Spg027392 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg027392
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptioncyclic nucleotide-gated ion channel 1-like
Locationscaffold7: 43897904 .. 43900788 (-)
RNA-Seq ExpressionSpg027392
SyntenySpg027392
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCAGAGGACTATGATCATGTGCAACAAAGTGAGAAGAAAGTTCCATGGTTGGTGGAACAATATTGGTTGCAATTGGCTTAAGAGTTTGGGGAAAATGGTAGGACGAGACTCGACATTGTTTAGAATTATTGTTGGTCGAAAACGAAAACTCCATTCTAAGAATGGAAGACTTGCGATGTTGTCTGTCATTGCAACCTCAGTGGATCCTTTATTCTTTTACATACTGTTTGTTAATGAAGATAGAAGGTGCATTGGATTCCACGACGAGTGGAAGACAGCAGCGGTAATTCTTCGCTCAGTCATTGACTTTCTCTACGCAATTCTTACTGCCTATTATTTTCATGATGGTTATCCGTTACTTAACGATGAAACTGACGGCGTTCTCGTGAGAGCTCGTAAATTCCTTCTGTCCTCCTCTCGTGTTGATCTTCTTGCATTTCTTCCCCTCCCTCAGGTATGCAAACTTCACTCTAATCCTTTCCAAGATTTTAAATGCCCAAAATTCCGAATACTTTTGTGCACGTGCAGTTGCTGGCTTTATTAGTCATTCCAAGATCCAAAGGACTCCATTTTATACATGCAATAAGGGCACTGAATTATGTTGTCCTTGTTCAATATTTGTCAAGGGTCATTCGAATCTACTCTTTCCTAATGAAATCTGGATGGAGCACCATTCTCCCACAAGCTGCAGAGGCTAGAGCTACATTCAATCTCATCCTCTATATGCTTGCAACTCATGTAACGTAACACTATTTAATTCATTCCTCCTATGATTTTCTGTTTTGGGCTTTTATTTTGGATCTATTATGTTAGGGTTAAGAGTGGTGTCATATATAAACCCTCTTAACCTATTTTGTCCTAATCTCTGTAATCTGCATACGTTTTACTCTTGTTTTCTACCTCTGTCCGTGGACGTAGCTAACACAACCACGTAAATCTGTGTGTTGTTTTCTTCGCCTTTTCATTCTTATTTGTTGGTTTACGATTTCTGTCATAACATCATCATTTATATATGTTAATGTTTTAGGTTGTTGGAGCATTTTGGTACCTGTCTACCATAGAGCGAAAAACAATGTGTTGGCATGGACGCTGCCATTATTGTCCTTTGAACTGTAATTACGTTCTTATTGCAAATAATGCCGACGATTTTTGTTCTGAACAAGCGGAAAATGATTCAAAAACTTATGACTTTGGGGTATTCAAAGATGCCCTTCCTATTTTGAATTCAAGAGATTTCATTTGGAAAATCTCTTATTGCTTTTGGTGGAGTCTGCAGAAATTGAGGTTTTTTTTTAGTCATCTCGTTGTCTCTGATAAACTTATACTCTTGCTATGTGATAATAGTTTTTCTTATGTGCAGCTCTTTTGGTCAAGACCTGAAAACCAGTGGACATTTGTTAGAAATCTACTTTGCAATCACCATTACCATTTCTGGCTTGGTATTATTTGCACTTCTTATAGGAAATTTGCAGGTCAGTAATTGAGCATATTTCAAAGCAAACAATAATATGTATTTAATCTTGATATGATGAGCTTATTTTTATATGTAACAGACATATTTGCAGTCAACCATTAGAAGCAAGGAGAAGATTAGATTGAAGGAGCAAGATATTGAGAACTGCATGACTAACTATTCTATCCCAGATAAGTTGAAGACGCAAGTTAGAAAATGTAAATTGTACAAGCAGCGAGAAGCCAAAGGTGTCGGCGTTGAGCAGCTTCTCCAAAATCTTCCCAACCATATAAGTAGGGATATAAAAGAACACCTTTGCTCTACTTCACTTTTCAATGTGAGTTCACTTTTCTATTCCTTTGGCCACCTTTAGTTCATTTTCATCCATATTACTTTTAAATTAATGTCCATTTTAGTCCATGTACTATGGGCTTTGGTTCATTTTGGTCTCTGTACTTTCAAAATGTTCATTTTGGTCTCGTTACATTTATATTTAGGTCTAAATACTATCTTGGTCCCTCTTCTTTCAACCTTAGTTTATTTTCGTTTTAGTACTTTCGACTTTTGTTCATTAAGTTCCCACACTTTCGAAACATCCCTTCTTTTGTCATTGTTTGAAAAAAATTAACACAAATTCTATACCCGTCATACGTATTTCAATATAAATTTAGAGGTCCATTTTAGGAAATTTATTGTAATGTCATGCTAAAATTATGATAAAAAATTAAATCAAATAGAAAATGAAATAACCAATAGAGTCATTTTTTTTAAAAGTATAGGGACCAAAACAAACATTACAAAAGTATAGAGACTTAAATAGACCATAATTTGAGAGTACATCAACCACATAAAAAGACCGAAATAAACAAAAACTAAAAAAGTAGAGGTACCAAACTAGTATTTAAACCTTCAATTTTTATGATACTATATATGTAGGTGCTCTTAAAGCTTGATAACATGGACAAGATATTGTCGGATGCAATCTATGATTTGCTAAAGCCAAAGCGATATATTGAACAAACGTTCATTGTTCAAGAAGGAGGACCAGTAGATGAAGTGGTCTTCATAATTCAAGGCAAGGTATTGGTCAGTTCCAAAAAAGATAGCAAGCCAGAAGAGTTGAACCGGCTTACCAAAGGTCACTTTTATGGAGAGGAACTTGTAGATTAGGCAAAAAATTCTTCAAAATTTCACACTCTCCCCATATCTACCAAAACCATTTATGCGCTCACCGACGTAGAAGTGTTTGTTCTTGTGGCAAAAGATTTAGAGTCTATTTGGAATCGTTGTTTAAGTGCTTAAAAACTGTTTTAAAGCACTTGAAAAGTCATTCCAAACCGACTTTAAACGACCAGAGACAAATTGTTTCGAGAAAAACATATATTTCTATATTTTAGTTCTATATTAAACGTAATGTGTGGCATTTTTCAA

mRNA sequence

ATGATCATGTGCAACAAAGTGAGAAGAAAGTTCCATGGTTGGTGGAACAATATTGGTTGCAATTGGCTTAAGAGTTTGGGGAAAATGGTAGGACGAGACTCGACATTGTTTAGAATTATTGTTGGTCGAAAACGAAAACTCCATTCTAAGAATGGAAGACTTGCGATGTTGTCTGTCATTGCAACCTCAGTGGATCCTTTATTCTTTTACATACTGTTTGTTAATGAAGATAGAAGGTGCATTGGATTCCACGACGAGTGGAAGACAGCAGCGGTAATTCTTCGCTCAGTCATTGACTTTCTCTACGCAATTCTTACTGCCTATTATTTTCATGATGGTTATCCGTTACTTAACGATGAAACTGACGGCGTTCTCGTGAGAGCTCGTAAATTCCTTCTGTCCTCCTCTCGTGTTGATCTTCTTGCATTTCTTCCCCTCCCTCAGTTGCTGGCTTTATTAGTCATTCCAAGATCCAAAGGACTCCATTTTATACATGCAATAAGGGCACTGAATTATGTTGTCCTTGTTCAATATTTGTCAAGGGTCATTCGAATCTACTCTTTCCTAATGAAATCTGGATGGAGCACCATTCTCCCACAAGCTGCAGAGGCTAGAGCTACATTCAATCTCATCCTCTATATGCTTGCAACTCATGTTGTTGGAGCATTTTGGTACCTGTCTACCATAGAGCGAAAAACAATGTGTTGGCATGGACGCTGCCATTATTGTCCTTTGAACTGTAATTACGTTCTTATTGCAAATAATGCCGACGATTTTTGTTCTGAACAAGCGGAAAATGATTCAAAAACTTATGACTTTGGGGTATTCAAAGATGCCCTTCCTATTTTGAATTCAAGAGATTTCATTTGGAAAATCTCTTATTGCTTTTGGTGGAGTCTGCAGAAATTGAGCTCTTTTGGTCAAGACCTGAAAACCAGTGGACATTTGTTAGAAATCTACTTTGCAATCACCATTACCATTTCTGGCTTGGTATTATTTGCACTTCTTATAGGAAATTTGCAGACATATTTGCAGTCAACCATTAGAAGCAAGGAGAAGATTAGATTGAAGGAGCAAGATATTGAGAACTGCATGACTAACTATTCTATCCCAGATAAGTTGAAGACGCAAGTTAGAAAATGTAAATTGTACAAGCAGCGAGAAGCCAAAGGTGTCGGCGTTGAGCAGCTTCTCCAAAATCTTCCCAACCATATAAGTAGGGATATAAAAGAACACCTTTGCTCTACTTCACTTTTCAATGTGCTCTTAAAGCTTGATAACATGGACAAGATATTGTCGGATGCAATCTATGATTTGCTAAAGCCAAAGCGATATATTGAACAAACGTTCATTGTTCAAGAAGGAGGACCAGTAGATGAAGTGGTCTTCATAATTCAAGGCAAGGTATTGGTCAGTTCCAAAAAAGATAGCAAGCCAGAAGAGTTGAACCGGCTTACCAAAGGTCACTTTTATGGAGAGGAACTTGTAGATTAG

Coding sequence (CDS)

ATGATCATGTGCAACAAAGTGAGAAGAAAGTTCCATGGTTGGTGGAACAATATTGGTTGCAATTGGCTTAAGAGTTTGGGGAAAATGGTAGGACGAGACTCGACATTGTTTAGAATTATTGTTGGTCGAAAACGAAAACTCCATTCTAAGAATGGAAGACTTGCGATGTTGTCTGTCATTGCAACCTCAGTGGATCCTTTATTCTTTTACATACTGTTTGTTAATGAAGATAGAAGGTGCATTGGATTCCACGACGAGTGGAAGACAGCAGCGGTAATTCTTCGCTCAGTCATTGACTTTCTCTACGCAATTCTTACTGCCTATTATTTTCATGATGGTTATCCGTTACTTAACGATGAAACTGACGGCGTTCTCGTGAGAGCTCGTAAATTCCTTCTGTCCTCCTCTCGTGTTGATCTTCTTGCATTTCTTCCCCTCCCTCAGTTGCTGGCTTTATTAGTCATTCCAAGATCCAAAGGACTCCATTTTATACATGCAATAAGGGCACTGAATTATGTTGTCCTTGTTCAATATTTGTCAAGGGTCATTCGAATCTACTCTTTCCTAATGAAATCTGGATGGAGCACCATTCTCCCACAAGCTGCAGAGGCTAGAGCTACATTCAATCTCATCCTCTATATGCTTGCAACTCATGTTGTTGGAGCATTTTGGTACCTGTCTACCATAGAGCGAAAAACAATGTGTTGGCATGGACGCTGCCATTATTGTCCTTTGAACTGTAATTACGTTCTTATTGCAAATAATGCCGACGATTTTTGTTCTGAACAAGCGGAAAATGATTCAAAAACTTATGACTTTGGGGTATTCAAAGATGCCCTTCCTATTTTGAATTCAAGAGATTTCATTTGGAAAATCTCTTATTGCTTTTGGTGGAGTCTGCAGAAATTGAGCTCTTTTGGTCAAGACCTGAAAACCAGTGGACATTTGTTAGAAATCTACTTTGCAATCACCATTACCATTTCTGGCTTGGTATTATTTGCACTTCTTATAGGAAATTTGCAGACATATTTGCAGTCAACCATTAGAAGCAAGGAGAAGATTAGATTGAAGGAGCAAGATATTGAGAACTGCATGACTAACTATTCTATCCCAGATAAGTTGAAGACGCAAGTTAGAAAATGTAAATTGTACAAGCAGCGAGAAGCCAAAGGTGTCGGCGTTGAGCAGCTTCTCCAAAATCTTCCCAACCATATAAGTAGGGATATAAAAGAACACCTTTGCTCTACTTCACTTTTCAATGTGCTCTTAAAGCTTGATAACATGGACAAGATATTGTCGGATGCAATCTATGATTTGCTAAAGCCAAAGCGATATATTGAACAAACGTTCATTGTTCAAGAAGGAGGACCAGTAGATGAAGTGGTCTTCATAATTCAAGGCAAGGTATTGGTCAGTTCCAAAAAAGATAGCAAGCCAGAAGAGTTGAACCGGCTTACCAAAGGTCACTTTTATGGAGAGGAACTTGTAGATTAG

Protein sequence

MIMCNKVRRKFHGWWNNIGCNWLKSLGKMVGRDSTLFRIIVGRKRKLHSKNGRLAMLSVIATSVDPLFFYILFVNEDRRCIGFHDEWKTAAVILRSVIDFLYAILTAYYFHDGYPLLNDETDGVLVRARKFLLSSSRVDLLAFLPLPQLLALLVIPRSKGLHFIHAIRALNYVVLVQYLSRVIRIYSFLMKSGWSTILPQAAEARATFNLILYMLATHVVGAFWYLSTIERKTMCWHGRCHYCPLNCNYVLIANNADDFCSEQAENDSKTYDFGVFKDALPILNSRDFIWKISYCFWWSLQKLSSFGQDLKTSGHLLEIYFAITITISGLVLFALLIGNLQTYLQSTIRSKEKIRLKEQDIENCMTNYSIPDKLKTQVRKCKLYKQREAKGVGVEQLLQNLPNHISRDIKEHLCSTSLFNVLLKLDNMDKILSDAIYDLLKPKRYIEQTFIVQEGGPVDEVVFIIQGKVLVSSKKDSKPEELNRLTKGHFYGEELVD
Homology
BLAST of Spg027392 vs. NCBI nr
Match: XP_022141010.1 (cyclic nucleotide-gated ion channel 1-like [Momordica charantia])

HSP 1 Score: 508.4 bits (1308), Expect = 6.8e-140
Identity = 275/472 (58.26%), Postives = 337/472 (71.40%), Query Frame = 0

Query: 36  LFRIIVGRKRKLHSKNGRLAMLSVIATSVDPLFFYILFVNEDRRCIGFHDEWKTAAVILR 95
           L RI+      LH  N  LAML VIATS+DPLF YILFV+E +RC+ F  + +T AVILR
Sbjct: 68  LKRIVNRHPILLHLWNEILAMLCVIATSLDPLFCYILFVDEGKRCVTFDKKLRTIAVILR 127

Query: 96  SVIDFLYAILTAYYFHDGYPLLNDET-----DGVLVRARKFLLSSSRVDLLAFLPLPQLL 155
           S+ DFLY IL   +F  GY    +       DG   +A +FL S   VD+LA LPLPQ++
Sbjct: 128 SITDFLYIILIVCHFQFGYSSFYNANRGGVDDGNCTKAWRFLWSYFTVDVLAVLPLPQVV 187

Query: 156 ALLVIPRSKGLHFIHAIRALNYVVLVQYLSRVIRIYSFLMKSGW-STILPQAAEARATFN 215
            L VIPR KG  FI AIR+L Y++L QYL R+ RIY+FL K  W + ILP+ A A+A FN
Sbjct: 188 VLFVIPRFKGYEFICAIRSLKYILLAQYLPRIFRIYAFLKKVRWTANILPETAGAKAAFN 247

Query: 216 LILYMLATHVVGAFWYLSTIERKTMCWHGRCHYCPLNCNYVLIANNADDFCSEQAENDSK 275
           L LYMLA+HV+GAFWYL TIERKT CW  RC +C LNC Y     +AD  CSE+A++   
Sbjct: 248 LFLYMLASHVIGAFWYLFTIERKTTCWLERCQHCSLNCIYASGNASADSICSEEAKDGPN 307

Query: 276 TYDFGVFKDALPILNSRDFIWKISYCFWWSLQKLSSFGQDLKTSGHLLEIYFAITITISG 335
            +DFG+FKDAL I+ +R+F+ KISYC+WW LQKLSS GQDLKTS HL E+YFAITITISG
Sbjct: 308 NFDFGIFKDALRIVRARNFVRKISYCYWWGLQKLSSLGQDLKTSDHLWEVYFAITITISG 367

Query: 336 LVLFALLIGNLQTYLQSTIRSKEKIRLKEQDIENCMTNYSIPDKLKTQVRKCKLYKQREA 395
           LVLFALL+GNLQTYLQSTI   E++RLK QDIE  MT +S+P KLK +++K + +K RE 
Sbjct: 368 LVLFALLVGNLQTYLQSTIARLEEMRLKGQDIELWMTYHSLPPKLKKRIKKYERFKWRET 427

Query: 396 KGVGVEQLLQNLPNHISRDIKEHLCSTSLFNVLLKLDNMDKILSDAIYDLLKPKRYIEQT 455
           KGV VEQLL NLP  + RD K HLC +SL NV  KL NMD+ L +AI D LKP  YIE++
Sbjct: 428 KGVDVEQLLHNLPRDLRRDTKRHLCLSSLSNV-SKLQNMDEQLLNAICDYLKPVLYIERS 487

Query: 456 FIVQEGGPVDEVVFIIQGKVLVSSKKDSK----PEELNRLTKGHFYGEELVD 498
           FIVQEG P+DE+VFII+GKV++ SK+D +      E   LTKG FYGE L+D
Sbjct: 488 FIVQEGEPLDEMVFIIRGKVMIYSKRDGESGADSSESKWLTKGDFYGEGLLD 538

BLAST of Spg027392 vs. NCBI nr
Match: XP_038904375.1 (cyclic nucleotide-gated ion channel 1-like [Benincasa hispida] >XP_038904379.1 cyclic nucleotide-gated ion channel 1-like [Benincasa hispida] >XP_038904383.1 cyclic nucleotide-gated ion channel 1-like [Benincasa hispida] >XP_038904388.1 cyclic nucleotide-gated ion channel 1-like [Benincasa hispida])

HSP 1 Score: 507.7 bits (1306), Expect = 1.2e-139
Identity = 269/461 (58.35%), Postives = 332/461 (72.02%), Query Frame = 0

Query: 47  LHSKNGRLAMLSVIATSVDPLFFYILFVNEDRRCIGFHDEWKTAAVILRSVIDFLYAILT 106
           LH  N  L ML VIATS+DP F YIL V+ED+RC+GF  + +T  VILRS+ DFLY IL 
Sbjct: 79  LHLWNEILVMLCVIATSLDPFFCYILLVDEDKRCVGFDKKLRTVVVILRSITDFLYIILI 138

Query: 107 AYYFHDGYPLL-----NDETDGVLVRARKFLLSSSRVDLLAFLPLPQLLALLVIPRSKGL 166
             +FH GY        ND   GV  RA +FL S   VD+L+ LPLPQ++ L+ IP  K  
Sbjct: 139 VCHFHFGYSSFYNANPNDPECGVCTRAWRFLFSYFTVDVLSVLPLPQVVVLVAIPSFKRY 198

Query: 167 HFIHAIRALNYVVLVQYLSRVIRIYSFLMKSGW-STILPQAAEARATFNLILYMLATHVV 226
            FI+A+R+L Y+++VQYL RV RIYSFL K  W S+ILP+ A A+A FNL LYMLA+HV+
Sbjct: 199 GFIYAVRSLKYILIVQYLPRVFRIYSFLKKVRWTSSILPETAGAKAAFNLFLYMLASHVI 258

Query: 227 GAFWYLSTIERKTMCWHGRCHYCPLNCNYVLIANNADDFCSEQAENDSKTYDFGVFKDAL 286
           GAFWYL TIERKT CW  R  + PLNC YV+   +AD+FCS +AEN + ++DFG+FK+AL
Sbjct: 259 GAFWYLFTIERKTTCWEERYPHWPLNCKYVVGNLSADNFCSPKAENAANSFDFGIFKEAL 318

Query: 287 PILNSRDFIWKISYCFWWSLQKLSSFGQDLKTSGHLLEIYFAITITISGLVLFALLIGNL 346
           PI+ S+DFI K S C+WW LQKLSS GQDLKTS HL E+YFA+TIT+SGLVLFALL+GNL
Sbjct: 319 PIVESKDFIKKTSLCYWWGLQKLSSLGQDLKTSYHLWEVYFAVTITVSGLVLFALLVGNL 378

Query: 347 QTYLQSTIRSKEKIRLKEQDIENCMTNYSIPDKLKTQVRKCKLYKQREAKGVGVEQLLQN 406
           QTYLQSTI   E++RLK QDIE  M  +S+P KLK +++K + YK RE KGV VEQLL N
Sbjct: 379 QTYLQSTIARLEEMRLKGQDIELWMAYHSLPPKLKKKIKKYERYKWRETKGVDVEQLLHN 438

Query: 407 LPNHISRDIKEHLCSTSLFNVLLKLDNMDKILSDAIYDLLKPKRYIEQTFIVQEGGPVDE 466
           LP  + RD K HLC T L +V  K  NMD  L +AI D LKP  YIE+  IVQEG P+DE
Sbjct: 439 LPRDLRRDTKRHLCLTPLLSV-SKFQNMDDKLLNAICDYLKPVLYIERNLIVQEGEPLDE 498

Query: 467 VVFIIQGKVLVSSKKDSK----PEELNRLTKGHFYGEELVD 498
           +VFII+GKV++ SK+DS+          LTKG FYGE+L+D
Sbjct: 499 IVFIIRGKVMIYSKRDSEGAAGSSGSKWLTKGDFYGEDLLD 538

BLAST of Spg027392 vs. NCBI nr
Match: XP_022968617.1 (cyclic nucleotide-gated ion channel 1-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 505.0 bits (1299), Expect = 7.5e-139
Identity = 269/462 (58.23%), Postives = 336/462 (72.73%), Query Frame = 0

Query: 47  LHSKNGRLAMLSVIATSVDPLFFYILFVNEDRRCIGFHDEWKTAAVILRSVIDFLYAILT 106
           LH  N  L ML VIATS+DPLF YIL V+ED+RC+GF  + +  AVILRS+ D LY IL 
Sbjct: 75  LHLWNEILVMLCVIATSLDPLFCYILVVDEDKRCVGFDKKLRKVAVILRSITDLLYIILI 134

Query: 107 AYYFHDGYPLL-----NDETDGVLVRARKFLLSSSRVDLLAFLPLPQLLALLVIPRSKGL 166
             +FH GY        +D  DGV  RA +FLLS   VD+LA LPLPQ+++L++IP  KG 
Sbjct: 135 VCHFHLGYSSFYKANSDDADDGVCTRAWRFLLSYFTVDVLAVLPLPQVVSLILIPSLKGY 194

Query: 167 HFIHAIRALNYVVLVQYLSRVIRIYSFLMKSGWST-ILPQAAEARATFNLILYMLATHVV 226
            FI+A+R+L Y+++VQYL RV RIYSFL K  WS+ ILP+ A A+A FNL LYMLA+HV+
Sbjct: 195 GFIYAVRSLKYILVVQYLPRVFRIYSFLKKVRWSSNILPETAGAKAAFNLFLYMLASHVI 254

Query: 227 GAFWYLSTIERKTMCWHGRCHYCPLNCNYVLIAN-NADDFCSEQAENDSKTYDFGVFKDA 286
           GAFWYL TIERKT+CW  RC +CPLNCNYV   N +AD FCS +A N S+T+DFG+F+  
Sbjct: 255 GAFWYLFTIERKTVCWEERCVHCPLNCNYVTNGNSSADSFCSGKAGNGSQTFDFGMFEAG 314

Query: 287 LPILNSRDFIWKISYCFWWSLQKLSSFGQDLKTSGHLLEIYFAITITISGLVLFALLIGN 346
           L I+ SR+F+ K+S+C+WW LQKLSS GQDLKTS HL EIYFA+TITISGL+LFALL+GN
Sbjct: 315 LRIVESRNFMRKLSFCYWWGLQKLSSLGQDLKTSDHLWEIYFAVTITISGLILFALLVGN 374

Query: 347 LQTYLQSTIRSKEKIRLKEQDIENCMTNYSIPDKLKTQVRKCKLYKQREAKGVGVEQLLQ 406
           LQTYLQSTI   E++RLK QDIE  M  +S+P KLK +++K + YK RE KGV VE LL 
Sbjct: 375 LQTYLQSTIARLEEMRLKGQDIELWMAFHSLPRKLKKRIKKYERYKWRETKGVDVELLLH 434

Query: 407 NLPNHISRDIKEHLCSTSLFNVLLKLDNMDKILSDAIYDLLKPKRYIEQTFIVQEGGPVD 466
           NLP  + RD K HLCST L +V  K  NMD  L +AI D L+P  YIE++ I+QEG P+D
Sbjct: 435 NLPRDLRRDTKRHLCSTPLKSV-PKFQNMDDKLLNAICDCLRPVLYIERSLIIQEGEPLD 494

Query: 467 EVVFIIQGKVLVSSKKD----SKPEELNRLTKGHFYGEELVD 498
           E+VFII+G+V + SK D    +     N L KG FYGE L++
Sbjct: 495 EMVFIIRGQVRIYSKSDNGIAASSSLFNCLNKGDFYGEALLE 535

BLAST of Spg027392 vs. NCBI nr
Match: XP_022968616.1 (cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 505.0 bits (1299), Expect = 7.5e-139
Identity = 269/462 (58.23%), Postives = 336/462 (72.73%), Query Frame = 0

Query: 47  LHSKNGRLAMLSVIATSVDPLFFYILFVNEDRRCIGFHDEWKTAAVILRSVIDFLYAILT 106
           LH  N  L ML VIATS+DPLF YIL V+ED+RC+GF  + +  AVILRS+ D LY IL 
Sbjct: 86  LHLWNEILVMLCVIATSLDPLFCYILVVDEDKRCVGFDKKLRKVAVILRSITDLLYIILI 145

Query: 107 AYYFHDGYPLL-----NDETDGVLVRARKFLLSSSRVDLLAFLPLPQLLALLVIPRSKGL 166
             +FH GY        +D  DGV  RA +FLLS   VD+LA LPLPQ+++L++IP  KG 
Sbjct: 146 VCHFHLGYSSFYKANSDDADDGVCTRAWRFLLSYFTVDVLAVLPLPQVVSLILIPSLKGY 205

Query: 167 HFIHAIRALNYVVLVQYLSRVIRIYSFLMKSGWST-ILPQAAEARATFNLILYMLATHVV 226
            FI+A+R+L Y+++VQYL RV RIYSFL K  WS+ ILP+ A A+A FNL LYMLA+HV+
Sbjct: 206 GFIYAVRSLKYILVVQYLPRVFRIYSFLKKVRWSSNILPETAGAKAAFNLFLYMLASHVI 265

Query: 227 GAFWYLSTIERKTMCWHGRCHYCPLNCNYVLIAN-NADDFCSEQAENDSKTYDFGVFKDA 286
           GAFWYL TIERKT+CW  RC +CPLNCNYV   N +AD FCS +A N S+T+DFG+F+  
Sbjct: 266 GAFWYLFTIERKTVCWEERCVHCPLNCNYVTNGNSSADSFCSGKAGNGSQTFDFGMFEAG 325

Query: 287 LPILNSRDFIWKISYCFWWSLQKLSSFGQDLKTSGHLLEIYFAITITISGLVLFALLIGN 346
           L I+ SR+F+ K+S+C+WW LQKLSS GQDLKTS HL EIYFA+TITISGL+LFALL+GN
Sbjct: 326 LRIVESRNFMRKLSFCYWWGLQKLSSLGQDLKTSDHLWEIYFAVTITISGLILFALLVGN 385

Query: 347 LQTYLQSTIRSKEKIRLKEQDIENCMTNYSIPDKLKTQVRKCKLYKQREAKGVGVEQLLQ 406
           LQTYLQSTI   E++RLK QDIE  M  +S+P KLK +++K + YK RE KGV VE LL 
Sbjct: 386 LQTYLQSTIARLEEMRLKGQDIELWMAFHSLPRKLKKRIKKYERYKWRETKGVDVELLLH 445

Query: 407 NLPNHISRDIKEHLCSTSLFNVLLKLDNMDKILSDAIYDLLKPKRYIEQTFIVQEGGPVD 466
           NLP  + RD K HLCST L +V  K  NMD  L +AI D L+P  YIE++ I+QEG P+D
Sbjct: 446 NLPRDLRRDTKRHLCSTPLKSV-PKFQNMDDKLLNAICDCLRPVLYIERSLIIQEGEPLD 505

Query: 467 EVVFIIQGKVLVSSKKD----SKPEELNRLTKGHFYGEELVD 498
           E+VFII+G+V + SK D    +     N L KG FYGE L++
Sbjct: 506 EMVFIIRGQVRIYSKSDNGIAASSSLFNCLNKGDFYGEALLE 546

BLAST of Spg027392 vs. NCBI nr
Match: XP_023541623.1 (cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 504.2 bits (1297), Expect = 1.3e-138
Identity = 268/462 (58.01%), Postives = 337/462 (72.94%), Query Frame = 0

Query: 47  LHSKNGRLAMLSVIATSVDPLFFYILFVNEDRRCIGFHDEWKTAAVILRSVIDFLYAILT 106
           LH  N  L ML VIATS+DPLF YIL V++D+RC+GF  + +  AVILRS+ D LY IL 
Sbjct: 86  LHLWNEILVMLCVIATSLDPLFCYILVVDDDKRCVGFDKKLRKVAVILRSITDLLYIILI 145

Query: 107 AYYFHDGYPLL-----NDETDGVLVRARKFLLSSSRVDLLAFLPLPQLLALLVIPRSKGL 166
             +FH GY        +D  DGV  RA +FLLS   VD+LA LPLPQ+++L++IP  KG 
Sbjct: 146 VCHFHLGYSSFYNANRDDADDGVCTRAWRFLLSYFTVDVLAVLPLPQVVSLILIPSLKGY 205

Query: 167 HFIHAIRALNYVVLVQYLSRVIRIYSFLMKSGWST-ILPQAAEARATFNLILYMLATHVV 226
            FI+A+R+L Y+++VQYL RV RIYSFL K  WS+ ILP+ A A+A FNL LYMLA+HV+
Sbjct: 206 GFIYAVRSLKYILVVQYLPRVFRIYSFLKKVRWSSNILPETAGAKAAFNLFLYMLASHVI 265

Query: 227 GAFWYLSTIERKTMCWHGRCHYCPLNCNYVLIAN-NADDFCSEQAENDSKTYDFGVFKDA 286
           GAFWYL TIERKT+CW  RC +CPLNC+Y    N +AD FCS ++ N+S+T+DFG+F+  
Sbjct: 266 GAFWYLFTIERKTVCWEERCVHCPLNCDYATNGNSSADSFCSGKSGNESQTFDFGMFEVG 325

Query: 287 LPILNSRDFIWKISYCFWWSLQKLSSFGQDLKTSGHLLEIYFAITITISGLVLFALLIGN 346
           L I+ SR+F+ KIS+C+WW LQKLSS GQDLKTS HL EIYFA+TITISGL+LFALL+GN
Sbjct: 326 LRIVKSRNFMRKISFCYWWGLQKLSSLGQDLKTSDHLWEIYFAVTITISGLILFALLVGN 385

Query: 347 LQTYLQSTIRSKEKIRLKEQDIENCMTNYSIPDKLKTQVRKCKLYKQREAKGVGVEQLLQ 406
           LQTYLQSTI   E++RLK QDIE  M  +S+P KLK +++K + YK RE KGV VE LL 
Sbjct: 386 LQTYLQSTIARLEEMRLKGQDIELWMAFHSLPRKLKKRIKKYERYKWRETKGVDVELLLH 445

Query: 407 NLPNHISRDIKEHLCSTSLFNVLLKLDNMDKILSDAIYDLLKPKRYIEQTFIVQEGGPVD 466
           NLP  + RD K HLCST L +V  K  NMD  L DAI D L+P  YIE++ I+QEG P+D
Sbjct: 446 NLPRDLRRDTKRHLCSTPLKSV-PKFQNMDDKLLDAICDCLRPVLYIERSLIIQEGEPLD 505

Query: 467 EVVFIIQGKVLVSSKKD----SKPEELNRLTKGHFYGEELVD 498
           E+VFII+G+V + SK D    +     N LTKG FYGE L++
Sbjct: 506 EMVFIIRGQVRIYSKSDNGIAASSTLFNCLTKGDFYGEALLE 546

BLAST of Spg027392 vs. ExPASy Swiss-Prot
Match: O65717 (Cyclic nucleotide-gated ion channel 1 OS=Arabidopsis thaliana OX=3702 GN=CNGC1 PE=1 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 3.5e-91
Identity = 196/467 (41.97%), Postives = 284/467 (60.81%), Query Frame = 0

Query: 51  NGRLAMLSVIATSVDPLFFYILFVNEDRRCIGFHDEWKTAAVILRSVIDFLYAILTAYYF 110
           N    +  +IA S+DPLFFY+  +++ ++C+G   + +  A +LRS  D  Y +   + F
Sbjct: 96  NKIFVLACIIAVSLDPLFFYVPIIDDAKKCLGIDKKMEITASVLRSFTDVFYVLHIIFQF 155

Query: 111 HDGY--PLLNDETDGVLVR-----ARKFLLSSSRVDLLAFLPLPQLLALLVIPRSKGLHF 170
             G+  P       GVLV      A+++L S   +D+LA LPLPQ++ L++IP  +G   
Sbjct: 156 RTGFIAPSSRVFGRGVLVEDKREIAKRYLSSHFIIDILAVLPLPQMVILIIIPHMRGSSS 215

Query: 171 IHAIRALNYVVLVQYLSRVIRIYSFLMK-SGWSTILPQAAEARATFNLILYMLATHVVGA 230
           ++    L ++V  QY+ R IRIY    + +  S IL + A A A FNL LYMLA+HV GA
Sbjct: 216 LNTKNMLKFIVFFQYIPRFIRIYPLYKEVTRTSGILTETAWAGAAFNLFLYMLASHVFGA 275

Query: 231 FWYLSTIERKTMCWHGRCHY----C---PLNCNYVLIANNA--DDFCSEQAENDSKTYDF 290
           FWYL +IER+T+CW   C      C    L C+      NA  ++ C  Q  N +  +DF
Sbjct: 276 FWYLFSIERETVCWKQACERNNPPCISKLLYCDPETAGGNAFLNESCPIQTPN-TTLFDF 335

Query: 291 GVFKDALP--ILNSRDFIWKISYCFWWSLQKLSSFGQDLKTSGHLLEIYFAITITISGLV 350
           G+F DAL   ++ S+DF  K  YCFWW LQ LSS GQ+LKTS ++ EI FA+ I+I+GLV
Sbjct: 336 GIFLDALQSGVVESQDFPQKFFYCFWWGLQNLSSLGQNLKTSTYIWEICFAVFISIAGLV 395

Query: 351 LFALLIGNLQTYLQSTIRSKEKIRLKEQDIENCMTNYSIPDKLKTQVRKCKLYKQREAKG 410
           LF+ LIGN+QTYLQST    E++R+K +D E  M++  +P+ L+ ++R+ + YK +E +G
Sbjct: 396 LFSFLIGNMQTYLQSTTTRLEEMRVKRRDAEQWMSHRLLPENLRKRIRRYEQYKWQETRG 455

Query: 411 VGVEQLLQNLPNHISRDIKEHLCSTSLFNVLLKLDNMDKILSDAIYDLLKPKRYIEQTFI 470
           V  E LL NLP  + RDIK HLC   L  V +  + MD+ L DA+ D L+P  Y E+++I
Sbjct: 456 VDEENLLSNLPKDLRRDIKRHLCLALLMRVPM-FEKMDEQLLDALCDRLQPVLYTEESYI 515

Query: 471 VQEGGPVDEVVFIIQGKVLVSSKKDSKPEELNR--LTKGHFYGEELV 497
           V+EG PVDE++FI++GK+L  +    +   LN   L  G F GEEL+
Sbjct: 516 VREGDPVDEMLFIMRGKLLTITTNGGRTGFLNSEYLGAGDFCGEELL 560

BLAST of Spg027392 vs. ExPASy Swiss-Prot
Match: Q9LD40 (Putative cyclic nucleotide-gated ion channel 13 OS=Arabidopsis thaliana OX=3702 GN=CNGC13 PE=3 SV=2)

HSP 1 Score: 304.7 bits (779), Expect = 1.9e-81
Identity = 187/474 (39.45%), Postives = 273/474 (57.59%), Query Frame = 0

Query: 47  LHSKNGRLAMLSVIATSVDPLFFYILFVNEDRRCIGFHDEWKTAAVILRSVIDFLYAILT 106
           L + N      SVIA ++DPLFFYI  V+ +R C+  H   + AA +LR+ ID  Y I  
Sbjct: 76  LQNWNKIFLFASVIALAIDPLFFYIPIVDGERHCLNLHRNLEIAASVLRTFIDAFYIIHI 135

Query: 107 AYYFHDGY--PLLNDETDGVLVR-----ARKFLLSSSRVDLLAFLPLPQLLALLVIPRSK 166
            + F   Y  P       G LV      A K+L S   +DLL+ LPLPQL+ L VIP   
Sbjct: 136 VFQFRTAYISPSSRVFGRGELVDDPKAIAIKYLSSYFIIDLLSILPLPQLVVLAVIPNVN 195

Query: 167 GLHFIHAIRALNYVVLVQYLSRVIRIYSFLMK-SGWSTILPQAAEARATFNLILYMLATH 226
               +     L  V+  QY+ R++RIY    + +  S I+ + A A A +NL LYMLA+H
Sbjct: 196 KPVSLITKDYLITVIFTQYIPRILRIYPLYTEVTRTSGIVTETAWAGAAWNLSLYMLASH 255

Query: 227 VVGAFWYLSTIERKTMCWHGRCHYCPLNCNYVLI-----ANNADDFCSEQAE-------N 286
           V GA WYL ++ER+  CW   C   P  CN+  +     ++  +DF +            
Sbjct: 256 VFGALWYLISVEREDRCWREACEKIPEVCNFRFLYCDGNSSVRNDFLTTSCPFINPDDIT 315

Query: 287 DSKTYDFGVFKDALP--ILNSRDFIWKISYCFWWSLQKLSSFGQDLKTSGHLLEIYFAIT 346
           +S  ++FG+F DAL   I+ S DF  K  YCFWW L+ LS+ GQ+L TS  + EI FA++
Sbjct: 316 NSTVFNFGIFTDALKSGIVESDDFWKKFFYCFWWGLRNLSALGQNLNTSKFVGEIIFAVS 375

Query: 347 ITISGLVLFALLIGNLQTYLQSTIRSKEKIRLKEQDIENCMTNYSIPDKLKTQVRKCKLY 406
           I ISGLVLFALLIGN+Q YL+ST   +E++R++++D E  M++  +PD L+ ++R+ + Y
Sbjct: 376 ICISGLVLFALLIGNMQKYLESTTVREEEMRVRKRDAEQWMSHRMLPDDLRKRIRRYEQY 435

Query: 407 KQREAKGVGVEQLLQNLPNHISRDIKEHLCSTSLFNVLLKLDNMDKILSDAIYDLLKPKR 466
           K +E +GV  E LL+NLP  + RDIK H C   L  V L  + MD+ L DA+ D LKP  
Sbjct: 436 KWQETRGVEEENLLRNLPKDLRRDIKRHFCLDLLKKVPL-FEIMDEQLLDAVCDKLKPVL 495

Query: 467 YIEQTFIVQEGGPVDEVVFIIQGKVLVSSKKDSKPEELNR--LTKGHFYGEELV 497
           Y E ++ ++EG PV+E++F+++GK++ ++    +    N   L    F GE+L+
Sbjct: 496 YTENSYAIREGDPVEEMLFVMRGKLMSATTNGGRTGFFNAVYLKPSDFCGEDLL 548

BLAST of Spg027392 vs. ExPASy Swiss-Prot
Match: Q9SKD7 (Probable cyclic nucleotide-gated ion channel 3 OS=Arabidopsis thaliana OX=3702 GN=CNGC3 PE=2 SV=2)

HSP 1 Score: 301.6 bits (771), Expect = 1.6e-80
Identity = 186/486 (38.27%), Postives = 273/486 (56.17%), Query Frame = 0

Query: 36  LFRIIVGRKRKLHSKNGRLAMLSVIATSVDPLFFYILFVNEDRRCIGFHDEWKTAAVILR 95
           + R++      L S N    +LSV+A + DPLFFYI +V  +R C+    + +T A + R
Sbjct: 69  ILRVMNPNDSYLQSWNKIFLLLSVVALAFDPLFFYIPYVKPERFCLNLDKKLQTIACVFR 128

Query: 96  SVIDFLYAILTAYYFHDGYPL----------LNDETDGVLVRARKFLLSSSRVDLLAFLP 155
           + ID  Y +   + FH G+            LN++   + +R   +L S   +DLL+ LP
Sbjct: 129 TFIDAFYVVHMLFQFHTGFITPSSSGFGRGELNEKHKDIALR---YLGSYFLIDLLSILP 188

Query: 156 LPQLLALLVIPRSKGLHFIHAIRALNYVVLVQYLSRVIRIYS-FLMKSGWSTILPQAAEA 215
           +PQ++ L ++PR +    + A   L +V+  QY+ R+ RIY  F   +  S ++ + A A
Sbjct: 189 IPQVVVLAIVPRMRRPASLVAKELLKWVIFCQYVPRIARIYPLFKEVTRTSGLVTETAWA 248

Query: 216 RATFNLILYMLATHVVGAFWYLSTIERKTMCWHGRCH----------YC-PLNCNYVLIA 275
            A  NL LYMLA+HV G+FWYL +IERK  CW   C           YC P   +  L  
Sbjct: 249 GAALNLFLYMLASHVFGSFWYLISIERKDRCWREACAKIQNCTHAYLYCSPTGEDNRLFL 308

Query: 276 NNADDFCSEQAENDSKTYDFGVFKDALP--ILNSRDFIWKISYCFWWSLQKLSSFGQDLK 335
           N +      +   +S  ++FG+F DAL   ++ SRDF  K  YCFWW L+ LS+ GQ+LK
Sbjct: 309 NGSCPLIDPEEITNSTVFNFGIFADALQSGVVESRDFPKKFFYCFWWGLRNLSALGQNLK 368

Query: 336 TSGHLLEIYFAITITISGLVLFALLIGNLQTYLQSTIRSKEKIRLKEQDIENCMTNYSIP 395
           TS    EI FAI I ISGLVLFALLIGN+Q YLQST    E++R+K +D E  M++  +P
Sbjct: 369 TSAFEGEIIFAIVICISGLVLFALLIGNMQKYLQSTTVRVEEMRVKRRDAEQWMSHRMLP 428

Query: 396 DKLKTQVRKCKLYKQREAKGVGVEQLLQNLPNHISRDIKEHLCSTSLFNVLLKLDNMDKI 455
           D L+ ++RK + YK +E KGV  E LL +LP  + +DIK HLC   L   +     MD  
Sbjct: 429 DDLRKRIRKYEQYKWQETKGVEEEALLSSLPKDLRKDIKRHLC-LKLLKKVPWFQAMDDR 488

Query: 456 LSDAIYDLLKPKRYIEQTFIVQEGGPVDEVVFIIQGKVLVSSKKDSKPEELNR--LTKGH 496
           L DA+   LK   Y E+++IV+EG PV++++FI++G ++ ++    +    N   L  G 
Sbjct: 489 LLDALCARLKTVLYTEKSYIVREGEPVEDMLFIMRGNLISTTTYGGRTGFFNSVDLVAGD 548

BLAST of Spg027392 vs. ExPASy Swiss-Prot
Match: Q9LNJ0 (Probable cyclic nucleotide-gated ion channel 10 OS=Arabidopsis thaliana OX=3702 GN=CNGC10 PE=2 SV=2)

HSP 1 Score: 290.0 bits (741), Expect = 4.9e-77
Identity = 174/464 (37.50%), Postives = 269/464 (57.97%), Query Frame = 0

Query: 59  VIATSVDPLFFYILFVNEDRRCIGFHDEWKTAAVILRSVIDFLYAILTAYYFHDGYPLLN 118
           V+A ++DPLFFYI  V+  R C+    + + AA +LR++ID  Y I   + F   Y   +
Sbjct: 88  VVALAIDPLFFYIPIVDSARHCLTLDSKLEIAASLLRTLIDAFYIIHIVFQFRTAYIAPS 147

Query: 119 DE-------TDGVLVRARKFLLSSSRVDLLAFLPLPQLLALLVIPRSKGLHFIHAIRALN 178
                     D     A K+L S   +DLL+ LPLPQ++ L VIP       +     L 
Sbjct: 148 SRVFGRGELVDDAKAIALKYLSSYFIIDLLSILPLPQIVVLAVIPSVNQPVSLLTKDYLK 207

Query: 179 YVVLVQYLSRVIRIYSFLMK-SGWSTILPQAAEARATFNLILYMLATHVVGAFWYLSTIE 238
           + ++ QY+ R++R+Y    + +  S I+ + A A A +NL LYMLA+HV GA WYL ++E
Sbjct: 208 FSIIAQYVPRILRMYPLYTEVTRTSGIVTETAWAGAAWNLSLYMLASHVFGALWYLISVE 267

Query: 239 RKTMCWHGRCH----------YCPLNCNYVLIANN----ADDFCSEQAENDSKTYDFGVF 298
           R+  CW   C           YC  + N   ++NN    +  F       +S  ++FG+F
Sbjct: 268 REDRCWQEACEKTKGCNMKFLYCENDRN---VSNNFLTTSCPFLDPGDITNSTIFNFGIF 327

Query: 299 KDALP--ILNSRDFIWKISYCFWWSLQKLSSFGQDLKTSGHLLEIYFAITITISGLVLFA 358
            DAL   ++ S DF  K  YCFWW L+ LS+ GQ+L+TS  + EI FAI+I ISGLVLFA
Sbjct: 328 TDALKSGVVESHDFWKKFFYCFWWGLRNLSALGQNLQTSKFVGEIIFAISICISGLVLFA 387

Query: 359 LLIGNLQTYLQSTIRSKEKIRLKEQDIENCMTNYSIPDKLKTQVRKCKLYKQREAKGVGV 418
           LLIGN+Q YL+ST   +E++R++++D E  M++  +P+ L+ ++R+ + Y+ +E +GV  
Sbjct: 388 LLIGNMQKYLESTTVREEEMRVRKRDAEQWMSHRMLPEDLRKRIRRYEQYRWQETRGVEE 447

Query: 419 EQLLQNLPNHISRDIKEHLCSTSLFNVLLKLDNMDKILSDAIYDLLKPKRYIEQTFIVQE 478
           E LL+NLP  + RDIK HLC   L  V L  + MD+ L DA+ D L+P  Y E +++++E
Sbjct: 448 ETLLRNLPKDLRRDIKRHLCLDLLKKVPL-FEIMDEQLLDAVCDRLRPVLYTENSYVIRE 507

Query: 479 GGPVDEVVFIIQGKVLVSSKKDSKPEELN--RLTKGHFYGEELV 497
           G PV E++F+++G+++ ++    +    N   L    F GE+L+
Sbjct: 508 GDPVGEMLFVMRGRLVSATTNGGRSGFFNAVNLKASDFCGEDLL 547

BLAST of Spg027392 vs. ExPASy Swiss-Prot
Match: O82226 (Probable cyclic nucleotide-gated ion channel 6 OS=Arabidopsis thaliana OX=3702 GN=CNGC6 PE=1 SV=2)

HSP 1 Score: 287.3 bits (734), Expect = 3.2e-76
Identity = 175/468 (37.39%), Postives = 269/468 (57.48%), Query Frame = 0

Query: 59  VIATSVDPLFFYILFVNEDRRCIGFHDEWKTAAVILRSVIDFLYAILTAYYFHDGY--PL 118
           ++A SVDPLF Y+ F+N+  +C+G   +       +R+VID  Y    A  F   Y  P 
Sbjct: 123 ILAVSVDPLFLYLPFINDKAKCVGIDRKLAIIVTTIRTVIDSFYLFHMALRFRTAYVAPS 182

Query: 119 LNDETDGVLV-----RARKFLLSSSRVDLLAFLPLPQLLALLVIPRSKGLHFIHAIRALN 178
                 G LV      A+++L     +DLL+ LP+PQ++    +  S+G + +   +AL 
Sbjct: 183 SRVFGRGELVIDPAQIAKRYLQQYFIIDLLSVLPVPQIIVWRFLYTSRGANVLATKQALR 242

Query: 179 YVVLVQYLSRVIRIYSFLMK-SGWSTILPQAAEARATFNLILYMLATHVVGAFWYLSTIE 238
           Y+VLVQY+ R +R+Y    +    + +  + A A A + L+LYMLA+H+VGA WYL  +E
Sbjct: 243 YIVLVQYIPRFLRMYPLSSELKRTAGVFAETAWAGAAYYLLLYMLASHIVGALWYLLALE 302

Query: 239 RKTMCWHGRCHYCPLNC--NYVLIAN-NADDFCS---------------EQAENDSKTYD 298
           R   CW   CH    NC  N++   N N   + +                  E++   +D
Sbjct: 303 RNNDCWSKACHN-NQNCTRNFLFCGNQNMKGYAAWDNIKVSYLQLKCPVNVPEDEEPPFD 362

Query: 299 FGVFKDALP--ILNSRDFIWKISYCFWWSLQKLSSFGQDLKTSGHLLEIYFAITITISGL 358
           FG++  AL   I++S++F+ K  +C WW LQ LS+ GQ L+TS +  E+ F+IT+ I+GL
Sbjct: 363 FGIYLRALSSGIVSSKNFVSKYFFCLWWGLQNLSTLGQGLETSTYPGEVIFSITLAIAGL 422

Query: 359 VLFALLIGNLQTYLQSTIRSKEKIRLKEQDIENCMTNYSIPDKLKTQVRKCKLYKQREAK 418
           +LFALLIGN+QTYLQS     E++R+K +D E  M +  +P +L+ +VR+   YK  E +
Sbjct: 423 LLFALLIGNMQTYLQSLTIRLEEMRVKRRDSEQWMHHRMLPPELRERVRRYDQYKWLETR 482

Query: 419 GVGVEQLLQNLPNHISRDIKEHLCSTSLFNVLLKLDNMDKILSDAIYDLLKPKRYIEQTF 478
           GV  E L+QNLP  + RDIK HLC   +  V L  +NMD+ L DAI + LKP  + E+++
Sbjct: 483 GVDEENLVQNLPKDLRRDIKRHLCLALVRRVPL-FENMDERLLDAICERLKPCLFTEKSY 542

Query: 479 IVQEGGPVDEVVFIIQGKVLVSSKKDSKPEELNR--LTKGHFYGEELV 497
           +V+EG PV+E++FII+G++   +    +    NR  L +G F G+EL+
Sbjct: 543 LVREGDPVNEMLFIIRGRLESVTTDGGRSGFYNRSLLKEGDFCGDELL 588

BLAST of Spg027392 vs. ExPASy TrEMBL
Match: A0A6J1CGR8 (cyclic nucleotide-gated ion channel 1-like OS=Momordica charantia OX=3673 GN=LOC111011521 PE=4 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 3.3e-140
Identity = 275/472 (58.26%), Postives = 337/472 (71.40%), Query Frame = 0

Query: 36  LFRIIVGRKRKLHSKNGRLAMLSVIATSVDPLFFYILFVNEDRRCIGFHDEWKTAAVILR 95
           L RI+      LH  N  LAML VIATS+DPLF YILFV+E +RC+ F  + +T AVILR
Sbjct: 68  LKRIVNRHPILLHLWNEILAMLCVIATSLDPLFCYILFVDEGKRCVTFDKKLRTIAVILR 127

Query: 96  SVIDFLYAILTAYYFHDGYPLLNDET-----DGVLVRARKFLLSSSRVDLLAFLPLPQLL 155
           S+ DFLY IL   +F  GY    +       DG   +A +FL S   VD+LA LPLPQ++
Sbjct: 128 SITDFLYIILIVCHFQFGYSSFYNANRGGVDDGNCTKAWRFLWSYFTVDVLAVLPLPQVV 187

Query: 156 ALLVIPRSKGLHFIHAIRALNYVVLVQYLSRVIRIYSFLMKSGW-STILPQAAEARATFN 215
            L VIPR KG  FI AIR+L Y++L QYL R+ RIY+FL K  W + ILP+ A A+A FN
Sbjct: 188 VLFVIPRFKGYEFICAIRSLKYILLAQYLPRIFRIYAFLKKVRWTANILPETAGAKAAFN 247

Query: 216 LILYMLATHVVGAFWYLSTIERKTMCWHGRCHYCPLNCNYVLIANNADDFCSEQAENDSK 275
           L LYMLA+HV+GAFWYL TIERKT CW  RC +C LNC Y     +AD  CSE+A++   
Sbjct: 248 LFLYMLASHVIGAFWYLFTIERKTTCWLERCQHCSLNCIYASGNASADSICSEEAKDGPN 307

Query: 276 TYDFGVFKDALPILNSRDFIWKISYCFWWSLQKLSSFGQDLKTSGHLLEIYFAITITISG 335
            +DFG+FKDAL I+ +R+F+ KISYC+WW LQKLSS GQDLKTS HL E+YFAITITISG
Sbjct: 308 NFDFGIFKDALRIVRARNFVRKISYCYWWGLQKLSSLGQDLKTSDHLWEVYFAITITISG 367

Query: 336 LVLFALLIGNLQTYLQSTIRSKEKIRLKEQDIENCMTNYSIPDKLKTQVRKCKLYKQREA 395
           LVLFALL+GNLQTYLQSTI   E++RLK QDIE  MT +S+P KLK +++K + +K RE 
Sbjct: 368 LVLFALLVGNLQTYLQSTIARLEEMRLKGQDIELWMTYHSLPPKLKKRIKKYERFKWRET 427

Query: 396 KGVGVEQLLQNLPNHISRDIKEHLCSTSLFNVLLKLDNMDKILSDAIYDLLKPKRYIEQT 455
           KGV VEQLL NLP  + RD K HLC +SL NV  KL NMD+ L +AI D LKP  YIE++
Sbjct: 428 KGVDVEQLLHNLPRDLRRDTKRHLCLSSLSNV-SKLQNMDEQLLNAICDYLKPVLYIERS 487

Query: 456 FIVQEGGPVDEVVFIIQGKVLVSSKKDSK----PEELNRLTKGHFYGEELVD 498
           FIVQEG P+DE+VFII+GKV++ SK+D +      E   LTKG FYGE L+D
Sbjct: 488 FIVQEGEPLDEMVFIIRGKVMIYSKRDGESGADSSESKWLTKGDFYGEGLLD 538

BLAST of Spg027392 vs. ExPASy TrEMBL
Match: A0A6J1HU01 (cyclic nucleotide-gated ion channel 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467780 PE=4 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 3.6e-139
Identity = 269/462 (58.23%), Postives = 336/462 (72.73%), Query Frame = 0

Query: 47  LHSKNGRLAMLSVIATSVDPLFFYILFVNEDRRCIGFHDEWKTAAVILRSVIDFLYAILT 106
           LH  N  L ML VIATS+DPLF YIL V+ED+RC+GF  + +  AVILRS+ D LY IL 
Sbjct: 75  LHLWNEILVMLCVIATSLDPLFCYILVVDEDKRCVGFDKKLRKVAVILRSITDLLYIILI 134

Query: 107 AYYFHDGYPLL-----NDETDGVLVRARKFLLSSSRVDLLAFLPLPQLLALLVIPRSKGL 166
             +FH GY        +D  DGV  RA +FLLS   VD+LA LPLPQ+++L++IP  KG 
Sbjct: 135 VCHFHLGYSSFYKANSDDADDGVCTRAWRFLLSYFTVDVLAVLPLPQVVSLILIPSLKGY 194

Query: 167 HFIHAIRALNYVVLVQYLSRVIRIYSFLMKSGWST-ILPQAAEARATFNLILYMLATHVV 226
            FI+A+R+L Y+++VQYL RV RIYSFL K  WS+ ILP+ A A+A FNL LYMLA+HV+
Sbjct: 195 GFIYAVRSLKYILVVQYLPRVFRIYSFLKKVRWSSNILPETAGAKAAFNLFLYMLASHVI 254

Query: 227 GAFWYLSTIERKTMCWHGRCHYCPLNCNYVLIAN-NADDFCSEQAENDSKTYDFGVFKDA 286
           GAFWYL TIERKT+CW  RC +CPLNCNYV   N +AD FCS +A N S+T+DFG+F+  
Sbjct: 255 GAFWYLFTIERKTVCWEERCVHCPLNCNYVTNGNSSADSFCSGKAGNGSQTFDFGMFEAG 314

Query: 287 LPILNSRDFIWKISYCFWWSLQKLSSFGQDLKTSGHLLEIYFAITITISGLVLFALLIGN 346
           L I+ SR+F+ K+S+C+WW LQKLSS GQDLKTS HL EIYFA+TITISGL+LFALL+GN
Sbjct: 315 LRIVESRNFMRKLSFCYWWGLQKLSSLGQDLKTSDHLWEIYFAVTITISGLILFALLVGN 374

Query: 347 LQTYLQSTIRSKEKIRLKEQDIENCMTNYSIPDKLKTQVRKCKLYKQREAKGVGVEQLLQ 406
           LQTYLQSTI   E++RLK QDIE  M  +S+P KLK +++K + YK RE KGV VE LL 
Sbjct: 375 LQTYLQSTIARLEEMRLKGQDIELWMAFHSLPRKLKKRIKKYERYKWRETKGVDVELLLH 434

Query: 407 NLPNHISRDIKEHLCSTSLFNVLLKLDNMDKILSDAIYDLLKPKRYIEQTFIVQEGGPVD 466
           NLP  + RD K HLCST L +V  K  NMD  L +AI D L+P  YIE++ I+QEG P+D
Sbjct: 435 NLPRDLRRDTKRHLCSTPLKSV-PKFQNMDDKLLNAICDCLRPVLYIERSLIIQEGEPLD 494

Query: 467 EVVFIIQGKVLVSSKKD----SKPEELNRLTKGHFYGEELVD 498
           E+VFII+G+V + SK D    +     N L KG FYGE L++
Sbjct: 495 EMVFIIRGQVRIYSKSDNGIAASSSLFNCLNKGDFYGEALLE 535

BLAST of Spg027392 vs. ExPASy TrEMBL
Match: A0A6J1HVE3 (cyclic nucleotide-gated ion channel 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467780 PE=4 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 3.6e-139
Identity = 269/462 (58.23%), Postives = 336/462 (72.73%), Query Frame = 0

Query: 47  LHSKNGRLAMLSVIATSVDPLFFYILFVNEDRRCIGFHDEWKTAAVILRSVIDFLYAILT 106
           LH  N  L ML VIATS+DPLF YIL V+ED+RC+GF  + +  AVILRS+ D LY IL 
Sbjct: 86  LHLWNEILVMLCVIATSLDPLFCYILVVDEDKRCVGFDKKLRKVAVILRSITDLLYIILI 145

Query: 107 AYYFHDGYPLL-----NDETDGVLVRARKFLLSSSRVDLLAFLPLPQLLALLVIPRSKGL 166
             +FH GY        +D  DGV  RA +FLLS   VD+LA LPLPQ+++L++IP  KG 
Sbjct: 146 VCHFHLGYSSFYKANSDDADDGVCTRAWRFLLSYFTVDVLAVLPLPQVVSLILIPSLKGY 205

Query: 167 HFIHAIRALNYVVLVQYLSRVIRIYSFLMKSGWST-ILPQAAEARATFNLILYMLATHVV 226
            FI+A+R+L Y+++VQYL RV RIYSFL K  WS+ ILP+ A A+A FNL LYMLA+HV+
Sbjct: 206 GFIYAVRSLKYILVVQYLPRVFRIYSFLKKVRWSSNILPETAGAKAAFNLFLYMLASHVI 265

Query: 227 GAFWYLSTIERKTMCWHGRCHYCPLNCNYVLIAN-NADDFCSEQAENDSKTYDFGVFKDA 286
           GAFWYL TIERKT+CW  RC +CPLNCNYV   N +AD FCS +A N S+T+DFG+F+  
Sbjct: 266 GAFWYLFTIERKTVCWEERCVHCPLNCNYVTNGNSSADSFCSGKAGNGSQTFDFGMFEAG 325

Query: 287 LPILNSRDFIWKISYCFWWSLQKLSSFGQDLKTSGHLLEIYFAITITISGLVLFALLIGN 346
           L I+ SR+F+ K+S+C+WW LQKLSS GQDLKTS HL EIYFA+TITISGL+LFALL+GN
Sbjct: 326 LRIVESRNFMRKLSFCYWWGLQKLSSLGQDLKTSDHLWEIYFAVTITISGLILFALLVGN 385

Query: 347 LQTYLQSTIRSKEKIRLKEQDIENCMTNYSIPDKLKTQVRKCKLYKQREAKGVGVEQLLQ 406
           LQTYLQSTI   E++RLK QDIE  M  +S+P KLK +++K + YK RE KGV VE LL 
Sbjct: 386 LQTYLQSTIARLEEMRLKGQDIELWMAFHSLPRKLKKRIKKYERYKWRETKGVDVELLLH 445

Query: 407 NLPNHISRDIKEHLCSTSLFNVLLKLDNMDKILSDAIYDLLKPKRYIEQTFIVQEGGPVD 466
           NLP  + RD K HLCST L +V  K  NMD  L +AI D L+P  YIE++ I+QEG P+D
Sbjct: 446 NLPRDLRRDTKRHLCSTPLKSV-PKFQNMDDKLLNAICDCLRPVLYIERSLIIQEGEPLD 505

Query: 467 EVVFIIQGKVLVSSKKD----SKPEELNRLTKGHFYGEELVD 498
           E+VFII+G+V + SK D    +     N L KG FYGE L++
Sbjct: 506 EMVFIIRGQVRIYSKSDNGIAASSSLFNCLNKGDFYGEALLE 546

BLAST of Spg027392 vs. ExPASy TrEMBL
Match: A0A6J1G2D2 (cyclic nucleotide-gated ion channel 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450022 PE=4 SV=1)

HSP 1 Score: 502.7 bits (1293), Expect = 1.8e-138
Identity = 269/462 (58.23%), Postives = 337/462 (72.94%), Query Frame = 0

Query: 47  LHSKNGRLAMLSVIATSVDPLFFYILFVNEDRRCIGFHDEWKTAAVILRSVIDFLYAILT 106
           LH  N  L ML VIATS+DPLF YIL V++D RC+GF  + +  AVILRS+ D LY IL 
Sbjct: 86  LHLWNEILVMLCVIATSLDPLFCYILVVDDDERCVGFDKKLRKVAVILRSITDLLYIILI 145

Query: 107 AYYFHDGYPLL-----NDETDGVLVRARKFLLSSSRVDLLAFLPLPQLLALLVIPRSKGL 166
             +FH GY        +D  DGV  RA +FLLS   VD+LA LPLPQ+++L++IP  KG 
Sbjct: 146 VCHFHLGYSSFYNANSDDPDDGVCTRAWRFLLSYFTVDVLAVLPLPQVVSLILIPSLKGH 205

Query: 167 HFIHAIRALNYVVLVQYLSRVIRIYSFLMKSGWST-ILPQAAEARATFNLILYMLATHVV 226
            FI+A+R+L Y+++VQYL RV RIYSFL K  WS+ ILP+ A A+A FNL LYMLA+HV+
Sbjct: 206 GFIYAVRSLKYILVVQYLPRVFRIYSFLKKVRWSSNILPETAGAKAAFNLFLYMLASHVI 265

Query: 227 GAFWYLSTIERKTMCWHGRCHYCPLNCNYVLIAN-NADDFCSEQAENDSKTYDFGVFKDA 286
           GAFWYL TIERKT+CW  RC +CPLNC+YV   N +AD FCS +A N+S+T+DFG+F+  
Sbjct: 266 GAFWYLFTIERKTVCWVERCVHCPLNCDYVTNGNSSADSFCSGKAGNESQTFDFGMFEVG 325

Query: 287 LPILNSRDFIWKISYCFWWSLQKLSSFGQDLKTSGHLLEIYFAITITISGLVLFALLIGN 346
           L I+ SR+F+ KIS+C+WW LQKLSS GQDLKTS HL EIYFA+TITISGL+LFALL+GN
Sbjct: 326 LRIVKSRNFMRKISFCYWWGLQKLSSLGQDLKTSDHLWEIYFAVTITISGLILFALLVGN 385

Query: 347 LQTYLQSTIRSKEKIRLKEQDIENCMTNYSIPDKLKTQVRKCKLYKQREAKGVGVEQLLQ 406
           LQTYLQSTI   E++RLK QDIE  M  +S+P KLK +++K + YK RE KGV VE LL 
Sbjct: 386 LQTYLQSTIARLEEMRLKGQDIELWMAFHSLPRKLKKRIKKYERYKWRETKGVDVELLLH 445

Query: 407 NLPNHISRDIKEHLCSTSLFNVLLKLDNMDKILSDAIYDLLKPKRYIEQTFIVQEGGPVD 466
           NLP  + RD K HLCST L +V  K  NMD  L +AI D L+P  YIE++ I+QEG P+D
Sbjct: 446 NLPRDLRRDTKRHLCSTPLKSV-PKFQNMDDKLLNAICDCLRPVLYIERSLIIQEGEPLD 505

Query: 467 EVVFIIQGKVLVSSKKD----SKPEELNRLTKGHFYGEELVD 498
           E+VFII+G+V + SK D    +     N LTKG FYGE L++
Sbjct: 506 EMVFIIRGQVRIYSKSDNGIAASSSLFNCLTKGDFYGEALLE 546

BLAST of Spg027392 vs. ExPASy TrEMBL
Match: A0A6J1G295 (cyclic nucleotide-gated ion channel 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111450022 PE=4 SV=1)

HSP 1 Score: 502.7 bits (1293), Expect = 1.8e-138
Identity = 269/462 (58.23%), Postives = 337/462 (72.94%), Query Frame = 0

Query: 47  LHSKNGRLAMLSVIATSVDPLFFYILFVNEDRRCIGFHDEWKTAAVILRSVIDFLYAILT 106
           LH  N  L ML VIATS+DPLF YIL V++D RC+GF  + +  AVILRS+ D LY IL 
Sbjct: 75  LHLWNEILVMLCVIATSLDPLFCYILVVDDDERCVGFDKKLRKVAVILRSITDLLYIILI 134

Query: 107 AYYFHDGYPLL-----NDETDGVLVRARKFLLSSSRVDLLAFLPLPQLLALLVIPRSKGL 166
             +FH GY        +D  DGV  RA +FLLS   VD+LA LPLPQ+++L++IP  KG 
Sbjct: 135 VCHFHLGYSSFYNANSDDPDDGVCTRAWRFLLSYFTVDVLAVLPLPQVVSLILIPSLKGH 194

Query: 167 HFIHAIRALNYVVLVQYLSRVIRIYSFLMKSGWST-ILPQAAEARATFNLILYMLATHVV 226
            FI+A+R+L Y+++VQYL RV RIYSFL K  WS+ ILP+ A A+A FNL LYMLA+HV+
Sbjct: 195 GFIYAVRSLKYILVVQYLPRVFRIYSFLKKVRWSSNILPETAGAKAAFNLFLYMLASHVI 254

Query: 227 GAFWYLSTIERKTMCWHGRCHYCPLNCNYVLIAN-NADDFCSEQAENDSKTYDFGVFKDA 286
           GAFWYL TIERKT+CW  RC +CPLNC+YV   N +AD FCS +A N+S+T+DFG+F+  
Sbjct: 255 GAFWYLFTIERKTVCWVERCVHCPLNCDYVTNGNSSADSFCSGKAGNESQTFDFGMFEVG 314

Query: 287 LPILNSRDFIWKISYCFWWSLQKLSSFGQDLKTSGHLLEIYFAITITISGLVLFALLIGN 346
           L I+ SR+F+ KIS+C+WW LQKLSS GQDLKTS HL EIYFA+TITISGL+LFALL+GN
Sbjct: 315 LRIVKSRNFMRKISFCYWWGLQKLSSLGQDLKTSDHLWEIYFAVTITISGLILFALLVGN 374

Query: 347 LQTYLQSTIRSKEKIRLKEQDIENCMTNYSIPDKLKTQVRKCKLYKQREAKGVGVEQLLQ 406
           LQTYLQSTI   E++RLK QDIE  M  +S+P KLK +++K + YK RE KGV VE LL 
Sbjct: 375 LQTYLQSTIARLEEMRLKGQDIELWMAFHSLPRKLKKRIKKYERYKWRETKGVDVELLLH 434

Query: 407 NLPNHISRDIKEHLCSTSLFNVLLKLDNMDKILSDAIYDLLKPKRYIEQTFIVQEGGPVD 466
           NLP  + RD K HLCST L +V  K  NMD  L +AI D L+P  YIE++ I+QEG P+D
Sbjct: 435 NLPRDLRRDTKRHLCSTPLKSV-PKFQNMDDKLLNAICDCLRPVLYIERSLIIQEGEPLD 494

Query: 467 EVVFIIQGKVLVSSKKD----SKPEELNRLTKGHFYGEELVD 498
           E+VFII+G+V + SK D    +     N LTKG FYGE L++
Sbjct: 495 EMVFIIRGQVRIYSKSDNGIAASSSLFNCLTKGDFYGEALLE 535

BLAST of Spg027392 vs. TAIR 10
Match: AT5G53130.1 (cyclic nucleotide gated channel 1 )

HSP 1 Score: 337.0 bits (863), Expect = 2.5e-92
Identity = 196/467 (41.97%), Postives = 284/467 (60.81%), Query Frame = 0

Query: 51  NGRLAMLSVIATSVDPLFFYILFVNEDRRCIGFHDEWKTAAVILRSVIDFLYAILTAYYF 110
           N    +  +IA S+DPLFFY+  +++ ++C+G   + +  A +LRS  D  Y +   + F
Sbjct: 96  NKIFVLACIIAVSLDPLFFYVPIIDDAKKCLGIDKKMEITASVLRSFTDVFYVLHIIFQF 155

Query: 111 HDGY--PLLNDETDGVLVR-----ARKFLLSSSRVDLLAFLPLPQLLALLVIPRSKGLHF 170
             G+  P       GVLV      A+++L S   +D+LA LPLPQ++ L++IP  +G   
Sbjct: 156 RTGFIAPSSRVFGRGVLVEDKREIAKRYLSSHFIIDILAVLPLPQMVILIIIPHMRGSSS 215

Query: 171 IHAIRALNYVVLVQYLSRVIRIYSFLMK-SGWSTILPQAAEARATFNLILYMLATHVVGA 230
           ++    L ++V  QY+ R IRIY    + +  S IL + A A A FNL LYMLA+HV GA
Sbjct: 216 LNTKNMLKFIVFFQYIPRFIRIYPLYKEVTRTSGILTETAWAGAAFNLFLYMLASHVFGA 275

Query: 231 FWYLSTIERKTMCWHGRCHY----C---PLNCNYVLIANNA--DDFCSEQAENDSKTYDF 290
           FWYL +IER+T+CW   C      C    L C+      NA  ++ C  Q  N +  +DF
Sbjct: 276 FWYLFSIERETVCWKQACERNNPPCISKLLYCDPETAGGNAFLNESCPIQTPN-TTLFDF 335

Query: 291 GVFKDALP--ILNSRDFIWKISYCFWWSLQKLSSFGQDLKTSGHLLEIYFAITITISGLV 350
           G+F DAL   ++ S+DF  K  YCFWW LQ LSS GQ+LKTS ++ EI FA+ I+I+GLV
Sbjct: 336 GIFLDALQSGVVESQDFPQKFFYCFWWGLQNLSSLGQNLKTSTYIWEICFAVFISIAGLV 395

Query: 351 LFALLIGNLQTYLQSTIRSKEKIRLKEQDIENCMTNYSIPDKLKTQVRKCKLYKQREAKG 410
           LF+ LIGN+QTYLQST    E++R+K +D E  M++  +P+ L+ ++R+ + YK +E +G
Sbjct: 396 LFSFLIGNMQTYLQSTTTRLEEMRVKRRDAEQWMSHRLLPENLRKRIRRYEQYKWQETRG 455

Query: 411 VGVEQLLQNLPNHISRDIKEHLCSTSLFNVLLKLDNMDKILSDAIYDLLKPKRYIEQTFI 470
           V  E LL NLP  + RDIK HLC   L  V +  + MD+ L DA+ D L+P  Y E+++I
Sbjct: 456 VDEENLLSNLPKDLRRDIKRHLCLALLMRVPM-FEKMDEQLLDALCDRLQPVLYTEESYI 515

Query: 471 VQEGGPVDEVVFIIQGKVLVSSKKDSKPEELNR--LTKGHFYGEELV 497
           V+EG PVDE++FI++GK+L  +    +   LN   L  G F GEEL+
Sbjct: 516 VREGDPVDEMLFIMRGKLLTITTNGGRTGFLNSEYLGAGDFCGEELL 560

BLAST of Spg027392 vs. TAIR 10
Match: AT4G01010.1 (cyclic nucleotide-gated channel 13 )

HSP 1 Score: 304.7 bits (779), Expect = 1.4e-82
Identity = 187/474 (39.45%), Postives = 273/474 (57.59%), Query Frame = 0

Query: 47  LHSKNGRLAMLSVIATSVDPLFFYILFVNEDRRCIGFHDEWKTAAVILRSVIDFLYAILT 106
           L + N      SVIA ++DPLFFYI  V+ +R C+  H   + AA +LR+ ID  Y I  
Sbjct: 76  LQNWNKIFLFASVIALAIDPLFFYIPIVDGERHCLNLHRNLEIAASVLRTFIDAFYIIHI 135

Query: 107 AYYFHDGY--PLLNDETDGVLVR-----ARKFLLSSSRVDLLAFLPLPQLLALLVIPRSK 166
            + F   Y  P       G LV      A K+L S   +DLL+ LPLPQL+ L VIP   
Sbjct: 136 VFQFRTAYISPSSRVFGRGELVDDPKAIAIKYLSSYFIIDLLSILPLPQLVVLAVIPNVN 195

Query: 167 GLHFIHAIRALNYVVLVQYLSRVIRIYSFLMK-SGWSTILPQAAEARATFNLILYMLATH 226
               +     L  V+  QY+ R++RIY    + +  S I+ + A A A +NL LYMLA+H
Sbjct: 196 KPVSLITKDYLITVIFTQYIPRILRIYPLYTEVTRTSGIVTETAWAGAAWNLSLYMLASH 255

Query: 227 VVGAFWYLSTIERKTMCWHGRCHYCPLNCNYVLI-----ANNADDFCSEQAE-------N 286
           V GA WYL ++ER+  CW   C   P  CN+  +     ++  +DF +            
Sbjct: 256 VFGALWYLISVEREDRCWREACEKIPEVCNFRFLYCDGNSSVRNDFLTTSCPFINPDDIT 315

Query: 287 DSKTYDFGVFKDALP--ILNSRDFIWKISYCFWWSLQKLSSFGQDLKTSGHLLEIYFAIT 346
           +S  ++FG+F DAL   I+ S DF  K  YCFWW L+ LS+ GQ+L TS  + EI FA++
Sbjct: 316 NSTVFNFGIFTDALKSGIVESDDFWKKFFYCFWWGLRNLSALGQNLNTSKFVGEIIFAVS 375

Query: 347 ITISGLVLFALLIGNLQTYLQSTIRSKEKIRLKEQDIENCMTNYSIPDKLKTQVRKCKLY 406
           I ISGLVLFALLIGN+Q YL+ST   +E++R++++D E  M++  +PD L+ ++R+ + Y
Sbjct: 376 ICISGLVLFALLIGNMQKYLESTTVREEEMRVRKRDAEQWMSHRMLPDDLRKRIRRYEQY 435

Query: 407 KQREAKGVGVEQLLQNLPNHISRDIKEHLCSTSLFNVLLKLDNMDKILSDAIYDLLKPKR 466
           K +E +GV  E LL+NLP  + RDIK H C   L  V L  + MD+ L DA+ D LKP  
Sbjct: 436 KWQETRGVEEENLLRNLPKDLRRDIKRHFCLDLLKKVPL-FEIMDEQLLDAVCDKLKPVL 495

Query: 467 YIEQTFIVQEGGPVDEVVFIIQGKVLVSSKKDSKPEELNR--LTKGHFYGEELV 497
           Y E ++ ++EG PV+E++F+++GK++ ++    +    N   L    F GE+L+
Sbjct: 496 YTENSYAIREGDPVEEMLFVMRGKLMSATTNGGRTGFFNAVYLKPSDFCGEDLL 548

BLAST of Spg027392 vs. TAIR 10
Match: AT2G46430.1 (cyclic nucleotide gated channel 3 )

HSP 1 Score: 301.6 bits (771), Expect = 1.2e-81
Identity = 186/486 (38.27%), Postives = 273/486 (56.17%), Query Frame = 0

Query: 36  LFRIIVGRKRKLHSKNGRLAMLSVIATSVDPLFFYILFVNEDRRCIGFHDEWKTAAVILR 95
           + R++      L S N    +LSV+A + DPLFFYI +V  +R C+    + +T A + R
Sbjct: 69  ILRVMNPNDSYLQSWNKIFLLLSVVALAFDPLFFYIPYVKPERFCLNLDKKLQTIACVFR 128

Query: 96  SVIDFLYAILTAYYFHDGYPL----------LNDETDGVLVRARKFLLSSSRVDLLAFLP 155
           + ID  Y +   + FH G+            LN++   + +R   +L S   +DLL+ LP
Sbjct: 129 TFIDAFYVVHMLFQFHTGFITPSSSGFGRGELNEKHKDIALR---YLGSYFLIDLLSILP 188

Query: 156 LPQLLALLVIPRSKGLHFIHAIRALNYVVLVQYLSRVIRIYS-FLMKSGWSTILPQAAEA 215
           +PQ++ L ++PR +    + A   L +V+  QY+ R+ RIY  F   +  S ++ + A A
Sbjct: 189 IPQVVVLAIVPRMRRPASLVAKELLKWVIFCQYVPRIARIYPLFKEVTRTSGLVTETAWA 248

Query: 216 RATFNLILYMLATHVVGAFWYLSTIERKTMCWHGRCH----------YC-PLNCNYVLIA 275
            A  NL LYMLA+HV G+FWYL +IERK  CW   C           YC P   +  L  
Sbjct: 249 GAALNLFLYMLASHVFGSFWYLISIERKDRCWREACAKIQNCTHAYLYCSPTGEDNRLFL 308

Query: 276 NNADDFCSEQAENDSKTYDFGVFKDALP--ILNSRDFIWKISYCFWWSLQKLSSFGQDLK 335
           N +      +   +S  ++FG+F DAL   ++ SRDF  K  YCFWW L+ LS+ GQ+LK
Sbjct: 309 NGSCPLIDPEEITNSTVFNFGIFADALQSGVVESRDFPKKFFYCFWWGLRNLSALGQNLK 368

Query: 336 TSGHLLEIYFAITITISGLVLFALLIGNLQTYLQSTIRSKEKIRLKEQDIENCMTNYSIP 395
           TS    EI FAI I ISGLVLFALLIGN+Q YLQST    E++R+K +D E  M++  +P
Sbjct: 369 TSAFEGEIIFAIVICISGLVLFALLIGNMQKYLQSTTVRVEEMRVKRRDAEQWMSHRMLP 428

Query: 396 DKLKTQVRKCKLYKQREAKGVGVEQLLQNLPNHISRDIKEHLCSTSLFNVLLKLDNMDKI 455
           D L+ ++RK + YK +E KGV  E LL +LP  + +DIK HLC   L   +     MD  
Sbjct: 429 DDLRKRIRKYEQYKWQETKGVEEEALLSSLPKDLRKDIKRHLC-LKLLKKVPWFQAMDDR 488

Query: 456 LSDAIYDLLKPKRYIEQTFIVQEGGPVDEVVFIIQGKVLVSSKKDSKPEELNR--LTKGH 496
           L DA+   LK   Y E+++IV+EG PV++++FI++G ++ ++    +    N   L  G 
Sbjct: 489 LLDALCARLKTVLYTEKSYIVREGEPVEDMLFIMRGNLISTTTYGGRTGFFNSVDLVAGD 548

BLAST of Spg027392 vs. TAIR 10
Match: AT2G46430.2 (cyclic nucleotide gated channel 3 )

HSP 1 Score: 301.6 bits (771), Expect = 1.2e-81
Identity = 186/486 (38.27%), Postives = 273/486 (56.17%), Query Frame = 0

Query: 36  LFRIIVGRKRKLHSKNGRLAMLSVIATSVDPLFFYILFVNEDRRCIGFHDEWKTAAVILR 95
           + R++      L S N    +LSV+A + DPLFFYI +V  +R C+    + +T A + R
Sbjct: 69  ILRVMNPNDSYLQSWNKIFLLLSVVALAFDPLFFYIPYVKPERFCLNLDKKLQTIACVFR 128

Query: 96  SVIDFLYAILTAYYFHDGYPL----------LNDETDGVLVRARKFLLSSSRVDLLAFLP 155
           + ID  Y +   + FH G+            LN++   + +R   +L S   +DLL+ LP
Sbjct: 129 TFIDAFYVVHMLFQFHTGFITPSSSGFGRGELNEKHKDIALR---YLGSYFLIDLLSILP 188

Query: 156 LPQLLALLVIPRSKGLHFIHAIRALNYVVLVQYLSRVIRIYS-FLMKSGWSTILPQAAEA 215
           +PQ++ L ++PR +    + A   L +V+  QY+ R+ RIY  F   +  S ++ + A A
Sbjct: 189 IPQVVVLAIVPRMRRPASLVAKELLKWVIFCQYVPRIARIYPLFKEVTRTSGLVTETAWA 248

Query: 216 RATFNLILYMLATHVVGAFWYLSTIERKTMCWHGRCH----------YC-PLNCNYVLIA 275
            A  NL LYMLA+HV G+FWYL +IERK  CW   C           YC P   +  L  
Sbjct: 249 GAALNLFLYMLASHVFGSFWYLISIERKDRCWREACAKIQNCTHAYLYCSPTGEDNRLFL 308

Query: 276 NNADDFCSEQAENDSKTYDFGVFKDALP--ILNSRDFIWKISYCFWWSLQKLSSFGQDLK 335
           N +      +   +S  ++FG+F DAL   ++ SRDF  K  YCFWW L+ LS+ GQ+LK
Sbjct: 309 NGSCPLIDPEEITNSTVFNFGIFADALQSGVVESRDFPKKFFYCFWWGLRNLSALGQNLK 368

Query: 336 TSGHLLEIYFAITITISGLVLFALLIGNLQTYLQSTIRSKEKIRLKEQDIENCMTNYSIP 395
           TS    EI FAI I ISGLVLFALLIGN+Q YLQST    E++R+K +D E  M++  +P
Sbjct: 369 TSAFEGEIIFAIVICISGLVLFALLIGNMQKYLQSTTVRVEEMRVKRRDAEQWMSHRMLP 428

Query: 396 DKLKTQVRKCKLYKQREAKGVGVEQLLQNLPNHISRDIKEHLCSTSLFNVLLKLDNMDKI 455
           D L+ ++RK + YK +E KGV  E LL +LP  + +DIK HLC   L   +     MD  
Sbjct: 429 DDLRKRIRKYEQYKWQETKGVEEEALLSSLPKDLRKDIKRHLC-LKLLKKVPWFQAMDDR 488

Query: 456 LSDAIYDLLKPKRYIEQTFIVQEGGPVDEVVFIIQGKVLVSSKKDSKPEELNR--LTKGH 496
           L DA+   LK   Y E+++IV+EG PV++++FI++G ++ ++    +    N   L  G 
Sbjct: 489 LLDALCARLKTVLYTEKSYIVREGEPVEDMLFIMRGNLISTTTYGGRTGFFNSVDLVAGD 548

BLAST of Spg027392 vs. TAIR 10
Match: AT1G01340.1 (cyclic nucleotide gated channel 10 )

HSP 1 Score: 290.0 bits (741), Expect = 3.5e-78
Identity = 174/464 (37.50%), Postives = 269/464 (57.97%), Query Frame = 0

Query: 59  VIATSVDPLFFYILFVNEDRRCIGFHDEWKTAAVILRSVIDFLYAILTAYYFHDGYPLLN 118
           V+A ++DPLFFYI  V+  R C+    + + AA +LR++ID  Y I   + F   Y   +
Sbjct: 83  VVALAIDPLFFYIPIVDSARHCLTLDSKLEIAASLLRTLIDAFYIIHIVFQFRTAYIAPS 142

Query: 119 DE-------TDGVLVRARKFLLSSSRVDLLAFLPLPQLLALLVIPRSKGLHFIHAIRALN 178
                     D     A K+L S   +DLL+ LPLPQ++ L VIP       +     L 
Sbjct: 143 SRVFGRGELVDDAKAIALKYLSSYFIIDLLSILPLPQIVVLAVIPSVNQPVSLLTKDYLK 202

Query: 179 YVVLVQYLSRVIRIYSFLMK-SGWSTILPQAAEARATFNLILYMLATHVVGAFWYLSTIE 238
           + ++ QY+ R++R+Y    + +  S I+ + A A A +NL LYMLA+HV GA WYL ++E
Sbjct: 203 FSIIAQYVPRILRMYPLYTEVTRTSGIVTETAWAGAAWNLSLYMLASHVFGALWYLISVE 262

Query: 239 RKTMCWHGRCH----------YCPLNCNYVLIANN----ADDFCSEQAENDSKTYDFGVF 298
           R+  CW   C           YC  + N   ++NN    +  F       +S  ++FG+F
Sbjct: 263 REDRCWQEACEKTKGCNMKFLYCENDRN---VSNNFLTTSCPFLDPGDITNSTIFNFGIF 322

Query: 299 KDALP--ILNSRDFIWKISYCFWWSLQKLSSFGQDLKTSGHLLEIYFAITITISGLVLFA 358
            DAL   ++ S DF  K  YCFWW L+ LS+ GQ+L+TS  + EI FAI+I ISGLVLFA
Sbjct: 323 TDALKSGVVESHDFWKKFFYCFWWGLRNLSALGQNLQTSKFVGEIIFAISICISGLVLFA 382

Query: 359 LLIGNLQTYLQSTIRSKEKIRLKEQDIENCMTNYSIPDKLKTQVRKCKLYKQREAKGVGV 418
           LLIGN+Q YL+ST   +E++R++++D E  M++  +P+ L+ ++R+ + Y+ +E +GV  
Sbjct: 383 LLIGNMQKYLESTTVREEEMRVRKRDAEQWMSHRMLPEDLRKRIRRYEQYRWQETRGVEE 442

Query: 419 EQLLQNLPNHISRDIKEHLCSTSLFNVLLKLDNMDKILSDAIYDLLKPKRYIEQTFIVQE 478
           E LL+NLP  + RDIK HLC   L  V L  + MD+ L DA+ D L+P  Y E +++++E
Sbjct: 443 ETLLRNLPKDLRRDIKRHLCLDLLKKVPL-FEIMDEQLLDAVCDRLRPVLYTENSYVIRE 502

Query: 479 GGPVDEVVFIIQGKVLVSSKKDSKPEELN--RLTKGHFYGEELV 497
           G PV E++F+++G+++ ++    +    N   L    F GE+L+
Sbjct: 503 GDPVGEMLFVMRGRLVSATTNGGRSGFFNAVNLKASDFCGEDLL 542

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022141010.16.8e-14058.26cyclic nucleotide-gated ion channel 1-like [Momordica charantia][more]
XP_038904375.11.2e-13958.35cyclic nucleotide-gated ion channel 1-like [Benincasa hispida] >XP_038904379.1 c... [more]
XP_022968617.17.5e-13958.23cyclic nucleotide-gated ion channel 1-like isoform X2 [Cucurbita maxima][more]
XP_022968616.17.5e-13958.23cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita maxima][more]
XP_023541623.11.3e-13858.01cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita pepo subsp. pep... [more]
Match NameE-valueIdentityDescription
O657173.5e-9141.97Cyclic nucleotide-gated ion channel 1 OS=Arabidopsis thaliana OX=3702 GN=CNGC1 P... [more]
Q9LD401.9e-8139.45Putative cyclic nucleotide-gated ion channel 13 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9SKD71.6e-8038.27Probable cyclic nucleotide-gated ion channel 3 OS=Arabidopsis thaliana OX=3702 G... [more]
Q9LNJ04.9e-7737.50Probable cyclic nucleotide-gated ion channel 10 OS=Arabidopsis thaliana OX=3702 ... [more]
O822263.2e-7637.39Probable cyclic nucleotide-gated ion channel 6 OS=Arabidopsis thaliana OX=3702 G... [more]
Match NameE-valueIdentityDescription
A0A6J1CGR83.3e-14058.26cyclic nucleotide-gated ion channel 1-like OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A6J1HU013.6e-13958.23cyclic nucleotide-gated ion channel 1-like isoform X2 OS=Cucurbita maxima OX=366... [more]
A0A6J1HVE33.6e-13958.23cyclic nucleotide-gated ion channel 1-like isoform X1 OS=Cucurbita maxima OX=366... [more]
A0A6J1G2D21.8e-13858.23cyclic nucleotide-gated ion channel 1-like isoform X1 OS=Cucurbita moschata OX=3... [more]
A0A6J1G2951.8e-13858.23cyclic nucleotide-gated ion channel 1-like isoform X2 OS=Cucurbita moschata OX=3... [more]
Match NameE-valueIdentityDescription
AT5G53130.12.5e-9241.97cyclic nucleotide gated channel 1 [more]
AT4G01010.11.4e-8239.45cyclic nucleotide-gated channel 13 [more]
AT2G46430.11.2e-8138.27cyclic nucleotide gated channel 3 [more]
AT2G46430.21.2e-8138.27cyclic nucleotide gated channel 3 [more]
AT1G01340.13.5e-7837.50cyclic nucleotide gated channel 10 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005821Ion transport domainPFAMPF00520Ion_transcoord: 57..349
e-value: 1.1E-17
score: 64.1
NoneNo IPR availableGENE3D1.10.287.630Helix hairpin bincoord: 349..414
e-value: 7.3E-6
score: 28.2
NoneNo IPR availableGENE3D1.10.287.70coord: 53..347
e-value: 8.4E-11
score: 43.7
NoneNo IPR availablePANTHERPTHR45651:SF36CYCLIC NUCLEOTIDE-GATED CHANNEL 21coord: 35..497
NoneNo IPR availablePANTHERPTHR45651CYCLIC NUCLEOTIDE-GATED ION CHANNEL 15-RELATED-RELATEDcoord: 35..497
NoneNo IPR availableSUPERFAMILY81324Voltage-gated potassium channelscoord: 57..345
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 424..497
e-value: 1.5E-14
score: 55.8
IPR000595Cyclic nucleotide-binding domainPROSITEPS50042CNMP_BINDING_3coord: 424..497
score: 12.9694
IPR000595Cyclic nucleotide-binding domainCDDcd00038CAP_EDcoord: 425..496
e-value: 2.05321E-6
score: 44.6242
IPR018490Cyclic nucleotide-binding-likeSUPERFAMILY51206cAMP-binding domain-likecoord: 350..494

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg027392.1Spg027392.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006811 ion transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016020 membrane
molecular_function GO:0005216 ion channel activity