Spg025848 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg025848
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionNAD(P)-binding Rossmann-fold superfamily protein
Locationscaffold7: 272389 .. 276280 (+)
RNA-Seq ExpressionSpg025848
SyntenySpg025848
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAAACTTCTAATTTAACAAAAAAAATATATATATATATATATAATAACGAGATAATTTTGAATTATCAAAAAAAAAAAAAAAAGATAATTTTGCGAATTAAAAAAAAAAAAGCGAGATAATTTTGCAGCACGAGAAAAAGAGTGTGATAATTCTTTTCACCGTACACAAATTTGTCCCTCTCTAAAGGGGGGTTTAGTAATGGCGGGGCGGGCCCAGGTTTCTCTCTGCTCCCACGTTCCTTCTCCTCATAATTTTATCTATTTCTTCAGTTTCCCACTCCACCTCTCTCCGTTTCCCATCTCTATTTATATTTCCCTCTTCACTTCTCAATTTTCATGCCTCATTCTTCCAGATTCACTCACCTCTTTCTTCCATGGATTCCAAGGAATTCGACCTCTCTTCCCCTTCCTCATCTCCCTTCTCCGACAGGTAATCAATCAATCAACCTCCATCGCCGATCTCTTTTCGTCTGATTGTGATTTTCAATTCACTTTCTTCCGCCGTCGTTTTCTTGCAGATGGTGGTCGCACAACACAGTGGCGGTCGTCACCGGGGCCAACAAGGGGATCGGATTTGCCCTAGTTAGAAAATTGGCGCAATCGGAACTCACTGTGATATTAACCGCTAGAGATGAAGGAAGAGGACTGAAGGCCGTCGAAACGCTGCGAAATGAAGGACTGAACGTTCTGTTCCACCGCCTCGATGTTTCCGATCCCGTCTCCATCAGCGCCTTCGCCGACTGGTTCACCGCCAATTTTCAAGCCCTCGACATTCTTGTAAGTATTTCTGAACTTGATATCTGTCCATCTCTGAATTGAATCTCGCCCTTTGTTTTTTCTTCTTTTCAATCGAATTTCGCACATTAACTCCCTTTCTCTGTGCTCTGCGCCATTCATTCACGCTCACGCCTCCTGTTTCTTCCAGAACTACTTTTTCTTTTTCTGATTTTACAATTTCGACCCTCAACTTTCGTGAAATTAGAATTCCACACAATTTTCTCTGGCATTGTTCTCTTACGTCACGTCATAGATTATAAACCGTTTAGTCGATCGTGTTAGCTATTCTGTTGTGTTGTTGTGTGTAAACAAGACTTGCTTTAACATTAAGGTGGTTTTTATTAATCATAATTTAGGCACTTCTTATAACCATTTTATTTTTAATTTTTAGTCATCTATTTCTTCATTGTTTATCATTTTCTTTTTGTTGCATTATTTATTAATTTTAGACCTGTTCGAAAATCTTAATCAATGTTTGTAAGCAGAAAAAAAAAATATTTTCATTAAAATGTTTATTTTGGTGTTTTAATTTATACTTATTTCCCTTTGTTCTATATTTTGATCTTATATAACTTCACATAGTGGGTATGTGACATTAAATTTGGCAGTGTGTATTTAACATTAAATAATAATAATAATAATGAATGGTACATTGATACCGTTCAATGTAATTAAAGCTACATAGGAACGTGTGATTTAAGATCCACATGAAAGGGAAAATTTCAAAATAGTTCTTTAATGAAAGTTAAAATGAAATAAATGTCATATTTTCCCAAGCCAATTTACCCAGCGTAATAATGGTTGAGATATTTAATAACTTTTTGGGTGAGTTGAAAGATCAAACCCTCCTCCCATATTTGGGATGATGTATTCTAAAAAAGAGTTTAATTTACTAGTTTAGTTAATGAATTTTAAAAGTTATGTCAAATAGGTCTGTGAACTATTTTACAATTTTTAAAATTCAACATAAAATTGACTTTTCAATTGATGAGCAATAGGTTCGTAGGTTTTTAAAAATATTTGATTGATTAGGGATCCATTACACACAAAATAGAAAGTTGAGAGCTCTATTAATGTTAGAGACTTAATTTAGTAACCTATTGAAGCTTTCAAAGTTTATAGACTTATTAGAAACAACGAGACTATCATGATGACATGTTATGCTATAATTCTATATTAAAAAAAAAAAAAAAATCTAACTCATAGTTCTAGCAAAATCTGGAAAAGTAGAAAACAAGAGTAAACGAATAATTTGGGAAATGTTCTCACGTTTTTAATGTAAGTAAAAAAAATTGAAATTGTCATGAGGGACTTCAATAAACTCGTTTGAGTATTTTAACTGTAGTAAGCTAAGGAATCTCTTTTGTTTATTTTCAATGTCAAAATTTTTCTTTGACATACTTATATTCATTTTCATTCGACGCTTATGGTAATGGTAGGTAGGCTACGCTGAATTTATGAAAGCTACAAGTCACTTGGTTGAAAGTCAACAAAGATGACATAAATAGTTTTTTTTTTTTTTTTAATAGACTTTCTTCTTTTTCTGTGTAAGGAAAAAGAAATAAGCAAGGACATTGAATGTTCCCAATGAATTTGGTCGTTTTGGCACGTTCCCATCTTGTCGTGGAATCTCTAATTTTTCATGTAATTTCCACTTCAACTGCCACTGTGTCCACCCTTCTTAAAGACGAACTAATATAAGTCATGCACTTTGACCACTCTGCCACTCTCCACAGCTTTCTACTTAGAAAAGTCTCAATATCAAGACCACGAGGACAATATTTTAAACACGTGGCACAATAATATTCCACTCAGTAATTATAAAAATAACCAGACTTTTCTTCTTTTTTCTCATCAGTTGGAGTCTTATGGACCATATATTATATTGTAGATAGAGCTATTATTTTATAGAACAGCGACAGACAACATTATTACATAAAACATAATACTGAGATTTGGTTTTGAATGCATCCATTGTGATAAAGTGATATTTACTTTTGCAGGTTAATAACGCAGCCGTATCATTCAACGACATAAACGAGAACTCAGTGGAAAACGCAGAAACAGTCATAAAAACCAACTTTTACGGCGCCAAATTACTCACCAAAGCTCTATTGCCTTCCTTCCGCTCTTCCGCTTCCAGAAGCCGAATTCTCAACATTACCTCAAGGCTCGGAACCATCGATGTAAGTCTAAAAAAGTTACATGGGTCTCTTTTATTTCTCATTGAAAAGAATATTTTTGTTCGTGAGTATATATAGTAATTACTTAGATTGATAATAATGAAAAAAACATGCAGAAGGTGAAGAACAAGAAAGTGAAAGAGATACTGGAGAGTGAAGATCTGGCGGAGGAGGACATCGATGGCGTAGTGAATGCATTTCTTGAGGATGTGAAGAGAGGGACATGGAAGAAGCAAGGTTGGCCGGCGCTGTGGACGGACTATGCAGTGTCGAAGCTGGCGCTAAACGCTTACACGAGAGTTTTGGCGAAGCGATACGGTGAGTTTGGGAGTGTGCGTGTGAACAGCTTTTGCCCAGGCTTCACTCAGACGTCAATGACACGTGGGAAAGGGACACACACAGCCGACGCCGCCGCACTTGTCGGAGCTAGGCTGGCGTTGCTGCCGCCTCAGCGTTTACCAACTGGTCAGTTCTTCTCGTGGGGGCCTAATTATGATAGGCCCAGAAATTCCAAATTATAGCCAAATCTCTCTCCGATCCATAATTGATCGAACCAAAAAGTCTAAAATTAGTCTTTTTACCGATTCTTCCTTTGGCGTTATTTCTGCCTGTCCTCTGTGTCAGTTGATATGATTGAATGCAATGCATTTGCAAGCAAGCAATCTCTTTGCTCTCATGTGGCGTTGTGTAATATTATTGTTATTTTGAATTTTTGTCTATTTTGTTTTCACTTTACACGAATTGTTAGTTTAGATGTTCTTCTGAAAAACAAGGAAAAGAGATGGTAGCAGCCTTGATCTGTTTTACTTTTCCACCGCTATGTGGTGGCTTTTACACAGTTAGGGACGGGGTAACGTAGAAAAGAAGATCCCTTTACAAAGTAATGTTTGAGATGTTTATTAACTCTAAGTCCTGATGGCTAATTAACATTA

mRNA sequence

ATGGATTCCAAGGAATTCGACCTCTCTTCCCCTTCCTCATCTCCCTTCTCCGACAGATGGTGGTCGCACAACACAGTGGCGGTCGTCACCGGGGCCAACAAGGGGATCGGATTTGCCCTAGTTAGAAAATTGGCGCAATCGGAACTCACTGTGATATTAACCGCTAGAGATGAAGGAAGAGGACTGAAGGCCGTCGAAACGCTGCGAAATGAAGGACTGAACGTTCTGTTCCACCGCCTCGATGTTTCCGATCCCGTCTCCATCAGCGCCTTCGCCGACTGGTTCACCGCCAATTTTCAAGCCCTCGACATTCTTGTTAATAACGCAGCCGTATCATTCAACGACATAAACGAGAACTCAGTGGAAAACGCAGAAACAGTCATAAAAACCAACTTTTACGGCGCCAAATTACTCACCAAAGCTCTATTGCCTTCCTTCCGCTCTTCCGCTTCCAGAAGCCGAATTCTCAACATTACCTCAAGGCTCGGAACCATCGATAAGGTGAAGAACAAGAAAGTGAAAGAGATACTGGAGAGTGAAGATCTGGCGGAGGAGGACATCGATGGCGTAGTGAATGCATTTCTTGAGGATGTGAAGAGAGGGACATGGAAGAAGCAAGGTTGGCCGGCGCTGTGGACGGACTATGCAGTGTCGAAGCTGGCGCTAAACGCTTACACGAGAGTTTTGGCGAAGCGATACGGTGAGTTTGGGAGTGTGCGTGTGAACAGCTTTTGCCCAGGCTTCACTCAGACGTCAATGACACGTGGGAAAGGGACACACACAGCCGACGCCGCCGCACTTGTCGGAGCTAGGCTGGCGTTGCTGCCGCCTCAGCGTTTACCAACTGGTCAGTTCTTCTCGTGGGGGCCTAATTATGATAGGCCCAGAAATTCCAAATTATAG

Coding sequence (CDS)

ATGGATTCCAAGGAATTCGACCTCTCTTCCCCTTCCTCATCTCCCTTCTCCGACAGATGGTGGTCGCACAACACAGTGGCGGTCGTCACCGGGGCCAACAAGGGGATCGGATTTGCCCTAGTTAGAAAATTGGCGCAATCGGAACTCACTGTGATATTAACCGCTAGAGATGAAGGAAGAGGACTGAAGGCCGTCGAAACGCTGCGAAATGAAGGACTGAACGTTCTGTTCCACCGCCTCGATGTTTCCGATCCCGTCTCCATCAGCGCCTTCGCCGACTGGTTCACCGCCAATTTTCAAGCCCTCGACATTCTTGTTAATAACGCAGCCGTATCATTCAACGACATAAACGAGAACTCAGTGGAAAACGCAGAAACAGTCATAAAAACCAACTTTTACGGCGCCAAATTACTCACCAAAGCTCTATTGCCTTCCTTCCGCTCTTCCGCTTCCAGAAGCCGAATTCTCAACATTACCTCAAGGCTCGGAACCATCGATAAGGTGAAGAACAAGAAAGTGAAAGAGATACTGGAGAGTGAAGATCTGGCGGAGGAGGACATCGATGGCGTAGTGAATGCATTTCTTGAGGATGTGAAGAGAGGGACATGGAAGAAGCAAGGTTGGCCGGCGCTGTGGACGGACTATGCAGTGTCGAAGCTGGCGCTAAACGCTTACACGAGAGTTTTGGCGAAGCGATACGGTGAGTTTGGGAGTGTGCGTGTGAACAGCTTTTGCCCAGGCTTCACTCAGACGTCAATGACACGTGGGAAAGGGACACACACAGCCGACGCCGCCGCACTTGTCGGAGCTAGGCTGGCGTTGCTGCCGCCTCAGCGTTTACCAACTGGTCAGTTCTTCTCGTGGGGGCCTAATTATGATAGGCCCAGAAATTCCAAATTATAG

Protein sequence

MDSKEFDLSSPSSSPFSDRWWSHNTVAVVTGANKGIGFALVRKLAQSELTVILTARDEGRGLKAVETLRNEGLNVLFHRLDVSDPVSISAFADWFTANFQALDILVNNAAVSFNDINENSVENAETVIKTNFYGAKLLTKALLPSFRSSASRSRILNITSRLGTIDKVKNKKVKEILESEDLAEEDIDGVVNAFLEDVKRGTWKKQGWPALWTDYAVSKLALNAYTRVLAKRYGEFGSVRVNSFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFFSWGPNYDRPRNSKL
Homology
BLAST of Spg025848 vs. NCBI nr
Match: XP_038891404.1 ((+)-neomenthol dehydrogenase-like [Benincasa hispida])

HSP 1 Score: 525.8 bits (1353), Expect = 2.5e-145
Identity = 265/300 (88.33%), Postives = 282/300 (94.00%), Query Frame = 0

Query: 1   MDSKEFDLSSPSSSPFSDRWWSHNTVAVVTGANKGIGFALVRKLAQSELTVILTARDEGR 60
           M SKEFDL   SSSPFS+RWWS N+VA+VTGANKGIGFALVRKLAQS+LTVILTARDEGR
Sbjct: 1   MASKEFDL---SSSPFSERWWSKNSVAIVTGANKGIGFALVRKLAQSDLTVILTARDEGR 60

Query: 61  GLKAVETLRNEGLNVLFHRLDVSDPVSISAFADWFTANFQALDILVNNAAVSFNDINENS 120
           GLKAVETLRNEGL+VLF RLDVSDPVSI+AFA WF +NFQALDILVNNAAVSFNDI+ENS
Sbjct: 61  GLKAVETLRNEGLHVLFRRLDVSDPVSINAFAAWFASNFQALDILVNNAAVSFNDIHENS 120

Query: 121 VENAETVIKTNFYGAKLLTKALLPSFRSSASRSRILNITSRLGTIDKVKNKKVKEILESE 180
           VENAETV+KTNFYGAKLLT+ALLP FRSSAS+SRILNITSRLGT+DKV+N KVKEILESE
Sbjct: 121 VENAETVMKTNFYGAKLLTEALLPYFRSSASKSRILNITSRLGTVDKVRNMKVKEILESE 180

Query: 181 DLAEEDIDGVVNAFLEDVKRGTWKKQGWPALWTDYAVSKLALNAYTRVLAKRYGEFGSVR 240
           D+ EEDI+GVVNAFL+DVKRGTWKK GWP LWTDYAVSKLALN YTRVLAKRYGE+GSV 
Sbjct: 181 DVGEEDIEGVVNAFLKDVKRGTWKKAGWPTLWTDYAVSKLALNTYTRVLAKRYGEYGSVS 240

Query: 241 VNSFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFFSWGPNYDRPRNSKL 300
           VNSFCPGFTQTSMTRGKGTHTADAAALVGARLALLPP RLPTGQFF WGPNYD PRNSKL
Sbjct: 241 VNSFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPHRLPTGQFFFWGPNYDIPRNSKL 297

BLAST of Spg025848 vs. NCBI nr
Match: KAG6573598.1 ((+)-neomenthol dehydrogenase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7012680.1 (+)-neomenthol dehydrogenase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 517.3 bits (1331), Expect = 8.8e-143
Identity = 265/300 (88.33%), Postives = 280/300 (93.33%), Query Frame = 0

Query: 1   MDSKEFDLSSPSSSPFSDRWWSHNTVAVVTGANKGIGFALVRKLAQSELTVILTARDEGR 60
           MDSKEFDLSS SSSPFS+RWWS+NTVAVVTGANKGIGFALVR+LAQ ELTVILT R+EGR
Sbjct: 1   MDSKEFDLSSASSSPFSNRWWSNNTVAVVTGANKGIGFALVRRLAQMELTVILTTRNEGR 60

Query: 61  GLKAVETLRNEGLNVLFHRLDVSDPVSISAFADWFTANFQALDILVNNAAVSFNDINENS 120
           GLKAVE+LRNEGLNVLF RLDVSDP SI+AFA WF ANF ALDILVNNAAVSFNDI+ NS
Sbjct: 61  GLKAVESLRNEGLNVLFRRLDVSDPASINAFAAWFVANFPALDILVNNAAVSFNDIDHNS 120

Query: 121 VENAETVIKTNFYGAKLLTKALLPSFRSSASRSRILNITSRLGTIDKVKNKKVKEILESE 180
           VENAETVIKTNFYGAKLLT+ALLP FRSS+SRSRILNITSRLGTIDKVKN+KVKEIL+SE
Sbjct: 121 VENAETVIKTNFYGAKLLTQALLPLFRSSSSRSRILNITSRLGTIDKVKNEKVKEILQSE 180

Query: 181 DLAEEDIDGVVNAFLEDVKRGTWKKQGWPALWTDYAVSKLALNAYTRVLAKRYGEFGSVR 240
           DLAEEDIDGVV+AFL+DVKRGTWKK+GWPALWTDYAVSKLALNAYTRVLAKR     SV 
Sbjct: 181 DLAEEDIDGVVDAFLQDVKRGTWKKKGWPALWTDYAVSKLALNAYTRVLAKR---SRSVS 240

Query: 241 VNSFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFFSWGPNYDRPRNSKL 300
           VNSFCPGFTQTSMT GKGTHTADAAA+VGARLALLPP RLPTGQFF WGPNYD PRNSKL
Sbjct: 241 VNSFCPGFTQTSMTGGKGTHTADAAAVVGARLALLPPHRLPTGQFFYWGPNYDTPRNSKL 297

BLAST of Spg025848 vs. NCBI nr
Match: XP_022945555.1 ((+)-neomenthol dehydrogenase-like [Cucurbita moschata])

HSP 1 Score: 517.3 bits (1331), Expect = 8.8e-143
Identity = 265/300 (88.33%), Postives = 280/300 (93.33%), Query Frame = 0

Query: 1   MDSKEFDLSSPSSSPFSDRWWSHNTVAVVTGANKGIGFALVRKLAQSELTVILTARDEGR 60
           MDSKEFDLSS SSSPFSDRWWS+NTVAVVTGANKGIGFALVR+LAQ ELTVILT R+EGR
Sbjct: 1   MDSKEFDLSSASSSPFSDRWWSNNTVAVVTGANKGIGFALVRRLAQMELTVILTTRNEGR 60

Query: 61  GLKAVETLRNEGLNVLFHRLDVSDPVSISAFADWFTANFQALDILVNNAAVSFNDINENS 120
           GLKAVE+LRNEGLNVLF RLDVSDP SI+AFA W  ANF ALDILVNNAAVSFNDI+ NS
Sbjct: 61  GLKAVESLRNEGLNVLFRRLDVSDPASINAFAAWVVANFPALDILVNNAAVSFNDIDHNS 120

Query: 121 VENAETVIKTNFYGAKLLTKALLPSFRSSASRSRILNITSRLGTIDKVKNKKVKEILESE 180
           VENAETVIKTNFYGAKLLT+ALLP FRSS+SRSRILNITSRLGTIDKVKN+KVKEIL+SE
Sbjct: 121 VENAETVIKTNFYGAKLLTQALLPLFRSSSSRSRILNITSRLGTIDKVKNEKVKEILQSE 180

Query: 181 DLAEEDIDGVVNAFLEDVKRGTWKKQGWPALWTDYAVSKLALNAYTRVLAKRYGEFGSVR 240
           DLAEEDI+GVV+AFL+DVKRGTWKK+GWPALWTDYAVSKLALNAYTRVLAKR     SV 
Sbjct: 181 DLAEEDIEGVVDAFLQDVKRGTWKKKGWPALWTDYAVSKLALNAYTRVLAKR---SRSVS 240

Query: 241 VNSFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFFSWGPNYDRPRNSKL 300
           VNSFCPGFTQTSMT GKGTHTADAAA+VGARLALLPP RLPTGQFFSWGPNYD PRNSKL
Sbjct: 241 VNSFCPGFTQTSMTGGKGTHTADAAAVVGARLALLPPHRLPTGQFFSWGPNYDTPRNSKL 297

BLAST of Spg025848 vs. NCBI nr
Match: XP_022966808.1 ((+)-neomenthol dehydrogenase-like [Cucurbita maxima])

HSP 1 Score: 514.6 bits (1324), Expect = 5.7e-142
Identity = 266/301 (88.37%), Postives = 280/301 (93.02%), Query Frame = 0

Query: 1   MDSKEFDLSSPSSSPFSDRWWSHNTVAVVTGANKGIGFALVRKLAQSELTVILTARDEGR 60
           MDSKEFDLSS SSSPFSDRWWS+NTVAVVTGANKGIGFALVR+LAQ ELTVILT R+EGR
Sbjct: 1   MDSKEFDLSSASSSPFSDRWWSNNTVAVVTGANKGIGFALVRRLAQMELTVILTTRNEGR 60

Query: 61  GLKAVETLRNEGLNVLFHRLDVSDPVSISAFADWFTANFQALDILVNNAAVSFNDINENS 120
           GLKAVE+LRNEGLNV F RLDVSDP SI+AFA WF ANF ALDILVNNAAVSFNDI+ NS
Sbjct: 61  GLKAVESLRNEGLNVRFRRLDVSDPASINAFAAWFVANFPALDILVNNAAVSFNDIDHNS 120

Query: 121 VENAETVIKTNFYGAKLLTKALLPSFR-SSASRSRILNITSRLGTIDKVKNKKVKEILES 180
           VENAETVIKTNFYGAKLLT+ALLP FR SS+SRSRILNITSRLGTIDKVKN+KVKEIL+S
Sbjct: 121 VENAETVIKTNFYGAKLLTQALLPLFRSSSSSRSRILNITSRLGTIDKVKNEKVKEILQS 180

Query: 181 EDLAEEDIDGVVNAFLEDVKRGTWKKQGWPALWTDYAVSKLALNAYTRVLAKRYGEFGSV 240
           EDLAEEDIDGVV+AFL+DVKRGTWKK+GWPALWTDYAVSKLALNAYTRVLAKR     SV
Sbjct: 181 EDLAEEDIDGVVDAFLQDVKRGTWKKKGWPALWTDYAVSKLALNAYTRVLAKR---SRSV 240

Query: 241 RVNSFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFFSWGPNYDRPRNSK 300
            VNSFCPGFTQTSMT GKGTHTADAAA+VGARLALLPP RLPTGQFFSWGPNYD PRNSK
Sbjct: 241 SVNSFCPGFTQTSMTGGKGTHTADAAAVVGARLALLPPHRLPTGQFFSWGPNYDTPRNSK 298

BLAST of Spg025848 vs. NCBI nr
Match: XP_023542237.1 ((+)-neomenthol dehydrogenase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 513.8 bits (1322), Expect = 9.7e-142
Identity = 266/301 (88.37%), Postives = 280/301 (93.02%), Query Frame = 0

Query: 1   MDSKEFDLSSPSSSPFSDRWWSHNTVAVVTGANKGIGFALVRKLAQSELTVILTARDEGR 60
           MDSKEFDLSS SSSPFSDRWWS+NTVAVVTGANKGIGFALVR+LAQ ELTVILT R+EGR
Sbjct: 1   MDSKEFDLSSASSSPFSDRWWSNNTVAVVTGANKGIGFALVRRLAQMELTVILTTRNEGR 60

Query: 61  GLKAVETLRNEGLNVLFHRLDVSDPVSISAFADWFTANFQALDILVNNAAVSFNDINENS 120
           GLKAVE+LRNEGLNVLF RLDVSDP SI+AFA WF ANF ALDILVNNAAVSFNDI+ NS
Sbjct: 61  GLKAVESLRNEGLNVLFRRLDVSDPASINAFAAWFVANFPALDILVNNAAVSFNDIDHNS 120

Query: 121 VENAETVIKTNFYGAKLLTKALLPSFR-SSASRSRILNITSRLGTIDKVKNKKVKEILES 180
           VENAETVIKTNFYGAKLLT+ALLP FR SS+SRSRILNITSRLGTIDKVKN+KVKEIL+S
Sbjct: 121 VENAETVIKTNFYGAKLLTQALLPLFRSSSSSRSRILNITSRLGTIDKVKNEKVKEILQS 180

Query: 181 EDLAEEDIDGVVNAFLEDVKRGTWKKQGWPALWTDYAVSKLALNAYTRVLAKRYGEFGSV 240
           EDLAEEDIDGVV+AFL+DVKRGTWKK+GWPALWTDYAVSKLALNAYTRVLAKR     SV
Sbjct: 181 EDLAEEDIDGVVDAFLQDVKRGTWKKKGWPALWTDYAVSKLALNAYTRVLAKR---SRSV 240

Query: 241 RVNSFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFFSWGPNYDRPRNSK 300
            VNSFCPGFTQTSMT GKGTHTADAAA+VGARLALLPP RLPTGQFFSWGPNYD PR SK
Sbjct: 241 SVNSFCPGFTQTSMTGGKGTHTADAAAVVGARLALLPPHRLPTGQFFSWGPNYDVPRKSK 298

BLAST of Spg025848 vs. ExPASy Swiss-Prot
Match: Q9M2E2 ((+)-neomenthol dehydrogenase OS=Arabidopsis thaliana OX=3702 GN=SDR1 PE=1 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 3.0e-53
Identity = 125/285 (43.86%), Postives = 172/285 (60.35%), Query Frame = 0

Query: 27  AVVTGANKGIGFALVRKLAQSELTVILTARDEGRGLKAVETLRNE----GLNVLFHRLDV 86
           AVVTGAN+GIGF + R+LA   + V+LT+RDE RGL+AVETL+ E      ++LFH+LDV
Sbjct: 9   AVVTGANRGIGFEICRQLASEGIRVVLTSRDENRGLEAVETLKKELEISDQSLLFHQLDV 68

Query: 87  SDPVSISAFADWFTANFQALDILVNNAAV-------------------SFNDINENSVEN 146
           +DP SI++ A++    F  LDILVNNA +                    +++I   + E 
Sbjct: 69  ADPASITSLAEFVKTQFGKLDILVNNAGIGGIITDAEALRAGAGKEGFKWDEIITETYEL 128

Query: 147 AETVIKTNFYGAKLLTKALLPSFRSSASRSRILNITSRLGTIDKVKNKKVKEIL-ESEDL 206
            E  IK N+YG K + +A +P  + S S  RI+N++S +G +  V N+  K IL ++E+L
Sbjct: 129 TEECIKINYYGPKRMCEAFIPLLKLSDS-PRIVNVSSSMGQLKNVLNEWAKGILSDAENL 188

Query: 207 AEEDIDGVVNAFLEDVKRGTWKKQGWPALWTDYAVSKLALNAYTRVLAKRYGEFGSVRVN 266
            EE ID V+N  L D K GT K++ W    + Y VSK +LN YTRVLAK++ EF   RVN
Sbjct: 189 TEERIDQVINQLLNDFKEGTVKEKNWAKFMSAYVVSKASLNGYTRVLAKKHPEF---RVN 248

Query: 267 SFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFFS 288
           + CPGF +T M    G  + +  A    RLALLP Q  P+G FFS
Sbjct: 249 AVCPGFVKTDMNFKTGVLSVEEGASSPVRLALLPHQETPSGCFFS 289

BLAST of Spg025848 vs. ExPASy Swiss-Prot
Match: Q9ZUH5 (Short-chain dehydrogenase/reductase 2b OS=Arabidopsis thaliana OX=3702 GN=SDR2b PE=1 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 9.1e-50
Identity = 119/284 (41.90%), Postives = 170/284 (59.86%), Query Frame = 0

Query: 27  AVVTGANKGIGFALVRKLAQSELTVILTARDEGRGLKAVETLRNE----GLNVLFHRLDV 86
           A+VTG N+GIGF + R+LA   + VILT+RDE +GL+AVETL+ E      +++FH+LDV
Sbjct: 9   AIVTGGNRGIGFEICRQLANKGIRVILTSRDEKQGLEAVETLKKELEISDQSIVFHQLDV 68

Query: 87  SDPVSISAFADWFTANFQALDILVNNAAV-------------------SFNDINENSVEN 146
           SDPVS+++ A++   +F  LDIL+NNA V                    + +    + E 
Sbjct: 69  SDPVSVTSLAEFVKTHFGKLDILINNAGVGGVITDVDALRAGTGKEGFKWEETITETYEL 128

Query: 147 AETVIKTNFYGAKLLTKALLPSFRSSASRSRILNITSRLGTIDKVKNKKVKEIL-ESEDL 206
           AE  IK N+YG K + +A +P  + S S  RI+N++S +G +  + N+  K IL ++E+L
Sbjct: 129 AEECIKINYYGPKRMCEAFIPLLQLSDS-PRIINVSSFMGQVKNLVNEWAKGILSDAENL 188

Query: 207 AEEDIDGVVNAFLEDVKRGTWKKQGWPALWTDYAVSKLALNAYTRVLAKRYGEFGSVRVN 266
            E  ID V+N  L D+K  T K + W  + + Y VSK  LNAYTR+LAK++ E   +RVN
Sbjct: 189 TEVRIDQVINQLLNDLKEDTAKTKYWAKVMSAYVVSKAGLNAYTRILAKKHPE---IRVN 248

Query: 267 SFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFF 287
           S CPGF +T M    G  + +  A    RLALLP Q  P+G FF
Sbjct: 249 SVCPGFVKTDMNFKTGILSVEEGASSPVRLALLPHQESPSGCFF 288

BLAST of Spg025848 vs. ExPASy Swiss-Prot
Match: A4UHT7 (Salutaridine reductase OS=Papaver bracteatum OX=215227 GN=SALR PE=1 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 8.5e-48
Identity = 125/294 (42.52%), Postives = 168/294 (57.14%), Query Frame = 0

Query: 27  AVVTGANKGIGFALVRKLAQSELTVILTARDEGRGLKAVETLRNEG-LNVLFHRLDVSDP 86
           AVVTG NKGIGF + ++L+ S + V+LT RD  RGL+AVE L+N    NV+FH+LDV+DP
Sbjct: 15  AVVTGGNKGIGFEICKQLSSSGIMVVLTCRDVTRGLEAVEKLKNSNHENVVFHQLDVTDP 74

Query: 87  V-SISAFADWFTANFQALDILVNNAAVS------------FNDINENS------------ 146
           + ++S+ AD+  A F  LDILVNNA V+             +DI E+S            
Sbjct: 75  ITTMSSLADFIKARFGKLDILVNNAGVAGFSVDADRFKAMISDIGEDSEEVVKIYEKPEA 134

Query: 147 -------VENAETVIKTNFYGAKLLTKALLPSFRSSASRSRILNITSRLGTIDKVKNKKV 206
                   E AE  +K N+YG K +T+ LLP  + S S  RI+N++S  G++  V N+  
Sbjct: 135 QELMSETYELAEECLKINYYGVKSVTEVLLPLLQLSDS-PRIVNVSSSTGSLKYVSNETA 194

Query: 207 KEIL-ESEDLAEEDIDGVVNAFLEDVKRGTWKKQGWPALWTDYAVSKLALNAYTRVLAKR 266
            EIL + + L EE ID VVN  L+D K    +  GWP+    Y  SK  LNAYTRVLAK+
Sbjct: 195 LEILGDGDALTEERIDMVVNMLLKDFKENLIETNGWPSFGAAYTTSKACLNAYTRVLAKK 254

Query: 267 YGEFGSVRVNSFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFF 287
             +F   +VN  CPG  +T M  G G +TAD  A    R+AL P    P+G F+
Sbjct: 255 IPKF---QVNCVCPGLVKTEMNYGIGNYTADEGAKHVVRIALFPDDG-PSGFFY 303

BLAST of Spg025848 vs. ExPASy Swiss-Prot
Match: B2X050 ((+)-neomenthol dehydrogenase OS=Capsicum annuum OX=4072 GN=MNR1 PE=1 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 1.5e-44
Identity = 117/301 (38.87%), Postives = 167/301 (55.48%), Query Frame = 0

Query: 27  AVVTGANKGIGFALVRKLAQSELTVILTARDEGRGLKAVETLRNEG----LNVLFHRLDV 86
           AVVTG NKGIG+   R+LA   + V+LT+RDE +G++A+E L+ E      ++LFH+LD+
Sbjct: 11  AVVTGGNKGIGYETCRQLASKGVVVVLTSRDEKKGIEAIERLKEESNFTDEHILFHQLDI 70

Query: 87  SDPVSISAFADWFTANFQALDILVNNAAVS----------FNDINENSV-------ENAE 146
            DP SIS+  +     F  LDIL+NNA +S            +I E  +       EN E
Sbjct: 71  MDPASISSLVNLIKTKFGRLDILINNAGISGVMVEGDVQVLKEILERYISIVFTEDENGE 130

Query: 147 -------------------TVIKTNFYGAKLLTKALLPSFRSSASRSRILNITSRLGTID 206
                                I+TN+YGAK +T+A +P  + S S  RI+N+ S +G + 
Sbjct: 131 EGGWTKSGPGSVTNYELTKECIETNYYGAKRMTEAFIPLLQLSNS-PRIVNVASSMGKLK 190

Query: 207 KVKNKKVKEIL-ESEDLAEEDIDGVVNAFLEDVKRGTWKKQGWPALWTDYAVSKLALNAY 266
            + NK   E+L +++ L EE +D VVN FL+D    + + +GWP+ +T Y VSK +L AY
Sbjct: 191 LLCNKWAIEVLRDADSLTEEKVDQVVNEFLKDFTEKSTESKGWPSYFTAYKVSKASLIAY 250

Query: 267 TRVLAKRYGEFGSVRVNSFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQF 287
           TRVLA +Y  F   R+NS CPG+ +T +    G+ TA   A     LALLP    P+G F
Sbjct: 251 TRVLATKYPNF---RINSVCPGYCKTDVNANTGSLTAGEGAESLVNLALLPNDG-PSGLF 306

BLAST of Spg025848 vs. ExPASy Swiss-Prot
Match: Q6WAU1 ((-)-isopiperitenone reductase OS=Mentha piperita OX=34256 PE=1 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 2.2e-43
Identity = 122/304 (40.13%), Postives = 170/304 (55.92%), Query Frame = 0

Query: 27  AVVTGANKGIGFALVRKLAQSELTVILTARDEGRGLKAVETLRNEGLNV-----LFHRLD 86
           A+VTGANKGIGF + R+LA+  + VILT+R+E RGL+A + L  E LNV     +FH+LD
Sbjct: 8   ALVTGANKGIGFEICRQLAEKGIIVILTSRNEKRGLEARQKLLKE-LNVSENRLVFHQLD 67

Query: 87  VSDPVSISAFADWFTANFQALDILVNNAAVS----------FNDINE------------- 146
           V+D  S++A A +  + F  LDILVNNA VS          FN+  E             
Sbjct: 68  VTDLASVAAVAVFIKSKFGKLDILVNNAGVSGVEMVGDVSVFNEYIEADFKALQALEAGA 127

Query: 147 ---------------NSVENAETVIKTNFYGAKLLTKALLPSFRSSASRSRILNITSRLG 206
                             E A+  + TN+YG K LT+AL+P  + S S  RI+N++S  G
Sbjct: 128 KEEPPFKPKANGEMIEKFEGAKDCVVTNYYGPKRLTQALIPLLQLSPS-PRIVNVSSSFG 187

Query: 207 TIDKVKNKKVKEILESED-LAEEDIDGVVNAFLEDVKRGTWKKQGWPALWTDYAVSKLAL 266
           ++  + N+  K +L  ED L EE +D VV  FL+D+K G  ++  WP  +    VSK AL
Sbjct: 188 SLLLLWNEWAKGVLGDEDRLTEERVDEVVEVFLKDIKEGKLEESQWPPHFAAERVSKAAL 247

Query: 267 NAYTRVLAKRYGEFGSVRVNSFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPT 287
           NAYT++ AK+Y  F   R+N+ CPG+ +T +T   G  +   AA V  +LALLP    P+
Sbjct: 248 NAYTKIAAKKYPSF---RINAICPGYAKTDITFHAGPLSVAEAAQVPVKLALLPDGG-PS 305

BLAST of Spg025848 vs. ExPASy TrEMBL
Match: A0A6J1G196 ((+)-neomenthol dehydrogenase-like OS=Cucurbita moschata OX=3662 GN=LOC111449752 PE=3 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 4.2e-143
Identity = 265/300 (88.33%), Postives = 280/300 (93.33%), Query Frame = 0

Query: 1   MDSKEFDLSSPSSSPFSDRWWSHNTVAVVTGANKGIGFALVRKLAQSELTVILTARDEGR 60
           MDSKEFDLSS SSSPFSDRWWS+NTVAVVTGANKGIGFALVR+LAQ ELTVILT R+EGR
Sbjct: 1   MDSKEFDLSSASSSPFSDRWWSNNTVAVVTGANKGIGFALVRRLAQMELTVILTTRNEGR 60

Query: 61  GLKAVETLRNEGLNVLFHRLDVSDPVSISAFADWFTANFQALDILVNNAAVSFNDINENS 120
           GLKAVE+LRNEGLNVLF RLDVSDP SI+AFA W  ANF ALDILVNNAAVSFNDI+ NS
Sbjct: 61  GLKAVESLRNEGLNVLFRRLDVSDPASINAFAAWVVANFPALDILVNNAAVSFNDIDHNS 120

Query: 121 VENAETVIKTNFYGAKLLTKALLPSFRSSASRSRILNITSRLGTIDKVKNKKVKEILESE 180
           VENAETVIKTNFYGAKLLT+ALLP FRSS+SRSRILNITSRLGTIDKVKN+KVKEIL+SE
Sbjct: 121 VENAETVIKTNFYGAKLLTQALLPLFRSSSSRSRILNITSRLGTIDKVKNEKVKEILQSE 180

Query: 181 DLAEEDIDGVVNAFLEDVKRGTWKKQGWPALWTDYAVSKLALNAYTRVLAKRYGEFGSVR 240
           DLAEEDI+GVV+AFL+DVKRGTWKK+GWPALWTDYAVSKLALNAYTRVLAKR     SV 
Sbjct: 181 DLAEEDIEGVVDAFLQDVKRGTWKKKGWPALWTDYAVSKLALNAYTRVLAKR---SRSVS 240

Query: 241 VNSFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFFSWGPNYDRPRNSKL 300
           VNSFCPGFTQTSMT GKGTHTADAAA+VGARLALLPP RLPTGQFFSWGPNYD PRNSKL
Sbjct: 241 VNSFCPGFTQTSMTGGKGTHTADAAAVVGARLALLPPHRLPTGQFFSWGPNYDTPRNSKL 297

BLAST of Spg025848 vs. ExPASy TrEMBL
Match: A0A6J1HTC2 ((+)-neomenthol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111466403 PE=3 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 2.8e-142
Identity = 266/301 (88.37%), Postives = 280/301 (93.02%), Query Frame = 0

Query: 1   MDSKEFDLSSPSSSPFSDRWWSHNTVAVVTGANKGIGFALVRKLAQSELTVILTARDEGR 60
           MDSKEFDLSS SSSPFSDRWWS+NTVAVVTGANKGIGFALVR+LAQ ELTVILT R+EGR
Sbjct: 1   MDSKEFDLSSASSSPFSDRWWSNNTVAVVTGANKGIGFALVRRLAQMELTVILTTRNEGR 60

Query: 61  GLKAVETLRNEGLNVLFHRLDVSDPVSISAFADWFTANFQALDILVNNAAVSFNDINENS 120
           GLKAVE+LRNEGLNV F RLDVSDP SI+AFA WF ANF ALDILVNNAAVSFNDI+ NS
Sbjct: 61  GLKAVESLRNEGLNVRFRRLDVSDPASINAFAAWFVANFPALDILVNNAAVSFNDIDHNS 120

Query: 121 VENAETVIKTNFYGAKLLTKALLPSFR-SSASRSRILNITSRLGTIDKVKNKKVKEILES 180
           VENAETVIKTNFYGAKLLT+ALLP FR SS+SRSRILNITSRLGTIDKVKN+KVKEIL+S
Sbjct: 121 VENAETVIKTNFYGAKLLTQALLPLFRSSSSSRSRILNITSRLGTIDKVKNEKVKEILQS 180

Query: 181 EDLAEEDIDGVVNAFLEDVKRGTWKKQGWPALWTDYAVSKLALNAYTRVLAKRYGEFGSV 240
           EDLAEEDIDGVV+AFL+DVKRGTWKK+GWPALWTDYAVSKLALNAYTRVLAKR     SV
Sbjct: 181 EDLAEEDIDGVVDAFLQDVKRGTWKKKGWPALWTDYAVSKLALNAYTRVLAKR---SRSV 240

Query: 241 RVNSFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFFSWGPNYDRPRNSK 300
            VNSFCPGFTQTSMT GKGTHTADAAA+VGARLALLPP RLPTGQFFSWGPNYD PRNSK
Sbjct: 241 SVNSFCPGFTQTSMTGGKGTHTADAAAVVGARLALLPPHRLPTGQFFSWGPNYDTPRNSK 298

BLAST of Spg025848 vs. ExPASy TrEMBL
Match: A0A6J1GZE8 ((+)-neomenthol dehydrogenase-like OS=Cucurbita moschata OX=3662 GN=LOC111458888 PE=3 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 1.0e-141
Identity = 260/300 (86.67%), Postives = 277/300 (92.33%), Query Frame = 0

Query: 1   MDSKEFDLSSPSSSPFSDRWWSHNTVAVVTGANKGIGFALVRKLAQSELTVILTARDEGR 60
           M+SKEFDLSS  SSP SDRWWS NTVAVVTGANKGIGFALVR+LAQSELTVILTARDE R
Sbjct: 1   MESKEFDLSSSLSSPVSDRWWSKNTVAVVTGANKGIGFALVRRLAQSELTVILTARDEAR 60

Query: 61  GLKAVETLRNEGLNVLFHRLDVSDPVSISAFADWFTANFQALDILVNNAAVSFNDINENS 120
           GL+AVETLRNEGL+VLF RLDVSDPVSISAFA WFTANFQALDILVNNAAVSFN IN+NS
Sbjct: 61  GLQAVETLRNEGLHVLFRRLDVSDPVSISAFAAWFTANFQALDILVNNAAVSFNQINQNS 120

Query: 121 VENAETVIKTNFYGAKLLTKALLPSFRSSASRSRILNITSRLGTIDKVKNKKVKEILESE 180
           VE+AETV+KTNFYGAKLLT+ALLP FRSS SRSRILNITSRLGTID+V+N KVKEIL+S+
Sbjct: 121 VEHAETVLKTNFYGAKLLTEALLPYFRSSPSRSRILNITSRLGTIDRVRNMKVKEILQSK 180

Query: 181 DLAEEDIDGVVNAFLEDVKRGTWKKQGWPALWTDYAVSKLALNAYTRVLAKRYGEFGSVR 240
           DLAEEDI GVV AFLEDV+RGTW  QGWPA+WTDYAVSKLALN+YT+VLA RYG FGSV 
Sbjct: 181 DLAEEDIHGVVCAFLEDVQRGTWTNQGWPAVWTDYAVSKLALNSYTKVLANRYGAFGSVS 240

Query: 241 VNSFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFFSWGPNYDRPRNSKL 300
           VN FCPGFTQTSMT GKGTHTADAAALVGARLALLPP RLPTGQFFSWGPNY  P+NSKL
Sbjct: 241 VNCFCPGFTQTSMTGGKGTHTADAAALVGARLALLPPHRLPTGQFFSWGPNYVTPKNSKL 300

BLAST of Spg025848 vs. ExPASy TrEMBL
Match: A0A6J1KA32 ((+)-neomenthol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111493032 PE=3 SV=1)

HSP 1 Score: 511.9 bits (1317), Expect = 1.8e-141
Identity = 262/300 (87.33%), Postives = 278/300 (92.67%), Query Frame = 0

Query: 1   MDSKEFDLSSPSSSPFSDRWWSHNTVAVVTGANKGIGFALVRKLAQSELTVILTARDEGR 60
           M+SKEFDLSS SSSP SDRWWS NTVAVVTGANKGIGFALVR+LAQSELTVILTARDE R
Sbjct: 1   MESKEFDLSS-SSSPVSDRWWSKNTVAVVTGANKGIGFALVRRLAQSELTVILTARDEAR 60

Query: 61  GLKAVETLRNEGLNVLFHRLDVSDPVSISAFADWFTANFQALDILVNNAAVSFNDINENS 120
           GL+AVETLRNEGL+VLF RLDVSDPVSISAFA WFTANFQALDILVNNAAVSFNDIN+NS
Sbjct: 61  GLQAVETLRNEGLHVLFRRLDVSDPVSISAFAAWFTANFQALDILVNNAAVSFNDINQNS 120

Query: 121 VENAETVIKTNFYGAKLLTKALLPSFRSSASRSRILNITSRLGTIDKVKNKKVKEILESE 180
           VE+AETV+KTNFYGAKLLT+ALLP F SS SRSRILNITSRLGTIDKV+N KVKEIL+S+
Sbjct: 121 VEHAETVLKTNFYGAKLLTEALLPYFSSSPSRSRILNITSRLGTIDKVRNIKVKEILQSK 180

Query: 181 DLAEEDIDGVVNAFLEDVKRGTWKKQGWPALWTDYAVSKLALNAYTRVLAKRYGEFGSVR 240
           DLAEEDI G+V AFLEDV+RGTW  QGWPA+WTDYAVSKLALN+YTRVLA RYG FGSV 
Sbjct: 181 DLAEEDIHGIVCAFLEDVQRGTWTNQGWPAVWTDYAVSKLALNSYTRVLANRYGAFGSVS 240

Query: 241 VNSFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFFSWGPNYDRPRNSKL 300
           VN FCPGFTQTSMT GKGTHTADAAALVGARLALLPP RLPTGQFFSWGPNY  P+NSKL
Sbjct: 241 VNCFCPGFTQTSMTGGKGTHTADAAALVGARLALLPPHRLPTGQFFSWGPNYVTPKNSKL 299

BLAST of Spg025848 vs. ExPASy TrEMBL
Match: A0A6J1DC35 ((+)-neomenthol dehydrogenase-like OS=Momordica charantia OX=3673 GN=LOC111019666 PE=3 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 4.9e-139
Identity = 256/302 (84.77%), Postives = 279/302 (92.38%), Query Frame = 0

Query: 1   MDSKEFDLSSPSSSPFSDRWWSHNTVAVVTGANKGIGFALVRKLAQSELTVILTARDEGR 60
           +DSKEFDLSSPSSSPFS RWWS NTVAVVTGANKGIGFALVR+LAQ ELTVILTARD+ R
Sbjct: 14  LDSKEFDLSSPSSSPFSHRWWSKNTVAVVTGANKGIGFALVRRLAQLELTVILTARDQER 73

Query: 61  GLKAVETLRNEGLNVLFHRLDVSDPVSISAFADWFTANFQALDILVNNAAVSFNDINENS 120
           G  AV+TLRNEGL VLF RLDVSDP+SI++FA WFTANFQALDILVNNAAVSFNDINENS
Sbjct: 74  GFNAVQTLRNEGLPVLFRRLDVSDPLSIASFAAWFTANFQALDILVNNAAVSFNDINENS 133

Query: 121 VENAETVIKTNFYGAKLLTKALLPSFRSSA-SRSRILNITSRLGTIDKVKNKKVKEILES 180
           V NAETV+KTN+YGAKLLT+ALLP+FRSSA SR+RILNITSRLGTIDKVKN KV+EIL S
Sbjct: 134 VANAETVMKTNYYGAKLLTQALLPAFRSSAPSRARILNITSRLGTIDKVKNVKVREILGS 193

Query: 181 EDLAEEDIDGVVNAFLEDVKRGTWKKQGWPALWTDYAVSKLALNAYTRVLAKRYGEFG-S 240
           ED+ EEDI+GVVNAFL DVKRGTWKK+GWPA+WT+YAVSKLALNAYT VLAKR+ +FG S
Sbjct: 194 EDVGEEDIEGVVNAFLGDVKRGTWKKEGWPAVWTEYAVSKLALNAYTAVLAKRFAQFGTS 253

Query: 241 VRVNSFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFFSWGPNYDRPRNS 300
           V VNSFCPGFTQTSMTRGKGTHTA+AAA VGARLALLPP RLPTG FFSWGPNYD P+NS
Sbjct: 254 VSVNSFCPGFTQTSMTRGKGTHTAEAAAAVGARLALLPPHRLPTGHFFSWGPNYDVPKNS 313

BLAST of Spg025848 vs. TAIR 10
Match: AT3G59710.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 330.9 bits (847), Expect = 1.1e-90
Identity = 167/268 (62.31%), Postives = 207/268 (77.24%), Query Frame = 0

Query: 20  WWSHNTVAVVTGANKGIGFALVRKLAQSELTVILTARDEGRGLKAVETLRNEGL-NVLFH 79
           WWS  T AVVTGANKGIGFA+V++L +  LTV+LTAR+   G +A E+LR  G  NV F 
Sbjct: 24  WWSEETTAVVTGANKGIGFAVVKRLLELGLTVVLTARNAENGSQAAESLRRIGFGNVHFC 83

Query: 80  RLDVSDPVSISAFADWFTANFQALDILVNNAAVSFNDINENSVENAETVIKTNFYGAKLL 139
            LD+SDP SI+AFA WF  N   LDILVNNAAVSFN + EN ++  ET+IKTNFYGAKLL
Sbjct: 84  CLDISDPSSIAAFASWFGRNLGILDILVNNAAVSFNAVGENLIKEPETIIKTNFYGAKLL 143

Query: 140 TKALLPSFRSSASRSRILNITSRLGTIDKVKNKKVKEILESEDLAEEDIDGVVNAFLEDV 199
           T+ALLP FR S S SRILN++SRLGT++K+++  ++ ILESEDL  E ID  +  FL+DV
Sbjct: 144 TEALLPLFRRSVSVSRILNMSSRLGTLNKLRSPSIRRILESEDLTNEQIDATLTQFLQDV 203

Query: 200 KRGTWKKQGWPALWTDYAVSKLALNAYTRVLAKRYGEFGSVRVNSFCPGFTQTSMTRGKG 259
           K GTW+KQGWP  W DYA+SKLALNAY+RVLA+RY +   + VN  CPGFT+TSMT G+G
Sbjct: 204 KSGTWEKQGWPENWPDYAISKLALNAYSRVLARRY-DGKKLSVNCLCPGFTRTSMTGGQG 263

Query: 260 THTADAAALVGARLALLPPQRLPTGQFF 287
           THTAD AA + A+L LLPP++L TG+F+
Sbjct: 264 THTADEAAAIVAKLVLLPPEKLATGKFY 290

BLAST of Spg025848 vs. TAIR 10
Match: AT5G61830.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 249.2 bits (635), Expect = 4.2e-66
Identity = 140/293 (47.78%), Postives = 197/293 (67.24%), Query Frame = 0

Query: 7   DLSSPSSSPFSD--RWWSHNTVAVVTGANKGIGFALVRKLAQSELTVILTARDEGRGLKA 66
           ++S   + P+SD  RWWS   VAVVTG+N+GIGF + R+LA   LTV+LTAR+   GL+A
Sbjct: 17  EISLLRTIPYSDHHRWWSCENVAVVTGSNRGIGFEIARQLAVHGLTVVLTARNVNAGLEA 76

Query: 67  VETLRN--EGLNVLFHRLDVSDPVSISAFADWFTANFQALDILVNNAAVSFNDINENSVE 126
           V++LR+  EGL V FH+LDV+D  SI  F  W    F  LDILVNNA V++N  ++N+VE
Sbjct: 77  VKSLRHQEEGLKVYFHQLDVTDSSSIREFGCWLKQTFGGLDILVNNAGVNYNLGSDNTVE 136

Query: 127 NAETVIKTNFYGAKLLTKALLPSFRSSASRSRILNITSRLGTI----DKVKNKKVKEILE 186
            AETVI TN+ G K +TKA++P  R S   +R++N++SRLG +    +++ N ++++ L 
Sbjct: 137 FAETVISTNYQGTKNMTKAMIPLMRPSPHGARVVNVSSRLGRVNGRRNRLANVELRDQLS 196

Query: 187 SED-LAEEDIDGVVNAFLEDVKRGTWKKQGWPALWTDYAVSKLALNAYTRVLAK---RYG 246
           S D L EE ID  V+ F+  VK GTW+  GWP  +TDY++SKLA+NAYTR++AK   R G
Sbjct: 197 SPDLLTEELIDRTVSKFINQVKDGTWESGGWPQTFTDYSMSKLAVNAYTRLMAKELERRG 256

Query: 247 EFGSVRVNSFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFFS 288
           E   + VNSFCPG+ +T+MT   G    + AA  G  L+L+  +   TG+FF+
Sbjct: 257 EEEKIYVNSFCPGWVKTAMTGYAGNMPPEDAADTGVWLSLVLSEEAVTGKFFA 309

BLAST of Spg025848 vs. TAIR 10
Match: AT5G51030.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 249.2 bits (635), Expect = 4.2e-66
Identity = 135/296 (45.61%), Postives = 196/296 (66.22%), Query Frame = 0

Query: 2   DSKEFDLSSPSSSPFSD--RWWSHNTVAVVTGANKGIGFALVRKLAQSELTVILTARDEG 61
           D +  ++S   + P+SD  RWW+  TVAVVTGAN+GIGF +VR+LA   LTVILT+RDE 
Sbjct: 13  DKRLQEISLLRTIPYSDHQRWWTSETVAVVTGANRGIGFEMVRQLAGHGLTVILTSRDEN 72

Query: 62  RGLKAVETLRNEGLNVLFHRLDVSDPVSISAFADWFTANFQALDILVNNAAVSFNDINEN 121
            G++A + L+  G NV FHRLD+ D  SI  F +W    +  +D+L+NNA V++N  ++N
Sbjct: 73  VGVEAAKILQEGGFNVDFHRLDILDSSSIQEFCEWIKEKYGFIDVLINNAGVNYNVGSDN 132

Query: 122 SVENAETVIKTNFYGAKLLTKALLPSFRSSASRSRILNITSRLGTI----DKVKNKKVK- 181
           SVE +  VI TN+YG K +  A++P  R +   +RI+N+TSRLG +     K++N+ V+ 
Sbjct: 133 SVEFSHMVISTNYYGTKNIINAMIPLMRHACQGARIVNVTSRLGRLKGRHSKLENEDVRA 192

Query: 182 EILESEDLAEEDIDGVVNAFLEDVKRGTWKKQGWPALWTDYAVSKLALNAYTRVLAKRYG 241
           ++++ + L EE +D  V+ FL+ V+ GTW+  GWP  +TDY+VSK+A+NAYTRVLAK   
Sbjct: 193 KLMDVDSLTEEIVDKTVSEFLKQVEEGTWESGGWPHSFTDYSVSKMAVNAYTRVLAKELS 252

Query: 242 ---EFGSVRVNSFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFFS 288
              E   +  N FCPG+ +T+MT   G  +A+  A  G  LALLP Q + TG+FF+
Sbjct: 253 ERPEGEKIYANCFCPGWVKTAMTGYAGNVSAEDGADTGVWLALLPDQAI-TGKFFA 307

BLAST of Spg025848 vs. TAIR 10
Match: AT3G61220.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 210.3 bits (534), Expect = 2.1e-54
Identity = 125/285 (43.86%), Postives = 172/285 (60.35%), Query Frame = 0

Query: 27  AVVTGANKGIGFALVRKLAQSELTVILTARDEGRGLKAVETLRNE----GLNVLFHRLDV 86
           AVVTGAN+GIGF + R+LA   + V+LT+RDE RGL+AVETL+ E      ++LFH+LDV
Sbjct: 9   AVVTGANRGIGFEICRQLASEGIRVVLTSRDENRGLEAVETLKKELEISDQSLLFHQLDV 68

Query: 87  SDPVSISAFADWFTANFQALDILVNNAAV-------------------SFNDINENSVEN 146
           +DP SI++ A++    F  LDILVNNA +                    +++I   + E 
Sbjct: 69  ADPASITSLAEFVKTQFGKLDILVNNAGIGGIITDAEALRAGAGKEGFKWDEIITETYEL 128

Query: 147 AETVIKTNFYGAKLLTKALLPSFRSSASRSRILNITSRLGTIDKVKNKKVKEIL-ESEDL 206
            E  IK N+YG K + +A +P  + S S  RI+N++S +G +  V N+  K IL ++E+L
Sbjct: 129 TEECIKINYYGPKRMCEAFIPLLKLSDS-PRIVNVSSSMGQLKNVLNEWAKGILSDAENL 188

Query: 207 AEEDIDGVVNAFLEDVKRGTWKKQGWPALWTDYAVSKLALNAYTRVLAKRYGEFGSVRVN 266
            EE ID V+N  L D K GT K++ W    + Y VSK +LN YTRVLAK++ EF   RVN
Sbjct: 189 TEERIDQVINQLLNDFKEGTVKEKNWAKFMSAYVVSKASLNGYTRVLAKKHPEF---RVN 248

Query: 267 SFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFFS 288
           + CPGF +T M    G  + +  A    RLALLP Q  P+G FFS
Sbjct: 249 AVCPGFVKTDMNFKTGVLSVEEGASSPVRLALLPHQETPSGCFFS 289

BLAST of Spg025848 vs. TAIR 10
Match: AT3G61220.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 210.3 bits (534), Expect = 2.1e-54
Identity = 125/285 (43.86%), Postives = 172/285 (60.35%), Query Frame = 0

Query: 27  AVVTGANKGIGFALVRKLAQSELTVILTARDEGRGLKAVETLRNE----GLNVLFHRLDV 86
           AVVTGAN+GIGF + R+LA   + V+LT+RDE RGL+AVETL+ E      ++LFH+LDV
Sbjct: 16  AVVTGANRGIGFEICRQLASEGIRVVLTSRDENRGLEAVETLKKELEISDQSLLFHQLDV 75

Query: 87  SDPVSISAFADWFTANFQALDILVNNAAV-------------------SFNDINENSVEN 146
           +DP SI++ A++    F  LDILVNNA +                    +++I   + E 
Sbjct: 76  ADPASITSLAEFVKTQFGKLDILVNNAGIGGIITDAEALRAGAGKEGFKWDEIITETYEL 135

Query: 147 AETVIKTNFYGAKLLTKALLPSFRSSASRSRILNITSRLGTIDKVKNKKVKEIL-ESEDL 206
            E  IK N+YG K + +A +P  + S S  RI+N++S +G +  V N+  K IL ++E+L
Sbjct: 136 TEECIKINYYGPKRMCEAFIPLLKLSDS-PRIVNVSSSMGQLKNVLNEWAKGILSDAENL 195

Query: 207 AEEDIDGVVNAFLEDVKRGTWKKQGWPALWTDYAVSKLALNAYTRVLAKRYGEFGSVRVN 266
            EE ID V+N  L D K GT K++ W    + Y VSK +LN YTRVLAK++ EF   RVN
Sbjct: 196 TEERIDQVINQLLNDFKEGTVKEKNWAKFMSAYVVSKASLNGYTRVLAKKHPEF---RVN 255

Query: 267 SFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFFS 288
           + CPGF +T M    G  + +  A    RLALLP Q  P+G FFS
Sbjct: 256 AVCPGFVKTDMNFKTGVLSVEEGASSPVRLALLPHQETPSGCFFS 296

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038891404.12.5e-14588.33(+)-neomenthol dehydrogenase-like [Benincasa hispida][more]
KAG6573598.18.8e-14388.33(+)-neomenthol dehydrogenase, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
XP_022945555.18.8e-14388.33(+)-neomenthol dehydrogenase-like [Cucurbita moschata][more]
XP_022966808.15.7e-14288.37(+)-neomenthol dehydrogenase-like [Cucurbita maxima][more]
XP_023542237.19.7e-14288.37(+)-neomenthol dehydrogenase-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9M2E23.0e-5343.86(+)-neomenthol dehydrogenase OS=Arabidopsis thaliana OX=3702 GN=SDR1 PE=1 SV=1[more]
Q9ZUH59.1e-5041.90Short-chain dehydrogenase/reductase 2b OS=Arabidopsis thaliana OX=3702 GN=SDR2b ... [more]
A4UHT78.5e-4842.52Salutaridine reductase OS=Papaver bracteatum OX=215227 GN=SALR PE=1 SV=1[more]
B2X0501.5e-4438.87(+)-neomenthol dehydrogenase OS=Capsicum annuum OX=4072 GN=MNR1 PE=1 SV=1[more]
Q6WAU12.2e-4340.13(-)-isopiperitenone reductase OS=Mentha piperita OX=34256 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1G1964.2e-14388.33(+)-neomenthol dehydrogenase-like OS=Cucurbita moschata OX=3662 GN=LOC111449752 ... [more]
A0A6J1HTC22.8e-14288.37(+)-neomenthol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111466403 PE... [more]
A0A6J1GZE81.0e-14186.67(+)-neomenthol dehydrogenase-like OS=Cucurbita moschata OX=3662 GN=LOC111458888 ... [more]
A0A6J1KA321.8e-14187.33(+)-neomenthol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111493032 PE... [more]
A0A6J1DC354.9e-13984.77(+)-neomenthol dehydrogenase-like OS=Momordica charantia OX=3673 GN=LOC111019666... [more]
Match NameE-valueIdentityDescription
AT3G59710.11.1e-9062.31NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G61830.14.2e-6647.78NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G51030.14.2e-6645.61NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G61220.12.1e-5443.86NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G61220.22.1e-5443.86NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 100..111
score: 49.24
coord: 237..254
score: 36.6
coord: 26..43
score: 48.2
coord: 215..234
score: 32.16
coord: 147..163
score: 24.43
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 153..161
score: 41.05
coord: 215..234
score: 32.85
coord: 100..111
score: 45.96
IPR002347Short-chain dehydrogenase/reductase SDRPFAMPF00106adh_shortcoord: 26..164
e-value: 3.9E-29
score: 101.5
NoneNo IPR availablePFAMPF13561adh_short_C2coord: 206..259
e-value: 7.4E-8
score: 32.2
NoneNo IPR availableGENE3D3.40.50.720coord: 21..292
e-value: 9.4E-78
score: 263.2
NoneNo IPR availablePANTHERPTHR43490(+)-NEOMENTHOL DEHYDROGENASEcoord: 20..290
NoneNo IPR availablePANTHERPTHR43490:SF60NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEINcoord: 20..290
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 26..289

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg025848.1Spg025848.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity