Spg027529 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg027529
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionLeucyl-tRNA synthetase
Locationscaffold11: 4174781 .. 4183053 (+)
RNA-Seq ExpressionSpg027529
SyntenySpg027529
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGAAATGGAAAACCCTACTTGTATATGTGCTCCACGCCATTTCTTCTTCTTCTTCATCTAGCCGCTGCCTCTCTCCCGTTCACTACTGCCGCCGGCGCCGTCTTTCTTCCGCTCGTGCCGCCGCAGCCTCCCTCCTAGTGGTTTTGCACTGAATTTCTACTCCCTTGTTGTTACTGGTATTTCCCAATCTTCATCTATGTCTTCCTTCTCACTTGAAAAACGAAAATTAGAACTTGTCTAATGAAATAATGGAAGAGTTGTTTGGTTTTTATTTTTTTAAAATAACAATAGCCGATTGCAACTGAAAAAGAAACAGCCTTGTGGCAAATTGGGACGTGGTTTGTGTTTGGCTGTTCTGATTCGGACATGAATCAGTCATCCAATTCTCTTTTAATGCCTAGCTTGATTGATTGAACGAACAACTTCAACCTCGTTTATTTTTGTAGATTATAGATTATAGTTGGGAGGGGACAAAGGGACAGAGAAATGGGATTGGGTAATAGGAATGGTAGAATGTTTGACCGTGGACACATGAGGGGTGAATGTTGAAGCAAAAGGAAATCGAGACTAATTAAAAATGATGGGAACGTAAACACGATTGGATGAAGGCTCAAGACATAAGTATCCTTCATCATGAATACTATAGCCAAGAAAAACACATTCAATAGAACAAAACAATAGCTTTATGAGGATTAAAAGGACTAAGAAAAGGAAAACAAGCACAACCAAAGGAACAGACACAAAAGTTTATAATTAGGGGCATGACCATAAAGCTTTTTGTATGGGCTAATAAGATTAAAAGCAACAATAGGAAGTCTATTAATTGAATGAACTATAGTTTGAAAAGCATAAGTCCAAAAAGTAAGAGGATATGGGAGTGAGCAAGGAGAGAAAGACCAACCTTGACAACATGGCGATTCTTCCTCTCAACTTTACCTTTTTTGTGGTGCATGAGGGCAAGATATTCAATATTTAATGCCATTTGAAGCTGAAAAGGAACAAATGGAGTGAAATTCACCACCCCAATCAGTTTGGAAAGTAAGGATAGAGAAATTAAATTGGTTGGAAACCATTGTTTTAAAATGCATGAAGGCACAAGAGTTTCATCTTTTGTTTTAAGTATAGCCAAATGAGTCGTGTATAATCATCAACAAATAGAACAAAAAGTTGTGCACCATCGAAGGAAGAAACAAAATCAGGGCCCCAATAACCTTGCTTTATAATGGAAGAGGTTGGATGTCCTAATCTATGGTGCCAAATAAAAAGATCTTGGAAAAGTTGAGAGGAGACCTATACTTGCTAACAATAACCTTGTACCACAAGTCGTTGGTTCATGATGGAATCGACAAAGCCATTTAGCTAGTGGGGCTTTTATCCTATCCCTAACATTATTGATACTTAACCCCACCCCACCCAAAAAAAAAAATCTAGTGGACTAGAAACAACTTCCCAATTCACAAGGTGCAAGCCTTTGCCTTCATCAACTCCCTCCCGCATATACTTCTCAACCACCTTGCTTATTGAATAGGGGACTCTAAAAAGGGACATATAATAGATTGAATTAGGGTAAGTCTCCCCCTTTTGAGAAAAAACTCTCTCCAAGAGGCAAGTCTTTTCCTAATCTTAATGGTGAACAAATCCCAAAAGGAGATACTCTTGGGATTATGACCTAAGGGTAATCCTAAACAGGAGGAAGGAAAGGAACCCACTTCACACCCCACCCAAGAAACCCACCTCTTTAAGCTTAGCCTGGTCACAGTTAACACCTAGAATTTGACTATTGTCTCTGTTAATCCTCAAACCCGATATGGCCTCAGAAAACGAGAGGATAAGATTCAAATTACGAAAGGATTCCTCCTAACCAGAGCAGAAAAACATAGTATCATCAGCAAACTGGAGATGAGATAGAACCACCTTCAAGTTGTCTCCGAAGGAGGACGGTCTTAGTTGCAATGTCCTTCACAAAAAAAATGAGATAGATAAAAACTCAACAAGGGCATGATTATCATGGCATAATTTAGTAATGCTAATAAGATGTTTTGTATTTGGAGGGAGATGCAAAGCAAATTTTAAACGCAGGCAGTTTCTTCCTTAGTGTGCCTTTTTATATTTTTATATATACTATAGATATAGAGTTAGCTTAAAAGAGAGTTGATTATCTTATTACTATTTTGTATTTGCATCATTCAAGTATTTATTCTTCTCCGTTATGCTTTTTAAGCCTATTTTGTAATATATATCTCATCTCATTCTCAAAAGCATTTGATTTACAGGGCAAGCATACTCTCTCTCTCGTTGGTTTAATAATTTACTAGTGTCTCACTGGCTTTTTCTCATTCCTTGAAGAGCTTTTTGCCATTTTCGACAAATCACCGTTCTGTTCTGAATATAAGGTATAGACGTGACTTTAGTCTGCTTTCATCACTCTCCTTTAGACCTCAAATCTGGGAGTTTGTTAAGGTTCTGTTCTAATACTACCTTGTGTGTGTGTGTTATTTTCTTTTCTATTGGTGCGTGCATGTTTTTTTTTTATTTTATTTTATTTGGGGGGGGGGGGGGGTCATTGGGTGAATGTGGGTTTGAGCTTCATTTATGTTGCATTTAATTGTCGAGGGAATGGCCCAGTTCATGGATTGAATTCATTGTTAATAGCTACGTAGGCCAATTAGCATATTGTTAGGAACCCGAATTCTGAAAACACACTTTGATATATATTGCAATATCAAGAGAAACTACAATATACTAATAGCCTTTCGAGAGGGCTAATTCTCTCCCAAGAGTCCCACAATGGACACTGCCACTAAAAATTCTCCACCCCTTCAAAGACTCCCTAACTTCCCTATTTATAACCAAGACTCCTTTACTCATTACTAATATGCCCTCACTCTTAATCTAATACGAATCTCCCAAATATACCATACTAGTATCCTCACACATATTTAGGTATTTGGTTCACTTTCAGTCCTGTTTTGATTTGTGAGTATCAAATGAATTATTTATTTTATTTTCTGTACGAGAGATTTTTTCAAGATTAAATGCTGTTTTAGCTGGCTAAAACCAGCGTGGAGTAGATAAAATTCCTACTTTGCTGTGGGATTGTGTCATATATTATTTTAAAATATAATTTGATGTTTGGTCAAGTCATCAATAATTTGTTGGGGTATCAAGTTTGATTTATTTAGTCTATTGTGGTATAGTACACGAGTGATTTTTTAGGCTGGACTTCATGTGCACTCCTAAGATTTACGTTCTTTGTGTCTGGTGGTTTATGCATTTGTCCCTGCATGTTTCAAGATTGTAGGAGCTTGGGGATTTGTACCTATTTTACTTTTCAATCTAAATAATTAGGGTAAATTACAATTTTGGCCCAAGAGTCTTTGGTTGGGGAGTTCTCGGACCAAAGGCAAAAACTCAAGGACTAAAGGTGTTATTTAGCCTAATAATTATAATGGTTCAGAGTCTTTGCAACATTTTTGCAACATTTATTCTTTGATTGCTTATATTCTCATCGGTGTGAGGAGAGGTTATGCCTATTTTCAAGCTTTATTGGGTTCTCGAATATTAGTCATTTAAAGGAAATGTGTTGCAGCTATTGATTGGTCCATCTCTTATGCCTACTTCTAAATTGTTGTGGTCAAATGTAGTAAAAGTTGTTTTGTTAGAGATTTGGTTCGAAAGGAATCAGAGGGTTTTCTATGATAAATCCCTCCCTTGGAAGGATCGTTTTGGTTCTGCTCGTCTCAAAGCTTTGTCTTGGTGTTTTCTGTCCAAGCTTTTTTTAGGCTACTACATTCAAGATATTTTCCTTAATTGGAATGTTTTTATTTTTCCATCGTACTTTATGTTTTTCATTTGAATTTGTTTTTAACTTGCAGTGCTTTAATCTTTATTTTTTGTCTCGTTGTATTTTGAGCATTAGTCTCTTTTCATTATTTCAATGAAAAGTGTGGTTTCCTTTTCAAAAACAATTATAATGGTTGAGAGTGATGGTTTAGAAGCAAAGTCCTCTGAATCTATTGAAGATTAATTAATTTTTAAAGGATACTTTTGTTTATATATCCATATATTTGTTAAATAGAGTTCCTTAAATTAGGATCGAAGTTGAATGGATTTGAGTTCTAATATGCACCCTGTCTGTCATTCTTCTAGATGAGAGTGCTTTTTGAAATATGTGTTACGTATGATATACACAGTCTTAAATGACGCCTGAAAGTTCATTTATTAGTTGAATTATTGGAACTTGACCTACTTGCATACCGTGTAAACTTTTTCTGTATGCCTTAAATTTTTAGAGTGCTTCCTATGGTGTACCTCTTATGATGTTGTTGATATCTGCAGATTTTTCAGTCTTACAGAAATGGCATCAGAGAGTGGGAAAAGCTTTGCTAGGAGGGACAGCCTCCGAGAAATTGAAGCGAAGGTTCGAGTTTTGTGGGAAGAGAATGATGTTTTTAGGGCAGAGGCATGTGAAACACCTCCTAAAGTGGGCGAGAAGTTCTTTGGAAATTTTCCCTTTCCTTATATGAATGGATTTTTGCATCTTGGTCATGCTTTCTCCCTTTCAAAATTGGAGTTTGCCGCAGCATATCACAGGTTAAGAGGTGCAAATGTCCTATTACCATTTGGTTTCCACTGCACAGGAATGCCTATCAAGGCCTCTGCTGATAAGCTTGCTCGGGAAATCCAACAGTTTGGTGATCCACCTGTGTTCCCTCGGGAAATAGAAGAGCAAGAAAACTTGAAAGCAGAAGCTGAGGATGCAAATGAAAGTAATCCAACATTGCCTGACAAATTCAAGGGCAAGAAATCTAAGGCAGCTTCAAAGTCAAGTGGACAGATGTACCAGTGGGAAATAATGCGCAGTTTTGGTCTGTCTGATAGTGAGATATCCAAGTTTCAAAACCCTTATAACTGGTTGACATTCTTTCCTCCGTTGGCTATGGAGGACCTTAAGGCTTTTGGTTTAGGTTGTGATTGGAGAAGATCTTTTATTACTACTGATATGAATCCATTTTTTGATTCATTTGTACAATGGCAAATGCGAAAGTTGAAATCCATGGGGAAGATTGTGAAAGATGTCAGATATACTATATATTCGCCCTTAGATGGCCAACCCTGTGCTGATCATGATAGGGCAACCGGTGAAGGGGTTCAACCACAGGATTACACTCTGATTAAGATGGAGGTTGTGCCACCTTTTCCTCCTAAAATGGGAGTGTTGGTGGGGAGAAAAGTGTATTTAGCTGCTGCAACATTGAGACCCGAGACTATGTATGGTCAAACAAATGCATGGGTATTACCAGAGGGTAAATATGGAGCATTTGAAATTAACGATACTGATGTATTTATTATTACAGAACGAGCAGCCCTTAACTTAGCCTATCAGAAATTTTCTAAGGTTCCTGAAAAACCTACTTGCTTGATTCAGCTGACTGGTAATGATTTGATAGGCCTCCCATTGAAGTCCCCTCTAGCCTTCAATGAGATCATTTATGCTCTTCCCATGCTTACAATCCTTACGGATAAGGGAACTGGTATTGTGACCAGTGTACCTAGTGATGCTCCTGATGATTATATGGCCATGCATGATTTAAAATCAAAACCAGCTCTTAGGGCCAAGTATGGTGTTAAAGATGAATGGGTGCTTCCCTATGATATTGTTCCCATTATTGATATCCCAGAATTTGGAGACAGGGCTGCTGAAAAAGTTTGCTTGGATCTAAAAATCAAAAGTCAGAATGAGAAGGACAAGCTTGCTGAAGCTAAAAGATTGACGTATTTGAAAGGATTTACTGATGGAACACTAATTGTTGGTGAATTTGCTGGAAGGAAAGTGCAGGAAGCAAAGCCTTTGATTCGGAGCAAGCTAATTGAAACTGGCCAAGCTATCCCATATAGTGAGCCTGAGAAGCGAGTTATCTCCCGGTCTGGAGATGAGTGCATTGTAGCTCTTACTGATCAATGGTGACTGTCCCTAACCAACTCTTTAGTTAATAACTAAACATATCATTGATTGATCGAATAATATTTCTAATCTCTATTCTAATGGTATTCCTAACAGGTACATCACGTATGGGGAATCAGAGTGGAAGAAGTTGGCTGAAGAGTGCTTGGCCAACATGCAACTGTTTTCTGATGAGACAAGGCATGGATTTGAGCACACACTGAGTTGGCTTAACCAGTGGGCGTGCTCACGAAGTTTTGGCCTTGGAACGCGTATCCCTTGGGATGAACAATTTCTAGTTGAGTCATTATCTGATTCCACCATTTACATGGCTTACTACACCATTGCTCATTTGTTGCATAATGGGGACTTGTATGGATCAGGTGCCTCTGCAATAAAACCCGAGCAGATGACTGATGAAGTGTGGGATTTTGTCTTTTGTGGTGCTGCGGAACCAAAATCAACTGGTATCTCAAAGTCAATTCTTAATAAGATGAAACAGGAGTTTGAGTATTGGTATCCATTTGATCTTAGGGTTTCTGGAAAAGATCTTATTCAGAATCACTTGACATTCTGTATTTACAATCATACTGCAATTATGCCAAGGCACCATTGGCCACGTGGGTTTAGATGTAATGGGCACATCATGCTTAATTCTGAAAAGATGTCCAAGTCTACTGGGAATTTTAGAACTCTGCGTCAGGCAATTGAAGAGTTTTCTGCTGATGCCACACGATTCTCATTGGCTGATGCTGGTGATGGTGTTGATGATGCAAATTTTGTATTTGAGACTGCAAATGCTGCAATACTACGTTTGACTAAAGAAATTGCATGGATGGAAGATATTCTACAGGCTGATTCTGCTTCATTCCTTAGAACAGGCCCTCCATCAACCTATGCTGACCGTGTCTTTGAAAATGAAATCAATATAGCTGTCAAAATGACTGAGCAAAATTACAAAGATTACATGTTTCGGGAAGCTTTGAAGACCGGTTTTTATGATCTCCAAGCAGCTAGGGATGAGTATAGGTTTTCATGTGGTGCCGCGGGCATGAATCGTGATTTGGTTTTCCGTTTTATGGATGTCCAGACGCGACTTATTACTCCAATCTGTCCGCACTATGCAGAATATGTTTGGAGGGAAATGTTAAAGAAGGAAGGATTTGTTGTTAATGCTGGCTGGCCTTTGGCTGATTCTCCTGATTTAACTCTCAAAAGTGCAAACAAGTATCTGCAAGACTCAATAGTTTTGATGAGGAAGCTTCTTCAAAAGCAACTTTTGGGATCCAAAAAAGGCAACAAGAAGGGCGCTCCCGTCACAACGGTGATTGAGGACAAGAAACTGACTGGCTTGATATATGTGAACGAACAATTTGACGGATGGAAAGCAGAATGCCTAAGGATACTCCGAAGTAAATTTGACTCGACTAAACGTACATTTGCACCAGACAGCGAAATAATGGAAGCACTACAGAAGAGTTCAGTTGGGCAGGCAGTGGATTTTAGACAAACACAGAAGTTGTGTATGCCATTCTTGAGGTTTAAGAAGGATGAGGCTGCTGCACTCGGGGTTCATGCCTTGGACTTGAAGCTTCCTTTTGGGGAGATGGAGGTCCTTAAAGAGAACTTGGAGTTGATTAAGAGGCAAATAGGTTTGGAAGAGGTGGAGGTTTTGTGTGCATCCGACACCGATGCTCTTACAAAAGCCGGTGCTTTGGCTTCACTGTTGAAGCAGAATCCTCCATCCCCAGGGAATCCTACTGCTATTTTCTTGACAAGTTGAGAAAAATATTCATGGCATTTTTGGTGAGTGGTCAATTGTCTTTCTAGCTGTATACTCAATTGTTTTATTAGTCTTGCTATTTCTTTTTCAATCTACACGTTTTTTATTGAATTCAAAATTTCTTATCTTATAAAGAGAGAATTTCCTTTGGGAAAAAGAATCATTTTTAAATACATGGGTGTAGTTTTCTTTAAAGTTGTAAATTAAACAAATTGAATTATGAACTTGACTAAACGAGTAACGCTGCATATACAATTTTCAAGATGGTTAGGATGTTGTAATCGTGAAGACAATTCTAACCATTTGTTTTCGCTATGATGAAGGGAGATGAGGCCTCATTACTCAATGTGGTACAGAAAGAAGCGTTATGAGTAGTACGTTGCCAATGGCATCTCTGTGCCAATTCGGTAATTCTTTTAGGCCTAGCTTTTTCATACTATGCACTTATATCTTTTTTGTATTCTACATATTTTCAATCCGTGCATCTACTGTTGGGCCAATTTTTTTTTTTTTTTAAATATTGCACTTTTCACGGTTTTTTTTTC

mRNA sequence

ATGGCATCAGAGAGTGGGAAAAGCTTTGCTAGGAGGGACAGCCTCCGAGAAATTGAAGCGAAGGTTCGAGTTTTGTGGGAAGAGAATGATGTTTTTAGGGCAGAGGCATGTGAAACACCTCCTAAAGTGGGCGAGAAGTTCTTTGGAAATTTTCCCTTTCCTTATATGAATGGATTTTTGCATCTTGGTCATGCTTTCTCCCTTTCAAAATTGGAGTTTGCCGCAGCATATCACAGGTTAAGAGGTGCAAATGTCCTATTACCATTTGGTTTCCACTGCACAGGAATGCCTATCAAGGCCTCTGCTGATAAGCTTGCTCGGGAAATCCAACAGTTTGGTGATCCACCTGTGTTCCCTCGGGAAATAGAAGAGCAAGAAAACTTGAAAGCAGAAGCTGAGGATGCAAATGAAAGTAATCCAACATTGCCTGACAAATTCAAGGGCAAGAAATCTAAGGCAGCTTCAAAGTCAAGTGGACAGATGTACCAGTGGGAAATAATGCGCAGTTTTGGTCTGTCTGATAGTGAGATATCCAAGTTTCAAAACCCTTATAACTGGTTGACATTCTTTCCTCCGTTGGCTATGGAGGACCTTAAGGCTTTTGGTTTAGGTTGTGATTGGAGAAGATCTTTTATTACTACTGATATGAATCCATTTTTTGATTCATTTGTACAATGGCAAATGCGAAAGTTGAAATCCATGGGGAAGATTGTGAAAGATGTCAGATATACTATATATTCGCCCTTAGATGGCCAACCCTGTGCTGATCATGATAGGGCAACCGGTGAAGGGGTTCAACCACAGGATTACACTCTGATTAAGATGGAGGTTGTGCCACCTTTTCCTCCTAAAATGGGAGTGTTGGTGGGGAGAAAAGTGTATTTAGCTGCTGCAACATTGAGACCCGAGACTATGTATGGTCAAACAAATGCATGGGTATTACCAGAGGGTAAATATGGAGCATTTGAAATTAACGATACTGATGTATTTATTATTACAGAACGAGCAGCCCTTAACTTAGCCTATCAGAAATTTTCTAAGGTTCCTGAAAAACCTACTTGCTTGATTCAGCTGACTGGTAATGATTTGATAGGCCTCCCATTGAAGTCCCCTCTAGCCTTCAATGAGATCATTTATGCTCTTCCCATGCTTACAATCCTTACGGATAAGGGAACTGGTATTGTGACCAGTGTACCTAGTGATGCTCCTGATGATTATATGGCCATGCATGATTTAAAATCAAAACCAGCTCTTAGGGCCAAGTATGGTGTTAAAGATGAATGGGTGCTTCCCTATGATATTGTTCCCATTATTGATATCCCAGAATTTGGAGACAGGGCTGCTGAAAAAGTTTGCTTGGATCTAAAAATCAAAAGTCAGAATGAGAAGGACAAGCTTGCTGAAGCTAAAAGATTGACGTATTTGAAAGGATTTACTGATGGAACACTAATTGTTGGTGAATTTGCTGGAAGGAAAGTGCAGGAAGCAAAGCCTTTGATTCGGAGCAAGCTAATTGAAACTGGCCAAGCTATCCCATATAGTGAGCCTGAGAAGCGAGTTATCTCCCGGTCTGGAGATGAGTGCATTGTAGCTCTTACTGATCAATGGTACATCACGTATGGGGAATCAGAGTGGAAGAAGTTGGCTGAAGAGTGCTTGGCCAACATGCAACTGTTTTCTGATGAGACAAGGCATGGATTTGAGCACACACTGAGTTGGCTTAACCAGTGGGCGTGCTCACGAAGTTTTGGCCTTGGAACGCGTATCCCTTGGGATGAACAATTTCTAGTTGAGTCATTATCTGATTCCACCATTTACATGGCTTACTACACCATTGCTCATTTGTTGCATAATGGGGACTTGTATGGATCAGGTGCCTCTGCAATAAAACCCGAGCAGATGACTGATGAAGTGTGGGATTTTGTCTTTTGTGGTGCTGCGGAACCAAAATCAACTGGTATCTCAAAGTCAATTCTTAATAAGATGAAACAGGAGTTTGAGTATTGGTATCCATTTGATCTTAGGGTTTCTGGAAAAGATCTTATTCAGAATCACTTGACATTCTGTATTTACAATCATACTGCAATTATGCCAAGGCACCATTGGCCACGTGGGTTTAGATGTAATGGGCACATCATGCTTAATTCTGAAAAGATGTCCAAGTCTACTGGGAATTTTAGAACTCTGCGTCAGGCAATTGAAGAGTTTTCTGCTGATGCCACACGATTCTCATTGGCTGATGCTGGTGATGGTGTTGATGATGCAAATTTTGTATTTGAGACTGCAAATGCTGCAATACTACGTTTGACTAAAGAAATTGCATGGATGGAAGATATTCTACAGGCTGATTCTGCTTCATTCCTTAGAACAGGCCCTCCATCAACCTATGCTGACCGTGTCTTTGAAAATGAAATCAATATAGCTGTCAAAATGACTGAGCAAAATTACAAAGATTACATGTTTCGGGAAGCTTTGAAGACCGGTTTTTATGATCTCCAAGCAGCTAGGGATGAGTATAGGTTTTCATGTGGTGCCGCGGGCATGAATCGTGATTTGGTTTTCCGTTTTATGGATGTCCAGACGCGACTTATTACTCCAATCTGTCCGCACTATGCAGAATATGTTTGGAGGGAAATGTTAAAGAAGGAAGGATTTGTTGTTAATGCTGGCTGGCCTTTGGCTGATTCTCCTGATTTAACTCTCAAAAGTGCAAACAAGTATCTGCAAGACTCAATAGTTTTGATGAGGAAGCTTCTTCAAAAGCAACTTTTGGGATCCAAAAAAGGCAACAAGAAGGGCGCTCCCGTCACAACGGTGATTGAGGACAAGAAACTGACTGGCTTGATATATGTGAACGAACAATTTGACGGATGGAAAGCAGAATGCCTAAGGATACTCCGAAGTAAATTTGACTCGACTAAACGTACATTTGCACCAGACAGCGAAATAATGGAAGCACTACAGAAGAGTTCAGTTGGGCAGGCAGTGGATTTTAGACAAACACAGAAGTTGTGTATGCCATTCTTGAGGTTTAAGAAGGATGAGGCTGCTGCACTCGGGGTTCATGCCTTGGACTTGAAGCTTCCTTTTGGGGAGATGGAGGTCCTTAAAGAGAACTTGGAGTTGATTAAGAGGCAAATAGGTTTGGAAGAGGTGGAGGTTTTGTGTGCATCCGACACCGATGCTCTTACAAAAGCCGGTGCTTTGGCTTCACTGTTGAAGCAGAATCCTCCATCCCCAGGGAATCCTACTGCTATTTTCTTGACAAGTTGA

Coding sequence (CDS)

ATGGCATCAGAGAGTGGGAAAAGCTTTGCTAGGAGGGACAGCCTCCGAGAAATTGAAGCGAAGGTTCGAGTTTTGTGGGAAGAGAATGATGTTTTTAGGGCAGAGGCATGTGAAACACCTCCTAAAGTGGGCGAGAAGTTCTTTGGAAATTTTCCCTTTCCTTATATGAATGGATTTTTGCATCTTGGTCATGCTTTCTCCCTTTCAAAATTGGAGTTTGCCGCAGCATATCACAGGTTAAGAGGTGCAAATGTCCTATTACCATTTGGTTTCCACTGCACAGGAATGCCTATCAAGGCCTCTGCTGATAAGCTTGCTCGGGAAATCCAACAGTTTGGTGATCCACCTGTGTTCCCTCGGGAAATAGAAGAGCAAGAAAACTTGAAAGCAGAAGCTGAGGATGCAAATGAAAGTAATCCAACATTGCCTGACAAATTCAAGGGCAAGAAATCTAAGGCAGCTTCAAAGTCAAGTGGACAGATGTACCAGTGGGAAATAATGCGCAGTTTTGGTCTGTCTGATAGTGAGATATCCAAGTTTCAAAACCCTTATAACTGGTTGACATTCTTTCCTCCGTTGGCTATGGAGGACCTTAAGGCTTTTGGTTTAGGTTGTGATTGGAGAAGATCTTTTATTACTACTGATATGAATCCATTTTTTGATTCATTTGTACAATGGCAAATGCGAAAGTTGAAATCCATGGGGAAGATTGTGAAAGATGTCAGATATACTATATATTCGCCCTTAGATGGCCAACCCTGTGCTGATCATGATAGGGCAACCGGTGAAGGGGTTCAACCACAGGATTACACTCTGATTAAGATGGAGGTTGTGCCACCTTTTCCTCCTAAAATGGGAGTGTTGGTGGGGAGAAAAGTGTATTTAGCTGCTGCAACATTGAGACCCGAGACTATGTATGGTCAAACAAATGCATGGGTATTACCAGAGGGTAAATATGGAGCATTTGAAATTAACGATACTGATGTATTTATTATTACAGAACGAGCAGCCCTTAACTTAGCCTATCAGAAATTTTCTAAGGTTCCTGAAAAACCTACTTGCTTGATTCAGCTGACTGGTAATGATTTGATAGGCCTCCCATTGAAGTCCCCTCTAGCCTTCAATGAGATCATTTATGCTCTTCCCATGCTTACAATCCTTACGGATAAGGGAACTGGTATTGTGACCAGTGTACCTAGTGATGCTCCTGATGATTATATGGCCATGCATGATTTAAAATCAAAACCAGCTCTTAGGGCCAAGTATGGTGTTAAAGATGAATGGGTGCTTCCCTATGATATTGTTCCCATTATTGATATCCCAGAATTTGGAGACAGGGCTGCTGAAAAAGTTTGCTTGGATCTAAAAATCAAAAGTCAGAATGAGAAGGACAAGCTTGCTGAAGCTAAAAGATTGACGTATTTGAAAGGATTTACTGATGGAACACTAATTGTTGGTGAATTTGCTGGAAGGAAAGTGCAGGAAGCAAAGCCTTTGATTCGGAGCAAGCTAATTGAAACTGGCCAAGCTATCCCATATAGTGAGCCTGAGAAGCGAGTTATCTCCCGGTCTGGAGATGAGTGCATTGTAGCTCTTACTGATCAATGGTACATCACGTATGGGGAATCAGAGTGGAAGAAGTTGGCTGAAGAGTGCTTGGCCAACATGCAACTGTTTTCTGATGAGACAAGGCATGGATTTGAGCACACACTGAGTTGGCTTAACCAGTGGGCGTGCTCACGAAGTTTTGGCCTTGGAACGCGTATCCCTTGGGATGAACAATTTCTAGTTGAGTCATTATCTGATTCCACCATTTACATGGCTTACTACACCATTGCTCATTTGTTGCATAATGGGGACTTGTATGGATCAGGTGCCTCTGCAATAAAACCCGAGCAGATGACTGATGAAGTGTGGGATTTTGTCTTTTGTGGTGCTGCGGAACCAAAATCAACTGGTATCTCAAAGTCAATTCTTAATAAGATGAAACAGGAGTTTGAGTATTGGTATCCATTTGATCTTAGGGTTTCTGGAAAAGATCTTATTCAGAATCACTTGACATTCTGTATTTACAATCATACTGCAATTATGCCAAGGCACCATTGGCCACGTGGGTTTAGATGTAATGGGCACATCATGCTTAATTCTGAAAAGATGTCCAAGTCTACTGGGAATTTTAGAACTCTGCGTCAGGCAATTGAAGAGTTTTCTGCTGATGCCACACGATTCTCATTGGCTGATGCTGGTGATGGTGTTGATGATGCAAATTTTGTATTTGAGACTGCAAATGCTGCAATACTACGTTTGACTAAAGAAATTGCATGGATGGAAGATATTCTACAGGCTGATTCTGCTTCATTCCTTAGAACAGGCCCTCCATCAACCTATGCTGACCGTGTCTTTGAAAATGAAATCAATATAGCTGTCAAAATGACTGAGCAAAATTACAAAGATTACATGTTTCGGGAAGCTTTGAAGACCGGTTTTTATGATCTCCAAGCAGCTAGGGATGAGTATAGGTTTTCATGTGGTGCCGCGGGCATGAATCGTGATTTGGTTTTCCGTTTTATGGATGTCCAGACGCGACTTATTACTCCAATCTGTCCGCACTATGCAGAATATGTTTGGAGGGAAATGTTAAAGAAGGAAGGATTTGTTGTTAATGCTGGCTGGCCTTTGGCTGATTCTCCTGATTTAACTCTCAAAAGTGCAAACAAGTATCTGCAAGACTCAATAGTTTTGATGAGGAAGCTTCTTCAAAAGCAACTTTTGGGATCCAAAAAAGGCAACAAGAAGGGCGCTCCCGTCACAACGGTGATTGAGGACAAGAAACTGACTGGCTTGATATATGTGAACGAACAATTTGACGGATGGAAAGCAGAATGCCTAAGGATACTCCGAAGTAAATTTGACTCGACTAAACGTACATTTGCACCAGACAGCGAAATAATGGAAGCACTACAGAAGAGTTCAGTTGGGCAGGCAGTGGATTTTAGACAAACACAGAAGTTGTGTATGCCATTCTTGAGGTTTAAGAAGGATGAGGCTGCTGCACTCGGGGTTCATGCCTTGGACTTGAAGCTTCCTTTTGGGGAGATGGAGGTCCTTAAAGAGAACTTGGAGTTGATTAAGAGGCAAATAGGTTTGGAAGAGGTGGAGGTTTTGTGTGCATCCGACACCGATGCTCTTACAAAAGCCGGTGCTTTGGCTTCACTGTTGAAGCAGAATCCTCCATCCCCAGGGAATCCTACTGCTATTTTCTTGACAAGTTGA

Protein sequence

MASESGKSFARRDSLREIEAKVRVLWEENDVFRAEACETPPKVGEKFFGNFPFPYMNGFLHLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPREIEEQENLKAEAEDANESNPTLPDKFKGKKSKAASKSSGQMYQWEIMRSFGLSDSEISKFQNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKDVRYTIYSPLDGQPCADHDRATGEGVQPQDYTLIKMEVVPPFPPKMGVLVGRKVYLAAATLRPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSKVPEKPTCLIQLTGNDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRAKYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCLDLKIKSQNEKDKLAEAKRLTYLKGFTDGTLIVGEFAGRKVQEAKPLIRSKLIETGQAIPYSEPEKRVISRSGDECIVALTDQWYITYGESEWKKLAEECLANMQLFSDETRHGFEHTLSWLNQWACSRSFGLGTRIPWDEQFLVESLSDSTIYMAYYTIAHLLHNGDLYGSGASAIKPEQMTDEVWDFVFCGAAEPKSTGISKSILNKMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPRHHWPRGFRCNGHIMLNSEKMSKSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDILQADSASFLRTGPPSTYADRVFENEINIAVKMTEQNYKDYMFREALKTGFYDLQAARDEYRFSCGAAGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPLADSPDLTLKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTTVIEDKKLTGLIYVNEQFDGWKAECLRILRSKFDSTKRTFAPDSEIMEALQKSSVGQAVDFRQTQKLCMPFLRFKKDEAAALGVHALDLKLPFGEMEVLKENLELIKRQIGLEEVEVLCASDTDALTKAGALASLLKQNPPSPGNPTAIFLTS
Homology
BLAST of Spg027529 vs. NCBI nr
Match: XP_022948270.1 (leucine--tRNA ligase, cytoplasmic-like [Cucurbita moschata])

HSP 1 Score: 2173.7 bits (5631), Expect = 0.0e+00
Identity = 1058/1090 (97.06%), Postives = 1075/1090 (98.62%), Query Frame = 0

Query: 1    MASESGKSFARRDSLREIEAKVRVLWEENDVFRAEACETPPKVGEKFFGNFPFPYMNGFL 60
            MASE GKSFARRDSLREIE+KVRVLWEENDVFRAEACETPPK GEKFFGNFPFPYMNGFL
Sbjct: 1    MASECGKSFARRDSLREIESKVRVLWEENDVFRAEACETPPKEGEKFFGNFPFPYMNGFL 60

Query: 61   HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR 120
            HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR
Sbjct: 61   HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR 120

Query: 121  EIEEQENLKAEAEDANESNPTLPDKFKGKKSKAASKSSGQMYQWEIMRSFGLSDSEISKF 180
            ++EEQENLKAE+EDANESNPTLPDKFKGKKSKA SKSSGQMYQWEIMRSFGLSDSEISKF
Sbjct: 121  QMEEQENLKAESEDANESNPTLPDKFKGKKSKAVSKSSGQMYQWEIMRSFGLSDSEISKF 180

Query: 181  QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD 240
            QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD
Sbjct: 181  QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD 240

Query: 241  VRYTIYSPLDGQPCADHDRATGEGVQPQDYTLIKMEVVPPFPPKMGVLVGRKVYLAAATL 300
            VRYTIYSPLDGQPCADHDRA+GEGVQPQDYTLIKMEVVPPFPPKMGVL GRKVYLAAATL
Sbjct: 241  VRYTIYSPLDGQPCADHDRASGEGVQPQDYTLIKMEVVPPFPPKMGVLEGRKVYLAAATL 300

Query: 301  RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSKVPEKPTCLIQLTG 360
            RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFS+VPEKPTCLIQLTG
Sbjct: 301  RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSRVPEKPTCLIQLTG 360

Query: 361  NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 420
            NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA
Sbjct: 361  NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 420

Query: 421  KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCLDLKIKSQNEKDKLAEAKRLTYLKGFTD 480
            KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVC+DLKIKSQNEKDKLAEAKRLTYLKGFTD
Sbjct: 421  KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTD 480

Query: 481  GTLIVGEFAGRKVQEAKPLIRSKLIETGQAIPYSEPEKRVISRSGDECIVALTDQWYITY 540
            GTLIVGEF+GRKVQEAKP+IRSKLIETGQAIPYSEPEKRV SRSGDECIVALTDQWYITY
Sbjct: 481  GTLIVGEFSGRKVQEAKPMIRSKLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITY 540

Query: 541  GESEWKKLAEECLANMQLFSDETRHGFEHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 600
            GESEWKKLAE+CLANM LFSDETRHGF+HTLSWLNQWACSRSFGLGTRIPWDEQFLVESL
Sbjct: 541  GESEWKKLAEDCLANMNLFSDETRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 600

Query: 601  SDSTIYMAYYTIAHLLHNGDLYGSGASAIKPEQMTDEVWDFVFCGAAEPKSTGISKSILN 660
            SDSTIYMAYYTIAHLLHNGDLYGSGASAIKPEQMTDEVWDFVFCGAAEPKSTGIS+SILN
Sbjct: 601  SDSTIYMAYYTIAHLLHNGDLYGSGASAIKPEQMTDEVWDFVFCGAAEPKSTGISRSILN 660

Query: 661  KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPRHHWPRGFRCNGHIMLNSEKMS 720
            KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMP HHWPRGFRCNGHIMLNSEKMS
Sbjct: 661  KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPEHHWPRGFRCNGHIMLNSEKMS 720

Query: 721  KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 780
            KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL
Sbjct: 721  KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 780

Query: 781  QADSASFLRTGPPSTYADRVFENEINIAVKMTEQNYKDYMFREALKTGFYDLQAARDEYR 840
            QADS+SFLRTGPPSTYADRVFENEINIAVK TEQNYKDYMFREALK+GFYDLQAARDEYR
Sbjct: 781  QADSSSFLRTGPPSTYADRVFENEINIAVKTTEQNYKDYMFREALKSGFYDLQAARDEYR 840

Query: 841  FSCGAAGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPLADSPDLT 900
            FSCGA GMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWP A SPDLT
Sbjct: 841  FSCGAGGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPSAGSPDLT 900

Query: 901  LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTTVIEDKKLTGLIYVNEQFDGWKA 960
            LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVT+VIEDKKLTGLIYVNEQFDGWKA
Sbjct: 901  LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTSVIEDKKLTGLIYVNEQFDGWKA 960

Query: 961  ECLRILRSKFDSTKRTFAPDSEIMEALQKSSVGQAVDFRQTQKLCMPFLRFKKDEAAALG 1020
            ECLRILR KFDSTKRTFAPDSEIMEALQKSSVGQA DFRQTQKLCMPFLRFKKDE AALG
Sbjct: 961  ECLRILRCKFDSTKRTFAPDSEIMEALQKSSVGQASDFRQTQKLCMPFLRFKKDEVAALG 1020

Query: 1021 VHALDLKLPFGEMEVLKENLELIKRQIGLEEVEVLCASDTDALTKAGALASLLKQNPPSP 1080
            VHALDLKLPFGE+EVLKENLELIKRQIGLEEVEVLCASDTDA+ KAGALASLLKQNPPSP
Sbjct: 1021 VHALDLKLPFGEIEVLKENLELIKRQIGLEEVEVLCASDTDAIAKAGALASLLKQNPPSP 1080

Query: 1081 GNPTAIFLTS 1091
            GNPTAIFLTS
Sbjct: 1081 GNPTAIFLTS 1090

BLAST of Spg027529 vs. NCBI nr
Match: KAG7036878.1 (Leucine--tRNA ligase, cytoplasmic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 2173.7 bits (5631), Expect = 0.0e+00
Identity = 1058/1090 (97.06%), Postives = 1075/1090 (98.62%), Query Frame = 0

Query: 1    MASESGKSFARRDSLREIEAKVRVLWEENDVFRAEACETPPKVGEKFFGNFPFPYMNGFL 60
            MASE GKSFARRDSLREIE+KVRVLWEENDVFRAEACETPPK GEKFFGNFPFPYMNGFL
Sbjct: 19   MASECGKSFARRDSLREIESKVRVLWEENDVFRAEACETPPKEGEKFFGNFPFPYMNGFL 78

Query: 61   HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR 120
            HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR
Sbjct: 79   HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR 138

Query: 121  EIEEQENLKAEAEDANESNPTLPDKFKGKKSKAASKSSGQMYQWEIMRSFGLSDSEISKF 180
            ++EEQENLKAE+EDANESNPTLPDKFKGKKSKA SKSSGQMYQWEIMRSFGLSDSEISKF
Sbjct: 139  QMEEQENLKAESEDANESNPTLPDKFKGKKSKAVSKSSGQMYQWEIMRSFGLSDSEISKF 198

Query: 181  QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD 240
            QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD
Sbjct: 199  QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD 258

Query: 241  VRYTIYSPLDGQPCADHDRATGEGVQPQDYTLIKMEVVPPFPPKMGVLVGRKVYLAAATL 300
            VRYTIYSPLDGQPCADHDRA+GEGVQPQDYTLIKMEVVPPFPPKMGVL GRKVYLAAATL
Sbjct: 259  VRYTIYSPLDGQPCADHDRASGEGVQPQDYTLIKMEVVPPFPPKMGVLEGRKVYLAAATL 318

Query: 301  RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSKVPEKPTCLIQLTG 360
            RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFS+VPEKPTCLIQLTG
Sbjct: 319  RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSRVPEKPTCLIQLTG 378

Query: 361  NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 420
            NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA
Sbjct: 379  NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 438

Query: 421  KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCLDLKIKSQNEKDKLAEAKRLTYLKGFTD 480
            KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVC+DLKIKSQNEKDKLAEAKRLTYLKGFTD
Sbjct: 439  KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTD 498

Query: 481  GTLIVGEFAGRKVQEAKPLIRSKLIETGQAIPYSEPEKRVISRSGDECIVALTDQWYITY 540
            GTLIVGEF+GRKVQEAKP+IRSKLIETGQAIPYSEPEKRV SRSGDECIVALTDQWYITY
Sbjct: 499  GTLIVGEFSGRKVQEAKPMIRSKLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITY 558

Query: 541  GESEWKKLAEECLANMQLFSDETRHGFEHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 600
            GESEWKKLAE+CLANM LFSDETRHGF+HTLSWLNQWACSRSFGLGTRIPWDEQFLVESL
Sbjct: 559  GESEWKKLAEDCLANMNLFSDETRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 618

Query: 601  SDSTIYMAYYTIAHLLHNGDLYGSGASAIKPEQMTDEVWDFVFCGAAEPKSTGISKSILN 660
            SDSTIYMAYYTIAHLLHNGDLYGSGASAIKPEQMTDEVWDFVFCGAAEPKSTGIS+SILN
Sbjct: 619  SDSTIYMAYYTIAHLLHNGDLYGSGASAIKPEQMTDEVWDFVFCGAAEPKSTGISRSILN 678

Query: 661  KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPRHHWPRGFRCNGHIMLNSEKMS 720
            KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMP HHWPRGFRCNGHIMLNSEKMS
Sbjct: 679  KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPEHHWPRGFRCNGHIMLNSEKMS 738

Query: 721  KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 780
            KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL
Sbjct: 739  KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 798

Query: 781  QADSASFLRTGPPSTYADRVFENEINIAVKMTEQNYKDYMFREALKTGFYDLQAARDEYR 840
            QADS+SFLRTGPPSTYADRVFENEINIAVK TEQNYKDYMFREALK+GFYDLQAARDEYR
Sbjct: 799  QADSSSFLRTGPPSTYADRVFENEINIAVKTTEQNYKDYMFREALKSGFYDLQAARDEYR 858

Query: 841  FSCGAAGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPLADSPDLT 900
            FSCGA GMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWP A SPDLT
Sbjct: 859  FSCGAGGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPSAGSPDLT 918

Query: 901  LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTTVIEDKKLTGLIYVNEQFDGWKA 960
            LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVT+VIEDKKLTGLIYVNEQFDGWKA
Sbjct: 919  LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTSVIEDKKLTGLIYVNEQFDGWKA 978

Query: 961  ECLRILRSKFDSTKRTFAPDSEIMEALQKSSVGQAVDFRQTQKLCMPFLRFKKDEAAALG 1020
            ECLRILR KFDSTKRTFAPDSEIMEALQKSSVGQA DFRQTQKLCMPFLRFKKDE AALG
Sbjct: 979  ECLRILRCKFDSTKRTFAPDSEIMEALQKSSVGQASDFRQTQKLCMPFLRFKKDEVAALG 1038

Query: 1021 VHALDLKLPFGEMEVLKENLELIKRQIGLEEVEVLCASDTDALTKAGALASLLKQNPPSP 1080
            VHALDLKLPFGE+EVLKENLELIKRQIGLEEVEVLCASDTDA+ KAGALASLLKQNPPSP
Sbjct: 1039 VHALDLKLPFGEIEVLKENLELIKRQIGLEEVEVLCASDTDAIAKAGALASLLKQNPPSP 1098

Query: 1081 GNPTAIFLTS 1091
            GNPTAIFLTS
Sbjct: 1099 GNPTAIFLTS 1108

BLAST of Spg027529 vs. NCBI nr
Match: KAG6607189.1 (Leucine--tRNA ligase, cytoplasmic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2173.7 bits (5631), Expect = 0.0e+00
Identity = 1058/1090 (97.06%), Postives = 1075/1090 (98.62%), Query Frame = 0

Query: 1    MASESGKSFARRDSLREIEAKVRVLWEENDVFRAEACETPPKVGEKFFGNFPFPYMNGFL 60
            MASE GKSFARRDSLREIE+KVRVLWEENDVFRAEACETPPK GEKFFGNFPFPYMNGFL
Sbjct: 72   MASECGKSFARRDSLREIESKVRVLWEENDVFRAEACETPPKEGEKFFGNFPFPYMNGFL 131

Query: 61   HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR 120
            HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR
Sbjct: 132  HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR 191

Query: 121  EIEEQENLKAEAEDANESNPTLPDKFKGKKSKAASKSSGQMYQWEIMRSFGLSDSEISKF 180
            ++EEQENLKAE+EDANESNPTLPDKFKGKKSKA SKSSGQMYQWEIMRSFGLSDSEISKF
Sbjct: 192  QMEEQENLKAESEDANESNPTLPDKFKGKKSKAVSKSSGQMYQWEIMRSFGLSDSEISKF 251

Query: 181  QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD 240
            QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD
Sbjct: 252  QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD 311

Query: 241  VRYTIYSPLDGQPCADHDRATGEGVQPQDYTLIKMEVVPPFPPKMGVLVGRKVYLAAATL 300
            VRYTIYSPLDGQPCADHDRA+GEGVQPQDYTLIKMEVVPPFPPKMGVL GRKVYLAAATL
Sbjct: 312  VRYTIYSPLDGQPCADHDRASGEGVQPQDYTLIKMEVVPPFPPKMGVLEGRKVYLAAATL 371

Query: 301  RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSKVPEKPTCLIQLTG 360
            RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFS+VPEKPTCLIQLTG
Sbjct: 372  RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSRVPEKPTCLIQLTG 431

Query: 361  NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 420
            NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA
Sbjct: 432  NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 491

Query: 421  KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCLDLKIKSQNEKDKLAEAKRLTYLKGFTD 480
            KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVC+DLKIKSQNEKDKLAEAKRLTYLKGFTD
Sbjct: 492  KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTD 551

Query: 481  GTLIVGEFAGRKVQEAKPLIRSKLIETGQAIPYSEPEKRVISRSGDECIVALTDQWYITY 540
            GTLIVGEF+GRKVQEAKP+IRSKLIETGQAIPYSEPEKRV SRSGDECIVALTDQWYITY
Sbjct: 552  GTLIVGEFSGRKVQEAKPMIRSKLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITY 611

Query: 541  GESEWKKLAEECLANMQLFSDETRHGFEHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 600
            GESEWKKLAE+CLANM LFSDETRHGF+HTLSWLNQWACSRSFGLGTRIPWDEQFLVESL
Sbjct: 612  GESEWKKLAEDCLANMNLFSDETRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 671

Query: 601  SDSTIYMAYYTIAHLLHNGDLYGSGASAIKPEQMTDEVWDFVFCGAAEPKSTGISKSILN 660
            SDSTIYMAYYTIAHLLHNGDLYGSGASAIKPEQMTDEVWDFVFCGAAEPKSTGIS+SILN
Sbjct: 672  SDSTIYMAYYTIAHLLHNGDLYGSGASAIKPEQMTDEVWDFVFCGAAEPKSTGISRSILN 731

Query: 661  KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPRHHWPRGFRCNGHIMLNSEKMS 720
            KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMP HHWPRGFRCNGHIMLNSEKMS
Sbjct: 732  KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPEHHWPRGFRCNGHIMLNSEKMS 791

Query: 721  KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 780
            KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL
Sbjct: 792  KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 851

Query: 781  QADSASFLRTGPPSTYADRVFENEINIAVKMTEQNYKDYMFREALKTGFYDLQAARDEYR 840
            QADS+SFLRTGPPSTYADRVFENEINIAVK TEQNYKDYMFREALK+GFYDLQAARDEYR
Sbjct: 852  QADSSSFLRTGPPSTYADRVFENEINIAVKTTEQNYKDYMFREALKSGFYDLQAARDEYR 911

Query: 841  FSCGAAGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPLADSPDLT 900
            FSCGA GMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWP A SPDLT
Sbjct: 912  FSCGAGGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPSAGSPDLT 971

Query: 901  LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTTVIEDKKLTGLIYVNEQFDGWKA 960
            LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVT+VIEDKKLTGLIYVNEQFDGWKA
Sbjct: 972  LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTSVIEDKKLTGLIYVNEQFDGWKA 1031

Query: 961  ECLRILRSKFDSTKRTFAPDSEIMEALQKSSVGQAVDFRQTQKLCMPFLRFKKDEAAALG 1020
            ECLRILR KFDSTKRTFAPDSEIMEALQKSSVGQA DFRQTQKLCMPFLRFKKDE AALG
Sbjct: 1032 ECLRILRCKFDSTKRTFAPDSEIMEALQKSSVGQASDFRQTQKLCMPFLRFKKDEVAALG 1091

Query: 1021 VHALDLKLPFGEMEVLKENLELIKRQIGLEEVEVLCASDTDALTKAGALASLLKQNPPSP 1080
            VHALDLKLPFGE+EVLKENLELIKRQIGLEEVEVLCASDTDA+ KAGALASLLKQNPPSP
Sbjct: 1092 VHALDLKLPFGEIEVLKENLELIKRQIGLEEVEVLCASDTDAIAKAGALASLLKQNPPSP 1151

Query: 1081 GNPTAIFLTS 1091
            GNPTAIFLTS
Sbjct: 1152 GNPTAIFLTS 1161

BLAST of Spg027529 vs. NCBI nr
Match: XP_023523654.1 (leucine--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2167.1 bits (5614), Expect = 0.0e+00
Identity = 1054/1090 (96.70%), Postives = 1073/1090 (98.44%), Query Frame = 0

Query: 1    MASESGKSFARRDSLREIEAKVRVLWEENDVFRAEACETPPKVGEKFFGNFPFPYMNGFL 60
            MASE GKSFARRDSLREIE+KVRVLWEENDVFRAEACETPPK GEKFFGNFPFPYMNGFL
Sbjct: 1    MASECGKSFARRDSLREIESKVRVLWEENDVFRAEACETPPKEGEKFFGNFPFPYMNGFL 60

Query: 61   HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR 120
            HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR
Sbjct: 61   HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR 120

Query: 121  EIEEQENLKAEAEDANESNPTLPDKFKGKKSKAASKSSGQMYQWEIMRSFGLSDSEISKF 180
            ++EEQENLKAE+EDANESNPTLPDKFKGKKSKA SKSSGQMYQWEIMRSFGLSDSEISKF
Sbjct: 121  QMEEQENLKAESEDANESNPTLPDKFKGKKSKAVSKSSGQMYQWEIMRSFGLSDSEISKF 180

Query: 181  QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD 240
            Q PYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD
Sbjct: 181  QTPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD 240

Query: 241  VRYTIYSPLDGQPCADHDRATGEGVQPQDYTLIKMEVVPPFPPKMGVLVGRKVYLAAATL 300
            VRYTIYSPLDGQPCADHDRA+GEGVQPQDYTLIKMEVVPPFPPKMGVL GRKVYLAAATL
Sbjct: 241  VRYTIYSPLDGQPCADHDRASGEGVQPQDYTLIKMEVVPPFPPKMGVLEGRKVYLAAATL 300

Query: 301  RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSKVPEKPTCLIQLTG 360
            RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITER+ALNLAYQKFS+VPEKPTCLIQLTG
Sbjct: 301  RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERSALNLAYQKFSRVPEKPTCLIQLTG 360

Query: 361  NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 420
            NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA
Sbjct: 361  NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 420

Query: 421  KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCLDLKIKSQNEKDKLAEAKRLTYLKGFTD 480
            KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVC+DLKIKSQNEKDKLAEAKRLTYLKGFTD
Sbjct: 421  KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTD 480

Query: 481  GTLIVGEFAGRKVQEAKPLIRSKLIETGQAIPYSEPEKRVISRSGDECIVALTDQWYITY 540
            GTLIVGEF+GRKVQEAKP+IRSKLIETGQAIPYSEPEKRV SRSGDECIVALTDQWYITY
Sbjct: 481  GTLIVGEFSGRKVQEAKPMIRSKLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITY 540

Query: 541  GESEWKKLAEECLANMQLFSDETRHGFEHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 600
            GESEWKKLAE+CLANM LFSDETRHGF+HTLSWLNQWACSRSFGLGTRIPWDEQFLVESL
Sbjct: 541  GESEWKKLAEDCLANMNLFSDETRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 600

Query: 601  SDSTIYMAYYTIAHLLHNGDLYGSGASAIKPEQMTDEVWDFVFCGAAEPKSTGISKSILN 660
            SDSTIYMAYYTIAHLLHNGDLYGSG SAIKPEQMTDEVWDFVFCGAAEPKSTGIS+SILN
Sbjct: 601  SDSTIYMAYYTIAHLLHNGDLYGSGTSAIKPEQMTDEVWDFVFCGAAEPKSTGISRSILN 660

Query: 661  KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPRHHWPRGFRCNGHIMLNSEKMS 720
            KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMP HHWPRGFRCNGHIMLNSEKMS
Sbjct: 661  KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPEHHWPRGFRCNGHIMLNSEKMS 720

Query: 721  KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 780
            KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL
Sbjct: 721  KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 780

Query: 781  QADSASFLRTGPPSTYADRVFENEINIAVKMTEQNYKDYMFREALKTGFYDLQAARDEYR 840
            QADS+SFLR GPPSTYADRVFENEINIAVK TEQNYKDYMFREALK+GFYDLQAARDEYR
Sbjct: 781  QADSSSFLRKGPPSTYADRVFENEINIAVKTTEQNYKDYMFREALKSGFYDLQAARDEYR 840

Query: 841  FSCGAAGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPLADSPDLT 900
            FSCGA GMNR+LVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWP ADSPDLT
Sbjct: 841  FSCGAGGMNRNLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPSADSPDLT 900

Query: 901  LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTTVIEDKKLTGLIYVNEQFDGWKA 960
            LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVT+VIEDKKLTGLIYVNEQFDGWKA
Sbjct: 901  LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTSVIEDKKLTGLIYVNEQFDGWKA 960

Query: 961  ECLRILRSKFDSTKRTFAPDSEIMEALQKSSVGQAVDFRQTQKLCMPFLRFKKDEAAALG 1020
            ECLRILR KFDSTKRTFAPDSEIMEALQKSSVGQA DFRQTQKLCMPFLRFKKDE AALG
Sbjct: 961  ECLRILRCKFDSTKRTFAPDSEIMEALQKSSVGQASDFRQTQKLCMPFLRFKKDEVAALG 1020

Query: 1021 VHALDLKLPFGEMEVLKENLELIKRQIGLEEVEVLCASDTDALTKAGALASLLKQNPPSP 1080
            VHALDLKLPFGE+EVLKENLELIKRQIGLEEVEVLCASDTDA+ KAGALASLLKQNPPSP
Sbjct: 1021 VHALDLKLPFGEIEVLKENLELIKRQIGLEEVEVLCASDTDAIAKAGALASLLKQNPPSP 1080

Query: 1081 GNPTAIFLTS 1091
            GNPTAIFLTS
Sbjct: 1081 GNPTAIFLTS 1090

BLAST of Spg027529 vs. NCBI nr
Match: XP_022998939.1 (leucine--tRNA ligase, cytoplasmic-like [Cucurbita maxima])

HSP 1 Score: 2149.4 bits (5568), Expect = 0.0e+00
Identity = 1046/1090 (95.96%), Postives = 1070/1090 (98.17%), Query Frame = 0

Query: 1    MASESGKSFARRDSLREIEAKVRVLWEENDVFRAEACETPPKVGEKFFGNFPFPYMNGFL 60
            M SE GKSFARRDSLREIE+KVRVLWEENDVFRAEACETPPK GEKFFGNFPFPYMNGFL
Sbjct: 1    MTSECGKSFARRDSLREIESKVRVLWEENDVFRAEACETPPKEGEKFFGNFPFPYMNGFL 60

Query: 61   HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR 120
            HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREI+QFGDPPVFP 
Sbjct: 61   HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIKQFGDPPVFPS 120

Query: 121  EIEEQENLKAEAEDANESNPTLPDKFKGKKSKAASKSSGQMYQWEIMRSFGLSDSEISKF 180
            +IEE+ENLKAE+EDANESNPTLPDKFKGKKSKA SKSSGQMYQWEIMRSFGLSDSEISKF
Sbjct: 121  QIEEEENLKAESEDANESNPTLPDKFKGKKSKAVSKSSGQMYQWEIMRSFGLSDSEISKF 180

Query: 181  QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD 240
            QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD
Sbjct: 181  QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD 240

Query: 241  VRYTIYSPLDGQPCADHDRATGEGVQPQDYTLIKMEVVPPFPPKMGVLVGRKVYLAAATL 300
            VRYTIYSPLDGQPCADHDRA+GEGVQPQDYTLIKMEVVPPFPPKM VL GRKVYLAAATL
Sbjct: 241  VRYTIYSPLDGQPCADHDRASGEGVQPQDYTLIKMEVVPPFPPKMSVLEGRKVYLAAATL 300

Query: 301  RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSKVPEKPTCLIQLTG 360
            RPETMYGQTNAWVLPEGKYGAFEINDTDV+IITERAALNLAYQKFS+VPEKPTCLIQLTG
Sbjct: 301  RPETMYGQTNAWVLPEGKYGAFEINDTDVYIITERAALNLAYQKFSRVPEKPTCLIQLTG 360

Query: 361  NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 420
            NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA
Sbjct: 361  NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 420

Query: 421  KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCLDLKIKSQNEKDKLAEAKRLTYLKGFTD 480
            KYGVKDEWVLPYDIVPII+IPEFGDRAAEKVC+DLKIKSQNEKDKLAEAKRLTYLKGFTD
Sbjct: 421  KYGVKDEWVLPYDIVPIINIPEFGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTD 480

Query: 481  GTLIVGEFAGRKVQEAKPLIRSKLIETGQAIPYSEPEKRVISRSGDECIVALTDQWYITY 540
            GTLIVGEF+GRKVQEAKP+IRSKLIETGQAIPYSEPEKRVISRSGDECIVALTDQWYITY
Sbjct: 481  GTLIVGEFSGRKVQEAKPMIRSKLIETGQAIPYSEPEKRVISRSGDECIVALTDQWYITY 540

Query: 541  GESEWKKLAEECLANMQLFSDETRHGFEHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 600
            GESEWKKLAE+CLANM LFSDETRHGF+HTLSWLNQWACSRSFGLGTRIPWDEQFLVESL
Sbjct: 541  GESEWKKLAEDCLANMNLFSDETRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 600

Query: 601  SDSTIYMAYYTIAHLLHNGDLYGSGASAIKPEQMTDEVWDFVFCGAAEPKSTGISKSILN 660
            SDSTIYMAYYTIAHLLH+GDLYGSGASAIKPEQMTDEVWDFVF GAAEPKSTGIS+SILN
Sbjct: 601  SDSTIYMAYYTIAHLLHSGDLYGSGASAIKPEQMTDEVWDFVFFGAAEPKSTGISRSILN 660

Query: 661  KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPRHHWPRGFRCNGHIMLNSEKMS 720
            KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMP HHWPRGFRCNGHIMLNSEKMS
Sbjct: 661  KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPEHHWPRGFRCNGHIMLNSEKMS 720

Query: 721  KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 780
            KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL
Sbjct: 721  KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 780

Query: 781  QADSASFLRTGPPSTYADRVFENEINIAVKMTEQNYKDYMFREALKTGFYDLQAARDEYR 840
            QADS+SFLRTGPPSTYADRVFENE+NIAVK TEQNYK+YMFREALK+GFYDLQAARDEYR
Sbjct: 781  QADSSSFLRTGPPSTYADRVFENEMNIAVKTTEQNYKNYMFREALKSGFYDLQAARDEYR 840

Query: 841  FSCGAAGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPLADSPDLT 900
            FSCGA GMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWP A  PDLT
Sbjct: 841  FSCGAGGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPSAGYPDLT 900

Query: 901  LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTTVIEDKKLTGLIYVNEQFDGWKA 960
            LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVT VIEDKKLTGLIYVNEQFDGWKA
Sbjct: 901  LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTYVIEDKKLTGLIYVNEQFDGWKA 960

Query: 961  ECLRILRSKFDSTKRTFAPDSEIMEALQKSSVGQAVDFRQTQKLCMPFLRFKKDEAAALG 1020
            ECLRIL+ KFDSTKRTFAPDSEIMEALQKSSVGQA DFRQTQKLCMPFLRFKKDE AALG
Sbjct: 961  ECLRILQCKFDSTKRTFAPDSEIMEALQKSSVGQASDFRQTQKLCMPFLRFKKDEVAALG 1020

Query: 1021 VHALDLKLPFGEMEVLKENLELIKRQIGLEEVEVLCASDTDALTKAGALASLLKQNPPSP 1080
            VHALDLKLPFGE+EVLKENLELIKRQIGLEEVEVLCASDTDA+ K+GALASLLKQNPPSP
Sbjct: 1021 VHALDLKLPFGEIEVLKENLELIKRQIGLEEVEVLCASDTDAIAKSGALASLLKQNPPSP 1080

Query: 1081 GNPTAIFLTS 1091
            GNPTAIFLTS
Sbjct: 1081 GNPTAIFLTS 1090

BLAST of Spg027529 vs. ExPASy Swiss-Prot
Match: F4I116 (Leucine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At1g09620 PE=1 SV=1)

HSP 1 Score: 1691.4 bits (4379), Expect = 0.0e+00
Identity = 804/1090 (73.76%), Postives = 950/1090 (87.16%), Query Frame = 0

Query: 1    MASESGKSFARRDSLREIEAKVRVLWEENDVFRAEACETPPKVGEKFFGNFPFPYMNGFL 60
            MASES KS+ARRD L EIEA VR  WE+ DVFRAE+CE  PK GEKFF  FPFPYMNG+L
Sbjct: 1    MASES-KSYARRDRLLEIEATVRKWWEDEDVFRAESCENLPKPGEKFFSTFPFPYMNGYL 60

Query: 61   HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR 120
            H+GHAFSLSK++FA+AYHRLRGANVLLPFGFHCTGMPIKASADKL REI+QFG+PPVF  
Sbjct: 61   HIGHAFSLSKVDFASAYHRLRGANVLLPFGFHCTGMPIKASADKLRREIEQFGNPPVFTA 120

Query: 121  EIEEQENLKAEAEDANESNPTLPDKFKGKKSKAASKSSGQMYQWEIMRSFGLSDSEISKF 180
            E +  +  + + E ++     +P +FKGKKSK A+K+ GQ+YQWEIMRSFGL+DSEI+ F
Sbjct: 121  E-DTTKVPEVQEESSDTIALPIPGQFKGKKSKVAAKAGGQVYQWEIMRSFGLTDSEIANF 180

Query: 181  QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD 240
            + P  WL +FPPLA+EDL+A+GLGCDWRRSF+TTD+NPFFD+FV+WQMRKLKSMGKIVKD
Sbjct: 181  REPSEWLYYFPPLAVEDLRAYGLGCDWRRSFVTTDVNPFFDAFVRWQMRKLKSMGKIVKD 240

Query: 241  VRYTIYSPLDGQPCADHDRATGEGVQPQDYTLIKMEVVPPFPPKMGVLVGRKVYLAAATL 300
             RYTI+SPLDGQPCADHDRATGEGVQPQ+YTLIKMEVV PFP K+G L G++V+LAAATL
Sbjct: 241  RRYTIFSPLDGQPCADHDRATGEGVQPQEYTLIKMEVVKPFPLKLGPLEGKRVFLAAATL 300

Query: 301  RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSKVPEKPTCLIQLTG 360
            RPETMYGQTNAWVLP+GKYGA+EI++T+VFI+TERAALNLAYQ FSK P++P+CL++LTG
Sbjct: 301  RPETMYGQTNAWVLPDGKYGAYEISETEVFILTERAALNLAYQNFSKNPQEPSCLVELTG 360

Query: 361  NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 420
             DLIGLPL+SPL+ NEIIYALPMLTILT+KGTGIVTSVPSDAPDDYMA+ DL  KPAL+ 
Sbjct: 361  YDLIGLPLRSPLSVNEIIYALPMLTILTNKGTGIVTSVPSDAPDDYMALQDLIKKPALQD 420

Query: 421  KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCLDLKIKSQNEKDKLAEAKRLTYLKGFTD 480
            KYGVK EW LP +I+PII+IPEFGD+AAEKVCLDLKIKSQN+K+KLAEAKRLTYLKGFT+
Sbjct: 421  KYGVKTEW-LPTEIIPIINIPEFGDKAAEKVCLDLKIKSQNDKEKLAEAKRLTYLKGFTE 480

Query: 481  GTLIVGEFAGRKVQEAKPLIRSKLIETGQAIPYSEPEKRVISRSGDECIVALTDQWYITY 540
            GT+++GEF GRKVQE KP+I++KLIETG+AI YSEPEK V+SRSGDEC+VALTDQWYITY
Sbjct: 481  GTMLIGEFFGRKVQEIKPIIKTKLIETGEAIIYSEPEKPVMSRSGDECVVALTDQWYITY 540

Query: 541  GESEWKKLAEECLANMQLFSDETRHGFEHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 600
            GESEW+K+AEECL+ M L+SDETRHGFEHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL
Sbjct: 541  GESEWRKIAEECLSKMNLYSDETRHGFEHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 600

Query: 601  SDSTIYMAYYTIAHLLHNGDLYGSGASAIKPEQMTDEVWDFVFCGAAEPKSTGISKSILN 660
            SDS++YMAYYT+AH+ H+GD+Y    S I+P+QM DEVW+++FC    PKS+ I  ++L+
Sbjct: 601  SDSSLYMAYYTVAHIFHDGDMYKGSKSLIRPQQMNDEVWEYLFCDGPYPKSSDIPSAVLS 660

Query: 661  KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPRHHWPRGFRCNGHIMLNSEKMS 720
            +MKQEF+YWYP DLRVSGKDLIQNHLTF IYNHTA+M   +WPRG RCNGHIMLNSEKMS
Sbjct: 661  EMKQEFDYWYPLDLRVSGKDLIQNHLTFFIYNHTALMANRNWPRGIRCNGHIMLNSEKMS 720

Query: 721  KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 780
            KSTGNFRTLRQ+IEEFSA  TRF LADAGDGVDDANF FETANAAILRLTKE+ WME++L
Sbjct: 721  KSTGNFRTLRQSIEEFSATGTRFCLADAGDGVDDANFAFETANAAILRLTKELTWMEEVL 780

Query: 781  QADSASFLRTGPPSTYADRVFENEINIAVKMTEQNYKDYMFREALKTGFYDLQAARDEYR 840
              +S+  LRTGPPSTYAD+VFEN++NIA+++TE+ YKD +FREALK GFYDLQAARDEYR
Sbjct: 781  DVESS--LRTGPPSTYADKVFENDMNIALRLTERAYKDCLFREALKNGFYDLQAARDEYR 840

Query: 841  FSCGAAGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPLADSPDLT 900
             SCG  GM+ DL+ +FMDVQTRLI PICPH+A+YVWR++L KEG V+ AGWP ++ PDL 
Sbjct: 841  LSCGTGGMHHDLLLKFMDVQTRLIVPICPHFADYVWRKVLNKEGCVLTAGWPPSNEPDLV 900

Query: 901  LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTTVIEDKKLTGLIYVNEQFDGWKA 960
            LKSANKYLQDSIVLMRKLLQKQL GSKKG KKGA VT V E  KL GL+YVNEQFDGW+A
Sbjct: 901  LKSANKYLQDSIVLMRKLLQKQLSGSKKGAKKGAQVTAVPEG-KLKGLVYVNEQFDGWRA 960

Query: 961  ECLRILRSKFDSTKRTFAPDSEIMEALQKSSVGQAVDFRQTQKLCMPFLRFKKDEAAALG 1020
             CLRIL+S+FD    +F PD+E++  L  + + +  + +  QK+CMPFL+FKKDEA ++G
Sbjct: 961  HCLRILQSRFDQQTCSFPPDTEMLAELSATLLQEGKNLKAIQKVCMPFLKFKKDEAISIG 1020

Query: 1021 VHALDLKLPFGEMEVLKENLELIKRQIGLEEVEVLCASDTDALTKAGALASLLKQNPPSP 1080
              AL+L+LPFGE+EVL+ N +LI+RQ+GLEEVE+  ASD D ++ AG  ASLL QNPPSP
Sbjct: 1021 TQALNLRLPFGEIEVLQSNKDLIRRQLGLEEVEIYSASDPDDVSIAGPHASLLTQNPPSP 1080

Query: 1081 GNPTAIFLTS 1091
            G+PTAIF+TS
Sbjct: 1081 GSPTAIFVTS 1084

BLAST of Spg027529 vs. ExPASy Swiss-Prot
Match: Q5R614 (Leucine--tRNA ligase, cytoplasmic OS=Pongo abelii OX=9601 GN=LARS1 PE=2 SV=1)

HSP 1 Score: 1080.1 bits (2792), Expect = 0.0e+00
Identity = 533/1060 (50.28%), Postives = 721/1060 (68.02%), Query Frame = 0

Query: 7    KSFARRDSLREIEAKVRVLWEENDVFRAEACETPPKVGE-KFFGNFPFPYMNGFLHLGHA 66
            K  A+ D L++IE +++  W+   VF   A     +  + K+F  FP+PYMNG LHLGH 
Sbjct: 5    KGTAKVDFLKKIEKEIQQKWDTERVFEVNASSLEKQTSKGKYFVTFPYPYMNGRLHLGHT 64

Query: 67   FSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPREIEEQ 126
            FSLSK EFA  Y RL+G   L PFG HCTGMPIKA ADKL REI+ +G PP FP E +E+
Sbjct: 65   FSLSKCEFAVGYQRLKGKCCLFPFGLHCTGMPIKACADKLKREIELYGCPPDFPDEEDEE 124

Query: 127  ENLKAEAEDANESNPTLPDKFKGKKSKAASKSSGQMYQWEIMRSFGLSDSEISKFQNPYN 186
            E    + ED       + DK KGKKSKAA+K+    YQW IM+S GLSD EI KF    +
Sbjct: 125  EETNVKTEDTR-----IKDKAKGKKSKAAAKAGSSKYQWGIMKSLGLSDEEIVKFSEAEH 184

Query: 187  WLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKDVRYTI 246
            WL +FPPLA++DLK  GL  DWRRSFITTD+NP++DSFV+WQ   L+   KI    RYTI
Sbjct: 185  WLDYFPPLAIQDLKRLGLKVDWRRSFITTDVNPYYDSFVRWQFLTLRERNKIKFGKRYTI 244

Query: 247  YSPLDGQPCADHDRATGEGVQPQDYTLIKMEVVPPFPPKMGVLVGRKVYLAAATLRPETM 306
            YSP DGQPC DHDR TGEGV PQ+YTL+K++V+ P+P K+  L G+ ++L AATLRPET+
Sbjct: 245  YSPKDGQPCMDHDRQTGEGVGPQEYTLLKLKVLEPYPSKLSGLKGKNIFLVAATLRPETL 304

Query: 307  YGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSKVPEKPTCLIQLTGNDLIG 366
            +GQTN WV P+ KY  FE  + D+FI T++AA N++YQ F+K       + +L G +++G
Sbjct: 305  FGQTNCWVRPDMKYIGFETVNGDIFICTQKAARNMSYQGFTKDNGVVPVVKELMGEEILG 364

Query: 367  LPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRAKYGVK 426
              L +PL   ++IY LPMLTI  DKGTG+VTSVPSD+PDD  A+ DLK K ALRAKYG++
Sbjct: 365  ASLSAPLTSYKVIYVLPMLTIKEDKGTGVVTSVPSDSPDDIAALRDLKKKQALRAKYGIR 424

Query: 427  DEWVLPYDIVPIIDIPEFGDRAAEKVCLDLKIKSQNEKDKLAEAKRLTYLKGFTDGTLIV 486
            D+ VLP++ VP+I+IP FG+ +A  +C +LKI+SQN+++KLAEAK   YLKGF +G ++V
Sbjct: 425  DDMVLPFEPVPVIEIPGFGNLSAVTICDELKIQSQNDREKLAEAKEKIYLKGFYEGIMLV 484

Query: 487  GEFAGRKVQEAKPLIRSKLIETGQAIPYSEPEKRVISRSGDECIVALTDQWYITYGESEW 546
              F G+KVQ+ K  I+ K+I+ G A+ Y EPEK+V+SRS DEC+VAL DQWY+ YGE  W
Sbjct: 485  DGFKGQKVQDVKKTIQKKMIDAGDALIYMEPEKQVMSRSSDECVVALCDQWYLDYGEENW 544

Query: 547  KKLAEECLANMQLFSDETRHGFEHTLSWLNQWACSRSFGLGTRIPWDEQFLVESLSDSTI 606
            KK   +CL N++ F +ETR  FE TL WL + ACSR++GLGT +PWDEQ+L+ESLSDSTI
Sbjct: 545  KKQTSQCLKNLETFCEETRRNFEATLGWLQEHACSRTYGLGTHLPWDEQWLIESLSDSTI 604

Query: 607  YMAYYTIAHLLHNGDLYGSGAS--AIKPEQMTDEVWDFVFCGAAEPKSTGISKSILNKMK 666
            YMA+YT+AHLL  G+L+G   S   I+P+QMT EVWD+VF   A    T I+K  L+++K
Sbjct: 605  YMAFYTVAHLLQGGNLHGQAESPLGIRPQQMTKEVWDYVFFKEAPFPKTQIAKEKLDQLK 664

Query: 667  QEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPRH--HWPRGFRCNGHIMLNSEKMSK 726
            QEFE+WYP DLRVSGKDL+ NHL++ +YNH A+ P     WP   R NGH++LNSEKMSK
Sbjct: 665  QEFEFWYPVDLRVSGKDLVPNHLSYYLYNHVAMWPEQSDKWPTAVRANGHLLLNSEKMSK 724

Query: 727  STGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDILQ 786
            STGNF TL QAI++FSAD  R +LADAGD V+DANFV   A+A ILRL   + W+++++ 
Sbjct: 725  STGNFLTLTQAIDKFSADGMRLALADAGDTVEDANFVEAMADAGILRLYTWVEWVKEMVA 784

Query: 787  ADSASFLRTGPPSTYADRVFENEINIAVKMTEQNYKDYMFREALKTGFYDLQAARDEYRF 846
              +   LR+GP +T+ DRVF +E+N  +  T+QNY+  MF+EALKTGF++ QAA+D+YR 
Sbjct: 785  --NWDSLRSGPANTFNDRVFASELNAGIIKTDQNYEKMMFKEALKTGFFEFQAAKDKYR- 844

Query: 847  SCGAAGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPLADSPDLTL 906
                 GM+R+LVFRF++VQT L+ P CPH  E++W  +L K   ++NA WP+A   D  L
Sbjct: 845  ELAVEGMHRELVFRFIEVQTLLLAPFCPHLCEHIW-TLLGKPDSIMNASWPVAGPVDEVL 904

Query: 907  KSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTTVIEDKKLTGLIYVNEQFDGWKAE 966
              +++YL +    +R  L+  ++ +K       P+      K     IYV + +  W+  
Sbjct: 905  IHSSQYLMEVTHDLRLRLKNYMMPAKGKKTDKQPL-----QKPSHCTIYVAKNYPPWQHT 964

Query: 967  CLRILRSKFDSTKRTFAPDSEIMEALQKSSVGQAVDFRQTQKLCMPFLRFKKDEAAALGV 1026
             L +LR  F++  R   PD++++     S +G   + ++  K  MPF+   K+    +G 
Sbjct: 965  TLSVLRKHFEANNRKL-PDNKVI----ASELGSMPELKKYMKKVMPFVAMIKENLEKMGP 1024

Query: 1027 HALDLKLPFGEMEVLKENLELIKRQIGLEEVEVLCASDTD 1062
              LDL+L F E  VL EN+  +   + LE +EV  AS+ +
Sbjct: 1025 RILDLQLEFDEKAVLMENIVYLTNSLELEHIEVKFASEAE 1045

BLAST of Spg027529 vs. ExPASy Swiss-Prot
Match: Q9P2J5 (Leucine--tRNA ligase, cytoplasmic OS=Homo sapiens OX=9606 GN=LARS1 PE=1 SV=2)

HSP 1 Score: 1078.5 bits (2788), Expect = 0.0e+00
Identity = 534/1060 (50.38%), Postives = 720/1060 (67.92%), Query Frame = 0

Query: 7    KSFARRDSLREIEAKVRVLWEENDVFRAEACETPPKVGE-KFFGNFPFPYMNGFLHLGHA 66
            K  A+ D L++IE +++  W+   VF   A     +  + K+F  FP+PYMNG LHLGH 
Sbjct: 5    KGTAKVDFLKKIEKEIQQKWDTERVFEVNASNLEKQTSKGKYFVTFPYPYMNGRLHLGHT 64

Query: 67   FSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPREIEEQ 126
            FSLSK EFA  Y RL+G   L PFG HCTGMPIKA ADKL REI+ +G PP FP E EE+
Sbjct: 65   FSLSKCEFAVGYQRLKGKCCLFPFGLHCTGMPIKACADKLKREIELYGCPPDFPDEEEEE 124

Query: 127  ENLKAEAEDANESNPTLPDKFKGKKSKAASKSSGQMYQWEIMRSFGLSDSEISKFQNPYN 186
            E    + ED       + DK KGKKSKAA+K+    YQW IM+S GLSD EI KF    +
Sbjct: 125  EETSVKTEDI-----IIKDKAKGKKSKAAAKAGSSKYQWGIMKSLGLSDEEIVKFSEAEH 184

Query: 187  WLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKDVRYTI 246
            WL +FPPLA++DLK  GL  DWRRSFITTD+NP++DSFV+WQ   L+   KI    RYTI
Sbjct: 185  WLDYFPPLAIQDLKRMGLKVDWRRSFITTDVNPYYDSFVRWQFLTLRERNKIKFGKRYTI 244

Query: 247  YSPLDGQPCADHDRATGEGVQPQDYTLIKMEVVPPFPPKMGVLVGRKVYLAAATLRPETM 306
            YSP DGQPC DHDR TGEGV PQ+YTL+K++V+ P+P K+  L G+ ++L AATLRPETM
Sbjct: 245  YSPKDGQPCMDHDRQTGEGVGPQEYTLLKLKVLEPYPSKLSGLKGKNIFLVAATLRPETM 304

Query: 307  YGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSKVPEKPTCLIQLTGNDLIG 366
            +GQTN WV P+ KY  FE  + D+FI T++AA N++YQ F+K       + +L G +++G
Sbjct: 305  FGQTNCWVRPDMKYIGFETVNGDIFICTQKAARNMSYQGFTKDNGVVPVVKELMGEEILG 364

Query: 367  LPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRAKYGVK 426
              L +PL   ++IY LPMLTI  DKGTG+VTSVPSD+PDD  A+ DLK K ALRAKYG++
Sbjct: 365  ASLSAPLTSYKVIYVLPMLTIKEDKGTGVVTSVPSDSPDDIAALRDLKKKQALRAKYGIR 424

Query: 427  DEWVLPYDIVPIIDIPEFGDRAAEKVCLDLKIKSQNEKDKLAEAKRLTYLKGFTDGTLIV 486
            D+ VLP++ VP+I+IP FG+ +A  +C +LKI+SQN+++KLAEAK   YLKGF +G ++V
Sbjct: 425  DDMVLPFEPVPVIEIPGFGNLSAVTICDELKIQSQNDREKLAEAKEKIYLKGFYEGIMLV 484

Query: 487  GEFAGRKVQEAKPLIRSKLIETGQAIPYSEPEKRVISRSGDECIVALTDQWYITYGESEW 546
              F G+KVQ+ K  I+ K+I+ G A+ Y EPEK+V+SRS DEC+VAL DQWY+ YGE  W
Sbjct: 485  DGFKGQKVQDVKKTIQKKMIDAGDALIYMEPEKQVMSRSSDECVVALCDQWYLDYGEENW 544

Query: 547  KKLAEECLANMQLFSDETRHGFEHTLSWLNQWACSRSFGLGTRIPWDEQFLVESLSDSTI 606
            KK   +CL N++ F +ETR  FE TL WL + ACSR++GLGT +PWDEQ+L+ESLSDSTI
Sbjct: 545  KKQTSQCLKNLETFCEETRRNFEATLGWLQEHACSRTYGLGTHLPWDEQWLIESLSDSTI 604

Query: 607  YMAYYTIAHLLHNGDLYGSGAS--AIKPEQMTDEVWDFVFCGAAEPKSTGISKSILNKMK 666
            YMA+YT+AHLL  G+L+G   S   I+P+QMT EVWD+VF   A    T I+K  L+++K
Sbjct: 605  YMAFYTVAHLLQGGNLHGQAESPLGIRPQQMTKEVWDYVFFKEAPFPKTQIAKEKLDQLK 664

Query: 667  QEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPRH--HWPRGFRCNGHIMLNSEKMSK 726
            QEFE+WYP DLRVSGKDL+ NHL++ +YNH A+ P     WP   R NGH++LNSEKMSK
Sbjct: 665  QEFEFWYPVDLRVSGKDLVPNHLSYYLYNHVAMWPEQSDKWPTAVRANGHLLLNSEKMSK 724

Query: 727  STGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDILQ 786
            STGNF TL QAI++FSAD  R +LADAGD V+DANFV   A+A ILRL   + W+++++ 
Sbjct: 725  STGNFLTLTQAIDKFSADGMRLALADAGDTVEDANFVEAMADAGILRLYTWVEWVKEMVA 784

Query: 787  ADSASFLRTGPPSTYADRVFENEINIAVKMTEQNYKDYMFREALKTGFYDLQAARDEYRF 846
              +   LR+GP ST+ DRVF +E+N  +  T+QNY+  MF+EALKTGF++ QAA+D+YR 
Sbjct: 785  --NWDSLRSGPASTFNDRVFASELNAGIIKTDQNYEKMMFKEALKTGFFEFQAAKDKYR- 844

Query: 847  SCGAAGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPLADSPDLTL 906
                 GM+R+LVFRF++VQT L+ P CPH  E++W  +L K   ++NA WP+A   +  L
Sbjct: 845  ELAVEGMHRELVFRFIEVQTLLLAPFCPHLCEHIW-TLLGKPDSIMNASWPVAGPVNEVL 904

Query: 907  KSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTTVIEDKKLTGLIYVNEQFDGWKAE 966
              +++YL +    +R  L+  ++ +K       P+      K     IYV + +  W+  
Sbjct: 905  IHSSQYLMEVTHDLRLRLKNYMMPAKGKKTDKQPL-----QKPSHCTIYVAKNYPPWQHT 964

Query: 967  CLRILRSKFDSTKRTFAPDSEIMEALQKSSVGQAVDFRQTQKLCMPFLRFKKDEAAALGV 1026
             L +LR  F++      PD++++     S +G   + ++  K  MPF+   K+    +G 
Sbjct: 965  TLSVLRKHFEANNGKL-PDNKVI----ASELGSMPELKKYMKKVMPFVAMIKENLEKMGP 1024

Query: 1027 HALDLKLPFGEMEVLKENLELIKRQIGLEEVEVLCASDTD 1062
              LDL+L F E  VL EN+  +   + LE +EV  AS+ +
Sbjct: 1025 RILDLQLEFDEKAVLMENIVYLTNSLELEHIEVKFASEAE 1045

BLAST of Spg027529 vs. ExPASy Swiss-Prot
Match: Q8BMJ2 (Leucine--tRNA ligase, cytoplasmic OS=Mus musculus OX=10090 GN=Lars1 PE=1 SV=2)

HSP 1 Score: 1065.1 bits (2753), Expect = 5.4e-310
Identity = 536/1090 (49.17%), Postives = 720/1090 (66.06%), Query Frame = 0

Query: 7    KSFARRDSLREIEAKVRVLWEENDVFRAEACETPPKVGE---KFFGNFPFPYMNGFLHLG 66
            K  A+ D L+EIE + +  WE   VF   A     +      K+F  FP+PYMNG LHLG
Sbjct: 5    KGTAKVDFLKEIEKEAQQKWEAEKVFEVSASRLEKQKQSSKGKYFVTFPYPYMNGRLHLG 64

Query: 67   HAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPREIE 126
            H FSLSK EFA  Y RL+G + L PFG HCTGMPIKA ADKL REI+ +G PP FP E E
Sbjct: 65   HTFSLSKCEFAVGYQRLKGKSCLFPFGLHCTGMPIKACADKLKREIELYGCPPDFPEEEE 124

Query: 127  EQENLKAEAEDANESNPTLPDKFKGKKSKAASKSSGQMYQWEIMRSFGLSDSEISKFQNP 186
            E+E   A+  D       + DK KGKKSKAA+K+    YQW+IM+S GLSD +I KF   
Sbjct: 125  EEEESSAKPGDI-----VVRDKAKGKKSKAAAKAGSSKYQWDIMKSLGLSDDDIVKFSEA 184

Query: 187  YNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKDVRY 246
             +WL +FPPLA++DLK  GL  DWRRSFITTD+NP++DSFV+WQ   L+   KI    RY
Sbjct: 185  EHWLDYFPPLAVQDLKTIGLKVDWRRSFITTDVNPYYDSFVRWQFLTLRERNKIKFGKRY 244

Query: 247  TIYSPLDGQPCADHDRATGEGVQPQDYTLIKMEVVPPFPPKMGVLVGRKVYLAAATLRPE 306
            TIYSP DGQPC DHDR TGEGV PQ+YTL+K++V+ P+P K+  L G+ ++L AATLRPE
Sbjct: 245  TIYSPKDGQPCMDHDRQTGEGVGPQEYTLVKLKVLEPYPSKLSGLKGKNIFLVAATLRPE 304

Query: 307  TMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSKVPEKPTCLIQLTGNDL 366
            TM+GQTN WV P+ KY  FE  + D+FI T+RAA N++YQ F+K       + +L G ++
Sbjct: 305  TMFGQTNCWVRPDMKYIGFETANGDIFICTQRAARNMSYQGFTKHNGVVPVVKELMGEEI 364

Query: 367  IGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRAKYG 426
            +G  L +PL   +++Y LPMLTI  DKGTG+VTSVPSD+PDD  A+ DLK K ALR K+G
Sbjct: 365  LGASLSAPLTCYKVVYVLPMLTIKEDKGTGVVTSVPSDSPDDLAALRDLKKKQALRTKFG 424

Query: 427  VKDEWVLPYDIVPIIDIPEFGDRAAEKVCLDLKIKSQNEKDKLAEAKRLTYLKGFTDGTL 486
            ++D+ VLP++ VP+++IP  G+  A  VC +LKI+SQN+++KLAEAK   YL+GF DG +
Sbjct: 425  IRDDMVLPFEPVPVLEIPGIGNLPAVTVCDELKIQSQNDREKLAEAKEKLYLRGFYDGVM 484

Query: 487  IVGEFAGRKVQEAKPLIRSKLIETGQAIPYSEPEKRVISRSGDECIVALTDQWYITYGES 546
            +V  F G+K+Q  K  I+  +I+ G A+ Y EPEK+V+SRS DEC+VAL DQWY+ YG+ 
Sbjct: 485  LVDGFKGQKIQHVKKTIQKNMIDAGDALIYMEPEKQVMSRSADECVVALCDQWYLDYGDE 544

Query: 547  EWKKLAEECLANMQLFSDETRHGFEHTLSWLNQWACSRSFGLGTRIPWDEQFLVESLSDS 606
             WKK   +CL NM+ F +E+R  FE +L WL + ACSR++GLGTR+PWDEQ+L+ESLSDS
Sbjct: 545  NWKKQTFQCLKNMETFCEESRKNFEASLDWLQEHACSRTYGLGTRLPWDEQWLIESLSDS 604

Query: 607  TIYMAYYTIAHLLHNGDLYGSGAS--AIKPEQMTDEVWDFVFCGAAEPKSTGISKSILNK 666
            TIYMA+YT+AHLL  GDL G   S   I+P+QMT +VWD+VF   A    T I K  L++
Sbjct: 605  TIYMAFYTVAHLLQGGDLNGQAESPLGIRPQQMTKDVWDYVFFKDAPFPKTQIPKEKLDQ 664

Query: 667  MKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPRH--HWPRGFRCNGHIMLNSEKM 726
            +KQEFE+WYP DLR SGKDLI NHL++ IYNH A+ P     WP   R NGH++LNSEKM
Sbjct: 665  LKQEFEFWYPVDLRASGKDLIPNHLSYYIYNHVAMWPEQSDKWPVSVRANGHLLLNSEKM 724

Query: 727  SKSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDI 786
            SKSTGNF TL QA+++FSAD  R +LADAGD V+DANFV   A+A ILRL   + W++++
Sbjct: 725  SKSTGNFLTLSQAVDKFSADGMRLALADAGDTVEDANFVEAMADAGILRLYTWVEWVKEM 784

Query: 787  LQADSASFLRTGPPSTYADRVFENEINIAVKMTEQNYKDYMFREALKTGFYDLQAARDEY 846
            L   S S LR+GP  ++ DRVF +E+N  +  T+QNY+  MF+EALKTGF++ QAA+D+Y
Sbjct: 785  LA--SCSSLRSGPADSFNDRVFASEMNAGIIKTDQNYEKMMFKEALKTGFFEFQAAKDKY 844

Query: 847  RFSCGAAGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPLADSPDL 906
            R      GM+R+LVFRF++VQT L+TP CPH  E++W  +L K   +++A WP+A   D 
Sbjct: 845  R-ELATEGMHRELVFRFIEVQTILLTPFCPHLCEHIW-TLLGKPDSIMHASWPVAGPVDE 904

Query: 907  TLKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTTVIEDKKLTGLIYVNEQFDGWK 966
            +L  +++YL +    +R  L+  ++ +K       P       +     IYV + +  W+
Sbjct: 905  SLIRSSQYLMEVAHDLRLRLKNYMMPAKGKKTDKQPA-----QRPSHCTIYVAKNYPVWQ 964

Query: 967  AECLRILRSKFDSTKRTFAPDSEIMEALQKSSVGQAVDFRQTQKLCMPFLRFKKDEAAAL 1026
               L  LRS F++      PD++++     S +G   + ++  K  MPF+   K+     
Sbjct: 965  HITLTTLRSHFEANNGKL-PDNKVI----ASELGSLPELKKYMKKVMPFVAMIKENMEKK 1024

Query: 1027 GVHALDLKLPFGEMEVLKENLELIKRQIGLEEVEVLCASDTDALTKAGALASLLKQNPPS 1086
            G   LDL+L F E  VL EN+  +   + LE +EV  AS+ +   +              
Sbjct: 1025 GPRVLDLELEFDEQAVLMENIVYLTNSLELEHIEVKFASEAEDKVREEC----------C 1065

Query: 1087 PGNPTAIFLT 1090
            PG P  +F T
Sbjct: 1085 PGKPLNVFRT 1065

BLAST of Spg027529 vs. ExPASy Swiss-Prot
Match: Q09996 (Leucine--tRNA ligase OS=Caenorhabditis elegans OX=6239 GN=lars-1 PE=3 SV=2)

HSP 1 Score: 1019.2 bits (2634), Expect = 3.4e-296
Identity = 519/1064 (48.78%), Postives = 698/1064 (65.60%), Query Frame = 0

Query: 3    SESGKSFARRDSLREIEAKVRVLWEENDVFRAEACETPPKVGEKFFGNFPFPYMNGFLHL 62
            S+  K   +   L+EIE  ++ LWE    F A+A +       K+   FPFPYMNG LHL
Sbjct: 2    SKINKERKKVAQLQEIEKSIQELWESKKAFEADARDDGK---PKYLVTFPFPYMNGRLHL 61

Query: 63   GHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPREI 122
            GH FS SK EFAA + RL+G  VL PFGFHCTGMPIKA ADKL RE+Q FG PP FP ++
Sbjct: 62   GHTFSASKCEFAAGFQRLQGKEVLFPFGFHCTGMPIKACADKLKREMQDFGYPPNFPEDV 121

Query: 123  EEQENLKAEAEDANESNPTLPDKFKGKKSKAASKSSGQMYQWEIMRSFGLSDSEISKFQN 182
             E+       E+ +  +  + DK KGKKSK  +K+    YQW+IM+S GL D EI +F +
Sbjct: 122  VEEVK-----EEVSAVDEIIKDKSKGKKSKLVAKTGNAKYQWQIMKSLGLCDEEIKEFSD 181

Query: 183  PYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKDVR 242
            P +WL +FPP  + DLK  GL  DWRRSFITTD+NP+FDSFV+WQ   L++  KI    R
Sbjct: 182  PNHWLYYFPPHCIADLKKMGLKADWRRSFITTDVNPYFDSFVRWQFNLLRAAKKIDFGKR 241

Query: 243  YTIYSPLDGQPCADHDRATGEGVQPQDYTLIKMEVVPPFPPKMGVLVGRKVYLAAATLRP 302
            YTIYSP DGQPC DHDRA+GEGV PQ+YTLIK++V+ P P  +   +   +YL AATLRP
Sbjct: 242  YTIYSPKDGQPCMDHDRASGEGVGPQEYTLIKLKVLDPKPQALS-HIKEDIYLVAATLRP 301

Query: 303  ETMYGQTNAWVLPEGKYGAFEI--NDTDVFIITERAALNLAYQKFSKVPEKPTCLI---Q 362
            ETMYGQTN ++ P+ +Y  F    N+  VF+ T R+A  ++YQ  +K   K   ++   +
Sbjct: 302  ETMYGQTNCYLHPDIQYSVFYATENEKQVFVATARSARIMSYQGLTKENGKVNYVLGLEK 361

Query: 363  LTGNDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPA 422
            + G+ ++G PL +PLA  E +YALPMLTI  DKGTG+VTSVPSD+PDD+ A+ DLK K  
Sbjct: 362  IAGSKILGAPLSAPLAKYERVYALPMLTIKDDKGTGVVTSVPSDSPDDFAALSDLKKKKP 421

Query: 423  LRAKYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCLDLKIKSQNEKDKLAEAKRLTYLKG 482
            LR KYG+ DE VLP++ VPII I   GD AA ++C  LKI+SQNEKDKL EAK+  YLKG
Sbjct: 422  LREKYGLTDEMVLPFEPVPIIKIEGLGDLAAVEMCSRLKIESQNEKDKLEEAKKEVYLKG 481

Query: 483  FTDGTLIVGEFAGRKVQEAKPLIRSKLIETGQAIPYSEPEKRVISRSGDECIVALTDQWY 542
            F DG ++VG++AG+K  + K +I+  L   G A  Y EPEK+V+SRSGDEC+VAL DQWY
Sbjct: 482  FYDGVMLVGKYAGKKTADVKKVIQDDLTAEGLATKYVEPEKKVMSRSGDECVVALCDQWY 541

Query: 543  ITYGESEWKKLAEECLANMQLFSDETRHGFEHTLSWLNQWACSRSFGLGTRIPWDEQFLV 602
            + YGE+EWK  A++ L  ++ F+DETR G E T+ WL+++ACSRS+GLGT++PWD Q+L+
Sbjct: 542  LNYGEAEWKAAAKKVLEPLRTFNDETRRGLETTVDWLHEYACSRSYGLGTKLPWDTQYLI 601

Query: 603  ESLSDSTIYMAYYTIAHLLHNGDLYGS--GASAIKPEQMTDEVWDFVFCGAA-EPKSTGI 662
            ESLSDSTIY AYYT+AHLL  G   GS  G + IK +QMTD  W +VF G   + K+  +
Sbjct: 602  ESLSDSTIYNAYYTVAHLLQQGAFDGSVVGPAGIKADQMTDASWSYVFLGEIYDSKTMPV 661

Query: 663  SKSILNKMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMP--RHHWPRGFRCNGHI 722
             +  L  +++EF YWYP D+R SGKDLI NHLT+ ++NH AI P     WP+G R NGH+
Sbjct: 662  EEEKLKSLRKEFMYWYPIDMRASGKDLIGNHLTYLLFNHAAIWPTDTSKWPKGIRANGHL 721

Query: 723  MLNSEKMSKSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKE 782
            +LN+EKMSKSTGNF TL +AIE+FSAD  R SLADAGDG++DANFV+  A+AAILRL   
Sbjct: 722  LLNNEKMSKSTGNFMTLEEAIEKFSADGMRLSLADAGDGLEDANFVYAMADAAILRLFTM 781

Query: 783  IAWMEDILQADSASFLRTGPPSTYADRVFENEINIAVKMTEQNYKDYMFREALKTGFYDL 842
            I W++++++   A  LR      +ADRVF NE+N  +  TEQNY+   F++ALKTGF++ 
Sbjct: 782  IEWIKEMIEQRDAGLLRK-DAKKFADRVFANEMNSLIAATEQNYEATNFKDALKTGFFEY 841

Query: 843  QAARDEYRFSCGAAG--MNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAG 902
            QA RD YR  C      M+  LVFRF++ Q  +++PICPH AEY+W ++L K+G +VNA 
Sbjct: 842  QAIRDTYRELCAGIDEPMSESLVFRFIETQMLILSPICPHIAEYIW-QLLGKDGLIVNAP 901

Query: 903  WPLADSPDLTLKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTTVIEDKKLTGLIY 962
            WP  D  D  L    +++ +S+   R  L+  +   KK  K+   V T         +IY
Sbjct: 902  WPTVDPVDEKLAIGARFITESLAEFRARLKTYMTPKKKALKEIPEVPT-------EAVIY 961

Query: 963  VNEQFDGWKAECLRILRSKFDSTKRTFAPDSEIMEALQKSSVGQAVDFRQTQKLCMPFLR 1022
            V +++  W+   L IL  +  +      PD++ +  L    +G+    ++  K  MPF++
Sbjct: 962  VAKEYPPWQKTILDILEKQAKANNGAL-PDNKAISQL----IGKEESLKKFAKKAMPFVQ 1021

Query: 1023 FKKDEAAALGVHALDLKLPFGEMEVLKENLELIKRQIGLEEVEV 1055
              K+     GV AL    P  +  +L EN++ I   + L+ V +
Sbjct: 1022 MIKERFEQKGVSALASSSPVDQTSILNENIDFIMNALDLDRVTI 1042

BLAST of Spg027529 vs. ExPASy TrEMBL
Match: A0A6J1G9C6 (Leucyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111451993 PE=3 SV=1)

HSP 1 Score: 2173.7 bits (5631), Expect = 0.0e+00
Identity = 1058/1090 (97.06%), Postives = 1075/1090 (98.62%), Query Frame = 0

Query: 1    MASESGKSFARRDSLREIEAKVRVLWEENDVFRAEACETPPKVGEKFFGNFPFPYMNGFL 60
            MASE GKSFARRDSLREIE+KVRVLWEENDVFRAEACETPPK GEKFFGNFPFPYMNGFL
Sbjct: 1    MASECGKSFARRDSLREIESKVRVLWEENDVFRAEACETPPKEGEKFFGNFPFPYMNGFL 60

Query: 61   HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR 120
            HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR
Sbjct: 61   HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR 120

Query: 121  EIEEQENLKAEAEDANESNPTLPDKFKGKKSKAASKSSGQMYQWEIMRSFGLSDSEISKF 180
            ++EEQENLKAE+EDANESNPTLPDKFKGKKSKA SKSSGQMYQWEIMRSFGLSDSEISKF
Sbjct: 121  QMEEQENLKAESEDANESNPTLPDKFKGKKSKAVSKSSGQMYQWEIMRSFGLSDSEISKF 180

Query: 181  QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD 240
            QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD
Sbjct: 181  QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD 240

Query: 241  VRYTIYSPLDGQPCADHDRATGEGVQPQDYTLIKMEVVPPFPPKMGVLVGRKVYLAAATL 300
            VRYTIYSPLDGQPCADHDRA+GEGVQPQDYTLIKMEVVPPFPPKMGVL GRKVYLAAATL
Sbjct: 241  VRYTIYSPLDGQPCADHDRASGEGVQPQDYTLIKMEVVPPFPPKMGVLEGRKVYLAAATL 300

Query: 301  RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSKVPEKPTCLIQLTG 360
            RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFS+VPEKPTCLIQLTG
Sbjct: 301  RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSRVPEKPTCLIQLTG 360

Query: 361  NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 420
            NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA
Sbjct: 361  NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 420

Query: 421  KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCLDLKIKSQNEKDKLAEAKRLTYLKGFTD 480
            KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVC+DLKIKSQNEKDKLAEAKRLTYLKGFTD
Sbjct: 421  KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTD 480

Query: 481  GTLIVGEFAGRKVQEAKPLIRSKLIETGQAIPYSEPEKRVISRSGDECIVALTDQWYITY 540
            GTLIVGEF+GRKVQEAKP+IRSKLIETGQAIPYSEPEKRV SRSGDECIVALTDQWYITY
Sbjct: 481  GTLIVGEFSGRKVQEAKPMIRSKLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITY 540

Query: 541  GESEWKKLAEECLANMQLFSDETRHGFEHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 600
            GESEWKKLAE+CLANM LFSDETRHGF+HTLSWLNQWACSRSFGLGTRIPWDEQFLVESL
Sbjct: 541  GESEWKKLAEDCLANMNLFSDETRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 600

Query: 601  SDSTIYMAYYTIAHLLHNGDLYGSGASAIKPEQMTDEVWDFVFCGAAEPKSTGISKSILN 660
            SDSTIYMAYYTIAHLLHNGDLYGSGASAIKPEQMTDEVWDFVFCGAAEPKSTGIS+SILN
Sbjct: 601  SDSTIYMAYYTIAHLLHNGDLYGSGASAIKPEQMTDEVWDFVFCGAAEPKSTGISRSILN 660

Query: 661  KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPRHHWPRGFRCNGHIMLNSEKMS 720
            KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMP HHWPRGFRCNGHIMLNSEKMS
Sbjct: 661  KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPEHHWPRGFRCNGHIMLNSEKMS 720

Query: 721  KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 780
            KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL
Sbjct: 721  KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 780

Query: 781  QADSASFLRTGPPSTYADRVFENEINIAVKMTEQNYKDYMFREALKTGFYDLQAARDEYR 840
            QADS+SFLRTGPPSTYADRVFENEINIAVK TEQNYKDYMFREALK+GFYDLQAARDEYR
Sbjct: 781  QADSSSFLRTGPPSTYADRVFENEINIAVKTTEQNYKDYMFREALKSGFYDLQAARDEYR 840

Query: 841  FSCGAAGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPLADSPDLT 900
            FSCGA GMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWP A SPDLT
Sbjct: 841  FSCGAGGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPSAGSPDLT 900

Query: 901  LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTTVIEDKKLTGLIYVNEQFDGWKA 960
            LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVT+VIEDKKLTGLIYVNEQFDGWKA
Sbjct: 901  LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTSVIEDKKLTGLIYVNEQFDGWKA 960

Query: 961  ECLRILRSKFDSTKRTFAPDSEIMEALQKSSVGQAVDFRQTQKLCMPFLRFKKDEAAALG 1020
            ECLRILR KFDSTKRTFAPDSEIMEALQKSSVGQA DFRQTQKLCMPFLRFKKDE AALG
Sbjct: 961  ECLRILRCKFDSTKRTFAPDSEIMEALQKSSVGQASDFRQTQKLCMPFLRFKKDEVAALG 1020

Query: 1021 VHALDLKLPFGEMEVLKENLELIKRQIGLEEVEVLCASDTDALTKAGALASLLKQNPPSP 1080
            VHALDLKLPFGE+EVLKENLELIKRQIGLEEVEVLCASDTDA+ KAGALASLLKQNPPSP
Sbjct: 1021 VHALDLKLPFGEIEVLKENLELIKRQIGLEEVEVLCASDTDAIAKAGALASLLKQNPPSP 1080

Query: 1081 GNPTAIFLTS 1091
            GNPTAIFLTS
Sbjct: 1081 GNPTAIFLTS 1090

BLAST of Spg027529 vs. ExPASy TrEMBL
Match: A0A6J1KFQ2 (Leucyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111493448 PE=3 SV=1)

HSP 1 Score: 2149.4 bits (5568), Expect = 0.0e+00
Identity = 1046/1090 (95.96%), Postives = 1070/1090 (98.17%), Query Frame = 0

Query: 1    MASESGKSFARRDSLREIEAKVRVLWEENDVFRAEACETPPKVGEKFFGNFPFPYMNGFL 60
            M SE GKSFARRDSLREIE+KVRVLWEENDVFRAEACETPPK GEKFFGNFPFPYMNGFL
Sbjct: 1    MTSECGKSFARRDSLREIESKVRVLWEENDVFRAEACETPPKEGEKFFGNFPFPYMNGFL 60

Query: 61   HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR 120
            HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREI+QFGDPPVFP 
Sbjct: 61   HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIKQFGDPPVFPS 120

Query: 121  EIEEQENLKAEAEDANESNPTLPDKFKGKKSKAASKSSGQMYQWEIMRSFGLSDSEISKF 180
            +IEE+ENLKAE+EDANESNPTLPDKFKGKKSKA SKSSGQMYQWEIMRSFGLSDSEISKF
Sbjct: 121  QIEEEENLKAESEDANESNPTLPDKFKGKKSKAVSKSSGQMYQWEIMRSFGLSDSEISKF 180

Query: 181  QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD 240
            QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD
Sbjct: 181  QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD 240

Query: 241  VRYTIYSPLDGQPCADHDRATGEGVQPQDYTLIKMEVVPPFPPKMGVLVGRKVYLAAATL 300
            VRYTIYSPLDGQPCADHDRA+GEGVQPQDYTLIKMEVVPPFPPKM VL GRKVYLAAATL
Sbjct: 241  VRYTIYSPLDGQPCADHDRASGEGVQPQDYTLIKMEVVPPFPPKMSVLEGRKVYLAAATL 300

Query: 301  RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSKVPEKPTCLIQLTG 360
            RPETMYGQTNAWVLPEGKYGAFEINDTDV+IITERAALNLAYQKFS+VPEKPTCLIQLTG
Sbjct: 301  RPETMYGQTNAWVLPEGKYGAFEINDTDVYIITERAALNLAYQKFSRVPEKPTCLIQLTG 360

Query: 361  NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 420
            NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA
Sbjct: 361  NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 420

Query: 421  KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCLDLKIKSQNEKDKLAEAKRLTYLKGFTD 480
            KYGVKDEWVLPYDIVPII+IPEFGDRAAEKVC+DLKIKSQNEKDKLAEAKRLTYLKGFTD
Sbjct: 421  KYGVKDEWVLPYDIVPIINIPEFGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTD 480

Query: 481  GTLIVGEFAGRKVQEAKPLIRSKLIETGQAIPYSEPEKRVISRSGDECIVALTDQWYITY 540
            GTLIVGEF+GRKVQEAKP+IRSKLIETGQAIPYSEPEKRVISRSGDECIVALTDQWYITY
Sbjct: 481  GTLIVGEFSGRKVQEAKPMIRSKLIETGQAIPYSEPEKRVISRSGDECIVALTDQWYITY 540

Query: 541  GESEWKKLAEECLANMQLFSDETRHGFEHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 600
            GESEWKKLAE+CLANM LFSDETRHGF+HTLSWLNQWACSRSFGLGTRIPWDEQFLVESL
Sbjct: 541  GESEWKKLAEDCLANMNLFSDETRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 600

Query: 601  SDSTIYMAYYTIAHLLHNGDLYGSGASAIKPEQMTDEVWDFVFCGAAEPKSTGISKSILN 660
            SDSTIYMAYYTIAHLLH+GDLYGSGASAIKPEQMTDEVWDFVF GAAEPKSTGIS+SILN
Sbjct: 601  SDSTIYMAYYTIAHLLHSGDLYGSGASAIKPEQMTDEVWDFVFFGAAEPKSTGISRSILN 660

Query: 661  KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPRHHWPRGFRCNGHIMLNSEKMS 720
            KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMP HHWPRGFRCNGHIMLNSEKMS
Sbjct: 661  KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPEHHWPRGFRCNGHIMLNSEKMS 720

Query: 721  KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 780
            KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL
Sbjct: 721  KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 780

Query: 781  QADSASFLRTGPPSTYADRVFENEINIAVKMTEQNYKDYMFREALKTGFYDLQAARDEYR 840
            QADS+SFLRTGPPSTYADRVFENE+NIAVK TEQNYK+YMFREALK+GFYDLQAARDEYR
Sbjct: 781  QADSSSFLRTGPPSTYADRVFENEMNIAVKTTEQNYKNYMFREALKSGFYDLQAARDEYR 840

Query: 841  FSCGAAGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPLADSPDLT 900
            FSCGA GMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWP A  PDLT
Sbjct: 841  FSCGAGGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPSAGYPDLT 900

Query: 901  LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTTVIEDKKLTGLIYVNEQFDGWKA 960
            LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVT VIEDKKLTGLIYVNEQFDGWKA
Sbjct: 901  LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTYVIEDKKLTGLIYVNEQFDGWKA 960

Query: 961  ECLRILRSKFDSTKRTFAPDSEIMEALQKSSVGQAVDFRQTQKLCMPFLRFKKDEAAALG 1020
            ECLRIL+ KFDSTKRTFAPDSEIMEALQKSSVGQA DFRQTQKLCMPFLRFKKDE AALG
Sbjct: 961  ECLRILQCKFDSTKRTFAPDSEIMEALQKSSVGQASDFRQTQKLCMPFLRFKKDEVAALG 1020

Query: 1021 VHALDLKLPFGEMEVLKENLELIKRQIGLEEVEVLCASDTDALTKAGALASLLKQNPPSP 1080
            VHALDLKLPFGE+EVLKENLELIKRQIGLEEVEVLCASDTDA+ K+GALASLLKQNPPSP
Sbjct: 1021 VHALDLKLPFGEIEVLKENLELIKRQIGLEEVEVLCASDTDAIAKSGALASLLKQNPPSP 1080

Query: 1081 GNPTAIFLTS 1091
            GNPTAIFLTS
Sbjct: 1081 GNPTAIFLTS 1090

BLAST of Spg027529 vs. ExPASy TrEMBL
Match: A0A6J1I0H8 (Leucyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111469399 PE=3 SV=1)

HSP 1 Score: 2137.8 bits (5538), Expect = 0.0e+00
Identity = 1037/1090 (95.14%), Postives = 1066/1090 (97.80%), Query Frame = 0

Query: 1    MASESGKSFARRDSLREIEAKVRVLWEENDVFRAEACETPPKVGEKFFGNFPFPYMNGFL 60
            MASESGKSFARRDSLR+IEAKVRVLWEENDVFRAEACETPPKVGEKFFGNFPFPYMNGFL
Sbjct: 1    MASESGKSFARRDSLRDIEAKVRVLWEENDVFRAEACETPPKVGEKFFGNFPFPYMNGFL 60

Query: 61   HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR 120
            HLGHAFS+SKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFP 
Sbjct: 61   HLGHAFSVSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPS 120

Query: 121  EIEEQENLKAEAEDANESNPTLPDKFKGKKSKAASKSSGQMYQWEIMRSFGLSDSEISKF 180
            + EEQENLKAEAED NESNPTLPDKFKGKKSKAASKSSGQMYQWEIMRSFGLSDSEISKF
Sbjct: 121  QTEEQENLKAEAEDTNESNPTLPDKFKGKKSKAASKSSGQMYQWEIMRSFGLSDSEISKF 180

Query: 181  QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD 240
            QNPYNWLTFFP +AMEDLKAFGLGCDWRRSFITTDMNPFF+SFVQWQMRKLKSMGKIVKD
Sbjct: 181  QNPYNWLTFFPTVAMEDLKAFGLGCDWRRSFITTDMNPFFNSFVQWQMRKLKSMGKIVKD 240

Query: 241  VRYTIYSPLDGQPCADHDRATGEGVQPQDYTLIKMEVVPPFPPKMGVLVGRKVYLAAATL 300
            VRY IYS LDGQPCADHDRA+GEGV PQDYTLIKMEVV PFPPK+GVL GRKV+LAAATL
Sbjct: 241  VRYAIYSTLDGQPCADHDRASGEGVLPQDYTLIKMEVVAPFPPKLGVLEGRKVFLAAATL 300

Query: 301  RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSKVPEKPTCLIQLTG 360
            RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFS++PEKPTCLIQLTG
Sbjct: 301  RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSRIPEKPTCLIQLTG 360

Query: 361  NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 420
            NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA
Sbjct: 361  NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 420

Query: 421  KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCLDLKIKSQNEKDKLAEAKRLTYLKGFTD 480
            KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCLDLKIKSQNEKDKLAEAKRLTYLKGFTD
Sbjct: 421  KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCLDLKIKSQNEKDKLAEAKRLTYLKGFTD 480

Query: 481  GTLIVGEFAGRKVQEAKPLIRSKLIETGQAIPYSEPEKRVISRSGDECIVALTDQWYITY 540
            GTLIVGEFAGRKVQEAKPLIRSKLIETG+AIPYSEPEKRVISRSGDECIVALTDQWYITY
Sbjct: 481  GTLIVGEFAGRKVQEAKPLIRSKLIETGEAIPYSEPEKRVISRSGDECIVALTDQWYITY 540

Query: 541  GESEWKKLAEECLANMQLFSDETRHGFEHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 600
            GESEWKKLAEECLANM LFSDETRHGFEHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL
Sbjct: 541  GESEWKKLAEECLANMDLFSDETRHGFEHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 600

Query: 601  SDSTIYMAYYTIAHLLHNGDLYGSGASAIKPEQMTDEVWDFVFCGAAEPKSTGISKSILN 660
            SDSTIYMAYYTIAH LHNGDLY S ASAIKPEQMTDEVWDF+FCGAAEPKSTGIS+S+LN
Sbjct: 601  SDSTIYMAYYTIAHFLHNGDLYASDASAIKPEQMTDEVWDFIFCGAAEPKSTGISQSVLN 660

Query: 661  KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPRHHWPRGFRCNGHIMLNSEKMS 720
            KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPRHHWPRGFRCNGHIMLNSEKMS
Sbjct: 661  KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPRHHWPRGFRCNGHIMLNSEKMS 720

Query: 721  KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 780
            KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL
Sbjct: 721  KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 780

Query: 781  QADSASFLRTGPPSTYADRVFENEINIAVKMTEQNYKDYMFREALKTGFYDLQAARDEYR 840
            QADSASFLR GPPSTYADRVFENEINIAV+ TE+NYK+YMFREALKTGFYDLQAARDEYR
Sbjct: 781  QADSASFLRIGPPSTYADRVFENEINIAVRTTEKNYKEYMFREALKTGFYDLQAARDEYR 840

Query: 841  FSCGAAGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPLADSPDLT 900
            FSCGA GMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFV+N+GWPLADSPDLT
Sbjct: 841  FSCGAGGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVINSGWPLADSPDLT 900

Query: 901  LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTTVIEDKKLTGLIYVNEQFDGWKA 960
            LK+ANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVT VIEDKKLTGL+YVNEQFDGWKA
Sbjct: 901  LKTANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTMVIEDKKLTGLVYVNEQFDGWKA 960

Query: 961  ECLRILRSKFDSTKRTFAPDSEIMEALQKSSVGQAVDFRQTQKLCMPFLRFKKDEAAALG 1020
            ECLRILRSKFD+T+RTFAPDSEIMEALQ SSVGQA DF++TQK CMPFLRFKKDEAAALG
Sbjct: 961  ECLRILRSKFDTTRRTFAPDSEIMEALQNSSVGQAADFKKTQKQCMPFLRFKKDEAAALG 1020

Query: 1021 VHALDLKLPFGEMEVLKENLELIKRQIGLEEVEVLCASDTDALTKAGALASLLKQNPPSP 1080
            V ALDLKLPFGE++VL+ENLELIKRQIGLEEVE+LCASD DAL KAGALASLL QNPPSP
Sbjct: 1021 VQALDLKLPFGEIDVLRENLELIKRQIGLEEVEILCASDPDALAKAGALASLLTQNPPSP 1080

Query: 1081 GNPTAIFLTS 1091
            GNPTAIFLTS
Sbjct: 1081 GNPTAIFLTS 1090

BLAST of Spg027529 vs. ExPASy TrEMBL
Match: A0A6J1HLL7 (Leucyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111464710 PE=3 SV=1)

HSP 1 Score: 2134.4 bits (5529), Expect = 0.0e+00
Identity = 1035/1090 (94.95%), Postives = 1064/1090 (97.61%), Query Frame = 0

Query: 1    MASESGKSFARRDSLREIEAKVRVLWEENDVFRAEACETPPKVGEKFFGNFPFPYMNGFL 60
            MASESGKSFARRDSLR+IEAKVRVLWEENDVFRAEACETPPK GEKFFGNFPFPYMNGFL
Sbjct: 1    MASESGKSFARRDSLRDIEAKVRVLWEENDVFRAEACETPPKAGEKFFGNFPFPYMNGFL 60

Query: 61   HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR 120
            HLGHAFS+SKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFP 
Sbjct: 61   HLGHAFSVSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPS 120

Query: 121  EIEEQENLKAEAEDANESNPTLPDKFKGKKSKAASKSSGQMYQWEIMRSFGLSDSEISKF 180
            + EEQENLKAEAED NESNPTLPDKFKGKKSKAASKSSGQMYQWEIMRSFGLSDSEISKF
Sbjct: 121  QTEEQENLKAEAEDTNESNPTLPDKFKGKKSKAASKSSGQMYQWEIMRSFGLSDSEISKF 180

Query: 181  QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD 240
            QNPYNWLTFFPP+AMEDLKAFGLGCDWRRSFITTDMNPFF+SFVQWQMRKLKSMGKIVKD
Sbjct: 181  QNPYNWLTFFPPVAMEDLKAFGLGCDWRRSFITTDMNPFFNSFVQWQMRKLKSMGKIVKD 240

Query: 241  VRYTIYSPLDGQPCADHDRATGEGVQPQDYTLIKMEVVPPFPPKMGVLVGRKVYLAAATL 300
            VRY IYS LDGQPCADHDRA+GEGV PQDYTLIKMEVV PFPPK+GVL GRKV+LAAATL
Sbjct: 241  VRYAIYSTLDGQPCADHDRASGEGVLPQDYTLIKMEVVAPFPPKLGVLEGRKVFLAAATL 300

Query: 301  RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSKVPEKPTCLIQLTG 360
            RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQ+FS++PEKPTCLIQLTG
Sbjct: 301  RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQRFSRIPEKPTCLIQLTG 360

Query: 361  NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 420
            NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA
Sbjct: 361  NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 420

Query: 421  KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCLDLKIKSQNEKDKLAEAKRLTYLKGFTD 480
            KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCLDLKIKSQNEKDKLAEAKRLTYLKGFTD
Sbjct: 421  KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCLDLKIKSQNEKDKLAEAKRLTYLKGFTD 480

Query: 481  GTLIVGEFAGRKVQEAKPLIRSKLIETGQAIPYSEPEKRVISRSGDECIVALTDQWYITY 540
            GTLIVGEFAGRKVQEAKPLIRSKLIETG+AIPYSEPEKRVISRSGDECIVALTDQWYITY
Sbjct: 481  GTLIVGEFAGRKVQEAKPLIRSKLIETGEAIPYSEPEKRVISRSGDECIVALTDQWYITY 540

Query: 541  GESEWKKLAEECLANMQLFSDETRHGFEHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 600
            GESEWKKLAEECLANM LFSDETRHGFEHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL
Sbjct: 541  GESEWKKLAEECLANMDLFSDETRHGFEHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 600

Query: 601  SDSTIYMAYYTIAHLLHNGDLYGSGASAIKPEQMTDEVWDFVFCGAAEPKSTGISKSILN 660
            SDSTIYMAYYTIAH LHNGDLY S ASAIKPEQMTDEVWDF+FCGAAEPKSTGIS+S+LN
Sbjct: 601  SDSTIYMAYYTIAHFLHNGDLYASDASAIKPEQMTDEVWDFIFCGAAEPKSTGISQSVLN 660

Query: 661  KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPRHHWPRGFRCNGHIMLNSEKMS 720
            KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNH AIMPRHHWPRGFRCNGHIMLNSEKMS
Sbjct: 661  KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHAAIMPRHHWPRGFRCNGHIMLNSEKMS 720

Query: 721  KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 780
            KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL
Sbjct: 721  KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 780

Query: 781  QADSASFLRTGPPSTYADRVFENEINIAVKMTEQNYKDYMFREALKTGFYDLQAARDEYR 840
            QADSASFLRTGPPSTYADRVFENEINIAV+ TE+NYKDYMFREALKTGFYDLQAARDEYR
Sbjct: 781  QADSASFLRTGPPSTYADRVFENEINIAVRTTEKNYKDYMFREALKTGFYDLQAARDEYR 840

Query: 841  FSCGAAGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPLADSPDLT 900
            FSCGA GMNRDLVFRFM+VQTRLITPICPHYAEYVWREMLKKEGFV+N+GWPLADSPDLT
Sbjct: 841  FSCGAGGMNRDLVFRFMNVQTRLITPICPHYAEYVWREMLKKEGFVINSGWPLADSPDLT 900

Query: 901  LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTTVIEDKKLTGLIYVNEQFDGWKA 960
            LK ANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVT VIEDKKLTGL+YVNEQFDGWKA
Sbjct: 901  LKIANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTMVIEDKKLTGLVYVNEQFDGWKA 960

Query: 961  ECLRILRSKFDSTKRTFAPDSEIMEALQKSSVGQAVDFRQTQKLCMPFLRFKKDEAAALG 1020
            ECLRILRSKFD+T+RTFAPDSEIMEALQ SSVGQA DF++TQK CMPFLRFKKDEAAALG
Sbjct: 961  ECLRILRSKFDTTRRTFAPDSEIMEALQNSSVGQAADFKKTQKQCMPFLRFKKDEAAALG 1020

Query: 1021 VHALDLKLPFGEMEVLKENLELIKRQIGLEEVEVLCASDTDALTKAGALASLLKQNPPSP 1080
            V AL LKLPFGEM+VL+ENLELIKRQIGLEEVE+LCASD DA+ KAGALASLL QNPPSP
Sbjct: 1021 VQALALKLPFGEMDVLRENLELIKRQIGLEEVEILCASDPDAVAKAGALASLLTQNPPSP 1080

Query: 1081 GNPTAIFLTS 1091
            GNPTAIFLTS
Sbjct: 1081 GNPTAIFLTS 1090

BLAST of Spg027529 vs. ExPASy TrEMBL
Match: A0A6J1DSM3 (Leucyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111023537 PE=3 SV=1)

HSP 1 Score: 2127.1 bits (5510), Expect = 0.0e+00
Identity = 1035/1090 (94.95%), Postives = 1063/1090 (97.52%), Query Frame = 0

Query: 1    MASESGKSFARRDSLREIEAKVRVLWEENDVFRAEACETPPKVGEKFFGNFPFPYMNGFL 60
            MASESGKSFARRD+LREIEAKVR LWEE DVFRAEA ETPPKVGEKFFGNFPFPYMNGFL
Sbjct: 1    MASESGKSFARRDNLREIEAKVRALWEEYDVFRAEALETPPKVGEKFFGNFPFPYMNGFL 60

Query: 61   HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR 120
            H+GHAFS+SKLEFAAAYHRLRGA VLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFP 
Sbjct: 61   HIGHAFSVSKLEFAAAYHRLRGARVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPS 120

Query: 121  EIEEQENLKAEAEDANESNPTLPDKFKGKKSKAASKSSGQMYQWEIMRSFGLSDSEISKF 180
            EIE+QENLK EAEDANESNPTLPDKFKGKKSKAASKS GQMYQWEIMRSFGLSDSEISKF
Sbjct: 121  EIEDQENLKVEAEDANESNPTLPDKFKGKKSKAASKSGGQMYQWEIMRSFGLSDSEISKF 180

Query: 181  QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD 240
            Q PYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTD+NPFFDSFV+WQMRKLKSMGKIVKD
Sbjct: 181  QXPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDINPFFDSFVRWQMRKLKSMGKIVKD 240

Query: 241  VRYTIYSPLDGQPCADHDRATGEGVQPQDYTLIKMEVVPPFPPKMGVLVGRKVYLAAATL 300
            VRYTIYSPLDGQPCADHDRA+GEGVQPQDYTLIKMEVVPPFPPK+GVL GRKVYLAAATL
Sbjct: 241  VRYTIYSPLDGQPCADHDRASGEGVQPQDYTLIKMEVVPPFPPKIGVLEGRKVYLAAATL 300

Query: 301  RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSKVPEKPTCLIQLTG 360
            RPETMYGQTNAWVLPEG YGAFEINDTDVFIITERAALNLAYQKFS+VPE+PTCLI+LTG
Sbjct: 301  RPETMYGQTNAWVLPEGNYGAFEINDTDVFIITERAALNLAYQKFSRVPERPTCLIRLTG 360

Query: 361  NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 420
            NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA
Sbjct: 361  NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 420

Query: 421  KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCLDLKIKSQNEKDKLAEAKRLTYLKGFTD 480
            KYGVKDEWVLP+DIVPIIDIPEFGDRAAEKVCLDLKIKSQNEK+KLAEAKRLTYL+GFTD
Sbjct: 421  KYGVKDEWVLPFDIVPIIDIPEFGDRAAEKVCLDLKIKSQNEKEKLAEAKRLTYLRGFTD 480

Query: 481  GTLIVGEFAGRKVQEAKPLIRSKLIETGQAIPYSEPEKRVISRSGDECIVALTDQWYITY 540
            GTL+VGEFAGRKVQEAKPLIRSKLIETGQAIPYSEPEKRV SRSGDECIVALTDQWYITY
Sbjct: 481  GTLVVGEFAGRKVQEAKPLIRSKLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITY 540

Query: 541  GESEWKKLAEECLANMQLFSDETRHGFEHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 600
            GESEWKKLAEECLANM LFSDETRHGFEHTL WLNQWACSRSFGLGTRIPWDEQFLVESL
Sbjct: 541  GESEWKKLAEECLANMDLFSDETRHGFEHTLGWLNQWACSRSFGLGTRIPWDEQFLVESL 600

Query: 601  SDSTIYMAYYTIAHLLHNGDLYGSGASAIKPEQMTDEVWDFVFCGAAEPKSTGISKSILN 660
            SDSTIYMAYYTIAH LHNGD+Y SGASAIKPEQMTDEVWDF+FCGAAEPKS+ IS+SIL 
Sbjct: 601  SDSTIYMAYYTIAHFLHNGDMYASGASAIKPEQMTDEVWDFIFCGAAEPKSSSISQSILK 660

Query: 661  KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPRHHWPRGFRCNGHIMLNSEKMS 720
            +MKQEFEYWYPFDLRVSGKDLIQNHLTF IYNHTAIMPRHHWPRGFRCNGHIMLNSEKMS
Sbjct: 661  QMKQEFEYWYPFDLRVSGKDLIQNHLTFSIYNHTAIMPRHHWPRGFRCNGHIMLNSEKMS 720

Query: 721  KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 780
            KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL
Sbjct: 721  KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 780

Query: 781  QADSASFLRTGPPSTYADRVFENEINIAVKMTEQNYKDYMFREALKTGFYDLQAARDEYR 840
            +ADS+SFLRTG PSTYADRVFENEINIAVKMTEQ+YKDYMFREALKTGFYDLQAARDEYR
Sbjct: 781  KADSSSFLRTGHPSTYADRVFENEINIAVKMTEQHYKDYMFREALKTGFYDLQAARDEYR 840

Query: 841  FSCGAAGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPLADSPDLT 900
            FSCGA GMNRDLVFRFMDVQTRLITPICPHYAE+VWR+MLKKEGFVV AGWP ADSPDLT
Sbjct: 841  FSCGAGGMNRDLVFRFMDVQTRLITPICPHYAEHVWRDMLKKEGFVVTAGWPSADSPDLT 900

Query: 901  LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTTVIEDKKLTGLIYVNEQFDGWKA 960
            LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKK APVT+VIEDKKLTGLIYVNEQFDGWKA
Sbjct: 901  LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKSAPVTSVIEDKKLTGLIYVNEQFDGWKA 960

Query: 961  ECLRILRSKFDSTKRTFAPDSEIMEALQKSSVGQAVDFRQTQKLCMPFLRFKKDEAAALG 1020
            ECLRILRSKFDSTKRTFAPDSEIMEALQKSSVGQA DFRQTQKLCMPFLRFKKDEAAALG
Sbjct: 961  ECLRILRSKFDSTKRTFAPDSEIMEALQKSSVGQAADFRQTQKLCMPFLRFKKDEAAALG 1020

Query: 1021 VHALDLKLPFGEMEVLKENLELIKRQIGLEEVEVLCASDTDALTKAGALASLLKQNPPSP 1080
            V ALDLKLPFGEMEVLKENLELI+RQIGLEEVEVLCASD+DAL KAGAL SLLKQNPPSP
Sbjct: 1021 VQALDLKLPFGEMEVLKENLELIRRQIGLEEVEVLCASDSDALAKAGALVSLLKQNPPSP 1080

Query: 1081 GNPTAIFLTS 1091
            GNPTAIFLTS
Sbjct: 1081 GNPTAIFLTS 1090

BLAST of Spg027529 vs. TAIR 10
Match: AT1G09620.1 (ATP binding;leucine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP binding;aminoacyl-tRNA ligases )

HSP 1 Score: 1691.4 bits (4379), Expect = 0.0e+00
Identity = 804/1090 (73.76%), Postives = 950/1090 (87.16%), Query Frame = 0

Query: 1    MASESGKSFARRDSLREIEAKVRVLWEENDVFRAEACETPPKVGEKFFGNFPFPYMNGFL 60
            MASES KS+ARRD L EIEA VR  WE+ DVFRAE+CE  PK GEKFF  FPFPYMNG+L
Sbjct: 1    MASES-KSYARRDRLLEIEATVRKWWEDEDVFRAESCENLPKPGEKFFSTFPFPYMNGYL 60

Query: 61   HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR 120
            H+GHAFSLSK++FA+AYHRLRGANVLLPFGFHCTGMPIKASADKL REI+QFG+PPVF  
Sbjct: 61   HIGHAFSLSKVDFASAYHRLRGANVLLPFGFHCTGMPIKASADKLRREIEQFGNPPVFTA 120

Query: 121  EIEEQENLKAEAEDANESNPTLPDKFKGKKSKAASKSSGQMYQWEIMRSFGLSDSEISKF 180
            E +  +  + + E ++     +P +FKGKKSK A+K+ GQ+YQWEIMRSFGL+DSEI+ F
Sbjct: 121  E-DTTKVPEVQEESSDTIALPIPGQFKGKKSKVAAKAGGQVYQWEIMRSFGLTDSEIANF 180

Query: 181  QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD 240
            + P  WL +FPPLA+EDL+A+GLGCDWRRSF+TTD+NPFFD+FV+WQMRKLKSMGKIVKD
Sbjct: 181  REPSEWLYYFPPLAVEDLRAYGLGCDWRRSFVTTDVNPFFDAFVRWQMRKLKSMGKIVKD 240

Query: 241  VRYTIYSPLDGQPCADHDRATGEGVQPQDYTLIKMEVVPPFPPKMGVLVGRKVYLAAATL 300
             RYTI+SPLDGQPCADHDRATGEGVQPQ+YTLIKMEVV PFP K+G L G++V+LAAATL
Sbjct: 241  RRYTIFSPLDGQPCADHDRATGEGVQPQEYTLIKMEVVKPFPLKLGPLEGKRVFLAAATL 300

Query: 301  RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSKVPEKPTCLIQLTG 360
            RPETMYGQTNAWVLP+GKYGA+EI++T+VFI+TERAALNLAYQ FSK P++P+CL++LTG
Sbjct: 301  RPETMYGQTNAWVLPDGKYGAYEISETEVFILTERAALNLAYQNFSKNPQEPSCLVELTG 360

Query: 361  NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 420
             DLIGLPL+SPL+ NEIIYALPMLTILT+KGTGIVTSVPSDAPDDYMA+ DL  KPAL+ 
Sbjct: 361  YDLIGLPLRSPLSVNEIIYALPMLTILTNKGTGIVTSVPSDAPDDYMALQDLIKKPALQD 420

Query: 421  KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCLDLKIKSQNEKDKLAEAKRLTYLKGFTD 480
            KYGVK EW LP +I+PII+IPEFGD+AAEKVCLDLKIKSQN+K+KLAEAKRLTYLKGFT+
Sbjct: 421  KYGVKTEW-LPTEIIPIINIPEFGDKAAEKVCLDLKIKSQNDKEKLAEAKRLTYLKGFTE 480

Query: 481  GTLIVGEFAGRKVQEAKPLIRSKLIETGQAIPYSEPEKRVISRSGDECIVALTDQWYITY 540
            GT+++GEF GRKVQE KP+I++KLIETG+AI YSEPEK V+SRSGDEC+VALTDQWYITY
Sbjct: 481  GTMLIGEFFGRKVQEIKPIIKTKLIETGEAIIYSEPEKPVMSRSGDECVVALTDQWYITY 540

Query: 541  GESEWKKLAEECLANMQLFSDETRHGFEHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 600
            GESEW+K+AEECL+ M L+SDETRHGFEHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL
Sbjct: 541  GESEWRKIAEECLSKMNLYSDETRHGFEHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 600

Query: 601  SDSTIYMAYYTIAHLLHNGDLYGSGASAIKPEQMTDEVWDFVFCGAAEPKSTGISKSILN 660
            SDS++YMAYYT+AH+ H+GD+Y    S I+P+QM DEVW+++FC    PKS+ I  ++L+
Sbjct: 601  SDSSLYMAYYTVAHIFHDGDMYKGSKSLIRPQQMNDEVWEYLFCDGPYPKSSDIPSAVLS 660

Query: 661  KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPRHHWPRGFRCNGHIMLNSEKMS 720
            +MKQEF+YWYP DLRVSGKDLIQNHLTF IYNHTA+M   +WPRG RCNGHIMLNSEKMS
Sbjct: 661  EMKQEFDYWYPLDLRVSGKDLIQNHLTFFIYNHTALMANRNWPRGIRCNGHIMLNSEKMS 720

Query: 721  KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 780
            KSTGNFRTLRQ+IEEFSA  TRF LADAGDGVDDANF FETANAAILRLTKE+ WME++L
Sbjct: 721  KSTGNFRTLRQSIEEFSATGTRFCLADAGDGVDDANFAFETANAAILRLTKELTWMEEVL 780

Query: 781  QADSASFLRTGPPSTYADRVFENEINIAVKMTEQNYKDYMFREALKTGFYDLQAARDEYR 840
              +S+  LRTGPPSTYAD+VFEN++NIA+++TE+ YKD +FREALK GFYDLQAARDEYR
Sbjct: 781  DVESS--LRTGPPSTYADKVFENDMNIALRLTERAYKDCLFREALKNGFYDLQAARDEYR 840

Query: 841  FSCGAAGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPLADSPDLT 900
             SCG  GM+ DL+ +FMDVQTRLI PICPH+A+YVWR++L KEG V+ AGWP ++ PDL 
Sbjct: 841  LSCGTGGMHHDLLLKFMDVQTRLIVPICPHFADYVWRKVLNKEGCVLTAGWPPSNEPDLV 900

Query: 901  LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTTVIEDKKLTGLIYVNEQFDGWKA 960
            LKSANKYLQDSIVLMRKLLQKQL GSKKG KKGA VT V E  KL GL+YVNEQFDGW+A
Sbjct: 901  LKSANKYLQDSIVLMRKLLQKQLSGSKKGAKKGAQVTAVPEG-KLKGLVYVNEQFDGWRA 960

Query: 961  ECLRILRSKFDSTKRTFAPDSEIMEALQKSSVGQAVDFRQTQKLCMPFLRFKKDEAAALG 1020
             CLRIL+S+FD    +F PD+E++  L  + + +  + +  QK+CMPFL+FKKDEA ++G
Sbjct: 961  HCLRILQSRFDQQTCSFPPDTEMLAELSATLLQEGKNLKAIQKVCMPFLKFKKDEAISIG 1020

Query: 1021 VHALDLKLPFGEMEVLKENLELIKRQIGLEEVEVLCASDTDALTKAGALASLLKQNPPSP 1080
              AL+L+LPFGE+EVL+ N +LI+RQ+GLEEVE+  ASD D ++ AG  ASLL QNPPSP
Sbjct: 1021 TQALNLRLPFGEIEVLQSNKDLIRRQLGLEEVEIYSASDPDDVSIAGPHASLLTQNPPSP 1080

Query: 1081 GNPTAIFLTS 1091
            G+PTAIF+TS
Sbjct: 1081 GSPTAIFVTS 1084

BLAST of Spg027529 vs. TAIR 10
Match: AT2G31170.1 (Cysteinyl-tRNA synthetase, class Ia family protein )

HSP 1 Score: 47.0 bits (110), Expect = 1.1e-04
Identity = 37/127 (29.13%), Postives = 58/127 (45.67%), Query Frame = 0

Query: 670 YPFDLRVSGKDLIQNHLTFCIYNHTAIMPRHHWPRGFRCNGHIMLNSEKMSKSTGNFRTL 729
           Y FD+   G DL+  H    I    A     +       NG + ++SEKMSKS GNF T+
Sbjct: 281 YSFDIHGGGMDLVFPHHENEIAQSCAACDSSNISYWIH-NGFVTVDSEKMSKSLGNFFTI 340

Query: 730 RQAIEEFSADATRFSLADA--GDGVDDANFVFETANAAILRLTKEIAWMEDILQADSASF 789
           RQ I+ +   A R  L        ++ ++F+ E+A+  I  + + +   E  L    ++F
Sbjct: 341 RQVIDLYHPLALRLFLMGTHYRSPINYSDFLLESASERIFYIYQTLHDCESALGEKDSTF 400

Query: 790 LRTGPPS 795
                PS
Sbjct: 401 ENGSVPS 406

BLAST of Spg027529 vs. TAIR 10
Match: AT2G31170.2 (Cysteinyl-tRNA synthetase, class Ia family protein )

HSP 1 Score: 47.0 bits (110), Expect = 1.1e-04
Identity = 37/127 (29.13%), Postives = 58/127 (45.67%), Query Frame = 0

Query: 670 YPFDLRVSGKDLIQNHLTFCIYNHTAIMPRHHWPRGFRCNGHIMLNSEKMSKSTGNFRTL 729
           Y FD+   G DL+  H    I    A     +       NG + ++SEKMSKS GNF T+
Sbjct: 119 YSFDIHGGGMDLVFPHHENEIAQSCAACDSSNISYWIH-NGFVTVDSEKMSKSLGNFFTI 178

Query: 730 RQAIEEFSADATRFSLADA--GDGVDDANFVFETANAAILRLTKEIAWMEDILQADSASF 789
           RQ I+ +   A R  L        ++ ++F+ E+A+  I  + + +   E  L    ++F
Sbjct: 179 RQVIDLYHPLALRLFLMGTHYRSPINYSDFLLESASERIFYIYQTLHDCESALGEKDSTF 238

Query: 790 LRTGPPS 795
                PS
Sbjct: 239 ENGSVPS 244

BLAST of Spg027529 vs. TAIR 10
Match: AT5G49030.1 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 45.4 bits (106), Expect = 3.3e-04
Identity = 29/91 (31.87%), Postives = 43/91 (47.25%), Query Frame = 0

Query: 9   FARRDSLREIEAKVRVLWEENDVFRAEACETPPKVGEKFFGNFPFPYMNGFLHLGHAFSL 68
           F  R +    E +++ LWEEN VF+  +       G  F  +   PY NG LH+GHA + 
Sbjct: 113 FGMRANSLTREPELQKLWEENQVFKRVSDNNN---GGSFILHDGPPYANGDLHMGHALNK 172

Query: 69  SKLEFAAAYHRLRGANVLLPFGFHCTGMPIK 100
              +    Y  L+   V    G+ C G+PI+
Sbjct: 173 ILKDIINRYKLLQNYKVQYVPGWDCHGLPIE 200

BLAST of Spg027529 vs. TAIR 10
Match: AT5G49030.2 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 45.4 bits (106), Expect = 3.3e-04
Identity = 29/91 (31.87%), Postives = 43/91 (47.25%), Query Frame = 0

Query: 9   FARRDSLREIEAKVRVLWEENDVFRAEACETPPKVGEKFFGNFPFPYMNGFLHLGHAFSL 68
           F  R +    E +++ LWEEN VF+  +       G  F  +   PY NG LH+GHA + 
Sbjct: 113 FGMRANSLTREPELQKLWEENQVFKRVSDNNN---GGSFILHDGPPYANGDLHMGHALNK 172

Query: 69  SKLEFAAAYHRLRGANVLLPFGFHCTGMPIK 100
              +    Y  L+   V    G+ C G+PI+
Sbjct: 173 ILKDIINRYKLLQNYKVQYVPGWDCHGLPIE 200

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022948270.10.0e+0097.06leucine--tRNA ligase, cytoplasmic-like [Cucurbita moschata][more]
KAG7036878.10.0e+0097.06Leucine--tRNA ligase, cytoplasmic [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6607189.10.0e+0097.06Leucine--tRNA ligase, cytoplasmic, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023523654.10.0e+0096.70leucine--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo][more]
XP_022998939.10.0e+0095.96leucine--tRNA ligase, cytoplasmic-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
F4I1160.0e+0073.76Leucine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At1g09620 P... [more]
Q5R6140.0e+0050.28Leucine--tRNA ligase, cytoplasmic OS=Pongo abelii OX=9601 GN=LARS1 PE=2 SV=1[more]
Q9P2J50.0e+0050.38Leucine--tRNA ligase, cytoplasmic OS=Homo sapiens OX=9606 GN=LARS1 PE=1 SV=2[more]
Q8BMJ25.4e-31049.17Leucine--tRNA ligase, cytoplasmic OS=Mus musculus OX=10090 GN=Lars1 PE=1 SV=2[more]
Q099963.4e-29648.78Leucine--tRNA ligase OS=Caenorhabditis elegans OX=6239 GN=lars-1 PE=3 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1G9C60.0e+0097.06Leucyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111451993 PE=3 SV=1[more]
A0A6J1KFQ20.0e+0095.96Leucyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111493448 PE=3 SV=1[more]
A0A6J1I0H80.0e+0095.14Leucyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111469399 PE=3 SV=1[more]
A0A6J1HLL70.0e+0094.95Leucyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111464710 PE=3 SV=1[more]
A0A6J1DSM30.0e+0094.95Leucyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111023537 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G09620.10.0e+0073.76ATP binding;leucine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP b... [more]
AT2G31170.11.1e-0429.13Cysteinyl-tRNA synthetase, class Ia family protein [more]
AT2G31170.21.1e-0429.13Cysteinyl-tRNA synthetase, class Ia family protein [more]
AT5G49030.13.3e-0431.87tRNA synthetase class I (I, L, M and V) family protein [more]
AT5G49030.23.3e-0431.87tRNA synthetase class I (I, L, M and V) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 1033..1053
NoneNo IPR availableGENE3D1.10.730.10coord: 764..879
e-value: 5.0E-6
score: 28.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 126..154
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 126..151
NoneNo IPR availablePANTHERPTHR45794:SF4BNAC08G42640D PROTEINcoord: 1..1090
NoneNo IPR availableCDDcd00812LeuRS_corecoord: 531..759
e-value: 1.53985E-72
score: 241.768
NoneNo IPR availableCDDcd07959Anticodon_Ia_Leu_AEccoord: 757..879
e-value: 3.83389E-43
score: 150.819
NoneNo IPR availableSUPERFAMILY52374Nucleotidylyl transferasecoord: 15..763
IPR009008Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domainGENE3D3.90.740.10coord: 266..523
e-value: 2.2E-244
score: 814.4
IPR009008Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domainSUPERFAMILY50677ValRS/IleRS/LeuRS editing domaincoord: 289..517
IPR002300Aminoacyl-tRNA synthetase, class IaPFAMPF00133tRNA-synt_1coord: 25..104
e-value: 6.2E-9
score: 34.7
coord: 196..756
e-value: 2.0E-29
score: 102.3
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 18..754
e-value: 2.2E-244
score: 814.4
IPR013155Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-bindingPFAMPF08264Anticodon_1coord: 798..921
e-value: 2.0E-12
score: 47.3
IPR004493Leucyl-tRNA synthetase, class Ia, archaeal/eukaryotic cytosolicTIGRFAMTIGR00395TIGR00395coord: 17..1053
e-value: 2.0E-272
score: 904.4
IPR004493Leucyl-tRNA synthetase, class Ia, archaeal/eukaryotic cytosolicPANTHERPTHR45794LEUCYL-TRNA SYNTHETASEcoord: 1..1090
IPR001412Aminoacyl-tRNA synthetase, class I, conserved sitePROSITEPS00178AA_TRNA_LIGASE_Icoord: 54..65
IPR009080Aminoacyl-tRNA synthetase, class Ia, anticodon-bindingSUPERFAMILY47323Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetasescoord: 790..932

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg027529.1Spg027529.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0106074 aminoacyl-tRNA metabolism involved in translational fidelity
biological_process GO:0006429 leucyl-tRNA aminoacylation
biological_process GO:0006418 tRNA aminoacylation for protein translation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0002161 aminoacyl-tRNA editing activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0004823 leucine-tRNA ligase activity
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding