Spg027263 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg027263
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionReverse transcriptase
Locationscaffold8: 2629688 .. 2631423 (+)
RNA-Seq ExpressionSpg027263
SyntenySpg027263
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAAGAAAGGAAAGAAAAGAAAAGGAAAAGGAAAAGGAAAATGGAAGGAAAAGAAAAAAAAAAGAGAGAAAAGAAATCAGCAAAGAAAAAAAAAAAGAGAAAAGAAAGGAAGTGGAAATGGACAGCACAAAGCCCACCACCGACAACCACATTTTTCAGCCCAATTTCATTTCATTTCTCCATCATTTTCCATTCAAACTTTCAGAGCACTTTGAGGAGAGAGTGAAGGAAGAAGAAGAAGAAGAATTTCGTGGTTTGAGGTTGAAGAAGATAGGAAAAAGTTCATGTAAAGCCAGCCATTGCAGAATCTGGGTGCAAACTGTCAACCTCTGGTTTTTTATTCGGTTTGAGCTACACAGAAGGAATCAAGAGGTGAGGTTTGATTTTCTTGAAATTTAGTTCATTTTCTAACAAATTTCATGAAGGAAGCTTGACCTAAATCGTAGAGAAGTGGATGGTTTTTGGAATGGAAAATAGAGCACAGGATTTTCGAGCAAAGCAGGAGGATCTCTGGTTTTCTCTGATGTTTTGAGCATTCTGGGGTGTACAAGTTGAATCAGAAGCTCTATTTGGTATGGTTGGAAAGCTTATTCAGTTATCTACAACTTTCATGAAGAATTCGCGAGCCAAAAATGACTTTACGTAGATTTAATTTCAGGAGGAAGATAAAGGGTAACAGAGAAGCTCGAATCTGTGTTGTCTGACTTCGGGGCATTTCTGGACAAATTGGTTGGGAATCTCGTTTTCAGTTTTTCAGGTACGTTGTAGAGCGTTCGAAAATGAAGAGTTTGATGTGTATTTCATTAATTTTGGTTTAGTATTGAGGAAGTTATGGGTATTGGAAGCTAGGGTATAGAATCTGGAAAATCTGGAAAGTAGAATTGTGAATGTGATTCTTAATCCAATTGTTTAAAAATTCATGAGCATGGAAAGACAAAGCTATCTATGGTTCTAATGCTCGGTTTTGGTTATTTGACAGGCCACGAGGAAATAGGTCGAGTCTACAAACCGGAGAACTAGAAAAGACAGTGAGTGACAAAATGAATTTCGAAAATGTTCTTAAACGGTTGTTCATGATTATATGTTTTATATGCTTTACCATGAATGATATATGATTTTTATGAACGGTTTCAAGCATGAGTTTTGAGCTCAGATTTTCTAATGAGGTTTATATACGAGCACGTGTCTAATGACTTTATGAAAAATAGCTGAAACGATATCTTTTAAAGTAAAGTATGGATTAGCAAATACTGTCGTGAGTGTACTCCGTGACAACGATGCTGTCGTGAGTGCTGGGCGGGCCCCACTACGACAAAGACGATGGAAGTGCTGGGCGGGCCCCACTACATCGTAGGACTAGTAAACGTTGGTTGTACTGGGCGTGCCCTACACAACGTAAATCGGTCATGTTAGTTAAAACGTTTGATGTACTTAATATGCCTTGCTAGATTTTTAATGACGAACTTGCGAGATGTTTTGGAAAGCCTTTATTATTACACGTTTATGTTAAACGCTTTGATTGAGCATATTTATACGTTTAAAGGTTTATCAAGTGTTTTGACTTCCGGATTTAGAGTTTTGAAGTTAGTCACTCACTGGGCTTCGTAGCTCATCCTTTCAAAATGTTTTTCCACTTTCAGGTAGAGATCGAGCTCCCGGTGCCTGATACACTGCCAACGTCTGTTGAAAGTTTTAGATCAAGCTCCAGTACGTGGTTGGAGTTGTATATTGAGTCC

mRNA sequence

ATGGACAGCACAAAGCCCACCACCGACAACCACATTTTTCAGCCCAATTTCATTTCATTTCTCCATCATTTTCCATTCAAACTTTCAGAGCACTTTGAGGAGAGAGTGAAGGAAGAAGAAGAAGAAGAATTTCGTGGTTTGAGGTTGAAGAAGATAGGAAAAAGTTCATGTAAAGCCAGCCATTGCAGAATCTGGGTGCAAACTGTCAACCTCTGGTTTTTTATTCGGTTTGAGCTACACAGAAGGAATCAAGAGCATTCTGGGGTGTACAAGTTGAATCAGAAGCTCTATTTGTTTTTCAGGCCACGAGGAAATAGGTCGAGTCTACAAACCGGAGAACTAGAAAAGACAGTAGAGATCGAGCTCCCGGTGCCTGATACACTGCCAACGTCTGTTGAAAGTTTTAGATCAAGCTCCAGTACGTGGTTGGAGTTGTATATTGAGTCC

Coding sequence (CDS)

ATGGACAGCACAAAGCCCACCACCGACAACCACATTTTTCAGCCCAATTTCATTTCATTTCTCCATCATTTTCCATTCAAACTTTCAGAGCACTTTGAGGAGAGAGTGAAGGAAGAAGAAGAAGAAGAATTTCGTGGTTTGAGGTTGAAGAAGATAGGAAAAAGTTCATGTAAAGCCAGCCATTGCAGAATCTGGGTGCAAACTGTCAACCTCTGGTTTTTTATTCGGTTTGAGCTACACAGAAGGAATCAAGAGCATTCTGGGGTGTACAAGTTGAATCAGAAGCTCTATTTGTTTTTCAGGCCACGAGGAAATAGGTCGAGTCTACAAACCGGAGAACTAGAAAAGACAGTAGAGATCGAGCTCCCGGTGCCTGATACACTGCCAACGTCTGTTGAAAGTTTTAGATCAAGCTCCAGTACGTGGTTGGAGTTGTATATTGAGTCC

Protein sequence

MDSTKPTTDNHIFQPNFISFLHHFPFKLSEHFEERVKEEEEEEFRGLRLKKIGKSSCKASHCRIWVQTVNLWFFIRFELHRRNQEHSGVYKLNQKLYLFFRPRGNRSSLQTGELEKTVEIELPVPDTLPTSVESFRSSSSTWLELYIES
Homology
[Close]
BLAST of Spg027263 vs. NCBI nr
Match: KAA0053276.1 (uncharacterized protein E6C27_scaffold102G00750 [Cucumis melo var. makuwa])

HSP 1 Score: 130.6 bits (327), Expect = 1.1e-26
Identity = 85/174 (48.85%), Postives = 98/174 (56.32%), Query Frame = 0 

Query: 4   TKPTTDNHIFQPNFISFLHHFPFKLSEHFEERVKEEEEEEFRGLRLKKIGKSSCKASHCR 63
           TKPTTD  IFQ NFI FLHHF  KLSE+F+ERVK EEEEEFRGLRLKK+ K   ++   +
Sbjct: 145 TKPTTDKLIFQSNFILFLHHFSSKLSEYFKERVK-EEEEEFRGLRLKKM-KKFMQSQTLQ 204

Query: 64  IWVQTVNLWFFIRFELHRRNQEHSGVYKLNQKLYLFFRPRGNRSSLQTGELEKT------ 123
               T+NLWF +    +                    RPRGNR SLQTG+L+KT      
Sbjct: 205 NLGATINLWFVLIGLSYPEGT---------------MRPRGNRPSLQTGKLDKTVIFQLS 264

Query: 124 ----------------------VEIELPVPDTLPTSVESFRSSSSTWLELYIES 150
                                 VEIELP+PDTLPT  ESF S+SSTWLELY ES
Sbjct: 265 TLSVLRDNDAVLILFKMFPTFQVEIELPMPDTLPTFAESFESNSSTWLELYFES 301

[Close]
BLAST of Spg027263 vs. NCBI nr
Match: TYK08801.1 (hypothetical protein E5676_scaffold796G00040 [Cucumis melo var. makuwa])

HSP 1 Score: 123.6 bits (309), Expect = 1.4e-24
Identity = 94/229 (41.05%), Postives = 104/229 (45.41%), Query Frame = 0 

Query: 12  IFQPNFISFLHHFPFKLSEHFEERVKEEEEEEFRGLRLKKIGKSSCKASHCRIWVQTVNL 71
           +FQPNFISFLHHFP KLSEHFEERVK EEEEEFRGLRLKKIGKSSCKA+HCRIW    + 
Sbjct: 56  LFQPNFISFLHHFPSKLSEHFEERVK-EEEEEFRGLRLKKIGKSSCKANHCRIW----ST 115

Query: 72  WFFIR---FELHRRNQEHSGVYKLNQKLYLFFR----------PRGNRSSLQTGELEK-- 131
            F  +   F +         V K NQKLY              PRGNRSSLQTG+L+K  
Sbjct: 116 GFSSKPRGFLVFSDALSILEVQKSNQKLYWDIESGKSGKWDVMPRGNRSSLQTGQLDKIE 175

Query: 132 ------------------------------------------------------------ 149
                                                                       
Sbjct: 176 ISIDYMTEILSLRLYGTVCPQVIFPLSTLSVLRDNDAVVSVGQTHYDKDDGVPGGPHYIV 235

[Close]
BLAST of Spg027263 vs. NCBI nr
Match: TYK04298.1 (pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 107.1 bits (266), Expect = 1.4e-19
Identity = 80/163 (49.08%), Postives = 91/163 (55.83%), Query Frame = 0 

Query: 4   TKPTTDNHIFQPNFISFLHHFPFKLSEHFEERVKEEEEEEFRGLRLKKIGKSSCKASHCR 63
           TKPTT   IFQ NFISFLHHFP KL EHFEERVK EEEEEFR ++ +   +S+  +S   
Sbjct: 15  TKPTTYKLIFQRNFISFLHHFPSKLLEHFEERVK-EEEEEFRAIQKELRDESTGFSS--- 74

Query: 64  IWVQTVNLWFFIRFELHRRNQEHSGVYKLNQKLYLFFR----------PRGNRSSLQTGE 123
                     F+ F      QE   V+K NQKLYL             PRGNRSSLQTGE
Sbjct: 75  ------KPGGFLVFSDALSIQE---VHKSNQKLYLDIESGKSGKWDVMPRGNRSSLQTGE 134

Query: 124 LEKT-----------------VEIELPVPDTLPTSVESFRSSS 140
           L+KT                 VEIELPVPD LPTS +S  S+S
Sbjct: 135 LDKTVIFLLSTLSVLRDNDAVVEIELPVPDRLPTSAQSSGSNS 164

[Close]
BLAST of Spg027263 vs. NCBI nr
Match: KAA0040575.1 (putative integrase [Cucumis melo var. makuwa] >TYK05618.1 putative integrase [Cucumis melo var. makuwa])

HSP 1 Score: 88.2 bits (217), Expect = 6.5e-14
Identity = 65/134 (48.51%), Postives = 70/134 (52.24%), Query Frame = 0 

Query: 30  EHFEERVKEEEEEEFRGLRLKKIGKSSCKASHCRIWVQTVNLWFFIRFELHRRNQEHSGV 89
           +HFEERVK EEEEEFRGLRLKKIGKSSC ASHCRIWVQ      F+ F       E   V
Sbjct: 53  KHFEERVK-EEEEEFRGLRLKKIGKSSCNASHCRIWVQLSTSGGFLVFSDALSILE---V 112

Query: 90  YKLNQKLYLF--------------------FRPRGNRSSLQTGELEKT------------ 127
           +K NQKL L                     +  RGNR SLQ GEL+KT            
Sbjct: 113 HKSNQKLCLMNWLRISFSFLLDIESGKSGKWDLRGNRPSLQIGELDKTVIFPLSTFCVLR 172

[Close]
BLAST of Spg027263 vs. NCBI nr
Match: KAA0040420.1 (NBS-LRR type resistance protein [Cucumis melo var. makuwa])

HSP 1 Score: 84.0 bits (206), Expect = 1.2e-12
Identity = 49/82 (59.76%), Postives = 54/82 (65.85%), Query Frame = 0 

Query: 68  TVNLWFFIRFELHRRNQEHSGVYKLNQKLYLFFRPRGNRSSLQTGELEKTVEIELPVPDT 127
           TVN W F+ F L          Y    K     RPRGNR SLQTG+L+KTVEIELP+PDT
Sbjct: 12  TVNFW-FVLFSLS---------YPEGTK-----RPRGNRPSLQTGKLDKTVEIELPMPDT 71

Query: 128 LPTSVESFRSSSSTWLELYIES 150
           LPT  ESF S+SSTWLELY ES
Sbjct: 72  LPTFAESFGSNSSTWLELYFES 78

[Close]
BLAST of Spg027263 vs. ExPASy TrEMBL
Match: A0A5A7UGD0 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold102G00750 PE=4 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 5.6e-27
Identity = 85/174 (48.85%), Postives = 98/174 (56.32%), Query Frame = 0 

Query: 4   TKPTTDNHIFQPNFISFLHHFPFKLSEHFEERVKEEEEEEFRGLRLKKIGKSSCKASHCR 63
           TKPTTD  IFQ NFI FLHHF  KLSE+F+ERVK EEEEEFRGLRLKK+ K   ++   +
Sbjct: 145 TKPTTDKLIFQSNFILFLHHFSSKLSEYFKERVK-EEEEEFRGLRLKKM-KKFMQSQTLQ 204

Query: 64  IWVQTVNLWFFIRFELHRRNQEHSGVYKLNQKLYLFFRPRGNRSSLQTGELEKT------ 123
               T+NLWF +    +                    RPRGNR SLQTG+L+KT      
Sbjct: 205 NLGATINLWFVLIGLSYPEGT---------------MRPRGNRPSLQTGKLDKTVIFQLS 264

Query: 124 ----------------------VEIELPVPDTLPTSVESFRSSSSTWLELYIES 150
                                 VEIELP+PDTLPT  ESF S+SSTWLELY ES
Sbjct: 265 TLSVLRDNDAVLILFKMFPTFQVEIELPMPDTLPTFAESFESNSSTWLELYFES 301

[Close]
BLAST of Spg027263 vs. ExPASy TrEMBL
Match: A0A5D3CAC6 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold796G00040 PE=4 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 6.8e-25
Identity = 94/229 (41.05%), Postives = 104/229 (45.41%), Query Frame = 0 

Query: 12  IFQPNFISFLHHFPFKLSEHFEERVKEEEEEEFRGLRLKKIGKSSCKASHCRIWVQTVNL 71
           +FQPNFISFLHHFP KLSEHFEERVK EEEEEFRGLRLKKIGKSSCKA+HCRIW    + 
Sbjct: 56  LFQPNFISFLHHFPSKLSEHFEERVK-EEEEEFRGLRLKKIGKSSCKANHCRIW----ST 115

Query: 72  WFFIR---FELHRRNQEHSGVYKLNQKLYLFFR----------PRGNRSSLQTGELEK-- 131
            F  +   F +         V K NQKLY              PRGNRSSLQTG+L+K  
Sbjct: 116 GFSSKPRGFLVFSDALSILEVQKSNQKLYWDIESGKSGKWDVMPRGNRSSLQTGQLDKIE 175

Query: 132 ------------------------------------------------------------ 149
                                                                       
Sbjct: 176 ISIDYMTEILSLRLYGTVCPQVIFPLSTLSVLRDNDAVVSVGQTHYDKDDGVPGGPHYIV 235

[Close]
BLAST of Spg027263 vs. ExPASy TrEMBL
Match: A0A5D3BZ50 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold527G00270 PE=4 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 6.6e-20
Identity = 80/163 (49.08%), Postives = 91/163 (55.83%), Query Frame = 0 

Query: 4   TKPTTDNHIFQPNFISFLHHFPFKLSEHFEERVKEEEEEEFRGLRLKKIGKSSCKASHCR 63
           TKPTT   IFQ NFISFLHHFP KL EHFEERVK EEEEEFR ++ +   +S+  +S   
Sbjct: 15  TKPTTYKLIFQRNFISFLHHFPSKLLEHFEERVK-EEEEEFRAIQKELRDESTGFSS--- 74

Query: 64  IWVQTVNLWFFIRFELHRRNQEHSGVYKLNQKLYLFFR----------PRGNRSSLQTGE 123
                     F+ F      QE   V+K NQKLYL             PRGNRSSLQTGE
Sbjct: 75  ------KPGGFLVFSDALSIQE---VHKSNQKLYLDIESGKSGKWDVMPRGNRSSLQTGE 134

Query: 124 LEKT-----------------VEIELPVPDTLPTSVESFRSSS 140
           L+KT                 VEIELPVPD LPTS +S  S+S
Sbjct: 135 LDKTVIFLLSTLSVLRDNDAVVEIELPVPDRLPTSAQSSGSNS 164

[Close]
BLAST of Spg027263 vs. ExPASy TrEMBL
Match: A0A5A7TGM2 (Putative integrase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G00750 PE=4 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 3.2e-14
Identity = 65/134 (48.51%), Postives = 70/134 (52.24%), Query Frame = 0 

Query: 30  EHFEERVKEEEEEEFRGLRLKKIGKSSCKASHCRIWVQTVNLWFFIRFELHRRNQEHSGV 89
           +HFEERVK EEEEEFRGLRLKKIGKSSC ASHCRIWVQ      F+ F       E   V
Sbjct: 53  KHFEERVK-EEEEEFRGLRLKKIGKSSCNASHCRIWVQLSTSGGFLVFSDALSILE---V 112

Query: 90  YKLNQKLYLF--------------------FRPRGNRSSLQTGELEKT------------ 127
           +K NQKL L                     +  RGNR SLQ GEL+KT            
Sbjct: 113 HKSNQKLCLMNWLRISFSFLLDIESGKSGKWDLRGNRPSLQIGELDKTVIFPLSTFCVLR 172

[Close]
BLAST of Spg027263 vs. ExPASy TrEMBL
Match: A0A5A7TAS6 (NBS-LRR type resistance protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold35G00450 PE=4 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 6.0e-13
Identity = 49/82 (59.76%), Postives = 54/82 (65.85%), Query Frame = 0 

Query: 68  TVNLWFFIRFELHRRNQEHSGVYKLNQKLYLFFRPRGNRSSLQTGELEKTVEIELPVPDT 127
           TVN W F+ F L          Y    K     RPRGNR SLQTG+L+KTVEIELP+PDT
Sbjct: 12  TVNFW-FVLFSLS---------YPEGTK-----RPRGNRPSLQTGKLDKTVEIELPMPDT 71

Query: 128 LPTSVESFRSSSSTWLELYIES 150
           LPT  ESF S+SSTWLELY ES
Sbjct: 72  LPTFAESFGSNSSTWLELYFES 78

The following BLAST results are available for this feature:
BLAST of Spg027263 vs. NCBI nr
Match NameE-valueIdentityDescription
KAA0053276.11.1e-2648.85uncharacterized protein E6C27_scaffold102G00750 [Cucumis melo var. makuwa][more]
TYK08801.11.4e-2441.05hypothetical protein E5676_scaffold796G00040 [Cucumis melo var. makuwa][more]
TYK04298.11.4e-1949.08pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa][more]
KAA0040575.16.5e-1448.51putative integrase [Cucumis melo var. makuwa] >TYK05618.1 putative integrase [Cu... [more]
KAA0040420.11.2e-1259.76NBS-LRR type resistance protein [Cucumis melo var. makuwa][more]
BLAST of Spg027263 vs. NCBI nr
Match NameE-valueIdentityDescription
BLAST of Spg027263 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5A7UGD05.6e-2748.85Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A5D3CAC66.8e-2541.05Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A5D3BZ506.6e-2049.08Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946... [more]
A0A5A7TGM23.2e-1448.51Putative integrase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G00... [more]
A0A5A7TAS66.0e-1359.76NBS-LRR type resistance protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
BLAST of Spg027263 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg027263.1Spg027263.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043170 macromolecule metabolic process
biological_process GO:0006807 nitrogen compound metabolic process
biological_process GO:0044238 primary metabolic process
molecular_function GO:0003824 catalytic activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008270 zinc ion binding