Spg027417 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg027417 vs. NCBI nr
Match: XP_023546104.1 (UPF0481 protein At3g47200-like isoform X2 [Cucurbita pepo subsp. pepo] >XP_023546105.1 UPF0481 protein At3g47200-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 285.0 bits (728), Expect = 1.0e-72
Identity = 170/391 (43.48%), Postives = 233/391 (59.59%), Query Frame = 0

Query: 7   DEIIKVICKESENGVSPSCSKCSIYRVPEPLRSVNPKAYTPQLISIGPLHHANKDVKCFE 66
           D ++  I +  +  VS   S  +IY+VPEPLRS+ P+AYTP +ISIGPLH   KD+    
Sbjct: 53  DLVVLSINRILQQAVSSGSSDGTIYKVPEPLRSIKPEAYTPTVISIGPLHSGRKDLMANS 112

Query: 67  QKCRYFQHFLDLTGLRQEEIVNQVQTWEEEARMCYPEAEHVNMKSLDFVKLLVLDGCFVL 126
            K RY Q+FL+LT L    IV  V+TWE+ AR CY  AE + M   +FV+LLV DGCFV+
Sbjct: 113 LKPRYLQNFLNLTQLPTNTIVEIVKTWEKRARYCY--AESIEMSRDEFVELLVFDGCFVV 172

Query: 127 MHMID-SLISMRLFDQSFSWRSEDEIFKDMLLLENQLPFFILQNLYCFCSSS---LQDIS 186
           MH+I  S   +R  D S  W+  DE+F D++LLENQLPFF+LQ+LY  C+SS    + + 
Sbjct: 173 MHLISYSFFELRASDMSNLWKFWDELFCDLILLENQLPFFLLQSLYDLCASSQPFSKGVR 232

Query: 187 FLDLTREYFCNAYEDISY------LETEFPRDPNHFVDLVRMHLTFPSDELENTTHSLLC 246
           F++L  +YF  +++   +      L        NHFVDL+R+H T    +  +  H+   
Sbjct: 233 FIELVHQYFIESHKGGLFSLKEHVLLAGIGVQVNHFVDLLRLHFTHTRSDKMSFQHT--- 292

Query: 247 FPSWTATKLHSCSVSFKKRNKKSQSSGKKGMMDIEIKHRGGIIRMSEIIINDTFQISVRN 306
           F    AT+LH C V FK        +GK     I  K +GG +++ +I I D F+  VRN
Sbjct: 293 FWPPNATQLHECGVIFK--------TGK----GIAFKDQGGCLQLPQINIYDDFEKRVRN 352

Query: 307 VIAYEQCHQKYLMNNKARRFTMFMHSLIQTNSDVTLLIDLGIIHNNFGSIEQITKLFNNL 366
           +IAYEQCH    + N+   F +FM  L+QT+ DV LLI+ GIIHNNFGSI ++T+LFNNL
Sbjct: 353 LIAYEQCHFCSELRNEVSNFAVFMQCLVQTDQDVKLLIEGGIIHNNFGSINEVTQLFNNL 412

Query: 367 CEHVLIKREFNYLRSSEMEKYCKRRRLRWIN 388
            +H+             M+ YCKR R RWI+
Sbjct: 413 GKHICPGINSYNSDCKRMKDYCKRPRHRWIS 426

BLAST of Spg027417 vs. NCBI nr
Match: XP_023546101.1 (UPF0481 protein At3g47200-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023546102.1 UPF0481 protein At3g47200-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023546103.1 UPF0481 protein At3g47200-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 285.0 bits (728), Expect = 1.0e-72
Identity = 170/391 (43.48%), Postives = 233/391 (59.59%), Query Frame = 0

Query: 7   DEIIKVICKESENGVSPSCSKCSIYRVPEPLRSVNPKAYTPQLISIGPLHHANKDVKCFE 66
           D ++  I +  +  VS   S  +IY+VPEPLRS+ P+AYTP +ISIGPLH   KD+    
Sbjct: 152 DLVVLSINRILQQAVSSGSSDGTIYKVPEPLRSIKPEAYTPTVISIGPLHSGRKDLMANS 211

Query: 67  QKCRYFQHFLDLTGLRQEEIVNQVQTWEEEARMCYPEAEHVNMKSLDFVKLLVLDGCFVL 126
            K RY Q+FL+LT L    IV  V+TWE+ AR CY  AE + M   +FV+LLV DGCFV+
Sbjct: 212 LKPRYLQNFLNLTQLPTNTIVEIVKTWEKRARYCY--AESIEMSRDEFVELLVFDGCFVV 271

Query: 127 MHMID-SLISMRLFDQSFSWRSEDEIFKDMLLLENQLPFFILQNLYCFCSSS---LQDIS 186
           MH+I  S   +R  D S  W+  DE+F D++LLENQLPFF+LQ+LY  C+SS    + + 
Sbjct: 272 MHLISYSFFELRASDMSNLWKFWDELFCDLILLENQLPFFLLQSLYDLCASSQPFSKGVR 331

Query: 187 FLDLTREYFCNAYEDISY------LETEFPRDPNHFVDLVRMHLTFPSDELENTTHSLLC 246
           F++L  +YF  +++   +      L        NHFVDL+R+H T    +  +  H+   
Sbjct: 332 FIELVHQYFIESHKGGLFSLKEHVLLAGIGVQVNHFVDLLRLHFTHTRSDKMSFQHT--- 391

Query: 247 FPSWTATKLHSCSVSFKKRNKKSQSSGKKGMMDIEIKHRGGIIRMSEIIINDTFQISVRN 306
           F    AT+LH C V FK        +GK     I  K +GG +++ +I I D F+  VRN
Sbjct: 392 FWPPNATQLHECGVIFK--------TGK----GIAFKDQGGCLQLPQINIYDDFEKRVRN 451

Query: 307 VIAYEQCHQKYLMNNKARRFTMFMHSLIQTNSDVTLLIDLGIIHNNFGSIEQITKLFNNL 366
           +IAYEQCH    + N+   F +FM  L+QT+ DV LLI+ GIIHNNFGSI ++T+LFNNL
Sbjct: 452 LIAYEQCHFCSELRNEVSNFAVFMQCLVQTDQDVKLLIEGGIIHNNFGSINEVTQLFNNL 511

Query: 367 CEHVLIKREFNYLRSSEMEKYCKRRRLRWIN 388
            +H+             M+ YCKR R RWI+
Sbjct: 512 GKHICPGINSYNSDCKRMKDYCKRPRHRWIS 525

BLAST of Spg027417 vs. NCBI nr
Match: XP_022961897.1 (UPF0481 protein At3g47200-like isoform X3 [Cucurbita moschata] >XP_022961898.1 UPF0481 protein At3g47200-like isoform X3 [Cucurbita moschata] >XP_022961899.1 UPF0481 protein At3g47200-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 284.6 bits (727), Expect = 1.4e-72
Identity = 170/391 (43.48%), Postives = 231/391 (59.08%), Query Frame = 0

Query: 7   DEIIKVICKESENGVSPSCSKCSIYRVPEPLRSVNPKAYTPQLISIGPLHHANKDVKCFE 66
           D ++  I +  +  VS   S  +IY+VPEPLRS+ P+AYTP +ISIGPLH   KD+    
Sbjct: 53  DPVVLSINRILQQAVSSGSSDGTIYKVPEPLRSIKPEAYTPTVISIGPLHSGRKDLTANS 112

Query: 67  QKCRYFQHFLDLTGLRQEEIVNQVQTWEEEARMCYPEAEHVNMKSLDFVKLLVLDGCFVL 126
            K  Y Q+FL+LT L    IV  V+TWE+ AR CY  AE + M   +FV+LLV DGCFV+
Sbjct: 113 LKPMYLQNFLNLTKLPTNTIVETVKTWEKRARYCY--AESIEMNRDEFVELLVFDGCFVV 172

Query: 127 MHMID-SLISMRLFDQSFSWRSEDEIFKDMLLLENQLPFFILQNLYCFCSSS---LQDIS 186
           MH+I  S   +R  D S  W+   E+F D++LLENQLPFF+LQ+LY  C+SS   L+ + 
Sbjct: 173 MHLIGYSFFELRASDMSNLWKFWYELFCDLILLENQLPFFLLQSLYDLCASSQPLLKGVH 232

Query: 187 FLDLTREYFCNAYEDISY------LETEFPRDPNHFVDLVRMHLTFPSDELENTTHSLLC 246
           F++L  +YF  +++   +      L        NHFVDL+R+H T    +  +  H+   
Sbjct: 233 FIELVHQYFIESHKGGLFSLKEHVLLAGIGVQVNHFVDLLRLHFTHTRSDETSFQHT--- 292

Query: 247 FPSWTATKLHSCSVSFKKRNKKSQSSGKKGMMDIEIKHRGGIIRMSEIIINDTFQISVRN 306
           F    ATKLH C V FK         GK     I  K +GG +++ +I I D F+  VRN
Sbjct: 293 FWPPNATKLHECGVIFK--------MGK----GIAFKDQGGCLQLPQINIYDDFEKRVRN 352

Query: 307 VIAYEQCHQKYLMNNKARRFTMFMHSLIQTNSDVTLLIDLGIIHNNFGSIEQITKLFNNL 366
           +IAYEQCH    + N+   F +FM  L+QT+ DV LLI+ GIIHNNFGSI ++T+LFNNL
Sbjct: 353 LIAYEQCHIGSELRNEVSNFAVFMQCLVQTDQDVKLLIEGGIIHNNFGSINEVTQLFNNL 412

Query: 367 CEHVLIKREFNYLRSSEMEKYCKRRRLRWIN 388
            +H+             M+ YCKR R RWI+
Sbjct: 413 GKHICPGINSYNFDCKRMKDYCKRPRHRWIS 426

BLAST of Spg027417 vs. NCBI nr
Match: XP_022961893.1 (UPF0481 protein At3g47200-like isoform X1 [Cucurbita moschata] >XP_022961894.1 UPF0481 protein At3g47200-like isoform X1 [Cucurbita moschata] >XP_022961895.1 UPF0481 protein At3g47200-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 284.6 bits (727), Expect = 1.4e-72
Identity = 170/391 (43.48%), Postives = 231/391 (59.08%), Query Frame = 0

Query: 7   DEIIKVICKESENGVSPSCSKCSIYRVPEPLRSVNPKAYTPQLISIGPLHHANKDVKCFE 66
           D ++  I +  +  VS   S  +IY+VPEPLRS+ P+AYTP +ISIGPLH   KD+    
Sbjct: 152 DPVVLSINRILQQAVSSGSSDGTIYKVPEPLRSIKPEAYTPTVISIGPLHSGRKDLTANS 211

Query: 67  QKCRYFQHFLDLTGLRQEEIVNQVQTWEEEARMCYPEAEHVNMKSLDFVKLLVLDGCFVL 126
            K  Y Q+FL+LT L    IV  V+TWE+ AR CY  AE + M   +FV+LLV DGCFV+
Sbjct: 212 LKPMYLQNFLNLTKLPTNTIVETVKTWEKRARYCY--AESIEMNRDEFVELLVFDGCFVV 271

Query: 127 MHMID-SLISMRLFDQSFSWRSEDEIFKDMLLLENQLPFFILQNLYCFCSSS---LQDIS 186
           MH+I  S   +R  D S  W+   E+F D++LLENQLPFF+LQ+LY  C+SS   L+ + 
Sbjct: 272 MHLIGYSFFELRASDMSNLWKFWYELFCDLILLENQLPFFLLQSLYDLCASSQPLLKGVH 331

Query: 187 FLDLTREYFCNAYEDISY------LETEFPRDPNHFVDLVRMHLTFPSDELENTTHSLLC 246
           F++L  +YF  +++   +      L        NHFVDL+R+H T    +  +  H+   
Sbjct: 332 FIELVHQYFIESHKGGLFSLKEHVLLAGIGVQVNHFVDLLRLHFTHTRSDETSFQHT--- 391

Query: 247 FPSWTATKLHSCSVSFKKRNKKSQSSGKKGMMDIEIKHRGGIIRMSEIIINDTFQISVRN 306
           F    ATKLH C V FK         GK     I  K +GG +++ +I I D F+  VRN
Sbjct: 392 FWPPNATKLHECGVIFK--------MGK----GIAFKDQGGCLQLPQINIYDDFEKRVRN 451

Query: 307 VIAYEQCHQKYLMNNKARRFTMFMHSLIQTNSDVTLLIDLGIIHNNFGSIEQITKLFNNL 366
           +IAYEQCH    + N+   F +FM  L+QT+ DV LLI+ GIIHNNFGSI ++T+LFNNL
Sbjct: 452 LIAYEQCHIGSELRNEVSNFAVFMQCLVQTDQDVKLLIEGGIIHNNFGSINEVTQLFNNL 511

Query: 367 CEHVLIKREFNYLRSSEMEKYCKRRRLRWIN 388
            +H+             M+ YCKR R RWI+
Sbjct: 512 GKHICPGINSYNFDCKRMKDYCKRPRHRWIS 525

BLAST of Spg027417 vs. NCBI nr
Match: KAA0064930.1 (UPF0481 protein [Cucumis melo var. makuwa])

HSP 1 Score: 282.0 bits (720), Expect = 8.8e-72
Identity = 172/422 (40.76%), Postives = 248/422 (58.77%), Query Frame = 0

Query: 3   SEEEDEIIKVICKESENGVSPSCSK-----------CSIYRVPEPLRSVNPKAYTPQLIS 62
           ++  DE IK I       V+ S S+            SIY VP+ LR+ NPKAY+PQ+IS
Sbjct: 14  TKSRDEEIKEIYDRMVESVNHSISREISISTSFSRERSIYMVPKLLRNGNPKAYSPQVIS 73

Query: 63  IGPLHH--ANKDVKCFEQKCRYFQHFLDLTGLRQEEIVNQVQTWEEEARMCYPEAEHVNM 122
           IGPLH+     D+   E+K  Y  +FL +  L   E++N+   WEE AR  Y   E + M
Sbjct: 74  IGPLHYYRTQNDLTIKEKKGSYVLNFLTVAKLGWNEMINKFLCWEERARNYY--VETIKM 133

Query: 123 KSLDFVKLLVLDGCFVLMHMIDSLIS-MRLFDQSFSWRSEDEIFKDMLLLENQLPFFILQ 182
           +  +F++LL+ D CFV+M++I S+++  R  D SF WR  + IFKD+LLLENQLPFF+L 
Sbjct: 134 ERDEFIQLLIYDSCFVVMYIIGSMVAEFRDLDTSFLWRFSNGIFKDLLLLENQLPFFLLH 193

Query: 183 NLYCFCS---SSLQDISFLDLTREYFCNAYEDISYLETEF----PRDPNHFVDLVRMHLT 242
           +LY  C+    SL+DISF++L R YF    E +SY+   +      + NH VD +R+HLT
Sbjct: 194 HLYNLCAFAQPSLKDISFIELLRGYFTEVREGMSYVNEGYLDIDANEVNHLVDFLRIHLT 253

Query: 243 FPSDELENTTHSLLCF-PSW--TATKLHSCSVSFKKRNKKSQSSGKKGMMDIEIKHRGGI 302
            P         S+  F  SW  TAT+LH C +SF  +        K+ MM++  + R G+
Sbjct: 254 QPRHIPHFLDFSVDDFLSSWPLTATELHDCGISFHGQ--------KRCMMNVNFRERNGV 313

Query: 303 IRMSEIIINDTFQISVRNVIAYEQCHQKYLMNNKARRFTMFMHSLIQTNSDVTLLIDLGI 362
           ++M +III+D+F+I  RN+IAYE CH   L +     F MFMH LI TN DV+LL+D GI
Sbjct: 314 LKMPKIIIDDSFEILFRNMIAYEYCH---LKSKDVSNFGMFMHFLINTNKDVSLLVDDGI 373

Query: 363 IHNNFGSIEQITKLFNNLCEHVLIKREFNYLRSSEMEKYCKRRRLRWINETISTCHGPSY 401
           I N+ GS  +I  LFN+LC++++++R    +   +M++YCK RR RW+        G  +
Sbjct: 374 IQNHLGSTREIVVLFNDLCKNIIVERNLYSIECRKMKEYCKHRRHRWMTSLKRDYFGTPW 422

BLAST of Spg027417 vs. ExPASy Swiss-Prot
Match: Q9SD53 (UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 6.0e-23
Identity = 117/420 (27.86%), Postives = 194/420 (46.19%), Query Frame = 0

Query: 2   GSEEEDEIIKVICKESENGVSPSCSKCSIYRVPEPLRSVNPKAYTPQLISIGPLHHANKD 61
           GS+E   +++   KES          C I+RVPE   ++NPKAY P+++SIGP H+  K 
Sbjct: 30  GSKEPVLLLESAGKES----------CCIFRVPESFVALNPKAYKPKVVSIGPYHYGEKH 89

Query: 62  VKCFEQ-KCRYFQHFLDLTGLRQEE---IVNQVQTWEEEARMCYPEAEHVNMKSLDFVKL 121
           ++  +Q K R  Q FLD    +  E   +V  V   E++ R  Y E         D + +
Sbjct: 90  LQMIQQHKPRLLQLFLDEAKKKDVEENVLVKAVVDLEDKIRKSYSEELKTGH---DLMFM 149

Query: 122 LVLDGCFVLMHMIDSLISMRLFDQ---SFSWRSEDEIFKDMLLLENQLPFFILQNLY--- 181
           +VLDGCF+LM  +    ++ L +    S  W     I  D+LLLENQ+PFF+LQ LY   
Sbjct: 150 MVLDGCFILMVFLIMSGNIELSEDPIFSIPWLL-SSIQSDLLLLENQVPFFVLQTLYVGS 209

Query: 182 -CFCSSSLQDISFLDLTREYFCNAYE-DISYLETEFPRDPNHFVDLVR---MHLTFPSDE 241
               SS L  I+F      +F N  + + SY E        H +DL+R   +  T  SD+
Sbjct: 210 KIGVSSDLNRIAF-----HFFKNPIDKEGSYWEKHRNYKAKHLLDLIRETFLPNTSESDK 269

Query: 242 LEN----------------TTHSLLCFPSWTATKLHSCSVSFKKRNKKSQSSGKKGMMDI 301
             +                +  S       +A +L    + F+ R  K  S     ++++
Sbjct: 270 ASSPHVQVQLHEGKSGNVPSVDSKAVPLILSAKRLRLQGIKFRLRRSKEDS-----ILNV 329

Query: 302 EIKHRGGIIRMSEIIINDTFQISVRNVIAYEQCHQKYLMNNKARRFTMFMHSLIQTNSDV 361
            +K     +++ ++  +        N +A+EQ +     +N+   + +FM  L+    DV
Sbjct: 330 RLKKNK--LQIPQLRFDGFISSFFLNCVAFEQFYTD--SSNEITTYIVFMGCLLNNEEDV 389

Query: 362 TLL-IDLGIIHNNFGSIEQITKLFNNLCEHVLIKREFNYLRS--SEMEKYCKRRRLRWIN 388
           T L  D  II N+FGS  ++++ F  + + V+ + + +YL +    + +Y K    +W N
Sbjct: 390 TFLRNDKLIIENHFGSNNEVSEFFKTISKDVVFEVDTSYLNNVFKGVNEYTK----KWYN 417

BLAST of Spg027417 vs. ExPASy Swiss-Prot
Match: P0C897 (Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 PE=3 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 1.4e-08
Identity = 47/199 (23.62%), Postives = 90/199 (45.23%), Query Frame = 0

Query: 29  SIYRVPEPLRSVNPKAYTPQLISIGPLHHANKDVKCFEQKCRYFQHFL--DLTGLRQEEI 88
           SI+ VP+ L   +P +YTP  +SIGP H    ++   E+        +       R  ++
Sbjct: 44  SIFNVPKALMCSHPDSYTPHRVSIGPYHCLKPELHEMERYKLMIARKIRNQYNSFRFHDL 103

Query: 89  VNQVQTWEEEARMCYPEAEHVNMKSLDFVKLLVLDGCFVLMHMIDSLISMRLFDQSFSWR 148
           V ++Q+ E + R CY   +++       + ++ +D  F++  +   + S R  +   +  
Sbjct: 104 VEKLQSMEIKIRACY--HKYIGFNGETLLWIMAVDSSFLIEFL--KIYSFRKVETLINRV 163

Query: 149 SEDEIFKDMLLLENQLPFFILQNLYCFCSSSLQDISFLDLTREYFCNAYEDISYLETEFP 208
             +EI +D++++ENQ+P F+L+    F   S +     DL         +D+S L  +F 
Sbjct: 164 GHNEILRDIMMIENQIPLFVLRKTLEFQLESTESAD--DLLLSVLTGLCKDLSPLVIKFD 223

Query: 209 RDP---------NHFVDLV 217
            D          NH +D +
Sbjct: 224 DDQILKAQFQECNHILDFL 236

BLAST of Spg027417 vs. ExPASy TrEMBL
Match: A0A6J1HBC3 (UPF0481 protein At3g47200-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111462529 PE=4 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 6.6e-73
Identity = 170/391 (43.48%), Postives = 231/391 (59.08%), Query Frame = 0

Query: 7   DEIIKVICKESENGVSPSCSKCSIYRVPEPLRSVNPKAYTPQLISIGPLHHANKDVKCFE 66
           D ++  I +  +  VS   S  +IY+VPEPLRS+ P+AYTP +ISIGPLH   KD+    
Sbjct: 53  DPVVLSINRILQQAVSSGSSDGTIYKVPEPLRSIKPEAYTPTVISIGPLHSGRKDLTANS 112

Query: 67  QKCRYFQHFLDLTGLRQEEIVNQVQTWEEEARMCYPEAEHVNMKSLDFVKLLVLDGCFVL 126
            K  Y Q+FL+LT L    IV  V+TWE+ AR CY  AE + M   +FV+LLV DGCFV+
Sbjct: 113 LKPMYLQNFLNLTKLPTNTIVETVKTWEKRARYCY--AESIEMNRDEFVELLVFDGCFVV 172

Query: 127 MHMID-SLISMRLFDQSFSWRSEDEIFKDMLLLENQLPFFILQNLYCFCSSS---LQDIS 186
           MH+I  S   +R  D S  W+   E+F D++LLENQLPFF+LQ+LY  C+SS   L+ + 
Sbjct: 173 MHLIGYSFFELRASDMSNLWKFWYELFCDLILLENQLPFFLLQSLYDLCASSQPLLKGVH 232

Query: 187 FLDLTREYFCNAYEDISY------LETEFPRDPNHFVDLVRMHLTFPSDELENTTHSLLC 246
           F++L  +YF  +++   +      L        NHFVDL+R+H T    +  +  H+   
Sbjct: 233 FIELVHQYFIESHKGGLFSLKEHVLLAGIGVQVNHFVDLLRLHFTHTRSDETSFQHT--- 292

Query: 247 FPSWTATKLHSCSVSFKKRNKKSQSSGKKGMMDIEIKHRGGIIRMSEIIINDTFQISVRN 306
           F    ATKLH C V FK         GK     I  K +GG +++ +I I D F+  VRN
Sbjct: 293 FWPPNATKLHECGVIFK--------MGK----GIAFKDQGGCLQLPQINIYDDFEKRVRN 352

Query: 307 VIAYEQCHQKYLMNNKARRFTMFMHSLIQTNSDVTLLIDLGIIHNNFGSIEQITKLFNNL 366
           +IAYEQCH    + N+   F +FM  L+QT+ DV LLI+ GIIHNNFGSI ++T+LFNNL
Sbjct: 353 LIAYEQCHIGSELRNEVSNFAVFMQCLVQTDQDVKLLIEGGIIHNNFGSINEVTQLFNNL 412

Query: 367 CEHVLIKREFNYLRSSEMEKYCKRRRLRWIN 388
            +H+             M+ YCKR R RWI+
Sbjct: 413 GKHICPGINSYNFDCKRMKDYCKRPRHRWIS 426

BLAST of Spg027417 vs. ExPASy TrEMBL
Match: A0A6J1HD53 (UPF0481 protein At3g47200-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462529 PE=4 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 6.6e-73
Identity = 170/391 (43.48%), Postives = 231/391 (59.08%), Query Frame = 0

Query: 7   DEIIKVICKESENGVSPSCSKCSIYRVPEPLRSVNPKAYTPQLISIGPLHHANKDVKCFE 66
           D ++  I +  +  VS   S  +IY+VPEPLRS+ P+AYTP +ISIGPLH   KD+    
Sbjct: 152 DPVVLSINRILQQAVSSGSSDGTIYKVPEPLRSIKPEAYTPTVISIGPLHSGRKDLTANS 211

Query: 67  QKCRYFQHFLDLTGLRQEEIVNQVQTWEEEARMCYPEAEHVNMKSLDFVKLLVLDGCFVL 126
            K  Y Q+FL+LT L    IV  V+TWE+ AR CY  AE + M   +FV+LLV DGCFV+
Sbjct: 212 LKPMYLQNFLNLTKLPTNTIVETVKTWEKRARYCY--AESIEMNRDEFVELLVFDGCFVV 271

Query: 127 MHMID-SLISMRLFDQSFSWRSEDEIFKDMLLLENQLPFFILQNLYCFCSSS---LQDIS 186
           MH+I  S   +R  D S  W+   E+F D++LLENQLPFF+LQ+LY  C+SS   L+ + 
Sbjct: 272 MHLIGYSFFELRASDMSNLWKFWYELFCDLILLENQLPFFLLQSLYDLCASSQPLLKGVH 331

Query: 187 FLDLTREYFCNAYEDISY------LETEFPRDPNHFVDLVRMHLTFPSDELENTTHSLLC 246
           F++L  +YF  +++   +      L        NHFVDL+R+H T    +  +  H+   
Sbjct: 332 FIELVHQYFIESHKGGLFSLKEHVLLAGIGVQVNHFVDLLRLHFTHTRSDETSFQHT--- 391

Query: 247 FPSWTATKLHSCSVSFKKRNKKSQSSGKKGMMDIEIKHRGGIIRMSEIIINDTFQISVRN 306
           F    ATKLH C V FK         GK     I  K +GG +++ +I I D F+  VRN
Sbjct: 392 FWPPNATKLHECGVIFK--------MGK----GIAFKDQGGCLQLPQINIYDDFEKRVRN 451

Query: 307 VIAYEQCHQKYLMNNKARRFTMFMHSLIQTNSDVTLLIDLGIIHNNFGSIEQITKLFNNL 366
           +IAYEQCH    + N+   F +FM  L+QT+ DV LLI+ GIIHNNFGSI ++T+LFNNL
Sbjct: 452 LIAYEQCHIGSELRNEVSNFAVFMQCLVQTDQDVKLLIEGGIIHNNFGSINEVTQLFNNL 511

Query: 367 CEHVLIKREFNYLRSSEMEKYCKRRRLRWIN 388
            +H+             M+ YCKR R RWI+
Sbjct: 512 GKHICPGINSYNFDCKRMKDYCKRPRHRWIS 525

BLAST of Spg027417 vs. ExPASy TrEMBL
Match: A0A5A7VCL1 (UPF0481 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G002480 PE=4 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 4.3e-72
Identity = 172/422 (40.76%), Postives = 248/422 (58.77%), Query Frame = 0

Query: 3   SEEEDEIIKVICKESENGVSPSCSK-----------CSIYRVPEPLRSVNPKAYTPQLIS 62
           ++  DE IK I       V+ S S+            SIY VP+ LR+ NPKAY+PQ+IS
Sbjct: 14  TKSRDEEIKEIYDRMVESVNHSISREISISTSFSRERSIYMVPKLLRNGNPKAYSPQVIS 73

Query: 63  IGPLHH--ANKDVKCFEQKCRYFQHFLDLTGLRQEEIVNQVQTWEEEARMCYPEAEHVNM 122
           IGPLH+     D+   E+K  Y  +FL +  L   E++N+   WEE AR  Y   E + M
Sbjct: 74  IGPLHYYRTQNDLTIKEKKGSYVLNFLTVAKLGWNEMINKFLCWEERARNYY--VETIKM 133

Query: 123 KSLDFVKLLVLDGCFVLMHMIDSLIS-MRLFDQSFSWRSEDEIFKDMLLLENQLPFFILQ 182
           +  +F++LL+ D CFV+M++I S+++  R  D SF WR  + IFKD+LLLENQLPFF+L 
Sbjct: 134 ERDEFIQLLIYDSCFVVMYIIGSMVAEFRDLDTSFLWRFSNGIFKDLLLLENQLPFFLLH 193

Query: 183 NLYCFCS---SSLQDISFLDLTREYFCNAYEDISYLETEF----PRDPNHFVDLVRMHLT 242
           +LY  C+    SL+DISF++L R YF    E +SY+   +      + NH VD +R+HLT
Sbjct: 194 HLYNLCAFAQPSLKDISFIELLRGYFTEVREGMSYVNEGYLDIDANEVNHLVDFLRIHLT 253

Query: 243 FPSDELENTTHSLLCF-PSW--TATKLHSCSVSFKKRNKKSQSSGKKGMMDIEIKHRGGI 302
            P         S+  F  SW  TAT+LH C +SF  +        K+ MM++  + R G+
Sbjct: 254 QPRHIPHFLDFSVDDFLSSWPLTATELHDCGISFHGQ--------KRCMMNVNFRERNGV 313

Query: 303 IRMSEIIINDTFQISVRNVIAYEQCHQKYLMNNKARRFTMFMHSLIQTNSDVTLLIDLGI 362
           ++M +III+D+F+I  RN+IAYE CH   L +     F MFMH LI TN DV+LL+D GI
Sbjct: 314 LKMPKIIIDDSFEILFRNMIAYEYCH---LKSKDVSNFGMFMHFLINTNKDVSLLVDDGI 373

Query: 363 IHNNFGSIEQITKLFNNLCEHVLIKREFNYLRSSEMEKYCKRRRLRWINETISTCHGPSY 401
           I N+ GS  +I  LFN+LC++++++R    +   +M++YCK RR RW+        G  +
Sbjct: 374 IQNHLGSTREIVVLFNDLCKNIIVERNLYSIECRKMKEYCKHRRHRWMTSLKRDYFGTPW 422

BLAST of Spg027417 vs. ExPASy TrEMBL
Match: A0A1S3BD00 (UPF0481 protein At3g47200-like OS=Cucumis melo OX=3656 GN=LOC103488308 PE=4 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 4.3e-72
Identity = 172/422 (40.76%), Postives = 248/422 (58.77%), Query Frame = 0

Query: 3   SEEEDEIIKVICKESENGVSPSCSK-----------CSIYRVPEPLRSVNPKAYTPQLIS 62
           ++  DE IK I       V+ S S+            SIY VP+ LR+ NPKAY+PQ+IS
Sbjct: 14  TKSRDEEIKEIYDRMVESVNHSISREISISTSFSRERSIYMVPKLLRNGNPKAYSPQVIS 73

Query: 63  IGPLHH--ANKDVKCFEQKCRYFQHFLDLTGLRQEEIVNQVQTWEEEARMCYPEAEHVNM 122
           IGPLH+     D+   E+K  Y  +FL +  L   E++N+   WEE AR  Y   E + M
Sbjct: 74  IGPLHYYRTQNDLTIKEKKGSYVLNFLTVAKLGWNEMINKFLCWEERARNYY--VETIKM 133

Query: 123 KSLDFVKLLVLDGCFVLMHMIDSLIS-MRLFDQSFSWRSEDEIFKDMLLLENQLPFFILQ 182
           +  +F++LL+ D CFV+M++I S+++  R  D SF WR  + IFKD+LLLENQLPFF+L 
Sbjct: 134 ERDEFIQLLIYDSCFVVMYIIGSMVAEFRDLDTSFLWRFSNGIFKDLLLLENQLPFFLLH 193

Query: 183 NLYCFCS---SSLQDISFLDLTREYFCNAYEDISYLETEF----PRDPNHFVDLVRMHLT 242
           +LY  C+    SL+DISF++L R YF    E +SY+   +      + NH VD +R+HLT
Sbjct: 194 HLYNLCAFAQPSLKDISFIELLRGYFTEVREGMSYVNEGYLDIDANEVNHLVDFLRIHLT 253

Query: 243 FPSDELENTTHSLLCF-PSW--TATKLHSCSVSFKKRNKKSQSSGKKGMMDIEIKHRGGI 302
            P         S+  F  SW  TAT+LH C +SF  +        K+ MM++  + R G+
Sbjct: 254 QPRHIPHFLDFSVDDFLSSWPLTATELHDCGISFHGQ--------KRCMMNVNFRERNGV 313

Query: 303 IRMSEIIINDTFQISVRNVIAYEQCHQKYLMNNKARRFTMFMHSLIQTNSDVTLLIDLGI 362
           ++M +III+D+F+I  RN+IAYE CH   L +     F MFMH LI TN DV+LL+D GI
Sbjct: 314 LKMPKIIIDDSFEILFRNMIAYEYCH---LKSKDVSNFGMFMHFLINTNKDVSLLVDDGI 373

Query: 363 IHNNFGSIEQITKLFNNLCEHVLIKREFNYLRSSEMEKYCKRRRLRWINETISTCHGPSY 401
           I N+ GS  +I  LFN+LC++++++R    +   +M++YCK RR RW+        G  +
Sbjct: 374 IQNHLGSTREIVVLFNDLCKNIMVERNLYSIECRKMKEYCKHRRHRWMTSLKRDYFGTPW 422

BLAST of Spg027417 vs. ExPASy TrEMBL
Match: A0A0A0LPK8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G381700 PE=4 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 5.6e-72
Identity = 181/424 (42.69%), Postives = 251/424 (59.20%), Query Frame = 0

Query: 3   SEEEDEIIKVI------------CKESENGVSPSCSKCSIYRVPEPLRSVNPKAYTPQLI 62
           ++  DE IKVI             +E     S    + SIY VP+ LR  NPKAY+PQ+I
Sbjct: 14  TKSRDEEIKVIYDRMVGSVNQSMFREISRSASSFSKERSIYMVPKLLRKGNPKAYSPQVI 73

Query: 63  SIGPLHH---ANKDVKCFEQKCRYFQHFLDLTGLRQEEIVNQVQTWEEEARMCYPEAEHV 122
           SIGPLH+    N  +K  E+K  Y  +FL +  L   E++ +  +WEE AR  Y   E +
Sbjct: 74  SIGPLHYYRTQNDLIK--EKKGSYVLNFLTVAKLDWNEMIKKFLSWEERARNYY--VETI 133

Query: 123 NMKSLDFVKLLVLDGCFVLMHMIDSLIS-MRLFDQSFSWRSEDEIFKDMLLLENQLPFFI 182
            MK  +F++LL+ D CFV+M++I S+++  R  D SF WR  + IFKD+LLLENQLPFF+
Sbjct: 134 EMKRDEFIQLLIYDSCFVVMYVIGSMVAEFRDLDTSFLWRFSNGIFKDLLLLENQLPFFL 193

Query: 183 LQNLYCFCSS---SLQDISFLDLTREYFCNAYEDISYL-ETEFPRDP---NHFVDLVRMH 242
           L +LY  C+S   SL+DISF++L R YF    E +SY+ E  F  D    NH VD +R+H
Sbjct: 194 LNHLYNLCASAQPSLKDISFIELLRGYFSKVREGMSYVKEGYFDIDASAVNHLVDFLRIH 253

Query: 243 LTFPSDELENTTHSLLCF-PSW--TATKLHSCSVSFKKRNKKSQSSGKKGMMDIEIKHRG 302
           LT P         S+  F  SW  TAT+LH C +SF  +        KK MM++  K R 
Sbjct: 254 LTQPRHIPHFFGLSVDDFLSSWPLTATELHECGISFHGQ--------KKCMMNVSFKERR 313

Query: 303 GIIRMSEIIINDTFQISVRNVIAYEQCHQKYLMNNKARRFTMFMHSLIQTNSDVTLLIDL 362
           G+++M +III+D+F+I  RN+IAYE CH   L +  A  F MFMH LI TN DV+LL+D 
Sbjct: 314 GVLKMPKIIIDDSFEILFRNMIAYEYCH---LKSKDASNFGMFMHFLINTNEDVSLLVDD 373

Query: 363 GIIHNNFGSIEQITKLFNNLCEHVLIKREFNYLRSSEMEKYCKRRRLRWINETISTCHGP 401
           GII N  GS ++I  LF++LC++++I+R F  +    M++YCK RR RW+        G 
Sbjct: 374 GIIQNQLGSTKEIVVLFSDLCKNIMIERNFYSIACWRMKEYCKHRRHRWMTSLKRDYFGT 422

BLAST of Spg027417 vs. TAIR 10
Match: AT4G31980.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247, plant (InterPro:IPR004158), Protein of unknown function DUF862, eukaryotic (InterPro:IPR008580); BEST Arabidopsis thaliana protein match is: Plant protein of unknown function (DUF247) (TAIR:AT5G11290.1); Has 1967 Blast hits to 1844 proteins in 183 species: Archae - 0; Bacteria - 6; Metazoa - 223; Fungi - 83; Plants - 1477; Viruses - 0; Other Eukaryotes - 178 (source: NCBI BLink). )

HSP 1 Score: 174.5 bits (441), Expect = 1.8e-43
Identity = 126/396 (31.82%), Postives = 206/396 (52.02%), Query Frame = 0

Query: 1   MGSEEEDEIIKVICKESENGVSPSCSKCSIYRVPEPLRSVNPKAYTPQLISIGPLHHANK 60
           M   E D ++  I K     +S   +KC IY+VP  LR +NP AYTP+L+S GPLH   +
Sbjct: 267 MNQNEGDALVDSI-KAKLAFLSSLSTKCCIYKVPNKLRRLNPDAYTPRLVSFGPLHRGKE 326

Query: 61  DVKCFE-QKCRYFQHFLDLTGLRQEEIVNQVQTWEEEARMCYPEAEHVNMKSLDFVKLLV 120
           +++  E QK RY   F+  T    E++V   +TWE+ AR CY  AE V + S +FV++LV
Sbjct: 327 ELQAMEDQKYRYLLSFIPRTNSSLEDLVRLARTWEQNARSCY--AEDVKLHSDEFVEMLV 386

Query: 121 LDGCFVLMHMIDSLI------SMRLFDQSFSWRSEDEIFKDMLLLENQLPFFILQNLYCF 180
           +DG F++  ++ S        + R+F  S       ++ +DM+L+ENQLPFF+++ ++  
Sbjct: 387 VDGSFLVELLLRSHYPRLRGENDRIFGNSM---MITDVCRDMILIENQLPFFVVKEIFLL 446

Query: 181 CSSSLQD--ISFLDLTREYFCNAYEDISYLETEFPRDPNHFVDLVR-MHLTFPSDELENT 240
             +  Q    S + L + +F      I   + +F  +P HFVDL+R  +L     +LE T
Sbjct: 447 LLNYYQQGTPSIIQLAQRHFSYFLSRID--DEKFITEPEHFVDLLRSCYLPQFPIKLEYT 506

Query: 241 THSLLCFPSWTATKLHSCSVSFKKRNKKSQSSGKKGMMDIEIKHRGGIIRMSEIIINDTF 300
           T  +   P   AT+LH+  V FK     S          ++I    G++++  I+++D  
Sbjct: 507 TVKVDNAPE--ATELHTAGVRFKPAETSS--------CLLDISFADGVLKIPTIVVDDLT 566

Query: 301 QISVRNVIAYEQCHQKYLMNNKARRFTMFMHSLIQTNSDVTLLIDLGIIHNNFGSIEQIT 360
           +   +N+I +EQC      N     + M +   I++ +D  LLI  GII N  G+   ++
Sbjct: 567 ESLYKNIIGFEQCR---CSNKNFLDYIMLLGCFIKSPTDADLLIHSGIIVNYLGNSVDVS 626

Query: 361 KLFNNLCEHVLIKREFNY-LRSSEMEKYCKRRRLRW 386
            LFN++ + V+  R F + + S  ++ YC     RW
Sbjct: 627 NLFNSISKEVIYDRRFYFSMLSENLQAYCNTPWNRW 641

BLAST of Spg027417 vs. TAIR 10
Match: AT3G47210.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 133.3 bits (334), Expect = 4.7e-31
Identity = 114/375 (30.40%), Postives = 178/375 (47.47%), Query Frame = 0

Query: 28  CSIYRVPEPLRSVNPKAYTPQLISIGPLHHANKDVKCFEQ-KCRYFQHFLDLTGLRQEEI 87
           C I+RVP+    +NP+AY P+++SIGP HH  K ++  +Q K R+   FL    + ++ +
Sbjct: 93  CCIFRVPKSFAEMNPEAYKPKVVSIGPYHHGRKHLEMIQQHKLRFLHLFLRTASVDRDVL 152

Query: 88  VNQVQTWEEEARMCYPEAEHVNMKSLDFVKLLVLDGCFVLMHMIDSLISMRLFDQSFSWR 147
            N V  WE+E R  Y  +E +     + V +++LDGCF+LM ++     + L++      
Sbjct: 153 FNAVVDWEDEIRKSY--SEGLEGSPHELVYMMILDGCFILMLLLIVSRKIELYE------ 212

Query: 148 SEDEIF----------KDMLLLENQLPFFILQNLYCFCSSSLQDISFLDLTREYFCNAYE 207
           SED I            D+LLLENQ+PFF+LQ L+      +      DL R  F     
Sbjct: 213 SEDPILTIPWILPSIQSDLLLLENQVPFFVLQTLFDKSEIGVPG----DLNRMAFSFFNL 272

Query: 208 DISYLETEFPRDPN----HFVDLVRMHLTFPSDELEN-----------TTHSLLCFPSWT 267
            +   E  + +  N    H +DL+RM    P D  E+           ++  L    S  
Sbjct: 273 SMDKPERYWVKHRNFNAKHLLDLIRMSF-LPMDGYEDFQLTKGKSRKKSSSGLTLLLS-- 332

Query: 268 ATKLHSCSVSFKKRNKKSQSSGKKGMMDIEIKHRGGIIRMSEIIINDTFQISV-RNVIAY 327
           AT+L    + F  R      SG   M+DI +K     I +  +   D F IS+  N +A+
Sbjct: 333 ATRLSLQGIDFSLR------SGADSMLDIRLKKNRLQIPVLRL---DGFIISILLNCVAF 392

Query: 328 EQCHQKYLMNNKARRFTMFMHSLIQTNSDVTLLIDLGIIHNNFGSIEQITKLFNNLCEHV 376
           EQ + K    N    + +FM  L+    D T L    II N FGS ++++K F  +C+ V
Sbjct: 393 EQFYAK--STNHITSYVVFMGCLLNGKEDATFLSRRRIIENYFGSEKEVSKFFKTICKDV 441

BLAST of Spg027417 vs. TAIR 10
Match: AT2G36430.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 121.3 bits (303), Expect = 1.9e-27
Identity = 111/383 (28.98%), Postives = 174/383 (45.43%), Query Frame = 0

Query: 15  KESENGVSPSCSK--CSIYRVPEPLRSVNPKAYTPQLISIGPLHHANKDVKCFEQ-KCRY 74
           KE    +S +  K  CSI+RVP+ +   N + Y P+++SIGP H     +K  E+ K RY
Sbjct: 30  KEPPRLLSSAAGKPTCSIFRVPQSMIDCNGRCYEPRVVSIGPYHRGQTQLKMIEEHKWRY 89

Query: 75  FQHFLDLT-GLRQEEIVNQVQTWEEEARMCYPEAEHVNMKSLDFVKLLVLDGCFV--LMH 134
               L  T  L  E+ +  V+  EE AR CY E  H  M S +F +++VLDGCF+  L  
Sbjct: 90  LNVLLTRTQNLTLEDYMKSVKNVEEVARECYSETIH--MDSEEFNEMMVLDGCFLLELFR 149

Query: 135 MIDSLISMRLFDQ--SFSWRSEDEIFKDMLLLENQLPFFILQNLYCF--------CSSSL 194
            +++L+     D   + +W      ++D L LENQ+PFF+L+ L+           ++SL
Sbjct: 150 KVNNLVPFEPNDPLVAMAW-VLPFFYRDFLCLENQIPFFVLETLFNLTRGDNENETNASL 209

Query: 195 QDISFLDLTREYFCNAYEDISYLETEFPRDPNHFVDLVRMHLTFPSDELENTTHSLLCFP 254
           Q ++F            ED++  +        H +DL+R     P  EL     +     
Sbjct: 210 QSLAFA-FFNNMMHRTEEDLARFK---ELRAKHLLDLLRSSF-IPESELHTPPATNPGKE 269

Query: 255 SWTATKLHSCSVSFKKRNKKSQSSGKKGMMDIEIKHRGGIIRMSEIIINDTFQISVRNVI 314
              +  +HS S   +   K  +    +  + +  +H  G I M  I ++D     + N +
Sbjct: 270 KMPSHIIHSISKLRRAGIKLRELKDAESFLVVRFRH--GTIEMPAITVDDFMSSFLENCV 329

Query: 315 AYEQCHQKYLMNNKARRFTMFMHSLIQTNSDVTLLIDLGIIHNNFGSIEQITKLFNNLCE 374
           AYEQCH    M+     +   +  L  T  DV  L D  II N FG+  ++ K  N+L  
Sbjct: 330 AYEQCHVACSMH--FTTYATLLDCLTNTYKDVEYLCDQNIIENYFGTDTELAKFVNSLGR 389

Query: 375 HVLIKREFNYLRS--SEMEKYCK 380
            V       YL+    E+ +Y K
Sbjct: 390 DVAFDITQCYLKDLFEEVNEYYK 400

BLAST of Spg027417 vs. TAIR 10
Match: AT3G50180.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 117.5 bits (293), Expect = 2.7e-26
Identity = 109/389 (28.02%), Postives = 178/389 (45.76%), Query Frame = 0

Query: 24  SCSKCSIYRVPEPLRSVNPKAYTPQLISIGPLHHANKDVKCFE-QKCRYFQHFLDLTGLR 83
           S  K  IY+VP  L   + K+Y PQ +S+GP HH  +  +  E  K R     L  T   
Sbjct: 174 SWGKLCIYKVPHYLHGNDKKSYFPQTVSLGPYHHGRQQTQSMECHKWRAVNMVLKRTNQG 233

Query: 84  QEEIVNQVQTWEEEARMCYPEAEHVNMKSLDFVKLLVLDGCFV--LMHMIDSLISMRLFD 143
            E  ++ +   EE+AR CY  +  + + S +F ++L+LDGCF+  L+  ++       +D
Sbjct: 234 IEVFLDAMIELEEKARACYEGS--IVLSSNEFTEMLLLDGCFILELLQGVNEGFLKLGYD 293

Query: 144 QS---FSWR-SEDEIFKDMLLLENQLPFFILQNLYCFCSSSLQDISFLDLTREYFCNAYE 203
            +   F+ R S   I +DM++LENQLP F+L  L      +      ++L   +F     
Sbjct: 294 HNDPVFAVRGSMHSIQRDMIMLENQLPLFVLNRLLELQPGTQNQTGLVELVVRFFIPLMP 353

Query: 204 DISYL-ETEFPRDPN----HFVDLVRMHLTFPSDELENT------THSLLCFPSWTATKL 263
               L E   PR  +    H +D+    L FP    +         H     P  T T+L
Sbjct: 354 TAETLTENSPPRGVSNGELHCLDVFHRSLLFPRSSGKANYSRVADKHLQRVIP--TVTEL 413

Query: 264 HSCSVSFKKRNKKSQSSGKKGMMDIEIKHRGGIIRMSEIIINDTFQISVRNVIAYEQCHQ 323
                 F K NK  +          +IK   G + +  ++I+D  +    N+IA+EQCH 
Sbjct: 414 RDAGFKF-KLNKTDRF--------WDIKFSNGYLEIPGLLIHDGTKSLFLNLIAFEQCHI 473

Query: 324 KYLMNNKARRFTMFMHSLIQTNSDVTLLIDLGIIHNNFGSIEQITKLFNNLCEHVLIKRE 383
           +   +N    + +FM +LI +  D++ L   GII ++ GS  ++  +FN LC+ V+   +
Sbjct: 474 E--SSNDITSYIIFMDNLIDSPEDISYLHHCGIIEHSLGSNSEVADMFNQLCQEVVFDTK 533

Query: 384 FNYLRS--SEMEKYCKRRRLRWINETIST 393
             YL     E+ +  K+   R +N   +T
Sbjct: 534 DIYLSQLLIEVHRCYKQNYSRKLNSLKTT 547

BLAST of Spg027417 vs. TAIR 10
Match: AT5G22540.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 116.3 bits (290), Expect = 6.0e-26
Identity = 98/365 (26.85%), Postives = 174/365 (47.67%), Query Frame = 0

Query: 28  CSIYRVPEPLRSVNPKAYTPQLISIGPLHHANKDVKCFEQKCRYFQHF----LDLTGLRQ 87
           C I R+P+ L  +N KAY P+++SIGP HH  + +K  +Q  R F  F    ++  G   
Sbjct: 31  CCIVRIPQSLARINLKAYEPKIVSIGPYHHGKEHLKMTQQHKRRFLKFFVAKMEEKGFVP 90

Query: 88  EEIVNQVQTWEEEARMCYPEAEHVNMKSLDFVKLLVLDGCFV--LMHMIDSLISMRLFDQ 147
           +E+V  V + E   R  Y  +E + + S + V+++VLDGCF+  L  ++   +     D 
Sbjct: 91  QELVKAVSSLEGVIRGSY--SEDLGLDSENLVQMMVLDGCFILTLFFVVSGKVEYTNLDD 150

Query: 148 ---SFSWRSEDEIFKDMLLLENQLPFFILQNLY----CFCSSSLQDISFLDLTREYF-CN 207
                 W     I  D+LLLENQ+P+ +LQ L+        S L +I+F     E+F  +
Sbjct: 151 PIFRMPW-ILPSIRADLLLLENQVPYVLLQTLFETSKLVTCSGLNEIAF-----EFFNYS 210

Query: 208 AYEDISYLETEFPRDPNHFVDLVR---------MHLTFPSDELENTTHSLLCFPSWTATK 267
             +  ++ E  +  +  H +DL+R           +   S +     H  L F   +A K
Sbjct: 211 LQKPETFWEKHYGLEAKHLLDLIRKTFVPVPSQRRIKDHSSKSSFNDHEYLGF-VLSAKK 270

Query: 268 LHSCSVSFKKRNKKSQSSGKKGMMDIEIKHRGGIIRMSEIIINDTFQISVRNVIAYEQCH 327
           LH   + FK R K + S        ++I +  G++ +  ++++D       N +A+EQ +
Sbjct: 271 LHLRGIKFKPR-KNTDSI-------LDISYSNGVLHIPPVVMDDFTASIFLNCVAFEQLY 330

Query: 328 QKYLMNNKARRFTMFMHSLIQTNSDVTLLIDLGIIHNNFGSIEQITKLFNNLCEHVLIKR 370
                +N    +  FM  LI   SD + L +  I+ N FG+ +++++ +  + + + +  
Sbjct: 331 AD--SSNHITSYVAFMACLINEESDASFLSERRILENYFGTEDEVSRFYKRIGKDIALDL 376