Spg025897 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg025897 vs. NCBI nr
Match: XP_022945486.1 (E3 ubiquitin-protein ligase RGLG2-like [Cucurbita moschata])

HSP 1 Score: 728.0 bits (1878), Expect = 5.1e-206
Identity = 366/415 (88.19%), Postives = 385/415 (92.77%), Query Frame = 0

Query: 1   MGGKNSRESGRREFDSYGSGNSSAWDHYNYPPPSPYVQQHPYAQPYDYDGSQTPQPQPQR 60
           MG KNS+E+ +REFDS G GNSS W + NYPPPSPYVQ++ Y Q +DY GSQT  P PQR
Sbjct: 1   MGSKNSKETDQREFDSDGFGNSSTWGYDNYPPPSPYVQEYSYPQRFDY-GSQT--PLPQR 60

Query: 61  RLDRRYSRIADNYQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG 120
           RLDR+YSRIAD+YQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG
Sbjct: 61  RLDRKYSRIADHYQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG 120

Query: 121 NGQNPYEQAISIIGQSLSAFDEDNLIPCYGFGDASTHDQDVFSFYDDDRICNGFEEVLHR 180
           N QNPYEQAISIIGQ+LSAFDEDN+IPCYGFGDASTHDQ+VFSFYDDDRICNGFEEVLHR
Sbjct: 121 NTQNPYEQAISIIGQTLSAFDEDNMIPCYGFGDASTHDQNVFSFYDDDRICNGFEEVLHR 180

Query: 181 YREIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQKSPLRQVTRSVDTHTG 240
           Y+EIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADG      QVTRSVDT+ G
Sbjct: 181 YKEIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADG------QVTRSVDTNNG 240

Query: 241 QLSSQERKTVDAIVRASEYPLSIILVGVGDGPWDMMKDFDDNIPARAFDNFQFVNFTEIM 300
           QLSSQER TVDAIVRASEYPLSIILVGVGDGPWDMMK+FDDNIPARAFDNFQFVNFTEIM
Sbjct: 241 QLSSQERNTVDAIVRASEYPLSIILVGVGDGPWDMMKNFDDNIPARAFDNFQFVNFTEIM 300

Query: 301 SKNVNSARRQTDFALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPPLYGAAPSNGY 360
           SKNVNS RRQT+FALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPPLY   P N Y
Sbjct: 301 SKNVNSTRRQTEFALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPPLYSTTPFNSY 360

Query: 361 PRTPRSNSFQTRTPSHGRYNQGVGTTPSTSSYYDNQVCPICLTNPKDMAFGCGHQ 416
           PRTPR+NSFQTRTPSHGRYNQGVGTT STSS+YDNQ+CPICLTNPKDMAFGCGHQ
Sbjct: 361 PRTPRANSFQTRTPSHGRYNQGVGTTSSTSSHYDNQICPICLTNPKDMAFGCGHQ 406

BLAST of Spg025897 vs. NCBI nr
Match: XP_022966892.1 (E3 ubiquitin-protein ligase RGLG2-like [Cucurbita maxima])

HSP 1 Score: 727.2 bits (1876), Expect = 8.7e-206
Identity = 367/415 (88.43%), Postives = 385/415 (92.77%), Query Frame = 0

Query: 1   MGGKNSRESGRREFDSYGSGNSSAWDHYNYPPPSPYVQQHPYAQPYDYDGSQTPQPQPQR 60
           MG KNS+E+ +REFDS G GNSS W + NYPPPSPYVQQ+ Y Q  DY GSQT  P PQR
Sbjct: 1   MGSKNSKETDQREFDSDGFGNSSTWGYDNYPPPSPYVQQYSYPQRSDY-GSQT--PLPQR 60

Query: 61  RLDRRYSRIADNYQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG 120
           RLDR+YSRIAD+YQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG
Sbjct: 61  RLDRKYSRIADHYQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG 120

Query: 121 NGQNPYEQAISIIGQSLSAFDEDNLIPCYGFGDASTHDQDVFSFYDDDRICNGFEEVLHR 180
           N QNPYEQAISIIGQ+LSAFDEDN+IPCYGFGDASTHDQ+VFSFYD+DRICNGFEEVLHR
Sbjct: 121 NTQNPYEQAISIIGQTLSAFDEDNMIPCYGFGDASTHDQNVFSFYDNDRICNGFEEVLHR 180

Query: 181 YREIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQKSPLRQVTRSVDTHTG 240
           Y+EIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADG      QVTRSVDT+ G
Sbjct: 181 YKEIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADG------QVTRSVDTNNG 240

Query: 241 QLSSQERKTVDAIVRASEYPLSIILVGVGDGPWDMMKDFDDNIPARAFDNFQFVNFTEIM 300
           QLSSQER TVDAIVRASEYPLSIILVGVGDGPWDMMK+FDDNIPARAFDNFQFVNFTEIM
Sbjct: 241 QLSSQERNTVDAIVRASEYPLSIILVGVGDGPWDMMKNFDDNIPARAFDNFQFVNFTEIM 300

Query: 301 SKNVNSARRQTDFALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPPLYGAAPSNGY 360
           SKNVNS RRQT+FALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPPLY   P NGY
Sbjct: 301 SKNVNSTRRQTEFALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPPLYSMTPFNGY 360

Query: 361 PRTPRSNSFQTRTPSHGRYNQGVGTTPSTSSYYDNQVCPICLTNPKDMAFGCGHQ 416
           PRTPR+NSFQTRTPSHGRYNQGVGTT STSS+YDNQ+CPICLTNPKDMAFGCGHQ
Sbjct: 361 PRTPRANSFQTRTPSHGRYNQGVGTTSSTSSHYDNQICPICLTNPKDMAFGCGHQ 406

BLAST of Spg025897 vs. NCBI nr
Match: XP_023542441.1 (E3 ubiquitin-protein ligase RGLG2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 726.1 bits (1873), Expect = 1.9e-205
Identity = 366/415 (88.19%), Postives = 385/415 (92.77%), Query Frame = 0

Query: 1   MGGKNSRESGRREFDSYGSGNSSAWDHYNYPPPSPYVQQHPYAQPYDYDGSQTPQPQPQR 60
           MG KNS+E+ +REFDS G GNSS W + NYPPPSPYV+Q+ Y Q  DY GSQT  P PQR
Sbjct: 1   MGSKNSKETDQREFDSDGFGNSSTWGYDNYPPPSPYVEQYSYPQRSDY-GSQT--PLPQR 60

Query: 61  RLDRRYSRIADNYQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG 120
           RLDR+YSRIAD+YQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG
Sbjct: 61  RLDRKYSRIADHYQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG 120

Query: 121 NGQNPYEQAISIIGQSLSAFDEDNLIPCYGFGDASTHDQDVFSFYDDDRICNGFEEVLHR 180
           N QNPYEQAISIIGQ+LSAFDEDN+IPCYGFGDASTHDQ+VFSFYD+DRICNGFEEVLHR
Sbjct: 121 NVQNPYEQAISIIGQTLSAFDEDNMIPCYGFGDASTHDQNVFSFYDNDRICNGFEEVLHR 180

Query: 181 YREIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQKSPLRQVTRSVDTHTG 240
           Y+EIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADG      QVTRSVDT+ G
Sbjct: 181 YKEIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADG------QVTRSVDTNNG 240

Query: 241 QLSSQERKTVDAIVRASEYPLSIILVGVGDGPWDMMKDFDDNIPARAFDNFQFVNFTEIM 300
           QLSSQER TVDAIVRASEYPLSIILVGVGDGPWDMMK+FDDNIPARAFDNFQFVNFTEIM
Sbjct: 241 QLSSQERNTVDAIVRASEYPLSIILVGVGDGPWDMMKNFDDNIPARAFDNFQFVNFTEIM 300

Query: 301 SKNVNSARRQTDFALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPPLYGAAPSNGY 360
           SKNVNS RRQT+FALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPPLY   P NGY
Sbjct: 301 SKNVNSTRRQTEFALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPPLYSTTPFNGY 360

Query: 361 PRTPRSNSFQTRTPSHGRYNQGVGTTPSTSSYYDNQVCPICLTNPKDMAFGCGHQ 416
           PRTPR+NSFQTRTPSHGRYNQGVGTT STSS+YDNQ+CPICLTNPKDMAFGCGHQ
Sbjct: 361 PRTPRANSFQTRTPSHGRYNQGVGTTSSTSSHYDNQICPICLTNPKDMAFGCGHQ 406

BLAST of Spg025897 vs. NCBI nr
Match: KAG6573681.1 (E3 ubiquitin-protein ligase RGLG2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 725.3 bits (1871), Expect = 3.3e-205
Identity = 365/415 (87.95%), Postives = 385/415 (92.77%), Query Frame = 0

Query: 1   MGGKNSRESGRREFDSYGSGNSSAWDHYNYPPPSPYVQQHPYAQPYDYDGSQTPQPQPQR 60
           MG +NS+E+ +REFDS G GNSS W + NYPPPSPYVQ++ Y Q  DY GSQT  P PQR
Sbjct: 1   MGSRNSKETDQREFDSDGFGNSSTWGYDNYPPPSPYVQEYSYPQRSDY-GSQT--PLPQR 60

Query: 61  RLDRRYSRIADNYQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG 120
           RLDR+YSRIAD+YQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG
Sbjct: 61  RLDRKYSRIADHYQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG 120

Query: 121 NGQNPYEQAISIIGQSLSAFDEDNLIPCYGFGDASTHDQDVFSFYDDDRICNGFEEVLHR 180
           N QNPYEQAISIIGQ+LSAFDEDN+IPCYGFGDASTHDQ+VFSFYD+DRICNGFEEVLHR
Sbjct: 121 NTQNPYEQAISIIGQTLSAFDEDNMIPCYGFGDASTHDQNVFSFYDNDRICNGFEEVLHR 180

Query: 181 YREIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQKSPLRQVTRSVDTHTG 240
           Y+EIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADG      QVTRSVDT+ G
Sbjct: 181 YKEIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADG------QVTRSVDTNNG 240

Query: 241 QLSSQERKTVDAIVRASEYPLSIILVGVGDGPWDMMKDFDDNIPARAFDNFQFVNFTEIM 300
           QLSSQER TVDAIVRASEYPLSIILVGVGDGPWDMMK+FDDNIPARAFDNFQFVNFTEIM
Sbjct: 241 QLSSQERNTVDAIVRASEYPLSIILVGVGDGPWDMMKNFDDNIPARAFDNFQFVNFTEIM 300

Query: 301 SKNVNSARRQTDFALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPPLYGAAPSNGY 360
           SKNVNS RRQT+FALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPPLY   P NGY
Sbjct: 301 SKNVNSTRRQTEFALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPPLYSTTPFNGY 360

Query: 361 PRTPRSNSFQTRTPSHGRYNQGVGTTPSTSSYYDNQVCPICLTNPKDMAFGCGHQ 416
           PRTPR+NSFQTRTPSHGRYNQGVGTT STSS+YDNQ+CPICLTNPKDMAFGCGHQ
Sbjct: 361 PRTPRANSFQTRTPSHGRYNQGVGTTSSTSSHYDNQICPICLTNPKDMAFGCGHQ 406

BLAST of Spg025897 vs. NCBI nr
Match: XP_008446129.1 (PREDICTED: E3 ubiquitin-protein ligase RGLG2-like [Cucumis melo] >XP_008446130.1 PREDICTED: E3 ubiquitin-protein ligase RGLG2-like [Cucumis melo] >KAA0034249.1 E3 ubiquitin-protein ligase RGLG2-like [Cucumis melo var. makuwa] >TYK15671.1 E3 ubiquitin-protein ligase RGLG2-like [Cucumis melo var. makuwa])

HSP 1 Score: 724.2 bits (1868), Expect = 7.4e-205
Identity = 366/416 (87.98%), Postives = 383/416 (92.07%), Query Frame = 0

Query: 1   MGGKNSRESGRREFDSYGSGNSSAWDHYNYPP--PSPYVQQHPYAQPYDYDGSQTPQPQP 60
           MGGKNSRE G+REF SYGSGNSS WD YNYPP  PSPYVQQ+PY +P  Y GSQTPQ QP
Sbjct: 1   MGGKNSREVGQREFGSYGSGNSSTWDQYNYPPPSPSPYVQQYPYTEPSGY-GSQTPQ-QP 60

Query: 61  QRRLDRRYSRIADNYQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHH 120
           +RRLDR+YSRIADNYQSLDEVTAALA AGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHH
Sbjct: 61  RRRLDRKYSRIADNYQSLDEVTAALANAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHH 120

Query: 121 IGNGQNPYEQAISIIGQSLSAFDEDNLIPCYGFGDASTHDQDVFSFYDDDRICNGFEEVL 180
           IGN  NPYEQAISIIGQ+LSAFDEDNLIPC+GFGDASTHDQDVFSFY DDRICNGFEEVL
Sbjct: 121 IGNEPNPYEQAISIIGQTLSAFDEDNLIPCFGFGDASTHDQDVFSFYHDDRICNGFEEVL 180

Query: 181 HRYREIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQKSPLRQVTRSVDTH 240
           HRYREIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADG      QVTRSVDTH
Sbjct: 181 HRYREIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADG------QVTRSVDTH 240

Query: 241 TGQLSSQERKTVDAIVRASEYPLSIILVGVGDGPWDMMKDFDDNIPARAFDNFQFVNFTE 300
            GQLS QERKT+DAIVRASEYPLSII VGVGDGPWDMMK+FDDNIPARAFDNFQFVNFTE
Sbjct: 241 NGQLSLQERKTIDAIVRASEYPLSIIFVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTE 300

Query: 301 IMSKNVNSARRQTDFALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPPLYGAAPSN 360
           IM KNVN+ RRQT+FALAALMEIPSQYQATLELG+LGR+RGSSPERVPLPPPLY A  S+
Sbjct: 301 IMLKNVNNVRRQTEFALAALMEIPSQYQATLELGILGRQRGSSPERVPLPPPLYSATSSS 360

Query: 361 GYPRTPRSNSFQTRTPSHGRYNQGVGTTPSTSSYYDNQVCPICLTNPKDMAFGCGH 415
           GYPRTP SNSFQTRTPS GRYNQGVGT PS+SS YDNQ+CPIC+TNPKDMAFGCGH
Sbjct: 361 GYPRTPCSNSFQTRTPSLGRYNQGVGTNPSSSSPYDNQLCPICITNPKDMAFGCGH 408

BLAST of Spg025897 vs. ExPASy Swiss-Prot
Match: Q9LY87 (E3 ubiquitin-protein ligase RGLG2 OS=Arabidopsis thaliana OX=3702 GN=RGLG2 PE=1 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 4.1e-150
Identity = 283/448 (63.17%), Postives = 335/448 (74.78%), Query Frame = 0

Query: 1   MGGKNSRESGRRE-----FDSYGSGNSSAW-----------DHYNYPPPSPYVQQHPYAQ 60
           MG  NS+E+ R+        S  S +SS+W           + YNYPPP  Y Q   Y Q
Sbjct: 1   MGTGNSKENWRQSSFRSTSASSASPSSSSWASQQSYPQYGAESYNYPPPPSYAQPPEYTQ 60

Query: 61  P--------------YDYDGSQTPQPQPQRRLDRRYSRIADNYQSLDEVTAALAQAGLES 120
           P              Y    SQ+     ++RL+R+YS+I+D+Y SL++VT ALA+AGLES
Sbjct: 61  PPPPLYSTQPYSAPSYSAPPSQSYGSDNKKRLERKYSKISDDYSSLEQVTEALARAGLES 120

Query: 121 SNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQNPYEQAISIIGQSLSAFDEDNLIPCYG 180
           SNLIVGIDFTKSNEWTG+RSFNR+SLH IG+  NPYEQAI+IIG++L+AFDEDNLIPCYG
Sbjct: 121 SNLIVGIDFTKSNEWTGARSFNRKSLHFIGSSPNPYEQAITIIGRTLAAFDEDNLIPCYG 180

Query: 181 FGDASTHDQDVFSFYDDDRICNGFEEVLHRYREIVPNLRLAGPTSFAPIIEMAMTIVEQT 240
           FGDASTHDQDVFSF  +DR CNGFEEVL RY+EIVP L+LAGPTSFAPII+MAMTIVEQ+
Sbjct: 181 FGDASTHDQDVFSFNSEDRFCNGFEEVLSRYKEIVPQLKLAGPTSFAPIIDMAMTIVEQS 240

Query: 241 GGQYHVLLIIADGQKSPLRQVTRSVDTHTGQLSSQERKTVDAIVRASEYPLSIILVGVGD 300
           GGQYHVL+IIADG      QVTRSVDT  GQLS QE+KTVDAIV+AS+ PLSI+LVGVGD
Sbjct: 241 GGQYHVLVIIADG------QVTRSVDTENGQLSPQEQKTVDAIVQASKLPLSIVLVGVGD 300

Query: 301 GPWDMMKDFDDNIPARAFDNFQFVNFTEIMSKNVNSARRQTDFALAALMEIPSQYQATLE 360
           GPWDMM++FDDNIPARAFDNFQFVNFTEIM+KN   + ++T+FAL+ALMEIP QY+AT+E
Sbjct: 301 GPWDMMREFDDNIPARAFDNFQFVNFTEIMAKNKAQSLKETEFALSALMEIPQQYKATIE 360

Query: 361 LGLLGRRRGSSPERVPLPPPLYGAAPSNGYPRTPRSNSFQTRTPSH---GRYNQGVGTTP 416
           L LLGRR G  PER PLPPP+ G + S   P+  R  SF+   P H   G + +     P
Sbjct: 361 LNLLGRRNGYIPERFPLPPPMRGGSSSYNSPKPSRLPSFKPSVPPHPTEGYHVRSSPVPP 420

BLAST of Spg025897 vs. ExPASy Swiss-Prot
Match: Q8LB88 (E3 ubiquitin-protein ligase RGLG5 OS=Arabidopsis thaliana OX=3702 GN=RGLG5 PE=1 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 1.5e-144
Identity = 274/427 (64.17%), Postives = 324/427 (75.88%), Query Frame = 0

Query: 1   MGGKNSRESGR-----REFDSYGSGN---SSAWDHYNYPPPSPYVQQHPYAQPYD--YDG 60
           MGG +S+ES R     R ++   SG+   SSAWD  +Y         HP A P      G
Sbjct: 1   MGGSSSKESPRGGGSGRRYERSVSGSSSYSSAWDQSSY----YQTPNHPSASPVSSYNSG 60

Query: 61  SQTPQPQPQRRLDRRYSRIADNYQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRS 120
            QTP     + L+R+YSRIADNY+S+DEVTAAL+ AGLESSNLIVGID TKSNEWTG+RS
Sbjct: 61  RQTP-----KNLERKYSRIADNYRSIDEVTAALSHAGLESSNLIVGIDVTKSNEWTGARS 120

Query: 121 FNRRSLHHIGNGQNPYEQAISIIGQSLSAFDEDNLIPCYGFGDASTHDQDVFSFYDDDRI 180
           F R+SLH IG   NPY+QAISIIG++LS FDEDNLIPCYGFGDA+THDQDVFSF  +D  
Sbjct: 121 FGRKSLHFIGTTPNPYQQAISIIGKTLSVFDEDNLIPCYGFGDATTHDQDVFSFNPNDTY 180

Query: 181 CNGFEEVLHRYREIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQKSPLRQ 240
           CNGFEEVL  YREIVP LRL+GPTSFAPIIE AMTIVE++GGQYHVLLIIADG      Q
Sbjct: 181 CNGFEEVLMCYREIVPQLRLSGPTSFAPIIERAMTIVEESGGQYHVLLIIADG------Q 240

Query: 241 VTRSVDTHTGQLSSQERKTVDAIVRASEYPLSIILVGVGDGPWDMMKDFDDNIPARAFDN 300
           VTRSVDT  G  S QE++T+DAIVRASEYPLSI+LVGVGDGPWD M+ FDDNIPARAFDN
Sbjct: 241 VTRSVDTDNGGFSPQEQQTIDAIVRASEYPLSIVLVGVGDGPWDTMRQFDDNIPARAFDN 300

Query: 301 FQFVNFTEIMSKNVNSARRQTDFALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPP 360
           FQFVNFT+IMSKN++ AR++ +FAL+ALMEIPSQY+ATLELGLLG+R G  P+R+ LPPP
Sbjct: 301 FQFVNFTDIMSKNIDPARKEAEFALSALMEIPSQYKATLELGLLGQRTGHCPDRIALPPP 360

Query: 361 LYGAAPSNGYPRTPRSNSFQTRTPSHGRYNQGVGTTPSTSSYYDN--QVCPICLTNPKDM 416
            Y        PRT RS SFQ +      Y+ GV +TP ++++ ++  Q CP+CL + K+M
Sbjct: 361 TYATQSMRNSPRTSRSTSFQNKP-----YDNGVSSTPPSTTHNESQQQFCPVCLVSAKNM 407

BLAST of Spg025897 vs. ExPASy Swiss-Prot
Match: Q9SS90 (E3 ubiquitin-protein ligase RGLG1 OS=Arabidopsis thaliana OX=3702 GN=RGLG1 PE=1 SV=1)

HSP 1 Score: 491.5 bits (1264), Expect = 1.1e-137
Identity = 258/404 (63.86%), Postives = 309/404 (76.49%), Query Frame = 0

Query: 29  NYPPPSPYVQQ--------HPYAQP-YDYDGSQTPQPQP--------QRRLDRRYSRIAD 88
           N PPP  Y           H Y+Q  Y    SQ   P P        +++ DRRYS+I+D
Sbjct: 76  NNPPPPTYAPAPSPASSYGHQYSQEGYASAASQPNYPPPPSQSQVADRKKFDRRYSKISD 135

Query: 89  NYQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQNPYEQAIS 148
           NY SL +V+ AL +AGLESSNLIVGIDFTKSNEWTG++SFNR+SLHH+ N  NPYEQAI+
Sbjct: 136 NYSSLLQVSEALGRAGLESSNLIVGIDFTKSNEWTGAKSFNRKSLHHLSNTPNPYEQAIT 195

Query: 149 IIGQSLSAFDEDNLIPCYGFGDASTHDQDVFSFYDDDRICNGFEEVLHRYREIVPNLRLA 208
           IIG++L+AFDEDNLIPCYGFGDASTHDQDVFSFY + R CNGFEEVL RYREIVP L+LA
Sbjct: 196 IIGRTLAAFDEDNLIPCYGFGDASTHDQDVFSFYPEGRFCNGFEEVLARYREIVPQLKLA 255

Query: 209 GPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQKSPLRQVTRSVDTHTGQLSSQERKTVD 268
           GPTSFAPIIEMAMT+VEQ+ GQYHVL+IIADG      QVTRSVDT  G+LS QE+KTVD
Sbjct: 256 GPTSFAPIIEMAMTVVEQSSGQYHVLVIIADG------QVTRSVDTEHGRLSPQEQKTVD 315

Query: 269 AIVRASEYPLSIILVGVGDGPWDMMKDFDDNIPARAFDNFQFVNFTEIMSKNVNSARRQT 328
           AIV+AS  PLSI+LVGVGDGPWDMM++FDDNIPARAFDNFQFVNFTEIMSKN + +R++T
Sbjct: 316 AIVKASTLPLSIVLVGVGDGPWDMMQEFDDNIPARAFDNFQFVNFTEIMSKNKDQSRKET 375

Query: 329 DFALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPPLYGAAPSNGYPRTPRSNSFQT 388
           +FAL+ALMEIP QY+AT+EL LLG R G+ P+R+PLPPP+   +  +    + R  +F+ 
Sbjct: 376 EFALSALMEIPPQYKATIELNLLGVRNGNIPQRIPLPPPVQSGSSFS----SSRIPNFEP 435

Query: 389 RTPSHGRYNQGVGTTPSTSSYYDNQVCPICLTNPKDMAFGCGHQ 416
             P +   ++        SS  D Q+CPICL+NPK+MAFGCGHQ
Sbjct: 436 SVPPYPFESK------QMSSADDIQLCPICLSNPKNMAFGCGHQ 463

BLAST of Spg025897 vs. ExPASy Swiss-Prot
Match: Q9SAL0 (E3 ubiquitin-protein ligase RGLG4 OS=Arabidopsis thaliana OX=3702 GN=RGLG4 PE=1 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 1.9e-107
Identity = 198/351 (56.41%), Postives = 252/351 (71.79%), Query Frame = 0

Query: 64  RRYSRIADNYQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQ 123
           ++Y+ I D + SLD+V+ AL +AGLESSNLI+G+DFTKSNEWTG  SF+ + LH +G   
Sbjct: 51  KKYALIPDRFSSLDQVSKALREAGLESSNLILGVDFTKSNEWTGKTSFDGKCLHALGETS 110

Query: 124 NPYEQAISIIGQSLSAFDEDNLIPCYGFGDASTHDQDVFSFYDDDRICNGFEEVLHRYRE 183
           NPYE+AI +IGQ+L+ FDEDNLIPC+GFGD++THD++VF F+ D+  C+GFEEVL  Y+ 
Sbjct: 111 NPYEKAIFVIGQTLAPFDEDNLIPCFGFGDSTTHDEEVFGFHSDNSPCHGFEEVLACYKR 170

Query: 184 IVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQKSPLRQVTRSVDTHTGQLS 243
           I PNLRL+GPTS+ P+I+ A+ IVE+  GQ+HVL+I+ADG      QVTR  D   G+LS
Sbjct: 171 IAPNLRLSGPTSYGPLIDAAVDIVEKNNGQFHVLVIVADG------QVTRGTDMAEGELS 230

Query: 244 SQERKTVDAIVRASEYPLSIILVGVGDGPWDMMKDFDDNIPARAFDNFQFVNFTEIMSKN 303
            QE+ T+DAIV AS Y LSI+LVGVGDGPW+ M+ FDD IP R FDNFQFVNFTEIM++N
Sbjct: 231 QQEKTTIDAIVNASSYALSIVLVGVGDGPWEDMRKFDDKIPKREFDNFQFVNFTEIMTRN 290

Query: 304 VNSARRQTDFALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPPLYGAAPSNGYPRT 363
              + ++T FALAALMEIP QYQA +EL LLG++ G +   VP PPP+         P T
Sbjct: 291 SPESAKETAFALAALMEIPFQYQAAIELRLLGKQTGLAKTIVPRPPPI---------PYT 350

Query: 364 PRSNSFQTRTPSHGRYNQGVGTTPSTSSYYDNQVCPICLTNPKDMAFGCGH 415
           P +N+     PS          T S +S    Q CPICLTN KD+AF CGH
Sbjct: 351 PPTNA---ELPS----------TASPASPEQTQSCPICLTNRKDVAFSCGH 373

BLAST of Spg025897 vs. ExPASy Swiss-Prot
Match: Q8RX26 (E3 ubiquitin-protein ligase RGLG3 OS=Arabidopsis thaliana OX=3702 GN=RGLG3 PE=1 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 4.8e-106
Identity = 207/350 (59.14%), Postives = 250/350 (71.43%), Query Frame = 0

Query: 67  SRIADNYQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQNPY 126
           S IAD++ SLD+V  +L +AGLESSNLI+GIDFTKSNEWTG  SFNR+SLH IG  QNPY
Sbjct: 12  SYIADHFSSLDQVITSLREAGLESSNLILGIDFTKSNEWTGRYSFNRKSLHAIGKRQNPY 71

Query: 127 EQAISIIGQSLSAFDEDNLIPCYGFGDASTHDQDVFSFYDDDRICNGFEEVLHRYREIVP 186
           E+AISIIG++LS FDED+LIPC+GFGD +T DQ VFSFY +++ C+G E  + RYREIVP
Sbjct: 72  EKAISIIGRTLSPFDEDDLIPCFGFGDVTTRDQYVFSFYPENKSCDGLENAVKRYREIVP 131

Query: 187 NLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQKSPLRQVTRSVDTHTGQLSSQE 246
           +L+L+GPTSFAP+I+ A+ IVEQ   QYHVL+IIADG      QVTR+ D   G+LS QE
Sbjct: 132 HLKLSGPTSFAPVIDAAINIVEQNNMQYHVLVIIADG------QVTRNPDVPLGRLSPQE 191

Query: 247 RKTVDAIVRASEYPLSIILVGVGDGPWDMMKDFDDNIPARAFDNFQFVNFTEIMSKNVNS 306
             T+++I+ AS YPLSI+LVGVGDGPWD MK FDDNIP R FDNFQFVNFT+IMS++ ++
Sbjct: 192 EATMNSIMAASHYPLSIVLVGVGDGPWDTMKQFDDNIPHREFDNFQFVNFTKIMSEHKDA 251

Query: 307 ARRQTDFALAALMEIPSQYQATLELGLLGRR--RGSSPERVPLPPPLYGAAPSNGYPRTP 366
           A+++  FALAALMEIP QY+ATL    L R+  R S     PLPPP       N     P
Sbjct: 252 AKKEAAFALAALMEIPFQYKATLS---LNRKPVRSSHQHHKPLPPPPEVIERDNAVRSVP 311

Query: 367 RSNSFQTRTPSHGRYNQGVGTTPSTSSYYDNQVCPICLTNPKDMAFGCGH 415
                 T T       +     PST       VCPICLTNPKDMAF CGH
Sbjct: 312 NQ---MTETA-----EKSDRLAPSTV-----PVCPICLTNPKDMAFSCGH 339

BLAST of Spg025897 vs. ExPASy TrEMBL
Match: A0A6J1G0Z9 (E3 ubiquitin-protein ligase RGLG2-like OS=Cucurbita moschata OX=3662 GN=LOC111449704 PE=4 SV=1)

HSP 1 Score: 728.0 bits (1878), Expect = 2.5e-206
Identity = 366/415 (88.19%), Postives = 385/415 (92.77%), Query Frame = 0

Query: 1   MGGKNSRESGRREFDSYGSGNSSAWDHYNYPPPSPYVQQHPYAQPYDYDGSQTPQPQPQR 60
           MG KNS+E+ +REFDS G GNSS W + NYPPPSPYVQ++ Y Q +DY GSQT  P PQR
Sbjct: 1   MGSKNSKETDQREFDSDGFGNSSTWGYDNYPPPSPYVQEYSYPQRFDY-GSQT--PLPQR 60

Query: 61  RLDRRYSRIADNYQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG 120
           RLDR+YSRIAD+YQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG
Sbjct: 61  RLDRKYSRIADHYQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG 120

Query: 121 NGQNPYEQAISIIGQSLSAFDEDNLIPCYGFGDASTHDQDVFSFYDDDRICNGFEEVLHR 180
           N QNPYEQAISIIGQ+LSAFDEDN+IPCYGFGDASTHDQ+VFSFYDDDRICNGFEEVLHR
Sbjct: 121 NTQNPYEQAISIIGQTLSAFDEDNMIPCYGFGDASTHDQNVFSFYDDDRICNGFEEVLHR 180

Query: 181 YREIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQKSPLRQVTRSVDTHTG 240
           Y+EIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADG      QVTRSVDT+ G
Sbjct: 181 YKEIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADG------QVTRSVDTNNG 240

Query: 241 QLSSQERKTVDAIVRASEYPLSIILVGVGDGPWDMMKDFDDNIPARAFDNFQFVNFTEIM 300
           QLSSQER TVDAIVRASEYPLSIILVGVGDGPWDMMK+FDDNIPARAFDNFQFVNFTEIM
Sbjct: 241 QLSSQERNTVDAIVRASEYPLSIILVGVGDGPWDMMKNFDDNIPARAFDNFQFVNFTEIM 300

Query: 301 SKNVNSARRQTDFALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPPLYGAAPSNGY 360
           SKNVNS RRQT+FALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPPLY   P N Y
Sbjct: 301 SKNVNSTRRQTEFALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPPLYSTTPFNSY 360

Query: 361 PRTPRSNSFQTRTPSHGRYNQGVGTTPSTSSYYDNQVCPICLTNPKDMAFGCGHQ 416
           PRTPR+NSFQTRTPSHGRYNQGVGTT STSS+YDNQ+CPICLTNPKDMAFGCGHQ
Sbjct: 361 PRTPRANSFQTRTPSHGRYNQGVGTTSSTSSHYDNQICPICLTNPKDMAFGCGHQ 406

BLAST of Spg025897 vs. ExPASy TrEMBL
Match: A0A6J1HP79 (E3 ubiquitin-protein ligase RGLG2-like OS=Cucurbita maxima OX=3661 GN=LOC111466460 PE=4 SV=1)

HSP 1 Score: 727.2 bits (1876), Expect = 4.2e-206
Identity = 367/415 (88.43%), Postives = 385/415 (92.77%), Query Frame = 0

Query: 1   MGGKNSRESGRREFDSYGSGNSSAWDHYNYPPPSPYVQQHPYAQPYDYDGSQTPQPQPQR 60
           MG KNS+E+ +REFDS G GNSS W + NYPPPSPYVQQ+ Y Q  DY GSQT  P PQR
Sbjct: 1   MGSKNSKETDQREFDSDGFGNSSTWGYDNYPPPSPYVQQYSYPQRSDY-GSQT--PLPQR 60

Query: 61  RLDRRYSRIADNYQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG 120
           RLDR+YSRIAD+YQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG
Sbjct: 61  RLDRKYSRIADHYQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG 120

Query: 121 NGQNPYEQAISIIGQSLSAFDEDNLIPCYGFGDASTHDQDVFSFYDDDRICNGFEEVLHR 180
           N QNPYEQAISIIGQ+LSAFDEDN+IPCYGFGDASTHDQ+VFSFYD+DRICNGFEEVLHR
Sbjct: 121 NTQNPYEQAISIIGQTLSAFDEDNMIPCYGFGDASTHDQNVFSFYDNDRICNGFEEVLHR 180

Query: 181 YREIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQKSPLRQVTRSVDTHTG 240
           Y+EIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADG      QVTRSVDT+ G
Sbjct: 181 YKEIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADG------QVTRSVDTNNG 240

Query: 241 QLSSQERKTVDAIVRASEYPLSIILVGVGDGPWDMMKDFDDNIPARAFDNFQFVNFTEIM 300
           QLSSQER TVDAIVRASEYPLSIILVGVGDGPWDMMK+FDDNIPARAFDNFQFVNFTEIM
Sbjct: 241 QLSSQERNTVDAIVRASEYPLSIILVGVGDGPWDMMKNFDDNIPARAFDNFQFVNFTEIM 300

Query: 301 SKNVNSARRQTDFALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPPLYGAAPSNGY 360
           SKNVNS RRQT+FALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPPLY   P NGY
Sbjct: 301 SKNVNSTRRQTEFALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPPLYSMTPFNGY 360

Query: 361 PRTPRSNSFQTRTPSHGRYNQGVGTTPSTSSYYDNQVCPICLTNPKDMAFGCGHQ 416
           PRTPR+NSFQTRTPSHGRYNQGVGTT STSS+YDNQ+CPICLTNPKDMAFGCGHQ
Sbjct: 361 PRTPRANSFQTRTPSHGRYNQGVGTTSSTSSHYDNQICPICLTNPKDMAFGCGHQ 406

BLAST of Spg025897 vs. ExPASy TrEMBL
Match: A0A1S3BDV1 (E3 ubiquitin-protein ligase RGLG2-like OS=Cucumis melo OX=3656 GN=LOC103488946 PE=4 SV=1)

HSP 1 Score: 724.2 bits (1868), Expect = 3.6e-205
Identity = 366/416 (87.98%), Postives = 383/416 (92.07%), Query Frame = 0

Query: 1   MGGKNSRESGRREFDSYGSGNSSAWDHYNYPP--PSPYVQQHPYAQPYDYDGSQTPQPQP 60
           MGGKNSRE G+REF SYGSGNSS WD YNYPP  PSPYVQQ+PY +P  Y GSQTPQ QP
Sbjct: 1   MGGKNSREVGQREFGSYGSGNSSTWDQYNYPPPSPSPYVQQYPYTEPSGY-GSQTPQ-QP 60

Query: 61  QRRLDRRYSRIADNYQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHH 120
           +RRLDR+YSRIADNYQSLDEVTAALA AGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHH
Sbjct: 61  RRRLDRKYSRIADNYQSLDEVTAALANAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHH 120

Query: 121 IGNGQNPYEQAISIIGQSLSAFDEDNLIPCYGFGDASTHDQDVFSFYDDDRICNGFEEVL 180
           IGN  NPYEQAISIIGQ+LSAFDEDNLIPC+GFGDASTHDQDVFSFY DDRICNGFEEVL
Sbjct: 121 IGNEPNPYEQAISIIGQTLSAFDEDNLIPCFGFGDASTHDQDVFSFYHDDRICNGFEEVL 180

Query: 181 HRYREIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQKSPLRQVTRSVDTH 240
           HRYREIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADG      QVTRSVDTH
Sbjct: 181 HRYREIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADG------QVTRSVDTH 240

Query: 241 TGQLSSQERKTVDAIVRASEYPLSIILVGVGDGPWDMMKDFDDNIPARAFDNFQFVNFTE 300
            GQLS QERKT+DAIVRASEYPLSII VGVGDGPWDMMK+FDDNIPARAFDNFQFVNFTE
Sbjct: 241 NGQLSLQERKTIDAIVRASEYPLSIIFVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTE 300

Query: 301 IMSKNVNSARRQTDFALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPPLYGAAPSN 360
           IM KNVN+ RRQT+FALAALMEIPSQYQATLELG+LGR+RGSSPERVPLPPPLY A  S+
Sbjct: 301 IMLKNVNNVRRQTEFALAALMEIPSQYQATLELGILGRQRGSSPERVPLPPPLYSATSSS 360

Query: 361 GYPRTPRSNSFQTRTPSHGRYNQGVGTTPSTSSYYDNQVCPICLTNPKDMAFGCGH 415
           GYPRTP SNSFQTRTPS GRYNQGVGT PS+SS YDNQ+CPIC+TNPKDMAFGCGH
Sbjct: 361 GYPRTPCSNSFQTRTPSLGRYNQGVGTNPSSSSPYDNQLCPICITNPKDMAFGCGH 408

BLAST of Spg025897 vs. ExPASy TrEMBL
Match: A0A5D3CUT6 (E3 ubiquitin-protein ligase RGLG2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001680 PE=4 SV=1)

HSP 1 Score: 724.2 bits (1868), Expect = 3.6e-205
Identity = 366/416 (87.98%), Postives = 383/416 (92.07%), Query Frame = 0

Query: 1   MGGKNSRESGRREFDSYGSGNSSAWDHYNYPP--PSPYVQQHPYAQPYDYDGSQTPQPQP 60
           MGGKNSRE G+REF SYGSGNSS WD YNYPP  PSPYVQQ+PY +P  Y GSQTPQ QP
Sbjct: 1   MGGKNSREVGQREFGSYGSGNSSTWDQYNYPPPSPSPYVQQYPYTEPSGY-GSQTPQ-QP 60

Query: 61  QRRLDRRYSRIADNYQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHH 120
           +RRLDR+YSRIADNYQSLDEVTAALA AGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHH
Sbjct: 61  RRRLDRKYSRIADNYQSLDEVTAALANAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHH 120

Query: 121 IGNGQNPYEQAISIIGQSLSAFDEDNLIPCYGFGDASTHDQDVFSFYDDDRICNGFEEVL 180
           IGN  NPYEQAISIIGQ+LSAFDEDNLIPC+GFGDASTHDQDVFSFY DDRICNGFEEVL
Sbjct: 121 IGNEPNPYEQAISIIGQTLSAFDEDNLIPCFGFGDASTHDQDVFSFYHDDRICNGFEEVL 180

Query: 181 HRYREIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQKSPLRQVTRSVDTH 240
           HRYREIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADG      QVTRSVDTH
Sbjct: 181 HRYREIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADG------QVTRSVDTH 240

Query: 241 TGQLSSQERKTVDAIVRASEYPLSIILVGVGDGPWDMMKDFDDNIPARAFDNFQFVNFTE 300
            GQLS QERKT+DAIVRASEYPLSII VGVGDGPWDMMK+FDDNIPARAFDNFQFVNFTE
Sbjct: 241 NGQLSLQERKTIDAIVRASEYPLSIIFVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTE 300

Query: 301 IMSKNVNSARRQTDFALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPPLYGAAPSN 360
           IM KNVN+ RRQT+FALAALMEIPSQYQATLELG+LGR+RGSSPERVPLPPPLY A  S+
Sbjct: 301 IMLKNVNNVRRQTEFALAALMEIPSQYQATLELGILGRQRGSSPERVPLPPPLYSATSSS 360

Query: 361 GYPRTPRSNSFQTRTPSHGRYNQGVGTTPSTSSYYDNQVCPICLTNPKDMAFGCGH 415
           GYPRTP SNSFQTRTPS GRYNQGVGT PS+SS YDNQ+CPIC+TNPKDMAFGCGH
Sbjct: 361 GYPRTPCSNSFQTRTPSLGRYNQGVGTNPSSSSPYDNQLCPICITNPKDMAFGCGH 408

BLAST of Spg025897 vs. ExPASy TrEMBL
Match: A0A6J1DFL9 (E3 ubiquitin-protein ligase RGLG2-like OS=Momordica charantia OX=3673 GN=LOC111019656 PE=4 SV=1)

HSP 1 Score: 715.3 bits (1845), Expect = 1.7e-202
Identity = 355/415 (85.54%), Postives = 382/415 (92.05%), Query Frame = 0

Query: 1   MGGKNSRESGRREFDSYGSGNSSAWDHYNYPPPSPYVQQHPYAQPYDYDGSQTPQPQPQR 60
           MGG+NS+E  RREFDSYG  NS+AWD ++YPPPSPY QQ+PY    DY GSQT  PQPQR
Sbjct: 1   MGGRNSKEPSRREFDSYGYDNSAAWDQFSYPPPSPYPQQYPYTPSSDY-GSQT--PQPQR 60

Query: 61  RLDRRYSRIADNYQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG 120
           RLDR+YSRIADNY SLDEVT+ALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG
Sbjct: 61  RLDRKYSRIADNYSSLDEVTSALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG 120

Query: 121 NGQNPYEQAISIIGQSLSAFDEDNLIPCYGFGDASTHDQDVFSFYDDDRICNGFEEVLHR 180
           NGQNPYEQAISIIGQ+LS FDEDNLIPC+GFGDASTHDQDVFSFY+ DR CNGFEEVLHR
Sbjct: 121 NGQNPYEQAISIIGQTLSVFDEDNLIPCFGFGDASTHDQDVFSFYEHDRTCNGFEEVLHR 180

Query: 181 YREIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQKSPLRQVTRSVDTHTG 240
           YRE+VPNLRLAGPTSFAPI+EMA TIVE+TGGQYHVLLIIADG      QVTRSVDTH G
Sbjct: 181 YREVVPNLRLAGPTSFAPIVEMATTIVEKTGGQYHVLLIIADG------QVTRSVDTHHG 240

Query: 241 QLSSQERKTVDAIVRASEYPLSIILVGVGDGPWDMMKDFDDNIPARAFDNFQFVNFTEIM 300
           QLS QERKT+DAIV+ASEYPLSIILVGVGDGPWDMMK+FDDNIPARAFDNFQFVNFTEIM
Sbjct: 241 QLSPQERKTIDAIVKASEYPLSIILVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEIM 300

Query: 301 SKNVNSARRQTDFALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPPLYGAAPSNGY 360
           SKNVN +RRQT+FALAALMEIPSQY+ATLELGLLGR+RGSSPERVPLPPPLYGAAPSNGY
Sbjct: 301 SKNVNPSRRQTEFALAALMEIPSQYKATLELGLLGRQRGSSPERVPLPPPLYGAAPSNGY 360

Query: 361 PRTPRSNSFQTRTPSHGRYNQGVGTTPSTSSYYDNQVCPICLTNPKDMAFGCGHQ 416
            RT  SNSFQ+RTP+H R N+GVGT PS+SS+YDNQ+CPICLTNPKDMAFGCGHQ
Sbjct: 361 SRTSHSNSFQSRTPAHSRNNKGVGTAPSSSSHYDNQLCPICLTNPKDMAFGCGHQ 406

BLAST of Spg025897 vs. TAIR 10
Match: AT5G14420.1 (RING domain ligase2 )

HSP 1 Score: 532.7 bits (1371), Expect = 2.9e-151
Identity = 283/448 (63.17%), Postives = 335/448 (74.78%), Query Frame = 0

Query: 1   MGGKNSRESGRRE-----FDSYGSGNSSAW-----------DHYNYPPPSPYVQQHPYAQ 60
           MG  NS+E+ R+        S  S +SS+W           + YNYPPP  Y Q   Y Q
Sbjct: 1   MGTGNSKENWRQSSFRSTSASSASPSSSSWASQQSYPQYGAESYNYPPPPSYAQPPEYTQ 60

Query: 61  P--------------YDYDGSQTPQPQPQRRLDRRYSRIADNYQSLDEVTAALAQAGLES 120
           P              Y    SQ+     ++RL+R+YS+I+D+Y SL++VT ALA+AGLES
Sbjct: 61  PPPPLYSTQPYSAPSYSAPPSQSYGSDNKKRLERKYSKISDDYSSLEQVTEALARAGLES 120

Query: 121 SNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQNPYEQAISIIGQSLSAFDEDNLIPCYG 180
           SNLIVGIDFTKSNEWTG+RSFNR+SLH IG+  NPYEQAI+IIG++L+AFDEDNLIPCYG
Sbjct: 121 SNLIVGIDFTKSNEWTGARSFNRKSLHFIGSSPNPYEQAITIIGRTLAAFDEDNLIPCYG 180

Query: 181 FGDASTHDQDVFSFYDDDRICNGFEEVLHRYREIVPNLRLAGPTSFAPIIEMAMTIVEQT 240
           FGDASTHDQDVFSF  +DR CNGFEEVL RY+EIVP L+LAGPTSFAPII+MAMTIVEQ+
Sbjct: 181 FGDASTHDQDVFSFNSEDRFCNGFEEVLSRYKEIVPQLKLAGPTSFAPIIDMAMTIVEQS 240

Query: 241 GGQYHVLLIIADGQKSPLRQVTRSVDTHTGQLSSQERKTVDAIVRASEYPLSIILVGVGD 300
           GGQYHVL+IIADG      QVTRSVDT  GQLS QE+KTVDAIV+AS+ PLSI+LVGVGD
Sbjct: 241 GGQYHVLVIIADG------QVTRSVDTENGQLSPQEQKTVDAIVQASKLPLSIVLVGVGD 300

Query: 301 GPWDMMKDFDDNIPARAFDNFQFVNFTEIMSKNVNSARRQTDFALAALMEIPSQYQATLE 360
           GPWDMM++FDDNIPARAFDNFQFVNFTEIM+KN   + ++T+FAL+ALMEIP QY+AT+E
Sbjct: 301 GPWDMMREFDDNIPARAFDNFQFVNFTEIMAKNKAQSLKETEFALSALMEIPQQYKATIE 360

Query: 361 LGLLGRRRGSSPERVPLPPPLYGAAPSNGYPRTPRSNSFQTRTPSH---GRYNQGVGTTP 416
           L LLGRR G  PER PLPPP+ G + S   P+  R  SF+   P H   G + +     P
Sbjct: 361 LNLLGRRNGYIPERFPLPPPMRGGSSSYNSPKPSRLPSFKPSVPPHPTEGYHVRSSPVPP 420

BLAST of Spg025897 vs. TAIR 10
Match: AT5G14420.3 (RING domain ligase2 )

HSP 1 Score: 532.7 bits (1371), Expect = 2.9e-151
Identity = 283/448 (63.17%), Postives = 335/448 (74.78%), Query Frame = 0

Query: 1   MGGKNSRESGRRE-----FDSYGSGNSSAW-----------DHYNYPPPSPYVQQHPYAQ 60
           MG  NS+E+ R+        S  S +SS+W           + YNYPPP  Y Q   Y Q
Sbjct: 1   MGTGNSKENWRQSSFRSTSASSASPSSSSWASQQSYPQYGAESYNYPPPPSYAQPPEYTQ 60

Query: 61  P--------------YDYDGSQTPQPQPQRRLDRRYSRIADNYQSLDEVTAALAQAGLES 120
           P              Y    SQ+     ++RL+R+YS+I+D+Y SL++VT ALA+AGLES
Sbjct: 61  PPPPLYSTQPYSAPSYSAPPSQSYGSDNKKRLERKYSKISDDYSSLEQVTEALARAGLES 120

Query: 121 SNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQNPYEQAISIIGQSLSAFDEDNLIPCYG 180
           SNLIVGIDFTKSNEWTG+RSFNR+SLH IG+  NPYEQAI+IIG++L+AFDEDNLIPCYG
Sbjct: 121 SNLIVGIDFTKSNEWTGARSFNRKSLHFIGSSPNPYEQAITIIGRTLAAFDEDNLIPCYG 180

Query: 181 FGDASTHDQDVFSFYDDDRICNGFEEVLHRYREIVPNLRLAGPTSFAPIIEMAMTIVEQT 240
           FGDASTHDQDVFSF  +DR CNGFEEVL RY+EIVP L+LAGPTSFAPII+MAMTIVEQ+
Sbjct: 181 FGDASTHDQDVFSFNSEDRFCNGFEEVLSRYKEIVPQLKLAGPTSFAPIIDMAMTIVEQS 240

Query: 241 GGQYHVLLIIADGQKSPLRQVTRSVDTHTGQLSSQERKTVDAIVRASEYPLSIILVGVGD 300
           GGQYHVL+IIADG      QVTRSVDT  GQLS QE+KTVDAIV+AS+ PLSI+LVGVGD
Sbjct: 241 GGQYHVLVIIADG------QVTRSVDTENGQLSPQEQKTVDAIVQASKLPLSIVLVGVGD 300

Query: 301 GPWDMMKDFDDNIPARAFDNFQFVNFTEIMSKNVNSARRQTDFALAALMEIPSQYQATLE 360
           GPWDMM++FDDNIPARAFDNFQFVNFTEIM+KN   + ++T+FAL+ALMEIP QY+AT+E
Sbjct: 301 GPWDMMREFDDNIPARAFDNFQFVNFTEIMAKNKAQSLKETEFALSALMEIPQQYKATIE 360

Query: 361 LGLLGRRRGSSPERVPLPPPLYGAAPSNGYPRTPRSNSFQTRTPSH---GRYNQGVGTTP 416
           L LLGRR G  PER PLPPP+ G + S   P+  R  SF+   P H   G + +     P
Sbjct: 361 LNLLGRRNGYIPERFPLPPPMRGGSSSYNSPKPSRLPSFKPSVPPHPTEGYHVRSSPVPP 420

BLAST of Spg025897 vs. TAIR 10
Match: AT5G14420.4 (RING domain ligase2 )

HSP 1 Score: 532.7 bits (1371), Expect = 2.9e-151
Identity = 283/448 (63.17%), Postives = 335/448 (74.78%), Query Frame = 0

Query: 1   MGGKNSRESGRRE-----FDSYGSGNSSAW-----------DHYNYPPPSPYVQQHPYAQ 60
           MG  NS+E+ R+        S  S +SS+W           + YNYPPP  Y Q   Y Q
Sbjct: 1   MGTGNSKENWRQSSFRSTSASSASPSSSSWASQQSYPQYGAESYNYPPPPSYAQPPEYTQ 60

Query: 61  P--------------YDYDGSQTPQPQPQRRLDRRYSRIADNYQSLDEVTAALAQAGLES 120
           P              Y    SQ+     ++RL+R+YS+I+D+Y SL++VT ALA+AGLES
Sbjct: 61  PPPPLYSTQPYSAPSYSAPPSQSYGSDNKKRLERKYSKISDDYSSLEQVTEALARAGLES 120

Query: 121 SNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQNPYEQAISIIGQSLSAFDEDNLIPCYG 180
           SNLIVGIDFTKSNEWTG+RSFNR+SLH IG+  NPYEQAI+IIG++L+AFDEDNLIPCYG
Sbjct: 121 SNLIVGIDFTKSNEWTGARSFNRKSLHFIGSSPNPYEQAITIIGRTLAAFDEDNLIPCYG 180

Query: 181 FGDASTHDQDVFSFYDDDRICNGFEEVLHRYREIVPNLRLAGPTSFAPIIEMAMTIVEQT 240
           FGDASTHDQDVFSF  +DR CNGFEEVL RY+EIVP L+LAGPTSFAPII+MAMTIVEQ+
Sbjct: 181 FGDASTHDQDVFSFNSEDRFCNGFEEVLSRYKEIVPQLKLAGPTSFAPIIDMAMTIVEQS 240

Query: 241 GGQYHVLLIIADGQKSPLRQVTRSVDTHTGQLSSQERKTVDAIVRASEYPLSIILVGVGD 300
           GGQYHVL+IIADG      QVTRSVDT  GQLS QE+KTVDAIV+AS+ PLSI+LVGVGD
Sbjct: 241 GGQYHVLVIIADG------QVTRSVDTENGQLSPQEQKTVDAIVQASKLPLSIVLVGVGD 300

Query: 301 GPWDMMKDFDDNIPARAFDNFQFVNFTEIMSKNVNSARRQTDFALAALMEIPSQYQATLE 360
           GPWDMM++FDDNIPARAFDNFQFVNFTEIM+KN   + ++T+FAL+ALMEIP QY+AT+E
Sbjct: 301 GPWDMMREFDDNIPARAFDNFQFVNFTEIMAKNKAQSLKETEFALSALMEIPQQYKATIE 360

Query: 361 LGLLGRRRGSSPERVPLPPPLYGAAPSNGYPRTPRSNSFQTRTPSH---GRYNQGVGTTP 416
           L LLGRR G  PER PLPPP+ G + S   P+  R  SF+   P H   G + +     P
Sbjct: 361 LNLLGRRNGYIPERFPLPPPMRGGSSSYNSPKPSRLPSFKPSVPPHPTEGYHVRSSPVPP 420

BLAST of Spg025897 vs. TAIR 10
Match: AT5G14420.2 (RING domain ligase2 )

HSP 1 Score: 532.7 bits (1371), Expect = 2.9e-151
Identity = 283/448 (63.17%), Postives = 335/448 (74.78%), Query Frame = 0

Query: 1   MGGKNSRESGRRE-----FDSYGSGNSSAW-----------DHYNYPPPSPYVQQHPYAQ 60
           MG  NS+E+ R+        S  S +SS+W           + YNYPPP  Y Q   Y Q
Sbjct: 1   MGTGNSKENWRQSSFRSTSASSASPSSSSWASQQSYPQYGAESYNYPPPPSYAQPPEYTQ 60

Query: 61  P--------------YDYDGSQTPQPQPQRRLDRRYSRIADNYQSLDEVTAALAQAGLES 120
           P              Y    SQ+     ++RL+R+YS+I+D+Y SL++VT ALA+AGLES
Sbjct: 61  PPPPLYSTQPYSAPSYSAPPSQSYGSDNKKRLERKYSKISDDYSSLEQVTEALARAGLES 120

Query: 121 SNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQNPYEQAISIIGQSLSAFDEDNLIPCYG 180
           SNLIVGIDFTKSNEWTG+RSFNR+SLH IG+  NPYEQAI+IIG++L+AFDEDNLIPCYG
Sbjct: 121 SNLIVGIDFTKSNEWTGARSFNRKSLHFIGSSPNPYEQAITIIGRTLAAFDEDNLIPCYG 180

Query: 181 FGDASTHDQDVFSFYDDDRICNGFEEVLHRYREIVPNLRLAGPTSFAPIIEMAMTIVEQT 240
           FGDASTHDQDVFSF  +DR CNGFEEVL RY+EIVP L+LAGPTSFAPII+MAMTIVEQ+
Sbjct: 181 FGDASTHDQDVFSFNSEDRFCNGFEEVLSRYKEIVPQLKLAGPTSFAPIIDMAMTIVEQS 240

Query: 241 GGQYHVLLIIADGQKSPLRQVTRSVDTHTGQLSSQERKTVDAIVRASEYPLSIILVGVGD 300
           GGQYHVL+IIADG      QVTRSVDT  GQLS QE+KTVDAIV+AS+ PLSI+LVGVGD
Sbjct: 241 GGQYHVLVIIADG------QVTRSVDTENGQLSPQEQKTVDAIVQASKLPLSIVLVGVGD 300

Query: 301 GPWDMMKDFDDNIPARAFDNFQFVNFTEIMSKNVNSARRQTDFALAALMEIPSQYQATLE 360
           GPWDMM++FDDNIPARAFDNFQFVNFTEIM+KN   + ++T+FAL+ALMEIP QY+AT+E
Sbjct: 301 GPWDMMREFDDNIPARAFDNFQFVNFTEIMAKNKAQSLKETEFALSALMEIPQQYKATIE 360

Query: 361 LGLLGRRRGSSPERVPLPPPLYGAAPSNGYPRTPRSNSFQTRTPSH---GRYNQGVGTTP 416
           L LLGRR G  PER PLPPP+ G + S   P+  R  SF+   P H   G + +     P
Sbjct: 361 LNLLGRRNGYIPERFPLPPPMRGGSSSYNSPKPSRLPSFKPSVPPHPTEGYHVRSSPVPP 420

BLAST of Spg025897 vs. TAIR 10
Match: AT1G67800.1 (Copine (Calcium-dependent phospholipid-binding protein) family )

HSP 1 Score: 514.2 bits (1323), Expect = 1.1e-145
Identity = 274/427 (64.17%), Postives = 324/427 (75.88%), Query Frame = 0

Query: 1   MGGKNSRESGR-----REFDSYGSGN---SSAWDHYNYPPPSPYVQQHPYAQPYD--YDG 60
           MGG +S+ES R     R ++   SG+   SSAWD  +Y         HP A P      G
Sbjct: 1   MGGSSSKESPRGGGSGRRYERSVSGSSSYSSAWDQSSY----YQTPNHPSASPVSSYNSG 60

Query: 61  SQTPQPQPQRRLDRRYSRIADNYQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRS 120
            QTP     + L+R+YSRIADNY+S+DEVTAAL+ AGLESSNLIVGID TKSNEWTG+RS
Sbjct: 61  RQTP-----KNLERKYSRIADNYRSIDEVTAALSHAGLESSNLIVGIDVTKSNEWTGARS 120

Query: 121 FNRRSLHHIGNGQNPYEQAISIIGQSLSAFDEDNLIPCYGFGDASTHDQDVFSFYDDDRI 180
           F R+SLH IG   NPY+QAISIIG++LS FDEDNLIPCYGFGDA+THDQDVFSF  +D  
Sbjct: 121 FGRKSLHFIGTTPNPYQQAISIIGKTLSVFDEDNLIPCYGFGDATTHDQDVFSFNPNDTY 180

Query: 181 CNGFEEVLHRYREIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQKSPLRQ 240
           CNGFEEVL  YREIVP LRL+GPTSFAPIIE AMTIVE++GGQYHVLLIIADG      Q
Sbjct: 181 CNGFEEVLMCYREIVPQLRLSGPTSFAPIIERAMTIVEESGGQYHVLLIIADG------Q 240

Query: 241 VTRSVDTHTGQLSSQERKTVDAIVRASEYPLSIILVGVGDGPWDMMKDFDDNIPARAFDN 300
           VTRSVDT  G  S QE++T+DAIVRASEYPLSI+LVGVGDGPWD M+ FDDNIPARAFDN
Sbjct: 241 VTRSVDTDNGGFSPQEQQTIDAIVRASEYPLSIVLVGVGDGPWDTMRQFDDNIPARAFDN 300

Query: 301 FQFVNFTEIMSKNVNSARRQTDFALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPP 360
           FQFVNFT+IMSKN++ AR++ +FAL+ALMEIPSQY+ATLELGLLG+R G  P+R+ LPPP
Sbjct: 301 FQFVNFTDIMSKNIDPARKEAEFALSALMEIPSQYKATLELGLLGQRTGHCPDRIALPPP 360

Query: 361 LYGAAPSNGYPRTPRSNSFQTRTPSHGRYNQGVGTTPSTSSYYDN--QVCPICLTNPKDM 416
            Y        PRT RS SFQ +      Y+ GV +TP ++++ ++  Q CP+CL + K+M
Sbjct: 361 TYATQSMRNSPRTSRSTSFQNKP-----YDNGVSSTPPSTTHNESQQQFCPVCLVSAKNM 407