Spg025874 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg025874 vs. NCBI nr
Match: CAN75028.1 (hypothetical protein VITISV_026823 [Vitis vinifera])

HSP 1 Score: 370.9 bits (951), Expect = 1.7e-98
Identity = 191/501 (38.12%), Postives = 282/501 (56.29%), Query Frame = 0

Query: 1    MQKTGIVGINMEEGEVVRMADLLGCKAEGLPFNYWGVTVDGRPRVASFWDPVLEAIRAKL 60
            + K+ I GIN+++  + R+A++L CKA G P  Y G+ + G P+   FWDPV+E I ++L
Sbjct: 1347 LNKSSIYGINLDQAHLSRLAEMLDCKASGWPILYLGLPLGGNPKACGFWDPVVERISSRL 1406

Query: 61   AIWKGFPISKGGRTTLIKSVLSSMPVYFFSIFRIPVGVAKEIEKLFRRFLWKG-GDGIRG 120
              W+   +S GGR TLI+S LS +P YF S+F++P  VA +IE+L R FLW G G+G R 
Sbjct: 1407 DGWQKAYLSXGGRITLIQSCLSHLPSYFLSLFKMPASVAAKIERLQRDFLWSGVGEGKR- 1466

Query: 121  SHLVRWDFVTTPLSLRGLGIDDLRAKNAALLAKWLWRFETEPDAMWRRVILSKYG---NN 180
             HLVRWD V  P ++ GLG+ ++  +N ALL KWLWR+  E  A+W +VILS YG   N 
Sbjct: 1467 DHLVRWDIVCKPKTIGGLGLGNISWRNLALLGKWLWRYPREGSALWHQVILSIYGSHSNG 1526

Query: 181  FIRNHLASKRWKSSSSPWRDIASLAGFFKNHIRYKVGRGDKVMFWEDIWLSDRPLKDRFP 240
            +  N L   RW S   PW+ IA +   F    RY VG GD++ FWED+W  D+PL  ++P
Sbjct: 1527 WDANTLV--RW-SHRCPWKAIAQVFQEFSLITRYVVGNGDRIRFWEDLWRGDQPLGIQYP 1586

Query: 241  CIYRLSMAKKAKVCELWNGS---TWNLNMRRGIFDRESSSWIEMVSLLS--HFNPNGNED 300
             ++R+ + K   +  +   S    WNLN RR + D E      ++  L   + +P+   D
Sbjct: 1587 RLFRVVVDKNISISSVLGPSRPFLWNLNFRRNLSDSEIEDLEGLMRSLDDLYLSPS-IPD 1646

Query: 301  LVQWDLEASGLFSIKSALSVLQQRRRLLEEDLCTKIWEGPIPKRVKFFLWTLALNGINTM 360
               W L +SGLFS+KS    L Q     +      +W   +P +VK F+W +A   +NT 
Sbjct: 1647 ARLWPLSSSGLFSVKSFFLALSQSSGSSQNFPSKFVWNSQVPFKVKSFVWLVAHKKVNTN 1706

Query: 361  DKVQSRFPSLQISPNVCVMCMKQEESASHLLLHCEFAAKVWNKFEEIFGIQTCNPFSVVS 420
            D +Q R P   +SPN+C++CMK  ES  HL LHC     +W +  ++  +    P S+  
Sbjct: 1707 DMLQVRRPYKALSPNICILCMKHGESTDHLFLHCSLMIGLWYRLFQLAKMDWVPPRSIYD 1766

Query: 421  WLKEALEGGGLKGKARVLWRNSTRAIIWHLWRERNSRIFADKSLPFNIFCDIVQLSASNW 480
             +    +G G   +  VLW+ ++ A+I  +W ERN++IF DK+    +  D +   AS W
Sbjct: 1767 MMSIKFKGFGNSKRGIVLWQAASIALIRVVWWERNAKIFEDKARNSEVLWDSIVFLASLW 1826

Query: 481  SALDKVFSNYSVAMIHLQWKA 493
            +   K F    + +IHL W A
Sbjct: 1827 AFCSKAFKGIPLNVIHLDWIA 1842

BLAST of Spg025874 vs. NCBI nr
Match: RVW27524.1 (putative ribonuclease H protein [Vitis vinifera])

HSP 1 Score: 369.8 bits (948), Expect = 3.7e-98
Identity = 191/496 (38.51%), Postives = 278/496 (56.05%), Query Frame = 0

Query: 1    MQKTGIVGINMEEGEVVRMADLLGCKAEGLPFNYWGVTVDGRPRVASFWDPVLEAIRAKL 60
            + K+ I GIN+ +  + R+A+LL CKA G P  Y G+ + G P+ +SFWDPV+E I ++L
Sbjct: 526  LDKSNIYGINLGQDHLHRLAELLDCKASGWPILYLGLPLGGNPKSSSFWDPVIERISSRL 585

Query: 61   AIWKGFPISKGGRTTLIKSVLSSMPVYFFSIFRIPVGVAKEIEKLFRRFLWKG-GDGIRG 120
              W+   +S GGR TLI+S L+ MP YF S+F+IP  VA  IE+L R FLW G G+G R 
Sbjct: 586  DGWQKAYLSFGGRITLIRSCLTHMPCYFLSLFKIPASVAVRIERLQRDFLWSGVGEGKR- 645

Query: 121  SHLVRWDFVTTPLSLRGLGIDDLRAKNAALLAKWLWRFETEPDAMWRRVILSKYG---NN 180
             HLV W+ V       GLG+  +  +N+ALL KWLWR+  E  A+W +VILS YG   N 
Sbjct: 646  DHLVSWEVVCKSKMKGGLGLGMISLRNSALLGKWLWRYPREGSALWHQVILSIYGSHSNG 705

Query: 181  FIRNHLASKRWKSSSSPWRDIASLAGFFKNHIRYKVGRGDKVMFWEDIWLSDRPLKDRFP 240
            +  N +   RW S   PW+ IA +   F    R+ VG GD++ FWED+W  D+ L  RFP
Sbjct: 706  WDANTVV--RW-SHRCPWKAIAQVFQDFSKFTRFMVGDGDRIRFWEDLWWGDQSLGVRFP 765

Query: 241  CIYRLSMAKKAKVCELWNGS---TWNLNMRRGIFDRESSSWIEMVSLLSHFNPNGN-EDL 300
             + R+ M K   +  +   +   +WN N RR + D E      ++  L H + + +  D 
Sbjct: 766  RLLRVVMDKNIPISSILGSTRPFSWNFNFRRNLSDSEIEELESLMQSLDHLHLSPSIPDK 825

Query: 301  VQWDLEASGLFSIKSALSVLQQRRRLLEEDLCTKIWEGPIPKRVKFFLWTLALNGINTMD 360
              W L +SGLF++KS    L Q   L        +W   +P ++KFF+W +A   +NT D
Sbjct: 826  RSWSLSSSGLFTVKSFFLALSQISGLPLVFPTKLVWNSQVPFKIKFFVWLVAHKKVNTSD 885

Query: 361  KVQSRFPSLQISPNVCVMCMKQEESASHLLLHCEFAAKVWNKFEEIFGIQTCNPFSVVSW 420
             +Q R P   +SP++C++CM++ E+  HL LHC     +W++  ++  I    P SV   
Sbjct: 886  MLQLRRPYKALSPDICMLCMERGETVDHLFLHCSMTMGLWHRLFQLTKIDWVPPRSVFDM 945

Query: 421  LKEALEGGGLKGKARVLWRNSTRAIIWHLWRERNSRIFADKSLPFNIFCDIVQLSASNWS 480
            +     G G   +  VLW+ +  AI+W +WRERN+RIF DKS       D++   AS W 
Sbjct: 946  ISINFNGFGSSKRGIVLWQAACIAILWVVWRERNARIFEDKSRNSENLWDMIHFLASLWV 1005

Query: 481  ALDKVFSNYSVAMIHL 489
            +  KVF    + +IHL
Sbjct: 1006 SCSKVFKGIPLNVIHL 1017

BLAST of Spg025874 vs. NCBI nr
Match: CAN68165.1 (hypothetical protein VITISV_008538 [Vitis vinifera])

HSP 1 Score: 368.6 bits (945), Expect = 8.3e-98
Identity = 191/496 (38.51%), Postives = 276/496 (55.65%), Query Frame = 0

Query: 1    MQKTGIVGINMEEGEVVRMADLLGCKAEGLPFNYWGVTVDGRPRVASFWDPVLEAIRAKL 60
            + K+ I GIN+ +  + R+A+LL CKA G P  Y G+ + G P+  SFWDPV+E I ++L
Sbjct: 832  LDKSNIYGINLGQDHLHRLAELLDCKASGWPILYLGLPLGGNPKSGSFWDPVIERISSRL 891

Query: 61   AIWKGFPISKGGRTTLIKSVLSSMPVYFFSIFRIPVGVAKEIEKLFRRFLWKG-GDGIRG 120
              W+   +S GGR TLI+S L+ MP YF S+F+IP  VA  IE+L R FLW G G+G R 
Sbjct: 892  DGWQKAYLSFGGRITLIQSCLTHMPCYFLSLFKIPASVAGRIERLQRDFLWSGVGEGKR- 951

Query: 121  SHLVRWDFVTTPLSLRGLGIDDLRAKNAALLAKWLWRFETEPDAMWRRVILSKYG---NN 180
             HLV W+ V       GLG+  +  +N+ALL KWLWR+  E  A+W +VILS YG   N 
Sbjct: 952  DHLVSWBVVCKSKMKGGLGLGRISLRNSALLGKWLWRYPREGSALWHQVILSIYGSHSNG 1011

Query: 181  FIRNHLASKRWKSSSSPWRDIASLAGFFKNHIRYKVGRGDKVMFWEDIWLSDRPLKDRFP 240
            +  N     RW S   PW+ IA +   F    R+ VG GD++ FWED+W  D+ L  RFP
Sbjct: 1012 WDAN--TXVRW-SHRCPWKAIAQVFQDFSKFTRFIVGDGDRIRFWEDLWWGDQSLGVRFP 1071

Query: 241  CIYRLSMAKKAKVCELWNGS---TWNLNMRRGIFDRESSSWIEMVSLLSHFNPNGN-EDL 300
             + R+ M K   +  +   +   +WN N RR + D E      ++  L H + + +  D 
Sbjct: 1072 RLLRVVMDKNILISSILGSTRPFSWNFNFRRNLSDSEIEKVESLMQSLDHIHLSPSVPDK 1131

Query: 301  VQWDLEASGLFSIKSALSVLQQRRRLLEEDLCTKIWEGPIPKRVKFFLWTLALNGINTMD 360
              W L +SGLF++KS    L Q   L        +W   +P ++KFF+W +A   +NT D
Sbjct: 1132 RSWSLSSSGLFTVKSFFLALSQISGLPSVFPTKLVWNSQVPFKIKFFVWLVAHKKVNTND 1191

Query: 361  KVQSRFPSLQISPNVCVMCMKQEESASHLLLHCEFAAKVWNKFEEIFGIQTCNPFSVVSW 420
             +Q R P   +SP++C++CM++ E+  HL LHC     +W++  ++  I    P SV   
Sbjct: 1192 MLQLRRPYKALSPDICMLCMERGETVDHLFLHCSMTMGLWHRLFQLTKIDWVPPRSVFDM 1251

Query: 421  LKEALEGGGLKGKARVLWRNSTRAIIWHLWRERNSRIFADKSLPFNIFCDIVQLSASNWS 480
            +     G G   +  VLW+ +  AI+W +WRERN+RIF DKS       D++   AS W 
Sbjct: 1252 ISINFNGFGSSKRGIVLWQAACIAILWVVWRERNARIFEDKSRNSENLWDMIHFLASLWV 1311

Query: 481  ALDKVFSNYSVAMIHL 489
            +  KVF    + +IHL
Sbjct: 1312 SCSKVFKGIPLNVIHL 1323

BLAST of Spg025874 vs. NCBI nr
Match: CAN62051.1 (hypothetical protein VITISV_016641 [Vitis vinifera])

HSP 1 Score: 367.5 bits (942), Expect = 1.8e-97
Identity = 188/502 (37.45%), Postives = 276/502 (54.98%), Query Frame = 0

Query: 1    MQKTGIVGINMEEGEVVRMADLLGCKAEGLPFNYWGVTVDGRPRVASFWDPVLEAIRAKL 60
            + K+ I GIN+E+  + R+A +L CKA G P  Y G+ + G P+ + FWDPV+E I  +L
Sbjct: 1368 LDKSNIYGINIEQNHLSRLAVMLDCKASGWPILYLGLPLGGNPKASGFWDPVIERISRRL 1427

Query: 61   AIWKGFPISKGGRTTLIKSVLSSMPVYFFSIFRIPVGVAKEIEKLFRRFLWKG-GDGIRG 120
              W+   +S GGR TLI+S L+ MP YF S+FRIP  VA +IE++ R FLW G G+G R 
Sbjct: 1428 DGWQKAYLSFGGRITLIQSCLTHMPCYFLSLFRIPASVAAKIERMQREFLWSGVGEGKR- 1487

Query: 121  SHLVRWDFVTTPLSLRGLGIDDLRAKNAALLAKWLWRFETEPDAMWRRVILSKYGNNF-- 180
             HLV WD V  P S  GLG   +  +N ALL KWLWR+  E  A+W +VILS YG++   
Sbjct: 1488 DHLVNWDVVCKPKSRGGLGFGKISMRNVALLGKWLWRYPREGSALWHQVILSIYGSHSNG 1547

Query: 181  --IRNHLASKRWKSSSSPWRDIASLAGFFKNHIRYKVGRGDKVMFWEDIWLSDRPLKDRF 240
              + N++   RW S   PW+ IA +   F    R+ VG GD++ FW+D+W  D+PL  ++
Sbjct: 1548 WDVNNNV---RW-SHRCPWKAIALVFQEFSKFTRFVVGDGDRIRFWDDLWWGDQPLGTQY 1607

Query: 241  PCIYRLSMAKKAKVCELWNGS---TWNLNMRRGIFDRESSSWIEMVSLLS--HFNPNGNE 300
            P +  +   K A +  +   S   +WN N RR + D E      ++  L   H +P+   
Sbjct: 1608 PRLLSVVTDKNALISSIJGYSRPFSWNFNFRRNLTDSEIEDLESLMRSLDRLHISPS-VX 1667

Query: 301  DLVQWDLEASGLFSIKSALSVLQQRRRLLEEDLCTKIWEGPIPKRVKFFLWTLALNGINT 360
            D   W +  SGLF++KS    L Q            +W   +P +VK F+W +A   +NT
Sbjct: 1668 DKRSWSISPSGLFTVKSFFLALSQXXEXPPVFPTKFVWNSQVPFKVKSFVWLVAHKKLNT 1727

Query: 361  MDKVQSRFPSLQISPNVCVMCMKQEESASHLLLHCEFAAKVWNKFEEIFGIQTCNPFSVV 420
             D +Q R P   +SPN+C +CMK  E+  HL LHC     +W++  ++  +   +P S+ 
Sbjct: 1728 NDLLQLRRPHKALSPNICKLCMKHGETVDHLFLHCSLTKGLWHRLFQLAKMDWVSPRSIS 1787

Query: 421  SWLKEALEGGGLKGKARVLWRNSTRAIIWHLWRERNSRIFADKSLPFNIFCDIVQLSASN 480
                    G G   +  VLW+++  A++W +WRERN+RIF DK+       D ++  AS 
Sbjct: 1788 DMFFTNFNGFGSSKRGVVLWQDACIALMWVVWRERNARIFEDKARNSGCLWDSIRFLASL 1847

Query: 481  WSALDKVFSNYSVAMIHLQWKA 493
            W+   KVF    + M+ L W A
Sbjct: 1848 WAFCSKVFKGIPLNMLQLDWLA 1863

BLAST of Spg025874 vs. NCBI nr
Match: RVW53010.1 (LINE-1 retrotransposable element ORF2 protein [Vitis vinifera])

HSP 1 Score: 367.5 bits (942), Expect = 1.8e-97
Identity = 188/502 (37.45%), Postives = 276/502 (54.98%), Query Frame = 0

Query: 1    MQKTGIVGINMEEGEVVRMADLLGCKAEGLPFNYWGVTVDGRPRVASFWDPVLEAIRAKL 60
            + K+ I GIN+E+  + R+A +L CKA G P  Y G+ + G P+ + FWDPV+E I  +L
Sbjct: 1200 LDKSNIYGINIEQNHLSRLAVMLDCKASGWPILYLGLPLGGNPKASGFWDPVIERISRRL 1259

Query: 61   AIWKGFPISKGGRTTLIKSVLSSMPVYFFSIFRIPVGVAKEIEKLFRRFLWKG-GDGIRG 120
              W+   +S GGR TLI+S L+ MP YF S+FRIP  VA +IE++ R FLW G G+G R 
Sbjct: 1260 DGWQKAYLSFGGRITLIQSCLTHMPCYFLSLFRIPASVAAKIERMQREFLWSGVGEGKR- 1319

Query: 121  SHLVRWDFVTTPLSLRGLGIDDLRAKNAALLAKWLWRFETEPDAMWRRVILSKYGNNF-- 180
             HLV WD V  P S  GLG   +  +N ALL KWLWR+  E  A+W +VILS YG++   
Sbjct: 1320 DHLVNWDVVCKPKSRGGLGFGKISMRNVALLGKWLWRYPREGSALWHQVILSIYGSHSNG 1379

Query: 181  --IRNHLASKRWKSSSSPWRDIASLAGFFKNHIRYKVGRGDKVMFWEDIWLSDRPLKDRF 240
              + N++   RW S   PW+ IA +   F    R+ VG GD++ FW+D+W  D+PL  ++
Sbjct: 1380 WDVNNNV---RW-SHRCPWKAIALVFQEFSKFTRFVVGDGDRIRFWDDLWWGDQPLGTQY 1439

Query: 241  PCIYRLSMAKKAKVCELWNGS---TWNLNMRRGIFDRESSSWIEMVSLLS--HFNPNGNE 300
            P +  +   K A +  +   S   +WN N RR + D E      ++  L   H +P+   
Sbjct: 1440 PRLLSVVTDKNALISSILGYSRPFSWNFNFRRNLTDSEIEDLESLMRSLDRLHISPS-VP 1499

Query: 301  DLVQWDLEASGLFSIKSALSVLQQRRRLLEEDLCTKIWEGPIPKRVKFFLWTLALNGINT 360
            D   W +  SGLF++KS    L Q            +W   +P +VK F+W +A   +NT
Sbjct: 1500 DKRSWSISPSGLFTVKSFFLALSQHSESPPVFPTKFVWNSQVPFKVKSFVWLVAHKKLNT 1559

Query: 361  MDKVQSRFPSLQISPNVCVMCMKQEESASHLLLHCEFAAKVWNKFEEIFGIQTCNPFSVV 420
             D +Q R P   +SPN+C +CMK  E+  HL LHC     +W++  ++  +   +P S+ 
Sbjct: 1560 NDLLQLRRPHKALSPNICKLCMKHGETVDHLFLHCSLTKGLWHRLFQLAKMDWVSPRSIS 1619

Query: 421  SWLKEALEGGGLKGKARVLWRNSTRAIIWHLWRERNSRIFADKSLPFNIFCDIVQLSASN 480
                    G G   +  VLW+++  A++W +WRERN+RIF DK+       D ++  AS 
Sbjct: 1620 DMFFTNFNGFGSSKRGVVLWQDACIALMWVVWRERNARIFEDKARNSGCLWDSIRFLASL 1679

Query: 481  WSALDKVFSNYSVAMIHLQWKA 493
            W+   KVF    + M+ L W A
Sbjct: 1680 WAFCSKVFKGIPLNMLQLDWLA 1695

BLAST of Spg025874 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 6.3e-40
Identity = 118/417 (28.30%), Postives = 184/417 (44.12%), Query Frame = 0

Query: 52  VLEAIRAKLAIWKGFPISKGGRTTLIKSVLSSMPVYFFSIFRIPVGVAKEIEKLFRRFLW 111
           +LE + ++++ W+   +S  GR TL K+VLSSMPV+  S   +P  +   +++L R FLW
Sbjct: 16  ILERVSSRMSGWREKTLSFAGRLTLTKAVLSSMPVHSMSTILLPQSILNRLDQLSRTFLW 75

Query: 112 KGGDGIRGSHLVRWDFVTTPLSLRGLGIDDLRAKNAALLAKWLWRFETEPDAMWRRVILS 171
                 +  HLV+W  V +P    GLG+   ++ N AL++K  WR   E +++W  V+  
Sbjct: 76  GSTAEKKKQHLVKWSKVCSPKKEGGLGVRAAKSMNRALISKVGWRLLQEKNSLWTLVLQK 135

Query: 172 KYGNNFIRNHLASKRW----KSSSSPWRDIA-SLAGFFKNHIRYKVGRGDKVMFWEDIWL 231
           KY    IR+     RW     S SS WR IA  L     + + +  G G ++ FW D W+
Sbjct: 136 KYHVGEIRD----SRWLIPKGSWSSTWRSIAIGLRDVVSHGVGWIPGDGQQIRFWTDRWV 195

Query: 232 SDRPLKDRFPCIYRLSMAKKAKVC------ELW-NGSTWNLNMRRGIFDRESSSWIEMVS 291
           S +PL +       L   ++   C      +LW  G  W+           +   +  V 
Sbjct: 196 SGKPLLE-------LDNGERPTDCDTVVAKDLWIPGRGWDFAKIDPYTTNNTRLELRAVV 255

Query: 292 LLSHFNPNGNEDLVQWDLEASGLFSIKSALSVL--QQRRRLLEEDLCTKIWEGPIPKRVK 351
           L       G  D + W     G FS++SA  +L   +  R         +W+  +P+RVK
Sbjct: 256 L---DLVTGARDRLSWKFSQDGQFSVRSAYEMLTVDEVPRPNMASFFNCLWKVRVPERVK 315

Query: 352 FFLWTLALNGINTMDKVQSRFPSLQISPNVCVMCMKQEESASHLLLHCEFAAKVWNKFEE 411
            FLW +    + T ++   R  S   + NVC +C    ES  H+L  C     +W +   
Sbjct: 316 TFLWLVGNQAVMTEEERHRRHLS---ASNVCQVCKGGVESMLHVLRDCPAQLGIWVRVVP 375

Query: 412 IFGIQTCNPFSVVSWLKEALEGGGLKGKARVLWRNSTRAIIWHLWRERNSRIFADKS 455
               Q     S+  WL + L  G   G   + W      IIW  W+ R   IF + +
Sbjct: 376 QRRQQGFFSKSLFEWLYDNL--GDRSGCEDIPWSTIFAVIIWWGWKWRCGNIFGENT 413

BLAST of Spg025874 vs. ExPASy TrEMBL
Match: A5BUT3 (Reverse transcriptase domain-containing protein OS=Vitis vinifera OX=29760 GN=VITISV_026823 PE=4 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 8.1e-99
Identity = 191/501 (38.12%), Postives = 282/501 (56.29%), Query Frame = 0

Query: 1    MQKTGIVGINMEEGEVVRMADLLGCKAEGLPFNYWGVTVDGRPRVASFWDPVLEAIRAKL 60
            + K+ I GIN+++  + R+A++L CKA G P  Y G+ + G P+   FWDPV+E I ++L
Sbjct: 1347 LNKSSIYGINLDQAHLSRLAEMLDCKASGWPILYLGLPLGGNPKACGFWDPVVERISSRL 1406

Query: 61   AIWKGFPISKGGRTTLIKSVLSSMPVYFFSIFRIPVGVAKEIEKLFRRFLWKG-GDGIRG 120
              W+   +S GGR TLI+S LS +P YF S+F++P  VA +IE+L R FLW G G+G R 
Sbjct: 1407 DGWQKAYLSXGGRITLIQSCLSHLPSYFLSLFKMPASVAAKIERLQRDFLWSGVGEGKR- 1466

Query: 121  SHLVRWDFVTTPLSLRGLGIDDLRAKNAALLAKWLWRFETEPDAMWRRVILSKYG---NN 180
             HLVRWD V  P ++ GLG+ ++  +N ALL KWLWR+  E  A+W +VILS YG   N 
Sbjct: 1467 DHLVRWDIVCKPKTIGGLGLGNISWRNLALLGKWLWRYPREGSALWHQVILSIYGSHSNG 1526

Query: 181  FIRNHLASKRWKSSSSPWRDIASLAGFFKNHIRYKVGRGDKVMFWEDIWLSDRPLKDRFP 240
            +  N L   RW S   PW+ IA +   F    RY VG GD++ FWED+W  D+PL  ++P
Sbjct: 1527 WDANTLV--RW-SHRCPWKAIAQVFQEFSLITRYVVGNGDRIRFWEDLWRGDQPLGIQYP 1586

Query: 241  CIYRLSMAKKAKVCELWNGS---TWNLNMRRGIFDRESSSWIEMVSLLS--HFNPNGNED 300
             ++R+ + K   +  +   S    WNLN RR + D E      ++  L   + +P+   D
Sbjct: 1587 RLFRVVVDKNISISSVLGPSRPFLWNLNFRRNLSDSEIEDLEGLMRSLDDLYLSPS-IPD 1646

Query: 301  LVQWDLEASGLFSIKSALSVLQQRRRLLEEDLCTKIWEGPIPKRVKFFLWTLALNGINTM 360
               W L +SGLFS+KS    L Q     +      +W   +P +VK F+W +A   +NT 
Sbjct: 1647 ARLWPLSSSGLFSVKSFFLALSQSSGSSQNFPSKFVWNSQVPFKVKSFVWLVAHKKVNTN 1706

Query: 361  DKVQSRFPSLQISPNVCVMCMKQEESASHLLLHCEFAAKVWNKFEEIFGIQTCNPFSVVS 420
            D +Q R P   +SPN+C++CMK  ES  HL LHC     +W +  ++  +    P S+  
Sbjct: 1707 DMLQVRRPYKALSPNICILCMKHGESTDHLFLHCSLMIGLWYRLFQLAKMDWVPPRSIYD 1766

Query: 421  WLKEALEGGGLKGKARVLWRNSTRAIIWHLWRERNSRIFADKSLPFNIFCDIVQLSASNW 480
             +    +G G   +  VLW+ ++ A+I  +W ERN++IF DK+    +  D +   AS W
Sbjct: 1767 MMSIKFKGFGNSKRGIVLWQAASIALIRVVWWERNAKIFEDKARNSEVLWDSIVFLASLW 1826

Query: 481  SALDKVFSNYSVAMIHLQWKA 493
            +   K F    + +IHL W A
Sbjct: 1827 AFCSKAFKGIPLNVIHLDWIA 1842

BLAST of Spg025874 vs. ExPASy TrEMBL
Match: A0A438CWE0 (Putative ribonuclease H protein OS=Vitis vinifera OX=29760 GN=VvCHDh000004_278 PE=4 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 1.8e-98
Identity = 191/496 (38.51%), Postives = 278/496 (56.05%), Query Frame = 0

Query: 1    MQKTGIVGINMEEGEVVRMADLLGCKAEGLPFNYWGVTVDGRPRVASFWDPVLEAIRAKL 60
            + K+ I GIN+ +  + R+A+LL CKA G P  Y G+ + G P+ +SFWDPV+E I ++L
Sbjct: 526  LDKSNIYGINLGQDHLHRLAELLDCKASGWPILYLGLPLGGNPKSSSFWDPVIERISSRL 585

Query: 61   AIWKGFPISKGGRTTLIKSVLSSMPVYFFSIFRIPVGVAKEIEKLFRRFLWKG-GDGIRG 120
              W+   +S GGR TLI+S L+ MP YF S+F+IP  VA  IE+L R FLW G G+G R 
Sbjct: 586  DGWQKAYLSFGGRITLIRSCLTHMPCYFLSLFKIPASVAVRIERLQRDFLWSGVGEGKR- 645

Query: 121  SHLVRWDFVTTPLSLRGLGIDDLRAKNAALLAKWLWRFETEPDAMWRRVILSKYG---NN 180
             HLV W+ V       GLG+  +  +N+ALL KWLWR+  E  A+W +VILS YG   N 
Sbjct: 646  DHLVSWEVVCKSKMKGGLGLGMISLRNSALLGKWLWRYPREGSALWHQVILSIYGSHSNG 705

Query: 181  FIRNHLASKRWKSSSSPWRDIASLAGFFKNHIRYKVGRGDKVMFWEDIWLSDRPLKDRFP 240
            +  N +   RW S   PW+ IA +   F    R+ VG GD++ FWED+W  D+ L  RFP
Sbjct: 706  WDANTVV--RW-SHRCPWKAIAQVFQDFSKFTRFMVGDGDRIRFWEDLWWGDQSLGVRFP 765

Query: 241  CIYRLSMAKKAKVCELWNGS---TWNLNMRRGIFDRESSSWIEMVSLLSHFNPNGN-EDL 300
             + R+ M K   +  +   +   +WN N RR + D E      ++  L H + + +  D 
Sbjct: 766  RLLRVVMDKNIPISSILGSTRPFSWNFNFRRNLSDSEIEELESLMQSLDHLHLSPSIPDK 825

Query: 301  VQWDLEASGLFSIKSALSVLQQRRRLLEEDLCTKIWEGPIPKRVKFFLWTLALNGINTMD 360
              W L +SGLF++KS    L Q   L        +W   +P ++KFF+W +A   +NT D
Sbjct: 826  RSWSLSSSGLFTVKSFFLALSQISGLPLVFPTKLVWNSQVPFKIKFFVWLVAHKKVNTSD 885

Query: 361  KVQSRFPSLQISPNVCVMCMKQEESASHLLLHCEFAAKVWNKFEEIFGIQTCNPFSVVSW 420
             +Q R P   +SP++C++CM++ E+  HL LHC     +W++  ++  I    P SV   
Sbjct: 886  MLQLRRPYKALSPDICMLCMERGETVDHLFLHCSMTMGLWHRLFQLTKIDWVPPRSVFDM 945

Query: 421  LKEALEGGGLKGKARVLWRNSTRAIIWHLWRERNSRIFADKSLPFNIFCDIVQLSASNWS 480
            +     G G   +  VLW+ +  AI+W +WRERN+RIF DKS       D++   AS W 
Sbjct: 946  ISINFNGFGSSKRGIVLWQAACIAILWVVWRERNARIFEDKSRNSENLWDMIHFLASLWV 1005

Query: 481  ALDKVFSNYSVAMIHL 489
            +  KVF    + +IHL
Sbjct: 1006 SCSKVFKGIPLNVIHL 1017

BLAST of Spg025874 vs. ExPASy TrEMBL
Match: A5BPI6 (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_008538 PE=4 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 4.0e-98
Identity = 191/496 (38.51%), Postives = 276/496 (55.65%), Query Frame = 0

Query: 1    MQKTGIVGINMEEGEVVRMADLLGCKAEGLPFNYWGVTVDGRPRVASFWDPVLEAIRAKL 60
            + K+ I GIN+ +  + R+A+LL CKA G P  Y G+ + G P+  SFWDPV+E I ++L
Sbjct: 832  LDKSNIYGINLGQDHLHRLAELLDCKASGWPILYLGLPLGGNPKSGSFWDPVIERISSRL 891

Query: 61   AIWKGFPISKGGRTTLIKSVLSSMPVYFFSIFRIPVGVAKEIEKLFRRFLWKG-GDGIRG 120
              W+   +S GGR TLI+S L+ MP YF S+F+IP  VA  IE+L R FLW G G+G R 
Sbjct: 892  DGWQKAYLSFGGRITLIQSCLTHMPCYFLSLFKIPASVAGRIERLQRDFLWSGVGEGKR- 951

Query: 121  SHLVRWDFVTTPLSLRGLGIDDLRAKNAALLAKWLWRFETEPDAMWRRVILSKYG---NN 180
             HLV W+ V       GLG+  +  +N+ALL KWLWR+  E  A+W +VILS YG   N 
Sbjct: 952  DHLVSWBVVCKSKMKGGLGLGRISLRNSALLGKWLWRYPREGSALWHQVILSIYGSHSNG 1011

Query: 181  FIRNHLASKRWKSSSSPWRDIASLAGFFKNHIRYKVGRGDKVMFWEDIWLSDRPLKDRFP 240
            +  N     RW S   PW+ IA +   F    R+ VG GD++ FWED+W  D+ L  RFP
Sbjct: 1012 WDAN--TXVRW-SHRCPWKAIAQVFQDFSKFTRFIVGDGDRIRFWEDLWWGDQSLGVRFP 1071

Query: 241  CIYRLSMAKKAKVCELWNGS---TWNLNMRRGIFDRESSSWIEMVSLLSHFNPNGN-EDL 300
             + R+ M K   +  +   +   +WN N RR + D E      ++  L H + + +  D 
Sbjct: 1072 RLLRVVMDKNILISSILGSTRPFSWNFNFRRNLSDSEIEKVESLMQSLDHIHLSPSVPDK 1131

Query: 301  VQWDLEASGLFSIKSALSVLQQRRRLLEEDLCTKIWEGPIPKRVKFFLWTLALNGINTMD 360
              W L +SGLF++KS    L Q   L        +W   +P ++KFF+W +A   +NT D
Sbjct: 1132 RSWSLSSSGLFTVKSFFLALSQISGLPSVFPTKLVWNSQVPFKIKFFVWLVAHKKVNTND 1191

Query: 361  KVQSRFPSLQISPNVCVMCMKQEESASHLLLHCEFAAKVWNKFEEIFGIQTCNPFSVVSW 420
             +Q R P   +SP++C++CM++ E+  HL LHC     +W++  ++  I    P SV   
Sbjct: 1192 MLQLRRPYKALSPDICMLCMERGETVDHLFLHCSMTMGLWHRLFQLTKIDWVPPRSVFDM 1251

Query: 421  LKEALEGGGLKGKARVLWRNSTRAIIWHLWRERNSRIFADKSLPFNIFCDIVQLSASNWS 480
            +     G G   +  VLW+ +  AI+W +WRERN+RIF DKS       D++   AS W 
Sbjct: 1252 ISINFNGFGSSKRGIVLWQAACIAILWVVWRERNARIFEDKSRNSENLWDMIHFLASLWV 1311

Query: 481  ALDKVFSNYSVAMIHL 489
            +  KVF    + +IHL
Sbjct: 1312 SCSKVFKGIPLNVIHL 1323

BLAST of Spg025874 vs. ExPASy TrEMBL
Match: A5C377 (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_016641 PE=4 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 6.9e-98
Identity = 188/502 (37.45%), Postives = 276/502 (54.98%), Query Frame = 0

Query: 1    MQKTGIVGINMEEGEVVRMADLLGCKAEGLPFNYWGVTVDGRPRVASFWDPVLEAIRAKL 60
            + K+ I GIN+E+  + R+A +L CKA G P  Y G+ + G P+ + FWDPV+E I  +L
Sbjct: 1368 LDKSNIYGINIEQNHLSRLAVMLDCKASGWPILYLGLPLGGNPKASGFWDPVIERISRRL 1427

Query: 61   AIWKGFPISKGGRTTLIKSVLSSMPVYFFSIFRIPVGVAKEIEKLFRRFLWKG-GDGIRG 120
              W+   +S GGR TLI+S L+ MP YF S+FRIP  VA +IE++ R FLW G G+G R 
Sbjct: 1428 DGWQKAYLSFGGRITLIQSCLTHMPCYFLSLFRIPASVAAKIERMQREFLWSGVGEGKR- 1487

Query: 121  SHLVRWDFVTTPLSLRGLGIDDLRAKNAALLAKWLWRFETEPDAMWRRVILSKYGNNF-- 180
             HLV WD V  P S  GLG   +  +N ALL KWLWR+  E  A+W +VILS YG++   
Sbjct: 1488 DHLVNWDVVCKPKSRGGLGFGKISMRNVALLGKWLWRYPREGSALWHQVILSIYGSHSNG 1547

Query: 181  --IRNHLASKRWKSSSSPWRDIASLAGFFKNHIRYKVGRGDKVMFWEDIWLSDRPLKDRF 240
              + N++   RW S   PW+ IA +   F    R+ VG GD++ FW+D+W  D+PL  ++
Sbjct: 1548 WDVNNNV---RW-SHRCPWKAIALVFQEFSKFTRFVVGDGDRIRFWDDLWWGDQPLGTQY 1607

Query: 241  PCIYRLSMAKKAKVCELWNGS---TWNLNMRRGIFDRESSSWIEMVSLLS--HFNPNGNE 300
            P +  +   K A +  +   S   +WN N RR + D E      ++  L   H +P+   
Sbjct: 1608 PRLLSVVTDKNALISSIXGYSRPFSWNFNFRRNLTDSEIEDLESLMRSLDRLHISPS-VX 1667

Query: 301  DLVQWDLEASGLFSIKSALSVLQQRRRLLEEDLCTKIWEGPIPKRVKFFLWTLALNGINT 360
            D   W +  SGLF++KS    L Q            +W   +P +VK F+W +A   +NT
Sbjct: 1668 DKRSWSISPSGLFTVKSFFLALSQXXEXPPVFPTKFVWNSQVPFKVKSFVWLVAHKKLNT 1727

Query: 361  MDKVQSRFPSLQISPNVCVMCMKQEESASHLLLHCEFAAKVWNKFEEIFGIQTCNPFSVV 420
             D +Q R P   +SPN+C +CMK  E+  HL LHC     +W++  ++  +   +P S+ 
Sbjct: 1728 NDLLQLRRPHKALSPNICKLCMKHGETVDHLFLHCSLTKGLWHRLFQLAKMDWVSPRSIS 1787

Query: 421  SWLKEALEGGGLKGKARVLWRNSTRAIIWHLWRERNSRIFADKSLPFNIFCDIVQLSASN 480
                    G G   +  VLW+++  A++W +WRERN+RIF DK+       D ++  AS 
Sbjct: 1788 DMFFTNFNGFGSSKRGVVLWQDACIALMWVVWRERNARIFEDKARNSGCLWDSIRFLASL 1847

Query: 481  WSALDKVFSNYSVAMIHLQWKA 493
            W+   KVF    + M+ L W A
Sbjct: 1848 WAFCSKVFKGIPLNMLQLDWLA 1863

BLAST of Spg025874 vs. ExPASy TrEMBL
Match: A0A438EZ36 (LINE-1 retrotransposable element ORF2 protein OS=Vitis vinifera OX=29760 GN=LORF2_24 PE=4 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 9.0e-98
Identity = 188/502 (37.45%), Postives = 276/502 (54.98%), Query Frame = 0

Query: 1    MQKTGIVGINMEEGEVVRMADLLGCKAEGLPFNYWGVTVDGRPRVASFWDPVLEAIRAKL 60
            + K+ I GIN+E+  + R+A +L CKA G P  Y G+ + G P+ + FWDPV+E I  +L
Sbjct: 1200 LDKSNIYGINIEQNHLSRLAVMLDCKASGWPILYLGLPLGGNPKASGFWDPVIERISRRL 1259

Query: 61   AIWKGFPISKGGRTTLIKSVLSSMPVYFFSIFRIPVGVAKEIEKLFRRFLWKG-GDGIRG 120
              W+   +S GGR TLI+S L+ MP YF S+FRIP  VA +IE++ R FLW G G+G R 
Sbjct: 1260 DGWQKAYLSFGGRITLIQSCLTHMPCYFLSLFRIPASVAAKIERMQREFLWSGVGEGKR- 1319

Query: 121  SHLVRWDFVTTPLSLRGLGIDDLRAKNAALLAKWLWRFETEPDAMWRRVILSKYGNNF-- 180
             HLV WD V  P S  GLG   +  +N ALL KWLWR+  E  A+W +VILS YG++   
Sbjct: 1320 DHLVNWDVVCKPKSRGGLGFGKISMRNVALLGKWLWRYPREGSALWHQVILSIYGSHSNG 1379

Query: 181  --IRNHLASKRWKSSSSPWRDIASLAGFFKNHIRYKVGRGDKVMFWEDIWLSDRPLKDRF 240
              + N++   RW S   PW+ IA +   F    R+ VG GD++ FW+D+W  D+PL  ++
Sbjct: 1380 WDVNNNV---RW-SHRCPWKAIALVFQEFSKFTRFVVGDGDRIRFWDDLWWGDQPLGTQY 1439

Query: 241  PCIYRLSMAKKAKVCELWNGS---TWNLNMRRGIFDRESSSWIEMVSLLS--HFNPNGNE 300
            P +  +   K A +  +   S   +WN N RR + D E      ++  L   H +P+   
Sbjct: 1440 PRLLSVVTDKNALISSILGYSRPFSWNFNFRRNLTDSEIEDLESLMRSLDRLHISPS-VP 1499

Query: 301  DLVQWDLEASGLFSIKSALSVLQQRRRLLEEDLCTKIWEGPIPKRVKFFLWTLALNGINT 360
            D   W +  SGLF++KS    L Q            +W   +P +VK F+W +A   +NT
Sbjct: 1500 DKRSWSISPSGLFTVKSFFLALSQHSESPPVFPTKFVWNSQVPFKVKSFVWLVAHKKLNT 1559

Query: 361  MDKVQSRFPSLQISPNVCVMCMKQEESASHLLLHCEFAAKVWNKFEEIFGIQTCNPFSVV 420
             D +Q R P   +SPN+C +CMK  E+  HL LHC     +W++  ++  +   +P S+ 
Sbjct: 1560 NDLLQLRRPHKALSPNICKLCMKHGETVDHLFLHCSLTKGLWHRLFQLAKMDWVSPRSIS 1619

Query: 421  SWLKEALEGGGLKGKARVLWRNSTRAIIWHLWRERNSRIFADKSLPFNIFCDIVQLSASN 480
                    G G   +  VLW+++  A++W +WRERN+RIF DK+       D ++  AS 
Sbjct: 1620 DMFFTNFNGFGSSKRGVVLWQDACIALMWVVWRERNARIFEDKARNSGCLWDSIRFLASL 1679

Query: 481  WSALDKVFSNYSVAMIHLQWKA 493
            W+   KVF    + M+ L W A
Sbjct: 1680 WAFCSKVFKGIPLNMLQLDWLA 1695

BLAST of Spg025874 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 133.7 bits (335), Expect = 4.2e-31
Identity = 97/392 (24.74%), Postives = 163/392 (41.58%), Query Frame = 0

Query: 83  SMPVYFFSIFRIPVGVAKEIEKLFRRFLWKGGDGIRGSHLVRWDFVTTPLSLRGLGIDDL 142
           ++P Y  + F +P  V K+I  +   F W+     +G H   WD ++   +  G+G  D+
Sbjct: 2   ALPTYTMACFLLPKTVCKQIISVLADFWWRNKQEAKGMHWKAWDHLSCYKAEGGIGFKDI 61

Query: 143 RAKNAALLAKWLWRFETEPDAMWRRVILSKYGNNFIRNHLASKRWKSSSSPWRDIASLAG 202
            A N ALL K +WR  + P+++  +V  S+Y +    + L +      S  W+ I +   
Sbjct: 62  EAFNLALLGKQMWRMLSRPESLMAKVFKSRYFHK--SDPLNAPLGSRPSFVWKSIHASQE 121

Query: 203 FFKNHIRYKVGRGDKVMFWEDIWLSDRPLK-----DRFPCIYRLSMAKKAKVCEL--WNG 262
             +   R  VG G+ ++ W   WL  +P        R P     S++   KV +L   +G
Sbjct: 122 ILRQGARAVVGNGEDIIIWRHKWLDSKPASAALRMQRVPPQEYASVSSILKVSDLIDESG 181

Query: 263 STWNLNMRRGIFDRESSSWIEMVSLLSHFNPNGNE--DLVQWDLEASGLFSIKSALSVLQ 322
             W  ++   +F            L+    P G    D   WD  +SG +++KS   VL 
Sbjct: 182 REWRKDVIEMLFPEVER------KLIGELRPGGRRILDSYTWDYTSSGDYTVKSGYWVLT 241

Query: 323 Q---RRRLLEE-------DLCTKIWEGPIPKRVKFFLWTLALNGINTMDKVQSRFPSLQI 382
           Q   +R   +E        +  KIW+     +++ FLW    N +     +  R  S + 
Sbjct: 242 QIINKRSSPQEVSEPSLNPIYQKIWKSQTSPKIQHFLWKCLSNSLPVAGALAYRHLSKE- 301

Query: 383 SPNVCVMCMKQEESASHLLLHCEFAAKVW--NKFEEIFGIQTCNPFSV-VSWLKEALEGG 442
             + C+ C   +E+ +HLL  C FA   W  +      G +  +   V + W+     G 
Sbjct: 302 --SACIRCPSCKETVNHLLFKCTFARLTWAISSIPIPLGGEWADSIYVNLYWVFNLGNGN 361

Query: 443 GLKGKARVL--WRNSTRAIIWHLWRERNSRIF 451
               KA  L  W      ++W LW+ RN  +F
Sbjct: 362 PQWEKASQLVPW------LLWRLWKNRNELVF 376

BLAST of Spg025874 vs. TAIR 10
Match: AT3G24255.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 97.8 bits (242), Expect = 2.5e-20
Identity = 87/368 (23.64%), Postives = 138/368 (37.50%), Query Frame = 0

Query: 30  LPFNYWGVTVDGRPRVASFWDPVLEAIRAKLAIWKGFPISKGGRTTLIKSVLSSMPVYFF 89
           LP  Y G+ +  +    S + P++E IR ++  W    +S  GR  LI SV+ S+  ++ 
Sbjct: 23  LPVRYLGLPLLTKKMTTSDYGPLVEKIRVRIGKWTARHLSFAGRLQLISSVIHSLTNFWM 82

Query: 90  SIFRIPVGVAKEIEKLFRRFLWKGGDGIRGSHLVRWDFVTTPLSLRGLGIDDLRAKNAAL 149
           S FR+P    KEI+ +   FLW G +       V W  V TP    GLGI  L+      
Sbjct: 83  SAFRLPSACIKEIDSICSSFLWSGPELNTKKAKVAWSDVCTPKDEGGLGIRSLK------ 142

Query: 150 LAKWLWRFETEPDAMWRRVILSKYGNNFIRNHLASKRWKSSSSPWRDIASLAGFFKNHIR 209
                   E    + W     S  GN  +            S  W+ I          ++
Sbjct: 143 --------EANKGSFW-----SISGNTTL-----------GSWMWKKILKHRALASGFVK 202

Query: 210 YKVGRGDKVMFWEDIWLSDRPLKD---RFPCIYRLSMAKKAKVCELWNGSTWNLNMRRGI 269
           + +  G    FW D W     L D      CI  + +   A V E    +  N   RR  
Sbjct: 203 HDIHNGSNTSFWFDNWSKIGRLIDVTGHRGCI-DMGITLHASVAE----AVVNHRPRRHR 262

Query: 270 FDRESSSWIEMVSLLSHFNPNGNEDLVQWDLEA---SGLFSIKSALSVLQQRRRLLEEDL 329
            D       ++++ + H      ED V+W          F+ K   +  ++ +  L+ + 
Sbjct: 263 HD-TLLRIEDVIAEVRHQGLTSGEDTVRWKGNGDIFKPCFNTKETWAATREPK--LKVNW 322

Query: 330 CTKIWEGPIPKRVKFFLWTLALNGINTMDKVQSRFPSLQISPNVCVMCMKQEESASHLLL 389
              +W      +     W    N + T D++ S       S   CV+C    E+  HL  
Sbjct: 323 YKGVWFSHATPKYSVLAWIAIKNRLTTGDRMLSWNAGADSS---CVLCHHLVETRDHLFF 349

Query: 390 HCEFAAKV 392
            C ++A+V
Sbjct: 383 TCPYSAEV 349

BLAST of Spg025874 vs. TAIR 10
Match: AT1G60720.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 79.0 bits (193), Expect = 1.2e-14
Identity = 65/246 (26.42%), Postives = 106/246 (43.09%), Query Frame = 0

Query: 208 IRYKVGRGDKVMFWEDIWLSDRPLKDRFPCI--YRLSMAKKAKVCELWNGSTWNLNMRRG 267
           ++  +G G    FW D W S  PL           L +   A+V E    + W L + R 
Sbjct: 13  VKCNLGNGRIAHFWHDSWTSLGPLIKVMGDYGSRSLRIPLNARVVEALGVNGWKLPLSR- 72

Query: 268 IFDRESSSWIEMVSLLSHFNPNGNEDLVQWDLEASGLFSIKSALSVLQQRRRLLEEDLCT 327
                + +  + +S ++  +P   ED   W +         SA +    R R  E D   
Sbjct: 73  --SAPAQAIHDHISTITTPSPATIEDSFDWVVGGVVCQGFSSARTWDAIRPRAPELDWAK 132

Query: 328 KIW-EGPIPKRVKFFLWTLALNGINTMDKVQSRFPSLQISPNVCVMCMKQEESASHLLLH 387
            +W +G +PK   F +W   L+ + T  ++ S      I    C +C  + ES  HLL  
Sbjct: 133 AVWFKGAVPKHA-FNMWISQLDRLPTRQRLASWG---HIQSFDCCLCTIETESRDHLLFS 192

Query: 388 CEFAAKVWN-KFEEIFGIQT--CNPFSVVSWLKEALEGGGLKGKARVLWRNSTRAIIWHL 447
           CEFAA+VW   F  +   Q   C+   ++SW++ +           +L + S  AII+++
Sbjct: 193 CEFAAQVWRLAFSRLCPRQRLFCSWAELLSWMRSSSSSA-----PSLLRKVSAHAIIYNI 246

BLAST of Spg025874 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 67.0 bits (162), Expect = 4.8e-11
Identity = 63/296 (21.28%), Postives = 115/296 (38.85%), Query Frame = 0

Query: 182 LASKRWKSSSSPWRDIASLAGFFKNHIRYKVGRGDKVMFWEDIWLSDRPLKDRFPCIYRL 241
           L +K  K  S  W  +       K   R+ +G G  +    D  +   P +     +   
Sbjct: 12  LDAKVRKQQSYGWASLLDGIALLKKGTRHLIGDGQNIRIGLDNIVDSHPPRP----LNTE 71

Query: 242 SMAKKAKVCELW--NGS--TWNLNMRRGIFDRESSSWIEMVSLLSHFNPNGNEDLVQWDL 301
              K+  +  L+   GS   W+ +      D+    +I  + L     P    D + W+ 
Sbjct: 72  ETYKEMTINNLFERKGSYYFWDDSKISQFVDQSDHGFIHRIYLAKSKKP----DKIIWNY 131

Query: 302 EASGLFSIKSALSVLQQRRRLL---------EEDLCTKIWEGPIPKRVKFFLWTLALNGI 361
             +G ++++S   +L                  DL T+IW  PI  ++K FLW      +
Sbjct: 132 NTTGEYTVRSGYWLLTHDPSTNIPAINPPHGSIDLKTRIWNLPIMPKLKHFLWRALSQAL 191

Query: 362 NTMDKVQSRFPSLQISPNVCVMCMKQEESASHLLLHCEFAAKVW--------------NK 421
            T +++ +R   ++I P+ C  C ++ ES +H L  C FA   W              N 
Sbjct: 192 ATTERLTTR--GMRIDPS-CPRCHRENESINHALFTCPFATMAWRLSDSSLIRNQLMSND 251

Query: 422 FEEIFGIQTCNPFSVVSWLKEALEGGGLKGKARVLWRNSTRAIIWHLWRERNSRIF 451
           FEE       N  ++++++++         K   +W      +IW +W+ RN+ +F
Sbjct: 252 FEE-------NISNILNFVQDTTMSD--FHKLLPVW------LIWRIWKARNNVVF 281

BLAST of Spg025874 vs. TAIR 10
Match: AT1G45063.1 (copper ion binding;electron carriers )

HSP 1 Score: 52.0 bits (123), Expect = 1.6e-06
Identity = 25/93 (26.88%), Postives = 47/93 (50.54%), Query Frame = 0

Query: 365 PNVCVMCMKQEESASHLLLHCEFAAKVWNKFEEIFGIQTCNPF-SVVSWLKEALEGGGLK 424
           P++C++C   +E+  H+   C F+ +VW+ F     +     F     WL+         
Sbjct: 281 PSLCLLCNALDETRQHVFFDCPFSHEVWSFFCSNARVTPPRMFKDSARWLRHPCR----D 340

Query: 425 GKARVLWRNSTRAIIWHLWRERNSRIFADKSLP 457
            K   + + + +A ++H+WRERN R+ ++KS P
Sbjct: 341 KKVAFILKLAYQASVYHIWRERNIRLNSNKSFP 369