Spg027915 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg027915
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionReverse transcriptase domain-containing protein
Locationscaffold2: 38880849 .. 38881881 (-)
RNA-Seq ExpressionSpg027915
SyntenySpg027915
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGACAACAACGATTGGAATGAGGACCTTATTAGACAAAACTTCATCCCCCAAGACGCAAATGACATTTTAAACATGCCTACGGGAGGGAAGGACTCAAAAGACGAGATTTTCTGGCACCCGGTCAAGAATGGAATATTCTTGGTAAAGAGTGCGTACCATCTAGCGACCGACACAATTTCCAATTTGGAAGCTTCTCAATCGGATGAAAGAAAATCCTCTTCCCTTTGGAAGAGCATTTGGGCTATGGACTCTCTTCCTAGGACTAAAGTTTGTGTGTGGAAGATCCTAAACGATATCATCTCTACCAAGACCAACATTCAATCCAAGGGGATCGATATCAATCCAATGTGCTCTTTATGCGGGAAAAAAAAAGGAAACTACCTCCCACCTAATATGGGAGTGTAGAATTCCTAAAGGTATGTGGTCCCTTTTTCTTCCAAATCTCCTGACTTCTCCTTTCTTTGGGTGCAGGATGGAGCGGCCTCCAATGGACATATGGAGTTGGATGACTGATAATCTATCAAAGAAGGAGCTGGAAACAGGATGCATAATCATGTGGAGTATCTGGACTTTTAGAAATAGAATTACAAACGCAAACCAAATCACGATCCCAACATCGAATTATTTGCAGAAAGCCATTGAAGCTAATCTAATCGATTGACGAAATACTCACAGTAAACCACCGGCTCCGTCGAAGGCGAGGAACCACACGAGTCACGCTTGCTGGGAGAGACCGAAACTGGGCCATTGGAAATTGAATTCGGATGCCGCTTGGAACAAGGATAAAGGTTGCGGAGGGCTGGGTTGGGTGGTCCGTGACTCGTCTGGGTCCACGATCTGCTTCGGAATGAAGACAATTTACAAAGAATGGCTGGTTAATCTTCTGGAAGCGAAAGCAATTGCTGAAGGCCTCAATGAAATTATTCATACCTGCGTCAGAGAACGTATTCCGCTGGAAGTTGAAACGGATGCGTTGGGGGTGATTAAAGTGATCAACGGAGACGCGAGCTCGATCTCTCCAACCTGA

mRNA sequence

ATGGATGACAACAACGATTGGAATGAGGACCTTATTAGACAAAACTTCATCCCCCAAGACGCAAATGACATTTTAAACATGCCTACGGGAGGGAAGGACTCAAAAGACGAGATTTTCTGGCACCCGGTCAAGAATGGAATATTCTTGGTAAAGAGTGCGTACCATCTAGCGACCGACACAATTTCCAATTTGGAAGCTTCTCAATCGGATGAAAGAAAATCCTCTTCCCTTTGGAAGAGCATTTGGGCTATGGACTCTCTTCCTAGGACTAAAGTTTGTGTGTGGAAGATCCTAAACGATATCATCTCTACCAAGACCAACATTCAATCCAAGGGGATCGATATCAATCCAATGATGGAGCGGCCTCCAATGGACATATGGAGTTGGATGACTGATAATCTATCAAAGAAGGAGCTGGAAACAGGATGCATAATCATTAAACCACCGGCTCCGTCGAAGGCGAGGAACCACACGAGTCACGCTTGCTGGGAGAGACCGAAACTGGGCCATTGGAAATTGAATTCGGATGCCGCTTGGAACAAGGATAAAGGTTGCGGAGGGCTGGGTTGGGTGGTCCGTGACTCGTCTGGGTCCACGATCTGCTTCGGAATGAAGACAATTTACAAAGAATGGCTGGTTAATCTTCTGGAAGCGAAAGCAATTGCTGAAGGCCTCAATGAAATTATTCATACCTGCGTCAGAGAACGTATTCCGCTGGAAGTTGAAACGGATGCGTTGGGGGTGATTAAAGTGATCAACGGAGACGCGAGCTCGATCTCTCCAACCTGA

Coding sequence (CDS)

ATGGATGACAACAACGATTGGAATGAGGACCTTATTAGACAAAACTTCATCCCCCAAGACGCAAATGACATTTTAAACATGCCTACGGGAGGGAAGGACTCAAAAGACGAGATTTTCTGGCACCCGGTCAAGAATGGAATATTCTTGGTAAAGAGTGCGTACCATCTAGCGACCGACACAATTTCCAATTTGGAAGCTTCTCAATCGGATGAAAGAAAATCCTCTTCCCTTTGGAAGAGCATTTGGGCTATGGACTCTCTTCCTAGGACTAAAGTTTGTGTGTGGAAGATCCTAAACGATATCATCTCTACCAAGACCAACATTCAATCCAAGGGGATCGATATCAATCCAATGATGGAGCGGCCTCCAATGGACATATGGAGTTGGATGACTGATAATCTATCAAAGAAGGAGCTGGAAACAGGATGCATAATCATTAAACCACCGGCTCCGTCGAAGGCGAGGAACCACACGAGTCACGCTTGCTGGGAGAGACCGAAACTGGGCCATTGGAAATTGAATTCGGATGCCGCTTGGAACAAGGATAAAGGTTGCGGAGGGCTGGGTTGGGTGGTCCGTGACTCGTCTGGGTCCACGATCTGCTTCGGAATGAAGACAATTTACAAAGAATGGCTGGTTAATCTTCTGGAAGCGAAAGCAATTGCTGAAGGCCTCAATGAAATTATTCATACCTGCGTCAGAGAACGTATTCCGCTGGAAGTTGAAACGGATGCGTTGGGGGTGATTAAAGTGATCAACGGAGACGCGAGCTCGATCTCTCCAACCTGA

Protein sequence

MDDNNDWNEDLIRQNFIPQDANDILNMPTGGKDSKDEIFWHPVKNGIFLVKSAYHLATDTISNLEASQSDERKSSSLWKSIWAMDSLPRTKVCVWKILNDIISTKTNIQSKGIDINPMMERPPMDIWSWMTDNLSKKELETGCIIIKPPAPSKARNHTSHACWERPKLGHWKLNSDAAWNKDKGCGGLGWVVRDSSGSTICFGMKTIYKEWLVNLLEAKAIAEGLNEIIHTCVRERIPLEVETDALGVIKVINGDASSISPT
Homology
BLAST of Spg027915 vs. NCBI nr
Match: EEC68887.1 (hypothetical protein OsI_37529 [Oryza sativa Indica Group])

HSP 1 Score: 110.9 bits (276), Expect = 1.7e-20
Identity = 82/280 (29.29%), Postives = 126/280 (45.00%), Query Frame = 0

Query: 3    DNNDWNEDLIRQNFIPQDANDILNMPTGGKDSKDEIFWHPVKNGIFLVKSAYHLATDTIS 62
            ++  W+   I Q F   DA  ILN+    +  +D I WHP KNG+F V+SAY LA   ++
Sbjct: 1423 EDGSWDVPKIHQYFHNLDAEVILNICISSRSEEDFIAWHPDKNGMFSVRSAYRLAAQLVN 1482

Query: 63   NLEASQSDERKSSSLWKSIWAMDSLPRTKVCVWKILNDIISTKTN--------------- 122
              E+S S     +  W+ IW      + K+  W++ ++ ++T                  
Sbjct: 1483 IEESSSSGTNNINKAWEMIWKCKVPQKVKIFAWRVASNCLATMAMFLMTLWRNWYVRNEL 1542

Query: 123  IQSKGIDINPMMERPPMDIWSWM-----------TDNLSKKELETGCIIIKPPAPSKARN 182
            I  K     P  E     I S++            D +  K +     +   P      N
Sbjct: 1543 IHGKSA---PPTETSQRFIQSYVDLLFQIRQAPQADLVKGKHVVRTVPLKGGPKYRVLNN 1602

Query: 183  HTSHACWERPKLGHWKLNSDAAWNKDKGCGGLGWVVRDSSGSTICFGMKTIYKEWLVNLL 242
            H    CWERPK G  KLN D +++   G GGLG ++R+S+G  I    K +  E   N L
Sbjct: 1603 H--QPCWERPKDGWMKLNVDGSFDASSGKGGLGMILRNSAGDIIFTSCKPL--ERCNNPL 1662

Query: 243  EA--KAIAEGLNEIIHTCVRERIPLEVETDALGVIKVING 255
            E+  +A  EGL   IH  +   +P++VETD   V++++ G
Sbjct: 1663 ESELRACVEGLKLAIHWTL---LPIQVETDCASVVQLLQG 1692

BLAST of Spg027915 vs. NCBI nr
Match: CAB4313148.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 110.2 bits (274), Expect = 2.8e-20
Identity = 77/300 (25.67%), Postives = 129/300 (43.00%), Query Frame = 0

Query: 7   WNEDLIRQNFIPQDANDILNMPTGGKDSKDEIFWHPVKNGIFLVKSAYHLATDTISNLE- 66
           W ED+I + F+P +A  IL++P       D++ WH   +G + V+S+Y LA     N E 
Sbjct: 13  WKEDVINRCFVPDEARCILSIPINKWGCPDKLIWHFSMHGQYTVRSSYELALHLQRNGEL 72

Query: 67  -----ASQSDERKSSSLWKSIWAMDSLPRTKVCVWKILNDIISTKTNIQSK--GIDINPM 126
                +  S     SS+WKS W  D+ P+ K  +W+  ++I++ + N++ +   +D+N +
Sbjct: 73  GHKGASEASGSYSQSSVWKSFWHADAPPKLKAFIWRGCSNILAVRHNLRRRPLQLDVNNL 132

Query: 127 MERPPMDIWSWMTDNLSKKELETGCI---------------------------------- 186
                +  W ++   ++  E     I                                  
Sbjct: 133 AAEDFISTWRYIMTMINSSEHAAEAIQWFVFGLWRIWKARNSVVFDGVQADPAEAVHLLL 192

Query: 187 -------IIK---PPAPSKARNHTSHACWERPKLGHWKLNSDAAWNKDKGCGGLGWVVRD 246
                  I K    P P +  +      W +P  G  K+N DAAW+  + CGG+GWV+RD
Sbjct: 193 QQVSEYRIAKQNLQPQPLQPASSPVPQSWCKPPPGFLKVNCDAAWSAQRLCGGVGWVIRD 252

Query: 247 SSGSTICFGMKTIYKEWLVNLLEAKAIAEGLNEIIHTCVRERIPLEVETDALGVIKVING 255
           S G  +C G +         ++E  AI   L+E  H  +   I   VE+D+   I ++NG
Sbjct: 253 SFGLLLCAGGQGDLHGSSALMMELLAIRHALSECSHFHLTNVI---VESDSQMGISMLNG 309

BLAST of Spg027915 vs. NCBI nr
Match: CAB4279811.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 106.3 bits (264), Expect = 4.1e-19
Identity = 86/297 (28.96%), Postives = 128/297 (43.10%), Query Frame = 0

Query: 7    WNEDLIRQNFIPQDANDILNMPTGGKDSKDEIFWHPVKNGIFLVKSAYHLATDTISNLEA 66
            W+ DL+ + F P +A  I  +P       D+  WH   +G + V+S Y +A     N E 
Sbjct: 859  WDMDLVNRCFNPNEAKCISTIPISRWGCPDKKVWHYTSHGGYTVRSGYAVALTLRRNGEL 918

Query: 67   SQSDERKSSS------LWKSIWAMDSLPRTKVCVWKILNDIISTKTNIQSKGIDINPM-- 126
             +  E +SS       +WKSIW +   P+ +  +WK   ++++ K N+   GID+     
Sbjct: 919  GRKAEGESSQGDKQRRIWKSIWGLQVPPKIRTFLWKCCRNVLAVKENLLHWGIDVETTCA 978

Query: 127  ---MERPPMD-IWSWMTDNLSKK------------------------------ELETGCI 186
                E    D I  W  +NL KK                              E  T  +
Sbjct: 979  LCGQEGESQDFIVCW--ENLLKKYEQVEHCNDILQGTVIEPHVAVECFLNQVHEFRTAQM 1038

Query: 187  IIKP-----PAPSKARNHTSHACWERPKLGHWKLNSDAAWNKDKGCGGLGWVVRDSSGST 246
              KP     P P + +  T    W +   G  K+N DAAW      GG+GWVVRDS G  
Sbjct: 1039 HGKPLINELPRPPEIQAETV-TSWRKLPYGVLKVNCDAAWVSQTQMGGVGWVVRDSYGWM 1098

Query: 247  ICFGMKTIYKEWLVNLLEAKAIAEGLNEIIHTCVRERI-PLEVETDALGVIKVINGD 256
            +  G K   +      +EAKAI E L      CV+  +  LEVE+D+L +I+++ G+
Sbjct: 1099 VQAGCKGDMRGGSALAMEAKAIREAL----LACVQSGLSKLEVESDSLQLIRMLGGE 1148

BLAST of Spg027915 vs. NCBI nr
Match: PNT78255.1 (hypothetical protein BRADI_1g76105v3 [Brachypodium distachyon])

HSP 1 Score: 101.7 bits (252), Expect = 1.0e-17
Identity = 72/262 (27.48%), Postives = 122/262 (46.56%), Query Frame = 0

Query: 1   MDDNNDWNEDLIRQNFIPQDANDILNMPTGGKDSKDEIFWHPVKNGIFLVKSAYHLATDT 60
           +D+N  W ++L+R+ F+P D++ IL + T  + + D + WHP K G F V+SAY L  D+
Sbjct: 107 LDENGGWRQELVRRIFLPADSDAILRIKTSPRLAPDFLAWHPEKMGSFSVRSAYRLGVDS 166

Query: 61  ---ISNLEASQSDERKSSSLWKSIWAMDSLPRTKVCVWKILNDIISTKTNIQSKGIDINP 120
               ++  A+ S    S   W  IW   + P+ K+  WK  N+ ++T+ N + +G+    
Sbjct: 167 SKLTNDRTATSSHPDGSDPCWNKIWTCIAPPKVKIFAWKAANNALATEENKKRRGM---- 226

Query: 121 MMERPPMDIWSWMTDNLSKKELETGCIIIKPPAPSKARNHTSHACWERPKLGHWKLNSDA 180
                               E +  C   +P AP   +       W  P  G  KLN D 
Sbjct: 227 --------------------EHKLPC---QPTAPKLFKR------WVLPMSGWAKLNVDG 286

Query: 181 AWNKDKGCGGLGWVVRDSSGSTICFGMKTIYKEWLVNLLEAK--AIAEGLNEIIHTCVRE 240
           +  ++    G+G ++R  +G+ I    +T+  +   +  EA+  A  EGL   +  C   
Sbjct: 287 SVQQNPNAAGVGMILRGDAGAIIFSACRTL--DHCTDPFEAELCACMEGLGLALEWC--- 328

Query: 241 RIPLEVETDALGVIKVINGDAS 258
           ++P+ VETD   V++   GD S
Sbjct: 347 QLPIIVETDCTAVVEA--GDRS 328

BLAST of Spg027915 vs. NCBI nr
Match: XP_035551510.1 (uncharacterized protein LOC118349853 [Juglans regia])

HSP 1 Score: 101.3 bits (251), Expect = 1.3e-17
Identity = 66/252 (26.19%), Postives = 111/252 (44.05%), Query Frame = 0

Query: 5   NDWNEDLIRQNFIPQDANDILNMPTGGKDSKDEIFWHPVKNGIFLVKSAYHLATDTISNL 64
           + WNE+L+R+ F+ ++A  I ++P     S+D++ W P K G+F VKSAY+L  +  S L
Sbjct: 601 SSWNENLVREIFLKEEAEQICSLPVSKLGSEDKLIWGPSKRGLFTVKSAYYLEQERKSRL 660

Query: 65  EASQSDERKSSSLWKSIWAMDSLPRTKVCVWKILNDIISTKTNIQSKGIDIN---PMMER 124
           +   S E+K+ S+WK+IW++      K  +WK+ ND+++T+ N+  + +  +   P+ +R
Sbjct: 661 KGECSTEKKADSIWKNIWSLRVPGVVKTFLWKVGNDVLATRKNLWRRRVIESATCPICKR 720

Query: 125 -------------PPMDIWSWMTDNLSKKELETGCI------------------------ 184
                           D+W+     + K   E  C                         
Sbjct: 721 EGETAMHILWQCPAARDVWAGSFRAIQKWTAEEDCFLAFWDKAMQKLEAEDLEIVAVAKT 780

Query: 185 -----------IIKP-----PAPSKARNHTSHACWERPKLGHWKLNSDAAWNKDKGCGGL 201
                      II P        +   N   H  W++P  G +K N DAA N      GL
Sbjct: 781 ILEDFKEAQLQIINPIEEPRQLETSGSNRGRHVLWKKPDQGGFKANWDAALNSK--FMGL 840

BLAST of Spg027915 vs. ExPASy TrEMBL
Match: B8BN96 (Reverse transcriptase domain-containing protein OS=Oryza sativa subsp. indica OX=39946 GN=OsI_37529 PE=4 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 8.0e-21
Identity = 82/280 (29.29%), Postives = 126/280 (45.00%), Query Frame = 0

Query: 3    DNNDWNEDLIRQNFIPQDANDILNMPTGGKDSKDEIFWHPVKNGIFLVKSAYHLATDTIS 62
            ++  W+   I Q F   DA  ILN+    +  +D I WHP KNG+F V+SAY LA   ++
Sbjct: 1423 EDGSWDVPKIHQYFHNLDAEVILNICISSRSEEDFIAWHPDKNGMFSVRSAYRLAAQLVN 1482

Query: 63   NLEASQSDERKSSSLWKSIWAMDSLPRTKVCVWKILNDIISTKTN--------------- 122
              E+S S     +  W+ IW      + K+  W++ ++ ++T                  
Sbjct: 1483 IEESSSSGTNNINKAWEMIWKCKVPQKVKIFAWRVASNCLATMAMFLMTLWRNWYVRNEL 1542

Query: 123  IQSKGIDINPMMERPPMDIWSWM-----------TDNLSKKELETGCIIIKPPAPSKARN 182
            I  K     P  E     I S++            D +  K +     +   P      N
Sbjct: 1543 IHGKSA---PPTETSQRFIQSYVDLLFQIRQAPQADLVKGKHVVRTVPLKGGPKYRVLNN 1602

Query: 183  HTSHACWERPKLGHWKLNSDAAWNKDKGCGGLGWVVRDSSGSTICFGMKTIYKEWLVNLL 242
            H    CWERPK G  KLN D +++   G GGLG ++R+S+G  I    K +  E   N L
Sbjct: 1603 H--QPCWERPKDGWMKLNVDGSFDASSGKGGLGMILRNSAGDIIFTSCKPL--ERCNNPL 1662

Query: 243  EA--KAIAEGLNEIIHTCVRERIPLEVETDALGVIKVING 255
            E+  +A  EGL   IH  +   +P++VETD   V++++ G
Sbjct: 1663 ESELRACVEGLKLAIHWTL---LPIQVETDCASVVQLLQG 1692

BLAST of Spg027915 vs. ExPASy TrEMBL
Match: A0A803NGM9 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 1.0e-20
Identity = 71/232 (30.60%), Postives = 108/232 (46.55%), Query Frame = 0

Query: 4    NNDWNEDLIRQNFIPQDANDILNMPTGGKDSKDEIFWHPVKNGIFLVKSAYHLATDTISN 63
            +  W+E+ +R  F  +DA  IL+MP+ G D +D+I WH  KNG + VKS Y +AT   S 
Sbjct: 1149 DGSWDENFVRSVFNMEDAELILSMPSTGWDIEDKIMWHYSKNGEYTVKSGYKMATSLAS- 1208

Query: 64   LEASQSDERKSSSLWKSIWAMDSLPRTKVCVWKILNDIISTKTNIQSKGIDINPMMER-- 123
             E  QSD++     WK++W +   P+ K  VWK++ + I T  N+  +G+D++ +  R  
Sbjct: 1209 -EQYQSDDQMCVDWWKALWRLKIPPKIKHFVWKLVYNWIPTNANLAKRGVDVDNICVRCS 1268

Query: 124  ---------------PPMDIWS----WMTDNLSKKELETGCIIIKPPAPSKARNHTSHAC 183
                              +IW+    W+   L + E   G            R    H C
Sbjct: 1269 GHVVETAAHALWECKRSKEIWALGTWWIGVGLIRFEFWGGV----------DRESLLHDC 1328

Query: 184  --WERPKLGHWKLNSDAAWNKDKGCGGLGWVVRDSSGSTICFGMKTIYKEWL 213
              W  P+ GH K+N DA      G  GL  VVRD  G  I   + ++ + W+
Sbjct: 1329 VKWAAPEFGHMKVNVDAGVKGGGGLSGLRCVVRDQGGGFIMPLLLSLIESWI 1368

BLAST of Spg027915 vs. ExPASy TrEMBL
Match: A0A6J5XPQ2 (RNase H domain-containing protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCUS35944 PE=4 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 1.4e-20
Identity = 77/300 (25.67%), Postives = 129/300 (43.00%), Query Frame = 0

Query: 7   WNEDLIRQNFIPQDANDILNMPTGGKDSKDEIFWHPVKNGIFLVKSAYHLATDTISNLE- 66
           W ED+I + F+P +A  IL++P       D++ WH   +G + V+S+Y LA     N E 
Sbjct: 13  WKEDVINRCFVPDEARCILSIPINKWGCPDKLIWHFSMHGQYTVRSSYELALHLQRNGEL 72

Query: 67  -----ASQSDERKSSSLWKSIWAMDSLPRTKVCVWKILNDIISTKTNIQSK--GIDINPM 126
                +  S     SS+WKS W  D+ P+ K  +W+  ++I++ + N++ +   +D+N +
Sbjct: 73  GHKGASEASGSYSQSSVWKSFWHADAPPKLKAFIWRGCSNILAVRHNLRRRPLQLDVNNL 132

Query: 127 MERPPMDIWSWMTDNLSKKELETGCI---------------------------------- 186
                +  W ++   ++  E     I                                  
Sbjct: 133 AAEDFISTWRYIMTMINSSEHAAEAIQWFVFGLWRIWKARNSVVFDGVQADPAEAVHLLL 192

Query: 187 -------IIK---PPAPSKARNHTSHACWERPKLGHWKLNSDAAWNKDKGCGGLGWVVRD 246
                  I K    P P +  +      W +P  G  K+N DAAW+  + CGG+GWV+RD
Sbjct: 193 QQVSEYRIAKQNLQPQPLQPASSPVPQSWCKPPPGFLKVNCDAAWSAQRLCGGVGWVIRD 252

Query: 247 SSGSTICFGMKTIYKEWLVNLLEAKAIAEGLNEIIHTCVRERIPLEVETDALGVIKVING 255
           S G  +C G +         ++E  AI   L+E  H  +   I   VE+D+   I ++NG
Sbjct: 253 SFGLLLCAGGQGDLHGSSALMMELLAIRHALSECSHFHLTNVI---VESDSQMGISMLNG 309

BLAST of Spg027915 vs. ExPASy TrEMBL
Match: A0A6J5UV01 (Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS32434 PE=4 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 2.0e-19
Identity = 86/297 (28.96%), Postives = 128/297 (43.10%), Query Frame = 0

Query: 7    WNEDLIRQNFIPQDANDILNMPTGGKDSKDEIFWHPVKNGIFLVKSAYHLATDTISNLEA 66
            W+ DL+ + F P +A  I  +P       D+  WH   +G + V+S Y +A     N E 
Sbjct: 859  WDMDLVNRCFNPNEAKCISTIPISRWGCPDKKVWHYTSHGGYTVRSGYAVALTLRRNGEL 918

Query: 67   SQSDERKSSS------LWKSIWAMDSLPRTKVCVWKILNDIISTKTNIQSKGIDINPM-- 126
             +  E +SS       +WKSIW +   P+ +  +WK   ++++ K N+   GID+     
Sbjct: 919  GRKAEGESSQGDKQRRIWKSIWGLQVPPKIRTFLWKCCRNVLAVKENLLHWGIDVETTCA 978

Query: 127  ---MERPPMD-IWSWMTDNLSKK------------------------------ELETGCI 186
                E    D I  W  +NL KK                              E  T  +
Sbjct: 979  LCGQEGESQDFIVCW--ENLLKKYEQVEHCNDILQGTVIEPHVAVECFLNQVHEFRTAQM 1038

Query: 187  IIKP-----PAPSKARNHTSHACWERPKLGHWKLNSDAAWNKDKGCGGLGWVVRDSSGST 246
              KP     P P + +  T    W +   G  K+N DAAW      GG+GWVVRDS G  
Sbjct: 1039 HGKPLINELPRPPEIQAETV-TSWRKLPYGVLKVNCDAAWVSQTQMGGVGWVVRDSYGWM 1098

Query: 247  ICFGMKTIYKEWLVNLLEAKAIAEGLNEIIHTCVRERI-PLEVETDALGVIKVINGD 256
            +  G K   +      +EAKAI E L      CV+  +  LEVE+D+L +I+++ G+
Sbjct: 1099 VQAGCKGDMRGGSALAMEAKAIREAL----LACVQSGLSKLEVESDSLQLIRMLGGE 1148

BLAST of Spg027915 vs. ExPASy TrEMBL
Match: A0A2K2DVE7 (Uncharacterized protein OS=Brachypodium distachyon OX=15368 GN=BRADI_1g76105v3 PE=4 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 4.9e-18
Identity = 72/262 (27.48%), Postives = 122/262 (46.56%), Query Frame = 0

Query: 1   MDDNNDWNEDLIRQNFIPQDANDILNMPTGGKDSKDEIFWHPVKNGIFLVKSAYHLATDT 60
           +D+N  W ++L+R+ F+P D++ IL + T  + + D + WHP K G F V+SAY L  D+
Sbjct: 107 LDENGGWRQELVRRIFLPADSDAILRIKTSPRLAPDFLAWHPEKMGSFSVRSAYRLGVDS 166

Query: 61  ---ISNLEASQSDERKSSSLWKSIWAMDSLPRTKVCVWKILNDIISTKTNIQSKGIDINP 120
               ++  A+ S    S   W  IW   + P+ K+  WK  N+ ++T+ N + +G+    
Sbjct: 167 SKLTNDRTATSSHPDGSDPCWNKIWTCIAPPKVKIFAWKAANNALATEENKKRRGM---- 226

Query: 121 MMERPPMDIWSWMTDNLSKKELETGCIIIKPPAPSKARNHTSHACWERPKLGHWKLNSDA 180
                               E +  C   +P AP   +       W  P  G  KLN D 
Sbjct: 227 --------------------EHKLPC---QPTAPKLFKR------WVLPMSGWAKLNVDG 286

Query: 181 AWNKDKGCGGLGWVVRDSSGSTICFGMKTIYKEWLVNLLEAK--AIAEGLNEIIHTCVRE 240
           +  ++    G+G ++R  +G+ I    +T+  +   +  EA+  A  EGL   +  C   
Sbjct: 287 SVQQNPNAAGVGMILRGDAGAIIFSACRTL--DHCTDPFEAELCACMEGLGLALEWC--- 328

Query: 241 RIPLEVETDALGVIKVINGDAS 258
           ++P+ VETD   V++   GD S
Sbjct: 347 QLPIIVETDCTAVVEA--GDRS 328

BLAST of Spg027915 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 52.0 bits (123), Expect = 8.5e-07
Identity = 43/163 (26.38%), Postives = 73/163 (44.79%), Query Frame = 0

Query: 94  VWKILNDIISTKTNIQSKGIDINPMMERPPMDIWSWMTDNLSKKELETGCIIIKPPAPSK 153
           +WK  N+++      + K  D   ++ R   D   W T    ++ELE      K   P  
Sbjct: 84  LWKSRNELM-----FKGKEYDAPEVLRRAMEDFEEWST----RRELEG-----KASGPQV 143

Query: 154 ARNHTSHACWERPKLGHWKLNSDAAWNKDKGCGGLGWVVRDSSGSTICFGMKTIYKEWLV 213
            RN +    W+ P     K N+DA W  +    G+GW++R+ SG  +  G + + +    
Sbjct: 144 ERNLSVQ--WKAPPYQWVKCNTDATWQLENPRCGIGWILRNESGGVLWMGARALPR--TK 203

Query: 214 NLLEAKAIAEGLNEIIHTCVRERIP-LEVETDALGVIKVINGD 256
           N+LEA+   E L   + T  R     +  E+DA  ++ ++N D
Sbjct: 204 NVLEAE--LEALRWAVLTMSRFNYKRIIFESDAQALVNLLNSD 226

BLAST of Spg027915 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 47.4 bits (111), Expect = 2.1e-05
Identity = 33/110 (30.00%), Postives = 56/110 (50.91%), Query Frame = 0

Query: 156 NHTSHACWERPKLGHWKLNSDAAWNKDKGCGGLGWVVRDSSGSTICFGMKTIYKEWLVNL 215
           N +S   W  P     K N+DA WN+D    G+GWV+R+  G     G + + K  L ++
Sbjct: 413 NRSSCGRWRPPPHQWVKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARALPK--LKSV 472

Query: 216 LEAKAIAEGLNEIIHTCVRERIPLEV-ETDALGVIKVINGDA--SSISPT 263
           LEA+   E +   + +  R +    + E+D+  +I+++N D    S+ PT
Sbjct: 473 LEAE--LEAMRWAVLSLSRFQYNYVIFESDSQVLIEILNNDEIWPSLKPT 518

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
EEC68887.11.7e-2029.29hypothetical protein OsI_37529 [Oryza sativa Indica Group][more]
CAB4313148.12.8e-2025.67unnamed protein product [Prunus armeniaca][more]
CAB4279811.14.1e-1928.96unnamed protein product [Prunus armeniaca][more]
PNT78255.11.0e-1727.48hypothetical protein BRADI_1g76105v3 [Brachypodium distachyon][more]
XP_035551510.11.3e-1726.19uncharacterized protein LOC118349853 [Juglans regia][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
B8BN968.0e-2129.29Reverse transcriptase domain-containing protein OS=Oryza sativa subsp. indica OX... [more]
A0A803NGM91.0e-2030.60Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A6J5XPQ21.4e-2025.67RNase H domain-containing protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCU... [more]
A0A6J5UV012.0e-1928.96Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=... [more]
A0A2K2DVE74.9e-1827.48Uncharacterized protein OS=Brachypodium distachyon OX=15368 GN=BRADI_1g76105v3 P... [more]
Match NameE-valueIdentityDescription
AT2G34320.18.5e-0726.38Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT4G29090.12.1e-0530.00Ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 169..260
e-value: 9.1E-7
score: 31.0
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 174..257
e-value: 1.8E-9
score: 37.5
NoneNo IPR availablePANTHERPTHR46736FAMILY NOT NAMEDcoord: 10..250
NoneNo IPR availablePANTHERPTHR46736:SF6SUBFAMILY NOT NAMEDcoord: 10..250
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 170..254

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg027915.1Spg027915.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity