Spg026212 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg026212
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	cytochrome P450 CYP749A22-like
Location	scaffold7: 3663353 .. 3665924 (+)
RNA-Seq Expression	Spg026212
Synteny	Spg026212

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCGCTTTCGGTTTCAGTGACACTACCGTCCTCCTCCTCGGCCTTCTCTGTGCTTCCATTTTCTACCTATTCTTCAAGCTTTTTCTCAAGCTATGCTGGAAACCTTTTCGGATTCGGCGTTTTATGCGATCGCAGGGGATTCAGGGTCCTCCTTCCAGCTTCATCTATGGAAACCTCTTTCAAATCATGAAGATGAAGATGGAATCCATGCGCACCTCTCTCGATATCACCGACCATAACATACTTCCCAGAGTTCATCCTCATGTTCACTTGTGGATCAACAAATATGGTTCGTCTTCACTCTCCGAGTCCGAGCTTCAGCTTAATTTCATTGTTTTCGGTTTGATTGACTGATTTGAGTTACAGGGAAAAACTTCCTTCAGTGGAATGGGAATATGGCTCAGTTGATCGTTTCGGATCCGGAGACGATCAAGGAGATACTTCATAATCAAGAACGAGCTATTTTCAAAATGACTCTCAACGAAGATTCTCGGAGGATATTCGGGAATGGACAGTTGACTTCCGAAGGAGAAAAATGGGCCAAGATTCGAAGAATTGCTGATTATGCTTTCCATGGGGAACTCCTAAAAGTTAGTGGGATTTGGTTCTGTTTAATGATTTTACTGATTCAGTTGTGCCTTTAGATTCAATTTCTTGAAAATGGTTTTGCAGAAGATGGGGCCAACCATGGTTTCTTGTGCTGAGGCGATGGTGGAAAAGTGGAAGAAGCAACCTGAAGGCAAAGAGGTTGATTTGTTTCAAGAGTTTAGGGTGTTAACTTCAGATATCATTGCACATACAGCCTTTGGAAGCAGTTTTTTGGAAGGGAAATTCATTTTTGAGACTCTGAATAAACTGGCCATGATATTTTTTAAGAATCAATTCAAACGAGGCATTCCCGGAATCAGGTGTTCTTTTAAGTATTTTTGAAACAATCTGCTTACTTGCATTGATGCTATCTAAGTAAAACTAAATTATTAAGTTTGTGTGCAGCAAGTTATTCAAATCAAAGGATGCTAGGGAGGGAGATGAGCTAGCAAGAAAGATGCAAAGCTCCATAATTTCAATAATAAAAAATAGAGAAGAGAAGGTGATAAGTGGTGAAGCAGAGAACTATGGTAATGATTTTCTTGCATTACTTTTGAAGGCAAAGAATAATTCTGACCAGAGGCAGAGGATTTCTGTTGAGGATGTGGTAGATGAATGCAAAGCAATGTACTTTTCGGGGCAAGAAACTACGGGTGTTTTGCTTTCTTGGACTGCGTTTCTGTTGGCAACTCATGTGCACTGGCAAGAGGAAGCAAGAAAGGAAGTGTTGGATATGTTTGGCAACGATAATCCAACTTTAGAGGGCATCACAAAGTTAAAGATTGTAAGCATGTCTCTCCATTATTAAGGCCATTACTTTTTCATAGAGTAGGCTTAAGATTTGTTGGTGTAGCAGAACTCCGAAAAGGGCCATCATGGATGATAAACATCCAATTCCCATATGCTTGATTTAGTAGTCTTTCATTAAGGGTTTAGATTGAGATTTTGTAATTTATCTTTATCTAGAATAGAGATTTTAGTTCAGGTTTTTCCAGTCTTATTTTAGATTTACACTGTTCAAATCTGCAATTGGATTCAGGAAGGTTTGGTTTAAACTGCCATTCTTGGAAAATGAAAATCTTCCCAACTTTCTATAGGCTAACATGAGAATCTGCCAACCATAGCTTAGTTAGTTAAAACATATACTTTGAGGTTCGAAAACTTCATCCCCATTTCCTCTCTTTTACAATGACTGCTTTTCTTCCATTATGAATCAAGAACTGAGAGTTGTTTTCACTCTTGTCTTATAGATGAGCATGATCATCAAGGAAACGTTAAGATTATATCCTCCAACCCAAGTCATGTCAAGGTTGGTTAAGAAGGGAGTCAGACTGGGGAAGCTCGTTCTCCCAGCCAACATTCAAGTTAGCATCTCAACGATTGCAGTTCATCATGATACTGCAATATGGGGTGAAGATGCTCATGAGTTCAAACCAGAAAGATTTTCAGAAGGAAAAGCTGAAGATATCCCATCAGGTGCATACATACCATTTGGGTTGGGTCCTCGAAACTGCGTTGGCAATATGTTGGCCATCAACGAAACTAAGATTACATTATCGATGATTCTACAACGATTTTCTTTCACCATTTCCCCAGGCCACGTCCACGCACCGTACCAGTTCCTCACCAATTGCCCGCAACATGGAGTTCGGGTGAAGCTTCAGTCTCTGACTGGATGAAAGGGGAAGGAAGATGTATAATTGTATATGGTTTAACTGCCCACTCAGACACCTTCCATTGTCGGGCCCTTTTTTAGGTTTTGGTAAATTAAACCTTTGAATCAATTCATCCTCTCTTTATGTTGTTTCACTATATTTAGTCTTAGTTTTGTATTTTGTCCATGTACTCTATTGAGTTGTGTTTCATGTTCCTTCGTCCTTTTCTTCTATTAGCTCAACGACTAAAATATCAATAATAGTTTTCTTGAGTTTGAGATTGCAATTTTCCCTTCTACTATTGTGAAATTATAATAATTTTACTG

mRNA sequence

ATGGCCGCTTTCGGTTTCAGTGACACTACCGTCCTCCTCCTCGGCCTTCTCTGTGCTTCCATTTTCTACCTATTCTTCAAGCTTTTTCTCAAGCTATGCTGGAAACCTTTTCGGATTCGGCGTTTTATGCGATCGCAGGGGATTCAGGGTCCTCCTTCCAGCTTCATCTATGGAAACCTCTTTCAAATCATGAAGATGAAGATGGAATCCATGCGCACCTCTCTCGATATCACCGACCATAACATACTTCCCAGAGTTCATCCTCATTGGAATGGGAATATGGCTCAGTTGATCGTTTCGGATCCGGAGACGATCAAGGAGATACTTCATAATCAAGAACGAGCTATTTTCAAAATGACTCTCAACGAAGATTCTCGGAGGATATTCGGGAATGGACAGTTGACTTCCGAAGGAGAAAAATGGGCCAAGATTCGAAGAATTGCTGATTATGCTTTCCATGGGGAACTCCTAAAAAAGATGGGGCCAACCATGGTTTCTTGTGCTGAGGCGATGGTGGAAAAGTGGAAGAAGCAACCTGAAGGCAAAGAGGTTGATTTGTTTCAAGAGTTTAGGGTCAAGTTATTCAAATCAAAGGATGCTAGGGAGGGAGATGAGCTAGCAAGAAAGATGCAAAGCTCCATAATTTCAATAATAAAAAATAGAGAAGAGAAGGTGATAAGTGGTGAAGCAGAGAACTATGGTAATGATTTTCTTGCATTACTTTTGAAGGCAAAGAATAATTCTGACCAGAGGCAGAGGATTTCTGTTGAGGATGTGGTAGATGAATGCAAAGCAATGTACTTTTCGGGGCAAGAAACTACGGGTGTTTTGCTTTCTTGGACTGCGTTTCTGTTGGCAACTCATGTGCACTGGCAAGAGGAAGCAAGAAAGGAAGTGTTGGATATGTTTGGCAACGATAATCCAACTTTAGAGGGCATCACAAAGTTAAAGATTATGAGCATGATCATCAAGGAAACGTTAAGATTATATCCTCCAACCCAAGTCATGTCAAGGTTGGTTAAGAAGGGAGTCAGACTGGGGAAGCTCGTTCTCCCAGCCAACATTCAAGTTAGCATCTCAACGATTGCAGTTCATCATGATACTGCAATATGGGGTGAAGATGCTCATGAGTTCAAACCAGAAAGATTTTCAGAAGGAAAAGCTGAAGATATCCCATCAGGTGCATACATACCATTTGGGTTGGGTCCTCGAAACTGCGTTGGCAATATGTTGGCCATCAACGAAACTAAGATTACATTATCGATGATTCTACAACGATTTTCTTTCACCATTTCCCCAGGCCACGTCCACGCACCGTACCAGTTCCTCACCAATTGCCCGCAACATGGAGTTCGGGTGAAGCTTCAGTCTCTGACTGGATGA

Coding sequence (CDS)

ATGGCCGCTTTCGGTTTCAGTGACACTACCGTCCTCCTCCTCGGCCTTCTCTGTGCTTCCATTTTCTACCTATTCTTCAAGCTTTTTCTCAAGCTATGCTGGAAACCTTTTCGGATTCGGCGTTTTATGCGATCGCAGGGGATTCAGGGTCCTCCTTCCAGCTTCATCTATGGAAACCTCTTTCAAATCATGAAGATGAAGATGGAATCCATGCGCACCTCTCTCGATATCACCGACCATAACATACTTCCCAGAGTTCATCCTCATTGGAATGGGAATATGGCTCAGTTGATCGTTTCGGATCCGGAGACGATCAAGGAGATACTTCATAATCAAGAACGAGCTATTTTCAAAATGACTCTCAACGAAGATTCTCGGAGGATATTCGGGAATGGACAGTTGACTTCCGAAGGAGAAAAATGGGCCAAGATTCGAAGAATTGCTGATTATGCTTTCCATGGGGAACTCCTAAAAAAGATGGGGCCAACCATGGTTTCTTGTGCTGAGGCGATGGTGGAAAAGTGGAAGAAGCAACCTGAAGGCAAAGAGGTTGATTTGTTTCAAGAGTTTAGGGTCAAGTTATTCAAATCAAAGGATGCTAGGGAGGGAGATGAGCTAGCAAGAAAGATGCAAAGCTCCATAATTTCAATAATAAAAAATAGAGAAGAGAAGGTGATAAGTGGTGAAGCAGAGAACTATGGTAATGATTTTCTTGCATTACTTTTGAAGGCAAAGAATAATTCTGACCAGAGGCAGAGGATTTCTGTTGAGGATGTGGTAGATGAATGCAAAGCAATGTACTTTTCGGGGCAAGAAACTACGGGTGTTTTGCTTTCTTGGACTGCGTTTCTGTTGGCAACTCATGTGCACTGGCAAGAGGAAGCAAGAAAGGAAGTGTTGGATATGTTTGGCAACGATAATCCAACTTTAGAGGGCATCACAAAGTTAAAGATTATGAGCATGATCATCAAGGAAACGTTAAGATTATATCCTCCAACCCAAGTCATGTCAAGGTTGGTTAAGAAGGGAGTCAGACTGGGGAAGCTCGTTCTCCCAGCCAACATTCAAGTTAGCATCTCAACGATTGCAGTTCATCATGATACTGCAATATGGGGTGAAGATGCTCATGAGTTCAAACCAGAAAGATTTTCAGAAGGAAAAGCTGAAGATATCCCATCAGGTGCATACATACCATTTGGGTTGGGTCCTCGAAACTGCGTTGGCAATATGTTGGCCATCAACGAAACTAAGATTACATTATCGATGATTCTACAACGATTTTCTTTCACCATTTCCCCAGGCCACGTCCACGCACCGTACCAGTTCCTCACCAATTGCCCGCAACATGGAGTTCGGGTGAAGCTTCAGTCTCTGACTGGATGA

Protein sequence

MAAFGFSDTTVLLLGLLCASIFYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKMESMRTSLDITDHNILPRVHPHWNGNMAQLIVSDPETIKEILHNQERAIFKMTLNEDSRRIFGNGQLTSEGEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGKEVDLFQEFRVKLFKSKDAREGDELARKMQSSIISIIKNREEKVISGEAENYGNDFLALLLKAKNNSDQRQRISVEDVVDECKAMYFSGQETTGVLLSWTAFLLATHVHWQEEARKEVLDMFGNDNPTLEGITKLKIMSMIIKETLRLYPPTQVMSRLVKKGVRLGKLVLPANIQVSISTIAVHHDTAIWGEDAHEFKPERFSEGKAEDIPSGAYIPFGLGPRNCVGNMLAINETKITLSMILQRFSFTISPGHVHAPYQFLTNCPQHGVRVKLQSLTG

Homology

[Close]

BLAST of Spg026212 vs. NCBI nr
Match: XP_022149008.1 (cytochrome P450 CYP749A22-like [Momordica charantia])

HSP 1 Score: 689.9 bits (1779), Expect = 1.5e-194
Identity = 357/511 (69.86%), Postives = 399/511 (78.08%), Query Frame = 0


Query: 9   TTVLLLGLLCA-SIFYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMK 68
           T V LLG+L A SIFY    + LKL WKP RI+R MRSQG+ GPPS+FIYGN+ Q+MKMK
Sbjct: 5   TAVFLLGVLSASSIFY----VLLKLWWKPLRIQRIMRSQGVYGPPSNFIYGNMIQVMKMK 64

Query: 69  MESMRTSLDITDHNILPRVHPH--------------WNGNMAQLIVSDPETIKEILHNQE 128
            ESMRTSLDI+DH+ILPRV+PH              WNG  AQLIVSDPETIKEILHN+E
Sbjct: 65  FESMRTSLDISDHHILPRVNPHIHLWIDKYGKNFIQWNGTQAQLIVSDPETIKEILHNRE 124

Query: 129 RAIFKMTLNEDSRRIFGNGQLTSEGEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVE 188
           RAI K+ L  D+RRIFG+G  TS+GEKWAK RRIADYAFHGELLK M PTMVSCAEAMVE
Sbjct: 125 RAITKIDLGGDARRIFGDGLSTSDGEKWAKARRIADYAFHGELLKNMMPTMVSCAEAMVE 184

Query: 189 KWKKQPEGKEVDLFQEFRV----------------------------------------- 248
           KWKK  EGKE+DLF+EF++                                         
Sbjct: 185 KWKKNDEGKELDLFEEFKLLTSDIIAHTAFGSSYLEGKFIFETLSKLSMILFKNQFKRRI 244

Query: 249 ----KLFKSKDAREGDELARKMQSSIISIIKNREEKVISGEAENYGNDFLALLLKAKNNS 308
               KLFK+KDAREGDEL  +M++SI+SIIK REEKV+SGEA+NYGNDFL LLLKAKN+ 
Sbjct: 245 PGFSKLFKTKDAREGDELEMRMKNSIVSIIKKREEKVMSGEADNYGNDFLGLLLKAKNDP 304

Query: 309 DQRQRISVEDVVDECKAMYFSGQETTGVLLSWTAFLLATHVHWQEEARKEVLDMFGNDNP 368
           D RQRISV+DVVDECK +YF+GQETT VLLSWT+FLLATHV WQEEARKEV DMFGN NP
Sbjct: 305 DPRQRISVDDVVDECKTVYFAGQETTNVLLSWTSFLLATHVDWQEEARKEVFDMFGNTNP 364

Query: 369 TLEGITKLKIMSMIIKETLRLYPPTQVMSRLVKKGVRLGKLVLPANIQVSISTIAVHHDT 428
           TLEGI KLKIMSMIIKE+LRLYPP   MSR+VKK VRLGKLVLPA+IQVSIS IAVHHDT
Sbjct: 365 TLEGIAKLKIMSMIIKESLRLYPPATPMSRMVKKEVRLGKLVLPASIQVSISNIAVHHDT 424

Query: 429 AIWGEDAHEFKPERFSEGKAEDIPSGAYIPFGLGPRNCVGNMLAINETKITLSMILQRFS 460
           A WGEDAHEFKPERFSEG A+DIPS AYIPFGLGPRNCVGN+LA+NETKI LSMILQRFS
Sbjct: 425 ATWGEDAHEFKPERFSEGTAKDIPSAAYIPFGLGPRNCVGNILAVNETKIALSMILQRFS 484

[Close]

BLAST of Spg026212 vs. NCBI nr
Match: XP_038892924.1 (cytochrome P450 CYP749A22-like isoform X1 [Benincasa hispida])

HSP 1 Score: 491.5 bits (1264), Expect = 7.9e-135
Identity = 261/504 (51.79%), Postives = 327/504 (64.88%), Query Frame = 0


Query: 17  LCASIFYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKMESMRTSLD 76
           L   + +   KLF+KL W P RI+RFMRSQGIQGP   FI GN   +   +M++M T +D
Sbjct: 13  LFGCLLFGVIKLFVKLWWTPMRIQRFMRSQGIQGPSYKFIQGNTRDVYTKRMQAMATPMD 72

Query: 77  ITDHNILPRVHP--------------HWNGNMAQLIVSDPETIKEILHNQERAIFKMTLN 136
           ++ H+ILPRV P               W G  AQLI++DPE IKE+LH++++   K  L 
Sbjct: 73  LS-HHILPRVMPSVHSWLNLYGRNFLQWFGVDAQLIITDPEMIKEVLHDRQKNFPKAKLQ 132

Query: 137 EDSRRIFGNGQLTSEGEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGK 196
               RIFGNG  T+EGE+WAK R+IA++AFHG+ LK M PTM+ C E M E WK   EGK
Sbjct: 133 GHIHRIFGNGLATAEGERWAKSRKIANFAFHGDSLKNMIPTMIECGEKMTEGWKNY-EGK 192

Query: 197 EVDLFQEFRV---------------------------------------------KLFKS 256
           E+D+F+EF+V                                             K+ KS
Sbjct: 193 ELDVFKEFKVYTLDVISHTAFGSSYQQGKDIFHMLQELCELSIRNGYKIKLPIISKILKS 252

Query: 257 KDAREGDELARKMQSSIISIIKNREEKVISGEAENYGNDFLALLLKAKNNSDQRQRISVE 316
           KD  EG+ L ++M+   + IIK REEK+ +GEA+ YGNDFL LL+KAKN+ +  QRISVE
Sbjct: 253 KDDYEGERLEKRMKDCFMEIIKGREEKLRNGEADGYGNDFLGLLVKAKNDPEISQRISVE 312

Query: 317 DVVDECKAMYFSGQETTGVLLSWTAFLLATHVHWQEEARKEVLDMFGNDNPTLEGITKLK 376
           D+VDECK  YF+G ETT VLL+WT FLLA H  WQEEAR EVL +FG+ NPTLEG+ KL+
Sbjct: 313 DIVDECKTFYFAGHETTNVLLAWTMFLLALHKEWQEEARNEVLKVFGDKNPTLEGLPKLR 372

Query: 377 IMSMIIKETLRLYPPTQVMSRLVKKGVRLGKLVLPANIQVSISTIAVHHDTAIWGEDAHE 436
           +M +II E LRLYPP   +SRLV+K VRLG LV+P   ++ I T+AVHHD   WGEDAHE
Sbjct: 373 MMMIIINECLRLYPPAMTVSRLVEKEVRLGNLVVPTMTKLVIPTVAVHHDMTFWGEDAHE 432

Query: 437 FKPERFSE--GKAEDIPSGAYIPFGLGPRNCVGNMLAINETKITLSMILQRFSFTISPGH 460
           FKPERF E  GKA +  S AYIPFGLGPRNCVG   A+NE KI +SMILQR+SF +SP +
Sbjct: 433 FKPERFLEGVGKATESNSAAYIPFGLGPRNCVGMNFAMNEAKIAMSMILQRYSFDLSPAY 492

[Close]

BLAST of Spg026212 vs. NCBI nr
Match: XP_038892699.1 (cytochrome P450 CYP749A22-like [Benincasa hispida] >XP_038892700.1 cytochrome P450 CYP749A22-like [Benincasa hispida])

HSP 1 Score: 488.8 bits (1257), Expect = 5.1e-134
Identity = 257/504 (50.99%), Postives = 325/504 (64.48%), Query Frame = 0


Query: 17  LCASIFYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKMESMRTSLD 76
           L   + +   KLF KL W P  I+RFMRSQGI+GP   FI GN   +   +M +M T +D
Sbjct: 13  LFGCLLFCVIKLFFKLWWIPMSIQRFMRSQGIRGPSYKFIQGNTSDVYIKRMHAMATPMD 72

Query: 77  ITDHNILPRVHP--------------HWNGNMAQLIVSDPETIKEILHNQERAIFKMTLN 136
           ++ HNILPRV P               W G  AQLI++DPE IKE+LH++++   K  L 
Sbjct: 73  LS-HNILPRVMPSIYSWLSLYGRNNLQWFGFDAQLIITDPEMIKEVLHDRQKNFPKAKLQ 132

Query: 137 EDSRRIFGNGQLTSEGEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGK 196
               RIFGNG +T+EGE+WAK R+IA++AFHG+ LK M PTM+ C + M+E WK   EGK
Sbjct: 133 GHIHRIFGNGLVTAEGERWAKSRKIANFAFHGDSLKNMIPTMIECGDKMIEGWKNY-EGK 192

Query: 197 EVDLFQEFRV---------------------------------------------KLFKS 256
           E+D+F+EF+V                                             K+ KS
Sbjct: 193 ELDVFKEFKVYTLDVISHTAFGSSYQQGKDIFHILQELCELSIRNGYKIKLPVISKILKS 252

Query: 257 KDAREGDELARKMQSSIISIIKNREEKVISGEAENYGNDFLALLLKAKNNSDQRQRISVE 316
           KD  EG  L ++M+   + IIK REEK+ +GEAE YGNDFL LL+KAKN+ +  Q ISVE
Sbjct: 253 KDDYEGKRLEKRMKDCFMEIIKAREEKLRNGEAEGYGNDFLGLLIKAKNDPETSQSISVE 312

Query: 317 DVVDECKAMYFSGQETTGVLLSWTAFLLATHVHWQEEARKEVLDMFGNDNPTLEGITKLK 376
           D+VDECK  YF+G ETT VLL+WT FLLA H  WQEEAR EV  +FG+ NPTLEG+ KL+
Sbjct: 313 DIVDECKTFYFAGHETTNVLLTWTMFLLALHKEWQEEARNEVFKVFGDKNPTLEGLPKLR 372

Query: 377 IMSMIIKETLRLYPPTQVMSRLVKKGVRLGKLVLPANIQVSISTIAVHHDTAIWGEDAHE 436
            M +II E LRLYPP   ++R V+K VRLG +V+P  I ++I T+AVHHDTA WGEDAHE
Sbjct: 373 TMMIIINECLRLYPPAMSVARRVEKEVRLGSMVVPTTIMLTIPTVAVHHDTAFWGEDAHE 432

Query: 437 FKPERFSE--GKAEDIPSGAYIPFGLGPRNCVGNMLAINETKITLSMILQRFSFTISPGH 460
           FKPERFSE  GKA +    AYIPFGLGPR+CVG   A+NE KI +SMILQR+SF++SP +
Sbjct: 433 FKPERFSEGVGKATESNLAAYIPFGLGPRSCVGMNFAMNEAKIAMSMILQRYSFSLSPAY 492

[Close]

BLAST of Spg026212 vs. NCBI nr
Match: XP_004135430.2 (cytochrome P450 CYP749A22 isoform X1 [Cucumis sativus] >KAE8648770.1 hypothetical protein Csa_008223 [Cucumis sativus])

HSP 1 Score: 487.3 bits (1253), Expect = 1.5e-133
Identity = 256/503 (50.89%), Postives = 327/503 (65.01%), Query Frame = 0


Query: 17  LCASIFYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKMESMRTSLD 76
           L   + +  FKLF+KL W P RI+RFMRSQGIQGP   FI GN   +   +M++M T +D
Sbjct: 13  LSGCLLFGVFKLFVKLWWTPMRIQRFMRSQGIQGPSFKFIQGNTRDVYIKRMQAMATPMD 72

Query: 77  ITDHNILPRVHP--------------HWNGNMAQLIVSDPETIKEILHNQERAIFKMTLN 136
           ++ HNIL RV P               W+G  AQL+++DPE IKE+LH+++++  K  L 
Sbjct: 73  LS-HNILHRVMPCVHSWLNLYGRNYLQWSGVDAQLMITDPEMIKEVLHDRQKSFPKAKLK 132

Query: 137 EDSRRIFGNGQLTSEGEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGK 196
               RIFGNG  T+EG++WA  RRIA++AFHG+ LK M PTM+ C E M+E WK   EGK
Sbjct: 133 GHIHRIFGNGLATAEGQRWANSRRIANFAFHGDSLKNMIPTMIECGEKMIEGWKNY-EGK 192

Query: 197 EVDLFQEFRV---------------------------------------------KLFKS 256
           E+D F+EF+V                                             K+ KS
Sbjct: 193 ELDAFKEFKVFTLDVISHTAFGSSYQQGKKIFHMLRELCELSIRNGYKIRLPIISKILKS 252

Query: 257 KDAREGDELARKMQSSIISIIKNREEKVISGEAENYGNDFLALLLKAKNNSDQRQRISVE 316
           KD  EG+ L ++++   + IIK REEK+ +GEA +YGNDFL LL+KAKN+ +  Q IS+E
Sbjct: 253 KDDYEGERLGKRLKDCFMEIIKEREEKLRNGEANDYGNDFLGLLIKAKNDPETSQCISME 312

Query: 317 DVVDECKAMYFSGQETTGVLLSWTAFLLATHVHWQEEARKEVLDMFGNDNPTLEGITKLK 376
           D+VDECK  YF+G ETT VLL+WT  LLA H  WQE+AR EVLD+FG++NPTLEG+ KLK
Sbjct: 313 DIVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEVLDVFGHNNPTLEGLPKLK 372

Query: 377 IMSMIIKETLRLYPPTQVMSRLVKKGVRLGKLVLPANIQVSISTIAVHHDTAIWGEDAHE 436
            M+MII E LRLYPP   ++R V+K VRLG LV+P    ++I T+AVHHDT  WGEDAHE
Sbjct: 373 TMAMIINECLRLYPPAMPVARRVEKEVRLGNLVVPTATMLTIPTVAVHHDTTFWGEDAHE 432

Query: 437 FKPERFSE--GKAEDIPSGAYIPFGLGPRNCVGNMLAINETKITLSMILQRFSFTISPGH 459
           FKPERFSE  GKA +  S AYIPFGLGPRNCVG   A+NE KI +SMILQR+SF +SP +
Sbjct: 433 FKPERFSEGVGKATENNSAAYIPFGLGPRNCVGMNFAMNEAKIAMSMILQRYSFRLSPAY 492

[Close]

BLAST of Spg026212 vs. NCBI nr
Match: XP_008446466.1 (PREDICTED: cytochrome P450 CYP749A22-like [Cucumis melo])

HSP 1 Score: 486.5 bits (1251), Expect = 2.5e-133
Identity = 252/503 (50.10%), Postives = 326/503 (64.81%), Query Frame = 0


Query: 17  LCASIFYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKMESMRTSLD 76
           L   + ++ FKLF+KL W P RI+RFMRSQGIQGP   FI GN   +   +M++M T +D
Sbjct: 13  LSGCLLFVVFKLFVKLWWTPMRIQRFMRSQGIQGPSYKFIQGNTRDVYMKRMQAMATPMD 72

Query: 77  ITDHNILPRVHP--------------HWNGNMAQLIVSDPETIKEILHNQERAIFKMTLN 136
           ++ HNILPRV P               W G  AQL+++DPE IKE+LH+++++  K  L 
Sbjct: 73  LS-HNILPRVMPSVHSWLNLYGRNYLQWAGVDAQLMITDPEMIKEVLHDRQKSFPKAKLK 132

Query: 137 EDSRRIFGNGQLTSEGEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGK 196
               RIFGNG  T+EG++W   RRIA++AFHG+ LK M PTM+ C E M+E WK   EGK
Sbjct: 133 GHIDRIFGNGLATAEGQRWVNSRRIANFAFHGDSLKNMIPTMIECGEKMIEGWKNY-EGK 192

Query: 197 EVDLFQEFRV---------------------------------------------KLFKS 256
           E+D F+EF+V                                             K+ KS
Sbjct: 193 ELDAFKEFKVFTLDVISHTAFGSSYQQGKKIFHMLQELCDLSIRNGYKIKLPIISKILKS 252

Query: 257 KDAREGDELARKMQSSIISIIKNREEKVISGEAENYGNDFLALLLKAKNNSDQRQRISVE 316
           KD  EG+ L ++++   + IIK REEK+ + EA +YGNDFL LL+KAKN+ +  Q IS+E
Sbjct: 253 KDDYEGERLEKRLKDCFMEIIKEREEKLRNDEASDYGNDFLGLLIKAKNDPETSQSISME 312

Query: 317 DVVDECKAMYFSGQETTGVLLSWTAFLLATHVHWQEEARKEVLDMFGNDNPTLEGITKLK 376
           D+VDECK  YF+G ETT VLL+WT  LLA H  WQE+AR EV D+FG+++PTLEG+ KLK
Sbjct: 313 DIVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEVFDVFGHNHPTLEGLPKLK 372

Query: 377 IMSMIIKETLRLYPPTQVMSRLVKKGVRLGKLVLPANIQVSISTIAVHHDTAIWGEDAHE 436
            M+MII E LRLYPP   ++R V+K VRLG LV+P    ++I T+AVHHDT  WG+DAHE
Sbjct: 373 AMAMIINECLRLYPPAMTIARRVEKEVRLGNLVVPTTTLLAIPTVAVHHDTKFWGDDAHE 432

Query: 437 FKPERFSE--GKAEDIPSGAYIPFGLGPRNCVGNMLAINETKITLSMILQRFSFTISPGH 459
           FKPERFSE  GKA +  S AY+PFGLGPRNCVG   A NE KI +SMILQR+SF++SP +
Sbjct: 433 FKPERFSEGIGKATEKNSAAYLPFGLGPRNCVGMNFATNEAKIAMSMILQRYSFSLSPAY 492

[Close]

BLAST of Spg026212 vs. ExPASy Swiss-Prot
Match: H2DH17 (Cytochrome P450 CYP749A22 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 414.8 bits (1065), Expect = 1.2e-114
Identity = 219/508 (43.11%), Postives = 313/508 (61.61%), Query Frame = 0


Query: 13  LLGLLCASIFYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKMESMR 72
           LL  LC  + ++F +    + W P R++R  R QGI+GP   F+YGN  +I+ M+ ESM 
Sbjct: 19  LLSSLCVFLLFVFIRFLNDIWWTPIRLQRVFRQQGIRGPSYGFLYGNTKEILNMRKESMS 78

Query: 73  TSLDITDHNILPRVHPH--------------WNGNMAQLIVSDPETIKEILHNQERAIFK 132
             +D   HNI PR+ PH              W G  AQ +V+  + +KE +  +++A  K
Sbjct: 79  RPMDYLSHNIFPRLQPHLYSWLNIYGKNFLNWYGPRAQFVVTQVDFVKETM-IKDQAYPK 138

Query: 133 MTLNEDSRRIFGNGQLTSEGEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQ 192
           M     ++++ G+G +TS+G+KWAK RR+A++AFH E LK M P M++  E M+++W KQ
Sbjct: 139 MDPEWFAKKLLGDGIVTSKGKKWAKHRRLANHAFHAESLKSMTPAMIASVEMMLKRW-KQ 198

Query: 193 PEGKEVDLFQEF---------------------------------------RVKL----- 252
            EG+E+D+FQEF                                       +VKL     
Sbjct: 199 HEGREIDVFQEFKILTSEVISRTAFGSSYLDGKDIFDRLTQLGIIITRNSYKVKLPGISL 258

Query: 253 -FKSKDAREGDELARKMQSSIISIIKNRE-EKVISGEAENYGNDFLALLLKAKNNSDQRQ 312
            +KS D  E ++L + +  SI+ I++ RE E  +SGE  ++G DFL LL+KA N++D++ 
Sbjct: 259 FYKSNDEIEAEKLDQGLYDSILRIMEKREKESTMSGEVGSFGTDFLGLLMKAMNDADEKN 318

Query: 313 RISVEDVVDECKAMYFSGQETTGVLLSWTAFLLATHVHWQEEARKEVLDMFGNDNPTLEG 372
           RI+ +DVVDECK  Y +GQETT  LL+W  FLL  H  WQE+AR+EVL++FG + P  +G
Sbjct: 319 RITAQDVVDECKTFYVAGQETTTTLLAWVIFLLGIHTDWQEKARQEVLNLFGQEIPNSDG 378

Query: 373 ITKLKIMSMIIKETLRLYPPTQVMSRLVKKGVRLGKLVLPANIQVSISTIAVHHDTAIWG 432
           + KLK ++MII ETLRLYPP   ++R VK+  + GKL LPAN+ + + T+A+HHD  IWG
Sbjct: 379 LAKLKTVNMIINETLRLYPPVIFLTRKVKEETKFGKLTLPANVHIVVPTLALHHDEQIWG 438

Query: 433 EDAHEFKPERFSEG--KAEDIPSGAYIPFGLGPRNCVGNMLAINETKITLSMILQRFSFT 459
           +DA  FKPERFS+G  KA +  + A+ PFGLGPR+CVG   A NE KI L+MILQ +SF 
Sbjct: 439 DDALLFKPERFSQGVAKATNNNAAAFFPFGLGPRSCVGLNFATNEAKIALAMILQCYSFA 498

[Close]

BLAST of Spg026212 vs. ExPASy Swiss-Prot
Match: W8JWW3 (Cytochrome P450 72A225 OS=Catharanthus roseus OX=4058 GN=CYP72A225 PE=2 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 3.9e-76
Identity = 178/517 (34.43%), Postives = 274/517 (53.00%), Query Frame = 0


Query: 14  LGLLCASIFYLFFKLFLKLCW-KPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKMESMR 73
           + +L    F L     L   W KP ++ + +R  G++G     + G+L +  +M  E+  
Sbjct: 25  ISVLGTCFFLLLAWKMLNWAWIKPKKLEKRLRQLGLKGNSYRLLIGDLGETSQMDKEAKS 84

Query: 74  TSLDITDHNILPRVHPH--------------WNGNMAQLIVSDPETIKEILHNQERAIFK 133
             ++ T+H I+PR+ P               W G    +I++DPE IKE+L   +   ++
Sbjct: 85  KPINFTNH-IIPRIMPFLHKIIQSHGKNPFIWQGPNPAVIITDPELIKEVL--TKNYTYQ 144

Query: 134 MTLNEDSRRIFGNGQLTSEGEKWAKIRRIADYAFHGELLKKMGPTM-VSCAEAMVEKWKK 193
              ++D  ++   G    + +KWAK RR+ + AFH E LK M P   +SC+E M++KW+K
Sbjct: 145 KPPSDDLGKLISQGVAAYDKDKWAKHRRLLNPAFHIEKLKHMLPAFHLSCSE-MLKKWEK 204

Query: 194 ----------------------------------QPEGKEVDLFQ----EFRVKLFKS-- 253
                                               EGK +   Q    E  +++ +S  
Sbjct: 205 LASASAEGSFELDVWPYLQDMTSDVISRTAFGSNLEEGKNIFKLQKEILEHILQILQSFY 264

Query: 254 ---------KDAREGDELARKMQSSIISIIKNREEKVISGEAENYGNDFLALLLKAK--- 313
                    K  R   ++++++ SS+++II  R E + +GE  N  +D L +LL +    
Sbjct: 265 FPGMLYLPTKRIRRMKKISKEVHSSVLNIIMKRVEAMKAGEPSN--DDLLGILLDSNFKE 324

Query: 314 ------NNSDQRQRISVEDVVDECKAMYFSGQETTGVLLSWTAFLLATHVHWQEEARKEV 373
                 NNS++   +S E+V++ECK  YF+GQETT VLL WT  LL+ H  WQ  AR EV
Sbjct: 325 IENDNGNNSNKNCGMSFEEVIEECKLFYFAGQETTAVLLVWTLLLLSKHPEWQMRARDEV 384

Query: 374 LDMFGNDNPTLEGITKLKIMSMIIKETLRLYPPTQVMSRLVKKGVRLGKLVLPANIQVSI 433
           L +FGN  P  + +  LK+++M+I E LRLYPP  +MSR++ +  +LG L LP  I + +
Sbjct: 385 LQIFGNSEPDFDALNHLKVITMVINEVLRLYPPGFMMSRVIHEDTKLGSLSLPGGISIFL 444

Query: 434 STIAVHHDTAIWGEDAHEFKPERFSEGKAEDIPSG-AYIPFGLGPRNCVGNMLAINETKI 456
            TI +HHD  +WG+DA EFKPERFSEG ++       Y PF  GPR C+GN  A+ E K+
Sbjct: 445 PTIMLHHDEELWGDDAKEFKPERFSEGISKATKGQFIYFPFSGGPRICIGNNFAMLEAKM 504

[Close]

BLAST of Spg026212 vs. ExPASy Swiss-Prot
Match: H1A988 (11-oxo-beta-amyrin 30-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP72A154 PE=1 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 3.3e-75
Identity = 182/516 (35.27%), Postives = 275/516 (53.29%), Query Frame = 0


Query: 12  LLLGLLCASIFYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSSFIYGNLFQ--IMKMKME 71
           ++L ++ A+I      +   L  +P R+ R +R+QG+ G P      N  Q  ++K++ E
Sbjct: 12  VVLTVILAAIPIWVCHMVNTLWLRPKRLERHLRAQGLHGDPYKLSLDNSKQTYMLKLQQE 71

Query: 72  SMRTSLDITDHNILPR--------VHPH------WNGNMAQLIVSDPETIKEILHNQERA 131
           +   S+ ++  +  PR        VH +      W G   ++I++DPE IKE+  N+ + 
Sbjct: 72  AQSKSIGLSKDDAAPRIFSLAHQTVHKYGKNSFAWEGTAPKVIITDPEQIKEVF-NKIQD 131

Query: 132 IFKMTLNEDSRRIFGNGQLTSEGEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVEKW 191
             K  LN  ++ I   G +  EG+KWAK R+I + AFH E LK M P        M+ KW
Sbjct: 132 FPKPKLNPIAKYI-SIGLVQYEGDKWAKHRKIINPAFHLEKLKGMLPAFSHSCHEMISKW 191

Query: 192 K--KQPEGK-EVDLFQEFR-------------------VKLFK----------------- 251
           K     +G  EVD++   +                    K+F+                 
Sbjct: 192 KGLLSSDGTCEVDVWPFLQNLTCDVISRTAFGSSYAEGAKIFELLKRQGYALMTARYARI 251

Query: 252 -------SKDAREGDELARKMQSSIISIIKNREEKVISGEAENYGNDFLALLLKA---KN 311
                  S   R   E+ R ++ S+  II+ RE+ + SG++ +  +D L +LL++   +N
Sbjct: 252 PLWWLLPSTTKRRMKEIERGIRDSLEGIIRKREKALKSGKSTD--DDLLGILLQSNHIEN 311

Query: 312 NSDQRQR---ISVEDVVDECKAMYFSGQETTGVLLSWTAFLLATHVHWQEEARKEVLDMF 371
             D+  +   ++ ++V++ECK  Y +GQETT  LL+WT  LL  H  WQ  AR+EVL +F
Sbjct: 312 KGDENSKSAGMTTQEVMEECKLFYLAGQETTAALLAWTMVLLGKHPEWQARARQEVLQVF 371

Query: 372 GNDNPTLEGITKLKIMSMIIKETLRLYPPTQVMSRLVKKGVRLGKLVLPANIQVSISTIA 431
           GN NP  EG+ +LKI++MI+ E LRLYPP   ++R ++K ++LG L+LPA +QVS+  + 
Sbjct: 372 GNQNPNFEGLGRLKIVTMILYEVLRLYPPGIYLTRALRKDLKLGNLLLPAGVQVSVPILL 431

Query: 432 VHHDTAIWGEDAHEFKPERFSEGKAEDIPSG-AYIPFGLGPRNCVGNMLAINETKITLSM 459
           +HHD  IWG DA EF PERF+EG A+       Y PFG GPR CVG   A+ E KI LS+
Sbjct: 432 IHHDEGIWGNDAKEFNPERFAEGIAKATKGQVCYFPFGWGPRICVGQNFALLEAKIVLSL 491

[Close]

BLAST of Spg026212 vs. ExPASy Swiss-Prot
Match: O48786 (Cytochrome P450 734A1 OS=Arabidopsis thaliana OX=3702 GN=CYP734A1 PE=2 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 8.1e-74
Identity = 175/517 (33.85%), Postives = 275/517 (53.19%), Query Frame = 0


Query: 11  VLLLGLLCASIFYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKMES 70
           VL+L ++ + +      L   L W+P +I      QGI+GPP  F  GN+ +++ M +++
Sbjct: 13  VLVLSVILSLVIVKGMSL---LWWRPRKIEEHFSKQGIRGPPYHFFIGNVKELVGMMLKA 72

Query: 71  MRTSLDITDHNILPRV---HPHWN-----------GNMAQLIVSDPETIKEILHNQERAI 130
               +  + HNILPRV   + HW            G   +L V+DP+ I+EI    E   
Sbjct: 73  SSHPMPFS-HNILPRVLSFYHHWRKIYGATFLVWFGPTFRLTVADPDLIREIFSKSE-FY 132

Query: 131 FKMTLNEDSRRIFGNGQLTSEGEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVEKW- 190
            K   +   +++ G+G L+ +GEKWA  R+I    FH E LK + P ++     MV+KW 
Sbjct: 133 EKNEAHPLVKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLVPVVLKSVTDMVDKWS 192

Query: 191 -KKQPEGK-EVDLFQEFRV----------------------------------------- 250
            K    G+ EVD+++ F++                                         
Sbjct: 193 DKLSENGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFI 252

Query: 251 ---KLFKSKDAREGDELARKMQSSIISIIKNREEKVISGEAEN----YGNDFLALLLKAK 310
              + F ++   +  +L ++++ S++ +I+ R +  I GE E        D L L+++AK
Sbjct: 253 PGYRFFPTRGNLKSWKLDKEIRKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMIQAK 312

Query: 311 NNSDQRQRISVEDVVDECKAMYFSGQETTGVLLSWTAFLLATHVHWQEEARKEVLDMFGN 370
           N       ++V+D+V+ECK+ +F+G++TT  LL+WT  LL+ H  WQ +AR EVL + G+
Sbjct: 313 N-------VTVQDIVEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLRVCGS 372

Query: 371 -DNPTLEGITKLKIMSMIIKETLRLYPPTQVMSRLVKKGVRLGKLVLPANIQVSISTIAV 430
            D PT + + KLK +SMI+ E+LRLYPP     R  K  V+LG   +P   ++ I  IAV
Sbjct: 373 RDVPTKDHVVKLKTLSMILNESLRLYPPIVATIRRAKSDVKLGGYKIPCGTELLIPIIAV 432

Query: 431 HHDTAIWGEDAHEFKPERFSEG--KAEDIPSGAYIPFGLGPRNCVGNMLAINETKITLSM 460
           HHD AIWG D +EF P RF++G  +A   P G +IPFGLG R C+G  LAI + K+TL++
Sbjct: 433 HHDQAIWGNDVNEFNPARFADGVPRAAKHPVG-FIPFGLGVRTCIGQNLAILQAKLTLAV 492

[Close]

BLAST of Spg026212 vs. ExPASy Swiss-Prot
Match: Q9LUC6 (Cytochrome P450 72A14 OS=Arabidopsis thaliana OX=3702 GN=CYP72A14 PE=2 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 1.8e-73
Identity = 171/497 (34.41%), Postives = 253/497 (50.91%), Query Frame = 0


Query: 30  LKLCW-KPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKMESMRTSLDITDHNILPRVHP 89
           LK  W  P  + R +R QG+ G   + + G+  +++ M +E+    +  TD +I PRV P
Sbjct: 25  LKWVWFTPKMLERSLRRQGLSGTSYTPLIGDFKKMISMFIEATSKPIKPTD-DITPRVMP 84

Query: 90  H--------------WNGNMAQLIVSDPETIKEILHN----QERAIFKMTLNEDSRRIFG 149
           H              W G +  + + DPE IKE+ +     Q+   F ++      +I G
Sbjct: 85  HPLQMLKTHGRTNLTWFGPIPTITIMDPEQIKEVFNKVYDFQKAHTFPLS------KILG 144

Query: 150 NGQLTSEGEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGK----EVDL 209
            G ++ +G+KWA+ RRI + AFH E +K M          +V +W K    K    EVD+
Sbjct: 145 TGLVSYDGDKWAQHRRIINPAFHLEKIKNMVHVFHESCSELVGEWDKLVSDKGSSCEVDV 204

Query: 210 -----------------------------------------FQEFRVK---LFKSKDARE 269
                                                    FQ+F +       +K  R 
Sbjct: 205 WPGLTSMTADVISRTAFGSSYREGHRIFELQAELAQLVMQAFQKFFIPGYIYLPTKGNRR 264

Query: 270 GDELARKMQSSIISIIKNREEKVISGEAENYGNDFLALLLKAKNNSDQRQRISVEDVVDE 329
               AR++Q  +  II  RE    SGEA +   D L +LL++     +   +S ED+++E
Sbjct: 265 MKTAAREIQDILRGIINKRERARESGEAPS--EDLLGILLESNLGQTEGNGMSTEDMMEE 324

Query: 330 CKAMYFSGQETTGVLLSWTAFLLATHVHWQEEARKEVLDMFGNDNPTLEGITKLKIMSMI 389
           CK  Y +GQETT VLL WT  LL+ H  WQ  AR+EV  +FG+  P  EG+ +LK+M+MI
Sbjct: 325 CKLFYLAGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKQPDTEGLNQLKVMTMI 384

Query: 390 IKETLRLYPPTQVMSRLVKKGVRLGKLVLPANIQVSISTIAVHHDTAIWGEDAHEFKPER 449
           + E LRLYPP   ++R + K ++LG L LP  +Q+S+  + VH DT +WG DA EFKPER
Sbjct: 385 LYEVLRLYPPVVQLTRAIHKEMKLGDLTLPGGVQISLPVLLVHRDTELWGNDAGEFKPER 444

Query: 450 FSEGKAEDIPSG-AYIPFGLGPRNCVGNMLAINETKITLSMILQRFSFTISPGHVHAPYQ 459
           F +G ++   +  ++ PF  GPR C+G    + E K+ +S+ILQRFSF +SP +VHAPY 
Sbjct: 445 FKDGLSKATKNQVSFFPFAWGPRICIGQNFTLLEAKMAMSLILQRFSFELSPSYVHAPYT 504

[Close]

BLAST of Spg026212 vs. ExPASy TrEMBL
Match: A0A6J1D5N2 (cytochrome P450 CYP749A22-like OS=Momordica charantia OX=3673 GN=LOC111017532 PE=3 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 7.3e-195
Identity = 357/511 (69.86%), Postives = 399/511 (78.08%), Query Frame = 0


Query: 9   TTVLLLGLLCA-SIFYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMK 68
           T V LLG+L A SIFY    + LKL WKP RI+R MRSQG+ GPPS+FIYGN+ Q+MKMK
Sbjct: 5   TAVFLLGVLSASSIFY----VLLKLWWKPLRIQRIMRSQGVYGPPSNFIYGNMIQVMKMK 64

Query: 69  MESMRTSLDITDHNILPRVHPH--------------WNGNMAQLIVSDPETIKEILHNQE 128
            ESMRTSLDI+DH+ILPRV+PH              WNG  AQLIVSDPETIKEILHN+E
Sbjct: 65  FESMRTSLDISDHHILPRVNPHIHLWIDKYGKNFIQWNGTQAQLIVSDPETIKEILHNRE 124

Query: 129 RAIFKMTLNEDSRRIFGNGQLTSEGEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVE 188
           RAI K+ L  D+RRIFG+G  TS+GEKWAK RRIADYAFHGELLK M PTMVSCAEAMVE
Sbjct: 125 RAITKIDLGGDARRIFGDGLSTSDGEKWAKARRIADYAFHGELLKNMMPTMVSCAEAMVE 184

Query: 189 KWKKQPEGKEVDLFQEFRV----------------------------------------- 248
           KWKK  EGKE+DLF+EF++                                         
Sbjct: 185 KWKKNDEGKELDLFEEFKLLTSDIIAHTAFGSSYLEGKFIFETLSKLSMILFKNQFKRRI 244

Query: 249 ----KLFKSKDAREGDELARKMQSSIISIIKNREEKVISGEAENYGNDFLALLLKAKNNS 308
               KLFK+KDAREGDEL  +M++SI+SIIK REEKV+SGEA+NYGNDFL LLLKAKN+ 
Sbjct: 245 PGFSKLFKTKDAREGDELEMRMKNSIVSIIKKREEKVMSGEADNYGNDFLGLLLKAKNDP 304

Query: 309 DQRQRISVEDVVDECKAMYFSGQETTGVLLSWTAFLLATHVHWQEEARKEVLDMFGNDNP 368
           D RQRISV+DVVDECK +YF+GQETT VLLSWT+FLLATHV WQEEARKEV DMFGN NP
Sbjct: 305 DPRQRISVDDVVDECKTVYFAGQETTNVLLSWTSFLLATHVDWQEEARKEVFDMFGNTNP 364

Query: 369 TLEGITKLKIMSMIIKETLRLYPPTQVMSRLVKKGVRLGKLVLPANIQVSISTIAVHHDT 428
           TLEGI KLKIMSMIIKE+LRLYPP   MSR+VKK VRLGKLVLPA+IQVSIS IAVHHDT
Sbjct: 365 TLEGIAKLKIMSMIIKESLRLYPPATPMSRMVKKEVRLGKLVLPASIQVSISNIAVHHDT 424

Query: 429 AIWGEDAHEFKPERFSEGKAEDIPSGAYIPFGLGPRNCVGNMLAINETKITLSMILQRFS 460
           A WGEDAHEFKPERFSEG A+DIPS AYIPFGLGPRNCVGN+LA+NETKI LSMILQRFS
Sbjct: 425 ATWGEDAHEFKPERFSEGTAKDIPSAAYIPFGLGPRNCVGNILAVNETKIALSMILQRFS 484

[Close]

BLAST of Spg026212 vs. ExPASy TrEMBL
Match: A0A1S3BF46 (cytochrome P450 CYP749A22-like OS=Cucumis melo OX=3656 GN=LOC103489195 PE=3 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 1.2e-133
Identity = 252/503 (50.10%), Postives = 326/503 (64.81%), Query Frame = 0


Query: 17  LCASIFYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKMESMRTSLD 76
           L   + ++ FKLF+KL W P RI+RFMRSQGIQGP   FI GN   +   +M++M T +D
Sbjct: 13  LSGCLLFVVFKLFVKLWWTPMRIQRFMRSQGIQGPSYKFIQGNTRDVYMKRMQAMATPMD 72

Query: 77  ITDHNILPRVHP--------------HWNGNMAQLIVSDPETIKEILHNQERAIFKMTLN 136
           ++ HNILPRV P               W G  AQL+++DPE IKE+LH+++++  K  L 
Sbjct: 73  LS-HNILPRVMPSVHSWLNLYGRNYLQWAGVDAQLMITDPEMIKEVLHDRQKSFPKAKLK 132

Query: 137 EDSRRIFGNGQLTSEGEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGK 196
               RIFGNG  T+EG++W   RRIA++AFHG+ LK M PTM+ C E M+E WK   EGK
Sbjct: 133 GHIDRIFGNGLATAEGQRWVNSRRIANFAFHGDSLKNMIPTMIECGEKMIEGWKNY-EGK 192

Query: 197 EVDLFQEFRV---------------------------------------------KLFKS 256
           E+D F+EF+V                                             K+ KS
Sbjct: 193 ELDAFKEFKVFTLDVISHTAFGSSYQQGKKIFHMLQELCDLSIRNGYKIKLPIISKILKS 252

Query: 257 KDAREGDELARKMQSSIISIIKNREEKVISGEAENYGNDFLALLLKAKNNSDQRQRISVE 316
           KD  EG+ L ++++   + IIK REEK+ + EA +YGNDFL LL+KAKN+ +  Q IS+E
Sbjct: 253 KDDYEGERLEKRLKDCFMEIIKEREEKLRNDEASDYGNDFLGLLIKAKNDPETSQSISME 312

Query: 317 DVVDECKAMYFSGQETTGVLLSWTAFLLATHVHWQEEARKEVLDMFGNDNPTLEGITKLK 376
           D+VDECK  YF+G ETT VLL+WT  LLA H  WQE+AR EV D+FG+++PTLEG+ KLK
Sbjct: 313 DIVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEVFDVFGHNHPTLEGLPKLK 372

Query: 377 IMSMIIKETLRLYPPTQVMSRLVKKGVRLGKLVLPANIQVSISTIAVHHDTAIWGEDAHE 436
            M+MII E LRLYPP   ++R V+K VRLG LV+P    ++I T+AVHHDT  WG+DAHE
Sbjct: 373 AMAMIINECLRLYPPAMTIARRVEKEVRLGNLVVPTTTLLAIPTVAVHHDTKFWGDDAHE 432

Query: 437 FKPERFSE--GKAEDIPSGAYIPFGLGPRNCVGNMLAINETKITLSMILQRFSFTISPGH 459
           FKPERFSE  GKA +  S AY+PFGLGPRNCVG   A NE KI +SMILQR+SF++SP +
Sbjct: 433 FKPERFSEGIGKATEKNSAAYLPFGLGPRNCVGMNFATNEAKIAMSMILQRYSFSLSPAY 492

[Close]

BLAST of Spg026212 vs. ExPASy TrEMBL
Match: A0A6J1D6R8 (cytochrome P450 CYP749A22-like OS=Momordica charantia OX=3673 GN=LOC111017564 PE=3 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 1.4e-132
Identity = 251/495 (50.71%), Postives = 330/495 (66.67%), Query Frame = 0


Query: 27  KLFLKLCWKPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKMESMRTSLDITDHNILPRV 86
           KLF+KL W P RI+RFMRSQGIQGP  +FI GN  ++   +  ++ T +D++ H ILPRV
Sbjct: 23  KLFIKLWWTPIRIQRFMRSQGIQGPSYNFIQGNAREMHTRRTHAIATPIDLS-HRILPRV 82

Query: 87  HPH--------------WNGNMAQLIVSDPETIKEILHNQERAIFKMTLNEDSRRIFGNG 146
            PH              W G  AQLI++DPE I+E+LH++++   K  L+    +IFGNG
Sbjct: 83  LPHTHSWLHRYGRNFIQWFGVEAQLIITDPEMIREVLHDRQKKFPKAKLHGFLNKIFGNG 142

Query: 147 QLTSEGEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGKEVDLFQEFRV 206
            +T+EGE WAK R+IA++AFHG+ LK M P M+ CAE M+E+WK   EGKE+D+FQ  +V
Sbjct: 143 LVTAEGETWAKSRKIANFAFHGDTLKNMIPAMIGCAETMMEEWKHY-EGKELDVFQHLKV 202

Query: 207 ---------------------------------------------KLFKSKDAREGDELA 266
                                                        K+FKSKD  EG+ L 
Sbjct: 203 YTSDVISHTAFGSSYEQGKNIFQMLQNLCELIIRNGFKIRLPGIRKIFKSKDDVEGERLE 262

Query: 267 RKMQSSIISIIKNREEKVISGEAENYGNDFLALLLKAKNNSDQRQRISVEDVVDECKAMY 326
           ++M+   + +IK+REEK I+GEA+ YGNDFL LL+KAKN+ ++ QRISV+D+VDECK  Y
Sbjct: 263 KRMKDCFMEVIKSREEKFINGEADGYGNDFLGLLVKAKNDPEESQRISVDDIVDECKTFY 322

Query: 327 FSGQETTGVLLSWTAFLLATHVHWQEEARKEVLDMFGNDNPTLEGITKLKIMSMIIKETL 386
           F+G ETT VLL+WT FLLA H  WQ++AR EV  +FG+ NPT EG+ KLK M+MI+ E L
Sbjct: 323 FAGHETTSVLLAWTMFLLALHKQWQDQARDEVFKVFGDKNPTSEGLPKLKTMTMILYECL 382

Query: 387 RLYPPTQVMSR-LVKKGVRLGKLVLPANIQVSISTIAVHHDTAIWGEDAHEFKPERFSEG 446
           RLYPP   + R +++K VRLG LVLPA I+V+I T AVHHDTA WGEDA EFKPERF+EG
Sbjct: 383 RLYPPAMTVGRQVMEKEVRLGSLVLPATIRVTIPTAAVHHDTAYWGEDASEFKPERFAEG 442

Query: 447 KAEDIP--SGAYIPFGLGPRNCVGNMLAINETKITLSMILQRFSFTISPGHVHAPYQFLT 460
            A+ I   S AY+PFGLG R+CVG   A+ E KI +SMILQR+SFT+SP + H+P QFLT
Sbjct: 443 VAKAIERNSAAYLPFGLGGRSCVGMNFAMKEAKIGMSMILQRYSFTLSPAYAHSPVQFLT 502

[Close]

BLAST of Spg026212 vs. ExPASy TrEMBL
Match: A0A6J1G0I3 (cytochrome P450 CYP749A22-like OS=Cucurbita moschata OX=3662 GN=LOC111449593 PE=3 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 3.7e-130
Identity = 246/516 (47.67%), Postives = 331/516 (64.15%), Query Frame = 0


Query: 5   GFSDTTVLLLGLLCASIFYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSSFIYGNLFQIM 64
           G+   + +L  ++C  I     K+  KL WKP RI+R MR+QGI+GP  +FI GN+ ++ 
Sbjct: 6   GYIIPSFVLGCVVCGGI----IKVVAKLWWKPMRIQRMMRAQGIEGPSYNFIQGNMAEMY 65

Query: 65  KMKMESMRTSLDITDHNILPRVHPH--------------WNGNMAQLIVSDPETIKEILH 124
             +M +M T +D++ HNILPRV PH              W G  AQL+++DPE IKE+ H
Sbjct: 66  TKRMHAMATPMDLS-HNILPRVLPHVYSWLNHYGKTFLQWYGMEAQLVITDPEMIKEVFH 125

Query: 125 NQERAIFKMTLNEDSRRIFGNGQLTSEGEKWAKIRRIADYAFHGELLKKMGPTMVSCAEA 184
           ++++   K  L++   RIFGNG +TSEGE+WAK R+IA+YAFHG+ LK M P M+ CA  
Sbjct: 126 DRQKNYPKAKLDDHLLRIFGNGLVTSEGERWAKSRKIANYAFHGDSLKNMIPAMIECAAT 185

Query: 185 MVEKWKKQPEGKEVDLFQEFRV-------------------------------------- 244
           M+E+WK   EGKE+D+ +E +V                                      
Sbjct: 186 MIERWKLF-EGKELDVLKELKVYTLDVISHTAFGSSYEQGKSIFQMLQDLSDLSIRNGYK 245

Query: 245 -------KLFKSKDAREGDELARKMQSSIISIIKNREEKVISGEAENYGNDFLALLLKAK 304
                  K+FKSKD  EG+ L ++M+   + +IK+REEK ++GEAE YGNDFL LL+KAK
Sbjct: 246 IRVPVISKIFKSKDDVEGERLEKRMEEWFMEMIKSREEKFMNGEAEGYGNDFLGLLVKAK 305

Query: 305 NNSDQRQRISVEDVVDECKAMYFSGQETTGVLLSWTAFLLATHVHWQEEARKEVLDMFGN 364
           N+ +   RIS + +V ECK  YF+G ETT VL++WT FLLA H  WQ+EAR EVL++FG 
Sbjct: 306 NDPENSHRISADVIVAECKTFYFAGHETTNVLIAWTMFLLAMHKEWQDEARNEVLEIFGT 365

Query: 365 DNPTLEGITKLKIMSMIIKETLRLYPPTQVMSRLVKKGVRLGKLVLPANIQVSISTIAVH 424
            NP+ EG+ KL+ M+MII E LRLYPP   +SR V+K VRLG+L LPA +Q+++ TIAVH
Sbjct: 366 KNPSSEGLPKLRTMTMIINECLRLYPPAMTVSRQVEKEVRLGRLRLPATLQLTVPTIAVH 425

Query: 425 HDTAIWGEDAHEFKPERFSE--GKAEDIPSGAYIPFGLGPRNCVGNMLAINETKITLSMI 460
           HD A WGEDAHEFKPERF E  GK  +  S  Y+PFGLGPR+CVG   A+NE KI +SMI
Sbjct: 426 HDKAFWGEDAHEFKPERFGEGVGKVMERNSAGYLPFGLGPRSCVGMSFAMNEAKIAMSMI 485

[Close]

BLAST of Spg026212 vs. ExPASy TrEMBL
Match: A0A5D3CAL6 (Cytochrome P450 CYP749A22-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G00230 PE=4 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 6.3e-130
Identity = 249/503 (49.50%), Postives = 321/503 (63.82%), Query Frame = 0


Query: 27  KLFLKLCWKPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKMESMRTSLDITDHNILPRV 86
           +LF+KL W P RI+RFMRSQGIQGP   FI GN   +   +M++M T +D++ HNILPRV
Sbjct: 427 QLFVKLWWTPMRIQRFMRSQGIQGPSYKFIQGNTRDVYMKRMQAMATPMDLS-HNILPRV 486

Query: 87  HP--------------HWNGNMAQLIVSDPETIKEILHNQERAIFKMTLNEDSRRIFGNG 146
            P               W G  AQL+++DPE IKE+LH+++++  K  L     RIFGNG
Sbjct: 487 MPSVHSWLNLYGRNYLQWAGVDAQLMITDPEMIKEVLHDRQKSFPKAKLKGHIDRIFGNG 546

Query: 147 QLTSEGEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGKEVDLFQEFRV 206
             T+EG++W   RRIA++AFHG+ LK M PTM+ C E M+E WK   EGKE+D F+EF+V
Sbjct: 547 LATAEGQRWVNSRRIANFAFHGDSLKNMIPTMIECGEKMIEGWKNY-EGKELDAFKEFKV 606

Query: 207 ---------------------------------------------KLFKSKDAREGDELA 266
                                                        K+ KSKD  EG+ L 
Sbjct: 607 FTLDVISHTAFGSSYQQGKKIFHMLQELCDLSIRNGYKIKLPIISKILKSKDDYEGERLE 666

Query: 267 RKMQSSIISIIKNREEKVISGEAENYGNDFLALLLKAKNNSDQRQRISVEDVVDECKAMY 326
           ++++   + IIK REEK+ + EA +YGNDFL LL+KAKN+ +  Q IS+ED+VDECK  Y
Sbjct: 667 KRLKDCFMEIIKEREEKLRNDEASDYGNDFLGLLIKAKNDPETSQSISMEDIVDECKTFY 726

Query: 327 FSGQETTGVLLSWTAFLLATHVHWQEEARKEVLDMFGNDNPTLEGITKLKI--------- 386
           F+G ETT VLL+WT  LLA H  WQE+AR EV D+FG+++PTLEG+ KLK          
Sbjct: 727 FAGHETTNVLLAWTMLLLALHKEWQEKARNEVFDVFGHNHPTLEGLPKLKADVCVRFYYG 786

Query: 387 -MSMIIKETLRLYPPTQVMSRLVKKGVRLGKLVLPANIQVSISTIAVHHDTAIWGEDAHE 446
            M+MII E LRLYPP   ++R V+K VRLG LV+P    ++I T+AVHHDT  WG+DAHE
Sbjct: 787 QMAMIINECLRLYPPAMTIARRVEKEVRLGNLVVPTTTLLAIPTVAVHHDTKFWGDDAHE 846

Query: 447 FKPERFSE--GKAEDIPSGAYIPFGLGPRNCVGNMLAINETKITLSMILQRFSFTISPGH 459
           FKPERFSE  GKA +  S AY+PFGLGPRNCVG   A NE KI +SMILQR+SF++SP +
Sbjct: 847 FKPERFSEGIGKATEKNSAAYLPFGLGPRNCVGMNFATNEAKIAMSMILQRYSFSLSPAY 906

[Close]

BLAST of Spg026212 vs. TAIR 10
Match: AT2G26710.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 279.3 bits (713), Expect = 5.7e-75
Identity = 175/517 (33.85%), Postives = 275/517 (53.19%), Query Frame = 0


Query: 11  VLLLGLLCASIFYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKMES 70
           VL+L ++ + +      L   L W+P +I      QGI+GPP  F  GN+ +++ M +++
Sbjct: 13  VLVLSVILSLVIVKGMSL---LWWRPRKIEEHFSKQGIRGPPYHFFIGNVKELVGMMLKA 72

Query: 71  MRTSLDITDHNILPRV---HPHWN-----------GNMAQLIVSDPETIKEILHNQERAI 130
               +  + HNILPRV   + HW            G   +L V+DP+ I+EI    E   
Sbjct: 73  SSHPMPFS-HNILPRVLSFYHHWRKIYGATFLVWFGPTFRLTVADPDLIREIFSKSE-FY 132

Query: 131 FKMTLNEDSRRIFGNGQLTSEGEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVEKW- 190
            K   +   +++ G+G L+ +GEKWA  R+I    FH E LK + P ++     MV+KW 
Sbjct: 133 EKNEAHPLVKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLVPVVLKSVTDMVDKWS 192

Query: 191 -KKQPEGK-EVDLFQEFRV----------------------------------------- 250
            K    G+ EVD+++ F++                                         
Sbjct: 193 DKLSENGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFI 252

Query: 251 ---KLFKSKDAREGDELARKMQSSIISIIKNREEKVISGEAEN----YGNDFLALLLKAK 310
              + F ++   +  +L ++++ S++ +I+ R +  I GE E        D L L+++AK
Sbjct: 253 PGYRFFPTRGNLKSWKLDKEIRKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMIQAK 312

Query: 311 NNSDQRQRISVEDVVDECKAMYFSGQETTGVLLSWTAFLLATHVHWQEEARKEVLDMFGN 370
           N       ++V+D+V+ECK+ +F+G++TT  LL+WT  LL+ H  WQ +AR EVL + G+
Sbjct: 313 N-------VTVQDIVEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLRVCGS 372

Query: 371 -DNPTLEGITKLKIMSMIIKETLRLYPPTQVMSRLVKKGVRLGKLVLPANIQVSISTIAV 430
            D PT + + KLK +SMI+ E+LRLYPP     R  K  V+LG   +P   ++ I  IAV
Sbjct: 373 RDVPTKDHVVKLKTLSMILNESLRLYPPIVATIRRAKSDVKLGGYKIPCGTELLIPIIAV 432

Query: 431 HHDTAIWGEDAHEFKPERFSEG--KAEDIPSGAYIPFGLGPRNCVGNMLAINETKITLSM 460
           HHD AIWG D +EF P RF++G  +A   P G +IPFGLG R C+G  LAI + K+TL++
Sbjct: 433 HHDQAIWGNDVNEFNPARFADGVPRAAKHPVG-FIPFGLGVRTCIGQNLAILQAKLTLAV 492

[Close]

BLAST of Spg026212 vs. TAIR 10
Match: AT3G14680.1 (cytochrome P450, family 72, subfamily A, polypeptide 14 )

HSP 1 Score: 278.1 bits (710), Expect = 1.3e-74
Identity = 171/497 (34.41%), Postives = 253/497 (50.91%), Query Frame = 0


Query: 30  LKLCW-KPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKMESMRTSLDITDHNILPRVHP 89
           LK  W  P  + R +R QG+ G   + + G+  +++ M +E+    +  TD +I PRV P
Sbjct: 25  LKWVWFTPKMLERSLRRQGLSGTSYTPLIGDFKKMISMFIEATSKPIKPTD-DITPRVMP 84

Query: 90  H--------------WNGNMAQLIVSDPETIKEILHN----QERAIFKMTLNEDSRRIFG 149
           H              W G +  + + DPE IKE+ +     Q+   F ++      +I G
Sbjct: 85  HPLQMLKTHGRTNLTWFGPIPTITIMDPEQIKEVFNKVYDFQKAHTFPLS------KILG 144

Query: 150 NGQLTSEGEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGK----EVDL 209
            G ++ +G+KWA+ RRI + AFH E +K M          +V +W K    K    EVD+
Sbjct: 145 TGLVSYDGDKWAQHRRIINPAFHLEKIKNMVHVFHESCSELVGEWDKLVSDKGSSCEVDV 204

Query: 210 -----------------------------------------FQEFRVK---LFKSKDARE 269
                                                    FQ+F +       +K  R 
Sbjct: 205 WPGLTSMTADVISRTAFGSSYREGHRIFELQAELAQLVMQAFQKFFIPGYIYLPTKGNRR 264

Query: 270 GDELARKMQSSIISIIKNREEKVISGEAENYGNDFLALLLKAKNNSDQRQRISVEDVVDE 329
               AR++Q  +  II  RE    SGEA +   D L +LL++     +   +S ED+++E
Sbjct: 265 MKTAAREIQDILRGIINKRERARESGEAPS--EDLLGILLESNLGQTEGNGMSTEDMMEE 324

Query: 330 CKAMYFSGQETTGVLLSWTAFLLATHVHWQEEARKEVLDMFGNDNPTLEGITKLKIMSMI 389
           CK  Y +GQETT VLL WT  LL+ H  WQ  AR+EV  +FG+  P  EG+ +LK+M+MI
Sbjct: 325 CKLFYLAGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKQPDTEGLNQLKVMTMI 384

Query: 390 IKETLRLYPPTQVMSRLVKKGVRLGKLVLPANIQVSISTIAVHHDTAIWGEDAHEFKPER 449
           + E LRLYPP   ++R + K ++LG L LP  +Q+S+  + VH DT +WG DA EFKPER
Sbjct: 385 LYEVLRLYPPVVQLTRAIHKEMKLGDLTLPGGVQISLPVLLVHRDTELWGNDAGEFKPER 444

Query: 450 FSEGKAEDIPSG-AYIPFGLGPRNCVGNMLAINETKITLSMILQRFSFTISPGHVHAPYQ 459
           F +G ++   +  ++ PF  GPR C+G    + E K+ +S+ILQRFSF +SP +VHAPY 
Sbjct: 445 FKDGLSKATKNQVSFFPFAWGPRICIGQNFTLLEAKMAMSLILQRFSFELSPSYVHAPYT 504

[Close]

BLAST of Spg026212 vs. TAIR 10
Match: AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2 )

HSP 1 Score: 275.4 bits (703), Expect = 8.3e-74
Identity = 160/483 (33.13%), Postives = 249/483 (51.55%), Query Frame = 0


Query: 36  PFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKMESMRTSLDITDHNILPRVHPH------ 95
           P RI++FM  QGI GP    + GN+  I KM   S         HNI+PR+ PH      
Sbjct: 33  PRRIKKFMERQGITGPKPRLLTGNIIDISKMLSHSASNDCSSIHHNIVPRLLPHYVSWSK 92

Query: 96  --------WNGNMAQLIVSDPETIKEILHNQERAIFKMTLNEDSRRIF-GNGQLTSEGEK 155
                   WNG   +L +++ E IKE+L        K  L +   + F G G L + GE 
Sbjct: 93  QYGKRFIMWNGTEPRLCLTETEMIKELLTKHNPVTGKSWLQQQGTKGFIGRGLLMANGEA 152

Query: 156 WAKIRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGKEVDLFQEFR--------- 215
           W   R +A  AF  + LK     MV C + M E+ +K+  G+EV++ +E R         
Sbjct: 153 WHHQRHMAAPAFTRDRLKGYAKHMVECTKMMAERLRKE-VGEEVEIGEEMRRLTADIISR 212

Query: 216 -----------------------------------VKLFKSKDAREGDELARKMQSSIIS 275
                                               +   SK  RE   L  +++  ++ 
Sbjct: 213 TEFGSSCDKGKELFSLLTVLQRLCAQATRHLCFPGSRFLPSKYNREIKSLKTEVERLLME 272

Query: 276 IIKNREEKVISGEAENYGNDFLALLLKAKNNSDQRQRISVEDVVDECKAMYFSGQETTGV 335
           II +R++ V  G + +YG+D L LLL   +++  +  ++V+ ++DECK  +F+G ETT +
Sbjct: 273 IIDSRKDSVEIGRSSSYGDDLLGLLLNQMDSN--KNNLNVQMIMDECKTFFFTGHETTSL 332

Query: 336 LLSWTAFLLATHVHWQEEARKEVLDMFGNDN-PTLEGITKLKIMSMIIKETLRLYPPTQV 395
           LL+WT  LLA +  WQ+  R EV  + G D  P++E ++ L  ++ +I E+LRLYPP  +
Sbjct: 333 LLTWTLMLLAHNPTWQDNVRDEVRQVCGQDGVPSVEQLSSLTSLNKVINESLRLYPPATL 392

Query: 396 MSRLVKKGVRLGKLVLPANIQVSISTIAVHHDTAIWGEDAHEFKPERFSEGKAEDIPSGA 455
           + R+  + ++LG L++P  + + I  +A+HH   +WGEDA+EF PERF+        S  
Sbjct: 393 LPRMAFEDIKLGDLIIPKGLSIWIPVLAIHHSNELWGEDANEFNPERFTTRSFAS--SRH 452

Query: 456 YIPFGLGPRNCVGNMLAINETKITLSMILQRFSFTISPGHVHAPYQFLTNCPQHGVRVKL 459
           ++PF  GPRNC+G   A+ E KI L+M++ +FSF IS  + HAP   LT  P++GV++ L
Sbjct: 453 FMPFAAGPRNCIGQTFAMMEAKIILAMLVSKFSFAISENYRHAPIVVLTIKPKYGVQLVL 510

[Close]

BLAST of Spg026212 vs. TAIR 10
Match: AT2G46950.1 (cytochrome P450, family 709, subfamily B, polypeptide 2 )

HSP 1 Score: 275.0 bits (702), Expect = 1.1e-73
Identity = 168/494 (34.01%), Postives = 256/494 (51.82%), Query Frame = 0


Query: 32  LCWKPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKMKMESMRTSLDITDHNILPRVHPH-- 91
           L W+P+ + R  + QGI GP    +YGNL +I KMK E+    LD   ++I+PRV PH  
Sbjct: 84  LVWRPWMLSRRFKKQGISGPKYRILYGNLREIRKMKNEAKLMVLDPNSNDIVPRVLPHLQ 143

Query: 92  ------------WNGNMAQLIVSDPETIKEILHNQERAIFKMTLNEDSRRIFGNGQLTSE 151
                       W G   +L +SD E  K+IL N+     K     +  ++ GNG +   
Sbjct: 144 QWKSQYGETFLYWQGTDPRLCISDHELAKQILSNKFVFFSKSKTKPEILKLSGNGLIFVN 203

Query: 152 GEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGKEVDLF----QEFR-- 211
           G  W + RRI + AF  + LK M   MV C   M  +WKKQ  G E + F    +EF+  
Sbjct: 204 GLDWVRHRRILNPAFSMDKLKLMTQLMVDCTFRMFLEWKKQRNGVETEQFVLISREFKRL 263

Query: 212 -----------------VKLFKSK-------DAREGD------------------ELARK 271
                            +++FKS+        A   D                  +L  K
Sbjct: 264 TADIIATAAFGSSYAEGIEVFKSQLELQKCCAAALTDLYFPGIQYLPTPSNLQIWKLDMK 323

Query: 272 MQSSIISIIKNREEKVISGEAENYGNDFLALLLKAKNNSDQRQRISVEDVVDECKAMYFS 331
           + SSI  II  R    ++ E+++YGND L ++L A ++++  +++S++++++ECK  +F+
Sbjct: 324 VNSSIKRIIDAR----LTSESKDYGNDLLGIMLTAASSNESEKKMSIDEIIEECKTFFFA 383

Query: 332 GQETTGVLLSWTAFLLATHVHWQEEARKEVLDMFGNDN-PTLEGITKLKIMSMIIKETLR 391
           G ETT  LL+W+  LL+ H  WQE+ R+EV +  G D  P  E  +KLK+M+ +  E+LR
Sbjct: 384 GHETTANLLTWSTMLLSLHQDWQEKLREEVFNECGKDKIPDAETCSKLKLMNTVFMESLR 443

Query: 392 LYPPTQVMSRLVKKGVRLGKLVLPANIQVSISTIAVHHDTAIWGEDAHEFKPERFSEG-- 451
           LY P   + RL  + ++LG L +P    + +    +H D A+WG DA +F P RF+ G  
Sbjct: 444 LYGPVLNLLRLASEDMKLGNLEIPKGTTIILPIAKMHRDKAVWGSDADKFNPMRFANGLS 503

Query: 452 KAEDIPSGAYIPFGLGPRNCVGNMLAINETKITLSMILQRFSFTISPGHVHAPYQFLTNC 461
           +A + P+ A + F +GPR C+G   AI E K  L+MILQRF   +S  + HAP   LT  
Sbjct: 504 RAANHPN-ALLAFSMGPRACIGQNFAIMEAKTVLAMILQRFRLNLSADYKHAPADHLTLQ 563

[Close]

BLAST of Spg026212 vs. TAIR 10
Match: AT3G14610.1 (cytochrome P450, family 72, subfamily A, polypeptide 7 )

HSP 1 Score: 273.1 bits (697), Expect = 4.1e-73
Identity = 169/516 (32.75%), Postives = 268/516 (51.94%), Query Frame = 0


Query: 7   SDTTVLLLGLLCASIFYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSSFIYGNLFQIMKM 66
           S + V  L +L A +    +++   +  KP  +   ++ QG+ G P + + G++ + + M
Sbjct: 2   SFSVVAALPVLVAVVVLWTWRIVKWVWIKPKMLESSLKRQGLTGTPYTPLVGDIKRNVDM 61

Query: 67  KMESMRTSLDITDHNILPRVHP--------------HWNGNMAQLIVSDPETIKEILHNQ 126
            ME+    +++TD +I PR+ P               W G +  +++++PE IKE+ +  
Sbjct: 62  MMEARSKPINVTD-DITPRLLPLALKMLNSHGKTFFIWIGPLPTIVITNPEQIKEVFNKV 121

Query: 127 ERAIFKMTLNEDSRRIFGNGQLTSEGEKWAKIRRIADYAFHGELLKKMGPTMVSCAEAMV 186
               F+        R+   G  + +G+KWA  RRI + AFH E +K M P    C   +V
Sbjct: 122 ND--FEKASTFPLIRLLAGGLASYKGDKWASHRRIINPAFHLEKIKNMIPAFYHCCSEVV 181

Query: 187 EKWKK-----------------------------------------QPEGKEVDLF-QEF 246
            +W+K                                         Q +G+  +L  Q F
Sbjct: 182 CQWEKLFTDKESPLEVDVWPWLVNMTADVISHTAFGSSYKEGQRIFQLQGELAELIAQAF 241

Query: 247 R------VKLFKSKDAREGDELARKMQSSIISIIKNREEKVISGEAENYGNDFLALLLKA 306
           +       + + +K  R    + R++   +  I+  RE+   +GE  N  +D L +LL++
Sbjct: 242 KKSYIPGSRFYPTKSNRRMKAIDREVDVILRGIVSKREKAREAGEPAN--DDLLGILLES 301

Query: 307 KNNSDQRQRISVEDVVDECKAMYFSGQETTGVLLSWTAFLLATHVHWQEEARKEVLDMFG 366
            +   Q   +SVEDV+ ECK  YF+GQETT VLL WT  LL+ H  WQ  AR+EV+ + G
Sbjct: 302 NSEESQGNGMSVEDVMKECKLFYFAGQETTSVLLVWTMVLLSHHQDWQARAREEVMQVLG 361

Query: 367 -NDNPTLEGITKLKIMSMIIKETLRLYPPTQVMSRLVKKGVRLGKLVLPANIQVSISTIA 426
            N+ P +E +  LK+M+MI  E LRLYPP   + R+V K ++LG+L LPA IQ+ + TI 
Sbjct: 362 ENNKPDMESLNNLKVMTMIFNEVLRLYPPVAQLKRVVNKEMKLGELTLPAGIQIYLPTIL 421

Query: 427 VHHDTAIWGEDAHEFKPERFSEGKAEDIPSG-AYIPFGLGPRNCVGNMLAINETKITLSM 459
           V  DT +WG+DA +FKPERF +G ++   +  ++ PFG GPR C+G   A+ E K+ +++
Sbjct: 422 VQRDTELWGDDAADFKPERFRDGLSKATKNQVSFFPFGWGPRICIGQNFAMLEAKMAMAL 481

The following BLAST results are available for this feature:

BLAST of Spg026212 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022149008.1	1.5e-194	69.86	cytochrome P450 CYP749A22-like [Momordica charantia]	[more]
XP_038892924.1	7.9e-135	51.79	cytochrome P450 CYP749A22-like isoform X1 [Benincasa hispida]	[more]
XP_038892699.1	5.1e-134	50.99	cytochrome P450 CYP749A22-like [Benincasa hispida] >XP_038892700.1 cytochrome P4...	[more]
XP_004135430.2	1.5e-133	50.89	cytochrome P450 CYP749A22 isoform X1 [Cucumis sativus] >KAE8648770.1 hypothetica...	[more]
XP_008446466.1	2.5e-133	50.10	PREDICTED: cytochrome P450 CYP749A22-like [Cucumis melo]	[more]

BLAST of Spg026212 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
H2DH17	1.2e-114	43.11	Cytochrome P450 CYP749A22 OS=Panax ginseng OX=4054 PE=2 SV=1	[more]
W8JWW3	3.9e-76	34.43	Cytochrome P450 72A225 OS=Catharanthus roseus OX=4058 GN=CYP72A225 PE=2 SV=1	[more]
H1A988	3.3e-75	35.27	11-oxo-beta-amyrin 30-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP72A154 PE=...	[more]
O48786	8.1e-74	33.85	Cytochrome P450 734A1 OS=Arabidopsis thaliana OX=3702 GN=CYP734A1 PE=2 SV=1	[more]
Q9LUC6	1.8e-73	34.41	Cytochrome P450 72A14 OS=Arabidopsis thaliana OX=3702 GN=CYP72A14 PE=2 SV=1	[more]

BLAST of Spg026212 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D5N2	7.3e-195	69.86	cytochrome P450 CYP749A22-like OS=Momordica charantia OX=3673 GN=LOC111017532 PE...	[more]
A0A1S3BF46	1.2e-133	50.10	cytochrome P450 CYP749A22-like OS=Cucumis melo OX=3656 GN=LOC103489195 PE=3 SV=1	[more]
A0A6J1D6R8	1.4e-132	50.71	cytochrome P450 CYP749A22-like OS=Momordica charantia OX=3673 GN=LOC111017564 PE...	[more]
A0A6J1G0I3	3.7e-130	47.67	cytochrome P450 CYP749A22-like OS=Cucurbita moschata OX=3662 GN=LOC111449593 PE=...	[more]
A0A5D3CAL6	6.3e-130	49.50	Cytochrome P450 CYP749A22-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]

BLAST of Spg026212 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G26710.1	5.7e-75	33.85	Cytochrome P450 superfamily protein	[more]
AT3G14680.1	1.3e-74	34.41	cytochrome P450, family 72, subfamily A, polypeptide 14	[more]
AT1G67110.1	8.3e-74	33.13	cytochrome P450, family 735, subfamily A, polypeptide 2	[more]
AT2G46950.1	1.1e-73	34.01	cytochrome P450, family 709, subfamily B, polypeptide 2	[more]
AT3G14610.1	4.1e-73	32.75	cytochrome P450, family 72, subfamily A, polypeptide 7	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 406..417 score: 39.52 coord: 269..286 score: 36.02 coord: 397..406 score: 60.84 coord: 321..332 score: 45.71
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 190..435 e-value: 7.4E-59 score: 199.7 coord: 50..190 e-value: 4.0E-9 score: 35.7
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 406..429 score: 37.07 coord: 258..275 score: 21.53 coord: 278..304 score: 24.36 coord: 361..385 score: 22.68 coord: 396..406 score: 51.62
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 36..192 e-value: 1.0E-23 score: 85.6
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 193..459 e-value: 2.5E-72 score: 245.8
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 39..455
None	No IPR available	PANTHER	PTHR24282:SF187	CYTOCHROME	coord: 193..455 coord: 12..192
None	No IPR available	PANTHER	PTHR24282	CYTOCHROME P450 FAMILY MEMBER	coord: 12..192
None	No IPR available	PANTHER	PTHR24282	CYTOCHROME P450 FAMILY MEMBER	coord: 193..455
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 399..408

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg026212.1	Spg026212.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen