Spg026448 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg026448
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionRNase H domain-containing protein
Locationscaffold13: 15095403 .. 15097905 (+)
RNA-Seq ExpressionSpg026448
SyntenySpg026448
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGGATCAAAAGTAATGAGGATTCTTCTTCTTTGGACAGAAATCGACGGTGGTCTCCTCCGAATGGCGTATTTTGGAAGCTTAACTCTGATGCTGCATGTTCGAAAGAGGTCTCGATGACAAGTCTTGGGATGATCTGCAGAAATTTTGAGGGCAAAGTTACGGTAGCTGCATCAAAGGCTTTGGACGTGAGGTTGGAGGCCCATATTGCTGAATTATGGGCCATCCTTGAAGGTTTAAAGCTGGTTATTTCATTGGGCTGCACTATGTTAGTTGTGGAGTCCGATTGTCAACTTGCTATCAATTTCATCAATAAGGTTTCTCTTATTTGGTCAGATTCAGAGGCCATAGTTCTGTCTATTTGGAGCCTCTTACCTTTTTTTTAGGGATATCTCTTTCAATTTTGCTCCTAGATCTTGTAATAGGGTTGCCCACGAGATTGCTCGATATGGGAAATCTGTAAATAGCCATGACGTTTGGGTCTCTAATTTCTCAAACTGGATGTGTATGTTTCCCTAATTAATTAAGCTAGTCTTTTATATTTAATTGGCCAAAATTATGCATTTTATGTTCTTTTTGTAGGAATCATGTTTCGGAGTTAAATCGAGAAATTGGAGCTAAATACGTGTTCGGGACATGAAAATTGGCTAAAGAACGAAAAAGATTCAACCAAGAAGAAGTCAAAAGAGAGCCATCCGTAGGCCAGCGTCGGGACGCTGCTCTTGGAGCGTCTCGACGCTGCCTTTCCTTTTCTGAATTGGGCGGCAACAGAGACAGCGTCGAGACGCTATCTCCTTAGCGTCGAGACGCTGTGACAGCGTCGCGACACTATGTACACAGCGTCGCGACGCTACCCGTAGATTTTCTATAAATTCCTCTTTTCAGCTACGGATTTGGGAGGCCAATTGGTTAGATTTTAGCCCTAATTTCGACTAGAGACCTTTTGTGGGAGCTAAAGGCAAGGCCAAGGAGCGGGAATCAAGCGAGAAGACGTGCAGATCGTGACGGGGACGTGCTGGTTTCGACGGATTCGAGTTTTCCCTTTTCTTTCCCTTAACTCTTGTAGTTTTTATGGCTTTTCTTTCAATTAGTGTTTATGGTTTAGAGACTATGAATTGCTAGTTGTTTGTCTTAGGGATTGATGTAATTGTTTAAGTTTGTTGGATTAATTTTTGGTATGAATGTGCTTTTATTCTATGCATGCAATTTTGTGTTTAATGCTTTCAATTACTTGATCACTAATTGAATGATTTAAGTGTTGAATCTAACTCGGGAGAGAGGATTTGGCCTAAGACTTACCTTGTTTAATTGCATGACTAGAGTAGAAATTCTAAGTTTGTGACAGGATTTGGAATATCTTTAGAAGCATAACAAGGACCGAACCGAATAATGCCTTGCATGTTAATGAACTTAGATAGGGATATCAAGTTGGTTTATATGCATGAGAATCAATTATACTCGGGAGAGGTAATCGTGATAAAAGTTTCCTTGGCTTGTAGAATAATTGCACGGTCAATTCATTCCTATTCATCAATCCTCATTCTTAATCAATGAAATCAATTCCCCAAGAATCTTTCCATTTGAGTCTCCACTTTTATTTTATGCTTTTTGTCTTTAGATTTATCTTTTATCATTGAGTTTTGCTCCCTAAATAAAATTGAACAAAAGTATTCACGATACTTAATTTGCTAGAAAACCAATCCAGAGGACGACACTCGTTTTACACGAAAACACTATATTATTACTTGACTCGTACACTTGCGGTAAGCAATTTGCACATCAATAATTTTGGCCAATTAAATATAAAAGACTAGCTTAATTAATTAGGGAAACATAGCTAGCTATGCTTGCTATCAGTAGGACATAAGACCTTGGTTATAAGGGAACTCTACCGACAAATGGTTCGGAACCGGAGTTGAATTAATCATAGTCTTACTAGCACGCAATGTCACTAGGTTATTAAAAGTGTTTAATCACCTAGAAATTTAGTATAAAGACCTTGTTTAATAAGGCATAGACTTAGATAGATTCATTTAGCTGGAATTTACCTAAGTAATAAGACCCTAAAACTTTACGATACTTTAGTGGGAGGAAAAAGTATAGAAACGAAATATTCATATTAAGTTTCTACTCTCACGTCCCCCTGATTTCACACCTTGAGATCCATGCTTGGCCTCGAGGCGCCCTGGGAGCGTCCCCCTACGGATGGTGTTTGCATGGGTTAATATCAAAGTGAATGGGGAAAGTGTTCATAGTAAGTGGGAGAAGGAAACGTGTCAAACGTATCCTGTGGTCTCCGTCATTAGGTTGCACCGTGAGATTCCTATGCGCTGCATGCGAGTCGCCCTGGGAGCGATCACCCTACGGAGGGCTTGCGCATGGGATTCAGAACAACGCAAACTCCAGAAAAGGATATGGTTTCCTAGGGCCATTCCCAACATTAGCTCTTCCCTACGGTGGCATTGTTGGGGCCGCCCTCTGCGATCTGAAAATGATGGGTAA

mRNA sequence

ATGCGGATCAAAAGTAATGAGGATTCTTCTTCTTTGGACAGAAATCGACGGTGGTCTCCTCCGAATGGCGTATTTTGGAAGCTTAACTCTGATGCTGCATGTTCGAAAGAGGTCTCGATGACAAGTCTTGGGATGATCTGCAGAAATTTTGAGGGCAAAGTTACGGTAGCTGCATCAAAGGCTTTGGACGTGAGGTTGGAGGCCCATATTGCTGAATTATGGGCCATCCTTGAAGGTTTAAAGCTGGTTATTTCATTGGGCTGCACTATGTTAGTTGTGGAGTCCGATTGTCAACTTGCTATCAATTTCATCAATAAGAGACAGCGTCGAGACGCTATCTCCTTAGCGTCGAGACGCTGTGACAGCGTCGCGACACTATGTACACAGCGTCGCGACGCTACCCGTAGATTTTCTATAAATTCCTCTTTTCAGCTACGGATTTGGGAGGCCAATTGGCGCCCTGGGAGCGTCCCCCTACGGATGGTGTTTGCATGGGTTAATATCAAAGTGAATGGGGAAAGTGTTCATAGTAAGTGGGAGAAGGAAACGTGTCAAACGTATCCTGTGGTCTCCGTCATTAGGTTGCACCGTGAGATTCCTATGCGCTGCATGCGAGTCGCCCTGGGAGCGATCACCCTACGGAGGGCTTGCGCATGGGATTCAGAACAACGCAAACTCCAGAAAAGGATATGGTTTCCTAGGGCCATTCCCAACATTAGCTCTTCCCTACGGTGGCATTGTTGGGGCCGCCCTCTGCGATCTGAAAATGATGGGTAA

Coding sequence (CDS)

ATGCGGATCAAAAGTAATGAGGATTCTTCTTCTTTGGACAGAAATCGACGGTGGTCTCCTCCGAATGGCGTATTTTGGAAGCTTAACTCTGATGCTGCATGTTCGAAAGAGGTCTCGATGACAAGTCTTGGGATGATCTGCAGAAATTTTGAGGGCAAAGTTACGGTAGCTGCATCAAAGGCTTTGGACGTGAGGTTGGAGGCCCATATTGCTGAATTATGGGCCATCCTTGAAGGTTTAAAGCTGGTTATTTCATTGGGCTGCACTATGTTAGTTGTGGAGTCCGATTGTCAACTTGCTATCAATTTCATCAATAAGAGACAGCGTCGAGACGCTATCTCCTTAGCGTCGAGACGCTGTGACAGCGTCGCGACACTATGTACACAGCGTCGCGACGCTACCCGTAGATTTTCTATAAATTCCTCTTTTCAGCTACGGATTTGGGAGGCCAATTGGCGCCCTGGGAGCGTCCCCCTACGGATGGTGTTTGCATGGGTTAATATCAAAGTGAATGGGGAAAGTGTTCATAGTAAGTGGGAGAAGGAAACGTGTCAAACGTATCCTGTGGTCTCCGTCATTAGGTTGCACCGTGAGATTCCTATGCGCTGCATGCGAGTCGCCCTGGGAGCGATCACCCTACGGAGGGCTTGCGCATGGGATTCAGAACAACGCAAACTCCAGAAAAGGATATGGTTTCCTAGGGCCATTCCCAACATTAGCTCTTCCCTACGGTGGCATTGTTGGGGCCGCCCTCTGCGATCTGAAAATGATGGGTAA

Protein sequence

MRIKSNEDSSSLDRNRRWSPPNGVFWKLNSDAACSKEVSMTSLGMICRNFEGKVTVAASKALDVRLEAHIAELWAILEGLKLVISLGCTMLVVESDCQLAINFINKRQRRDAISLASRRCDSVATLCTQRRDATRRFSINSSFQLRIWEANWRPGSVPLRMVFAWVNIKVNGESVHSKWEKETCQTYPVVSVIRLHREIPMRCMRVALGAITLRRACAWDSEQRKLQKRIWFPRAIPNISSSLRWHCWGRPLRSENDG
Homology
BLAST of Spg026448 vs. NCBI nr
Match: XP_040996092.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC121242268 [Juglans microcarpa x Juglans regia])

HSP 1 Score: 76.6 bits (187), Expect = 3.4e-10
Identity = 41/90 (45.56%), Postives = 56/90 (62.22%), Query Frame = 0

Query: 17  RWSPPNGVFWKLNSDAACSKEVSMTSLGMICRNFEGKVTVAASKALDVRLEAHIAELWAI 76
           RWSPP+    KLN D A   E++ TSLG I RN  G V +A S+A  V+LE    E  A+
Sbjct: 317 RWSPPSQDSLKLNIDGALFFELNKTSLGAILRNHVGDVLMAVSRAKSVQLELEQVEAAAL 376

Query: 77  LEGLKLVISLGCTMLVVESDCQLAINFINK 107
           L GL+L +++G + LV+ESDC   +  +NK
Sbjct: 377 LRGLQLCLNIGISKLVIESDCLFLVEEVNK 406

BLAST of Spg026448 vs. NCBI nr
Match: OMO72757.1 (hypothetical protein CCACVL1_17615 [Corchorus capsularis])

HSP 1 Score: 70.9 bits (172), Expect = 1.9e-08
Identity = 47/123 (38.21%), Postives = 64/123 (52.03%), Query Frame = 0

Query: 17   RWSPPNGVFWKLNSDAACSKEVSMTSLGMICRNFEGKVTVAASKALDVRLEAHIAELWAI 76
            RWSPP    WK+N+DAA  K++ +  LG + R+FEG+V  +  K +    +   A L+AI
Sbjct: 1029 RWSPPAVGSWKINTDAAFDKDLGVAGLGAVLRDFEGRVLCSIIKKMHFIQDPLFAVLYAI 1088

Query: 77   LEGLKLVISLGCTMLVVESDCQLAINFINKRQRRDAISLASRRCDSVATLCTQRRDATRR 136
              GL+L  S G    V+ESDC LAI          AI+  S R      L +  RD  R 
Sbjct: 1089 QLGLQLARSEGFLTCVIESDCLLAIT---------AINTDSSRFWEGDCLVSSIRDLARE 1142

Query: 137  FSI 140
            FS+
Sbjct: 1149 FSL 1142

BLAST of Spg026448 vs. NCBI nr
Match: XP_042965942.1 (uncharacterized protein LOC122299620 [Carya illinoinensis])

HSP 1 Score: 68.9 bits (167), Expect = 7.1e-08
Identity = 41/103 (39.81%), Postives = 56/103 (54.37%), Query Frame = 0

Query: 15   NRRWSPPNGVFWKLNSDAACSKEVSMTSLGMICRNFEGKVTVAASKALDVRLEAHIAELW 74
            N  WSPP   F KLN D A   +     +G + R+  G+V +AASK      E    EL 
Sbjct: 1205 NGLWSPPPEGFLKLNVDGALFFDQKKAGIGAVLRDGRGEVILAASKMECYIEEPETIELV 1264

Query: 75   AILEGLKLVISLGCTMLVVESDCQLAINFINKRQRRDAISLAS 118
            AIL GL+L I  G + LVVESDC+L +  + +  ++DA  L +
Sbjct: 1265 AILRGLQLCIQSGISKLVVESDCKLLVEILQQEPQQDASQLGN 1307

BLAST of Spg026448 vs. NCBI nr
Match: XP_042950144.1 (uncharacterized protein LOC122282259 [Carya illinoinensis])

HSP 1 Score: 68.9 bits (167), Expect = 7.1e-08
Identity = 41/103 (39.81%), Postives = 56/103 (54.37%), Query Frame = 0

Query: 15  NRRWSPPNGVFWKLNSDAACSKEVSMTSLGMICRNFEGKVTVAASKALDVRLEAHIAELW 74
           N  WSPP   F KLN D A   +     +G + R+  G+V +AASK      E    EL 
Sbjct: 672 NGLWSPPPEGFLKLNIDGALFFDQKKAGIGAVLRDGRGEVILAASKMECYIEEPETIELV 731

Query: 75  AILEGLKLVISLGCTMLVVESDCQLAINFINKRQRRDAISLAS 118
           AIL GL+L I  G + LVVESDC+L +  + +  ++DA  L +
Sbjct: 732 AILRGLQLCIQSGISKLVVESDCKLLVEILQQEPQQDASQLGN 774

BLAST of Spg026448 vs. NCBI nr
Match: KAF5454638.1 (hypothetical protein F2P56_024287 [Juglans regia])

HSP 1 Score: 68.9 bits (167), Expect = 7.1e-08
Identity = 42/110 (38.18%), Postives = 63/110 (57.27%), Query Frame = 0

Query: 9   SSSLDRNRR---WSPPNGVFWKLNSDAACSKEVSMTSLGMICRNFEGKVTVAASKALDVR 68
           SSS    +R   W+PP   F KLN D A   +V    LG++ R+ +GK+ +AASK  +  
Sbjct: 29  SSSTQNAKRACSWNPPPRGFLKLNVDEAVFSDVRKAGLGVVLRDDKGKLVMAASKIENEV 88

Query: 69  LEAHIAELWAILEGLKLVISLGCTMLVVESDCQLAINFINKRQRRDAISL 116
                 EL A+L GL+L++ LG + L+VESDC   +  +N  Q  D++S+
Sbjct: 89  ENPSTIELLALLRGLQLIVHLGFSKLLVESDCMFLVQELNNEQ--DSLSV 136

BLAST of Spg026448 vs. ExPASy TrEMBL
Match: A0A1R3HQR7 (Ribonuclease H OS=Corchorus capsularis OX=210143 GN=CCACVL1_17615 PE=4 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 9.0e-09
Identity = 47/123 (38.21%), Postives = 64/123 (52.03%), Query Frame = 0

Query: 17   RWSPPNGVFWKLNSDAACSKEVSMTSLGMICRNFEGKVTVAASKALDVRLEAHIAELWAI 76
            RWSPP    WK+N+DAA  K++ +  LG + R+FEG+V  +  K +    +   A L+AI
Sbjct: 1029 RWSPPAVGSWKINTDAAFDKDLGVAGLGAVLRDFEGRVLCSIIKKMHFIQDPLFAVLYAI 1088

Query: 77   LEGLKLVISLGCTMLVVESDCQLAINFINKRQRRDAISLASRRCDSVATLCTQRRDATRR 136
              GL+L  S G    V+ESDC LAI          AI+  S R      L +  RD  R 
Sbjct: 1089 QLGLQLARSEGFLTCVIESDCLLAIT---------AINTDSSRFWEGDCLVSSIRDLARE 1142

Query: 137  FSI 140
            FS+
Sbjct: 1149 FSL 1142

BLAST of Spg026448 vs. ExPASy TrEMBL
Match: A0A1R3GKQ5 (Reverse transcriptase OS=Corchorus capsularis OX=210143 GN=CCACVL1_25420 PE=4 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 5.9e-08
Identity = 35/95 (36.84%), Postives = 57/95 (60.00%), Query Frame = 0

Query: 14   RNRRWSPPNGVFWKLNSDAACSKEVSMTSLGMICRNFEGKVTVAASKALDVRLEAHIAEL 73
            R   W  P+   WK+N+DA+ S       LG++ R++EGK+ V+ ++ L    ++  AE+
Sbjct: 1461 RELEWIAPSPGTWKINTDASFSSSTGDAGLGVVIRDYEGKILVSGARILFFVADSLHAEV 1520

Query: 74   WAILEGLKLVISLGCTMLVVESDCQLAINFINKRQ 109
             AIL G +L +  G T  ++ESD  LAI+ INK++
Sbjct: 1521 HAILFGFELALEHGITRCIIESDSLLAIHEINKKE 1555

BLAST of Spg026448 vs. ExPASy TrEMBL
Match: A0A1S8ABJ9 (Ribonuclease H-like superfamily protein (Fragment) OS=Citrus limon OX=2708 PE=4 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 7.7e-08
Identity = 38/89 (42.70%), Postives = 51/89 (57.30%), Query Frame = 0

Query: 18  WSPPNGVFWKLNSDAACSKEVSMTSLGMICRNFEGKVTVAASKALDVRLEAHIAELWAIL 77
           W PP+   +KLN+DAA  KE  +T LG I RN +G V  A +       +  IAE  AIL
Sbjct: 148 WHPPDPPRYKLNTDAALDKEGGVTGLGCIIRNHQGAVMAAVTSKKPYFGDVEIAEALAIL 207

Query: 78  EGLKLVISLGCTMLVVESDCQLAINFINK 107
           EGLKL   +  +  ++ESD +  +NFI K
Sbjct: 208 EGLKLAAEVALSPHLIESDSKNVVNFILK 236

BLAST of Spg026448 vs. ExPASy TrEMBL
Match: A0A6J1BQ49 (uncharacterized protein LOC111004786 OS=Momordica charantia OX=3673 GN=LOC111004786 PE=4 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 7.7e-08
Identity = 45/100 (45.00%), Postives = 55/100 (55.00%), Query Frame = 0

Query: 5   SNEDSSSLDRNRRWSPPNGVFWKLNSDAACSKEVSMTSLGMICRNFEGKVTVAASKALDV 64
           S + SS    + RW PP     K+NSDAAC    + T LG+I R+  G + VA S  L +
Sbjct: 102 SPQQSSVPPPSCRWLPPRDSAMKVNSDAACRS--TSTGLGLIIRDHFGVLLVAKSMFLPM 161

Query: 65  RLEAHIAELWAILEGLKLVISLGCTMLVVESDCQLAINFI 105
            L    AE+  ILE LKL  S   T LVVESDCQ AI  +
Sbjct: 162 PLNPLFAEIRGILEALKLAASRSYTRLVVESDCQEAIRLV 199

BLAST of Spg026448 vs. ExPASy TrEMBL
Match: A0A6J1DJL6 (uncharacterized protein LOC111021711 OS=Momordica charantia OX=3673 GN=LOC111021711 PE=4 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 3.8e-07
Identity = 39/101 (38.61%), Postives = 58/101 (57.43%), Query Frame = 0

Query: 6   NEDSSSLDRNRRWSPPNGVFWKLNSDAACSKEVSMTSLGMICRNFEGKVTVAASKALDVR 65
           N + +  D N  W+PP+  + K+N D AC    S T +G++C+N +G++ VA  + LDV 
Sbjct: 117 NSNKAGKDVN-YWAPPSPDWVKINVDTACKLSNSRTGIGVVCQNDKGEIIVAMIRKLDVF 176

Query: 66  LEAHIAELWAILEGLKLVISLGCTMLVVESDCQLAINFINK 107
            E    E  AILEGL L   L    + V+SD Q +IN I++
Sbjct: 177 YEPLQVETLAILEGLWLTQCLDLIFVQVDSDSQNSINLISQ 216

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_040996092.13.4e-1045.56LOW QUALITY PROTEIN: uncharacterized protein LOC121242268 [Juglans microcarpa x ... [more]
OMO72757.11.9e-0838.21hypothetical protein CCACVL1_17615 [Corchorus capsularis][more]
XP_042965942.17.1e-0839.81uncharacterized protein LOC122299620 [Carya illinoinensis][more]
XP_042950144.17.1e-0839.81uncharacterized protein LOC122282259 [Carya illinoinensis][more]
KAF5454638.17.1e-0838.18hypothetical protein F2P56_024287 [Juglans regia][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A1R3HQR79.0e-0938.21Ribonuclease H OS=Corchorus capsularis OX=210143 GN=CCACVL1_17615 PE=4 SV=1[more]
A0A1R3GKQ55.9e-0836.84Reverse transcriptase OS=Corchorus capsularis OX=210143 GN=CCACVL1_25420 PE=4 SV... [more]
A0A1S8ABJ97.7e-0842.70Ribonuclease H-like superfamily protein (Fragment) OS=Citrus limon OX=2708 PE=4 ... [more]
A0A6J1BQ497.7e-0845.00uncharacterized protein LOC111004786 OS=Momordica charantia OX=3673 GN=LOC111004... [more]
A0A6J1DJL63.8e-0738.61uncharacterized protein LOC111021711 OS=Momordica charantia OX=3673 GN=LOC111021... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 29..111
e-value: 1.3E-15
score: 57.3
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 25..175
e-value: 2.2E-11
score: 45.9
NoneNo IPR availablePANTHERPTHR47074BNAC02G40300D PROTEINcoord: 13..127
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 28..110
e-value: 3.66213E-13
score: 62.7168
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 26..115

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg026448.1Spg026448.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity