Spg025914 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg025914 vs. NCBI nr
Match: XP_022957184.1 (transmembrane 9 superfamily member 1-like [Cucurbita moschata] >KAG7032372.1 Transmembrane 9 superfamily member 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1015.4 bits (2624), Expect = 1.9e-292
Identity = 516/593 (87.02%), Postives = 533/593 (89.88%), Query Frame = 0

Query: 1   MSSFKRPIPITGPFI-FFFLFLLSPAFAAESDHKYQQGESVVLWVNKVGPYNNPQETYNY 60
           MSS  R IP+TGPFI FFF+F  SPA A+ESDHKYQ GESVVLWVNKVGPYNNPQETYNY
Sbjct: 1   MSSCVRRIPLTGPFIFFFFIFFRSPALASESDHKYQPGESVVLWVNKVGPYNNPQETYNY 60

Query: 61  YSLPFCHPTGDSAHKWGGLGEVLGGNELIDSRIEIKFQKNVDRTTICQLDLDEAKVKQFK 120
           YSLPFCHP+G+SAHKWGGLGEVLGGNELIDSRIE+KFQ+NVDRTTIC LDLDE  VKQFK
Sbjct: 61  YSLPFCHPSGESAHKWGGLGEVLGGNELIDSRIELKFQRNVDRTTICHLDLDETTVKQFK 120

Query: 121 DAIESNYWLEFFMDDLPLWGFVGELHSDKNSEDEKHVLYTHKNIVVKYNKDQIIHVNLTQ 180
           DAI+S+YWLEFFMDDLPLWGFVGELHSDKNSEDEKHVLYTHKNI+VKYNKDQIIHVNLTQ
Sbjct: 121 DAIKSSYWLEFFMDDLPLWGFVGELHSDKNSEDEKHVLYTHKNIIVKYNKDQIIHVNLTQ 180

Query: 181 ESPKSLDVGRSLDMTYSVKWIPTNVTFARRFDIYLDYPFFEHQ----------------- 240
           ESPKSL+VGRSLDMTYSVKWIPTNVTFARRFDIYLDYPFFEHQ                 
Sbjct: 181 ESPKSLEVGRSLDMTYSVKWIPTNVTFARRFDIYLDYPFFEHQIHWFSIFNSFMMVIFLT 240

Query: 241 ----------------------------ERDVSEESGWKLVHGDVFRSPRGLVILSAVVG 300
                                       ERDV+EESGWKLVHGDVFRSPRGLVILSAVVG
Sbjct: 241 GLVSMILMRTLRNDYAKYAREDDDLETLERDVNEESGWKLVHGDVFRSPRGLVILSAVVG 300

Query: 301 TGAQLAVLVLLVILLAIVGMLYVGRGAIITTFIVCYALTSSLSGYVSGGMYSRHGGKSWI 360
           TGAQLAVLVLLVILLAIV MLYVGRGAIITTFIVCYALTSSLSGYVSGGMYSRHGGKSWI
Sbjct: 301 TGAQLAVLVLLVILLAIVVMLYVGRGAIITTFIVCYALTSSLSGYVSGGMYSRHGGKSWI 360

Query: 361 KSMILTASLFPFTCFGIGFILNTIAIFYGSLAAIPFGTMVVVFVIWAFISFPLALLGTVV 420
           KSMILTASLFPFTCFGIGF+LN IA+FYGSLAAIPFGTMVVVFVIWAFISFPL LLGTVV
Sbjct: 361 KSMILTASLFPFTCFGIGFVLNMIAVFYGSLAAIPFGTMVVVFVIWAFISFPLVLLGTVV 420

Query: 421 GRNWSGTPNNPCRVKTIPRPIPEKKWYLTPSVVSMMGGLLPFGSIFIEMYFVFTSFWNYK 480
           GRNWSGT NNPCRVKTIPRPIPEKKWYLTPSVVS+MGGLLPFGSIFIEMYFVFTSFWNYK
Sbjct: 421 GRNWSGTANNPCRVKTIPRPIPEKKWYLTPSVVSLMGGLLPFGSIFIEMYFVFTSFWNYK 480

Query: 481 VYYVYGFMLLVFLILIIVTICVTIVGTYFLLNAENYHWQWTSFFSAASTAVYVYLYSVYY 540
           VYYVYGFMLLVFLILIIVTICVTIVGTYFLLNAENYHWQWTSFFSAASTAVYVYLYS+YY
Sbjct: 481 VYYVYGFMLLVFLILIIVTICVTIVGTYFLLNAENYHWQWTSFFSAASTAVYVYLYSIYY 540

Query: 541 YYVKTKMSGFFQTSFYFGYTLMFCLGLGILCGAVGYLGSNLFVRRIYRNIKCD 548
           YYVKTKMSGFFQTSFYFGYTLMFCLGLGILCGAVGYLGSNLFVRRIY+NIKCD
Sbjct: 541 YYVKTKMSGFFQTSFYFGYTLMFCLGLGILCGAVGYLGSNLFVRRIYKNIKCD 593

BLAST of Spg025914 vs. NCBI nr
Match: KAG6601614.1 (Transmembrane 9 superfamily member 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1015.0 bits (2623), Expect = 2.4e-292
Identity = 516/594 (86.87%), Postives = 533/594 (89.73%), Query Frame = 0

Query: 1   MSSFKRPIPITGPFI--FFFLFLLSPAFAAESDHKYQQGESVVLWVNKVGPYNNPQETYN 60
           MSS  R IP+TGPFI  FFF+F  SPA A+ESDHKYQ GESVVLWVNKVGPYNNPQETYN
Sbjct: 1   MSSCVRRIPLTGPFIIFFFFIFFRSPALASESDHKYQPGESVVLWVNKVGPYNNPQETYN 60

Query: 61  YYSLPFCHPTGDSAHKWGGLGEVLGGNELIDSRIEIKFQKNVDRTTICQLDLDEAKVKQF 120
           YYSLPFCHP+G+SAHKWGGLGEVLGGNELIDSRIE+KFQ+NVDRTTIC LDLDE  VKQF
Sbjct: 61  YYSLPFCHPSGESAHKWGGLGEVLGGNELIDSRIELKFQRNVDRTTICHLDLDETTVKQF 120

Query: 121 KDAIESNYWLEFFMDDLPLWGFVGELHSDKNSEDEKHVLYTHKNIVVKYNKDQIIHVNLT 180
           KDAI+S+YWLEFFMDDLPLWGFVGELHSDKNSEDEKHVLYTHKNI+VKYNKDQIIHVNLT
Sbjct: 121 KDAIKSSYWLEFFMDDLPLWGFVGELHSDKNSEDEKHVLYTHKNIIVKYNKDQIIHVNLT 180

Query: 181 QESPKSLDVGRSLDMTYSVKWIPTNVTFARRFDIYLDYPFFEHQ---------------- 240
           QESPKSL+VGRSLDMTYSVKWIPTNVTFARRFDIYLDYPFFEHQ                
Sbjct: 181 QESPKSLEVGRSLDMTYSVKWIPTNVTFARRFDIYLDYPFFEHQIHWFSIFNSFMMVIFL 240

Query: 241 -----------------------------ERDVSEESGWKLVHGDVFRSPRGLVILSAVV 300
                                        ERDV+EESGWKLVHGDVFRSPRGLVILSAVV
Sbjct: 241 TGLVSMILMRTLRNDYAKYAREDDDLETLERDVNEESGWKLVHGDVFRSPRGLVILSAVV 300

Query: 301 GTGAQLAVLVLLVILLAIVGMLYVGRGAIITTFIVCYALTSSLSGYVSGGMYSRHGGKSW 360
           GTGAQLAVLVLLVILLAIV MLYVGRGAIITTFIVCYALTSSLSGYVSGGMYSRHGGKSW
Sbjct: 301 GTGAQLAVLVLLVILLAIVVMLYVGRGAIITTFIVCYALTSSLSGYVSGGMYSRHGGKSW 360

Query: 361 IKSMILTASLFPFTCFGIGFILNTIAIFYGSLAAIPFGTMVVVFVIWAFISFPLALLGTV 420
           IKSMILTASLFPFTCFGIGF+LN IA+FYGSLAAIPFGTMVVVFVIWAFISFPL LLGTV
Sbjct: 361 IKSMILTASLFPFTCFGIGFVLNMIAVFYGSLAAIPFGTMVVVFVIWAFISFPLVLLGTV 420

Query: 421 VGRNWSGTPNNPCRVKTIPRPIPEKKWYLTPSVVSMMGGLLPFGSIFIEMYFVFTSFWNY 480
           VGRNWSGT NNPCRVKTIPRPIPEKKWYLTPSVVS+MGGLLPFGSIFIEMYFVFTSFWNY
Sbjct: 421 VGRNWSGTANNPCRVKTIPRPIPEKKWYLTPSVVSLMGGLLPFGSIFIEMYFVFTSFWNY 480

Query: 481 KVYYVYGFMLLVFLILIIVTICVTIVGTYFLLNAENYHWQWTSFFSAASTAVYVYLYSVY 540
           KVYYVYGFMLLVFLILIIVTICVTIVGTYFLLNAENYHWQWTSFFSAASTAVYVYLYS+Y
Sbjct: 481 KVYYVYGFMLLVFLILIIVTICVTIVGTYFLLNAENYHWQWTSFFSAASTAVYVYLYSIY 540

Query: 541 YYYVKTKMSGFFQTSFYFGYTLMFCLGLGILCGAVGYLGSNLFVRRIYRNIKCD 548
           YYYVKTKMSGFFQTSFYFGYTLMFCLGLGILCGAVGYLGSNLFVRRIY+NIKCD
Sbjct: 541 YYYVKTKMSGFFQTSFYFGYTLMFCLGLGILCGAVGYLGSNLFVRRIYKNIKCD 594

BLAST of Spg025914 vs. NCBI nr
Match: XP_022997663.1 (transmembrane 9 superfamily member 1-like [Cucurbita maxima])

HSP 1 Score: 1010.7 bits (2612), Expect = 4.6e-291
Identity = 515/596 (86.41%), Postives = 530/596 (88.93%), Query Frame = 0

Query: 1   MSSFKRPIPITGPFIFFFLF----LLSPAFAAESDHKYQQGESVVLWVNKVGPYNNPQET 60
           MSS  R IP+TGPFIFFF F     L PA A+ESDHKY  GESVVLWVNKVGPYNNPQET
Sbjct: 1   MSSSVRRIPLTGPFIFFFFFFFLSFLPPALASESDHKYHPGESVVLWVNKVGPYNNPQET 60

Query: 61  YNYYSLPFCHPTGDSAHKWGGLGEVLGGNELIDSRIEIKFQKNVDRTTICQLDLDEAKVK 120
           YNYYSLPFCHP+G+SAHKWGGLGEVLGGNELIDSRIE+KFQ+NVDRTTIC LDLDE  VK
Sbjct: 61  YNYYSLPFCHPSGESAHKWGGLGEVLGGNELIDSRIELKFQRNVDRTTICHLDLDETTVK 120

Query: 121 QFKDAIESNYWLEFFMDDLPLWGFVGELHSDKNSEDEKHVLYTHKNIVVKYNKDQIIHVN 180
           QFKDAI+S+YWLEFFMDDLPLWGFVGELHSDKNSEDEKHVLYTHKNI+VKYNKDQIIHVN
Sbjct: 121 QFKDAIKSSYWLEFFMDDLPLWGFVGELHSDKNSEDEKHVLYTHKNIIVKYNKDQIIHVN 180

Query: 181 LTQESPKSLDVGRSLDMTYSVKWIPTNVTFARRFDIYLDYPFFEHQ-------------- 240
           LTQESPKSL+VGRSLDMTYSVKWIPTNVTF RRFDIYLDYPFFEHQ              
Sbjct: 181 LTQESPKSLEVGRSLDMTYSVKWIPTNVTFTRRFDIYLDYPFFEHQIHWFSIFNSFMMVI 240

Query: 241 -------------------------------ERDVSEESGWKLVHGDVFRSPRGLVILSA 300
                                          ERDV+EESGWKLVHGDVFRSPRGLVILSA
Sbjct: 241 FLTGLVSMILMRTLRNDYAKYAREDDDLETLERDVNEESGWKLVHGDVFRSPRGLVILSA 300

Query: 301 VVGTGAQLAVLVLLVILLAIVGMLYVGRGAIITTFIVCYALTSSLSGYVSGGMYSRHGGK 360
           VVGTGAQLAVLVLLVILLAIV MLYVGRGAIITTFIVCYALTSSLSGYVSGGMYSRHGGK
Sbjct: 301 VVGTGAQLAVLVLLVILLAIVVMLYVGRGAIITTFIVCYALTSSLSGYVSGGMYSRHGGK 360

Query: 361 SWIKSMILTASLFPFTCFGIGFILNTIAIFYGSLAAIPFGTMVVVFVIWAFISFPLALLG 420
           SWIKSMILTASLFPFTCFGIGF+LN IAIFYGSLAAIPFGTMVVVFVIWAFISFPL LLG
Sbjct: 361 SWIKSMILTASLFPFTCFGIGFVLNMIAIFYGSLAAIPFGTMVVVFVIWAFISFPLVLLG 420

Query: 421 TVVGRNWSGTPNNPCRVKTIPRPIPEKKWYLTPSVVSMMGGLLPFGSIFIEMYFVFTSFW 480
           TVVGRNWSGT NNPCRVKTIPRPIPEKKWYLTPSVVS+MGGLLPFGSIFIEMYFVFTSFW
Sbjct: 421 TVVGRNWSGTANNPCRVKTIPRPIPEKKWYLTPSVVSLMGGLLPFGSIFIEMYFVFTSFW 480

Query: 481 NYKVYYVYGFMLLVFLILIIVTICVTIVGTYFLLNAENYHWQWTSFFSAASTAVYVYLYS 540
           NYKVYYVYGFMLLVFLILIIVTICVTIVGTYFLLNAENYHWQWTSFFSAASTAVYVYLYS
Sbjct: 481 NYKVYYVYGFMLLVFLILIIVTICVTIVGTYFLLNAENYHWQWTSFFSAASTAVYVYLYS 540

Query: 541 VYYYYVKTKMSGFFQTSFYFGYTLMFCLGLGILCGAVGYLGSNLFVRRIYRNIKCD 548
           +YYYYVKTKMSGFFQTSFYFGYTLMFCLGLGILCGAVGYLGSNLFVRRIY+NIKCD
Sbjct: 541 IYYYYVKTKMSGFFQTSFYFGYTLMFCLGLGILCGAVGYLGSNLFVRRIYKNIKCD 596

BLAST of Spg025914 vs. NCBI nr
Match: XP_023528707.1 (transmembrane 9 superfamily member 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1010.0 bits (2610), Expect = 7.9e-291
Identity = 515/595 (86.55%), Postives = 532/595 (89.41%), Query Frame = 0

Query: 1   MSSFKRPIPITGPFI---FFFLFLLSPAFAAESDHKYQQGESVVLWVNKVGPYNNPQETY 60
           MSS  R IP+TGPFI   FFFLF LS A A+ESDHKYQ GESVVLWVNKVGPYNNPQETY
Sbjct: 1   MSSCVRRIPLTGPFIFFFFFFLFFLSSALASESDHKYQPGESVVLWVNKVGPYNNPQETY 60

Query: 61  NYYSLPFCHPTGDSAHKWGGLGEVLGGNELIDSRIEIKFQKNVDRTTICQLDLDEAKVKQ 120
           NYYSLPFCHP+G+SAHKWGGLGEVLGGNELIDSRIE+KFQ+NVDRTTIC LDLDE  VKQ
Sbjct: 61  NYYSLPFCHPSGESAHKWGGLGEVLGGNELIDSRIELKFQRNVDRTTICHLDLDEITVKQ 120

Query: 121 FKDAIESNYWLEFFMDDLPLWGFVGELHSDKNSEDEKHVLYTHKNIVVKYNKDQIIHVNL 180
           FKDAI+S+YWLEFFMDDLPLWGFVGELHSDKNS+ EKHVLYTHKNI+VKYNKDQIIHVNL
Sbjct: 121 FKDAIKSSYWLEFFMDDLPLWGFVGELHSDKNSDAEKHVLYTHKNIIVKYNKDQIIHVNL 180

Query: 181 TQESPKSLDVGRSLDMTYSVKWIPTNVTFARRFDIYLDYPFFEHQ--------------- 240
           TQESPKSL+VGRSLDMTYSVKWIPTNVTFARRFDIYLDYPFFEHQ               
Sbjct: 181 TQESPKSLEVGRSLDMTYSVKWIPTNVTFARRFDIYLDYPFFEHQIHWFSIFNSFMMVIF 240

Query: 241 ------------------------------ERDVSEESGWKLVHGDVFRSPRGLVILSAV 300
                                         ERDV+EESGWKLVHGDVFRSPRGLVILSAV
Sbjct: 241 LTGLVSMILMRTLRNDYAKYAREDDDLETLERDVNEESGWKLVHGDVFRSPRGLVILSAV 300

Query: 301 VGTGAQLAVLVLLVILLAIVGMLYVGRGAIITTFIVCYALTSSLSGYVSGGMYSRHGGKS 360
           VGTGAQLAVLVLLVILLAIV MLYVGRGAIITTFIVCYALTSSLSGYVSGGMYSRHGGKS
Sbjct: 301 VGTGAQLAVLVLLVILLAIVVMLYVGRGAIITTFIVCYALTSSLSGYVSGGMYSRHGGKS 360

Query: 361 WIKSMILTASLFPFTCFGIGFILNTIAIFYGSLAAIPFGTMVVVFVIWAFISFPLALLGT 420
           WIKSMILTASLFPFTCFGIGF+LN IA+FYGSLAAIPFGTMVVVFVIWAFISFPL LLGT
Sbjct: 361 WIKSMILTASLFPFTCFGIGFVLNMIAVFYGSLAAIPFGTMVVVFVIWAFISFPLVLLGT 420

Query: 421 VVGRNWSGTPNNPCRVKTIPRPIPEKKWYLTPSVVSMMGGLLPFGSIFIEMYFVFTSFWN 480
           VVGRNWSGT NNPCRVKTIPRPIPEKKWYLTPSVVS+MGGLLPFGSIFIEMYFVFTSFWN
Sbjct: 421 VVGRNWSGTANNPCRVKTIPRPIPEKKWYLTPSVVSLMGGLLPFGSIFIEMYFVFTSFWN 480

Query: 481 YKVYYVYGFMLLVFLILIIVTICVTIVGTYFLLNAENYHWQWTSFFSAASTAVYVYLYSV 540
           YKVYYVYGFMLLVFLILIIVTICVTIVGTYFLLNAENYHWQWTSFFSAASTAVYVYLYS+
Sbjct: 481 YKVYYVYGFMLLVFLILIIVTICVTIVGTYFLLNAENYHWQWTSFFSAASTAVYVYLYSI 540

Query: 541 YYYYVKTKMSGFFQTSFYFGYTLMFCLGLGILCGAVGYLGSNLFVRRIYRNIKCD 548
           YYYYVKTKMSGFFQTSFYFGYTLMFCLGLGILCGAVGYLGSNLFVRRIY+NIKCD
Sbjct: 541 YYYYVKTKMSGFFQTSFYFGYTLMFCLGLGILCGAVGYLGSNLFVRRIYKNIKCD 595

BLAST of Spg025914 vs. NCBI nr
Match: XP_022945976.1 (transmembrane 9 superfamily member 1-like [Cucurbita moschata])

HSP 1 Score: 1001.5 bits (2588), Expect = 2.8e-288
Identity = 511/596 (85.74%), Postives = 531/596 (89.09%), Query Frame = 0

Query: 1   MSSFKRPIPITGPFI---FFFLFLLSPAFAAESDHKYQQGESVVLWVNKVGPYNNPQETY 60
           MSSF R IP+T PFI   FFFLFLLSPA AAESDHKYQ GESVVLWVNKVGPYNNPQETY
Sbjct: 1   MSSFVRFIPLTWPFIIIFFFFLFLLSPALAAESDHKYQPGESVVLWVNKVGPYNNPQETY 60

Query: 61  NYYSLPFCHPTGDSAHKWGGLGEVLGGNELIDSRIEIKFQKNVDRTTICQLDLDEAKVKQ 120
           NYYSLPFCHP+GDSAHKWGGLGEVLGGNELIDSRIEIKF KN+DRTTIC L LDEAKVK 
Sbjct: 61  NYYSLPFCHPSGDSAHKWGGLGEVLGGNELIDSRIEIKFLKNMDRTTICPLHLDEAKVKL 120

Query: 121 FKDAIESNYWLEFFMDDLPLWGFVGELHSDKNSEDEKHVLYTHKNIVVKYNKDQIIHVNL 180
           FK+AI+ +YWLE F+DDLPLWGFVGEL SDKNSEDEKH+LYTHKNI++KYNKDQIIHVNL
Sbjct: 121 FKNAIQRSYWLELFIDDLPLWGFVGELPSDKNSEDEKHILYTHKNIIIKYNKDQIIHVNL 180

Query: 181 TQESPKSLDVGRSLDMTYSVKWIPTNVTFARRFDIYLDYPFFEHQ--------------- 240
           TQESPKSL+VGRSLDMTYSVKWIPTNVTFARRFDIYLDYPFFEHQ               
Sbjct: 181 TQESPKSLEVGRSLDMTYSVKWIPTNVTFARRFDIYLDYPFFEHQIHWFSIFNSFMMVIF 240

Query: 241 -------------------------------ERDVSEESGWKLVHGDVFRSPRGLVILSA 300
                                          ERDVSEESGWKLVHGDVFRSPR LVILSA
Sbjct: 241 LTGLVSMILMRTLRNDYAKYAREDDDDLETLERDVSEESGWKLVHGDVFRSPRCLVILSA 300

Query: 301 VVGTGAQLAVLVLLVILLAIVGMLYVGRGAIITTFIVCYALTSSLSGYVSGGMYSRHGGK 360
           VVGTGAQLAVLVLLVILLAI+GMLYVGRGAIITTFIVCYALTSSLSGYVSGGMYSRHGGK
Sbjct: 301 VVGTGAQLAVLVLLVILLAIIGMLYVGRGAIITTFIVCYALTSSLSGYVSGGMYSRHGGK 360

Query: 361 SWIKSMILTASLFPFTCFGIGFILNTIAIFYGSLAAIPFGTMVVVFVIWAFISFPLALLG 420
           SWIKSMILTASLFPF CFGIG +LNTIAIFYGSLAAIPFGTMVVVFVIWAFI FPLALLG
Sbjct: 361 SWIKSMILTASLFPFMCFGIGLVLNTIAIFYGSLAAIPFGTMVVVFVIWAFIYFPLALLG 420

Query: 421 TVVGRNWSGTPNNPCRVKTIPRPIPEKKWYLTPSVVSMMGGLLPFGSIFIEMYFVFTSFW 480
           TVVGRNWSGTPNNPCRVKTIPRP+PEKKWYLTPSVVS+MGGLLPFGSIFIEMYFVFTSFW
Sbjct: 421 TVVGRNWSGTPNNPCRVKTIPRPVPEKKWYLTPSVVSLMGGLLPFGSIFIEMYFVFTSFW 480

Query: 481 NYKVYYVYGFMLLVFLILIIVTICVTIVGTYFLLNAENYHWQWTSFFSAASTAVYVYLYS 540
           NYKVYYVYGFMLLVFLILIIVTICVTIVGTYFLLNAENYHWQWTSFFSAASTAVY++LYS
Sbjct: 481 NYKVYYVYGFMLLVFLILIIVTICVTIVGTYFLLNAENYHWQWTSFFSAASTAVYLFLYS 540

Query: 541 VYYYYVKTKMSGFFQTSFYFGYTLMFCLGLGILCGAVGYLGSNLFVRRIYRNIKCD 548
           +YY+YVKT+MSGFFQTSFYFGYTLMFCLGLGILCGAVGYLGSNLFVRRIYRNIKCD
Sbjct: 541 IYYFYVKTRMSGFFQTSFYFGYTLMFCLGLGILCGAVGYLGSNLFVRRIYRNIKCD 596

BLAST of Spg025914 vs. ExPASy Swiss-Prot
Match: Q940G0 (Transmembrane 9 superfamily member 1 OS=Arabidopsis thaliana OX=3702 GN=TMN1 PE=1 SV=1)

HSP 1 Score: 929.9 bits (2402), Expect = 1.4e-269
Identity = 463/572 (80.94%), Postives = 496/572 (86.71%), Query Frame = 0

Query: 21  LLSPAFAAESDHKYQQGESVVLWVNKVGPYNNPQETYNYYSLPFCHPTGDSAHKWGGLGE 80
           LL+P FA++SDHKYQ  E V LWVNKVGPYNNPQETYNYYSLPFC P+G++ HKWGGLGE
Sbjct: 18  LLTPTFASDSDHKYQAEEQVTLWVNKVGPYNNPQETYNYYSLPFCRPSGNNVHKWGGLGE 77

Query: 81  VLGGNELIDSRIEIKFQKNVDRTTICQLDLDEAKVKQFKDAIESNYWLEFFMDDLPLWGF 140
           VLGGNELIDS I IKF KNV+R+ IC L+LDEAKVK FKDAIES+YW EFFMDDLPLWGF
Sbjct: 78  VLGGNELIDSEIAIKFMKNVERSVICPLELDEAKVKHFKDAIESSYWFEFFMDDLPLWGF 137

Query: 141 VGELHSDKNSEDEKHVLYTHKNIVVKYNKDQIIHVNLTQESPKSLDVGRSLDMTYSVKWI 200
           VGELH DKNSE+ KHVLYTHKNIVVKYNKDQIIHVNLTQ++P+ L+ G+ +D+TYSV+WI
Sbjct: 138 VGELHPDKNSENGKHVLYTHKNIVVKYNKDQIIHVNLTQDNPRPLEAGKKMDLTYSVQWI 197

Query: 201 PTNVTFARRFDIYLDYPFFEHQ-------------------------------------- 260
           PTNVTFARRFD+YLDYPFFEHQ                                      
Sbjct: 198 PTNVTFARRFDVYLDYPFFEHQIHWFSIFNSFMMVIFLTGLVSMILMRTLRNDYAKYARE 257

Query: 261 -------ERDVSEESGWKLVHGDVFRSPRGLVILSAVVGTGAQLAVLVLLVILLAIVGML 320
                  ERDVSEESGWKLVHGDVFR    LV+LSAVVGTGAQLA+LVLLVIL+AIVG L
Sbjct: 258 DDDLESLERDVSEESGWKLVHGDVFRPASSLVLLSAVVGTGAQLALLVLLVILMAIVGTL 317

Query: 321 YVGRGAIITTFIVCYALTSSLSGYVSGGMYSRHGGKSWIKSMILTASLFPFTCFGIGFIL 380
           YVGRGAI+TTFIVCYALTS +SGYVSGGMYSR GGK WIK M+LTASLFPF CFGIGF+L
Sbjct: 318 YVGRGAIVTTFIVCYALTSFVSGYVSGGMYSRSGGKHWIKCMVLTASLFPFLCFGIGFLL 377

Query: 381 NTIAIFYGSLAAIPFGTMVVVFVIWAFISFPLALLGTVVGRNWSGTPNNPCRVKTIPRPI 440
           NTIAIFYGSLAAIPFGTMVVVFVIW FISFPLALLGTVVGRNWSG PNNPCRVKTIPRPI
Sbjct: 378 NTIAIFYGSLAAIPFGTMVVVFVIWGFISFPLALLGTVVGRNWSGAPNNPCRVKTIPRPI 437

Query: 441 PEKKWYLTPSVVSMMGGLLPFGSIFIEMYFVFTSFWNYKVYYVYGFMLLVFLILIIVTIC 500
           PEKKWYLTPSVVS+MGGLLPFGSIFIEMYFVFTSFWNYKVYYVYGFMLLVF+IL+IVT+C
Sbjct: 438 PEKKWYLTPSVVSLMGGLLPFGSIFIEMYFVFTSFWNYKVYYVYGFMLLVFVILVIVTVC 497

Query: 501 VTIVGTYFLLNAENYHWQWTSFFSAASTAVYVYLYSVYYYYVKTKMSGFFQTSFYFGYTL 548
           VTIVGTYFLLNAENYHWQWTSFFSAASTAVYVYLYS+YYYYVKTKMSGFFQTSFYFGYT+
Sbjct: 498 VTIVGTYFLLNAENYHWQWTSFFSAASTAVYVYLYSIYYYYVKTKMSGFFQTSFYFGYTM 557

BLAST of Spg025914 vs. ExPASy Swiss-Prot
Match: Q9HD45 (Transmembrane 9 superfamily member 3 OS=Homo sapiens OX=9606 GN=TM9SF3 PE=1 SV=2)

HSP 1 Score: 542.0 bits (1395), Expect = 8.0e-153
Identity = 290/592 (48.99%), Postives = 386/592 (65.20%), Query Frame = 0

Query: 6   RPIP-----ITGPFIFFFLFLLSPAFAAESDHKYQQGESVVLWVNKVGPYNNPQETYNYY 65
           RP+P          ++  L LL    A E +H YQ  E VVLW+N VGPY+N QETY Y+
Sbjct: 2   RPLPGALGVAAAAALWLLLLLLPRTRADEHEHTYQDKEEVVLWMNTVGPYHNRQETYKYF 61

Query: 66  SLPFCHPTGDS-AHKWGGLGEVLGGNELIDSRIEIKFQKNVDRTTICQLDLDEAKVKQFK 125
           SLPFC  +  S +H    LGE L G EL  S ++IKF+ +V   T C++DLD+ K   F 
Sbjct: 62  SLPFCVGSKKSISHYHETLGEALQGVELEFSGLDIKFKDDVMPATYCEIDLDKEKRDAFV 121

Query: 126 DAIESNYWLEFFMDDLPLWGFVGELHSDKNSEDEKHVLYTHKNIVVKYNKDQIIHVNLTQ 185
            AI+++YW + ++DDLP+WG VGE  +D+N ED  + L+T+K + + +N ++I+ VNLT 
Sbjct: 122 YAIKNHYWYQMYIDDLPIWGIVGE--ADENGED--YYLWTYKKLEIGFNGNRIVDVNLTS 181

Query: 186 ESPKSLDVGRSLDMTYSVKWIPTNVTFARRFDIYLDYPFFEHQ----------------- 245
           E    L     + M+YSVKW  ++V F  RFD YLD  FF+H+                 
Sbjct: 182 EGKVKLVPNTKIQMSYSVKWKKSDVKFEDRFDKYLDPSFFQHRIHWFSIFNSFMMVIFLV 241

Query: 246 ---------------------------ERDVSEESGWKLVHGDVFRSPRGLVILSAVVGT 305
                                      +RD+ +E GWK VHGDVFR     +I S+++G+
Sbjct: 242 GLVSMILMRTLRKDYARYSKEEEMDDMDRDLGDEYGWKQVHGDVFRPSSHPLIFSSLIGS 301

Query: 306 GAQLAVLVLLVILLAIVGMLYVGRGAIITTFIVCYALTSSLSGYVSGGMYSRHGGKSWIK 365
           G Q+  + L+VI++A++  LY  RG++++T I  YA TS ++GY  G +Y+R GG+ WIK
Sbjct: 302 GCQIFAVSLIVIIVAMIEDLYTERGSMLSTAIFVYAATSPVNGYFGGSLYARQGGRRWIK 361

Query: 366 SMILTASLFPFTCFGIGFILNTIAIFYGSLAAIPFGTMVVVFVIWAFISFPLALLGTVVG 425
            M + A L P    G  F +N IAI+Y +  AIPFGTMV V  I  F+  PL L+GT++G
Sbjct: 362 QMFIGAFLIPAMVCGTAFFINFIAIYYHASRAIPFGTMVAVCCICFFVILPLNLVGTILG 421

Query: 426 RNWSGTPNNPCRVKTIPRPIPEKKWYLTPSVVSMMGGLLPFGSIFIEMYFVFTSFWNYKV 485
           RN SG PN PCRV  +PRPIPEKKW++ P+V+  +GG+LPFGSIFIEMYF+FTSFW YK+
Sbjct: 422 RNLSGQPNFPCRVNAVPRPIPEKKWFMEPAVIVCLGGILPFGSIFIEMYFIFTSFWAYKI 481

Query: 486 YYVYGFMLLVFLILIIVTICVTIVGTYFLLNAENYHWQWTSFFSAASTAVYVYLYSVYYY 545
           YYVYGFM+LV +IL IVT+CVTIV TYFLLNAE+Y WQWTSF SAASTA+YVY+YS YYY
Sbjct: 482 YYVYGFMMLVLVILCIVTVCVTIVCTYFLLNAEDYRWQWTSFLSAASTAIYVYMYSFYYY 541

Query: 546 YVKTKMSGFFQTSFYFGYTLMFCLGLGILCGAVGYLGSNLFVRRIYRNIKCD 548
           + KTKM G FQTSFYFGY  +F   LGI+CGA+GY+G++ FVR+IY N+K D
Sbjct: 542 FFKTKMYGLFQTSFYFGYMAVFSTALGIMCGAIGYMGTSAFVRKIYTNVKID 589

BLAST of Spg025914 vs. ExPASy Swiss-Prot
Match: Q9ET30 (Transmembrane 9 superfamily member 3 OS=Mus musculus OX=10090 GN=Tm9sf3 PE=1 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 1.4e-152
Identity = 287/574 (50.00%), Postives = 381/574 (66.38%), Query Frame = 0

Query: 19  LFLLSPAFAAESDHKYQQGESVVLWVNKVGPYNNPQETYNYYSLPFCHPTGDS-AHKWGG 78
           L LL  A + E +H YQ  E VVLW+N VGPY+N QETY Y+SLPFC  +  S +H    
Sbjct: 18  LLLLPRARSDEHEHTYQDKEEVVLWMNTVGPYHNRQETYKYFSLPFCVGSKKSISHYHET 77

Query: 79  LGEVLGGNELIDSRIEIKFQKNVDRTTICQLDLDEAKVKQFKDAIESNYWLEFFMDDLPL 138
           LGE L G EL  S ++IKF+ +V   T C++DLD+ K   F  AI+++YW + ++DDLP+
Sbjct: 78  LGEALQGVELEFSGLDIKFKDDVMPGTYCEIDLDKEKRDAFVYAIKNHYWYQMYIDDLPI 137

Query: 139 WGFVGELHSDKNSEDEKHVLYTHKNIVVKYNKDQIIHVNLTQESPKSLDVGRSLDMTYSV 198
           WG VGE  +D+N ED  + L+T+K + + +N ++I+ VNLT E    L     + M+YSV
Sbjct: 138 WGIVGE--ADENGED--YYLWTYKKLEIGFNGNRIVDVNLTSEGKVKLVPNTKIQMSYSV 197

Query: 199 KWIPTNVTFARRFDIYLDYPFFEHQ----------------------------------- 258
           KW  ++V F  RFD YLD  FF+H+                                   
Sbjct: 198 KWKKSDVKFEDRFDKYLDPSFFQHRIHWFSIFNSFMMVIFLVGLVSMILMRTLRKDYARY 257

Query: 259 ---------ERDVSEESGWKLVHGDVFRSPRGLVILSAVVGTGAQLAVLVLLVILLAIVG 318
                    +RD+ +E GWK VHGDVFR     +I S+++G+G Q+  + L+VI++A++ 
Sbjct: 258 SKEEEMDDMDRDLGDEYGWKQVHGDVFRPSSHPLIFSSLIGSGCQIFAVSLIVIIVAMIE 317

Query: 319 MLYVGRGAIITTFIVCYALTSSLSGYVSGGMYSRHGGKSWIKSMILTASLFPFTCFGIGF 378
            LY  RG++++T I  YA TS ++GY  G +Y+R GG+ WIK M + A L P    G  F
Sbjct: 318 DLYTERGSMLSTAIFVYAATSPVNGYFGGSLYARQGGRRWIKQMFIGAFLIPAMVCGTAF 377

Query: 379 ILNTIAIFYGSLAAIPFGTMVVVFVIWAFISFPLALLGTVVGRNWSGTPNNPCRVKTIPR 438
            +N IAI+Y +  AIPFGTMV V  I  F+  PL L+GT++GRN SG PN PCRV  +PR
Sbjct: 378 FINFIAIYYHASRAIPFGTMVAVCCICFFVILPLNLVGTILGRNLSGQPNFPCRVNAVPR 437

Query: 439 PIPEKKWYLTPSVVSMMGGLLPFGSIFIEMYFVFTSFWNYKVYYVYGFMLLVFLILIIVT 498
           PIPEKKW++ P+V+  +GG+LPFGSIFIEMYF+FTSFW YK+YYVYGFM+LV +IL IVT
Sbjct: 438 PIPEKKWFMEPAVIVCLGGILPFGSIFIEMYFIFTSFWAYKIYYVYGFMMLVLVILCIVT 497

Query: 499 ICVTIVGTYFLLNAENYHWQWTSFFSAASTAVYVYLYSVYYYYVKTKMSGFFQTSFYFGY 548
           +CVTIV TYFLLNAE+Y WQWTSF SAASTA+YVY+YS YYY+ KTKM G FQTSFYFGY
Sbjct: 498 VCVTIVCTYFLLNAEDYRWQWTSFLSAASTAIYVYMYSFYYYFFKTKMYGLFQTSFYFGY 557

BLAST of Spg025914 vs. ExPASy Swiss-Prot
Match: Q9FHT4 (Transmembrane 9 superfamily member 4 OS=Arabidopsis thaliana OX=3702 GN=TMN4 PE=2 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 8.0e-113
Identity = 218/582 (37.46%), Postives = 334/582 (57.39%), Query Frame = 0

Query: 15  IFFFLFLLSPAFAAESDHKYQQGESVVLWVNKVGPYNNPQETYNYYSLPFCHPTGDSAHK 74
           +F FL+ +SP  +  SDH+Y+ G+ V L+ NKVGP++NP ETY Y+ LPFC  +     K
Sbjct: 13  VFLFLYGVSPVISDGSDHRYKVGDDVPLYANKVGPFHNPSETYRYFDLPFC-SSAPVKEK 72

Query: 75  WGGLGEVLGGNELIDSRIEIKFQKNVDRTTICQLDLDEAKVKQFKDAIESNYWLEFFMDD 134
              LGEVL G+ L+ +  +++F    +    C+  L    V +F+D I  +Y+ + + DD
Sbjct: 73  KEALGEVLNGDRLVSAPYKLEFLGEKNSEVACRKRLSREDVAKFRDVIAKDYYFQMYYDD 132

Query: 135 LPLWGFVGELHSDKNSE--DEKHVLYTHKNIVVKYNKDQIIHVNLTQESPKSLDVGR--- 194
           LP+WGF+G++  +  ++  + K+ L+ H    + YNKD++I + +  +    +D+     
Sbjct: 133 LPIWGFLGKVVKEGKTDPSEYKYYLFNHLQFEIFYNKDRVIEIIVRTDQNFLVDLTEDKE 192

Query: 195 -SLDMTYSVKWIPTNVTFARRFDIYL---------------------------------- 254
             +D TY+V+W  T + F +R + Y                                   
Sbjct: 193 VQVDFTYTVRWKETEIPFEKRMEKYSLASSMPHHLEIHWFSIINSCVTVLLLTGFLATIL 252

Query: 255 ------DYPFFEHQER--DVSEESGWKLVHGDVFRSPRGLVILSAVVGTGAQLAVLVLLV 314
                 D+  + H E   D  EE+GWKL+HGDVFR P+   +L+A +G+G QL  L + +
Sbjct: 253 MRVLKNDFVKYAHDEEAVDDQEETGWKLIHGDVFRFPKHKSLLAAALGSGTQLFTLAVFI 312

Query: 315 ILLAIVGMLY-VGRGAIITTFIVCYALTSSLSGYVSGGMYSRHGGKSWIKSMILTASLFP 374
            +LA+VG+ Y   RGA+ T  +V YALTS ++GY +   Y +  G +W++++ILT SLF 
Sbjct: 313 FMLALVGVFYPYNRGALFTALVVIYALTSGIAGYTAASFYCQLEGTNWVRNVILTGSLFC 372

Query: 375 FTCFGIGFILNTIAIFYGSLAAIPFGTMVVVFVIWAFISFPLALLGTVVGRNWSGTPNNP 434
                    LNT+AI Y + AA+PFGT+VV+F+IWA ++ PL +LG + G+N       P
Sbjct: 373 GPLLITFSFLNTVAIAYQATAALPFGTIVVIFLIWALVTSPLLILGGIAGKNRKSEFQAP 432

Query: 435 CRVKTIPRPIPEKKWYLTPSVVSMMGGLLPFGSIFIEMYFVFTSFWNYKVYYVYGFMLLV 494
           CR    PR IP  +WY        M G LPF +I+IE+Y++F S W +++Y +Y  + +V
Sbjct: 433 CRTTKYPREIPPMRWYRRTLPQMAMAGFLPFSAIYIELYYIFASVWGHRIYTIYSILSIV 492

Query: 495 FLILIIVTICVTIVGTYFLLNAENYHWQWTSFFSAASTAVYVYLYSVYYYYVKTKMSGFF 548
           FLIL+IVT  +T+  TYF L AE++ W W S     ST +++Y Y +YYYY ++ MSGF 
Sbjct: 493 FLILVIVTAFITVALTYFQLAAEDHEWWWRSLLCGGSTGLFIYAYCLYYYYARSDMSGFM 552

BLAST of Spg025914 vs. ExPASy Swiss-Prot
Match: Q940S0 (Transmembrane 9 superfamily member 2 OS=Arabidopsis thaliana OX=3702 GN=TMN2 PE=2 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 1.4e-112
Identity = 217/567 (38.27%), Postives = 326/567 (57.50%), Query Frame = 0

Query: 30  SDHKYQQGESVVLWVNKVGPYNNPQETYNYYSLPFCHPTGDSAHKWGGLGEVLGGNELID 89
           SDH+Y++G++V L+ NKVGP++NP ETY Y+ LPFC P G    K   LGEVL G+ L+ 
Sbjct: 27  SDHRYKEGDTVPLYANKVGPFHNPSETYRYFDLPFCIPEGVKEKK-EALGEVLNGDRLVS 86

Query: 90  SRIEIKFQKNVDRTTICQLDLDEAKVKQFKDAIESNYWLEFFMDDLPLWGFVGELHSDKN 149
           +  ++ F+   +    C   L + +VKQF+ A+E +Y+ + + DDLP+WGF+G++  D  
Sbjct: 87  APYKLNFRDEKESEVYCNKKLSKEEVKQFRKAVEKDYYFQMYYDDLPIWGFIGKVDKDIK 146

Query: 150 SE--DEKHVLYTHKNIVVKYNKDQIIHVNLTQESPKSLDVGR----SLDMTYSVKWIPTN 209
           S+  + K+ LY H    + YNKD++I ++   +    +D+        +  Y+VKW  T 
Sbjct: 147 SDPSEFKYFLYKHIQFEILYNKDRVIEISARMDPHSLVDLTEDKEVDAEFMYTVKWKETE 206

Query: 210 VTFARRFDIY---------LDYPFF---------------------------------EH 269
             F +R + Y         L+  +F                                 + 
Sbjct: 207 TPFEKRMEKYSMSSSLPHHLEIHWFSIINSCVTVLLLTGFLATILMRVLKNDFMKYAQDE 266

Query: 270 QERDVSEESGWKLVHGDVFRSPRGLVILSAVVGTGAQLAVLVLLVILLAIVGMLY-VGRG 329
           +  D  EE+GWK +HGDVFR P    + +A +G+G QL  L + + +LA+VG+ Y   RG
Sbjct: 267 EAADDQEETGWKYIHGDVFRFPTHNSLFAASLGSGTQLFTLTIFIFMLALVGVFYPYNRG 326

Query: 330 AIITTFIVCYALTSSLSGYVSGGMYSRHGGKSWIKSMILTASLFPFTCFGIGFILNTIAI 389
           A+ T  +V YALTS ++GY S   Y +  GKSW+++++LT  LF    F     LNT+AI
Sbjct: 327 ALFTALVVIYALTSGIAGYTSASFYCQLEGKSWVRNLLLTGCLFCGPLFLTFCFLNTVAI 386

Query: 390 FYGSLAAIPFGTMVVVFVIWAFISFPLALLGTVVGRNWSGTPNNPCRVKTIPRPIPEKKW 449
            Y + AA+PFGT+VV+ +IW  ++ PL +LG + G+N       PCR    PR IP   W
Sbjct: 387 TYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGKNSKAEFQAPCRTTKYPREIPPLPW 446

Query: 450 YLTPSVVSMMGGLLPFGSIFIEMYFVFTSFWNYKVYYVYGFMLLVFLILIIVTICVTIVG 509
           Y +      M G LPF +I+IE+Y++F S W +++Y +Y  + +VF+ILIIVT  +T+  
Sbjct: 447 YRSAIPQMAMAGFLPFSAIYIELYYIFASVWGHRIYTIYSILFIVFIILIIVTAFITVAL 506

Query: 510 TYFLLNAENYHWQWTSFFSAASTAVYVYLYSVYYYYVKTKMSGFFQTSFYFGYTLMFCLG 548
           TYF L AE++ W W SF    ST +++Y Y +YYYY ++ MSGF QTSF+FGY    C G
Sbjct: 507 TYFQLAAEDHQWWWRSFLCGGSTGLFIYAYCLYYYYARSDMSGFMQTSFFFGYMACICYG 566

BLAST of Spg025914 vs. ExPASy TrEMBL
Match: A0A6J1H181 (Transmembrane 9 superfamily member OS=Cucurbita moschata OX=3662 GN=LOC111458653 PE=3 SV=1)

HSP 1 Score: 1015.4 bits (2624), Expect = 9.1e-293
Identity = 516/593 (87.02%), Postives = 533/593 (89.88%), Query Frame = 0

Query: 1   MSSFKRPIPITGPFI-FFFLFLLSPAFAAESDHKYQQGESVVLWVNKVGPYNNPQETYNY 60
           MSS  R IP+TGPFI FFF+F  SPA A+ESDHKYQ GESVVLWVNKVGPYNNPQETYNY
Sbjct: 1   MSSCVRRIPLTGPFIFFFFIFFRSPALASESDHKYQPGESVVLWVNKVGPYNNPQETYNY 60

Query: 61  YSLPFCHPTGDSAHKWGGLGEVLGGNELIDSRIEIKFQKNVDRTTICQLDLDEAKVKQFK 120
           YSLPFCHP+G+SAHKWGGLGEVLGGNELIDSRIE+KFQ+NVDRTTIC LDLDE  VKQFK
Sbjct: 61  YSLPFCHPSGESAHKWGGLGEVLGGNELIDSRIELKFQRNVDRTTICHLDLDETTVKQFK 120

Query: 121 DAIESNYWLEFFMDDLPLWGFVGELHSDKNSEDEKHVLYTHKNIVVKYNKDQIIHVNLTQ 180
           DAI+S+YWLEFFMDDLPLWGFVGELHSDKNSEDEKHVLYTHKNI+VKYNKDQIIHVNLTQ
Sbjct: 121 DAIKSSYWLEFFMDDLPLWGFVGELHSDKNSEDEKHVLYTHKNIIVKYNKDQIIHVNLTQ 180

Query: 181 ESPKSLDVGRSLDMTYSVKWIPTNVTFARRFDIYLDYPFFEHQ----------------- 240
           ESPKSL+VGRSLDMTYSVKWIPTNVTFARRFDIYLDYPFFEHQ                 
Sbjct: 181 ESPKSLEVGRSLDMTYSVKWIPTNVTFARRFDIYLDYPFFEHQIHWFSIFNSFMMVIFLT 240

Query: 241 ----------------------------ERDVSEESGWKLVHGDVFRSPRGLVILSAVVG 300
                                       ERDV+EESGWKLVHGDVFRSPRGLVILSAVVG
Sbjct: 241 GLVSMILMRTLRNDYAKYAREDDDLETLERDVNEESGWKLVHGDVFRSPRGLVILSAVVG 300

Query: 301 TGAQLAVLVLLVILLAIVGMLYVGRGAIITTFIVCYALTSSLSGYVSGGMYSRHGGKSWI 360
           TGAQLAVLVLLVILLAIV MLYVGRGAIITTFIVCYALTSSLSGYVSGGMYSRHGGKSWI
Sbjct: 301 TGAQLAVLVLLVILLAIVVMLYVGRGAIITTFIVCYALTSSLSGYVSGGMYSRHGGKSWI 360

Query: 361 KSMILTASLFPFTCFGIGFILNTIAIFYGSLAAIPFGTMVVVFVIWAFISFPLALLGTVV 420
           KSMILTASLFPFTCFGIGF+LN IA+FYGSLAAIPFGTMVVVFVIWAFISFPL LLGTVV
Sbjct: 361 KSMILTASLFPFTCFGIGFVLNMIAVFYGSLAAIPFGTMVVVFVIWAFISFPLVLLGTVV 420

Query: 421 GRNWSGTPNNPCRVKTIPRPIPEKKWYLTPSVVSMMGGLLPFGSIFIEMYFVFTSFWNYK 480
           GRNWSGT NNPCRVKTIPRPIPEKKWYLTPSVVS+MGGLLPFGSIFIEMYFVFTSFWNYK
Sbjct: 421 GRNWSGTANNPCRVKTIPRPIPEKKWYLTPSVVSLMGGLLPFGSIFIEMYFVFTSFWNYK 480

Query: 481 VYYVYGFMLLVFLILIIVTICVTIVGTYFLLNAENYHWQWTSFFSAASTAVYVYLYSVYY 540
           VYYVYGFMLLVFLILIIVTICVTIVGTYFLLNAENYHWQWTSFFSAASTAVYVYLYS+YY
Sbjct: 481 VYYVYGFMLLVFLILIIVTICVTIVGTYFLLNAENYHWQWTSFFSAASTAVYVYLYSIYY 540

Query: 541 YYVKTKMSGFFQTSFYFGYTLMFCLGLGILCGAVGYLGSNLFVRRIYRNIKCD 548
           YYVKTKMSGFFQTSFYFGYTLMFCLGLGILCGAVGYLGSNLFVRRIY+NIKCD
Sbjct: 541 YYVKTKMSGFFQTSFYFGYTLMFCLGLGILCGAVGYLGSNLFVRRIYKNIKCD 593

BLAST of Spg025914 vs. ExPASy TrEMBL
Match: A0A6J1KEK8 (Transmembrane 9 superfamily member OS=Cucurbita maxima OX=3661 GN=LOC111492532 PE=3 SV=1)

HSP 1 Score: 1010.7 bits (2612), Expect = 2.2e-291
Identity = 515/596 (86.41%), Postives = 530/596 (88.93%), Query Frame = 0

Query: 1   MSSFKRPIPITGPFIFFFLF----LLSPAFAAESDHKYQQGESVVLWVNKVGPYNNPQET 60
           MSS  R IP+TGPFIFFF F     L PA A+ESDHKY  GESVVLWVNKVGPYNNPQET
Sbjct: 1   MSSSVRRIPLTGPFIFFFFFFFLSFLPPALASESDHKYHPGESVVLWVNKVGPYNNPQET 60

Query: 61  YNYYSLPFCHPTGDSAHKWGGLGEVLGGNELIDSRIEIKFQKNVDRTTICQLDLDEAKVK 120
           YNYYSLPFCHP+G+SAHKWGGLGEVLGGNELIDSRIE+KFQ+NVDRTTIC LDLDE  VK
Sbjct: 61  YNYYSLPFCHPSGESAHKWGGLGEVLGGNELIDSRIELKFQRNVDRTTICHLDLDETTVK 120

Query: 121 QFKDAIESNYWLEFFMDDLPLWGFVGELHSDKNSEDEKHVLYTHKNIVVKYNKDQIIHVN 180
           QFKDAI+S+YWLEFFMDDLPLWGFVGELHSDKNSEDEKHVLYTHKNI+VKYNKDQIIHVN
Sbjct: 121 QFKDAIKSSYWLEFFMDDLPLWGFVGELHSDKNSEDEKHVLYTHKNIIVKYNKDQIIHVN 180

Query: 181 LTQESPKSLDVGRSLDMTYSVKWIPTNVTFARRFDIYLDYPFFEHQ-------------- 240
           LTQESPKSL+VGRSLDMTYSVKWIPTNVTF RRFDIYLDYPFFEHQ              
Sbjct: 181 LTQESPKSLEVGRSLDMTYSVKWIPTNVTFTRRFDIYLDYPFFEHQIHWFSIFNSFMMVI 240

Query: 241 -------------------------------ERDVSEESGWKLVHGDVFRSPRGLVILSA 300
                                          ERDV+EESGWKLVHGDVFRSPRGLVILSA
Sbjct: 241 FLTGLVSMILMRTLRNDYAKYAREDDDLETLERDVNEESGWKLVHGDVFRSPRGLVILSA 300

Query: 301 VVGTGAQLAVLVLLVILLAIVGMLYVGRGAIITTFIVCYALTSSLSGYVSGGMYSRHGGK 360
           VVGTGAQLAVLVLLVILLAIV MLYVGRGAIITTFIVCYALTSSLSGYVSGGMYSRHGGK
Sbjct: 301 VVGTGAQLAVLVLLVILLAIVVMLYVGRGAIITTFIVCYALTSSLSGYVSGGMYSRHGGK 360

Query: 361 SWIKSMILTASLFPFTCFGIGFILNTIAIFYGSLAAIPFGTMVVVFVIWAFISFPLALLG 420
           SWIKSMILTASLFPFTCFGIGF+LN IAIFYGSLAAIPFGTMVVVFVIWAFISFPL LLG
Sbjct: 361 SWIKSMILTASLFPFTCFGIGFVLNMIAIFYGSLAAIPFGTMVVVFVIWAFISFPLVLLG 420

Query: 421 TVVGRNWSGTPNNPCRVKTIPRPIPEKKWYLTPSVVSMMGGLLPFGSIFIEMYFVFTSFW 480
           TVVGRNWSGT NNPCRVKTIPRPIPEKKWYLTPSVVS+MGGLLPFGSIFIEMYFVFTSFW
Sbjct: 421 TVVGRNWSGTANNPCRVKTIPRPIPEKKWYLTPSVVSLMGGLLPFGSIFIEMYFVFTSFW 480

Query: 481 NYKVYYVYGFMLLVFLILIIVTICVTIVGTYFLLNAENYHWQWTSFFSAASTAVYVYLYS 540
           NYKVYYVYGFMLLVFLILIIVTICVTIVGTYFLLNAENYHWQWTSFFSAASTAVYVYLYS
Sbjct: 481 NYKVYYVYGFMLLVFLILIIVTICVTIVGTYFLLNAENYHWQWTSFFSAASTAVYVYLYS 540

Query: 541 VYYYYVKTKMSGFFQTSFYFGYTLMFCLGLGILCGAVGYLGSNLFVRRIYRNIKCD 548
           +YYYYVKTKMSGFFQTSFYFGYTLMFCLGLGILCGAVGYLGSNLFVRRIY+NIKCD
Sbjct: 541 IYYYYVKTKMSGFFQTSFYFGYTLMFCLGLGILCGAVGYLGSNLFVRRIYKNIKCD 596

BLAST of Spg025914 vs. ExPASy TrEMBL
Match: A0A6J1G2D7 (Transmembrane 9 superfamily member OS=Cucurbita moschata OX=3662 GN=LOC111450061 PE=3 SV=1)

HSP 1 Score: 1001.5 bits (2588), Expect = 1.4e-288
Identity = 511/596 (85.74%), Postives = 531/596 (89.09%), Query Frame = 0

Query: 1   MSSFKRPIPITGPFI---FFFLFLLSPAFAAESDHKYQQGESVVLWVNKVGPYNNPQETY 60
           MSSF R IP+T PFI   FFFLFLLSPA AAESDHKYQ GESVVLWVNKVGPYNNPQETY
Sbjct: 1   MSSFVRFIPLTWPFIIIFFFFLFLLSPALAAESDHKYQPGESVVLWVNKVGPYNNPQETY 60

Query: 61  NYYSLPFCHPTGDSAHKWGGLGEVLGGNELIDSRIEIKFQKNVDRTTICQLDLDEAKVKQ 120
           NYYSLPFCHP+GDSAHKWGGLGEVLGGNELIDSRIEIKF KN+DRTTIC L LDEAKVK 
Sbjct: 61  NYYSLPFCHPSGDSAHKWGGLGEVLGGNELIDSRIEIKFLKNMDRTTICPLHLDEAKVKL 120

Query: 121 FKDAIESNYWLEFFMDDLPLWGFVGELHSDKNSEDEKHVLYTHKNIVVKYNKDQIIHVNL 180
           FK+AI+ +YWLE F+DDLPLWGFVGEL SDKNSEDEKH+LYTHKNI++KYNKDQIIHVNL
Sbjct: 121 FKNAIQRSYWLELFIDDLPLWGFVGELPSDKNSEDEKHILYTHKNIIIKYNKDQIIHVNL 180

Query: 181 TQESPKSLDVGRSLDMTYSVKWIPTNVTFARRFDIYLDYPFFEHQ--------------- 240
           TQESPKSL+VGRSLDMTYSVKWIPTNVTFARRFDIYLDYPFFEHQ               
Sbjct: 181 TQESPKSLEVGRSLDMTYSVKWIPTNVTFARRFDIYLDYPFFEHQIHWFSIFNSFMMVIF 240

Query: 241 -------------------------------ERDVSEESGWKLVHGDVFRSPRGLVILSA 300
                                          ERDVSEESGWKLVHGDVFRSPR LVILSA
Sbjct: 241 LTGLVSMILMRTLRNDYAKYAREDDDDLETLERDVSEESGWKLVHGDVFRSPRCLVILSA 300

Query: 301 VVGTGAQLAVLVLLVILLAIVGMLYVGRGAIITTFIVCYALTSSLSGYVSGGMYSRHGGK 360
           VVGTGAQLAVLVLLVILLAI+GMLYVGRGAIITTFIVCYALTSSLSGYVSGGMYSRHGGK
Sbjct: 301 VVGTGAQLAVLVLLVILLAIIGMLYVGRGAIITTFIVCYALTSSLSGYVSGGMYSRHGGK 360

Query: 361 SWIKSMILTASLFPFTCFGIGFILNTIAIFYGSLAAIPFGTMVVVFVIWAFISFPLALLG 420
           SWIKSMILTASLFPF CFGIG +LNTIAIFYGSLAAIPFGTMVVVFVIWAFI FPLALLG
Sbjct: 361 SWIKSMILTASLFPFMCFGIGLVLNTIAIFYGSLAAIPFGTMVVVFVIWAFIYFPLALLG 420

Query: 421 TVVGRNWSGTPNNPCRVKTIPRPIPEKKWYLTPSVVSMMGGLLPFGSIFIEMYFVFTSFW 480
           TVVGRNWSGTPNNPCRVKTIPRP+PEKKWYLTPSVVS+MGGLLPFGSIFIEMYFVFTSFW
Sbjct: 421 TVVGRNWSGTPNNPCRVKTIPRPVPEKKWYLTPSVVSLMGGLLPFGSIFIEMYFVFTSFW 480

Query: 481 NYKVYYVYGFMLLVFLILIIVTICVTIVGTYFLLNAENYHWQWTSFFSAASTAVYVYLYS 540
           NYKVYYVYGFMLLVFLILIIVTICVTIVGTYFLLNAENYHWQWTSFFSAASTAVY++LYS
Sbjct: 481 NYKVYYVYGFMLLVFLILIIVTICVTIVGTYFLLNAENYHWQWTSFFSAASTAVYLFLYS 540

Query: 541 VYYYYVKTKMSGFFQTSFYFGYTLMFCLGLGILCGAVGYLGSNLFVRRIYRNIKCD 548
           +YY+YVKT+MSGFFQTSFYFGYTLMFCLGLGILCGAVGYLGSNLFVRRIYRNIKCD
Sbjct: 541 IYYFYVKTRMSGFFQTSFYFGYTLMFCLGLGILCGAVGYLGSNLFVRRIYRNIKCD 596

BLAST of Spg025914 vs. ExPASy TrEMBL
Match: A0A6J1HQG5 (Transmembrane 9 superfamily member OS=Cucurbita maxima OX=3661 GN=LOC111466436 PE=3 SV=1)

HSP 1 Score: 999.6 bits (2583), Expect = 5.1e-288
Identity = 509/597 (85.26%), Postives = 529/597 (88.61%), Query Frame = 0

Query: 1   MSSFKRPIPITGPF-----IFFFLFLLSPAFAAESDHKYQQGESVVLWVNKVGPYNNPQE 60
           MSSF R IP+T PF      FFFLF+LSPA AAESDHKYQ GESVVLWVNKVGPYNNPQE
Sbjct: 1   MSSFVRLIPLTWPFFIIIIFFFFLFVLSPALAAESDHKYQPGESVVLWVNKVGPYNNPQE 60

Query: 61  TYNYYSLPFCHPTGDSAHKWGGLGEVLGGNELIDSRIEIKFQKNVDRTTICQLDLDEAKV 120
           TYNYYSLPFCHP+GDSAHKWGGLGEVLGGNELIDSRIEIKF KN+DRTTIC L LDEAKV
Sbjct: 61  TYNYYSLPFCHPSGDSAHKWGGLGEVLGGNELIDSRIEIKFLKNMDRTTICPLHLDEAKV 120

Query: 121 KQFKDAIESNYWLEFFMDDLPLWGFVGELHSDKNSEDEKHVLYTHKNIVVKYNKDQIIHV 180
           K FK+AI+ +YWLEFF+DDLPLWGFVGEL SDKNSEDEKH+LYTHKNI++KYNKDQIIHV
Sbjct: 121 KLFKNAIQRSYWLEFFIDDLPLWGFVGELPSDKNSEDEKHILYTHKNIIIKYNKDQIIHV 180

Query: 181 NLTQESPKSLDVGRSLDMTYSVKWIPTNVTFARRFDIYLDYPFFEHQ------------- 240
           NLTQESPKSLDVGRSLDMTYSVKWIPTNVTFARRFDIYLDYPFFEHQ             
Sbjct: 181 NLTQESPKSLDVGRSLDMTYSVKWIPTNVTFARRFDIYLDYPFFEHQIHRFSIFNSFMMV 240

Query: 241 --------------------------------ERDVSEESGWKLVHGDVFRSPRGLVILS 300
                                           ERDVSEESGWKLVHGDVFRSPR LVILS
Sbjct: 241 IFLTGLVSMILMRTLRNDYAKYAREDDDLETLERDVSEESGWKLVHGDVFRSPRCLVILS 300

Query: 301 AVVGTGAQLAVLVLLVILLAIVGMLYVGRGAIITTFIVCYALTSSLSGYVSGGMYSRHGG 360
           AVVGTGAQLAVLVLLVILLAI+GMLYVGRGAIITTFI CYALTSSLSGYVSGGMYSRHGG
Sbjct: 301 AVVGTGAQLAVLVLLVILLAIIGMLYVGRGAIITTFIACYALTSSLSGYVSGGMYSRHGG 360

Query: 361 KSWIKSMILTASLFPFTCFGIGFILNTIAIFYGSLAAIPFGTMVVVFVIWAFISFPLALL 420
           KSWIKSMILTASLFPF CFGIG +LNTIAIFYGSLAAIPFGTMVVVFVIWAFI FPLALL
Sbjct: 361 KSWIKSMILTASLFPFMCFGIGLVLNTIAIFYGSLAAIPFGTMVVVFVIWAFIYFPLALL 420

Query: 421 GTVVGRNWSGTPNNPCRVKTIPRPIPEKKWYLTPSVVSMMGGLLPFGSIFIEMYFVFTSF 480
           GTVVGRNWSGTPNNPCRVKTIPRP+PEKKWYLTPSVVS+MGGLLPFGSIFIEMYFVFTSF
Sbjct: 421 GTVVGRNWSGTPNNPCRVKTIPRPVPEKKWYLTPSVVSLMGGLLPFGSIFIEMYFVFTSF 480

Query: 481 WNYKVYYVYGFMLLVFLILIIVTICVTIVGTYFLLNAENYHWQWTSFFSAASTAVYVYLY 540
           WNYKVYYVYGFMLLVFLILIIVTIC TIVGTYFLLNAENYHWQWTSFFSAASTAVY++LY
Sbjct: 481 WNYKVYYVYGFMLLVFLILIIVTICATIVGTYFLLNAENYHWQWTSFFSAASTAVYLFLY 540

Query: 541 SVYYYYVKTKMSGFFQTSFYFGYTLMFCLGLGILCGAVGYLGSNLFVRRIYRNIKCD 548
           S+YY+YVKT+MSGFFQTSFYFGYTLMFCLGLGILCGAVGYLGSNLFVRRIYRNIKCD
Sbjct: 541 SIYYFYVKTRMSGFFQTSFYFGYTLMFCLGLGILCGAVGYLGSNLFVRRIYRNIKCD 597

BLAST of Spg025914 vs. ExPASy TrEMBL
Match: A0A6J1HSR4 (Transmembrane 9 superfamily member OS=Cucurbita maxima OX=3661 GN=LOC111466436 PE=3 SV=1)

HSP 1 Score: 995.0 bits (2571), Expect = 1.3e-286
Identity = 509/598 (85.12%), Postives = 529/598 (88.46%), Query Frame = 0

Query: 1   MSSFKRPIPITGPF-----IFFFLFLLSPAFAAESDHKYQQGESVVLWVNKVGPYNNPQE 60
           MSSF R IP+T PF      FFFLF+LSPA AAESDHKYQ GESVVLWVNKVGPYNNPQE
Sbjct: 1   MSSFVRLIPLTWPFFIIIIFFFFLFVLSPALAAESDHKYQPGESVVLWVNKVGPYNNPQE 60

Query: 61  TYNYYSLPFCHPTGDSAHKWGGLGEVLGGNELIDSRIEIKFQKNVDRTTICQLDLDEAKV 120
           TYNYYSLPFCHP+GDSAHKWGGLGEVLGGNELIDSRIEIKF KN+DRTTIC L LDEAKV
Sbjct: 61  TYNYYSLPFCHPSGDSAHKWGGLGEVLGGNELIDSRIEIKFLKNMDRTTICPLHLDEAKV 120

Query: 121 KQFKDAIESNYWLEFFMDDLPLWGFVGELHSDKNSEDEKHVLYTHKNIVVKYNKDQIIHV 180
           K FK+AI+ +YWLEFF+DDLPLWGFVGEL SDKNSEDEKH+LYTHKNI++KYNKDQIIHV
Sbjct: 121 KLFKNAIQRSYWLEFFIDDLPLWGFVGELPSDKNSEDEKHILYTHKNIIIKYNKDQIIHV 180

Query: 181 NLTQESPKSLDVGRSLDMTYSVKWIPTNVTFARRFDIYLDYPFFEHQ------------- 240
           NLTQESPKSLDVGRSLDMTYSVKWIPTNVTFARRFDIYLDYPFFEHQ             
Sbjct: 181 NLTQESPKSLDVGRSLDMTYSVKWIPTNVTFARRFDIYLDYPFFEHQIHRFSIFNSFMMV 240

Query: 241 --------------------------------ERDVSEESGWKLVHGDVFRSPRGLVILS 300
                                           ERDVSEESGWKLVHGDVFRSPR LVILS
Sbjct: 241 IFLTGLVSMILMRTLRNDYAKYAREDDDLETLERDVSEESGWKLVHGDVFRSPRCLVILS 300

Query: 301 AVVGTGAQLAVLVLLVILLAIVGMLYVGRGAIITTFIVCYALTSSLSGYVSGGMYSRHGG 360
           AVVGTGAQLAVLVLLVILLAI+GMLYVGRGAIITTFI CYALTSSLSGYVSGGMYSRHGG
Sbjct: 301 AVVGTGAQLAVLVLLVILLAIIGMLYVGRGAIITTFIACYALTSSLSGYVSGGMYSRHGG 360

Query: 361 KSWIKSMILTASLFPFTCFGIGFILNTIAIFYGSLAAIPFGTMVVVFVIWAFISFPLALL 420
           KSWIKSMILTASLFPF CFGIG +LNTIAIFYGSLAAIPFGTMVVVFVIWAFI FPLALL
Sbjct: 361 KSWIKSMILTASLFPFMCFGIGLVLNTIAIFYGSLAAIPFGTMVVVFVIWAFIYFPLALL 420

Query: 421 GTVVGRNWSGTPNNPCRVKTIPRPIPEKKWYLTPSVVSMMGGLLPFGSIFIEMYFVFTSF 480
           GTVVGRNWSGTPNNPCRVKTIPRP+PEKKWYLTPSVVS+MGGLLPFGSIFIEMYFVFTSF
Sbjct: 421 GTVVGRNWSGTPNNPCRVKTIPRPVPEKKWYLTPSVVSLMGGLLPFGSIFIEMYFVFTSF 480

Query: 481 WNYK-VYYVYGFMLLVFLILIIVTICVTIVGTYFLLNAENYHWQWTSFFSAASTAVYVYL 540
           WNYK VYYVYGFMLLVFLILIIVTIC TIVGTYFLLNAENYHWQWTSFFSAASTAVY++L
Sbjct: 481 WNYKQVYYVYGFMLLVFLILIIVTICATIVGTYFLLNAENYHWQWTSFFSAASTAVYLFL 540

Query: 541 YSVYYYYVKTKMSGFFQTSFYFGYTLMFCLGLGILCGAVGYLGSNLFVRRIYRNIKCD 548
           YS+YY+YVKT+MSGFFQTSFYFGYTLMFCLGLGILCGAVGYLGSNLFVRRIYRNIKCD
Sbjct: 541 YSIYYFYVKTRMSGFFQTSFYFGYTLMFCLGLGILCGAVGYLGSNLFVRRIYRNIKCD 598

BLAST of Spg025914 vs. TAIR 10
Match: AT1G10950.1 (transmembrane nine 1 )

HSP 1 Score: 929.9 bits (2402), Expect = 9.6e-271
Identity = 463/572 (80.94%), Postives = 496/572 (86.71%), Query Frame = 0

Query: 21  LLSPAFAAESDHKYQQGESVVLWVNKVGPYNNPQETYNYYSLPFCHPTGDSAHKWGGLGE 80
           LL+P FA++SDHKYQ  E V LWVNKVGPYNNPQETYNYYSLPFC P+G++ HKWGGLGE
Sbjct: 18  LLTPTFASDSDHKYQAEEQVTLWVNKVGPYNNPQETYNYYSLPFCRPSGNNVHKWGGLGE 77

Query: 81  VLGGNELIDSRIEIKFQKNVDRTTICQLDLDEAKVKQFKDAIESNYWLEFFMDDLPLWGF 140
           VLGGNELIDS I IKF KNV+R+ IC L+LDEAKVK FKDAIES+YW EFFMDDLPLWGF
Sbjct: 78  VLGGNELIDSEIAIKFMKNVERSVICPLELDEAKVKHFKDAIESSYWFEFFMDDLPLWGF 137

Query: 141 VGELHSDKNSEDEKHVLYTHKNIVVKYNKDQIIHVNLTQESPKSLDVGRSLDMTYSVKWI 200
           VGELH DKNSE+ KHVLYTHKNIVVKYNKDQIIHVNLTQ++P+ L+ G+ +D+TYSV+WI
Sbjct: 138 VGELHPDKNSENGKHVLYTHKNIVVKYNKDQIIHVNLTQDNPRPLEAGKKMDLTYSVQWI 197

Query: 201 PTNVTFARRFDIYLDYPFFEHQ-------------------------------------- 260
           PTNVTFARRFD+YLDYPFFEHQ                                      
Sbjct: 198 PTNVTFARRFDVYLDYPFFEHQIHWFSIFNSFMMVIFLTGLVSMILMRTLRNDYAKYARE 257

Query: 261 -------ERDVSEESGWKLVHGDVFRSPRGLVILSAVVGTGAQLAVLVLLVILLAIVGML 320
                  ERDVSEESGWKLVHGDVFR    LV+LSAVVGTGAQLA+LVLLVIL+AIVG L
Sbjct: 258 DDDLESLERDVSEESGWKLVHGDVFRPASSLVLLSAVVGTGAQLALLVLLVILMAIVGTL 317

Query: 321 YVGRGAIITTFIVCYALTSSLSGYVSGGMYSRHGGKSWIKSMILTASLFPFTCFGIGFIL 380
           YVGRGAI+TTFIVCYALTS +SGYVSGGMYSR GGK WIK M+LTASLFPF CFGIGF+L
Sbjct: 318 YVGRGAIVTTFIVCYALTSFVSGYVSGGMYSRSGGKHWIKCMVLTASLFPFLCFGIGFLL 377

Query: 381 NTIAIFYGSLAAIPFGTMVVVFVIWAFISFPLALLGTVVGRNWSGTPNNPCRVKTIPRPI 440
           NTIAIFYGSLAAIPFGTMVVVFVIW FISFPLALLGTVVGRNWSG PNNPCRVKTIPRPI
Sbjct: 378 NTIAIFYGSLAAIPFGTMVVVFVIWGFISFPLALLGTVVGRNWSGAPNNPCRVKTIPRPI 437

Query: 441 PEKKWYLTPSVVSMMGGLLPFGSIFIEMYFVFTSFWNYKVYYVYGFMLLVFLILIIVTIC 500
           PEKKWYLTPSVVS+MGGLLPFGSIFIEMYFVFTSFWNYKVYYVYGFMLLVF+IL+IVT+C
Sbjct: 438 PEKKWYLTPSVVSLMGGLLPFGSIFIEMYFVFTSFWNYKVYYVYGFMLLVFVILVIVTVC 497

Query: 501 VTIVGTYFLLNAENYHWQWTSFFSAASTAVYVYLYSVYYYYVKTKMSGFFQTSFYFGYTL 548
           VTIVGTYFLLNAENYHWQWTSFFSAASTAVYVYLYS+YYYYVKTKMSGFFQTSFYFGYT+
Sbjct: 498 VTIVGTYFLLNAENYHWQWTSFFSAASTAVYVYLYSIYYYYVKTKMSGFFQTSFYFGYTM 557

BLAST of Spg025914 vs. TAIR 10
Match: AT5G37310.1 (Endomembrane protein 70 protein family )

HSP 1 Score: 409.1 bits (1050), Expect = 5.7e-114
Identity = 218/582 (37.46%), Postives = 334/582 (57.39%), Query Frame = 0

Query: 15  IFFFLFLLSPAFAAESDHKYQQGESVVLWVNKVGPYNNPQETYNYYSLPFCHPTGDSAHK 74
           +F FL+ +SP  +  SDH+Y+ G+ V L+ NKVGP++NP ETY Y+ LPFC  +     K
Sbjct: 13  VFLFLYGVSPVISDGSDHRYKVGDDVPLYANKVGPFHNPSETYRYFDLPFC-SSAPVKEK 72

Query: 75  WGGLGEVLGGNELIDSRIEIKFQKNVDRTTICQLDLDEAKVKQFKDAIESNYWLEFFMDD 134
              LGEVL G+ L+ +  +++F    +    C+  L    V +F+D I  +Y+ + + DD
Sbjct: 73  KEALGEVLNGDRLVSAPYKLEFLGEKNSEVACRKRLSREDVAKFRDVIAKDYYFQMYYDD 132

Query: 135 LPLWGFVGELHSDKNSE--DEKHVLYTHKNIVVKYNKDQIIHVNLTQESPKSLDVGR--- 194
           LP+WGF+G++  +  ++  + K+ L+ H    + YNKD++I + +  +    +D+     
Sbjct: 133 LPIWGFLGKVVKEGKTDPSEYKYYLFNHLQFEIFYNKDRVIEIIVRTDQNFLVDLTEDKE 192

Query: 195 -SLDMTYSVKWIPTNVTFARRFDIYL---------------------------------- 254
             +D TY+V+W  T + F +R + Y                                   
Sbjct: 193 VQVDFTYTVRWKETEIPFEKRMEKYSLASSMPHHLEIHWFSIINSCVTVLLLTGFLATIL 252

Query: 255 ------DYPFFEHQER--DVSEESGWKLVHGDVFRSPRGLVILSAVVGTGAQLAVLVLLV 314
                 D+  + H E   D  EE+GWKL+HGDVFR P+   +L+A +G+G QL  L + +
Sbjct: 253 MRVLKNDFVKYAHDEEAVDDQEETGWKLIHGDVFRFPKHKSLLAAALGSGTQLFTLAVFI 312

Query: 315 ILLAIVGMLY-VGRGAIITTFIVCYALTSSLSGYVSGGMYSRHGGKSWIKSMILTASLFP 374
            +LA+VG+ Y   RGA+ T  +V YALTS ++GY +   Y +  G +W++++ILT SLF 
Sbjct: 313 FMLALVGVFYPYNRGALFTALVVIYALTSGIAGYTAASFYCQLEGTNWVRNVILTGSLFC 372

Query: 375 FTCFGIGFILNTIAIFYGSLAAIPFGTMVVVFVIWAFISFPLALLGTVVGRNWSGTPNNP 434
                    LNT+AI Y + AA+PFGT+VV+F+IWA ++ PL +LG + G+N       P
Sbjct: 373 GPLLITFSFLNTVAIAYQATAALPFGTIVVIFLIWALVTSPLLILGGIAGKNRKSEFQAP 432

Query: 435 CRVKTIPRPIPEKKWYLTPSVVSMMGGLLPFGSIFIEMYFVFTSFWNYKVYYVYGFMLLV 494
           CR    PR IP  +WY        M G LPF +I+IE+Y++F S W +++Y +Y  + +V
Sbjct: 433 CRTTKYPREIPPMRWYRRTLPQMAMAGFLPFSAIYIELYYIFASVWGHRIYTIYSILSIV 492

Query: 495 FLILIIVTICVTIVGTYFLLNAENYHWQWTSFFSAASTAVYVYLYSVYYYYVKTKMSGFF 548
           FLIL+IVT  +T+  TYF L AE++ W W S     ST +++Y Y +YYYY ++ MSGF 
Sbjct: 493 FLILVIVTAFITVALTYFQLAAEDHEWWWRSLLCGGSTGLFIYAYCLYYYYARSDMSGFM 552

BLAST of Spg025914 vs. TAIR 10
Match: AT1G14670.1 (Endomembrane protein 70 protein family )

HSP 1 Score: 408.3 bits (1048), Expect = 9.8e-114
Identity = 217/567 (38.27%), Postives = 326/567 (57.50%), Query Frame = 0

Query: 30  SDHKYQQGESVVLWVNKVGPYNNPQETYNYYSLPFCHPTGDSAHKWGGLGEVLGGNELID 89
           SDH+Y++G++V L+ NKVGP++NP ETY Y+ LPFC P G    K   LGEVL G+ L+ 
Sbjct: 27  SDHRYKEGDTVPLYANKVGPFHNPSETYRYFDLPFCIPEGVKEKK-EALGEVLNGDRLVS 86

Query: 90  SRIEIKFQKNVDRTTICQLDLDEAKVKQFKDAIESNYWLEFFMDDLPLWGFVGELHSDKN 149
           +  ++ F+   +    C   L + +VKQF+ A+E +Y+ + + DDLP+WGF+G++  D  
Sbjct: 87  APYKLNFRDEKESEVYCNKKLSKEEVKQFRKAVEKDYYFQMYYDDLPIWGFIGKVDKDIK 146

Query: 150 SE--DEKHVLYTHKNIVVKYNKDQIIHVNLTQESPKSLDVGR----SLDMTYSVKWIPTN 209
           S+  + K+ LY H    + YNKD++I ++   +    +D+        +  Y+VKW  T 
Sbjct: 147 SDPSEFKYFLYKHIQFEILYNKDRVIEISARMDPHSLVDLTEDKEVDAEFMYTVKWKETE 206

Query: 210 VTFARRFDIY---------LDYPFF---------------------------------EH 269
             F +R + Y         L+  +F                                 + 
Sbjct: 207 TPFEKRMEKYSMSSSLPHHLEIHWFSIINSCVTVLLLTGFLATILMRVLKNDFMKYAQDE 266

Query: 270 QERDVSEESGWKLVHGDVFRSPRGLVILSAVVGTGAQLAVLVLLVILLAIVGMLY-VGRG 329
           +  D  EE+GWK +HGDVFR P    + +A +G+G QL  L + + +LA+VG+ Y   RG
Sbjct: 267 EAADDQEETGWKYIHGDVFRFPTHNSLFAASLGSGTQLFTLTIFIFMLALVGVFYPYNRG 326

Query: 330 AIITTFIVCYALTSSLSGYVSGGMYSRHGGKSWIKSMILTASLFPFTCFGIGFILNTIAI 389
           A+ T  +V YALTS ++GY S   Y +  GKSW+++++LT  LF    F     LNT+AI
Sbjct: 327 ALFTALVVIYALTSGIAGYTSASFYCQLEGKSWVRNLLLTGCLFCGPLFLTFCFLNTVAI 386

Query: 390 FYGSLAAIPFGTMVVVFVIWAFISFPLALLGTVVGRNWSGTPNNPCRVKTIPRPIPEKKW 449
            Y + AA+PFGT+VV+ +IW  ++ PL +LG + G+N       PCR    PR IP   W
Sbjct: 387 TYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGKNSKAEFQAPCRTTKYPREIPPLPW 446

Query: 450 YLTPSVVSMMGGLLPFGSIFIEMYFVFTSFWNYKVYYVYGFMLLVFLILIIVTICVTIVG 509
           Y +      M G LPF +I+IE+Y++F S W +++Y +Y  + +VF+ILIIVT  +T+  
Sbjct: 447 YRSAIPQMAMAGFLPFSAIYIELYYIFASVWGHRIYTIYSILFIVFIILIIVTAFITVAL 506

Query: 510 TYFLLNAENYHWQWTSFFSAASTAVYVYLYSVYYYYVKTKMSGFFQTSFYFGYTLMFCLG 548
           TYF L AE++ W W SF    ST +++Y Y +YYYY ++ MSGF QTSF+FGY    C G
Sbjct: 507 TYFQLAAEDHQWWWRSFLCGGSTGLFIYAYCLYYYYARSDMSGFMQTSFFFGYMACICYG 566

BLAST of Spg025914 vs. TAIR 10
Match: AT2G01970.1 (Endomembrane protein 70 protein family )

HSP 1 Score: 404.8 bits (1039), Expect = 1.1e-112
Identity = 212/567 (37.39%), Postives = 325/567 (57.32%), Query Frame = 0

Query: 30  SDHKYQQGESVVLWVNKVGPYNNPQETYNYYSLPFCHPTGDSAHKWGGLGEVLGGNELID 89
           SDH+Y+ G+SV L+ NKVGP++NP ETY Y+ LPFC P G    K   LGEVL G+ L+ 
Sbjct: 27  SDHRYKDGDSVPLYANKVGPFHNPSETYRYFDLPFCIPEGVKDKK-EALGEVLNGDRLVS 86

Query: 90  SRIEIKFQKNVDRTTICQLDLDEAKVKQFKDAIESNYWLEFFMDDLPLWGFVGELHSDKN 149
           +  ++ F+   D    C+  L   +V+ F+ A+E +Y+ + + DDLP+WGF+G++  +  
Sbjct: 87  APYKLNFRDEKDSEVYCKKKLSREEVEHFRRAVEKDYYFQMYYDDLPIWGFIGKVDKESK 146

Query: 150 SE--DEKHVLYTHKNIVVKYNKDQIIHVNLTQESPKSLDVGR----SLDMTYSVKWIPTN 209
           S+  + K+ LY H    + YNKD++I +N   +    +D+        +  Y+VKW  T 
Sbjct: 147 SDPSEFKYFLYKHIQFEILYNKDRVIEINARMDPHSLVDLTEDKEVDAEFMYTVKWKETE 206

Query: 210 VTFARRFDIY---------LDYPFF---------------------------------EH 269
            +F +R D Y         L+  +F                                 + 
Sbjct: 207 TSFEKRMDKYAMSSSLPHHLEIHWFSIINSCVTVLLLTGFLATILMRVLKNDFMKYAQDE 266

Query: 270 QERDVSEESGWKLVHGDVFRSPRGLVILSAVVGTGAQLAVLVLLVILLAIVGMLY-VGRG 329
           +  D  EE+GWK +HGDVFR P+   + +A +G+G QL  L + + +L++VG+ Y   RG
Sbjct: 267 EAADDQEETGWKYIHGDVFRFPKNKSLFAASLGSGTQLFTLTIFIFMLSLVGVFYPYNRG 326

Query: 330 AIITTFIVCYALTSSLSGYVSGGMYSRHGGKSWIKSMILTASLFPFTCFGIGFILNTIAI 389
           A+ T  +V YALTS ++GY +   Y +  GK+W+++++LT  LF    F     LNT+AI
Sbjct: 327 ALFTALVVIYALTSGIAGYTASSFYCQLEGKNWVRNLLLTGGLFCGPLFLTFCFLNTVAI 386

Query: 390 FYGSLAAIPFGTMVVVFVIWAFISFPLALLGTVVGRNWSGTPNNPCRVKTIPRPIPEKKW 449
            Y + AA+PFGT++V+ +IW  ++ PL +LG + G+N       P R    PR IP   W
Sbjct: 387 AYSATAALPFGTIIVIVLIWTLVTSPLLVLGGIAGKNSKAEFQAPVRTTKYPREIPPLPW 446

Query: 450 YLTPSVVSMMGGLLPFGSIFIEMYFVFTSFWNYKVYYVYGFMLLVFLILIIVTICVTIVG 509
           Y +      M G LPF +I+IE+Y++F S W +++Y +Y  + +VF+IL+IVT  +T+  
Sbjct: 447 YRSAVPQMAMAGFLPFSAIYIELYYIFASVWGHRIYTIYSILFIVFIILLIVTAFITVAL 506

Query: 510 TYFLLNAENYHWQWTSFFSAASTAVYVYLYSVYYYYVKTKMSGFFQTSFYFGYTLMFCLG 548
           TYF L AE++ W W SF    ST +++Y Y +YYYY ++ MSGF QTSF+FGY    C G
Sbjct: 507 TYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYYARSDMSGFMQTSFFFGYMACICYG 566

BLAST of Spg025914 vs. TAIR 10
Match: AT1G08350.2 (Endomembrane protein 70 protein family )

HSP 1 Score: 341.3 bits (874), Expect = 1.5e-93
Identity = 197/567 (34.74%), Postives = 311/567 (54.85%), Query Frame = 0

Query: 30  SDHKYQQGESVVLWVNKVGPYNNPQETYNYYSLPFCHPTGDSAHKWGGLGEVLGGNELID 89
           S + Y  G+ V L+VNKVGP +NP ETY YY LPFC   G    K   LGEVL G+ L+ 
Sbjct: 25  SSNHYNAGDHVPLFVNKVGPLHNPSETYQYYDLPFCR-RGPVIEKQETLGEVLNGDRLMS 84

Query: 90  SRIEIKFQKNVDRTTICQLDLDEAKVKQFKDAIESNYWLEFFMDDLPLWGFVGELHSDKN 149
           S  ++KF+++     +C+  L  + + +F+D I  +Y+ + + DDLPLWGFVG++  D  
Sbjct: 85  SLYKLKFREDKTHFVLCRKRLTSSDIARFRDIIAQDYYFQMYYDDLPLWGFVGKVEGDYF 144

Query: 150 SEDEKHV---LYTHKNIVVKYNKDQIIHVNLTQESPKSLDVGRSLDM----TYSVKWIPT 209
            + EKH    +++H    V YN D++I +N   +    +D+  + ++    TYSV W  T
Sbjct: 145 GQGEKHTKYYIFSHLKFNVLYNADKVIEINSFSDPSYMVDISENTEIDVQFTYSVSWNLT 204

Query: 210 NVTFARRFDIY------------------------------LDYPFFEHQERDV------ 269
           +     R + Y                              + + F  H + ++      
Sbjct: 205 SERSETRMNKYSRASFHPISQKIHFFSFLNSITVVVLLIGLISFLFMRHLKNELRSYSIG 264

Query: 270 ----SEESGWKLVHGDVFRSPRGLVILSAVVGTGAQLAVLVLLVILLAIVGMLY-VGRGA 329
                +E+GWKLVH DVFR PR +  L A++GTG QL +L++ +  LA  G LY   RG 
Sbjct: 265 DEEERKEAGWKLVHSDVFRCPRNISWLCAILGTGTQLLILIIALFALAFTGFLYPYNRGM 324

Query: 330 IITTFIVCYALTSSLSGYVSGGMYSRHGGKSWIKSMILTASLFPFTCFGIGFILNTIAIF 389
           ++T+ ++ Y LTS ++GY S   +S+  G    +S+ L   L+P   F I  +LNT+AI 
Sbjct: 325 LLTSLVIMYTLTSIVAGYTSTSFHSQFEGNKQKRSVRLAGILYPVPFFIILSVLNTVAIT 384

Query: 390 YGSLAAIPFGTMVVVFVIWAFISFPLALLGTVVGRNWSGTP-NNPCRVKTIPRPIPEKKW 449
           YG+ AA+PFGT+V++ +I+  ++ P  +LG V+G  +       P  VK  PR IP + W
Sbjct: 385 YGATAALPFGTIVIIILIFTLLNIPFLMLGGVLGNRFGLLEFQPPSAVKRNPREIPPQNW 444

Query: 450 YLTPSVVSMMGGLLPFGSIFIEMYFVFTSFWNYKVYYVYGFMLLVFLILIIVTICVTIVG 509
           Y        +GG +PF ++ +E + ++ S W +K+Y   G ML  F++LI ++  V I+ 
Sbjct: 445 YRRKLYQVFLGGFVPFSAVVLEWHQLYASLWGFKIYTSPGIMLFTFIVLIFLSSSVGIIL 504

Query: 510 TYFLLNAENYHWQWTSFFSAASTAVYVYLYSVYYYYVKTKMSGFFQTSFYFGYTLMFCLG 548
           TY  L+ E++ W W S      TAV++Y Y V  +Y+++ M+GF Q SFY GYT + C  
Sbjct: 505 TYIQLSGEDHEWWWRSILCGGFTAVFMYGYGV-LFYLRSDMTGFLQLSFYLGYTALLCYA 564