Spg026113 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg026113
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionpolygalacturonase At1g48100-like
Locationscaffold7: 1537135 .. 1541229 (+)
RNA-Seq ExpressionSpg026113
SyntenySpg026113
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACTTCACTGGAGTTTGGTAAGTCACTATGTTAAAGCTCTATCGGCAAATTCCTGGACACGTGGCCAAATACATGGAGCTGGGGTAATAGTTTAAAGATCAAAGTGATAGCTTTGCTTAGCTTCTATGGCTTGGCGTCTCCATGCATAGACCGACTGGCGCGAGCTTTGAAGTTCCTTTGTCTATTTAAACAACCCATCTTGTTTTCCTATAAACTTGTCTTTCTCGCTTCTTCTTCCTGTTCTCTCTCTCTCAAATGTACATGAAAATGAAAGGATTTAGTTTCAGAGGGCTCACATTTTCGTTTGTGGTGGCATTTCTTGTTTGGTCTTCTAGTTTGGAGAGCTGCCATGGGAGAACCACGGGCAAGCATTGGAGGCAGAACAGAGCGGCCTCCGCCGCTCTCTCCTCCAAGAAAGGGAAGGCTCACGGTCACGGTGGTGCTCACAAAGGCAACCACCATGGTGGTGCAACAAAGACAAAGCCACCGTCTCGTAAGACCCTGCCGCCACTGCAGCCGCCGACTACAAAGCCGAAACCAGATGTCCCTCGCAGCCCGCCGCCCCAGAAGGGCGGCCATTCTTCCATGTTTAACGTGCTCGATTTTGGAGCTAAAGGTGATGGAGTAACTGATGACACTAAGGTGAACATTAATCTTATCTTCACAAAAGTATTTATGAGCTTATAATTACACGAGTATGAGCAATATACGCGAATATATGTATGTATATGGTATTTTACGGGGAAGGGAAATGGAAAAGGGAGGTGGACCCACCTAGAAAGGCGGAATCTCGTTATGGCAGTTGGGTCAGGATTTGAGCTAGAATAATTGGGTGGAGTAGGCAGCATCCTGAAAACTAACGCCAAACAGTCAAAATTTTCTTACCGTCAGAAAAACAGGGGTAACAGTAAAATAATCATCTGCATCGCAGGCATTTCAGGACACATGGGCAGCTACTTGCAAGGTGGCAGCATCAACCTGCATCGTCCCATCAAACTACGTCTTTCTAGTAGGCCCCATCTCCTTCTCTGGTCCGTACTGCCAACCCGATATCGTATTCCAGGTAACTTTCCTTCTGCTAAGCATTTCGCATCAATGCTACAAATTTCTCCAACTTCAATTTACTTGTGTCAAGTTTATCTTTATATTTTTTTAATAAACTTTATTAAGAGTTCCTATCATTAGCAAATAATATTGATTCTAGTATTTTTCAACCCTTCTACTTGTATCATCCAACCAATTGACCTGAACTTAACTAATTTCTTATTAACATTACATTCTCCATCAGAATTAATCCATTCGCTATGAGACGATGAAACTTGACTAAAGTATAGATGCTAAAATCATACTTTTCAAAGTAAATAGACAAACAATAAACTACAAAAATGAAACTTACAAATTTAATGATTTATACCTTCTTAAACCAAACCTAAATGGCAGCCATGGTGATGAGGATTTAGAATCTTTAGGTATACCCTCTAAAATCAAGTTCCAAGTCAAAGTTAAAGGGCTAGTTAGCCTTCCTAAACTAGTAGGTATGGGTTACAAACTTCAACCGCAAGGAGTGAAGCTCGCATTTGGATGTGAGAATTAAAACCTTGAATTCTTAAGGTTTGAATTCTTAACTCGATAATATAATGTTTAAATTAGTTGAACTATGGAATTGAGAATGGATTTACATTATCTTGTTTTAATTTTTTGATTTAAAAAAGTTGGACGGGACAATCATTGCACCGACAGACTTCAAAGTTTGGGGCAAAGGTCTATTACAATGGCTGCAATTCACAAAGTTGATAGGAATTACCATACAAGGAAATGGAATTATTGATGGACGGGGTTCAGTATGGTGGCATGTTTCACCTTTTGATGATCCCATTGACGATGAATTCAAACTACTTGTCCCACTCAACAGCACTGTACACGCAAAGCCACCATCCCCGGTAACTCCCCTTCTTCACACCCCACATTGTAGTTTCTAAGAATTCTCTGCAGCATTTTCTCTAAATTCAAACTGAAACCTATGGCAGGCAAGAAATGAGCTTGTGGGAAAGATGCCTGGCATCAAACCAACTGTAAGCGGCCTACTAGATCCCAAAATTCTATGAGCTCCTTTGGTTGTTTGTGAACGATCCCTTGACATGGCTCTGTTCTTTTGTTGTACAGGCTCTGAGGTTTTATGGAAGTTTTAACGTTACCGTCACTGGCATAACAATTCAGAACAGTCCTCAATGCCACCTCAAGTTCGACAATTGCATTGGAGTCTTGGTTCATGATTTTAATGTTTCATCTCCAGGTGACAGTCTCAATACTGATGGAATTCATCTGCAGAATTCTAAGGATGTGCTAATCTACAGCACCACTCTCTCTTGTGGTATGAATTACTTTGCATAGAATCATTAATTTGTTCACTGTTATTGACATTTCATCCATTCTGGGACATGGCATCATTACATAAAAGAATAAGACCAGCATAAATAAGGATCAATCAATTGTAAAAACGATTGGAAAATTCAATGACATCAACCAATGGAGTATTTTAGCAATTCGGATAGGTCCGGTGAGAACATAAAGAATTTTAGCAACCAGAGGGCTTGTCCCTCCCTCACCATCGCCATTGTCCAGCAACAAAGTGAAACACAGTACGCAAATAGCTAGTTCCCAACCTTCTCCTACTTCTTTGCTATCATACATGTAATTCGAATACAAAGACAAGGAACACATTTTTTTTTCTCCTTCACTTGTGCACTTGCACACGTTGACAAGCCTTACGAAACAACTACCCAACCCTACGACATTTGATTATCAAAAAACTCAACAAGATTTTAATATCCTATGTCAGTAGCCACGTTCTACATTTTCAATTTTTATTTGCTAACATAATTTTTTTGGCTTCAAGCTTCCCAAGAAAAAACAAATTCTTGGCAGTGACATTTATGCATCAGACATATATTGGATGAAAACTGACTACTAGGTTTGATCTTACACACAGGAGACGACTGTATTTCTATACAAACTGGTTGCTCAAACGTGTATATACACAATATCAATTGTGGGCCCGGACATGGAATCAGCATTGGAAGTCTGGGGAAGGACAACACAAAGGCTTGTGTTTCTAACATCACTGTCCGGGATGTATTTATGCATAACACAATGAATGGTGTCAGAATCAAAACGTGGCAGGTATGCATTACACCAAGCACAGTTCACCTCTCAAAATTAACTCATCAATAATGTTAGCCTCCATCGGTTAAGTAAATGCTTTATTTTGCATTAGTCTGTTATCTATGGATCAAACTAGAGGTGGATAGAAACCTATAAACAACATGCACCGTTAGGTTAAACCAGCTCTGCTCAAAACTTGATTCCAAATTATACATCTGCAGGGTGGATTAGGTTTTGTACAGGGAGTCCTCTTCTCAAATATTCAAGTTTCTGAGGTTAAGATACCGATAGTCATAGACCAATTCTACTGTGACAAAGCCAAATGCTCAAACCAAACTTCAGCTGTGGCTTTATCAGGAATAAACTATGAAAGAATAAGAGGTACTTACACAGTAAAACCAGTACACTTTGCCTGTAGTGATAATCTGCCATGTACAGATATATCACTAAGTGCAATAGAGCTCAAGCCACTTCAAGAGCTCTATCATTTATACGGGGCCTTCTGCTGGCAAACTTTTGGAGAGTTGAGAACTCCTACTGTCCCACCAATTGATTGTTTACACATTGGGAAGCCATCAAGCAACCATATTCAGTATGATTTTGATTCTTGTTAAGTGTGAAGTCGATATTTTTGGTGTATTCACTTGTGTGGTTGGCATGATTTTCCAACCCCTTTTCACACCATGAGCTTACTTTTGTACATTTTAGTAGCCCTGGTTCATTAGATTATTCTGCAAAAGTTATAGTTCTGCCCATTGAGATACAGACATGTTGCTCTGACTAGAATGCAGTAGTAGCATGCTGCTTGTTTGTCATTGCACACTTCACTGGGCTACATTATACTACAAACTCTATGAAGGTTTCTAATAAATCCAGTACTTTGTACTTTAAATTTTCGAATTTACATTGATTAGTTGAAATAAAATTCCATACC

mRNA sequence

ATGTACATGAAAATGAAAGGATTTAGTTTCAGAGGGCTCACATTTTCGTTTGTGGTGGCATTTCTTGTTTGGTCTTCTAGTTTGGAGAGCTGCCATGGGAGAACCACGGGCAAGCATTGGAGGCAGAACAGAGCGGCCTCCGCCGCTCTCTCCTCCAAGAAAGGGAAGGCTCACGGTCACGGTGGTGCTCACAAAGGCAACCACCATGGTGGTGCAACAAAGACAAAGCCACCGTCTCGTAAGACCCTGCCGCCACTGCAGCCGCCGACTACAAAGCCGAAACCAGATGTCCCTCGCAGCCCGCCGCCCCAGAAGGGCGGCCATTCTTCCATGTTTAACGTGCTCGATTTTGGAGCTAAAGGTGATGGAGTAACTGATGACACTAAGGCATTTCAGGACACATGGGCAGCTACTTGCAAGGTGGCAGCATCAACCTGCATCGTCCCATCAAACTACGTCTTTCTAGTAGGCCCCATCTCCTTCTCTGGTCCGTACTGCCAACCCGATATCGTATTCCAGGCAAGAAATGAGCTTGTGGGAAAGATGCCTGGCATCAAACCAACTGCTCTGAGGTTTTATGGAAGTTTTAACGTTACCGTCACTGGCATAACAATTCAGAACAGTCCTCAATGCCACCTCAAGTTCGACAATTGCATTGGAGTCTTGGTTCATGATTTTAATGTTTCATCTCCAGGTGACAGTCTCAATACTGATGGAATTCATCTGCAGAATTCTAAGGATGTGCTAATCTACAGCACCACTCTCTCTTGTGGAGACGACTGTATTTCTATACAAACTGGTTGCTCAAACGTGTATATACACAATATCAATTGTGGGCCCGGACATGGAATCAGCATTGGAAGTCTGGGGAAGGACAACACAAAGGCTTGTGTTTCTAACATCACTGTCCGGGATGTATTTATGCATAACACAATGAATGGTGTCAGAATCAAAACGTGGCAGGGTGGATTAGGTTTTGTACAGGGAGTCCTCTTCTCAAATATTCAAGTTTCTGAGGTTAAGATACCGATAGTCATAGACCAATTCTACTGTGACAAAGCCAAATGCTCAAACCAAACTTCAGCTGTGGCTTTATCAGGAATAAACTATGAAAGAATAAGAGGTACTTACACAGTAAAACCAGTACACTTTGCCTGTAGTGATAATCTGCCATGTACAGATATATCACTAAGTGCAATAGAGCTCAAGCCACTTCAAGAGCTCTATCATTTATACGGGGCCTTCTGCTGGCAAACTTTTGGAGAGTTGAGAACTCCTACTGTCCCACCAATTGATTGTTTACACATTGGGAAGCCATCAAGCAACCATATTCAGTATGATTTTGATTCTTGTTAA

Coding sequence (CDS)

ATGTACATGAAAATGAAAGGATTTAGTTTCAGAGGGCTCACATTTTCGTTTGTGGTGGCATTTCTTGTTTGGTCTTCTAGTTTGGAGAGCTGCCATGGGAGAACCACGGGCAAGCATTGGAGGCAGAACAGAGCGGCCTCCGCCGCTCTCTCCTCCAAGAAAGGGAAGGCTCACGGTCACGGTGGTGCTCACAAAGGCAACCACCATGGTGGTGCAACAAAGACAAAGCCACCGTCTCGTAAGACCCTGCCGCCACTGCAGCCGCCGACTACAAAGCCGAAACCAGATGTCCCTCGCAGCCCGCCGCCCCAGAAGGGCGGCCATTCTTCCATGTTTAACGTGCTCGATTTTGGAGCTAAAGGTGATGGAGTAACTGATGACACTAAGGCATTTCAGGACACATGGGCAGCTACTTGCAAGGTGGCAGCATCAACCTGCATCGTCCCATCAAACTACGTCTTTCTAGTAGGCCCCATCTCCTTCTCTGGTCCGTACTGCCAACCCGATATCGTATTCCAGGCAAGAAATGAGCTTGTGGGAAAGATGCCTGGCATCAAACCAACTGCTCTGAGGTTTTATGGAAGTTTTAACGTTACCGTCACTGGCATAACAATTCAGAACAGTCCTCAATGCCACCTCAAGTTCGACAATTGCATTGGAGTCTTGGTTCATGATTTTAATGTTTCATCTCCAGGTGACAGTCTCAATACTGATGGAATTCATCTGCAGAATTCTAAGGATGTGCTAATCTACAGCACCACTCTCTCTTGTGGAGACGACTGTATTTCTATACAAACTGGTTGCTCAAACGTGTATATACACAATATCAATTGTGGGCCCGGACATGGAATCAGCATTGGAAGTCTGGGGAAGGACAACACAAAGGCTTGTGTTTCTAACATCACTGTCCGGGATGTATTTATGCATAACACAATGAATGGTGTCAGAATCAAAACGTGGCAGGGTGGATTAGGTTTTGTACAGGGAGTCCTCTTCTCAAATATTCAAGTTTCTGAGGTTAAGATACCGATAGTCATAGACCAATTCTACTGTGACAAAGCCAAATGCTCAAACCAAACTTCAGCTGTGGCTTTATCAGGAATAAACTATGAAAGAATAAGAGGTACTTACACAGTAAAACCAGTACACTTTGCCTGTAGTGATAATCTGCCATGTACAGATATATCACTAAGTGCAATAGAGCTCAAGCCACTTCAAGAGCTCTATCATTTATACGGGGCCTTCTGCTGGCAAACTTTTGGAGAGTTGAGAACTCCTACTGTCCCACCAATTGATTGTTTACACATTGGGAAGCCATCAAGCAACCATATTCAGTATGATTTTGATTCTTGTTAA

Protein sequence

MYMKMKGFSFRGLTFSFVVAFLVWSSSLESCHGRTTGKHWRQNRAASAALSSKKGKAHGHGGAHKGNHHGGATKTKPPSRKTLPPLQPPTTKPKPDVPRSPPPQKGGHSSMFNVLDFGAKGDGVTDDTKAFQDTWAATCKVAASTCIVPSNYVFLVGPISFSGPYCQPDIVFQARNELVGKMPGIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVHDFNVSSPGDSLNTDGIHLQNSKDVLIYSTTLSCGDDCISIQTGCSNVYIHNINCGPGHGISIGSLGKDNTKACVSNITVRDVFMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLSAIELKPLQELYHLYGAFCWQTFGELRTPTVPPIDCLHIGKPSSNHIQYDFDSC
Homology
BLAST of Spg026113 vs. NCBI nr
Match: XP_008446197.1 (PREDICTED: polygalacturonase At1g48100-like [Cucumis melo] >XP_016900181.1 PREDICTED: polygalacturonase At1g48100-like [Cucumis melo] >ADN33828.1 polygalacturonase [Cucumis melo subsp. melo] >TYK15613.1 polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 817.8 bits (2111), Expect = 4.7e-233
Identity = 413/530 (77.92%), Postives = 424/530 (80.00%), Query Frame = 0

Query: 1   MYMKMKGFSFRGLTFSFVVAFLVWSSSLESCHGRTTGKH-WRQNRAASAALSSKKGKAHG 60
           MYMKM+G SFRGLTF F VAFLVWSSSLE CHGR   KH WRQNR AS AL SKKGKAH 
Sbjct: 1   MYMKMEGISFRGLTFMFAVAFLVWSSSLEGCHGRVRSKHYWRQNRVASPALYSKKGKAHS 60

Query: 61  HGGAHKGNHHGGATKTKP-PSRKTLPPLQPPTTKPKPDVPRSPPPQKGGHSSMFNVLDFG 120
           HGG+HKGNHHG A+KTKP PS KT  PL  P TKPKP++PRSPPPQKGGHS MFNVLDFG
Sbjct: 61  HGGSHKGNHHGSASKTKPRPSHKTPQPLPRPVTKPKPNIPRSPPPQKGGHSFMFNVLDFG 120

Query: 121 AKGDGVTDDTKAFQDTWAATCKVAASTCIVPSNYVFLVGPISFSGPYCQPDIVFQ----- 180
           AKGDG TDDTKAFQDTW A+CKVAAST IVPS YVFLVGPISFSGPYCQPDIVFQ     
Sbjct: 121 AKGDGKTDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGTI 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 IAPTDFKVWGKGLLQWLQFTKLMGLTIQGNGVIDGRGSVWWQEISPYDDPIDDEFKLLIP 240

Query: 241 ------------ARNELVGKMPGIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGV 300
                       AR+ELVGKMP IKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGV
Sbjct: 241 LNSTVQEKPPAPARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGV 300

Query: 301 LVHDFNVSSPGDSLNTDGIHLQNSKDVLIYSTTLSCGDDCISIQTGCSNVYIHNINCGPG 360
           LVHDFNVSSPGDSLNTDGIHLQNSKDVLIYS+TLSCGDDCISIQTGCSNVYIHN+NCGPG
Sbjct: 301 LVHDFNVSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPG 360

Query: 361 HGISIGSLGKDNTKACVSNITVRDVFMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVK 420
           HGISIGSLGKDNTKACVSNITVRDV MHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVK
Sbjct: 361 HGISIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVK 420

Query: 421 IPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLSAIE 452
           IPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISL+AIE
Sbjct: 421 IPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLTAIE 480

BLAST of Spg026113 vs. NCBI nr
Match: XP_038893387.1 (polygalacturonase At1g48100-like isoform X2 [Benincasa hispida])

HSP 1 Score: 816.6 bits (2108), Expect = 1.1e-232
Identity = 412/529 (77.88%), Postives = 425/529 (80.34%), Query Frame = 0

Query: 1   MYMKMKGFSFRGLTFSFVVAFLVWSSSLESCHGRTTGKH-WRQNRAASAALSSKKGKAHG 60
           MYMKMKG SFRGLTF  +VAFLVWSSSLE CHGR T KH WR+NR AS ALSSKKGKAHG
Sbjct: 1   MYMKMKGISFRGLTFMCLVAFLVWSSSLEGCHGRATSKHYWRKNRVASPALSSKKGKAHG 60

Query: 61  HGGAHKGNHHGGATKTK-PPSRKTLPPLQPPTTKPKPDVPRSPPPQKGGHSSMFNVLDFG 120
           HGG+HKGNHHG A+KTK PPSRKT  PL PP  KPKPD+PRSPPPQKGGHSS+FNVLDFG
Sbjct: 61  HGGSHKGNHHGSASKTKPPPSRKTPQPLPPPVAKPKPDIPRSPPPQKGGHSSIFNVLDFG 120

Query: 121 AKGDGVTDDTKAFQDTWAATCKVAASTCIVPSNYVFLVGPISFSGPYCQPDIVFQ----- 180
           AKGDG TDDTKAFQDTW A+CKVAAST IVPS YVFLVGPISFSGPYCQP+IVFQ     
Sbjct: 121 AKGDGETDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPNIVFQLDGTI 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 IAPTDFKVWGKGLLQWLQFTKLKGLTIQGNGVIDGRGSAWWQVSPYDDPIEDEFKQLIPL 240

Query: 241 -----------ARNELVGKMPGIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVL 300
                      AR+EL GKMP IKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVL
Sbjct: 241 NSTLKEKPPAPARSELEGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVL 300

Query: 301 VHDFNVSSPGDSLNTDGIHLQNSKDVLIYSTTLSCGDDCISIQTGCSNVYIHNINCGPGH 360
           VHDF VSSPGDSLNTDGIHLQNSKDVLIYS+TLSCGDDCISIQTGCSNVYIHN+NCGPGH
Sbjct: 301 VHDFFVSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPGH 360

Query: 361 GISIGSLGKDNTKACVSNITVRDVFMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKI 420
           GISIGSLGKDNTKACVSNITVRD+ MHNTM GVRIKTWQGGLGFVQGVLFSNIQVSEVKI
Sbjct: 361 GISIGSLGKDNTKACVSNITVRDITMHNTMTGVRIKTWQGGLGFVQGVLFSNIQVSEVKI 420

Query: 421 PIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLSAIEL 452
           PIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPC DISL+AIEL
Sbjct: 421 PIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCADISLTAIEL 480

BLAST of Spg026113 vs. NCBI nr
Match: XP_038893386.1 (polygalacturonase At1g48100-like isoform X1 [Benincasa hispida])

HSP 1 Score: 810.4 bits (2092), Expect = 7.5e-231
Identity = 412/534 (77.15%), Postives = 425/534 (79.59%), Query Frame = 0

Query: 1   MYMKMKGFSFRGLTFSFVVAFLVWSSSLESCHGRTTGKH-WRQNRAASAALSSKKGKAHG 60
           MYMKMKG SFRGLTF  +VAFLVWSSSLE CHGR T KH WR+NR AS ALSSKKGKAHG
Sbjct: 1   MYMKMKGISFRGLTFMCLVAFLVWSSSLEGCHGRATSKHYWRKNRVASPALSSKKGKAHG 60

Query: 61  HGGAHKGNHHGGATKTK-PPSRKTLPPLQPPTTKPKPDVPRSPPPQKGGHSSMFNVLDFG 120
           HGG+HKGNHHG A+KTK PPSRKT  PL PP  KPKPD+PRSPPPQKGGHSS+FNVLDFG
Sbjct: 61  HGGSHKGNHHGSASKTKPPPSRKTPQPLPPPVAKPKPDIPRSPPPQKGGHSSIFNVLDFG 120

Query: 121 AKGDGVTDDTKAFQDTWAATCKVAASTCIVPSNYVFLVGPISFSGPYCQPDIVFQ----- 180
           AKGDG TDDTKAFQDTW A+CKVAAST IVPS YVFLVGPISFSGPYCQP+IVFQ     
Sbjct: 121 AKGDGETDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPNIVFQLDGTI 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 IAPTDFKVWGKGLLQWLQFTKLKGLTIQGNGVIDGRGSAWWQVSPYDDPIEDEFKQLIPL 240

Query: 241 -----------ARNELVGKMPGIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVL 300
                      AR+EL GKMP IKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVL
Sbjct: 241 NSTLKEKPPAPARSELEGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVL 300

Query: 301 VHDFNVSSPGDSLNTDGIHLQNSKDVLIYSTTLSCGDDCISIQTGCSNVYIHNINCGPGH 360
           VHDF VSSPGDSLNTDGIHLQNSKDVLIYS+TLSCGDDCISIQTGCSNVYIHN+NCGPGH
Sbjct: 301 VHDFFVSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPGH 360

Query: 361 GISIGSLGKDNTKACVSNITVRDVFMHNTMNGVRIKTW-----QGGLGFVQGVLFSNIQV 420
           GISIGSLGKDNTKACVSNITVRD+ MHNTM GVRIKTW     QGGLGFVQGVLFSNIQV
Sbjct: 361 GISIGSLGKDNTKACVSNITVRDITMHNTMTGVRIKTWQTVHLQGGLGFVQGVLFSNIQV 420

Query: 421 SEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISL 452
           SEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPC DISL
Sbjct: 421 SEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCADISL 480

BLAST of Spg026113 vs. NCBI nr
Match: XP_004135250.1 (polygalacturonase At1g48100 [Cucumis sativus] >KGN51797.1 hypothetical protein Csa_008759 [Cucumis sativus])

HSP 1 Score: 805.4 bits (2079), Expect = 2.4e-229
Identity = 407/530 (76.79%), Postives = 421/530 (79.43%), Query Frame = 0

Query: 1   MYMKMKGFSFRGLTFSFVVAFLVWSSSLESCHGRTTGKH-WRQNRAASAALSSKKGKAHG 60
           MYMKM+G SFRGLTF   VAFLVWSSSLE CHG+   KH WR+NR AS AL SKKGKAH 
Sbjct: 1   MYMKMEGISFRGLTFMLAVAFLVWSSSLEGCHGKVRSKHYWRKNRVASPALYSKKGKAHS 60

Query: 61  HGGAHKGNHHGGATKTKP-PSRKTLPPLQPPTTKPKPDVPRSPPPQKGGHSSMFNVLDFG 120
           HGG+HKGNHHG A+KTKP PS KT  PL  P TKPKP++PRSPPPQK GHS MFNVLDFG
Sbjct: 61  HGGSHKGNHHGSASKTKPRPSHKTPQPLPRPVTKPKPNIPRSPPPQKEGHSIMFNVLDFG 120

Query: 121 AKGDGVTDDTKAFQDTWAATCKVAASTCIVPSNYVFLVGPISFSGPYCQPDIVFQ----- 180
           AKGDG TDDTKAFQDTW A+CKVAAST IVPS YVFLVGPISFSGPYCQPDIVFQ     
Sbjct: 121 AKGDGKTDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGTI 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 IAPTDFKVWGKGLLQWLQFTKLMGLTIQGNGVIDGRGSVWWQEISPYDDPIDDEFKLLIP 240

Query: 241 ------------ARNELVGKMPGIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGV 300
                       AR+ELVGKMP IKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGV
Sbjct: 241 LNSTVQEKPPPPARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGV 300

Query: 301 LVHDFNVSSPGDSLNTDGIHLQNSKDVLIYSTTLSCGDDCISIQTGCSNVYIHNINCGPG 360
           LVHDFNVSSPGDSLNTDGIHLQNSKDVLIYS+TLSCGDDCISIQTGCSNVYIHN+NCGPG
Sbjct: 301 LVHDFNVSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPG 360

Query: 361 HGISIGSLGKDNTKACVSNITVRDVFMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVK 420
           HGISIGSLGKDNTKACVSNITVRDV MHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVK
Sbjct: 361 HGISIGSLGKDNTKACVSNITVRDVTMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVK 420

Query: 421 IPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLSAIE 452
           IPIVIDQFYCDKAKCSNQT+AVALSGINYERIRGTYTVKPVHFACSDNLPCTDISL+AIE
Sbjct: 421 IPIVIDQFYCDKAKCSNQTAAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLTAIE 480

BLAST of Spg026113 vs. NCBI nr
Match: KAA0034307.1 (polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 801.6 bits (2069), Expect = 3.5e-228
Identity = 413/561 (73.62%), Postives = 424/561 (75.58%), Query Frame = 0

Query: 1   MYMKMKGFSFRGLTFSFVVAFLVWSSSLESCHGRTTGKH-WRQNRAASAALSSKKGKAHG 60
           MYMKM+G SFRGLTF F VAFLVWSSSLE CHGR   KH WRQNR AS AL SKKGKAH 
Sbjct: 1   MYMKMEGISFRGLTFMFAVAFLVWSSSLEGCHGRVRSKHYWRQNRVASPALYSKKGKAHS 60

Query: 61  HGGAHKGNHHGGATKTKP-PSRKTLPPLQPPTTKPKPDVPRSPPPQKGGHSSMFNVLDFG 120
           HGG+HKGNHHG A+KTKP PS KT  PL  P TKPKP++PRSPPPQKGGHS MFNVLDFG
Sbjct: 61  HGGSHKGNHHGSASKTKPRPSHKTPQPLPRPVTKPKPNIPRSPPPQKGGHSFMFNVLDFG 120

Query: 121 AKGDGVTDDTK-------------------------------AFQDTWAATCKVAASTCI 180
           AKGDG TDDTK                               AFQDTW A+CKVAAST I
Sbjct: 121 AKGDGKTDDTKYVWWKWYFTGKGNGKGGVGPSRKAESRYGSWAFQDTWTASCKVAASTFI 180

Query: 181 VPSNYVFLVGPISFSGPYCQPDIVFQ---------------------------------- 240
           VPS YVFLVGPISFSGPYCQPDIVFQ                                  
Sbjct: 181 VPSKYVFLVGPISFSGPYCQPDIVFQLDGTIIAPTDFKVWGKGLLQWLQFTKLMGLTIQG 240

Query: 241 -------------------------------------------ARNELVGKMPGIKPTAL 300
                                                      AR+ELVGKMP IKPTAL
Sbjct: 241 NGVIDGRGSVWWQEISPYDDPIDDEFKLLIPLNSTVQEKPPAPARSELVGKMPRIKPTAL 300

Query: 301 RFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVHDFNVSSPGDSLNTDGIHLQNSKDVLI 360
           RFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVHDFNVSSPGDSLNTDGIHLQNSKDVLI
Sbjct: 301 RFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVHDFNVSSPGDSLNTDGIHLQNSKDVLI 360

Query: 361 YSTTLSCGDDCISIQTGCSNVYIHNINCGPGHGISIGSLGKDNTKACVSNITVRDVFMHN 420
           YS+TLSCGDDCISIQTGCSNVYIHN+NCGPGHGISIGSLGKDNTKACVSNITVRDV MHN
Sbjct: 361 YSSTLSCGDDCISIQTGCSNVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHN 420

Query: 421 TMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINY 452
           TMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINY
Sbjct: 421 TMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINY 480

BLAST of Spg026113 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 6.0e-90
Identity = 189/439 (43.05%), Postives = 243/439 (55.35%), Query Frame = 0

Query: 64  HKGNHHGGATKTKPPSRKTLPPLQ---PPTTKPKPDVPRSPPPQKGGHSS--MFNVLDFG 123
           HKG H  G T   PP     PPL+   PP   P    P SP P  G   S  +F+V  FG
Sbjct: 32  HKGRH--GVTHPLPPPPP--PPLETANPPDQVPSDPYP-SPDPAPGDSDSGCVFDVTSFG 91

Query: 124 AKGDGVTDDTKAFQDTWAATCKVAASTCIVPSNYVFLVGPISFSGPYCQPDIVFQARNEL 183
           A GDG  DDT AFQD W A C V +   + P   VF +    FSGP C+P +VFQ    L
Sbjct: 92  AVGDGSCDDTAAFQDAWKAACAVESGVVLAPEGGVFKITSTIFSGP-CKPGLVFQLDGVL 151

Query: 184 V--------------------------------------------------------GKM 243
           +                                                           
Sbjct: 152 MPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQKWWDLPCKPHRGPDGSSSSG 211

Query: 244 PGIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVHDFNVSSPGDSLNTDGIHL 303
           P   PT +RF+ S N+ V G+ IQNSPQ H+KFD C GVL+++  +SSP  S NTDGIHL
Sbjct: 212 PCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGVLINEIQISSPKLSPNTDGIHL 271

Query: 304 QNSKDVLIYSTTLSCGDDCISIQTGCSNVYIHNINCGPGHGISIGSLGKDNTKACVSNIT 363
            N++ V IY++ +S GDDCISI TGCS+V I  + CGP HGISIGSLG  N++ACVSNIT
Sbjct: 272 GNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPSHGISIGSLGVHNSQACVSNIT 331

Query: 364 VRDVFMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSA 423
           VR+  + ++ NG+R+KTWQGG G V  +LF NIQ+  V   I++DQ+YC    C N+TSA
Sbjct: 332 VRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMENVLNCIIVDQYYCQSKDCRNETSA 391

Query: 424 VALSGINYERIRGTYTVK--PVHFACSDNLPCTDISLSAIELKPLQELYHLYGAFCWQTF 440
           V +  + Y  I+GTY V+  P+HFACSD + CT+I++S +EL P +E   +   FCW  +
Sbjct: 392 VKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITMSEVELLP-EEGELVDDPFCWNAY 451

BLAST of Spg026113 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 8.8e-65
Identity = 147/397 (37.03%), Postives = 205/397 (51.64%), Query Frame = 0

Query: 86  LQPPTTKPKPDVPRSPPPQKGGHSSMFNVLDFGAKGDGVTDDTKAFQDTWAATCKVAAST 145
           LQ P  + +   PRS          + +V +FGAKG+GVTDDTKAF D W   C     T
Sbjct: 35  LQLPRRQSRSTRPRS--------ERLLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKT 94

Query: 146 -CIVPSNYVFLVGPISFSGPYCQPDIVFQARNELV------------------------- 205
             +VP NY  L+ PI  SGP C+  +  Q    ++                         
Sbjct: 95  RILVPENYTCLLRPIDLSGP-CKARLTLQISGTIIAPNDPDVWEGLNRRKWLYFHGLSRL 154

Query: 206 -----GKMPGI-------------------KPTALRFYGSFNVTVTGITIQNSPQCHLKF 265
                G + G+                    PTAL F+   N+ V  + + +S Q H+  
Sbjct: 155 TVEGGGTVNGMGQEWWERSCKHNHSNPCRGAPTALTFHKCKNMRVENLNVIDSQQMHIAL 214

Query: 266 DNCIGVLVHDFNVSSPGDSLNTDGIHLQNSKDVLIYSTTLSCGDDCISIQTGCSNVYIHN 325
            +C  V +    V +P  S NTDGIH+  S+ ++I +TT+S GDDCISI    + + I N
Sbjct: 215 TSCRRVTISGLKVIAPATSPNTDGIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISN 274

Query: 326 INCGPGHGISIGSLGKDNTKACVSNITVRDVFMHNTMNGVRIKTWQGGLGFVQGVLFSNI 385
           I CGPGHGISIGSLGK  +   V +ITV    + +T NGVRIKTWQGG G V  ++F NI
Sbjct: 275 IICGPGHGISIGSLGKSKSWEEVRDITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNI 334

Query: 386 QVSEVKIPIVIDQFYCDKAK-CSNQTSAVALSGINYERIRGTYTVK-PVHFACSDNLPCT 431
           +++ V  PI+IDQ+YCD  K C+NQTSA+++  I++  +RGT   K  +  +CSD+ PC 
Sbjct: 335 KMNNVSNPIIIDQYYCDSRKPCANQTSAISIENISFVHVRGTSASKEAIKISCSDSSPCR 394

BLAST of Spg026113 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 1.1e-62
Identity = 135/347 (38.90%), Postives = 187/347 (53.89%), Query Frame = 0

Query: 109 SSMFNVLDFGAKGDGVTDDTKAFQDTWAATCKVAASTCIVPSNYVFLVGPISFSGPYCQP 168
           +++FNV  +GA GDG  D T+AF  TW A CK A++  +VP+N  F V  + F GP CQP
Sbjct: 56  ATVFNVEQYGAVGDGKHDSTEAFATTWNAACKKASAVLLVPANKKFFVNNLVFRGP-CQP 115

Query: 169 DIVFQARNELV----------------------------GKMPG---------------- 228
            + F+    +V                            G + G                
Sbjct: 116 HLSFKVDGTIVAQPDPARWKNSKIWLQFAQLTDFNLMGTGVIDGQGQQWWAGQCKVVNGR 175

Query: 229 ------IKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVHDFNVSSPGDSLNTD 288
                  +PTA++   S +VTV  +T+ NSP+ HL F  C GV +    + +P DS NTD
Sbjct: 176 TVCNDRNRPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVKIQGLKIKAPRDSPNTD 235

Query: 289 GIHLQNSKDVLIYSTTLSCGDDCISIQTGCSNVYIHNINCGPGHGISIGSLGKDNTKACV 348
           GI +  SK   I    +  GDDCI+I TG SN+ I ++ CGPGHGISIGSLG+DN++A V
Sbjct: 236 GIDIFASKRFHIEKCVIGTGDDCIAIGTGSSNITIKDLICGPGHGISIGSLGRDNSRAEV 295

Query: 349 SNITVRDVFMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIVIDQFYCDKAK-CS 404
           S++ V      +T NG+RIKTWQGG G    + + N+++   + PI+I+QFYC  A  C 
Sbjct: 296 SHVHVNRAKFIDTQNGLRIKTWQGGSGLASYITYENVEMINSENPILINQFYCTSASACQ 355

BLAST of Spg026113 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 1.4e-62
Identity = 131/346 (37.86%), Postives = 189/346 (54.62%), Query Frame = 0

Query: 110 SMFNVLDFGAKGDGVTDDTKAFQDTWAATCKVAASTCIVPSNYVFLVGPISFSGPYCQPD 169
           ++FNV  +GA GDG  D T+AF   W A CK  ++  +VP N  F+V  + F+GP CQP 
Sbjct: 57  NIFNVEKYGAVGDGKHDCTEAFSTAWQAACKKPSAMLLVPGNKKFVVNNLFFNGP-CQPH 116

Query: 170 IVFQA-------------RNELV----GKMPGI--------------------------- 229
             F+              +N  +     K+ G                            
Sbjct: 117 FTFKVDGIIAAYQNPASWKNNRIWLQFAKLTGFTLMGKGVIDGQGKQWWAGQCKWVNGRE 176

Query: 230 ------KPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVHDFNVSSPGDSLNTDG 289
                 +PTA++F  S  + + G+ + NSP+ HL F NC GV +   ++++P DS NTDG
Sbjct: 177 ICNDRDRPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEGVKIIGISITAPRDSPNTDG 236

Query: 290 IHLQNSKDVLIYSTTLSCGDDCISIQTGCSNVYIHNINCGPGHGISIGSLGKDNTKACVS 349
           I +  SK+  +   T+  GDDC++I TG SN+ I ++ CGPGHGISIGSLG++N++A VS
Sbjct: 237 IDIFASKNFHLQKNTIGTGDDCVAIGTGSSNIVIEDLICGPGHGISIGSLGRENSRAEVS 296

Query: 350 NITVRDVFMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIVIDQFYCDKAK-CSN 404
            + V      +T NG+RIKTWQGG G    +++ N+++   + PI+I+QFYC  A  C N
Sbjct: 297 YVHVNGAKFIDTQNGLRIKTWQGGSGMASHIIYENVEMINSENPILINQFYCTSASACQN 356

BLAST of Spg026113 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 234.2 bits (596), Expect = 2.9e-60
Identity = 132/341 (38.71%), Postives = 181/341 (53.08%), Query Frame = 0

Query: 113 NVLDFGAKGDGVTDDTKAFQDTWAATCKV-AASTCIVPSNYVFLVGPISFSGPY------ 172
           +V DFGAKGDG TDDT+AF + W   C    A   +VP    +L+  I  +GP       
Sbjct: 69  SVSDFGAKGDGKTDDTQAFVNAWKKACSSNGAVNLLVPKGNTYLLKSIQLTGPCNSILTV 128

Query: 173 -----------------CQPDIVFQARNEL------VGKMPG------------------ 232
                                I+F   N L       G + G                  
Sbjct: 129 QIFGTLSASQKRSDYKDISKWIMFDGVNNLSVDGGDTGVVDGNGETWWQNSCKRNKAKPC 188

Query: 233 -IKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVHDFNVSSPGDSLNTDGIHLQ 292
              PTAL FY S ++ V  + ++N+ Q  +  + C  V V +  V++P DS NTDGIH+ 
Sbjct: 189 TKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQVSNVVVTAPADSPNTDGIHIT 248

Query: 293 NSKDVLIYSTTLSCGDDCISIQTGCSNVYIHNINCGPGHGISIGSLGKDNTKACVSNITV 352
           N++++ +  + +  GDDCISI++G  NV I++I CGPGHGISIGSLG DN+KA VS +TV
Sbjct: 249 NTQNIRVSESIIGTGDDCISIESGSQNVQINDITCGPGHGISIGSLGDDNSKAFVSGVTV 308

Query: 353 RDVFMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAV 404
               +  T NGVRIKT+QGG G    ++F NIQ+  VK PI+IDQ YCDK+KC+ + SAV
Sbjct: 309 DGAKLSGTDNGVRIKTYQGGSGTASNIIFQNIQMDNVKNPIIIDQDYCDKSKCTTEKSAV 368

BLAST of Spg026113 vs. ExPASy TrEMBL
Match: A0A1S3BFB4 (polygalacturonase At1g48100-like OS=Cucumis melo OX=3656 GN=LOC103489001 PE=3 SV=1)

HSP 1 Score: 817.8 bits (2111), Expect = 2.3e-233
Identity = 413/530 (77.92%), Postives = 424/530 (80.00%), Query Frame = 0

Query: 1   MYMKMKGFSFRGLTFSFVVAFLVWSSSLESCHGRTTGKH-WRQNRAASAALSSKKGKAHG 60
           MYMKM+G SFRGLTF F VAFLVWSSSLE CHGR   KH WRQNR AS AL SKKGKAH 
Sbjct: 1   MYMKMEGISFRGLTFMFAVAFLVWSSSLEGCHGRVRSKHYWRQNRVASPALYSKKGKAHS 60

Query: 61  HGGAHKGNHHGGATKTKP-PSRKTLPPLQPPTTKPKPDVPRSPPPQKGGHSSMFNVLDFG 120
           HGG+HKGNHHG A+KTKP PS KT  PL  P TKPKP++PRSPPPQKGGHS MFNVLDFG
Sbjct: 61  HGGSHKGNHHGSASKTKPRPSHKTPQPLPRPVTKPKPNIPRSPPPQKGGHSFMFNVLDFG 120

Query: 121 AKGDGVTDDTKAFQDTWAATCKVAASTCIVPSNYVFLVGPISFSGPYCQPDIVFQ----- 180
           AKGDG TDDTKAFQDTW A+CKVAAST IVPS YVFLVGPISFSGPYCQPDIVFQ     
Sbjct: 121 AKGDGKTDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGTI 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 IAPTDFKVWGKGLLQWLQFTKLMGLTIQGNGVIDGRGSVWWQEISPYDDPIDDEFKLLIP 240

Query: 241 ------------ARNELVGKMPGIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGV 300
                       AR+ELVGKMP IKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGV
Sbjct: 241 LNSTVQEKPPAPARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGV 300

Query: 301 LVHDFNVSSPGDSLNTDGIHLQNSKDVLIYSTTLSCGDDCISIQTGCSNVYIHNINCGPG 360
           LVHDFNVSSPGDSLNTDGIHLQNSKDVLIYS+TLSCGDDCISIQTGCSNVYIHN+NCGPG
Sbjct: 301 LVHDFNVSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPG 360

Query: 361 HGISIGSLGKDNTKACVSNITVRDVFMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVK 420
           HGISIGSLGKDNTKACVSNITVRDV MHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVK
Sbjct: 361 HGISIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVK 420

Query: 421 IPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLSAIE 452
           IPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISL+AIE
Sbjct: 421 IPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLTAIE 480

BLAST of Spg026113 vs. ExPASy TrEMBL
Match: A0A5D3CUP5 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001100 PE=3 SV=1)

HSP 1 Score: 817.8 bits (2111), Expect = 2.3e-233
Identity = 413/530 (77.92%), Postives = 424/530 (80.00%), Query Frame = 0

Query: 1   MYMKMKGFSFRGLTFSFVVAFLVWSSSLESCHGRTTGKH-WRQNRAASAALSSKKGKAHG 60
           MYMKM+G SFRGLTF F VAFLVWSSSLE CHGR   KH WRQNR AS AL SKKGKAH 
Sbjct: 1   MYMKMEGISFRGLTFMFAVAFLVWSSSLEGCHGRVRSKHYWRQNRVASPALYSKKGKAHS 60

Query: 61  HGGAHKGNHHGGATKTKP-PSRKTLPPLQPPTTKPKPDVPRSPPPQKGGHSSMFNVLDFG 120
           HGG+HKGNHHG A+KTKP PS KT  PL  P TKPKP++PRSPPPQKGGHS MFNVLDFG
Sbjct: 61  HGGSHKGNHHGSASKTKPRPSHKTPQPLPRPVTKPKPNIPRSPPPQKGGHSFMFNVLDFG 120

Query: 121 AKGDGVTDDTKAFQDTWAATCKVAASTCIVPSNYVFLVGPISFSGPYCQPDIVFQ----- 180
           AKGDG TDDTKAFQDTW A+CKVAAST IVPS YVFLVGPISFSGPYCQPDIVFQ     
Sbjct: 121 AKGDGKTDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGTI 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 IAPTDFKVWGKGLLQWLQFTKLMGLTIQGNGVIDGRGSVWWQEISPYDDPIDDEFKLLIP 240

Query: 241 ------------ARNELVGKMPGIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGV 300
                       AR+ELVGKMP IKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGV
Sbjct: 241 LNSTVQEKPPAPARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGV 300

Query: 301 LVHDFNVSSPGDSLNTDGIHLQNSKDVLIYSTTLSCGDDCISIQTGCSNVYIHNINCGPG 360
           LVHDFNVSSPGDSLNTDGIHLQNSKDVLIYS+TLSCGDDCISIQTGCSNVYIHN+NCGPG
Sbjct: 301 LVHDFNVSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPG 360

Query: 361 HGISIGSLGKDNTKACVSNITVRDVFMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVK 420
           HGISIGSLGKDNTKACVSNITVRDV MHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVK
Sbjct: 361 HGISIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVK 420

Query: 421 IPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLSAIE 452
           IPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISL+AIE
Sbjct: 421 IPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLTAIE 480

BLAST of Spg026113 vs. ExPASy TrEMBL
Match: E5GBI6 (Polygalacturonase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 817.8 bits (2111), Expect = 2.3e-233
Identity = 413/530 (77.92%), Postives = 424/530 (80.00%), Query Frame = 0

Query: 1   MYMKMKGFSFRGLTFSFVVAFLVWSSSLESCHGRTTGKH-WRQNRAASAALSSKKGKAHG 60
           MYMKM+G SFRGLTF F VAFLVWSSSLE CHGR   KH WRQNR AS AL SKKGKAH 
Sbjct: 1   MYMKMEGISFRGLTFMFAVAFLVWSSSLEGCHGRVRSKHYWRQNRVASPALYSKKGKAHS 60

Query: 61  HGGAHKGNHHGGATKTKP-PSRKTLPPLQPPTTKPKPDVPRSPPPQKGGHSSMFNVLDFG 120
           HGG+HKGNHHG A+KTKP PS KT  PL  P TKPKP++PRSPPPQKGGHS MFNVLDFG
Sbjct: 61  HGGSHKGNHHGSASKTKPRPSHKTPQPLPRPVTKPKPNIPRSPPPQKGGHSFMFNVLDFG 120

Query: 121 AKGDGVTDDTKAFQDTWAATCKVAASTCIVPSNYVFLVGPISFSGPYCQPDIVFQ----- 180
           AKGDG TDDTKAFQDTW A+CKVAAST IVPS YVFLVGPISFSGPYCQPDIVFQ     
Sbjct: 121 AKGDGKTDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGTI 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 IAPTDFKVWGKGLLQWLQFTKLMGLTIQGNGVIDGRGSVWWQEISPYDDPIDDEFKLLIP 240

Query: 241 ------------ARNELVGKMPGIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGV 300
                       AR+ELVGKMP IKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGV
Sbjct: 241 LNSTVQEKPPAPARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGV 300

Query: 301 LVHDFNVSSPGDSLNTDGIHLQNSKDVLIYSTTLSCGDDCISIQTGCSNVYIHNINCGPG 360
           LVHDFNVSSPGDSLNTDGIHLQNSKDVLIYS+TLSCGDDCISIQTGCSNVYIHN+NCGPG
Sbjct: 301 LVHDFNVSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPG 360

Query: 361 HGISIGSLGKDNTKACVSNITVRDVFMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVK 420
           HGISIGSLGKDNTKACVSNITVRDV MHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVK
Sbjct: 361 HGISIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVK 420

Query: 421 IPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLSAIE 452
           IPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISL+AIE
Sbjct: 421 IPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLTAIE 480

BLAST of Spg026113 vs. ExPASy TrEMBL
Match: A0A0A0KTN9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G601470 PE=3 SV=1)

HSP 1 Score: 805.4 bits (2079), Expect = 1.2e-229
Identity = 407/530 (76.79%), Postives = 421/530 (79.43%), Query Frame = 0

Query: 1   MYMKMKGFSFRGLTFSFVVAFLVWSSSLESCHGRTTGKH-WRQNRAASAALSSKKGKAHG 60
           MYMKM+G SFRGLTF   VAFLVWSSSLE CHG+   KH WR+NR AS AL SKKGKAH 
Sbjct: 1   MYMKMEGISFRGLTFMLAVAFLVWSSSLEGCHGKVRSKHYWRKNRVASPALYSKKGKAHS 60

Query: 61  HGGAHKGNHHGGATKTKP-PSRKTLPPLQPPTTKPKPDVPRSPPPQKGGHSSMFNVLDFG 120
           HGG+HKGNHHG A+KTKP PS KT  PL  P TKPKP++PRSPPPQK GHS MFNVLDFG
Sbjct: 61  HGGSHKGNHHGSASKTKPRPSHKTPQPLPRPVTKPKPNIPRSPPPQKEGHSIMFNVLDFG 120

Query: 121 AKGDGVTDDTKAFQDTWAATCKVAASTCIVPSNYVFLVGPISFSGPYCQPDIVFQ----- 180
           AKGDG TDDTKAFQDTW A+CKVAAST IVPS YVFLVGPISFSGPYCQPDIVFQ     
Sbjct: 121 AKGDGKTDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGTI 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 IAPTDFKVWGKGLLQWLQFTKLMGLTIQGNGVIDGRGSVWWQEISPYDDPIDDEFKLLIP 240

Query: 241 ------------ARNELVGKMPGIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGV 300
                       AR+ELVGKMP IKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGV
Sbjct: 241 LNSTVQEKPPPPARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGV 300

Query: 301 LVHDFNVSSPGDSLNTDGIHLQNSKDVLIYSTTLSCGDDCISIQTGCSNVYIHNINCGPG 360
           LVHDFNVSSPGDSLNTDGIHLQNSKDVLIYS+TLSCGDDCISIQTGCSNVYIHN+NCGPG
Sbjct: 301 LVHDFNVSSPGDSLNTDGIHLQNSKDVLIYSSTLSCGDDCISIQTGCSNVYIHNVNCGPG 360

Query: 361 HGISIGSLGKDNTKACVSNITVRDVFMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVK 420
           HGISIGSLGKDNTKACVSNITVRDV MHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVK
Sbjct: 361 HGISIGSLGKDNTKACVSNITVRDVTMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVK 420

Query: 421 IPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLSAIE 452
           IPIVIDQFYCDKAKCSNQT+AVALSGINYERIRGTYTVKPVHFACSDNLPCTDISL+AIE
Sbjct: 421 IPIVIDQFYCDKAKCSNQTAAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLTAIE 480

BLAST of Spg026113 vs. ExPASy TrEMBL
Match: A0A5A7SYF1 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G003070 PE=3 SV=1)

HSP 1 Score: 801.6 bits (2069), Expect = 1.7e-228
Identity = 413/561 (73.62%), Postives = 424/561 (75.58%), Query Frame = 0

Query: 1   MYMKMKGFSFRGLTFSFVVAFLVWSSSLESCHGRTTGKH-WRQNRAASAALSSKKGKAHG 60
           MYMKM+G SFRGLTF F VAFLVWSSSLE CHGR   KH WRQNR AS AL SKKGKAH 
Sbjct: 1   MYMKMEGISFRGLTFMFAVAFLVWSSSLEGCHGRVRSKHYWRQNRVASPALYSKKGKAHS 60

Query: 61  HGGAHKGNHHGGATKTKP-PSRKTLPPLQPPTTKPKPDVPRSPPPQKGGHSSMFNVLDFG 120
           HGG+HKGNHHG A+KTKP PS KT  PL  P TKPKP++PRSPPPQKGGHS MFNVLDFG
Sbjct: 61  HGGSHKGNHHGSASKTKPRPSHKTPQPLPRPVTKPKPNIPRSPPPQKGGHSFMFNVLDFG 120

Query: 121 AKGDGVTDDTK-------------------------------AFQDTWAATCKVAASTCI 180
           AKGDG TDDTK                               AFQDTW A+CKVAAST I
Sbjct: 121 AKGDGKTDDTKYVWWKWYFTGKGNGKGGVGPSRKAESRYGSWAFQDTWTASCKVAASTFI 180

Query: 181 VPSNYVFLVGPISFSGPYCQPDIVFQ---------------------------------- 240
           VPS YVFLVGPISFSGPYCQPDIVFQ                                  
Sbjct: 181 VPSKYVFLVGPISFSGPYCQPDIVFQLDGTIIAPTDFKVWGKGLLQWLQFTKLMGLTIQG 240

Query: 241 -------------------------------------------ARNELVGKMPGIKPTAL 300
                                                      AR+ELVGKMP IKPTAL
Sbjct: 241 NGVIDGRGSVWWQEISPYDDPIDDEFKLLIPLNSTVQEKPPAPARSELVGKMPRIKPTAL 300

Query: 301 RFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVHDFNVSSPGDSLNTDGIHLQNSKDVLI 360
           RFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVHDFNVSSPGDSLNTDGIHLQNSKDVLI
Sbjct: 301 RFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVHDFNVSSPGDSLNTDGIHLQNSKDVLI 360

Query: 361 YSTTLSCGDDCISIQTGCSNVYIHNINCGPGHGISIGSLGKDNTKACVSNITVRDVFMHN 420
           YS+TLSCGDDCISIQTGCSNVYIHN+NCGPGHGISIGSLGKDNTKACVSNITVRDV MHN
Sbjct: 361 YSSTLSCGDDCISIQTGCSNVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHN 420

Query: 421 TMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINY 452
           TMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINY
Sbjct: 421 TMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINY 480

BLAST of Spg026113 vs. TAIR 10
Match: AT1G60590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 582.0 bits (1499), Expect = 4.1e-166
Identity = 301/542 (55.54%), Postives = 360/542 (66.42%), Query Frame = 0

Query: 6   KGFSFRGLTFSFVVAFLVWSSSLESCHGRTTGKHWRQNRAASA----ALSSKKGKAHGHG 65
           +  + R +T   +V  LVWS++LE+C  R  G+HWR +  +S+    +LSSKK K     
Sbjct: 5   ESLTLRSITMMTLVTILVWSATLETCIAR-RGRHWRHHHRSSSSLSDSLSSKKPK----- 64

Query: 66  GAHKGNHHGGATKTKP-PSRKTLPP---------LQPPTTKPKPDVPRSPPP-----QKG 125
            +H+ NHH  + K+KP P  KT PP          QPP  +  P     PPP     Q  
Sbjct: 65  -SHENNHHHMSHKSKPKPKMKTQPPKSNDGSPVVSQPPQVQQPPPPHVQPPPTPLPLQPV 124

Query: 126 GHSSMFNVLDFGAKGDGVTDDTKAFQDTWAATCKVAASTCIVPSNYVFLVGPISFSGPYC 185
             S  FNVLDFGAKGDG++DDT+AF+  WA+ CKV AST I+P +Y+FLVGPISFSGPYC
Sbjct: 125 EDSQQFNVLDFGAKGDGMSDDTQAFEAAWASACKVEASTMIIPPDYIFLVGPISFSGPYC 184

Query: 186 QPDIVFQ----------------------------------------------------- 245
           Q +IVFQ                                                     
Sbjct: 185 QANIVFQLEGMIVAPTDTESWGGGLMWWIEFTKLSGITIQGNGVIDGRGTVWWQQDYLSD 244

Query: 246 ------------------------ARNELVGKMPGIKPTALRFYGSFNVTVTGITIQNSP 305
                                    R+EL  +MP IKPTALRFYGS +VTVTGITIQNSP
Sbjct: 245 YPIDDDFKLIVPLNNSVQERPPMPIRSELNWRMPSIKPTALRFYGSIDVTVTGITIQNSP 304

Query: 306 QCHLKFDNCIGVLVHDFNVSSPGDSLNTDGIHLQNSKDVLIYSTTLSCGDDCISIQTGCS 365
           QCHLKFDNC+ VLVHD NVSSPGDS NTDGIHLQN++DV+I++TTL+CGDDCISIQTGCS
Sbjct: 305 QCHLKFDNCVKVLVHDVNVSSPGDSPNTDGIHLQNTRDVMIHTTTLACGDDCISIQTGCS 364

Query: 366 NVYIHNINCGPGHGISIGSLGKDNTKACVSNITVRDVFMHNTMNGVRIKTWQGGLGFVQG 425
           NVY+HN+NCGPGHGISIGSLGKD+TKACVSNITVRDV MHNTM GVRIKTWQGG+G V+G
Sbjct: 365 NVYVHNVNCGPGHGISIGSLGKDSTKACVSNITVRDVVMHNTMTGVRIKTWQGGIGSVKG 424

Query: 426 VLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDN 452
           +LFSNIQ++EV++PIVIDQFYCD +KC N TSAV++ G+ YE+IRGTYTVKPVHFACSD+
Sbjct: 425 ILFSNIQLTEVQLPIVIDQFYCDHSKCMNHTSAVSVEGVTYEKIRGTYTVKPVHFACSDS 484

BLAST of Spg026113 vs. TAIR 10
Match: AT1G10640.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 580.9 bits (1496), Expect = 9.1e-166
Identity = 298/531 (56.12%), Postives = 353/531 (66.48%), Query Frame = 0

Query: 6   KGFSFRGLTFSFVVAFLVWSSSLESCHGRTTGKHWRQNRAASA----ALSSKKGKAHG-- 65
           +  S R +T   ++A LVWS +LE+C  R  G+HWR +  +S+    +LS+KK K+HG  
Sbjct: 5   RSLSLRSITMMILMAVLVWSVTLETCIAR-RGRHWRHSHRSSSDLSDSLSNKKPKSHGNS 64

Query: 66  -HGGAHKGNHHGGATKTKPPSRKTLPPL----QPPTTKPKPDVPRSPPPQKGGHSSMFNV 125
            H  +H  NHH  +     P  KT P       P  + P    P   PPQKG  S +FNV
Sbjct: 65  HHHSSHNNNHHHKSKPKPKPKLKTPPKAHDNNSPVVSGPPKVQPPPLPPQKG--SQVFNV 124

Query: 126 LDFGAKGDGVTDDTKAFQDTWAATCKVAASTCIVPSNYVFLVGPISFSGPYCQPDIVFQ- 185
           +DFGAKGDG  DDTKAF+  W A CK+ AS  +VP  Y +LVGPISFSGPYCQ +IVFQ 
Sbjct: 125 MDFGAKGDGKCDDTKAFEAAWVAACKIEASVMLVPPEYTYLVGPISFSGPYCQANIVFQL 184

Query: 186 ------------------------------------------------------------ 245
                                                                       
Sbjct: 185 DGTIIAPTDSKTWGKGLMWWIDFTKLKGIKVQGKGVIDGRGSGWWQQDSPFIDSDTKLIV 244

Query: 246 -------------ARNELVGKMPGIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIG 305
                         R+EL  +MP IKPTALRF GSF V VTGITIQNSPQCHLKFD+C+G
Sbjct: 245 PLNNSANQNPPMPIRSELDERMPSIKPTALRFSGSFGVEVTGITIQNSPQCHLKFDDCVG 304

Query: 306 VLVHDFNVSSPGDSLNTDGIHLQNSKDVLIYSTTLSCGDDCISIQTGCSNVYIHNINCGP 365
           V+VHD  VSSPGDS NTDGIHLQN+KDVLI+STTL+CGDDCISIQTGCSNV++HN+NCGP
Sbjct: 305 VVVHDIAVSSPGDSPNTDGIHLQNTKDVLIHSTTLACGDDCISIQTGCSNVFVHNVNCGP 364

Query: 366 GHGISIGSLGKDNTKACVSNITVRDVFMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEV 425
           GHGISIGSLGK+ TKACVSNITVRDV MHNTM GVRIKTWQGG+G V+G++FSNIQ+++V
Sbjct: 365 GHGISIGSLGKEGTKACVSNITVRDVAMHNTMTGVRIKTWQGGVGSVKGIIFSNIQLNQV 424

Query: 426 KIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLSAI 452
           +IPI I+QFYCD +KC NQTSAVA+ G+ YERI+GTYTVKPVHFACSDN PC D+ LS+I
Sbjct: 425 QIPITINQFYCDHSKCKNQTSAVAVEGVTYERIKGTYTVKPVHFACSDNFPCVDVQLSSI 484

BLAST of Spg026113 vs. TAIR 10
Match: AT5G14650.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 462.6 bits (1189), Expect = 3.6e-130
Identity = 231/392 (58.93%), Postives = 266/392 (67.86%), Query Frame = 0

Query: 105 KGGHSSMFNVLDFGAKGDGVTDDTKAFQDTWAATCKVAASTCIVPSNYVFLVGPISFSGP 164
           +GG S   NVLD GAKGDG +DDTKAF+D W   CKVAAST +VPS   FLVGP+SF G 
Sbjct: 44  EGGSSGTINVLDHGAKGDGTSDDTKAFEDAWQVACKVAASTLLVPSGSTFLVGPVSFLGK 103

Query: 165 YCQPDIVFQAR--------------------------------------------NELVG 224
            C+  IVFQ                                              N+++G
Sbjct: 104 ECKEKIVFQLEGKIIAPTSASAWGSGLLQWIEFKALQGITIKGKGIIDGRGSVWWNDMMG 163

Query: 225 -KMPGIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVHDFNVSSPGDSLNTDG 284
            KMP  KPTALRFYGS  VTV+GITIQNSPQ HLKFDNCI + V DF  SSPGDS NTDG
Sbjct: 164 TKMPRTKPTALRFYGSNGVTVSGITIQNSPQTHLKFDNCISIQVSDFTTSSPGDSPNTDG 223

Query: 285 IHLQNSKDVLIYSTTLSCGDDCISIQTGCSNVYIHNINCGPGHGISIGSLGKDNTKACVS 344
           IHLQNS+D +IY +TL+CGDDCISIQTGCSN+ IH+++CGPGHGISIG LGKDNTKACVS
Sbjct: 224 IHLQNSQDAVIYRSTLACGDDCISIQTGCSNINIHDVDCGPGHGISIGGLGKDNTKACVS 283

Query: 345 NITVRDVFMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIVIDQFYCDKAKCSNQ 404
           NITVRDV MH T NGVRIK+WQGG G V+ V+FSNIQVS V  PI+IDQ+YCD   C N+
Sbjct: 284 NITVRDVTMHETTNGVRIKSWQGGSGSVKQVMFSNIQVSNVANPIIIDQYYCDGGGCHNE 343

Query: 405 TSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLSAIELKPLQELYHLYGAFCWQT 452
           TSAVA+S INY  I+GTYT +PV FACSD+LPCT ISLS IELKP          FCW+ 
Sbjct: 344 TSAVAVSNINYINIKGTYTKEPVRFACSDSLPCTGISLSTIELKPATGKASSLDPFCWKA 403

BLAST of Spg026113 vs. TAIR 10
Match: AT3G26610.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 354.8 bits (909), Expect = 1.1e-97
Identity = 183/406 (45.07%), Postives = 242/406 (59.61%), Query Frame = 0

Query: 81  KTLPPLQPPTTKPKPDVPRSPPPQKGGHSSMFNVLDFGAKGDGVTDDTKAFQDTWAATCK 140
           K + P     +K  P    +P P    +  +FN+  +GAKGDGV+DD+KA    W A CK
Sbjct: 36  KKIKPKHRRHSKNTPTGSPAPAPYPSTNEGVFNIFSYGAKGDGVSDDSKALVGAWKAACK 95

Query: 141 VAASTCIVPSNYVFLVGPISFSGPYCQPDIVFQARNELVGK------------------- 200
           V      +P+   FLV  ++  GP C+ + V Q    LV                     
Sbjct: 96  VVGGKVEIPAGTQFLVKAVTLQGP-CKEETVVQIEGILVAPEKIGSWPNSSLFQWLNFKW 155

Query: 201 ---------------------------------MPGIKPTALRFYGSFNVTVTGITIQNS 260
                                            +P +KPTALRFY S NVTV  I+I NS
Sbjct: 156 VSHLTIQGSGTLNGRGYNWWNLDTYQTQTRNKYIPPMKPTALRFYSSNNVTVRDISIVNS 215

Query: 261 PQCHLKFDNCIGVLVHDFNVSSPGDSLNTDGIHLQNSKDVLIYSTTLSCGDDCISIQTGC 320
           P CHLKFD+  GV V++  +SSP +S NTDGIHLQN+++V I  + ++CGDDC+SIQTG 
Sbjct: 216 PLCHLKFDDSDGVKVNNITISSPENSPNTDGIHLQNTRNVEIQHSNIACGDDCVSIQTGS 275

Query: 321 SNVYIHNINCGPGHGISIGSLGKDNTKACVSNITVRDVFMHNTMNGVRIKTWQGGLGFVQ 380
           SNV+IH+INCGPGHGISIG LGKD + ACVS+I V D+ + NT+ GVRIKTWQGGLG V+
Sbjct: 276 SNVHIHHINCGPGHGISIGGLGKDKSVACVSDIIVEDISIQNTLAGVRIKTWQGGLGVVK 335

Query: 381 GVLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSD 430
            + FSNIQV +VK+PIVIDQ+YCDK+KC NQT AV++SG+ Y  I G++TV+PV  ACS+
Sbjct: 336 NLTFSNIQVKDVKVPIVIDQYYCDKSKCKNQTRAVSISGVKYNNIVGSFTVQPVRIACSN 395

BLAST of Spg026113 vs. TAIR 10
Match: AT1G48100.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 332.8 bits (852), Expect = 4.3e-91
Identity = 189/439 (43.05%), Postives = 243/439 (55.35%), Query Frame = 0

Query: 64  HKGNHHGGATKTKPPSRKTLPPLQ---PPTTKPKPDVPRSPPPQKGGHSS--MFNVLDFG 123
           HKG H  G T   PP     PPL+   PP   P    P SP P  G   S  +F+V  FG
Sbjct: 32  HKGRH--GVTHPLPPPPP--PPLETANPPDQVPSDPYP-SPDPAPGDSDSGCVFDVTSFG 91

Query: 124 AKGDGVTDDTKAFQDTWAATCKVAASTCIVPSNYVFLVGPISFSGPYCQPDIVFQARNEL 183
           A GDG  DDT AFQD W A C V +   + P   VF +    FSGP C+P +VFQ    L
Sbjct: 92  AVGDGSCDDTAAFQDAWKAACAVESGVVLAPEGGVFKITSTIFSGP-CKPGLVFQLDGVL 151

Query: 184 V--------------------------------------------------------GKM 243
           +                                                           
Sbjct: 152 MPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQKWWDLPCKPHRGPDGSSSSG 211

Query: 244 PGIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVHDFNVSSPGDSLNTDGIHL 303
           P   PT +RF+ S N+ V G+ IQNSPQ H+KFD C GVL+++  +SSP  S NTDGIHL
Sbjct: 212 PCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGVLINEIQISSPKLSPNTDGIHL 271

Query: 304 QNSKDVLIYSTTLSCGDDCISIQTGCSNVYIHNINCGPGHGISIGSLGKDNTKACVSNIT 363
            N++ V IY++ +S GDDCISI TGCS+V I  + CGP HGISIGSLG  N++ACVSNIT
Sbjct: 272 GNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPSHGISIGSLGVHNSQACVSNIT 331

Query: 364 VRDVFMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSA 423
           VR+  + ++ NG+R+KTWQGG G V  +LF NIQ+  V   I++DQ+YC    C N+TSA
Sbjct: 332 VRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMENVLNCIIVDQYYCQSKDCRNETSA 391

Query: 424 VALSGINYERIRGTYTVK--PVHFACSDNLPCTDISLSAIELKPLQELYHLYGAFCWQTF 440
           V +  + Y  I+GTY V+  P+HFACSD + CT+I++S +EL P +E   +   FCW  +
Sbjct: 392 VKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITMSEVELLP-EEGELVDDPFCWNAY 451

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008446197.14.7e-23377.92PREDICTED: polygalacturonase At1g48100-like [Cucumis melo] >XP_016900181.1 PREDI... [more]
XP_038893387.11.1e-23277.88polygalacturonase At1g48100-like isoform X2 [Benincasa hispida][more]
XP_038893386.17.5e-23177.15polygalacturonase At1g48100-like isoform X1 [Benincasa hispida][more]
XP_004135250.12.4e-22976.79polygalacturonase At1g48100 [Cucumis sativus] >KGN51797.1 hypothetical protein C... [more]
KAA0034307.13.5e-22873.62polygalacturonase [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q949Z16.0e-9043.05Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV... [more]
Q94AJ58.8e-6537.03Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017... [more]
Q7M1E71.1e-6238.90Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
P432121.4e-6237.86Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1[more]
Q8RY292.9e-6038.71Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A1S3BFB42.3e-23377.92polygalacturonase At1g48100-like OS=Cucumis melo OX=3656 GN=LOC103489001 PE=3 SV... [more]
A0A5D3CUP52.3e-23377.92Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001... [more]
E5GBI62.3e-23377.92Polygalacturonase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1[more]
A0A0A0KTN91.2e-22976.79Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G601470 PE=3 SV=1[more]
A0A5A7SYF11.7e-22873.62Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G003... [more]
Match NameE-valueIdentityDescription
AT1G60590.14.1e-16655.54Pectin lyase-like superfamily protein [more]
AT1G10640.19.1e-16656.12Pectin lyase-like superfamily protein [more]
AT5G14650.13.6e-13058.93Pectin lyase-like superfamily protein [more]
AT3G26610.11.1e-9745.07Pectin lyase-like superfamily protein [more]
AT1G48100.14.3e-9143.05Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 298..319
e-value: 220.0
score: 10.6
coord: 268..288
e-value: 4900.0
score: 2.3
coord: 195..244
e-value: 200.0
score: 10.8
coord: 327..348
e-value: 7400.0
score: 1.0
coord: 245..266
e-value: 950.0
score: 7.4
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 185..403
e-value: 6.1E-67
score: 226.0
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 275..288
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 101..433
e-value: 2.1E-96
score: 325.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 32..112
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 57..75
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 81..102
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 182..441
coord: 10..174
NoneNo IPR availablePANTHERPTHR31375:SF192PECTIN LYASE-LIKE SUPERFAMILY PROTEINcoord: 182..441
coord: 10..174
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 109..446

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg026113.1Spg026113.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity