Spg025963 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg025963
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	EF-hand domain-containing protein
Location	scaffold7: 591559 .. 593223 (+)
RNA-Seq Expression	Spg025963
Synteny	Spg025963

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTGGCCTCTTAACGGTAACTTCTTTGAAGTTTCTAGAGTTTGATTTAAAATAATATATATTGTCTCCATATCTTTGTTACTTTTCTTTTTTGCCATCAGTAAGTTGATTTAAAAACTGAACATATGATGCCTCGCATTTCAGGTAAATCAAAGTTTTTAATTAACCGATATTTTAGCCAATTGAGATTTTTTCCCTTCTCGTGTGTTAGTGGTTGAATGATTTAGTCGGTTAAGTTTTTTCAGGTTAGTGGGCAATAAAGTAGCGTTTTTTTTTTCTCCCAGTCGCACCGCCGCCTCCTCGCGCTGCCGGTCTCATCTTGTGCCGCCGCCACCGTCGCCCTCGTCTCCCTCTCCCTCGATTTTTCTTTCTCCAGCAACAAGGAAGCCGTGGGTCTCAATCCTTTTCAGATTGCTGCCATAGTATAGTAACATGTTAGTTATTTTTTATTTTTTATTTTTAAAGAGTAACATGTTAGTTATTCACTTTCTGAAAAGGATTCTTTTGCATTTATGAAGGGGGACTAGAATAGTCGAATTGATGTAATAAAATAATCAATCAATGGAAAATTACTCCCAAAGAAAGAGAATGTAAAACAACAAAGTGAATAAAAGCAGGCGGATAAGTAATTATTTTGGTTGACCTAGCCTTGTTCTAACATAAATGATTTTCTGTCTAGAACAAAGAAAGAAGAAGAATTATTTGTGACCTCTCATATCTAATTCTGTAATCACTAGTTGTTTCAGAACCAGCTCCGAAACGTTTCACTGTCTGAAAAGGACGCTTTTGCATTTCTTAAGGGTGAAATAAATGATGATATAATTAGATACTCAACTTTTTGTGAAGTACTTCGCCAAGTATTTTAGATCCCAGCTAATTCCTTTCTGCAGATATTTGAACGGAGATATTAAATATAACTAATTTTGTCTTCAACCTCACACAGGTAAATTTGATTGATCGCCCTTATGGGTTGAGTTTTCAAGAAATGGAGAATCTGTGGACACAAGCAGACATTGATGGAAACGGTGCAGTCAGTTATGAAGAATTTCTGGTATGTTCATCACATTTCCTTGTTTGTGCGGTGACTATTGGAAACAATCAGCCAACGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTCACAGTCTATGATGTCTATGAAATCTTTTGGCTTACCTACAAGGTTAATGTTTATAAGCCACGAAATGATTGTATAGCATCGAAAGCAGTTTGATTTTGAATATTTATCATTTCATTTTGGCTTCCCAGTCTATAGATGTGATTCTTTAGCGAGGGACGATTACTTGTCTAAGCTTTGGCATGACTTTATTCCCTCCTTGGCCTTGTCTTCTTCCAATTCCTTAATTTCACATGTGGAACTTGTATGCAAAACCTGTACGCAAGGTGGTAATCTGCTCAAATCTAAACATGCAGCAGAGGCTATGGAGTTATACATGTTCCGAACAGCGAGAGGAAAATGCGAATGGGAGAACTGGGGGCTCTGATGAGTGCAACGAGGAAGAAGTTGTAGGTTTCAAAGTGAAGCATCTCGTTCTATTTCCTCGGGAAGTTGAGAAAGGGATGTGGCCTGATGGTTATTCCCTCTCCGATCATGCCCGACTTACAGTCGTGTTTTCACCAGTAATGCTGCAGTATTCTTAG

mRNA sequence

ATGCTTGGCCTCTTAACGAACCAGCTCCGAAACGTTTCACTGTCTGAAAAGGACGCTTTTGCATTTCTTAAGGGTGAAATAAATGATGATATAATTAGATACTCAACTTTTTGTGAAGTACTTCGCCAAGTAAATTTGATTGATCGCCCTTATGGGTTGAGTTTTCAAGAAATGGAGAATCTGTGGACACAAGCAGACATTGATGGAAACGGTGCAGTCAGTTATGAAGAATTTCTGCAGAGGCTATGGAGTTATACATGTTCCGAACAGCGAGAGGAAAATGCGAATGGGAGAACTGGGGGCTCTGATGAGTGCAACGAGGAAGAAGTTGTAGGTTTCAAAGTGAAGCATCTCGTTCTATTTCCTCGGGAAGTTGAGAAAGGGATGTGGCCTGATGGTTATTCCCTCTCCGATCATGCCCGACTTACAGTCGTGTTTTCACCAGTAATGCTGCAGTATTCTTAG

Coding sequence (CDS)

ATGCTTGGCCTCTTAACGAACCAGCTCCGAAACGTTTCACTGTCTGAAAAGGACGCTTTTGCATTTCTTAAGGGTGAAATAAATGATGATATAATTAGATACTCAACTTTTTGTGAAGTACTTCGCCAAGTAAATTTGATTGATCGCCCTTATGGGTTGAGTTTTCAAGAAATGGAGAATCTGTGGACACAAGCAGACATTGATGGAAACGGTGCAGTCAGTTATGAAGAATTTCTGCAGAGGCTATGGAGTTATACATGTTCCGAACAGCGAGAGGAAAATGCGAATGGGAGAACTGGGGGCTCTGATGAGTGCAACGAGGAAGAAGTTGTAGGTTTCAAAGTGAAGCATCTCGTTCTATTTCCTCGGGAAGTTGAGAAAGGGATGTGGCCTGATGGTTATTCCCTCTCCGATCATGCCCGACTTACAGTCGTGTTTTCACCAGTAATGCTGCAGTATTCTTAG

Protein sequence

MLGLLTNQLRNVSLSEKDAFAFLKGEINDDIIRYSTFCEVLRQVNLIDRPYGLSFQEMENLWTQADIDGNGAVSYEEFLQRLWSYTCSEQREENANGRTGGSDECNEEEVVGFKVKHLVLFPREVEKGMWPDGYSLSDHARLTVVFSPVMLQYS

Homology

[Close]

BLAST of Spg025963 vs. NCBI nr
Match: XP_023521956.1 (uncharacterized calcium-binding protein At1g02270-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 226.9 bits (577), Expect = 1.2e-55
Identity = 114/154 (74.03%), Postives = 122/154 (79.22%), Query Frame = 0


Query: 1   MLGLLTNQLRNVSLSEKDAFAFLKGEINDDIIRYSTFCEVLRQVNLIDRPYGLSFQEMEN 60
           + G++ NQL  VS+SEKD       E NDD+IRYST CE LRQVNLID PYGLSFQE +N
Sbjct: 275 VFGIIKNQLGKVSVSEKD-------ETNDDLIRYSTLCEALRQVNLIDLPYGLSFQETKN 334

Query: 61  LWTQADIDGNGAVSYEEFLQRLWSYTCSEQREENANGRTGGSDECNEEEVVGFKVKHLVL 120
           LWTQA IDGNG  SYE+FLQRL S+ CSEQ EEN NGRT   D CNEEEVVGFKVKH VL
Sbjct: 335 LWTQAAIDGNGVASYEQFLQRLRSFACSEQTEENVNGRT---DACNEEEVVGFKVKHAVL 394

Query: 121 FPREVEKGMWPDGYSLSDHARLTVVFSPVMLQYS 155
           FPREVEKG WPDGYSLSDHARL V FSPVMLQ S
Sbjct: 395 FPREVEKGTWPDGYSLSDHARLRVEFSPVMLQCS 418

[Close]

BLAST of Spg025963 vs. NCBI nr
Match: KAG7028468.1 (putative calcium-binding protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 225.7 bits (574), Expect = 2.7e-55
Identity = 113/154 (73.38%), Postives = 122/154 (79.22%), Query Frame = 0


Query: 1   MLGLLTNQLRNVSLSEKDAFAFLKGEINDDIIRYSTFCEVLRQVNLIDRPYGLSFQEMEN 60
           + G++ NQ+  VS+SEKD       E NDD+IRYST CE LRQVNLID PYGLSFQE +N
Sbjct: 275 VFGIIKNQVGKVSVSEKD-------ETNDDLIRYSTLCEALRQVNLIDLPYGLSFQETKN 334

Query: 61  LWTQADIDGNGAVSYEEFLQRLWSYTCSEQREENANGRTGGSDECNEEEVVGFKVKHLVL 120
           LWTQA IDGNG  SYE+FLQRL S+ CSEQ EEN NGRT   D CNEEEVVGFKVKH VL
Sbjct: 335 LWTQAAIDGNGVASYEQFLQRLRSFACSEQTEENVNGRT---DACNEEEVVGFKVKHAVL 394

Query: 121 FPREVEKGMWPDGYSLSDHARLTVVFSPVMLQYS 155
           FPREVEKG WPDGYSLSDHARL V FSPVMLQ S
Sbjct: 395 FPREVEKGTWPDGYSLSDHARLRVEFSPVMLQCS 418

[Close]

BLAST of Spg025963 vs. NCBI nr
Match: KAG6597001.1 (putative calcium-binding protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 225.7 bits (574), Expect = 2.7e-55
Identity = 113/154 (73.38%), Postives = 122/154 (79.22%), Query Frame = 0


Query: 1   MLGLLTNQLRNVSLSEKDAFAFLKGEINDDIIRYSTFCEVLRQVNLIDRPYGLSFQEMEN 60
           + G++ NQ+  VS+SEKD       E NDD+IRYST CE LRQVNLID PYGLSFQE +N
Sbjct: 642 VFGIIKNQVGKVSVSEKD-------ETNDDLIRYSTLCEALRQVNLIDLPYGLSFQETKN 701

Query: 61  LWTQADIDGNGAVSYEEFLQRLWSYTCSEQREENANGRTGGSDECNEEEVVGFKVKHLVL 120
           LWTQA IDGNG  SYE+FLQRL S+ CSEQ EEN NGRT   D CNEEEVVGFKVKH VL
Sbjct: 702 LWTQAAIDGNGVASYEQFLQRLRSFACSEQTEENVNGRT---DACNEEEVVGFKVKHAVL 761

Query: 121 FPREVEKGMWPDGYSLSDHARLTVVFSPVMLQYS 155
           FPREVEKG WPDGYSLSDHARL V FSPVMLQ S
Sbjct: 762 FPREVEKGTWPDGYSLSDHARLRVEFSPVMLQCS 785

[Close]

BLAST of Spg025963 vs. NCBI nr
Match: XP_023538954.1 (uncharacterized calcium-binding protein At1g02270-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 223.8 bits (569), Expect = 1.0e-54
Identity = 113/154 (73.38%), Postives = 121/154 (78.57%), Query Frame = 0


Query: 1   MLGLLTNQLRNVSLSEKDAFAFLKGEINDDIIRYSTFCEVLRQVNLIDRPYGLSFQEMEN 60
           + G++ NQL  VS+SEKD       E NDD+IRYST CE LRQVNLID PYGLSFQE +N
Sbjct: 275 VFGIIKNQLGKVSVSEKD-------ETNDDLIRYSTLCEALRQVNLIDLPYGLSFQETKN 334

Query: 61  LWTQADIDGNGAVSYEEFLQRLWSYTCSEQREENANGRTGGSDECNEEEVVGFKVKHLVL 120
           LWTQA IDGNG  SYE+FLQRL S+ CSEQ EEN NGRT   D CNEEEVVGFKVKH VL
Sbjct: 335 LWTQAAIDGNGVASYEQFLQRLRSFACSEQTEENVNGRT---DACNEEEVVGFKVKHAVL 394

Query: 121 FPREVEKGMWPDGYSLSDHARLTVVFSPVMLQYS 155
           FPREVE G WPDGYSLSDHARL V FSPVMLQ S
Sbjct: 395 FPREVEIGTWPDGYSLSDHARLRVEFSPVMLQCS 418

[Close]

BLAST of Spg025963 vs. NCBI nr
Match: XP_023521440.1 (uncharacterized calcium-binding protein At1g02270-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 223.8 bits (569), Expect = 1.0e-54
Identity = 113/154 (73.38%), Postives = 121/154 (78.57%), Query Frame = 0


Query: 1   MLGLLTNQLRNVSLSEKDAFAFLKGEINDDIIRYSTFCEVLRQVNLIDRPYGLSFQEMEN 60
           + G++ NQL  VS+SEKD       E NDD+IRYST CE LRQVNLID PYGLSFQE +N
Sbjct: 14  VFGIIKNQLGKVSVSEKD-------ETNDDLIRYSTLCEALRQVNLIDLPYGLSFQETKN 73

Query: 61  LWTQADIDGNGAVSYEEFLQRLWSYTCSEQREENANGRTGGSDECNEEEVVGFKVKHLVL 120
           LWTQA IDGNG  SYE+FLQRL S+ CSEQ EEN NGRT   D CNEEEVVGFKVKH VL
Sbjct: 74  LWTQAAIDGNGVASYEQFLQRLRSFACSEQTEENVNGRT---DACNEEEVVGFKVKHAVL 133

Query: 121 FPREVEKGMWPDGYSLSDHARLTVVFSPVMLQYS 155
           FPREVE G WPDGYSLSDHARL V FSPVMLQ S
Sbjct: 134 FPREVEIGTWPDGYSLSDHARLRVEFSPVMLQCS 157

[Close]

BLAST of Spg025963 vs. ExPASy Swiss-Prot
Match: O81916 (Uncharacterized calcium-binding protein At1g02270 OS=Arabidopsis thaliana OX=3702 GN=At1g02270 PE=2 SV=2)

HSP 1 Score: 140.6 bits (353), Expect = 1.5e-32
Identity = 66/151 (43.71%), Postives = 94/151 (62.25%), Query Frame = 0


Query: 1   MLGLLTNQLRNVSLSEKDAFAFLKGEINDDIIRYSTFCEVLRQVNLIDRPYGLSFQEMEN 60
           +  ++  QL   S++E DAF FL  + + D + YS FC  L++VNL   P+GLSF+E + 
Sbjct: 332 VFSIIKYQLHKASIAEDDAFTFLGAKNHSDSLTYSDFCLALQKVNLTGIPHGLSFEETKE 391

Query: 61  LWTQADIDGNGAVSYEEFLQRLWSYTCSEQREENANGRTGGSDECNEEEVVGFKVKHLVL 120
           LW +AD+DGNG   YEE L+++W+ T   Q             E  E+E +G KV   +L
Sbjct: 392 LWVRADLDGNGVFDYEE-LKKIWNMTMVNQPGNCKESVMESKKEEGEDEAIGLKVNKAIL 451

Query: 121 FPREVEKGMWPDGYSLSDHARLTVVFSPVML 152
           FP+E EKG+WP+ Y++SDHA LTV FSPV +
Sbjct: 452 FPQEAEKGLWPENYNISDHACLTVQFSPVKM 481

[Close]

BLAST of Spg025963 vs. ExPASy TrEMBL
Match: A0A6J1FA14 (uncharacterized calcium-binding protein At1g02270-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442171 PE=4 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 8.5e-55
Identity = 112/154 (72.73%), Postives = 121/154 (78.57%), Query Frame = 0


Query: 1   MLGLLTNQLRNVSLSEKDAFAFLKGEINDDIIRYSTFCEVLRQVNLIDRPYGLSFQEMEN 60
           + G++ NQ+  VS+SEKD       E NDD+IRYST CE LRQVNLID PYGLSFQE +N
Sbjct: 275 VFGIIKNQVGKVSVSEKD-------ETNDDLIRYSTLCEALRQVNLIDLPYGLSFQETKN 334

Query: 61  LWTQADIDGNGAVSYEEFLQRLWSYTCSEQREENANGRTGGSDECNEEEVVGFKVKHLVL 120
           LWTQA IDGNG  SYE+FLQRL S+ CSEQ EEN NGRT   D CNEE VVGFKVKH VL
Sbjct: 335 LWTQAAIDGNGVASYEQFLQRLRSFACSEQTEENVNGRT---DACNEEVVVGFKVKHAVL 394

Query: 121 FPREVEKGMWPDGYSLSDHARLTVVFSPVMLQYS 155
           FPREVEKG WPDGYSLSDHARL V FSPVMLQ S
Sbjct: 395 FPREVEKGTWPDGYSLSDHARLRVEFSPVMLQCS 418

[Close]

BLAST of Spg025963 vs. ExPASy TrEMBL
Match: A0A6J1IFR0 (uncharacterized calcium-binding protein At1g02270-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111472911 PE=4 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 3.6e-53
Identity = 112/154 (72.73%), Postives = 119/154 (77.27%), Query Frame = 0


Query: 1   MLGLLTNQLRNVSLSEKDAFAFLKGEINDDIIRYSTFCEVLRQVNLIDRPYGLSFQEMEN 60
           + G++ NQL  VS+SEKD       E NDD+IRYST CE LRQVNLID PYGLSFQE +N
Sbjct: 275 VFGIIKNQLGKVSVSEKD-------ETNDDLIRYSTLCEALRQVNLIDLPYGLSFQETKN 334

Query: 61  LWTQADIDGNGAVSYEEFLQRLWSYTCSEQREENANGRTGGSDECNEEEVVGFKVKHLVL 120
           LWTQA IDGNG  SYE FLQRL S+ CSEQ EEN NGRT   D CN EE VGFKVKH VL
Sbjct: 335 LWTQAAIDGNGVASYEPFLQRLRSFACSEQTEENVNGRT---DACN-EEFVGFKVKHAVL 394

Query: 121 FPREVEKGMWPDGYSLSDHARLTVVFSPVMLQYS 155
           FPREVEKG WPDGYSLSDHARL V FSPVMLQ S
Sbjct: 395 FPREVEKGTWPDGYSLSDHARLRVEFSPVMLQCS 417

[Close]

BLAST of Spg025963 vs. ExPASy TrEMBL
Match: A0A6J1F4U5 (uncharacterized calcium-binding protein At1g02270-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111442171 PE=4 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 8.0e-53
Identity = 111/154 (72.08%), Postives = 120/154 (77.92%), Query Frame = 0


Query: 1   MLGLLTNQLRNVSLSEKDAFAFLKGEINDDIIRYSTFCEVLRQVNLIDRPYGLSFQEMEN 60
           + G++ NQ+  VS+SEKD       E NDD+IRYST CE LRQVNLID PYGLSFQE +N
Sbjct: 275 VFGIIKNQVGKVSVSEKD-------ETNDDLIRYSTLCEALRQVNLIDLPYGLSFQETKN 334

Query: 61  LWTQADIDGNGAVSYEEFLQRLWSYTCSEQREENANGRTGGSDECNEEEVVGFKVKHLVL 120
           LWTQA IDGNG  SYE+FL RL S+ CSEQ EEN NGRT   D CNEE VVGFKVKH VL
Sbjct: 335 LWTQAAIDGNGVASYEQFL-RLRSFACSEQTEENVNGRT---DACNEEVVVGFKVKHAVL 394

Query: 121 FPREVEKGMWPDGYSLSDHARLTVVFSPVMLQYS 155
           FPREVEKG WPDGYSLSDHARL V FSPVMLQ S
Sbjct: 395 FPREVEKGTWPDGYSLSDHARLRVEFSPVMLQCS 417

[Close]

BLAST of Spg025963 vs. ExPASy TrEMBL
Match: A0A6J1IH47 (uncharacterized calcium-binding protein At1g02270-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111472911 PE=4 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 3.3e-51
Identity = 111/154 (72.08%), Postives = 118/154 (76.62%), Query Frame = 0


Query: 1   MLGLLTNQLRNVSLSEKDAFAFLKGEINDDIIRYSTFCEVLRQVNLIDRPYGLSFQEMEN 60
           + G++ NQL  VS+SEKD       E NDD+IRYST CE LRQVNLID PYGLSFQE +N
Sbjct: 275 VFGIIKNQLGKVSVSEKD-------ETNDDLIRYSTLCEALRQVNLIDLPYGLSFQETKN 334

Query: 61  LWTQADIDGNGAVSYEEFLQRLWSYTCSEQREENANGRTGGSDECNEEEVVGFKVKHLVL 120
           LWTQA IDGNG  SYE FL RL S+ CSEQ EEN NGRT   D CN EE VGFKVKH VL
Sbjct: 335 LWTQAAIDGNGVASYEPFL-RLRSFACSEQTEENVNGRT---DACN-EEFVGFKVKHAVL 394

Query: 121 FPREVEKGMWPDGYSLSDHARLTVVFSPVMLQYS 155
           FPREVEKG WPDGYSLSDHARL V FSPVMLQ S
Sbjct: 395 FPREVEKGTWPDGYSLSDHARLRVEFSPVMLQCS 416

[Close]

BLAST of Spg025963 vs. ExPASy TrEMBL
Match: A0A4U5MN34 (Uncharacterized protein OS=Populus alba OX=43335 GN=D5086_0000305740 PE=4 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 5.9e-48
Identity = 96/154 (62.34%), Postives = 113/154 (73.38%), Query Frame = 0


Query: 1   MLGLLTNQLRNVSLSEKDAFAFLKGEINDDIIRYSTFCEVLRQVNLIDRPYGLSFQEMEN 60
           + G++  QL+  SL E DAFAFLK + ND+ I YS FCE LRQVNLI  PYGLS QE E+
Sbjct: 319 VFGIIKCQLQKASLVENDAFAFLKADNNDNFITYSAFCEALRQVNLIGLPYGLSSQETED 378

Query: 61  LWTQADIDGNGAVSYEEFLQRLWSYTCSEQREENANGRTGGSDECNEEEVVGFKVKHLVL 120
           LW Q DI+GNG V YEEF + +W+  CSE REEN + RTG S++  EEE +GF VK  VL
Sbjct: 379 LWMQVDINGNGVVGYEEFKRSIWNSECSEPREENCSERTGDSEQGLEEEAIGFNVKKAVL 438

Query: 121 FPREVEKGMWPDGYSLSDHARLTVVFSPVMLQYS 155
           FPRE EKG WP+ YSLSDHA LTVVFSPV +Q S
Sbjct: 439 FPREAEKGSWPENYSLSDHAPLTVVFSPVRIQGS 472

[Close]

BLAST of Spg025963 vs. TAIR 10
Match: AT5G54130.2 (Calcium-binding endonuclease/exonuclease/phosphatase family )

HSP 1 Score: 168.3 bits (425), Expect = 4.8e-42
Identity = 83/154 (53.90%), Postives = 108/154 (70.13%), Query Frame = 0


Query: 1   MLGLLTNQLRNVSLSEKDAFAFLKGEINDDIIRYSTFCEVLRQVNLIDRPYGLSFQEMEN 60
           + G+    LR  SL+ +DAFAFLK + + D I +  FCE LRQ+NL     GL+ +E+++
Sbjct: 284 VFGMFRYLLRRASLTAEDAFAFLKTDNDGDHITFMGFCETLRQLNLTGHCNGLTTKEIKD 343

Query: 61  LWTQADIDGNGAVSYEEFLQRLWSYTCSEQREENANGRTGGSDECNEEEVVGFKVKHLVL 120
           LWTQADIDGNG + Y+EF QR+W+ T SEQR+        G  + N+E+ VGF VK+ VL
Sbjct: 344 LWTQADIDGNGLLDYKEFQQRIWNQTWSEQRDAE-----DGEAKGNQEQTVGFSVKNAVL 403

Query: 121 FPREVEKGMWPDGYSLSDHARLTVVFSPVMLQYS 155
           FP EVEKGMWP+ YSLSDHARLTVVFSP+ +  S
Sbjct: 404 FPPEVEKGMWPENYSLSDHARLTVVFSPIRMPCS 432

[Close]

BLAST of Spg025963 vs. TAIR 10
Match: AT1G02270.1 (Calcium-binding endonuclease/exonuclease/phosphatase family )

HSP 1 Score: 140.6 bits (353), Expect = 1.1e-33
Identity = 66/151 (43.71%), Postives = 94/151 (62.25%), Query Frame = 0


Query: 1   MLGLLTNQLRNVSLSEKDAFAFLKGEINDDIIRYSTFCEVLRQVNLIDRPYGLSFQEMEN 60
           +  ++  QL   S++E DAF FL  + + D + YS FC  L++VNL   P+GLSF+E + 
Sbjct: 332 VFSIIKYQLHKASIAEDDAFTFLGAKNHSDSLTYSDFCLALQKVNLTGIPHGLSFEETKE 391

Query: 61  LWTQADIDGNGAVSYEEFLQRLWSYTCSEQREENANGRTGGSDECNEEEVVGFKVKHLVL 120
           LW +AD+DGNG   YEE L+++W+ T   Q             E  E+E +G KV   +L
Sbjct: 392 LWVRADLDGNGVFDYEE-LKKIWNMTMVNQPGNCKESVMESKKEEGEDEAIGLKVNKAIL 451

Query: 121 FPREVEKGMWPDGYSLSDHARLTVVFSPVML 152
           FP+E EKG+WP+ Y++SDHA LTV FSPV +
Sbjct: 452 FPQEAEKGLWPENYNISDHACLTVQFSPVKM 481

[Close]

BLAST of Spg025963 vs. TAIR 10
Match: AT5G54130.3 (Calcium-binding endonuclease/exonuclease/phosphatase family )

HSP 1 Score: 45.8 bits (107), Expect = 3.6e-05
Identity = 22/48 (45.83%), Postives = 31/48 (64.58%), Query Frame = 0


Query: 1   MLGLLTNQLRNVSLSEKDAFAFLKGEINDDIIRYSTFCEVLRQVNLID 49
           + G+    LR  SL+ +DAFAFLK + + D I +  FCE LRQV ++D
Sbjct: 282 VFGMFRYLLRRASLTAEDAFAFLKTDNDGDHITFMGFCETLRQVTVMD 329

[Close]

BLAST of Spg025963 vs. TAIR 10
Match: AT5G54130.4 (Calcium-binding endonuclease/exonuclease/phosphatase family )

HSP 1 Score: 45.8 bits (107), Expect = 3.6e-05
Identity = 22/48 (45.83%), Postives = 31/48 (64.58%), Query Frame = 0


Query: 1   MLGLLTNQLRNVSLSEKDAFAFLKGEINDDIIRYSTFCEVLRQVNLID 49
           + G+    LR  SL+ +DAFAFLK + + D I +  FCE LRQV ++D
Sbjct: 284 VFGMFRYLLRRASLTAEDAFAFLKTDNDGDHITFMGFCETLRQVTVMD 331

The following BLAST results are available for this feature:

BLAST of Spg025963 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023521956.1	1.2e-55	74.03	uncharacterized calcium-binding protein At1g02270-like isoform X1 [Cucurbita pep...	[more]
KAG7028468.1	2.7e-55	73.38	putative calcium-binding protein [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6597001.1	2.7e-55	73.38	putative calcium-binding protein, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_023538954.1	1.0e-54	73.38	uncharacterized calcium-binding protein At1g02270-like isoform X1 [Cucurbita pep...	[more]
XP_023521440.1	1.0e-54	73.38	uncharacterized calcium-binding protein At1g02270-like [Cucurbita pepo subsp. pe...	[more]

BLAST of Spg025963 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81916	1.5e-32	43.71	Uncharacterized calcium-binding protein At1g02270 OS=Arabidopsis thaliana OX=370...	[more]

BLAST of Spg025963 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FA14	8.5e-55	72.73	uncharacterized calcium-binding protein At1g02270-like isoform X1 OS=Cucurbita m...	[more]
A0A6J1IFR0	3.6e-53	72.73	uncharacterized calcium-binding protein At1g02270-like isoform X1 OS=Cucurbita m...	[more]
A0A6J1F4U5	8.0e-53	72.08	uncharacterized calcium-binding protein At1g02270-like isoform X2 OS=Cucurbita m...	[more]
A0A6J1IH47	3.3e-51	72.08	uncharacterized calcium-binding protein At1g02270-like isoform X2 OS=Cucurbita m...	[more]
A0A4U5MN34	5.9e-48	62.34	Uncharacterized protein OS=Populus alba OX=43335 GN=D5086_0000305740 PE=4 SV=1	[more]

BLAST of Spg025963 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G54130.2	4.8e-42	53.90	Calcium-binding endonuclease/exonuclease/phosphatase family	[more]
AT1G02270.1	1.1e-33	43.71	Calcium-binding endonuclease/exonuclease/phosphatase family	[more]
AT5G54130.3	3.6e-05	45.83	Calcium-binding endonuclease/exonuclease/phosphatase family	[more]
AT5G54130.4	3.6e-05	45.83	Calcium-binding endonuclease/exonuclease/phosphatase family	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.10.238.10		coord: 14..82 e-value: 9.5E-7 score: 30.6
None	No IPR available	PANTHER	PTHR12121:SF50	ENDONUCLEASE/EXONUCLEASE/PHOSPHATASE FAMILY PROTEIN	coord: 9..152
None	No IPR available	PANTHER	PTHR12121	CARBON CATABOLITE REPRESSOR PROTEIN 4	coord: 9..152
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 66..78
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 53..88 score: 8.54379
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 17..85

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg025963.1	Spg025963.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0000175	3'-5'-exoribonuclease activity
molecular_function	GO:0005509	calcium ion binding