Spg028063 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg028063
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	pectinesterase inhibitor-like
Location	scaffold2: 45997230 .. 45997802 (-)
RNA-Seq Expression	Spg028063
Synteny	Spg028063

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGTCCATTTCAGTTGCCATGGCTGTTGTTCTTTTGGCCTTTTCCCTCTGCCACGCCGAAAACACACAACAAAACCCAACCCTCGCAGGCAACGATCTCGTCGCCAAAACCTGCAGCAGCACCCCTTACAACGACCTCTGCAAAGCAATCTTACAATCATCGCCAAAAAGCAACGGAGCCGACCTCTACGGCCTCGCACACATCCTAATGGACATTGCAGCCGAGAATGTCACCAACATCTACGACAGCATAAACCAATTGCAAAACGACTCCAACATAGACAGTTTCATGGACGAGTGTTTGACGGATTGCCTGGAGAGCTACCAGGATGCAATCGACCAAATTGAAGACTCCGTCACTGCCTTGGAATTCAAAGCCTACAACGACGTTAAGACATGGGTTTCCGCTGCCATGAGCGATGCTGCTTCCTGTGACATGGGATTCAAAGACAGACCTGGCTATAAGAATCCAATTGCACCCATGACCACCACTTTTGATCAGATTTGCTCCATTATTTTGGCCATTACTCAACAGCTCTTAACTGGGGGGAATAGCAATGTCAGCAGCTAA

mRNA sequence

ATGAAGTCCATTTCAGTTGCCATGGCTGTTGTTCTTTTGGCCTTTTCCCTCTGCCACGCCGAAAACACACAACAAAACCCAACCCTCGCAGGCAACGATCTCGTCGCCAAAACCTGCAGCAGCACCCCTTACAACGACCTCTGCAAAGCAATCTTACAATCATCGCCAAAAAGCAACGGAGCCGACCTCTACGGCCTCGCACACATCCTAATGGACATTGCAGCCGAGAATGTCACCAACATCTACGACAGCATAAACCAATTGCAAAACGACTCCAACATAGACAGTTTCATGGACGAGTGTTTGACGGATTGCCTGGAGAGCTACCAGGATGCAATCGACCAAATTGAAGACTCCGTCACTGCCTTGGAATTCAAAGCCTACAACGACGTTAAGACATGGGTTTCCGCTGCCATGAGCGATGCTGCTTCCTGTGACATGGGATTCAAAGACAGACCTGGCTATAAGAATCCAATTGCACCCATGACCACCACTTTTGATCAGATTTGCTCCATTATTTTGGCCATTACTCAACAGCTCTTAACTGGGGGGAATAGCAATGTCAGCAGCTAA

Coding sequence (CDS)

ATGAAGTCCATTTCAGTTGCCATGGCTGTTGTTCTTTTGGCCTTTTCCCTCTGCCACGCCGAAAACACACAACAAAACCCAACCCTCGCAGGCAACGATCTCGTCGCCAAAACCTGCAGCAGCACCCCTTACAACGACCTCTGCAAAGCAATCTTACAATCATCGCCAAAAAGCAACGGAGCCGACCTCTACGGCCTCGCACACATCCTAATGGACATTGCAGCCGAGAATGTCACCAACATCTACGACAGCATAAACCAATTGCAAAACGACTCCAACATAGACAGTTTCATGGACGAGTGTTTGACGGATTGCCTGGAGAGCTACCAGGATGCAATCGACCAAATTGAAGACTCCGTCACTGCCTTGGAATTCAAAGCCTACAACGACGTTAAGACATGGGTTTCCGCTGCCATGAGCGATGCTGCTTCCTGTGACATGGGATTCAAAGACAGACCTGGCTATAAGAATCCAATTGCACCCATGACCACCACTTTTGATCAGATTTGCTCCATTATTTTGGCCATTACTCAACAGCTCTTAACTGGGGGGAATAGCAATGTCAGCAGCTAA

Protein sequence

MKSISVAMAVVLLAFSLCHAENTQQNPTLAGNDLVAKTCSSTPYNDLCKAILQSSPKSNGADLYGLAHILMDIAAENVTNIYDSINQLQNDSNIDSFMDECLTDCLESYQDAIDQIEDSVTALEFKAYNDVKTWVSAAMSDAASCDMGFKDRPGYKNPIAPMTTTFDQICSIILAITQQLLTGGNSNVSS

Homology

[Close]

BLAST of Spg028063 vs. NCBI nr
Match: XP_038880337.1 (putative invertase inhibitor [Benincasa hispida])

HSP 1 Score: 266.2 bits (679), Expect = 2.2e-67
Identity = 133/184 (72.28%), Postives = 157/184 (85.33%), Query Frame = 0


Query: 1   MKSISVAMAVVLLAFSLCHAENTQQNP----TLAGNDLVAKTCSSTPYNDLCKAILQSSP 60
           MK IS+ M V++L  SLC  +   Q P    T  GNDL+ KTCSSTPYND+CK+IL SSP
Sbjct: 1   MKPISIVMVVLVL--SLCQFQILSQTPQTPSTSGGNDLITKTCSSTPYNDMCKSILLSSP 60

Query: 61  KSNGADLYGLAHILMDIAAENVTNIYDSINQLQNDSNIDSFMDECLTDCLESYQDAIDQI 120
            SNGADLYGLA I++ IAAENV+NIY+SINQLQN +++DSF+D+CLTDCLESYQDAIDQI
Sbjct: 61  NSNGADLYGLAQIVIQIAAENVSNIYESINQLQNGTSVDSFLDDCLTDCLESYQDAIDQI 120

Query: 121 EDSVTALEFKAYNDVKTWVSAAMSDAASCDMGFKDRPGYKNPIAPMTTTFDQICSIILAI 180
           EDSVTALEFKAYNDVKTWVSAAMSDAA+CDMGFK++PGY++PIA MT  FDQIC+IIL++
Sbjct: 121 EDSVTALEFKAYNDVKTWVSAAMSDAATCDMGFKEKPGYQSPIAQMTAVFDQICNIILSM 180

[Close]

BLAST of Spg028063 vs. NCBI nr
Match: KAA0050919.1 (putative invertase inhibitor [Cucumis melo var. makuwa] >TYK10267.1 putative invertase inhibitor [Cucumis melo var. makuwa])

HSP 1 Score: 260.0 bits (663), Expect = 1.6e-65
Identity = 132/187 (70.59%), Postives = 153/187 (81.82%), Query Frame = 0


Query: 5   SVAMAVVLLAFSLCHAENTQQNP----TLAGNDLVAKTCSSTPYNDLCKAILQSSPKSNG 64
           S+A+AV  L  SLC  +   Q P    T  GNDL+ KTCSSTP+ ++CK ILQSSP S G
Sbjct: 3   SIAIAVAFLVISLCQFQILAQTPQTPTTSGGNDLITKTCSSTPHVEMCKTILQSSPNSKG 62

Query: 65  ADLYGLAHILMDIAAENVTNIYDSINQLQNDSNIDSFMDECLTDCLESYQDAIDQIEDSV 124
           ADLYGLA I+M+IAA NV+NIY+SINQLQN + +DSF+D CLTDCLESYQDAIDQIEDSV
Sbjct: 63  ADLYGLAQIVMNIAASNVSNIYESINQLQNGTTVDSFLDSCLTDCLESYQDAIDQIEDSV 122

Query: 125 TALEFKAYNDVKTWVSAAMSDAASCDMGFKDRPGYKNPIAPMTTTFDQICSIILAITQQL 184
           TALEFKAYNDV TWVSAAMSDAA+CDMGFK++ GY++PIA MTT FDQIC IILAI  +L
Sbjct: 123 TALEFKAYNDVNTWVSAAMSDAATCDMGFKEKQGYQSPIAQMTTVFDQICKIILAI-NKL 182

Query: 185 LTGGNSN 188
           L+ GNSN
Sbjct: 183 LSQGNSN 188

[Close]

BLAST of Spg028063 vs. NCBI nr
Match: XP_011659828.1 (putative invertase inhibitor [Cucumis sativus] >KGN66046.1 hypothetical protein Csa_007292 [Cucumis sativus])

HSP 1 Score: 253.4 bits (646), Expect = 1.5e-63
Identity = 125/187 (66.84%), Postives = 156/187 (83.42%), Query Frame = 0


Query: 5   SVAMAVVLLAFSLCHAENTQQNP----TLAGNDLVAKTCSSTPYNDLCKAILQSSPKSNG 64
           S+A+AV  L  SLC  +   Q P    T  GNDL++KTCSST Y+++CK ILQSSP S G
Sbjct: 3   SIAIAVAFLVVSLCQFQILAQTPQTPTTSGGNDLISKTCSSTSYSEMCKTILQSSPNSKG 62

Query: 65  ADLYGLAHILMDIAAENVTNIYDSINQLQNDSNIDSFMDECLTDCLESYQDAIDQIEDSV 124
           ADLYGLA I+M++AA+NV++IY++INQLQN +++DSF+D CLTDCLES+QDAIDQIEDSV
Sbjct: 63  ADLYGLAQIVMNVAADNVSSIYENINQLQNGTSVDSFLDSCLTDCLESFQDAIDQIEDSV 122

Query: 125 TALEFKAYNDVKTWVSAAMSDAASCDMGFKDRPGYKNPIAPMTTTFDQICSIILAITQQL 184
           TALEFKAYNDVKTW+SAAMSD A+CD GFK++ GY++PIA MT+ FDQICSIIL+I  QL
Sbjct: 123 TALEFKAYNDVKTWISAAMSDVATCDSGFKEKQGYQSPIAQMTSVFDQICSIILSI-NQL 182

Query: 185 LTGGNSN 188
           L+ GN+N
Sbjct: 183 LSQGNTN 188

[Close]

BLAST of Spg028063 vs. NCBI nr
Match: XP_022135660.1 (putative invertase inhibitor [Momordica charantia])

HSP 1 Score: 245.7 bits (626), Expect = 3.1e-61
Identity = 123/183 (67.21%), Postives = 148/183 (80.87%), Query Frame = 0


Query: 6   VAMAVVLLAFSLCH----AENTQQNPTLA----GNDLVAKTCSSTPYNDLCKAILQSSPK 65
           +++ V +L  SLC     A+  QQ    A    GN+L+ KTC+STPY D C +ILQSSPK
Sbjct: 4   ISILVPILVLSLCQFQILADQLQQQQPAASGGGGNELITKTCTSTPYKDFCISILQSSPK 63

Query: 66  SNGADLYGLAHILMDIAAENVTNIYDSINQLQNDSNIDSFMDECLTDCLESYQDAIDQIE 125
           SNGADLYGLA ILM+ AA+NV++IY+SIN LQNDS+ D+FM++CL DCLES+QDAIDQIE
Sbjct: 64  SNGADLYGLAEILMETAAQNVSSIYESINHLQNDSSPDAFMEQCLADCLESFQDAIDQIE 123

Query: 126 DSVTALEFKAYNDVKTWVSAAMSDAASCDMGFKDRPGYKNPIAPMTTTFDQICSIILAIT 181
           DSVTALEFKAYND+KTWVSAAMSDAA+CD GFKDR GY++P+A MT  FDQICSIILA+T
Sbjct: 124 DSVTALEFKAYNDIKTWVSAAMSDAATCDQGFKDRQGYQSPVAQMTAVFDQICSIILAVT 183

[Close]

BLAST of Spg028063 vs. NCBI nr
Match: XP_022970023.1 (pectinesterase inhibitor 7-like [Cucurbita maxima])

HSP 1 Score: 232.3 bits (591), Expect = 3.6e-57
Identity = 115/183 (62.84%), Postives = 143/183 (78.14%), Query Frame = 0


Query: 1   MKSISVAMAVVLLAFSLCHAENTQQNPTLAGNDLVAKTCSSTPYNDLCKAILQSSPKSNG 60
           MK ISVA+A VL+  SLC  +          N+L+ K CS T YNDLCKAILQ++P SN 
Sbjct: 1   MKPISVAVA-VLVVVSLCSFQ------AQGDNELITKACSRTAYNDLCKAILQAAPNSND 60

Query: 61  ADLYGLAHILMDIAAENVTNIYDSINQLQNDSNIDSFMDECLTDCLESYQDAIDQIEDSV 120
           ADLYGL    + IAA+NV+NIY +I++LQNDS++DSFMDECLTDCLE+YQDA DQIEDSV
Sbjct: 61  ADLYGLTQFALSIAAQNVSNIYQTIDKLQNDSSVDSFMDECLTDCLENYQDATDQIEDSV 120

Query: 121 TALEFKAYNDVKTWVSAAMSDAASCDMGFKDRPGYKNPIAPMTTTFDQICSIILAITQQL 180
           TALEFKA+NDV+TWV+AAMSD A+CD+GFKD+PGY++P+  M+  FDQICSI+L     L
Sbjct: 121 TALEFKAFNDVRTWVAAAMSDVATCDLGFKDKPGYQSPVHQMSAVFDQICSIVLTFNHCL 176

Query: 181 LTG 184
           + G
Sbjct: 181 IEG 176

[Close]

BLAST of Spg028063 vs. ExPASy Swiss-Prot
Match: A9YUH4 (Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 8.7e-14
Identity = 52/174 (29.89%), Postives = 85/174 (48.85%), Query Frame = 0


Query: 13  LAFSLCHAENTQQNPTLAGNDLVAKTCSSTPYN------DLCKAILQSSPKSNGADLYGL 72
           L+FSLC          L   D+V  TC            D C   L + PKS+ ADL GL
Sbjct: 3   LSFSLCIF-------FLISADIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHSADLQGL 62

Query: 73  AHILMDIAAENVTNIYDSINQLQNDSNIDSFMDECLTDCLESYQDAIDQIEDSVTALEFK 132
             I  ++A +  + I   I ++   S +D  + + L DC+  Y DA   +++++   + K
Sbjct: 63  GVISANLAIQQGSKIQTFIGRILK-SKVDPALKKYLNDCVGLYADAKSSVQEAIADFKSK 122

Query: 133 AYNDVKTWVSAAMSDAASCDMGFKDRPGYKNPIAPMTTTFDQICSIILAITQQL 181
            Y      +SAA+ D+ +C+ GFK++ G  +P+      + Q+ +I LAIT+ L
Sbjct: 123 DYASANVKMSAALDDSVTCEDGFKEKKGIASPVTKENKDYVQLTAISLAITKLL 168

[Close]

BLAST of Spg028063 vs. ExPASy Swiss-Prot
Match: Q8GT41 (Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 3.3e-13
Identity = 46/154 (29.87%), Postives = 79/154 (51.30%), Query Frame = 0


Query: 33  DLVAKTCSSTPYN------DLCKAILQSSPKSNGADLYGLAHILMDIAAENVTNIYDSIN 92
           D+V  TC            D C   L + PKS+ ADL GL  I  ++A ++ + I   I 
Sbjct: 25  DIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHTADLQGLGVISANLAIQHGSKIQTFIG 84

Query: 93  QLQNDSNIDSFMDECLTDCLESYQDAIDQIEDSVTALEFKAYNDVKTWVSAAMSDAASCD 152
           ++   S +D  + + L DC+  Y DA   +++++   + K Y      +SAA+ D+ +C+
Sbjct: 85  RILK-SKVDPALKKYLNDCVGLYADAKSSVQEAIADFKSKDYASANVKMSAALDDSVTCE 144

Query: 153 MGFKDRPGYKNPIAPMTTTFDQICSIILAITQQL 181
            GFK++ G  +P+      + Q+ +I LAIT+ L
Sbjct: 145 DGFKEKKGIVSPVTKENKDYVQLTAISLAITKLL 177

[Close]

BLAST of Spg028063 vs. ExPASy Swiss-Prot
Match: O49603 (Cell wall / vacuolar inhibitor of fructosidase 2 OS=Arabidopsis thaliana OX=3702 GN=C/VIF2 PE=1 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 8.4e-09
Identity = 49/183 (26.78%), Postives = 83/183 (45.36%), Query Frame = 0


Query: 1   MKSISVAMAVVLLAFSLCHAENTQQNPTLAGNDLVAKTCSSTPYNDLCKAILQSSPKSNG 60
           M S  + + +V L FS     + + N T     ++  TC +T Y   C + L+S P+S  
Sbjct: 1   MASSLIFLLLVTLTFSASTLISAKSNTT----TIIESTCKTTNYYKFCVSALKSDPRSPT 60

Query: 61  ADLYGLAHILMDIAAENVTNIYDSINQLQNDSNIDSFMDECLTDCLESYQDAIDQIEDSV 120
           AD  GLA I++ +   N T+  + I    + +  D+ + + L DC E Y  A D +  ++
Sbjct: 61  ADTKGLASIMVGVGMTNATSTANYIAGNLSATVKDTVLKKVLQDCSEKYALAADSLRLTI 120

Query: 121 TALEFKAYNDVKTWVSAAMSDAASCDMGFKDRPGYKNP--IAPMTTTFDQICSIILAITQ 180
             L+ +AY+     V AA      C   F+   G   P  I     +  +IC ++  I  
Sbjct: 121 QDLDDEAYDYASMHVLAAQDYPNVCRNIFRRVKGLAYPVEIRRREASLRRICGVVSGILD 179

Query: 181 QLL 182
           +L+
Sbjct: 181 RLV 179

[Close]

BLAST of Spg028063 vs. ExPASy Swiss-Prot
Match: O49298 (Probable pectinesterase/pectinesterase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=PME6 PE=2 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 2.5e-05
Identity = 29/118 (24.58%), Postives = 60/118 (50.85%), Query Frame = 0


Query: 38  TCSSTPYNDLCKAILQSSP---KSNGADLYGLAHILMDIAAENVTNIYDSINQLQNDSNI 97
           +C  TPY  +C   + +SP     +  D +    +++    +    ++  ++ L+   ++
Sbjct: 37  SCKQTPYPSVCDHHMSNSPLKTLDDQTDGFTFHDLVVSSTMDQAVQLHRLVSSLKQHHSL 96

Query: 98  DSFMDECLTDCLESYQDAIDQIEDSVTAL-EFKAYNDVKTWVSAAMSDAASCDMGFKD 152
                  L DCLE Y+D IDQ+  S  +  ++ + +D +T +SAA+++  +C  GF+D
Sbjct: 97  HKHATSALFDCLELYEDTIDQLNHSRRSYGQYSSPHDRQTSLSAAIANQDTCRNGFRD 154

[Close]

BLAST of Spg028063 vs. ExPASy Swiss-Prot
Match: Q9SB38 (Pectinesterase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=PMEI4 PE=2 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 2.5e-05
Identity = 39/141 (27.66%), Postives = 67/141 (47.52%), Query Frame = 0


Query: 23  TQQNPTLAGNDLVAKTCSSTPYNDLCKAILQSSPKSNGADLYGLAHILMDI---AAENVT 82
           T QN T      V   C+ST Y  +C   L S   +  +D   L    +++   +A+N T
Sbjct: 30  TYQNHT----TYVKTACNSTTYPTMCYNCLSSYSSTIKSDPIKLCTTSLNLNVKSAKNAT 89

Query: 83  NIYDSINQLQNDSNIDSFMDECLTDCLESYQDAIDQIEDSVTALEF---------KAYND 142
            +    N LQ      S     L DC++  +D ID+++ +V  +++         +   +
Sbjct: 90  LVVS--NLLQKAKAAKSHEVSILKDCVDEMKDTIDELKQAVAEMKYVRGGGKTTEEHLKN 149

Query: 143 VKTWVSAAMSDAASCDMGFKD 152
           VKTWVS+A++D  +C  GF++
Sbjct: 150 VKTWVSSALTDEGTCTDGFEE 164

[Close]

BLAST of Spg028063 vs. ExPASy TrEMBL
Match: A0A5A7UBK3 (Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004170 PE=4 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 7.7e-66
Identity = 132/187 (70.59%), Postives = 153/187 (81.82%), Query Frame = 0


Query: 5   SVAMAVVLLAFSLCHAENTQQNP----TLAGNDLVAKTCSSTPYNDLCKAILQSSPKSNG 64
           S+A+AV  L  SLC  +   Q P    T  GNDL+ KTCSSTP+ ++CK ILQSSP S G
Sbjct: 3   SIAIAVAFLVISLCQFQILAQTPQTPTTSGGNDLITKTCSSTPHVEMCKTILQSSPNSKG 62

Query: 65  ADLYGLAHILMDIAAENVTNIYDSINQLQNDSNIDSFMDECLTDCLESYQDAIDQIEDSV 124
           ADLYGLA I+M+IAA NV+NIY+SINQLQN + +DSF+D CLTDCLESYQDAIDQIEDSV
Sbjct: 63  ADLYGLAQIVMNIAASNVSNIYESINQLQNGTTVDSFLDSCLTDCLESYQDAIDQIEDSV 122

Query: 125 TALEFKAYNDVKTWVSAAMSDAASCDMGFKDRPGYKNPIAPMTTTFDQICSIILAITQQL 184
           TALEFKAYNDV TWVSAAMSDAA+CDMGFK++ GY++PIA MTT FDQIC IILAI  +L
Sbjct: 123 TALEFKAYNDVNTWVSAAMSDAATCDMGFKEKQGYQSPIAQMTTVFDQICKIILAI-NKL 182

Query: 185 LTGGNSN 188
           L+ GNSN
Sbjct: 183 LSQGNSN 188

[Close]

BLAST of Spg028063 vs. ExPASy TrEMBL
Match: A0A0A0M1H8 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G569235 PE=4 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 7.2e-64
Identity = 125/187 (66.84%), Postives = 156/187 (83.42%), Query Frame = 0


Query: 5   SVAMAVVLLAFSLCHAENTQQNP----TLAGNDLVAKTCSSTPYNDLCKAILQSSPKSNG 64
           S+A+AV  L  SLC  +   Q P    T  GNDL++KTCSST Y+++CK ILQSSP S G
Sbjct: 3   SIAIAVAFLVVSLCQFQILAQTPQTPTTSGGNDLISKTCSSTSYSEMCKTILQSSPNSKG 62

Query: 65  ADLYGLAHILMDIAAENVTNIYDSINQLQNDSNIDSFMDECLTDCLESYQDAIDQIEDSV 124
           ADLYGLA I+M++AA+NV++IY++INQLQN +++DSF+D CLTDCLES+QDAIDQIEDSV
Sbjct: 63  ADLYGLAQIVMNVAADNVSSIYENINQLQNGTSVDSFLDSCLTDCLESFQDAIDQIEDSV 122

Query: 125 TALEFKAYNDVKTWVSAAMSDAASCDMGFKDRPGYKNPIAPMTTTFDQICSIILAITQQL 184
           TALEFKAYNDVKTW+SAAMSD A+CD GFK++ GY++PIA MT+ FDQICSIIL+I  QL
Sbjct: 123 TALEFKAYNDVKTWISAAMSDVATCDSGFKEKQGYQSPIAQMTSVFDQICSIILSI-NQL 182

Query: 185 LTGGNSN 188
           L+ GN+N
Sbjct: 183 LSQGNTN 188

[Close]

BLAST of Spg028063 vs. ExPASy TrEMBL
Match: A0A6J1C1M8 (putative invertase inhibitor OS=Momordica charantia OX=3673 GN=LOC111007563 PE=4 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 1.5e-61
Identity = 123/183 (67.21%), Postives = 148/183 (80.87%), Query Frame = 0


Query: 6   VAMAVVLLAFSLCH----AENTQQNPTLA----GNDLVAKTCSSTPYNDLCKAILQSSPK 65
           +++ V +L  SLC     A+  QQ    A    GN+L+ KTC+STPY D C +ILQSSPK
Sbjct: 4   ISILVPILVLSLCQFQILADQLQQQQPAASGGGGNELITKTCTSTPYKDFCISILQSSPK 63

Query: 66  SNGADLYGLAHILMDIAAENVTNIYDSINQLQNDSNIDSFMDECLTDCLESYQDAIDQIE 125
           SNGADLYGLA ILM+ AA+NV++IY+SIN LQNDS+ D+FM++CL DCLES+QDAIDQIE
Sbjct: 64  SNGADLYGLAEILMETAAQNVSSIYESINHLQNDSSPDAFMEQCLADCLESFQDAIDQIE 123

Query: 126 DSVTALEFKAYNDVKTWVSAAMSDAASCDMGFKDRPGYKNPIAPMTTTFDQICSIILAIT 181
           DSVTALEFKAYND+KTWVSAAMSDAA+CD GFKDR GY++P+A MT  FDQICSIILA+T
Sbjct: 124 DSVTALEFKAYNDIKTWVSAAMSDAATCDQGFKDRQGYQSPVAQMTAVFDQICSIILAVT 183

[Close]

BLAST of Spg028063 vs. ExPASy TrEMBL
Match: A0A6J1I2M6 (pectinesterase inhibitor 7-like OS=Cucurbita maxima OX=3661 GN=LOC111469035 PE=4 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 1.7e-57
Identity = 115/183 (62.84%), Postives = 143/183 (78.14%), Query Frame = 0


Query: 1   MKSISVAMAVVLLAFSLCHAENTQQNPTLAGNDLVAKTCSSTPYNDLCKAILQSSPKSNG 60
           MK ISVA+A VL+  SLC  +          N+L+ K CS T YNDLCKAILQ++P SN 
Sbjct: 1   MKPISVAVA-VLVVVSLCSFQ------AQGDNELITKACSRTAYNDLCKAILQAAPNSND 60

Query: 61  ADLYGLAHILMDIAAENVTNIYDSINQLQNDSNIDSFMDECLTDCLESYQDAIDQIEDSV 120
           ADLYGL    + IAA+NV+NIY +I++LQNDS++DSFMDECLTDCLE+YQDA DQIEDSV
Sbjct: 61  ADLYGLTQFALSIAAQNVSNIYQTIDKLQNDSSVDSFMDECLTDCLENYQDATDQIEDSV 120

Query: 121 TALEFKAYNDVKTWVSAAMSDAASCDMGFKDRPGYKNPIAPMTTTFDQICSIILAITQQL 180
           TALEFKA+NDV+TWV+AAMSD A+CD+GFKD+PGY++P+  M+  FDQICSI+L     L
Sbjct: 121 TALEFKAFNDVRTWVAAAMSDVATCDLGFKDKPGYQSPVHQMSAVFDQICSIVLTFNHCL 176

Query: 181 LTG 184
           + G
Sbjct: 181 IEG 176

[Close]

BLAST of Spg028063 vs. ExPASy TrEMBL
Match: A0A6J1HUH7 (pectinesterase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111467924 PE=4 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 7.3e-56
Identity = 114/193 (59.07%), Postives = 151/193 (78.24%), Query Frame = 0


Query: 1   MKSISVAMAVVLLAFSLCHAE---NTQQNPTLAGNDLVAKTCSSTPYNDLCKAILQSSPK 60
           MK IS+A A+ +   S+ H +   +  Q P L   DL+ KTCSSTPYNDLC +IL+SSP 
Sbjct: 1   MKLISIA-AMAITVLSMTHFQILADNPQKPFLTNADLITKTCSSTPYNDLCLSILESSPN 60

Query: 61  SNGADLYGLAHILMDIAAENVTNIYDSINQLQNDSNIDSFMDECLTDCLESYQDAIDQIE 120
           SNG DLYGL  I++ IAAENV+ I++ + +LQN++ +D+FMDECLTDCLE+YQDA DQIE
Sbjct: 61  SNGTDLYGLTEIMLYIAAENVSLIHEHLMELQNNTEMDTFMDECLTDCLENYQDATDQIE 120

Query: 121 DSVTALEFKAYNDVKTWVSAAMSDAASCDMGFKDRPGYKNPIAPMTTTFDQICSIILAIT 180
           DS+TALEFKAY+D+KTWV+AAM+D A C+ GFK++P +K+PI  M+  FDQICSIILA+T
Sbjct: 121 DSITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMT 180

Query: 181 QQLLTGGNSNVSS 191
             +L+ GN N++S
Sbjct: 181 -NILSQGNRNMNS 191

[Close]

BLAST of Spg028063 vs. TAIR 10
Match: AT1G55770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 70.5 bits (171), Expect = 1.7e-12
Identity = 41/155 (26.45%), Postives = 80/155 (51.61%), Query Frame = 0


Query: 30  AGNDLVAKTCSSTPYNDLCKAILQSSPKSNGADLYGLAH----ILMDIAAENVTNIYDSI 89
           A + +++  C  +   +LC + + S P+S   +  G ++    I +  A+ N +     I
Sbjct: 22  ANSGMISDLCKHSDDPNLCLSSITSRPES--GEFAGTSNQIEIIAISAASANASATSSYI 81

Query: 90  NQLQNDSNIDSFMDECLTDCLESYQDAIDQIEDSVTALEFKAYNDVKTWVSAAMSDAASC 149
            Q  ++ +++  +++ L DC + YQDA++Q++DS++A+   A+ DV  W+SAA+S   SC
Sbjct: 82  KQKLSNEDLEPAIEDTLEDCQKDYQDAVEQLDDSISAMLADAHTDVDVWLSAAISAIESC 141

Query: 150 DMGFKDRPGYKNPIAPMTTTFDQICSIILAITQQL 181
                 R G    ++     F ++C   L I + L
Sbjct: 142 GSALGSRAGNDAELSQRNEVFLKLCKNALMINKML 174

[Close]

BLAST of Spg028063 vs. TAIR 10
Match: AT4G02250.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 69.7 bits (169), Expect = 2.9e-12
Identity = 39/129 (30.23%), Postives = 66/129 (51.16%), Query Frame = 0


Query: 48  CKAILQSSPKSNGADLYGLAHILMDIAAENVTNIYDSINQLQNDSNIDSFMDECLTDCLE 107
           C A L S P++  A    L  I + IA+ N ++    I       N++  +++ L DC +
Sbjct: 26  CVASLTSRPEAATATAPKLGVIALSIASSNASDTSFYIKAKLKQKNLEPALEDTLDDCSK 85

Query: 108 SYQDAIDQIEDSVTALEFKAYNDVKTWVSAAMSDAASCDMGFKDRPGYKNPIAPMTTTFD 167
           +Y DA+ Q++DS+ AL   ++ DV  W++ A+SD  +C+    DR G    +A   T   
Sbjct: 86  NYLDAVAQLDDSLAALMQNSFIDVDIWLNTAISDGEACENALNDRAGNDAELARRNTNLL 145

Query: 168 QICSIILAI 177
           ++C   L I
Sbjct: 146 KLCKDALLI 154

[Close]

BLAST of Spg028063 vs. TAIR 10
Match: AT5G38610.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 64.3 bits (155), Expect = 1.2e-10
Identity = 50/159 (31.45%), Postives = 79/159 (49.69%), Query Frame = 0


Query: 4   ISVAMAVVLLAFSLCHAENTQQNPTLAGNDLVAKTCSST-------PYNDLCKAILQSSP 63
           +S    ++ L F +   ++T        +D++ +TC S         YN  C + L +SP
Sbjct: 3   LSQVFYIIFLFFLVSQVKST--------SDMIDQTCKSCAAKSTIFDYN-FCVSSLNNSP 62

Query: 64  KS--NGADLYGLAHILMDIAAENVTNIYDSINQLQNDSNIDSFMDECLTDCLESYQDAID 123
            +  +  +L  LA + M  A +N T    +I QL    +  SF   CL DCLE Y+DA D
Sbjct: 63  IALPSPTNLSSLALVPMLQALDNATATASTIQQLLISDDDGSFRSACLRDCLELYEDATD 122

Query: 124 QIEDSVTA-LEFKAYNDVKTWVSAAMSDAASCDMGFKDR 153
           ++E++V   +  K    V   VSAAM  A +C+ GF++R
Sbjct: 123 RLEEAVRVFITRKELGTVNVMVSAAMESAVTCENGFRER 152

[Close]

BLAST of Spg028063 vs. TAIR 10
Match: AT3G47670.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 63.5 bits (153), Expect = 2.0e-10
Identity = 54/193 (27.98%), Postives = 89/193 (46.11%), Query Frame = 0


Query: 6   VAMAVVLLAFSLCHAENTQQNPTLAGNDLVAKTCSSTPYNDLCKAILQSSPKSNGADL-- 65
           +   V L A +  H  +   +P ++   L    CS T Y + C   L SS   + + L  
Sbjct: 87  ILSVVALTAGAFTH--SPPHHPPISSASL-KSLCSVTRYPETCFNSLSSSLNESDSKLNP 146

Query: 66  YGLAHILMDIAAENVTNI---YDSINQLQNDSNIDSFMDECLTDCLESYQDAIDQIEDSV 125
             +  + + +AA+ ++N+   + SIN +  D+ +         DC++ Y DA+ Q+ DS+
Sbjct: 147 ESILELSLRVAAKEISNLSISFRSINDMPEDAAVG--------DCVKLYTDALSQLNDSI 206

Query: 126 TALEFK------------AYNDVKTWVSAAMSDAASCDMGFKDRPG-YKNPIAPMTTTFD 181
           T +E K               DVKTW+SAAM+D  +C  G ++      N I       +
Sbjct: 207 TEIERKKKKGGNNWLTEEVVGDVKTWISAAMTDGETCSDGIEEMGTIVGNEIKKKMEMAN 266

[Close]

BLAST of Spg028063 vs. TAIR 10
Match: AT5G64620.1 (cell wall / vacuolar inhibitor of fructosidase 2 )

HSP 1 Score: 62.0 bits (149), Expect = 6.0e-10
Identity = 49/183 (26.78%), Postives = 83/183 (45.36%), Query Frame = 0


Query: 1   MKSISVAMAVVLLAFSLCHAENTQQNPTLAGNDLVAKTCSSTPYNDLCKAILQSSPKSNG 60
           M S  + + +V L FS     + + N T     ++  TC +T Y   C + L+S P+S  
Sbjct: 1   MASSLIFLLLVTLTFSASTLISAKSNTT----TIIESTCKTTNYYKFCVSALKSDPRSPT 60

Query: 61  ADLYGLAHILMDIAAENVTNIYDSINQLQNDSNIDSFMDECLTDCLESYQDAIDQIEDSV 120
           AD  GLA I++ +   N T+  + I    + +  D+ + + L DC E Y  A D +  ++
Sbjct: 61  ADTKGLASIMVGVGMTNATSTANYIAGNLSATVKDTVLKKVLQDCSEKYALAADSLRLTI 120

Query: 121 TALEFKAYNDVKTWVSAAMSDAASCDMGFKDRPGYKNP--IAPMTTTFDQICSIILAITQ 180
             L+ +AY+     V AA      C   F+   G   P  I     +  +IC ++  I  
Sbjct: 121 QDLDDEAYDYASMHVLAAQDYPNVCRNIFRRVKGLAYPVEIRRREASLRRICGVVSGILD 179

Query: 181 QLL 182
           +L+
Sbjct: 181 RLV 179

The following BLAST results are available for this feature:

BLAST of Spg028063 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038880337.1	2.2e-67	72.28	putative invertase inhibitor [Benincasa hispida]	[more]
KAA0050919.1	1.6e-65	70.59	putative invertase inhibitor [Cucumis melo var. makuwa] >TYK10267.1 putative inv...	[more]
XP_011659828.1	1.5e-63	66.84	putative invertase inhibitor [Cucumis sativus] >KGN66046.1 hypothetical protein ...	[more]
XP_022135660.1	3.1e-61	67.21	putative invertase inhibitor [Momordica charantia]	[more]
XP_022970023.1	3.6e-57	62.84	pectinesterase inhibitor 7-like [Cucurbita maxima]	[more]

BLAST of Spg028063 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A9YUH4	8.7e-14	29.89	Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1	[more]
Q8GT41	3.3e-13	29.87	Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1	[more]
O49603	8.4e-09	26.78	Cell wall / vacuolar inhibitor of fructosidase 2 OS=Arabidopsis thaliana OX=3702...	[more]
O49298	2.5e-05	24.58	Probable pectinesterase/pectinesterase inhibitor 6 OS=Arabidopsis thaliana OX=37...	[more]
Q9SB38	2.5e-05	27.66	Pectinesterase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=PMEI4 PE=2 SV=1	[more]

BLAST of Spg028063 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UBK3	7.7e-66	70.59	Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A0A0M1H8	7.2e-64	66.84	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G569235 PE=4 S...	[more]
A0A6J1C1M8	1.5e-61	67.21	putative invertase inhibitor OS=Momordica charantia OX=3673 GN=LOC111007563 PE=4...	[more]
A0A6J1I2M6	1.7e-57	62.84	pectinesterase inhibitor 7-like OS=Cucurbita maxima OX=3661 GN=LOC111469035 PE=4...	[more]
A0A6J1HUH7	7.3e-56	59.07	pectinesterase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111467924 PE=4 S...	[more]

BLAST of Spg028063 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G55770.1	1.7e-12	26.45	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G02250.1	2.9e-12	30.23	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G38610.1	1.2e-10	31.45	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT3G47670.1	2.0e-10	27.98	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G64620.1	6.0e-10	26.78	cell wall / vacuolar inhibitor of fructosidase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 106..126
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 13..180
None	No IPR available	PANTHER	PTHR31080:SF1	OS05G0360900 PROTEIN	coord: 13..180
None	No IPR available	CDD	cd15801	PMEI-like_1	coord: 34..180 e-value: 7.23598E-53 score: 164.042
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 30..176 e-value: 1.6E-33 score: 127.3
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 33..176 e-value: 9.4E-35 score: 120.2
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 5..180 e-value: 7.0E-42 score: 140.7
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 30..181 e-value: 2.2E-43 score: 149.8
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 32..180

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg028063.1	Spg028063.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity