP 219

Query: 249 ME-----LKFSEE-RTMGVLLFEFQIRACDLEDTLDAPKSLNLQMRELPHSPSSRPLGMR 308
            E     L FS     M   + EF +   D   T    ++      +L            
Sbjct: 220 AEFRCPRLDFSSPWNLMRNTISEFGMAVWD--PTYPRKEAWLSFFAKLTSENVIWKAQWM 279

Query: 309 PLCSVVGLAAKLDS----------NEKKRQVLTS-W-------TTVRRINGNSHSEGVTP 368
           PL +V+       S          N     VL   W       T   +I    H EGVT 
Sbjct: 280 PLKAVIYRCGDFHSVPLLGPWGGVNYTPLLVLRQVWLKQFIPPTHNLKIKDKGHYEGVTS 339

Query: 369 EYLQWRIKRRK-IPITTRDNVGESSNRAVDKPNQLATERKELVGRNQTLKLENEKLQQEV 428
            Y  W+  RRK I   +R+ V      + ++PNQ   +  EL  +N+ L+ ENEKL++E 
Sbjct: 340 GYEAWQANRRKNIIDISREVVERGKETSFEQPNQWIEKSIELEQKNRLLEQENEKLRKET 399

Query: 429 KCLMNQVTQASGKLEEVERVSKNRAKRERDYDILDEENRRLIKENHALRNENTALRRATR 488
              M+  T    +LE+ +   KN+ K E D + LD+E RR+ K N +++NE T L+    
Sbjct: 400 SQWMDHATYLQNELEKTKSFLKNQDKLETDLETLDKEMRRMNKANRSMKNEKTTLQATV- 459

Query: 489 SQEDRVKDLSRAKETLLELVAELNEIINKQKTQFIEFEEANTALRRTLDNLRVNMHAQSE 548
                                                             L + M  +SE
Sbjct: 460 -------------------------------------------------GLHLKMAERSE 519

Query: 549 ESKAFKSYSSSLERQLQAFQRASEQLSLERGQLEEKYSSLRGDYAIMREDMQIILGKVNQ 608
           E K  K+Y+  L  QL A Q +S++++ E   L   Y  ++ DY +   D Q+++ +V+Q
Sbjct: 520 EYKILKNYADFLHYQLTALQNSSKRITQEYESLNTDYVQMKVDYDLHTRDFQVLVERVDQ 579

Query: 609 TMNTIKIMARRARGFAEWARDLRENTSPMASNAEELFEFLGMIRRDLGRR--TRIMEKKG 668
           T+  ++++++RA GFAEWA      T                IR     R  ++IME+K 
Sbjct: 580 TIEFLRMVSKRANGFAEWAGKYSSFTP---------------IRHPYNTRYKSQIMEEKD 639

Query: 669 -EQEKTKRDIEEIREKVDAIIAALE--KGNMIADTTTPDTPIGNPQAGLPFPP-----KI 700
            + +K +++I  + E+V  I+  L   KG    DTT    PI +    + +PP      I
Sbjct: 640 KDMDKMRQEINNLGEQVSKILELLSMGKGKAAVDTTQSSNPIQDTDDPI-YPPGFTPYHI 659

BLAST of Spg028099 vs. ExPASy TrEMBL
Match: A0A5A7T1W2 (Retrotrans_gag domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold86G00060 PE=4 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 6.4e-06
Identity = 37/84 (44.05%), Postives = 50/84 (59.52%), Query Frame = 0

Query: 767  ISGTGGVTRSGKCYTPDQLLNLSKNTKRFGLGYKPDKADMIRVQKREKERRLARFESREP 826
            I G GG + +    T   LL +  N  RFGLGYKP   D IR+Q+++K++RL + E RE 
Sbjct: 1372 IMGGGGYSLNKNLET---LLKIPSNDGRFGLGYKPSIYDKIRLQEKKKKKRLVKLEMREF 1431

Query: 827  EYGEKNIPHLYHSFESAGIIRPGD 851
            +   K IP LY +F+SAGI    D
Sbjct: 1432 DPSIKLIPELYDTFKSAGISYSSD 1452

HSP 2 Score: 224.2 bits (570), Expect = 2.2e-54
Identity = 168/498 (33.73%), Postives = 234/498 (46.99%), Query Frame = 0

Query: 12  SSSSEYDELGTVLQWAEQTQLKHGDSLPYKSLVSSSFSGQVQVISNELGELKAIWE---- 71
           S  S++DE   VL+WAE+ Q K GD +   S +S     Q+    N+L  LK IWE    
Sbjct: 9   SFESKFDEPSDVLKWAEEMQQKFGDDINNSSQISVLSRCQLSFTQNDLANLKMIWEALTP 68

Query: 72  ------------------------------EFWDPAYKCFAFQDFDLVPTIEEYHTMLNI 131
                                          FWDPAY CF F   DL+PTIEEY  ML++
Sbjct: 69  QRRFMFSKKYGHIVELMYIPVNYFALRAIINFWDPAYGCFTFGSCDLLPTIEEYQAMLSM 128

Query: 132 EEKGGDMIYCFNPQLTAKRTLSKFL-AVHLKEVQKNVKVKGVEESISADYLTELARKHIN 191
            EK  +++Y FNP+ T KRTLSKFL  VH  E+QK +K+KG EE++  DYL ++ + +++
Sbjct: 129 PEKEREIVYFFNPKQTTKRTLSKFLETVHAAEIQKYIKIKGGEENVPFDYLIKMTQTYVD 188

Query: 192 EEKGLVLLALCIYGMVLYPRVKRYVDDNVLKLFFSIEQGVDPAIPLLAETVRALNYCKSS 251
           E+KGL LLALCIYG V++P+ + YVD  V+KLFF +E+GV+P IP+LAET R+LNYC++ 
Sbjct: 189 EDKGLTLLALCIYGAVIFPKAEGYVDGKVIKLFFEMERGVNPIIPILAETFRSLNYCRNK 248

Query: 252 KGGKLR-------IW-HGPMELKFSEERTMGVLLF------------EFQIRACD----- 311
             GKL        IW H    +KF  E     L F            EF +   D     
Sbjct: 249 GEGKLNCCLPLLYIWIHS--HIKFPAEFRCPRLDFSSPWNLIRNTISEFGMAVWDPTYPR 308

Query: 312 LEDTLD-----------------APKSLNLQMRELPHSPSSRPLG---MRPLCSVVGLAA 371
            E  L                    K++  + R+    P   P G     PL  +  +  
Sbjct: 309 KEAWLSFFAKLTSKNVVWKAQWMPLKAVIYRCRDFHSMPLLGPRGGVNYTPLLVLRQVWL 368

Query: 372 KL------------------DSNEKKRQVLTSWTTVRRINGNSHSEGVTPEYLQWRIKRR 410
           K                   D   KKRQ + +W ++R+I    H EGV   Y  W+  RR
Sbjct: 369 KQFIPPTHNLQESDFSYDPEDCQGKKRQAVCAWKSIRKIKDKGHYEGVISGYEAWQANRR 428