Spg025803 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg025803
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionIon channel CASTOR-like isoform X2
Locationscaffold7: 249823 .. 260260 (-)
RNA-Seq ExpressionSpg025803
SyntenySpg025803
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCACTTTTATTTCGAAAAATACCCCAATCATTTTTTGTAATTCCCGTTATGTCCCTTGACTCTGAATCATCTCCTTCCTCGAGCAGGGACTGGTTCTTCCCTCCACAATCCTTCATACACTCTTACCCCGCCAAATCTCAAAAGTACATCCGTCGATTTTCTGAAACTTCACGAATATCGCAGCGCTATACCGATTATCAGAGATATCGGAAGAGTTCGTCGTGTATTTCTGATTCGCACTCGTGTTCAACGACCAGTAATGATGTCAAGTTTGCTCGTACTCGACGGAGATTTGATTTCGATCGCCGCAGTGACCTGTCGCTTAAACGTTCCGAATTGGATTCTCCTTCAAAACAGAAGTTGGAACTGCCGGACGTTTCGAGCTCGGCGAAGAAAGTGTCTGATTCCGCGCGTTTGTTTAGGTCGCTTGATAGTACGCTTAAAGTTCGATGGCATTTTTTAGCTATTGCAGTGTCGGTTTGTTCTCTATCTCTCTCTATCCCTTTCCGTTCAAGTGTCTTAAGTTTTTAATGATTTGAAGTTGACATGCTTGTATCTGTGTAGATTTTGGTCGTGATTTTTGCAACATTGGTACATGAAAATTTATCTTTACAGGAGCAAGTCAACGACTTAGAGGTACTGAGCTTGCGAATTATGCTTTCTGACTCTCATTTTCATGTATCTGTTTACCGTTCTGTTTTATGAATTCAACGAGCATGTGCTACTTTCCACATGCAGACCCGGATTTCTAATCTTTACGTCAAATTACGAGCCTGCAATTTGCTTGACTCTGGAAGTGAAGATGACGTACGTTCACCAGACGAACTTGCTGATGTTGCTTCAGATAAAAGATTAAAAACTTTAGCCTTGATCGCATCTCTTACGCTGTTGTCGGCCCCTGTCATTATCCTCAAGTACATTGACTATATCTCTAAATCAAGATCATTTGACCACAATTTGGAAGAAGTTTCACTCAGTAAGCAGCTTGCGTATAAGGTGGATGTCTTTTTCTCTATTCACCCATATGCTAAGCCACTGGCATTGTTGATAGCAACTCTTCTACTGATTATGCTTGGAGGTTTAGCGCTATTTGGAGTGACAGATGATTGCCTGGTAGATTGTCTTTGGTTATCTTGGACATATGTTGCTGATTCTGGAAATCATGCCAACTCTGAAGGTATTGGTCCAAGGCTAGTTTCAGTTTCCATTAGCTTCGGTGGGATGTTAATATTTGCCATGATGCTTGGCCTGGTGTCCGATTCAATATCTGAAAAGTTCGACTCTCTTAGAAAAGGAAGAAGTGAGGTTGTTGAACAAGATCACACTTTAATCCTTGGATGGAGTGATAAACTGGTAAAAATATAATATTTCTGCTCATCTTATGCTAATGTTTCATTTGCTAAAAATTCTGGGAATAAGCTGCATGATTTCTGCTTTTATAATCATTACTCGATGCAAATTCTGTTTGCTTTAGGCGGTTGTAGCACATTGCTAATTGTTCTTCTTTTCCTTTTTCTTTCTGCAATGCTATTACATGGATTTAGGGATCACTTCTGAATCAGATTGCTATAGCCAATGAGAGCTTGGGAGGAGGAACTGTTGTGGTGATGGCTGAACGAGACAAAGAGGAAATGGAACTTGAAATTGCTAAAATGGAGTTTGATTTTAAGGGAACCTCTGTTATATGCCGAAGTGGAAGCCCATTAATTCTGGCCGACTTGAAGAAGGTACTTCTTTGTAATGCAAAAACTTTGTGGTGCTGCTGTTTTCTCCCATCTGTCAAGAAGTTTTCTCCCTTGGAATTAAATAACTCAAAATAGTTGTAGCCCGTGTACTTGGTCGACTAAATAACAGTTTTCATTTATTAACAAGAAGTTAGAGTTCTTGGCATCTGATAAAATAATTATTAACTGCTTCAATTCCAAGTATGGTTTCTCATCTTTTTCTACTTCCGTTAATTTTTCTGCTTCTCAATAAATTACCTTGATTACTTGATTGAAGAAACTCTGTTTAGAGTCATCAAATGAACCATACTGAGGCAAATAAATTGACTTCTGTCCGGAATTTGTGGATTTTTATTGGGTTTCTACCATTGACTAATTGCCAGTTTGGATTTTAAAAATTTTGTGAGATTTGTGGATGGTTAAAAATACAACAGCGTAAAGGAGCCTCTCCAGGCACATCTTTTTATTGTGATAGCCAGTGCTGCAAGAATAAGCCTCTCTTAAGTCTTAACAAGGGTTGCATCCATACAGGTTCTTGTATAACCAATGTCTGCTTTCCCCCCCTTTTATAAGACCCATACAATGACTTAACCATCCGCGTAAAATATAATTTGGTCTATTAATACCATAAACCTCCAAATGTGCATTTCATTATGTACATTATAAGTTTTGCTAACATCGAATATACAGGTTTCTGTGTCAAAGGCCCGTGCAATAATTGTCATTGCTGAGGATGGAAATGCTGATCAAGTAAGTAGAAATATTGGTATCATTGAGTTTAGATGCTCAATCTTTATTTGATATATGTACTTTGCAGAGTGATGCCCGTGCATTGAGAACTGTTTTAAGTCTAACTGGAGTTAAAGAAGGGCTGAGAGGACACATAGTGGTGGAACTTAGTGATCTTGATAATGAGGTTCTTGTTAAACTTGTTGGTGGAGAGCTTGTTGAAACTGTTGTGGCTCATGATGTGATTGGTCGCTTAATGATTCAGTGTGCTCGCCAGCCAGGACTTGCTCAGGTCCTTTATCTAGTTAAATGAGCTTGTTTTATTTTTTAAGATAGAATGAAATATTATCTCCATGCTTCTCAACATTATTCTCGTTGATCAAACATATGTTCTGCCTTCATATAATGATTCTTTTCTACACCTTCTTGTTATGTTGCGAATCATGTATTGTAGATTTGGGAAGATATCCTAGGTTTTGAAAACTGTGAATTCTACATCAAAAGATGGCCACAATTGGATGGTATGCAATTTGAGGATGTATTGATCAGCTTCCCTGATGCAATTCCTTGTGGAATCAAGGTTGCATCACGAGGTGGAAAAATTGTACTGAATCCTGAGGACTCATATGTGCTGCAAGAAGGTGATGAAGTTCTTGTTATAGCAGAGGATGATGATACTTACGCACCAGCCGCATTACCTACGGTGCTTTCATTTCCCTTGGGCTTTTTTTTTTTCATACTGAGGGCCTTCTTGTTCTGTTACTTTATTGCTTGCCTTTCCTTTTCCTATAGTGGTATTTTATATTCAATTGCATTATTAGAAATTTTACAGTCATGCAAATAGGGGGAGGAATTTGGTTAAGGAACCCAAAAGGCAAAAGAAGGAATTAGATTGATAAAAAAGCATCGCTGAGCTTAGCCTATGAAGGAATTTCCTGGATTTGTTTATCAAATAAACAATGCTAACAAAGGGCAATCTTGAGAGAGAGAATCATCGTTCATGCATATTGCAAGAACAGCATGAAAGGCACAGAAGATTCTACTTTGTGGATGGAGGAGAGAGAAAGAATTCTAATTTAAATAAAAGCTCTGATAGCTTTCTTTGGATGATGCCTATAATGGATATAAGTGACAATGAATTTGATCGTTCAGGTAAGAGAAGCATCATTCATGCATATTGCAAGACCAACAAGAAAGCCACAGAAGATTCTACTTTGTGGATGGAGGAGAGACATTGACGATATGATTGTGGTGAGTACGTTGTATTAAAGCCCTAATTCTTTCTTTTTGGCGTTGAATGCTGCAGGTTTGGAGGGGAAGTCTTCCCAAAGACTTTATTGTCCCAAAATCTACTGAAAAAATCCTGTTGTGTGGTTGGCGACGAGATATGGAGGATATGATTATGGTAATGTTATTAAGGTCTGAGAACATGCTCCCAGTGCTTCTTGGGTGCAGGTCTTAGTGTTCACAGGCATGATCATAGTCATTTTGTTATGTTTTTCACATTTTTCATTTTCATCTGTTGAGAGTAGATTTCATGTTTGTAGTTCCTTCTTTAACAAATCCATGCATGATGCTTCATTTATTGGAGTGTATCCTCATTAGTTTCTACCATTATAGGTTTTGGATGCATTTTTGGCACCGGGTTCAGAGCTTTGGATGTTCAATGATGTTCCTGAGAATGAGAGGGAAAAGAAACTTATTGATGGTGGTCTTGATATCAGCCGATTGGAGAATATATCTTTGGTTGACCGTGAGGGAAATGCTGTAATTCGGCGTCATTTGGAAAGCCTTCCCTTGGAATCATTTGATTCAGTGAGTAGCATAATTTATTTTCTCACCCTGGAAGCCAAGATTAAGAATGCGTAGAAAACTACTTTGTTACTTTAAATGGTTCTCTTTCTTGGATTTTATCTTTTTATGACATAACCTCAATCTGCACAGATATTGATTCTTGCTGATGAATCTGTAGAGGATTCAGCTATTCAAGCTGATTCAAGATCTCTTGCAACCTTGTTGTTAATACGTGATATTCAGGTACTTCTCTCGAGATTTGATGTCAAAATATATTAGTTTATGGTATTAACTGCCCTAGCTATGCCCATGAGAGTAAATTCTTCTTGGTGGTCCAAGCCCATGACTAACGTCACGAGCCAGGTCTATCATGGATTGAACCCATTCTCTCGACCCTTCTATTTTTCGATGGCCCTTATTGACCACTAGGGCAACCTATGATGATTAAGAAAAATCATCTTTTCTAAGTAGTATTCCTCACGTGTCTAGATACATTAATAAAAAAATAATTTAATGATATATAAGCTTTTTACAAACTATTTTTTTAATTAAAAAACTCATGGATGAATTTAATTGAGAATGTTTTATACATTTTAGATAAATTTTAAAATTTTAAAAGATTTAAACTAAATTGAGAATGACCTAAAATAAAAATTATGGACCATTAAATATATTTTTCATTTTCAATAATGCCTCATTATATTTTAGGGTAAAATACCTTTTTGGTCCCTCATGTTTCGGGTTCGTTTCCAATAGGTCCCTAGGTTTCAAAATCGGACAATCAAGTCCCTACTGTTCCGAAAATGTATCTATTGAGTCCCTCTAGGGCTGACGTGGCAATTTAAAATAATAAAATATTGATTTTTAATTAAAATAATAATAAAATAATAATAAAATAATAATATTATTATTATAATAATTAAAATATAATAATAATAATAAGGTTTAAATCCTATTTTGGTCCCTAAACTTTAAAGATTATTCTATTTCGGTCCTTCAACTTTCAAAATGTTCAGTTTAGTCCCTGAACTTTCAAGTTTGTTCCATATTGGTCCCTAAACTTTTAAAATGTATATTTTAGTCCCTAAACTTTCAAAAAATGATTATTTTAATCCTTATTTTTATTTCAATTTTAACAAATTAATGACATAGCTTCGAGTATGTGTATTAGATTGTTAATATGAGCATGATTTTAAGTCAAATAAATTAGAGACAAACATTAGGAAAAGCAAATTTGAAGTATAAAAGTAACATAACAAGGATCATAATGATTATTTTTTAAAAGTATAAGGACTAAAATAGACATTTTGAAAGTTCAGGGACTAGAATAGAACAAATGTGAAAGTTCAAGGACCAAAATGAACGTTTTGAAAGTTTAAGGACTTAAATAGAACAAACTTAAAAGTTCAGGGACTAAAATGAGATTTAAACCTAATAATAAATAAATAAATAAAAATAATTATTATTATTAAAAAATCTTCTCTCTCCTCTTTCCCCACTCCCCCTCTCTTCTTCCCACTTCCCTCCCTTCTTTCTCCCCGCCGCCCCTCATCCCCCACCTCCTCTCTCTTTTTCTCCCCGCCGGCGACCCCAATAGCCCCACAACAACCATCCCGCCGCTGGAAAAATCGCCGCCCCACTTCCTATTTCTTTTCCATGTTTCTAATGGAAAAAAGAAACAACCCACTGCCTCTACTCCTTTTCCATGTTTATTTTACCCCTTTTTGCCTTCTTCTCTGCATCTCCCTCTCCACTACCGGCAAACCCTCTCCGCCGCAGTCGCACGCCGGCACACCTCCTTCGACAACGCCTGTTTCTTCCTTCTCTCCCTATCTCTCTCTCTCTGCTGTAACCACGCTGGGCGCCGGCACGCACGACTGCGCGGCTTTCTTCTACTCGTTGCCGACTGCACACCGCATTCTTCTGTCGGTTGCCAAGGTGCACACTCTGTTTTCTGCAACCCCTCCCTCTGCATGGCTGTAATCTCTTCCAAATAGCACCTCGTCAAATCCTCAGAACATTTGCCCATCAGTCGAATCGAAAATGTTCAGCCCCACTTGTAAATTTAACCAAAAACCTCCACAACCGACTAAACGAAGACATTGAGCGTAAATTTTCTGAGAAGAGGCAAGTGATTCTCACTAATGAGCTTGCACACACCACTCTATTGAATTGTCCTTCTGATTTGGTCGCCTTGAGCTTTTTCCGGCGGCGGGATGGTGGTTGTCGGGCTATTGGGGTCGCCGGCGGGGAGAAAAAGAGAGAGGAGGTGGGGGATGAGGAGCAGCGGGAAGAAGAAGGGAGGGAAGTGGGAAGAAGAGAGGGGAAGTGGGGAAAGAGGAGAGAGAAAATATTTTTTTAATCATTATTATTATTTTTATTTGTTTATTTATTATTATTATTATATTTTAATTATAATAATAATAATTTTATTATTTTATTATTATTATATTATTATTTTAATTAAAAATCAATATTTTATTATTTTAAATTGTCACGTCAGCCCTAGAGTGACTCAATAGATACATTTTCGGAACAGTAGAGACTTGATTGTCCGATTTTGAAACCTAGGGACCTATTGGAAACGAACCCGAAACATGAGGGACCAAAAAGGTATTTTACCCTATATTTTATTATTTAAACTTTAACCTTTTATTATATTTCTTCTAAAAAAACTATAGGGAAAATTGCAAAAACTACCCTTGAACTATAGCAATTGTTGCAATTCCACCTTGAACTTTTAATTGTAAAAATTGACCCTTCCAGCTTTCTTATATCTAAAAATTAGACTCTTCTTCTTCTTTTTGTTAACTATAGTTAAAAAAAATAAGATGAAAGAGAAAAAAGATATATTTTTTTCCTCTCTCTTATTCTATGGGATTAGGGACAAAAATGGTGTAAAAAATTGGACAGAAAATTTGAAAACAAAAGTTGTGGAAATTAAGAGGTAGATCTGGATGCCAGAAAACCCGAATACCCATGAAAACACCAGAAAAATTTGGAACTTGAGGTGATCGAAAAATAAAATCATCAATGAAAAGTAAAAACGTGTTTATTTGAAAGATTGGCTCCGACAAGGCTAATGCCTGAAAATTCCGGAACACAATCACTGCTAGGGAAGACCACGCTCTCATGTAATGACATTGGCTCTGAGTACCGATGAAGACGGAGCGAGTATGTTGAGAGAAGAAGACAGAGCCATGACCATGACGGAGGAAGATGTAATTACAATGCTCGTTGTCACTCTGATGATGAGTATGGACGTCGTCGTCATCAGGGTACGGGTGATGATTCTGATTATTCAGATTACGAGTGAATGGGTATTATGAGTTCTAGGTTTTTTGATTTAATAATCTGAGTATCGGTTTTTTTTATGATTATTGATTGGAACTTGGAAGAATTAGGTGTTCAATGAATCTATTTGTATGTCAAATTCGTCTGGCTTCATGGTTTATTTTGAGGGGAATTTTTTTGTTTCGTATCATTTGATTGCTTCATATTCGGAGTCATTTGGCTAAGAAGTAACACGTAATTTCTTTTTTGTTGGATGAGAATGTTCCAATTGAGTAACAATAATTGAAAAACTGTTGCCAAAGAATTCCATCCAGAAAAATGAAAACAAAAGTGTTAGGGCTCCATCTGTACATTTCCTTTCAAAGATTTTAGAAATTTTTTTTTTCATATAAAGGTGGAAAATACAGCTGTTGTTGCATTGACATATATCCTAGCGCTGCTCATGAAAGAGATGTTTCATGAAGATTTTCAAGTGGGAACCTGGCGTTTGGAAACATTTTGAGTAGCCACCACCAATTATAGAACAAGTGAAGTCTTAGGAATAATGGGTTTTGATTCTTCACATTATTCCTGCAAAGGCTGCACCATGGAGGCATTGAGAAAATGAGGATATACTTATTTGGACACTGAACAAAAAAAAAAGATCCTCACTTTCTTGGTATTACTTGATTTTCTACACCATACAAGAAATGGAAGCGATGGCATCTGGCCCTTTGAGTTAGATAGGATGCCATTGCCAGCACTTTGCGTGGGAAATCTCCAGAGCTCTGGAAGCCTCATAATCTAGAACCAATATCTACGGAGACTACACTTTTAAAAATATATACATATTATTTGCCATAAAATCTTGCGTTTAGAGTCTTATTGCATTTTTTGCTGTGACAAGGGTTTCATCTTTCCATTTGGTCAATTGCACAATCAGATTTGTGGTTTTTGCAGGCTAAGCGTCTTCCGGTCAGAGATGCTAAGATTGTAGGTTACAGAGGAAGTTTCTCGCAAGGCTCATGGATTGGAGAAATGCAGCAGGCTTCTGATAAGTCGGTTATTATAAGTGAAATTCTGGATCCAAGGACTAAAAATCTGCTTTCGATGTCAAAAATCAGTGACTATGTCTTGTCAAATGAACTTGTCAGTATGGCCTTAGCCATGGTTGCTGAGGATCGACAGATAAATGATGTTTTGGAGGAGCTCTTTGCAGAAGAGGTATTTTTCCTTTTCAATCAAAACCGAGATGCATATCATCAATAAGGAAAAATATAATTACTTGTGGACCTACATTGAGAAAACTCTTCAACACCCTGCTTCTTTTTAACATCCCATTCCTTCATAGGCACTGAACATTGTTTTCTCCATTATATCTATTCTAACGTGTAGTTTTAGTTGGTTTCATTTTGATTTTTTTTTCTTCTTTTTTGGGTAATGCGTCTACTTCTCTGGCATAACCTGCCGCTTTTCTATTATTATCAGGGTAATGAATTGCATATAAGGCAAGCTGATCTCTACCTTCGTGAAGGAGAGGAACTGAGTTTCTATGAAGTACTTTTACGAGCTCGACAGCGAAGAGAGGTTGTGATTGGTTACCGTTCAGCTAATGCTGAAAGAGCCGTTATCAACCCTCCAGCCAAGAATGAGCGACGGAAGTGGTCTCTAGGAGATGTTTTTGTAGTGATAGCCGAGAAAGAATGACATACATAATGACATTTTTACATAGCAAGTGATTATCCTTCATCATTTTAGCGGTCTGGTTTTCACCATAGAATTCAGAAATTAGTTCCATCAAGGCTCCAAAAAACCAAATTTATAATAACCTTAATCACATTACTTTCCCTTAGAACTCAAAAAAATAGACTATAATCTATAGAACAGTAGAACACATCCTATCCAAGGTCGTAACCTTAATCACATCACTGACATTAATTGAGAAACGAATAACAATTGTTTGGAGTTGTTAATATAATATGCACTCTTTATTCAGGTGGACTAACATATATCCGAAGGAAGATATGCAGAACATCAAAAAGTTTTGGGTTTCTGAGAAGCTATTCAGGTGGTGGCTGGTCAACAGTAACAGCCTTCCCTTTCTCTCTTAGCTTGGCCATAGCTTCTTTCATGGCTTCCTTCCTCTTTTTGCTAGCTAGTACTTTTGCCTGCACTCCACTCAGATTGGGATCCTTCTTCTCCTGCGGTGGAGGAGGCGGCGGCCTTCTGGCAGCCGCTTCTGCACTCCGGTCAACTACATTCAGTCCAAGCACTGCTCCTGTCAAGATCAAGGCAGCGTTCCTGCAATTTGAACCACATCACATTTGTTAAATTGTTTTGAGCAGATATACATTTTTCGTGGATGTGGCTGATAACTTGAGAGGAGTTAATGTTAGAGAAATTCTCTTATTCTAATGTCATGGTAAAACATGGTCAATCAAATCTAAAAACTTAAATGGATAAGTTACAATGTGTTAAATCTTATTGATATGACTAACAAATATTTGATCCAAACTCGAAGCAAAGAAATGCTTGTACCTGCGGGAGATGAGATGGCTGCGTGAAGGTTCATTGCATGCGTTGCTGCGAGGCTGCAGATTTGGAAGCCTTAGACAGGAACATCGAATGTTGGTGAATCTGGTGGAGCATGAGAGAGCGGCTGTGGCCGATACTGAAGATAATGAATGTGCCATCATTGTTTGTCAATCTCACATTACCATGCCGGAGTTTATGATTTAAGAATCGACAAGGCATCTCTTTTGTGCGAATCATTTCTGATGTTTAAACAAAACAGACTATCAAAAAGGATATCGTGTAGGCTTCTGAAGAGTATGGCTATTGAAATCTATTGGTCAGTAGTGGATAAGGCGCTGTACGTGTGGGCCTCAATTACCCTATTTTTGTTTTGTTTTGTTTTT

mRNA sequence

ATGTCCCTTGACTCTGAATCATCTCCTTCCTCGAGCAGGGACTGGTTCTTCCCTCCACAATCCTTCATACACTCTTACCCCGCCAAATCTCAAAAGTACATCCGTCGATTTTCTGAAACTTCACGAATATCGCAGCGCTATACCGATTATCAGAGATATCGGAAGAGTTCGTCGTGTATTTCTGATTCGCACTCGTGTTCAACGACCAGTAATGATGTCAAGTTTGCTCGTACTCGACGGAGATTTGATTTCGATCGCCGCAGTGACCTGTCGCTTAAACGTTCCGAATTGGATTCTCCTTCAAAACAGAAGTTGGAACTGCCGGACGTTTCGAGCTCGGCGAAGAAAGTGTCTGATTCCGCGCGTTTGTTTAGGTCGCTTGATAGTACGCTTAAAGTTCGATGGCATTTTTTAGCTATTGCAGTGTCGATTTTGGTCGTGATTTTTGCAACATTGGTACATGAAAATTTATCTTTACAGGAGCAAGTCAACGACTTAGAGACCCGGATTTCTAATCTTTACGTCAAATTACGAGCCTGCAATTTGCTTGACTCTGGAAGTGAAGATGACGTACGTTCACCAGACGAACTTGCTGATGTTGCTTCAGATAAAAGATTAAAAACTTTAGCCTTGATCGCATCTCTTACGCTGTTGTCGGCCCCTGTCATTATCCTCAAGTACATTGACTATATCTCTAAATCAAGATCATTTGACCACAATTTGGAAGAAGTTTCACTCAGTAAGCAGCTTGCGTATAAGGTGGATGTCTTTTTCTCTATTCACCCATATGCTAAGCCACTGGCATTGTTGATAGCAACTCTTCTACTGATTATGCTTGGAGGTTTAGCGCTATTTGGAGTGACAGATGATTGCCTGGTAGATTGTCTTTGGTTATCTTGGACATATGTTGCTGATTCTGGAAATCATGCCAACTCTGAAGGTATTGGTCCAAGGCTAGTTTCAGTTTCCATTAGCTTCGGTGGGATGTTAATATTTGCCATGATGCTTGGCCTGGTGTCCGATTCAATATCTGAAAAGTTCGACTCTCTTAGAAAAGGAAGAAGTGAGGTTGTTGAACAAGATCACACTTTAATCCTTGGATGGAGTGATAAACTGGGATCACTTCTGAATCAGATTGCTATAGCCAATGAGAGCTTGGGAGGAGGAACTGTTGTGGTGATGGCTGAACGAGACAAAGAGGAAATGGAACTTGAAATTGCTAAAATGGAGTTTGATTTTAAGGGAACCTCTGTTATATGCCGAAGTGGAAGCCCATTAATTCTGGCCGACTTGAAGAAGGTTTCTGTGTCAAAGGCCCGTGCAATAATTGTCATTGCTGAGGATGGAAATGCTGATCAAAGTGATGCCCGTGCATTGAGAACTGTTTTAAGTCTAACTGGAGTTAAAGAAGGGCTGAGAGGACACATAGTGGTGGAACTTAGTGATCTTGATAATGAGGTTCTTGTTAAACTTGTTGGTGGAGAGCTTGTTGAAACTGTTGTGGCTCATGATGTGATTGGTCGCTTAATGATTCAGTGTGCTCGCCAGCCAGGACTTGCTCAGATTTGGGAAGATATCCTAGGTTTTGAAAACTGTGAATTCTACATCAAAAGATGGCCACAATTGGATGGTATGCAATTTGAGGATGTATTGATCAGCTTCCCTGATGCAATTCCTTGTGGAATCAAGGTTGCATCACGAGGTGGAAAAATTGTACTGAATCCTGAGGACTCATATGTGCTGCAAGAAGGTGATGAAGTTCTTGTTATAGCAGAGGATGATGATACTTACGCACCAGCCGCATTACCTACGGTAAGAGAAGCATCATTCATGCATATTGCAAGACCAACAAGAAAGCCACAGAAGATTCTACTTTGTGGATGGAGGAGAGACATTGACGATATGATTGTGGTTTGGAGGGGAAGTCTTCCCAAAGACTTTATTGTCCCAAAATCTACTGAAAAAATCCTGTTGTGTGGTTGGCGACGAGATATGGAGGATATGATTATGGTTTTGGATGCATTTTTGGCACCGGGTTCAGAGCTTTGGATGTTCAATGATGTTCCTGAGAATGAGAGGGAAAAGAAACTTATTGATGGTGGTCTTGATATCAGCCGATTGGAGAATATATCTTTGGTTGACCGTGAGGGAAATGCTGTAATTCGGCGTCATTTGGAAAGCCTTCCCTTGGAATCATTTGATTCAATATTGATTCTTGCTGATGAATCTGTAGAGGATTCAGCTATTCAAGCTGATTCAAGATCTCTTGCAACCTTGTTGTTAATACGTGATATTCAGGGAAGACCACGCTCTCATGTAATGACATTGGCTCTGAGTACCGATGAAGACGGAGCGAGTATGTTGAGAGAAGAAGACAGAGCCATGACCATGACGGAGGAAGATGTAATTACAATGCTCGTTGTCACTCTGATGATGAGTATGGACGTCGTCGTCATCAGGGCTAAGCGTCTTCCGGTCAGAGATGCTAAGATTGTAGGTTACAGAGGAAGTTTCTCGCAAGGCTCATGGATTGGAGAAATGCAGCAGGCTTCTGATAAGTCGGTTATTATAAGTGAAATTCTGGATCCAAGGACTAAAAATCTGCTTTCGATGTCAAAAATCAGTGACTATGTCTTGTCAAATGAACTTGTCAGTATGGCCTTAGCCATGGTTGCTGAGGATCGACAGATAAATGATGTTTTGGAGGAGCTCTTTGCAGAAGAGGGTAATGAATTGCATATAAGGCAAGCTGATCTCTACCTTCGTGAAGGAGAGGAACTGAGTTTCTATGAAGTACTTTTACGAGCTCGACAGCGAAGAGAGGTTGTGATTGGTTACCGTTCAGCTAATGCTGAAAGAGCCGTTATCAACCCTCCAGCCAAGAATGAGCGACGGAAGTGGTCTCTAGGAGATGTTTTTGTAGTGATAGCCGAGAAAGAATGA

Coding sequence (CDS)

ATGTCCCTTGACTCTGAATCATCTCCTTCCTCGAGCAGGGACTGGTTCTTCCCTCCACAATCCTTCATACACTCTTACCCCGCCAAATCTCAAAAGTACATCCGTCGATTTTCTGAAACTTCACGAATATCGCAGCGCTATACCGATTATCAGAGATATCGGAAGAGTTCGTCGTGTATTTCTGATTCGCACTCGTGTTCAACGACCAGTAATGATGTCAAGTTTGCTCGTACTCGACGGAGATTTGATTTCGATCGCCGCAGTGACCTGTCGCTTAAACGTTCCGAATTGGATTCTCCTTCAAAACAGAAGTTGGAACTGCCGGACGTTTCGAGCTCGGCGAAGAAAGTGTCTGATTCCGCGCGTTTGTTTAGGTCGCTTGATAGTACGCTTAAAGTTCGATGGCATTTTTTAGCTATTGCAGTGTCGATTTTGGTCGTGATTTTTGCAACATTGGTACATGAAAATTTATCTTTACAGGAGCAAGTCAACGACTTAGAGACCCGGATTTCTAATCTTTACGTCAAATTACGAGCCTGCAATTTGCTTGACTCTGGAAGTGAAGATGACGTACGTTCACCAGACGAACTTGCTGATGTTGCTTCAGATAAAAGATTAAAAACTTTAGCCTTGATCGCATCTCTTACGCTGTTGTCGGCCCCTGTCATTATCCTCAAGTACATTGACTATATCTCTAAATCAAGATCATTTGACCACAATTTGGAAGAAGTTTCACTCAGTAAGCAGCTTGCGTATAAGGTGGATGTCTTTTTCTCTATTCACCCATATGCTAAGCCACTGGCATTGTTGATAGCAACTCTTCTACTGATTATGCTTGGAGGTTTAGCGCTATTTGGAGTGACAGATGATTGCCTGGTAGATTGTCTTTGGTTATCTTGGACATATGTTGCTGATTCTGGAAATCATGCCAACTCTGAAGGTATTGGTCCAAGGCTAGTTTCAGTTTCCATTAGCTTCGGTGGGATGTTAATATTTGCCATGATGCTTGGCCTGGTGTCCGATTCAATATCTGAAAAGTTCGACTCTCTTAGAAAAGGAAGAAGTGAGGTTGTTGAACAAGATCACACTTTAATCCTTGGATGGAGTGATAAACTGGGATCACTTCTGAATCAGATTGCTATAGCCAATGAGAGCTTGGGAGGAGGAACTGTTGTGGTGATGGCTGAACGAGACAAAGAGGAAATGGAACTTGAAATTGCTAAAATGGAGTTTGATTTTAAGGGAACCTCTGTTATATGCCGAAGTGGAAGCCCATTAATTCTGGCCGACTTGAAGAAGGTTTCTGTGTCAAAGGCCCGTGCAATAATTGTCATTGCTGAGGATGGAAATGCTGATCAAAGTGATGCCCGTGCATTGAGAACTGTTTTAAGTCTAACTGGAGTTAAAGAAGGGCTGAGAGGACACATAGTGGTGGAACTTAGTGATCTTGATAATGAGGTTCTTGTTAAACTTGTTGGTGGAGAGCTTGTTGAAACTGTTGTGGCTCATGATGTGATTGGTCGCTTAATGATTCAGTGTGCTCGCCAGCCAGGACTTGCTCAGATTTGGGAAGATATCCTAGGTTTTGAAAACTGTGAATTCTACATCAAAAGATGGCCACAATTGGATGGTATGCAATTTGAGGATGTATTGATCAGCTTCCCTGATGCAATTCCTTGTGGAATCAAGGTTGCATCACGAGGTGGAAAAATTGTACTGAATCCTGAGGACTCATATGTGCTGCAAGAAGGTGATGAAGTTCTTGTTATAGCAGAGGATGATGATACTTACGCACCAGCCGCATTACCTACGGTAAGAGAAGCATCATTCATGCATATTGCAAGACCAACAAGAAAGCCACAGAAGATTCTACTTTGTGGATGGAGGAGAGACATTGACGATATGATTGTGGTTTGGAGGGGAAGTCTTCCCAAAGACTTTATTGTCCCAAAATCTACTGAAAAAATCCTGTTGTGTGGTTGGCGACGAGATATGGAGGATATGATTATGGTTTTGGATGCATTTTTGGCACCGGGTTCAGAGCTTTGGATGTTCAATGATGTTCCTGAGAATGAGAGGGAAAAGAAACTTATTGATGGTGGTCTTGATATCAGCCGATTGGAGAATATATCTTTGGTTGACCGTGAGGGAAATGCTGTAATTCGGCGTCATTTGGAAAGCCTTCCCTTGGAATCATTTGATTCAATATTGATTCTTGCTGATGAATCTGTAGAGGATTCAGCTATTCAAGCTGATTCAAGATCTCTTGCAACCTTGTTGTTAATACGTGATATTCAGGGAAGACCACGCTCTCATGTAATGACATTGGCTCTGAGTACCGATGAAGACGGAGCGAGTATGTTGAGAGAAGAAGACAGAGCCATGACCATGACGGAGGAAGATGTAATTACAATGCTCGTTGTCACTCTGATGATGAGTATGGACGTCGTCGTCATCAGGGCTAAGCGTCTTCCGGTCAGAGATGCTAAGATTGTAGGTTACAGAGGAAGTTTCTCGCAAGGCTCATGGATTGGAGAAATGCAGCAGGCTTCTGATAAGTCGGTTATTATAAGTGAAATTCTGGATCCAAGGACTAAAAATCTGCTTTCGATGTCAAAAATCAGTGACTATGTCTTGTCAAATGAACTTGTCAGTATGGCCTTAGCCATGGTTGCTGAGGATCGACAGATAAATGATGTTTTGGAGGAGCTCTTTGCAGAAGAGGGTAATGAATTGCATATAAGGCAAGCTGATCTCTACCTTCGTGAAGGAGAGGAACTGAGTTTCTATGAAGTACTTTTACGAGCTCGACAGCGAAGAGAGGTTGTGATTGGTTACCGTTCAGCTAATGCTGAAAGAGCCGTTATCAACCCTCCAGCCAAGAATGAGCGACGGAAGTGGTCTCTAGGAGATGTTTTTGTAGTGATAGCCGAGAAAGAATGA

Protein sequence

MSLDSESSPSSSRDWFFPPQSFIHSYPAKSQKYIRRFSETSRISQRYTDYQRYRKSSSCISDSHSCSTTSNDVKFARTRRRFDFDRRSDLSLKRSELDSPSKQKLELPDVSSSAKKVSDSARLFRSLDSTLKVRWHFLAIAVSILVVIFATLVHENLSLQEQVNDLETRISNLYVKLRACNLLDSGSEDDVRSPDELADVASDKRLKTLALIASLTLLSAPVIILKYIDYISKSRSFDHNLEEVSLSKQLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDCLVDCLWLSWTYVADSGNHANSEGIGPRLVSVSISFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQDHTLILGWSDKLGSLLNQIAIANESLGGGTVVVMAERDKEEMELEIAKMEFDFKGTSVICRSGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVELSDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWPQLDGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPAALPTVREASFMHIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKDFIVPKSTEKILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLIDGGLDISRLENISLVDREGNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLIRDIQGRPRSHVMTLALSTDEDGASMLREEDRAMTMTEEDVITMLVVTLMMSMDVVVIRAKRLPVRDAKIVGYRGSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRREVVIGYRSANAERAVINPPAKNERRKWSLGDVFVVIAEKE
Homology
BLAST of Spg025803 vs. NCBI nr
Match: KAA0034178.1 (ion channel CASTOR-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 1637.1 bits (4238), Expect = 0.0e+00
Identity = 861/980 (87.86%), Postives = 896/980 (91.43%), Query Frame = 0

Query: 1   MSLDSESSPSSSRDWFFPPQSFIHSYPAKSQKYIRRFSETSRISQRYTDYQRYRKSSSCI 60
           MSLDSESSPSSSRDWFFPPQSF+HS+PAKS  YIRRFS+TSR+S+RYTDY RYRK+SSCI
Sbjct: 1   MSLDSESSPSSSRDWFFPPQSFLHSHPAKSPNYIRRFSDTSRLSRRYTDYHRYRKTSSCI 60

Query: 61  SDSHSCSTTSNDVKFARTRRRFDFDRRSDLSLKRSELDSPSKQKLELPDVSSSAKKVSDS 120
           SDSHSCST +NDVKFAR+RRRF FDRRSDLSLKRSE++  SK KLELPDVSSSAKKVSD+
Sbjct: 61  SDSHSCSTITNDVKFARSRRRFYFDRRSDLSLKRSEVEFSSKPKLELPDVSSSAKKVSDT 120

Query: 121 ARLFRSLDSTLKVRWHFLAIAVSILVVIFATLVHENLSLQEQVNDLETRISNLYVKLRAC 180
           +RL +S DS+LKVRW FLAIA SI VV FAT+VHENLSLQEQVN+LETRISNL +KLRAC
Sbjct: 121 SRLSKSFDSSLKVRWLFLAIAASIFVVSFATIVHENLSLQEQVNNLETRISNLNIKLRAC 180

Query: 181 NLLDSGSEDDVRSPDELADVASDKRLKTLALIASLTLLSAPVIILKYIDYISKSRSFDHN 240
           NL D G+EDDVRSPDELADV +DKRLKTLALIASLTLL AP+IILKYIDYISKSRS DHN
Sbjct: 181 NLFDHGNEDDVRSPDELADVVTDKRLKTLALIASLTLLFAPIIILKYIDYISKSRSLDHN 240

Query: 241 LEEVSLSKQLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDCLVDCLWLSW 300
           LEE+SL+K+LAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDD LVDCLWLSW
Sbjct: 241 LEEISLNKRLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDNLVDCLWLSW 300

Query: 301 TYVADSGNHANSEGIGPRLVSVSISFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ 360
           TYVADSGNHANSEG GPRLVSVS+SFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ
Sbjct: 301 TYVADSGNHANSEGFGPRLVSVSVSFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ 360

Query: 361 DHTLILGWSDKLGSLLNQIAIANESLGGGTVVVMAERDKEEMELEIAKMEFDFKGTSVIC 420
           +HTLILGWSDKLGSLLNQI+IANESLGGG VVVMAERDKEEMEL+IAKMEFDFKGTSVIC
Sbjct: 361 NHTLILGWSDKLGSLLNQISIANESLGGGIVVVMAERDKEEMELDIAKMEFDFKGTSVIC 420

Query: 421 RSGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL 480
           R+GSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL
Sbjct: 421 RTGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL 480

Query: 481 SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540
           SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP
Sbjct: 481 SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540

Query: 541 QLDGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA 600
           QL GMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVL+EGDEVLVIAEDDDTYAPA
Sbjct: 541 QLTGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLEEGDEVLVIAEDDDTYAPA 600

Query: 601 ALPTVREASFMHIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKDFIVPKSTEKILLCG 660
            LPTVREASF+HIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKDFIVPKS E+ILLCG
Sbjct: 601 PLPTVREASFIHIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKDFIVPKSAERILLCG 660

Query: 661 WRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLIDGGLDISRLENISLVDREGNAV 720
           WRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKL+DGGLDISRLENISLVDREGNAV
Sbjct: 661 WRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLVDGGLDISRLENISLVDREGNAV 720

Query: 721 IRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLIRDIQGRPRSHVMTLALST 780
           IRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLIRDIQ              
Sbjct: 721 IRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLIRDIQ-------------- 780

Query: 781 DEDGASMLREEDRAMTMTEEDVITMLVVTLMMSMDVVVIRAKRLPVRDAKIVGYRGSFSQ 840
                                                   AKR+PVRDAK   ++GSFSQ
Sbjct: 781 ----------------------------------------AKRMPVRDAKATAHKGSFSQ 840

Query: 841 GSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDV 900
           GSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDV
Sbjct: 841 GSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDV 900

Query: 901 LEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRREVVIGYRSANAERAVINPPA 960
           LEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRREVVIGYRSANAERAVINPPA
Sbjct: 901 LEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRREVVIGYRSANAERAVINPPA 926

Query: 961 KNERRKWSLGDVFVVIAEKE 981
           KNERRKWSL DVFVVIAEKE
Sbjct: 961 KNERRKWSLRDVFVVIAEKE 926

BLAST of Spg025803 vs. NCBI nr
Match: TYK15741.1 (ion channel CASTOR-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 1628.2 bits (4215), Expect = 0.0e+00
Identity = 861/992 (86.79%), Postives = 896/992 (90.32%), Query Frame = 0

Query: 1   MSLDSESSPSSSRDWFFPPQSFIHSYPAKSQKYIRRFSETSRISQRYTDYQRYRKSSSCI 60
           MSLDSESSPSSSRDWFFPPQSF+HS+PAKS  YIRRFS+TSR+S+RYTDY RYRK+SSCI
Sbjct: 1   MSLDSESSPSSSRDWFFPPQSFLHSHPAKSPNYIRRFSDTSRLSRRYTDYHRYRKTSSCI 60

Query: 61  SDSHSCSTTSNDVKFARTRRRFDFDRRSDLSLKRSELDSPSKQKLELPDVSSSAKKVSDS 120
           SDSHSCST +NDVKFAR+RRRF FDRRSDLSLKRSE++  SK KLELPDVSSSAKKVSD+
Sbjct: 61  SDSHSCSTITNDVKFARSRRRFYFDRRSDLSLKRSEVEFSSKPKLELPDVSSSAKKVSDT 120

Query: 121 ARLFRSLDSTLKVRWHFLAIAVSILVVIFATLVHENLSLQEQVNDLETRISNLYVKLRAC 180
           +RL +S DS+LKVRW FLAIA SI VV FAT+VHENLSLQEQVN+LETRISNL +KLRAC
Sbjct: 121 SRLSKSFDSSLKVRWLFLAIAASIFVVSFATIVHENLSLQEQVNNLETRISNLNIKLRAC 180

Query: 181 NLLDSGSEDDVRSPDELADVASDKRLKTLALIASLTLLSAPVIILKYIDYISKSRSFDHN 240
           NL D G+EDDVRSPDELADV +DKRLKTLALIASLTLL AP+IILKYIDYISKSRS DHN
Sbjct: 181 NLFDHGNEDDVRSPDELADVVTDKRLKTLALIASLTLLFAPIIILKYIDYISKSRSLDHN 240

Query: 241 LEEVSLSKQLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDCLVDCLWLSW 300
           LEE+SL+K+LAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDD LVDCLWLSW
Sbjct: 241 LEEISLNKRLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDNLVDCLWLSW 300

Query: 301 TYVADSGNHANSEGIGPRLVSVSISFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ 360
           TYVADSGNHANSEG GPRLVSVS+SFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ
Sbjct: 301 TYVADSGNHANSEGFGPRLVSVSVSFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ 360

Query: 361 DHTLILGWSDKLGSLLNQIAIANESLGGGTVVVMAERDKEEMELEIAKMEFDFKGTSVIC 420
           +HTLILGWSDKLGSLLNQI+IANESLGGG VVVMAERDKEEMEL+IAKMEFDFKGTSVIC
Sbjct: 361 NHTLILGWSDKLGSLLNQISIANESLGGGIVVVMAERDKEEMELDIAKMEFDFKGTSVIC 420

Query: 421 RSGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL 480
           R+GSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL
Sbjct: 421 RTGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL 480

Query: 481 SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540
           SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP
Sbjct: 481 SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540

Query: 541 QLDGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA 600
           QL GMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVL+EGDEVLVIAEDDDTYAPA
Sbjct: 541 QLTGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLEEGDEVLVIAEDDDTYAPA 600

Query: 601 ALPTVREASFMHIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKDFIVPKSTEKILLCG 660
            LPTVREASF+HIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKDFIVPKS E+ILLCG
Sbjct: 601 PLPTVREASFIHIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKDFIVPKSAERILLCG 660

Query: 661 WRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLIDGGLDISRLENISLVDREGNAV 720
           WRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKL+DGGLDISRLENISLVDREGNAV
Sbjct: 661 WRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLVDGGLDISRLENISLVDREGNAV 720

Query: 721 IRRHLESLPLESFDS------------ILILADESVEDSAIQADSRSLATLLLIRDIQGR 780
           IRRHLESLPLESFDS            ILILADESVEDSAIQADSRSLATLLLIRDIQ  
Sbjct: 721 IRRHLESLPLESFDSVSSVISFHPGSEILILADESVEDSAIQADSRSLATLLLIRDIQ-- 780

Query: 781 PRSHVMTLALSTDEDGASMLREEDRAMTMTEEDVITMLVVTLMMSMDVVVIRAKRLPVRD 840
                                                               AKR+PVRD
Sbjct: 781 ----------------------------------------------------AKRMPVRD 840

Query: 841 AKIVGYRGSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMAL 900
           AK   ++GSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMAL
Sbjct: 841 AKATAHKGSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMAL 900

Query: 901 AMVAEDRQINDVLEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRREVVIGYRS 960
           AMVAEDRQINDVLEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRREVVIGYRS
Sbjct: 901 AMVAEDRQINDVLEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRREVVIGYRS 938

Query: 961 ANAERAVINPPAKNERRKWSLGDVFVVIAEKE 981
           ANAERAVINPPAKNERRKWSL DVFVVIAEKE
Sbjct: 961 ANAERAVINPPAKNERRKWSLRDVFVVIAEKE 938

BLAST of Spg025803 vs. NCBI nr
Match: XP_022956408.1 (ion channel CASTOR-like isoform X1 [Cucurbita moschata] >XP_022956409.1 ion channel CASTOR-like isoform X1 [Cucurbita moschata] >XP_022956410.1 ion channel CASTOR-like isoform X1 [Cucurbita moschata] >XP_022956411.1 ion channel CASTOR-like isoform X1 [Cucurbita moschata] >XP_022956412.1 ion channel CASTOR-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1610.5 bits (4169), Expect = 0.0e+00
Identity = 857/998 (85.87%), Postives = 890/998 (89.18%), Query Frame = 0

Query: 1   MSLDSESSPSSSRDWFFPPQSFIHSYPAKSQKYIRRFSETSRISQRYTDYQRYRKSSSCI 60
           MSLDSESSPSS+RDWFFPPQSF+HSYPAKS  YIRRFSETSRIS+RY D+QRYRKSSSCI
Sbjct: 1   MSLDSESSPSSTRDWFFPPQSFLHSYPAKSPTYIRRFSETSRISRRYADHQRYRKSSSCI 60

Query: 61  SDSHSCSTTSNDVKFARTRRRFDFDRRSDLSLKRSELDSPSKQKLELPDVSSSAKKVSDS 120
           SDSHSCS+TSND KFARTRRR DFDRRSDLSLKRSE +  SK+KLE  DVSSSAKKVSD+
Sbjct: 61  SDSHSCSSTSNDAKFARTRRRIDFDRRSDLSLKRSESEFSSKRKLEQSDVSSSAKKVSDT 120

Query: 121 ARLFRSLDSTLKVRWHFLAIAVSILVVIFATLVHENLSLQEQVNDLETRISNLYVKLRAC 180
           +RLFRS DSTLKVRW FLAIA SI +VIFATL+HEN SLQEQVN LE RISNL +KLRAC
Sbjct: 121 SRLFRSFDSTLKVRWRFLAIAASIFIVIFATLLHENSSLQEQVNGLEARISNLNIKLRAC 180

Query: 181 NLLDSGSEDDVRSPDELADVASDKRLKTLALIASLTLLSAPVIILKYIDYISKSRSFDHN 240
           NL DSGSEDDVRSPDELAD   DKRLKTLALIASLTLL AP++ILKYID  SKSRS +HN
Sbjct: 181 NLFDSGSEDDVRSPDELADF-MDKRLKTLALIASLTLLLAPIVILKYID--SKSRSLEHN 240

Query: 241 LEEVSLSKQLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDCLVDCLWLSW 300
           LEEVSL+KQL YKVDVFFS+HPYAKPLALLIATLLLIMLGGLALFGVTDD LVDCLWLSW
Sbjct: 241 LEEVSLNKQLLYKVDVFFSVHPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSW 300

Query: 301 TYVADSGNHANSEGIGPRLVSVSISFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ 360
           TYVADSGNHANSEGIGPRLVSVS+SFGGMLIFAMMLGLVSDSISE+FDSLRKGRSEVVEQ
Sbjct: 301 TYVADSGNHANSEGIGPRLVSVSVSFGGMLIFAMMLGLVSDSISERFDSLRKGRSEVVEQ 360

Query: 361 DHTLILGWSDKLGSLLNQIAIANESLGGGTVVVMAERDKEEMELEIAKMEFDFKGTSVIC 420
           +HTLILGWSDKLGSLLNQI+IANESLGGGTVVVMAERDKEEMEL+IAKMEFDFKGTSVIC
Sbjct: 361 NHTLILGWSDKLGSLLNQISIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVIC 420

Query: 421 RSGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL 480
           R+GSPLILADLKKVSVSKARAI+VIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL
Sbjct: 421 RTGSPLILADLKKVSVSKARAIVVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL 480

Query: 481 SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540
           SDLDNE+LVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP
Sbjct: 481 SDLDNELLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540

Query: 541 QLDGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA 600
           QL+GMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA
Sbjct: 541 QLNGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA 600

Query: 601 ALPTVREASFMHIARPTRKPQKILLCGWRRDIDDMIV------------------VWRGS 660
           ALPTV E SF+HIARPTRKPQKILLCGWRRDIDDMIV                  VWRGS
Sbjct: 601 ALPTVGETSFIHIARPTRKPQKILLCGWRRDIDDMIVVSAFIKALILSFWHWMLQVWRGS 660

Query: 661 LPKDFIVPKSTEKILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLIDGGL 720
           LPKDFIVPKS E+ILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKL+DGGL
Sbjct: 661 LPKDFIVPKSAERILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLVDGGL 720

Query: 721 DISRLENISLVDREGNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLI 780
           DISRLENISLVDREGNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLI
Sbjct: 721 DISRLENISLVDREGNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLI 780

Query: 781 RDIQGRPRSHVMTLALSTDEDGASMLREEDRAMTMTEEDVITMLVVTLMMSMDVVVIRAK 840
           RDIQ                                                      AK
Sbjct: 781 RDIQ------------------------------------------------------AK 840

Query: 841 RLPVRDAKIVGYRGSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNE 900
           R+PVR AK V YRGS SQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNE
Sbjct: 841 RMPVRGAKTVAYRGSVSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNE 900

Query: 901 LVSMALAMVAEDRQINDVLEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRREV 960
           LVSMALAMVAEDRQINDVLEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRRE+
Sbjct: 901 LVSMALAMVAEDRQINDVLEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRREI 941

Query: 961 VIGYRSANAERAVINPPAKNERRKWSLGDVFVVIAEKE 981
           VIGYRSA+AERAVINPPAK+ERRKW LGDVFVVIAEKE
Sbjct: 961 VIGYRSAHAERAVINPPAKHERRKWHLGDVFVVIAEKE 941

BLAST of Spg025803 vs. NCBI nr
Match: XP_022997206.1 (ion channel CASTOR-like isoform X1 [Cucurbita maxima] >XP_022997214.1 ion channel CASTOR-like isoform X1 [Cucurbita maxima] >XP_022997221.1 ion channel CASTOR-like isoform X1 [Cucurbita maxima] >XP_022997229.1 ion channel CASTOR-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1609.7 bits (4167), Expect = 0.0e+00
Identity = 858/998 (85.97%), Postives = 889/998 (89.08%), Query Frame = 0

Query: 1   MSLDSESSPSSSRDWFFPPQSFIHSYPAKSQKYIRRFSETSRISQRYTDYQRYRKSSSCI 60
           MSLDSESSPSSSRDWFFPPQSF+ SYPAKSQ YIRRFSETSRIS+RY D+QRYRKSSSCI
Sbjct: 1   MSLDSESSPSSSRDWFFPPQSFVQSYPAKSQTYIRRFSETSRISRRYADHQRYRKSSSCI 60

Query: 61  SDSHSCSTTSNDVKFARTRRRFDFDRRSDLSLKRSELDSPSKQKLELPDVSSSAKKVSDS 120
           SDSHSCS+TSND KFARTRRR DFDRRSDLSLKRSEL+  SK+KLE  DVSSSAKKVSD+
Sbjct: 61  SDSHSCSSTSNDAKFARTRRRIDFDRRSDLSLKRSELEFSSKRKLEQSDVSSSAKKVSDT 120

Query: 121 ARLFRSLDSTLKVRWHFLAIAVSILVVIFATLVHENLSLQEQVNDLETRISNLYVKLRAC 180
           +RLFRS DSTLKVRW FLAIA SI +VIFATL+HEN SLQEQVN LE RISNL +KLRAC
Sbjct: 121 SRLFRSFDSTLKVRWRFLAIAASIFIVIFATLLHENSSLQEQVNGLEARISNLNIKLRAC 180

Query: 181 NLLDSGSEDDVRSPDELADVASDKRLKTLALIASLTLLSAPVIILKYIDYISKSRSFDHN 240
           NL DSGSEDDVRSPDELAD   DKRLKTLALI SLTLL AP+IILKYID  SKSRS +HN
Sbjct: 181 NLFDSGSEDDVRSPDELADF-MDKRLKTLALITSLTLLLAPIIILKYID--SKSRSLEHN 240

Query: 241 LEEVSLSKQLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDCLVDCLWLSW 300
           LEE+SL+KQL YKVDVFFS+HPYAKPLALLIATL LIMLGGLALFGVTDD LVDCLWLSW
Sbjct: 241 LEEISLNKQLLYKVDVFFSVHPYAKPLALLIATLQLIMLGGLALFGVTDDSLVDCLWLSW 300

Query: 301 TYVADSGNHANSEGIGPRLVSVSISFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ 360
           TYVADSGNHANSEGIGPRLVSVS+SFGGMLIFAMMLGLVSDSISE+FDSLRKGRSEVVEQ
Sbjct: 301 TYVADSGNHANSEGIGPRLVSVSVSFGGMLIFAMMLGLVSDSISERFDSLRKGRSEVVEQ 360

Query: 361 DHTLILGWSDKLGSLLNQIAIANESLGGGTVVVMAERDKEEMELEIAKMEFDFKGTSVIC 420
           +HTLILGWSDKLGSLLNQI+IANESLGGGTVVVMAERDKEEMEL+IAKMEFDFKGTSVIC
Sbjct: 361 NHTLILGWSDKLGSLLNQISIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVIC 420

Query: 421 RSGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL 480
           RSGSPLILADLKKVSVSKARAI+VIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL
Sbjct: 421 RSGSPLILADLKKVSVSKARAIVVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL 480

Query: 481 SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540
           SDLDNE+LVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP
Sbjct: 481 SDLDNELLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540

Query: 541 QLDGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA 600
           QL+GMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA
Sbjct: 541 QLNGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA 600

Query: 601 ALPTVREASFMHIARPTRKPQKILLCGWRRDIDDMIV------------------VWRGS 660
           ALPTV E SF+HIARPTRKPQKILLCGWRRDIDDMIV                  VWRGS
Sbjct: 601 ALPTVGETSFIHIARPTRKPQKILLCGWRRDIDDMIVVSAFIKALILSFWHWMLQVWRGS 660

Query: 661 LPKDFIVPKSTEKILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLIDGGL 720
           LPKDFIVPKS E+ILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKL+DGGL
Sbjct: 661 LPKDFIVPKSAERILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLVDGGL 720

Query: 721 DISRLENISLVDREGNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLI 780
           DISRLENISLVDREGNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLI
Sbjct: 721 DISRLENISLVDREGNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLI 780

Query: 781 RDIQGRPRSHVMTLALSTDEDGASMLREEDRAMTMTEEDVITMLVVTLMMSMDVVVIRAK 840
           RDIQ                                                      AK
Sbjct: 781 RDIQ------------------------------------------------------AK 840

Query: 841 RLPVRDAKIVGYRGSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNE 900
           R+PVR AK V YRGS SQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNE
Sbjct: 841 RMPVRGAKTVTYRGSVSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNE 900

Query: 901 LVSMALAMVAEDRQINDVLEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRREV 960
           LVSMALAMVAEDRQINDVLEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRRE+
Sbjct: 901 LVSMALAMVAEDRQINDVLEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRREI 941

Query: 961 VIGYRSANAERAVINPPAKNERRKWSLGDVFVVIAEKE 981
           VIGYRSA+AERAVINPPAK+ERRKW LGDVFVVIAEKE
Sbjct: 961 VIGYRSAHAERAVINPPAKHERRKWYLGDVFVVIAEKE 941

BLAST of Spg025803 vs. NCBI nr
Match: KAG6601611.1 (Ion channel CASTOR, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1609.7 bits (4167), Expect = 0.0e+00
Identity = 857/998 (85.87%), Postives = 890/998 (89.18%), Query Frame = 0

Query: 1   MSLDSESSPSSSRDWFFPPQSFIHSYPAKSQKYIRRFSETSRISQRYTDYQRYRKSSSCI 60
           MSLDSESSPSS+RDWFFPPQSF+HSYPAKS  YIRRFSETSRIS+RY D+QRYRKSSSCI
Sbjct: 1   MSLDSESSPSSTRDWFFPPQSFLHSYPAKSPTYIRRFSETSRISRRYADHQRYRKSSSCI 60

Query: 61  SDSHSCSTTSNDVKFARTRRRFDFDRRSDLSLKRSELDSPSKQKLELPDVSSSAKKVSDS 120
           SDSHSCS+TSND KFARTRRR DFDRRSDLSLKRSE +  SK+KLE  DVSSSAKKVSD+
Sbjct: 61  SDSHSCSSTSNDAKFARTRRRIDFDRRSDLSLKRSESEFSSKRKLEQSDVSSSAKKVSDT 120

Query: 121 ARLFRSLDSTLKVRWHFLAIAVSILVVIFATLVHENLSLQEQVNDLETRISNLYVKLRAC 180
           +RLFRS DSTLKVRW FLAIA SI +VIFATL+HEN SLQEQVN LE RISNL +KLRAC
Sbjct: 121 SRLFRSFDSTLKVRWRFLAIAASIFLVIFATLLHENSSLQEQVNGLEARISNLNIKLRAC 180

Query: 181 NLLDSGSEDDVRSPDELADVASDKRLKTLALIASLTLLSAPVIILKYIDYISKSRSFDHN 240
           NL DSGSEDDVRSPDELAD   DKRLKTLALIASLTLL AP++ILKYID  SKSRS +HN
Sbjct: 181 NLFDSGSEDDVRSPDELADF-MDKRLKTLALIASLTLLLAPIVILKYID--SKSRSLEHN 240

Query: 241 LEEVSLSKQLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDCLVDCLWLSW 300
           LEEVSL+KQL YKVDVFFS+HPYAKPLALLIATLLLIMLGGLALFGVTDD LVDCLWLSW
Sbjct: 241 LEEVSLNKQLLYKVDVFFSVHPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSW 300

Query: 301 TYVADSGNHANSEGIGPRLVSVSISFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ 360
           TYVADSGNHANSEGIGPRLVSVS+SFGGMLIFAMMLGLVSDSISE+FDSLRKGRSEVVEQ
Sbjct: 301 TYVADSGNHANSEGIGPRLVSVSVSFGGMLIFAMMLGLVSDSISERFDSLRKGRSEVVEQ 360

Query: 361 DHTLILGWSDKLGSLLNQIAIANESLGGGTVVVMAERDKEEMELEIAKMEFDFKGTSVIC 420
           +HTLILGWSDKLGSLLNQI+IANESLGGGTVVVMAERDKEEMEL+IAKMEFDFKGTSVIC
Sbjct: 361 NHTLILGWSDKLGSLLNQISIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVIC 420

Query: 421 RSGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL 480
           R+GSPLILADLKKVSVSKARAI+VIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL
Sbjct: 421 RTGSPLILADLKKVSVSKARAIVVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL 480

Query: 481 SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540
           SDLDNE+LVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP
Sbjct: 481 SDLDNELLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540

Query: 541 QLDGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA 600
           QL+GMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA
Sbjct: 541 QLNGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA 600

Query: 601 ALPTVREASFMHIARPTRKPQKILLCGWRRDIDDMIV------------------VWRGS 660
           ALPTV E SF+HIARPTRKPQKILLCGWRRDIDDMIV                  VWRGS
Sbjct: 601 ALPTVGETSFIHIARPTRKPQKILLCGWRRDIDDMIVVSAFIKALILSFWHWMLQVWRGS 660

Query: 661 LPKDFIVPKSTEKILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLIDGGL 720
           LPKDFIVPKS E+ILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKL+DGGL
Sbjct: 661 LPKDFIVPKSAERILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLVDGGL 720

Query: 721 DISRLENISLVDREGNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLI 780
           DISRLENISLVDREGNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLI
Sbjct: 721 DISRLENISLVDREGNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLI 780

Query: 781 RDIQGRPRSHVMTLALSTDEDGASMLREEDRAMTMTEEDVITMLVVTLMMSMDVVVIRAK 840
           RDIQ                                                      AK
Sbjct: 781 RDIQ------------------------------------------------------AK 840

Query: 841 RLPVRDAKIVGYRGSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNE 900
           R+PVR AK V YRGS SQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNE
Sbjct: 841 RMPVRGAKTVAYRGSVSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNE 900

Query: 901 LVSMALAMVAEDRQINDVLEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRREV 960
           LVSMALAMVAEDRQINDVLEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRRE+
Sbjct: 901 LVSMALAMVAEDRQINDVLEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRREI 941

Query: 961 VIGYRSANAERAVINPPAKNERRKWSLGDVFVVIAEKE 981
           VIGYRSA+AERAVINPPAK+ERRKW LGDVFVVIAEKE
Sbjct: 961 VIGYRSAHAERAVINPPAKHERRKWHLGDVFVVIAEKE 941

BLAST of Spg025803 vs. ExPASy Swiss-Prot
Match: Q5H8A6 (Ion channel CASTOR OS=Lotus japonicus OX=34305 GN=CASTOR PE=1 SV=1)

HSP 1 Score: 1141.7 bits (2952), Expect = 0.0e+00
Identity = 651/984 (66.16%), Postives = 722/984 (73.37%), Query Frame = 0

Query: 1   MSLDSESSPSSS--RDWFFPPQSFIHSYPAKSQKYIRRFSETSRISQRYTDYQRYRKSSS 60
           MSLDSE S SSS  RDWFFP  SF  S P+   +Y RRF                     
Sbjct: 1   MSLDSEVSVSSSSGRDWFFPSPSFFRSSPS---QYGRRFHTN------------------ 60

Query: 61  CISDSHSCSTTSNDVKFARTRRRFDFDRRSDLSLKRSELDSPSKQKLELPDVSSSAKKVS 120
             S++HS + +S      R RRR  F R            +P+    E P +S     VS
Sbjct: 61  --SNTHS-APSSTYPSGIRHRRRVKFSR------------TPTTSSNEKPQISI----VS 120

Query: 121 DSARLFRSLDSTLKVRWHFLAIAVSILVVIFATLVHENLSLQEQVNDLETRISNLYVKLR 180
           D        +     ++      V++ +V    L+  N  L+ QVN L+  I    ++L 
Sbjct: 121 DKPSAISKNNLNWLSQFGLQFALVTLTIVFLLLLLLRNTHLESQVNKLQGEI----LRLH 180

Query: 181 ACNLLD--SGSEDDVRSPDELADVASDKRLKTLALIASLTLLSAPVIILKYIDYISKSRS 240
           AC+ LD  + S        +    + +   + LAL  S  LL  P+II KYIDY+S+SR 
Sbjct: 181 ACHQLDTLNVSSSTAHKSQDTHPCSCENFKRNLALFLSFMLLLIPLIIFKYIDYVSRSRL 240

Query: 241 FDHNLEEVSLSKQLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDCLVDCL 300
            ++  E+VSL+KQ+AY+VDVF S++PYAKPL LL+ATLLLI LGGL LFGVT + L  CL
Sbjct: 241 SENISEQVSLNKQIAYRVDVFLSVYPYAKPLVLLVATLLLIFLGGLTLFGVTTEDLGHCL 300

Query: 301 WLSWTYVADSGNHANSEGIGPRLVSVSISFGGMLIFAMMLGLVSDSISEKFDSLRKGRSE 360
           WLSWTYVADSGNHA+SEGIGPRLV+VSISFGGMLIFAMMLGLVSD+ISEKFDSLRKG+SE
Sbjct: 301 WLSWTYVADSGNHASSEGIGPRLVAVSISFGGMLIFAMMLGLVSDAISEKFDSLRKGKSE 360

Query: 361 VVEQDHTLILGWSDKLGSLLNQIAIANESLGGGTVVVMAERDKEEMELEIAKMEFDFKGT 420
           VVEQ+HTLILGWSDKLGSLLNQ+AIANESLGGGT+ VMAERDKE+MEL+I KMEFDFKGT
Sbjct: 361 VVEQNHTLILGWSDKLGSLLNQLAIANESLGGGTIAVMAERDKEDMELDIGKMEFDFKGT 420

Query: 421 SVICRSGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVKEGLRGHI 480
           SVICRSGSPLILADLKKVSVSKAR IIV+AEDGNADQSDARALRTVLSLTGVKEGLRGHI
Sbjct: 421 SVICRSGSPLILADLKKVSVSKARTIIVLAEDGNADQSDARALRTVLSLTGVKEGLRGHI 480

Query: 481 VVELSDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYI 540
           VVE+SDLDNEVLVKLVGG+LVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYI
Sbjct: 481 VVEMSDLDNEVLVKLVGGDLVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYI 540

Query: 541 KRWPQLDGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLQEGDEVLVIAEDDDT 600
           KRWPQLDGM FEDVLISFP AIPCGIKVAS GGKI+LNP+DSYVLQEGDEVLVIAEDDDT
Sbjct: 541 KRWPQLDGMLFEDVLISFPAAIPCGIKVASYGGKIILNPDDSYVLQEGDEVLVIAEDDDT 600

Query: 601 YAPAALPTVREASFMHIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKDFIVPKSTEKI 660
           YAPA LP VR                                 RGSLPKDF+ PKS E+I
Sbjct: 601 YAPAPLPMVR---------------------------------RGSLPKDFVYPKSPERI 660

Query: 661 LLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLIDGGLDISRLENISLVDRE 720
           L CGWRRDMEDMI VLDA LAP SELWMFNDVPE EREKKLIDGGLDISRLENISLV+RE
Sbjct: 661 LFCGWRRDMEDMITVLDASLAPDSELWMFNDVPEKEREKKLIDGGLDISRLENISLVNRE 720

Query: 721 GNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLIRDIQGRPRSHVMTL 780
           GNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLIRDIQ R   +V   
Sbjct: 721 GNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLIRDIQARRLPYV--- 780

Query: 781 ALSTDEDGASMLREEDRAMTMTEEDVITMLVVTLMMSMDVVVIRAKRLPVRDAKIVGYRG 840
           A+++   G                                                   G
Sbjct: 781 AMASQTQG---------------------------------------------------G 840

Query: 841 SFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQ 900
           +FS+GSWIGEM+QASDK+VIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQ
Sbjct: 841 NFSKGSWIGEMKQASDKTVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQ 853

Query: 901 INDVLEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRREVVIGYRSANAERAVI 960
           INDVLEELFAEEGNE+HIRQAD+YLREGEE+SFYE++LRARQRRE++IGYR ANAERAVI
Sbjct: 901 INDVLEELFAEEGNEMHIRQADIYLREGEEMSFYEIMLRARQRREILIGYRLANAERAVI 853

Query: 961 NPPAKNERRKWSLGDVFVVIAEKE 981
           NPPAK  RRKWSL DVFVVI EKE
Sbjct: 961 NPPAKTGRRKWSLKDVFVVITEKE 853

BLAST of Spg025803 vs. ExPASy Swiss-Prot
Match: Q75LD5 (Probable ion channel CASTOR OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0843600 PE=2 SV=1)

HSP 1 Score: 1124.0 bits (2906), Expect = 0.0e+00
Identity = 637/988 (64.47%), Postives = 730/988 (73.89%), Query Frame = 0

Query: 1   MSLDSESSPS-SSRDWFFPP-QSFIHSYPAKSQKYIRRFSETSRISQRYTDYQRYRKSSS 60
           M LD +SSP+   RDWFFPP   F+ S  A++ +    F  TSR S  Y+   R R   +
Sbjct: 1   MPLDPDSSPAPPHRDWFFPPAPPFLPSSRARTPR--APFPSTSRSSNPYSFPDR-RPPPT 60

Query: 61  CISDSHSCSTTSNDVKFARTRRRF--DFDRRSD---LSLKRSELDSPSKQKLELPDVSSS 120
             S S S        K  +          RR D     ++R ++ + + +K        +
Sbjct: 61  PRSRSRSPLPPPEQQKQQQPPPTTPPPAPRRRDPRYAGVRRGDVRTLTAEKAAAAAAVPT 120

Query: 121 AKKVSDSARLFRSLDSTLKVRWHFLAIAVSILVVIFATLVHENLSLQEQVNDLETRISNL 180
           A +V  S    +S  S   +RW  + ++V+ +V+ F++LV  N SL +QV+ L+ +++  
Sbjct: 121 AAQVHGS----KSAASATTLRWSGM-VSVAAIVLCFSSLVRSNSSLHDQVHHLKAQLAEA 180

Query: 181 YVKLRACNLLDSGSEDDVRSPDELADVASDKRLKTLALIASLTLLSAPVIILKYIDYISK 240
             KL++C    S     + S       + ++ LK  +L+ SL+ L AP++ILKY+D   K
Sbjct: 181 TTKLQSCITESSMDMSSILSYQSNNSTSQNRGLKNFSLLLSLSTLYAPLLILKYMDLFLK 240

Query: 241 SRSFDHNLEEVSLSKQLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDCLV 300
            RS   + EEV ++K+LAY+VD+F S+ PYAKPL LL+ATLLLI LGGLAL+GV DD L+
Sbjct: 241 LRSSQDSEEEVPINKRLAYRVDIFLSLQPYAKPLVLLVATLLLIGLGGLALYGVNDDSLL 300

Query: 301 DCLWLSWTYVADSGNHANSEGIGPRLVSVSISFGGMLIFAMMLGLVSDSISEKFDSLRKG 360
           DCLWLSWT+VADSGNHAN+EG GP+LVSVSIS GGML+FAMMLGLV+DSISEKFDSLRKG
Sbjct: 301 DCLWLSWTFVADSGNHANAEGFGPKLVSVSISIGGMLVFAMMLGLVTDSISEKFDSLRKG 360

Query: 361 RSEVVEQDHTLILGWSDKLGSLLNQIAIANESLGGGTVVVMAERDKEEMELEIAKMEFDF 420
           RSEV+EQ HTL+LGWSDKLGSLLNQIAIANESLGGGT+VVMAE+DKEEME +IAKMEFD 
Sbjct: 361 RSEVIEQSHTLVLGWSDKLGSLLNQIAIANESLGGGTIVVMAEKDKEEMEADIAKMEFDL 420

Query: 421 KGTSVICRSGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVKEGLR 480
           KGT++ICRSGSPLILADLKKVSVSKARAI+V+AE+GNADQSDARALRTVLSLTGVKEGLR
Sbjct: 421 KGTAIICRSGSPLILADLKKVSVSKARAIVVLAEEGNADQSDARALRTVLSLTGVKEGLR 480

Query: 481 GHIVVELSDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCE 540
           GHIVVELSDLDNEVLVKLVGG+LVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCE
Sbjct: 481 GHIVVELSDLDNEVLVKLVGGDLVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCE 540

Query: 541 FYIKRWPQLDGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLQEGDEVLVIAED 600
           FYIKRWPQLDGMQFEDVLISFPDAIPCGIKVAS GGKI+LNP+D YVLQEGDEVLVIAED
Sbjct: 541 FYIKRWPQLDGMQFEDVLISFPDAIPCGIKVASYGGKIILNPDDFYVLQEGDEVLVIAED 600

Query: 601 DDTYAPAALPTVREASFMHIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKDFIVPKST 660
           DDTYAPA LP                                  V RG LPKDF+VPKS 
Sbjct: 601 DDTYAPAPLPK---------------------------------VMRGYLPKDFVVPKSP 660

Query: 661 EKILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLIDGGLDISRLENISLV 720
           E+IL CGWRRDMEDMIMVLDAFLAPGSELWMFNDVPE +RE+KLIDGGLD SRLENI+LV
Sbjct: 661 ERILFCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPEMDRERKLIDGGLDFSRLENITLV 720

Query: 721 DREGNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLIRDIQGRPRSHV 780
            REGNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLIRDIQ       
Sbjct: 721 HREGNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLIRDIQ------- 780

Query: 781 MTLALSTDEDGASMLREEDRAMTMTEEDVITMLVVTLMMSMDVVVIRAKRLPVRDAKIVG 840
                                                          AKRLP R+A +  
Sbjct: 781 -----------------------------------------------AKRLPFREAMVSH 840

Query: 841 Y-RGSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVA 900
             RGSF +GSWIGEMQQASDKSVIISEILDPRTKNLLS+SKISDYVLSNELVSMALAMVA
Sbjct: 841 VTRGSFCEGSWIGEMQQASDKSVIISEILDPRTKNLLSVSKISDYVLSNELVSMALAMVA 893

Query: 901 EDRQINDVLEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRREVVIGYRSANAE 960
           EDRQINDVLEELFAE+GNE+ IR ADLYLRE EEL+F+EV+LR RQR+E+VIGYR  +AE
Sbjct: 901 EDRQINDVLEELFAEQGNEMQIRPADLYLREDEELNFFEVMLRGRQRKEIVIGYRLVDAE 893

Query: 961 RAVINPPAKNERRKWSLGDVFVVIAEKE 981
           RA+INPP K  RR+WS  DVFVVI EKE
Sbjct: 961 RAIINPPDKVSRRRWSAKDVFVVITEKE 893

BLAST of Spg025803 vs. ExPASy Swiss-Prot
Match: Q6RHR6 (Ion channel DMI1 OS=Medicago truncatula OX=3880 GN=DMI1 PE=1 SV=1)

HSP 1 Score: 951.0 bits (2457), Expect = 1.0e-275
Identity = 523/842 (62.11%), Postives = 611/842 (72.57%), Query Frame = 0

Query: 144 ILVVIFATLVHENLSLQEQVNDLETRISNLYVKLRACNLLDSGSEDDVRSPDELADVASD 203
           +LV+    LV  +  LQ ++  L+       +KL+ C  +D  S +   S  E  D   +
Sbjct: 134 LLVICCIILVPYSAYLQYKLAKLKD------MKLQLCGQIDFCSRNGKTSIQEEVDDDDN 193

Query: 204 KRLKTLALIASLTLLSAPVIILKYIDYISKSRSF----DHNLEEVSLSKQLAYKVDVFFS 263
              +T+AL   L  L  P ++ KY+DY+ +  +F    + N E+V L K++AY VDVFFS
Sbjct: 194 ADSRTIALYIVLFTLILPFVLYKYLDYLPQIINFLRRTESNKEDVPLKKRVAYMVDVFFS 253

Query: 264 IHPYAKPLALLIATLLLIMLGGLALFGVTDDCLVDCLWLSWTYVADSGNHANSEGIGPRL 323
           I+PYAK LALL ATL LI  GGLAL+ VT   + + LW SWTYVAD+GNHA +EG G R+
Sbjct: 254 IYPYAKLLALLCATLFLIAFGGLALYAVTGGSMAEALWHSWTYVADAGNHAETEGTGQRI 313

Query: 324 VSVSISFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQDHTLILGWSDKLGSLLNQI 383
           VSVSIS GGMLIFAMMLGLVSD+ISEK DSLRKG+SEV+E++H LILGWSDKLGSLL Q+
Sbjct: 314 VSVSISAGGMLIFAMMLGLVSDAISEKVDSLRKGKSEVIERNHVLILGWSDKLGSLLKQL 373

Query: 384 AIANESLGGGTVVVMAERDKEEMELEIAKMEFDFKGTSVICRSGSPLILADLKKVSVSKA 443
           AIAN+S+GGG +VV+AE++KEEME++IAK+EFDF GTSVICRSGSPLILADLKKVSVSKA
Sbjct: 374 AIANKSVGGGVIVVLAEKEKEEMEMDIAKLEFDFMGTSVICRSGSPLILADLKKVSVSKA 433

Query: 444 RAIIVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVELSDLDNEVLVKLVGGELVET 503
           RAIIV+A D NADQSDARALR VLSL GVKEGLRGH+VVE+SDLDNE LVKLVGGEL+ET
Sbjct: 434 RAIIVLAADENADQSDARALRVVLSLAGVKEGLRGHVVVEMSDLDNEPLVKLVGGELIET 493

Query: 504 VVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWPQLDGMQFEDVLISFPDAIP 563
           VVAHDVIGRLMIQCA QPGLAQIWEDILGFEN EFYIKRWP+LD + F+D+LISFPDAIP
Sbjct: 494 VVAHDVIGRLMIQCALQPGLAQIWEDILGFENAEFYIKRWPELDDLLFKDILISFPDAIP 553

Query: 564 CGIKVASRGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPAALPTVREASFMHIARPTRK 623
           CG+KVA+ GGKIV+NP+D+YVL++GDEVLVIAEDDDTYAP  LP VR+  F  I  P + 
Sbjct: 554 CGVKVAADGGKIVINPDDNYVLRDGDEVLVIAEDDDTYAPGPLPEVRKGYFPRIRDPPKY 613

Query: 624 PQKILLCGWRRDIDDMIVVWRGSLPKDFIVPKSTEKILLCGWRRDMEDMIMVLDAFLAPG 683
           P+KIL CGWRRDID                                 DMIMVL+AFLAPG
Sbjct: 614 PEKILFCGWRRDID---------------------------------DMIMVLEAFLAPG 673

Query: 684 SELWMFNDVPENEREKKLIDGGLDISRLENISLVDREGNAVIRRHLESLPLESFDSILIL 743
           SELWMFN+VPE ERE+KL  G LD+  LENI LV REGNAVIRRHLESLPLE+FDSILIL
Sbjct: 674 SELWMFNEVPEKERERKLAAGELDVFGLENIKLVHREGNAVIRRHLESLPLETFDSILIL 733

Query: 744 ADESVEDSAIQADSRSLATLLLIRDIQGRPRSHVMTLALSTDEDGASMLREEDRAMTMTE 803
           ADESVEDS   +DSRSLATLLLIRDIQ R                               
Sbjct: 734 ADESVEDSVAHSDSRSLATLLLIRDIQSR------------------------------- 793

Query: 804 EDVITMLVVTLMMSMDVVVIRAKRLPVRDAKIVGYR-GSFSQGSWIGEMQQASDKSVIIS 863
                                  RLP RD K    R   FS  SWI EMQQASDKS+IIS
Sbjct: 794 -----------------------RLPYRDTKSTSLRLSGFSHNSWIREMQQASDKSIIIS 853

Query: 864 EILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFAEEGNELHIRQAD 923
           EILD RT+NL+S+S+ISDYVLSNELVSMALAMVAED+QIN VLEELFAEEGNE+ I+ A+
Sbjct: 854 EILDSRTRNLVSVSRISDYVLSNELVSMALAMVAEDKQINRVLEELFAEEGNEMCIKPAE 882

Query: 924 LYLREGEELSFYEVLLRARQRREVVIGYRSANAERAVINPPAKNERRKWSLGDVFVVIAE 981
            YL + EEL FY++++R R R+E+VIGYR AN ERA+INP  K+  RKWSL DVFVV+A 
Sbjct: 914 FYLFDQEELCFYDIMIRGRTRKEIVIGYRLANQERAIINPSEKSVPRKWSLDDVFVVLAS 882

BLAST of Spg025803 vs. ExPASy Swiss-Prot
Match: Q4VY51 (Probable ion channel SYM8 OS=Pisum sativum OX=3888 GN=SYM8 PE=1 SV=3)

HSP 1 Score: 945.7 bits (2443), Expect = 4.3e-274
Identity = 510/779 (65.47%), Postives = 587/779 (75.35%), Query Frame = 0

Query: 207 KTLALIASLTLLSAPVIILKYIDY----ISKSRSFDHNLEEVSLSKQLAYKVDVFFSIHP 266
           +T++L   L  L  P I+ KYIDY    I+ SR  + N E+V L K++AY VDVFFSI+P
Sbjct: 203 RTISLYIVLFTLVLPFILYKYIDYLPQMINFSRRTNSNKEDVPLKKRVAYMVDVFFSIYP 262

Query: 267 YAKPLALLIATLLLIMLGGLALFGVTDDCLVDCLWLSWTYVADSGNHANSEGIGPRLVSV 326
           YAK LALL ATL LI  GGLAL+ VT   + + LW SWTYVAD+GNHA +EG+G R+VSV
Sbjct: 263 YAKLLALLFATLFLIAFGGLALYAVTGGSMAEALWHSWTYVADAGNHAETEGMGQRIVSV 322

Query: 327 SISFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQDHTLILGWSDKLGSLLNQIAIA 386
           SIS GGMLIFAMMLGLVSD+ISEK DSLRKG+SEV+E++H LILGWSDKLGSLL Q+AIA
Sbjct: 323 SISAGGMLIFAMMLGLVSDAISEKVDSLRKGKSEVIERNHVLILGWSDKLGSLLKQLAIA 382

Query: 387 NESLGGGTVVVMAERDKEEMELEIAKMEFDFKGTSVICRSGSPLILADLKKVSVSKARAI 446
           N+S+GGG +VV+AE++KEEME++IAK+EFDF GTSVICRSGSPLILADLKKVSVSKARAI
Sbjct: 383 NKSVGGGVIVVLAEKEKEEMEMDIAKLEFDFMGTSVICRSGSPLILADLKKVSVSKARAI 442

Query: 447 IVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVELSDLDNEVLVKLVGGELVETVVA 506
           IV+A D NADQSDARALR VLSLTGVKE LRGH+VVE+SDLDNE LVKLVGGEL+ETVVA
Sbjct: 443 IVLASDENADQSDARALRVVLSLTGVKEALRGHVVVEMSDLDNEPLVKLVGGELIETVVA 502

Query: 507 HDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWPQLDGMQFEDVLISFPDAIPCGI 566
           HDVIGRLMIQCA QPGLAQIWEDILGFEN EFYIKRWP+LDG+ F+D+LISFPDAIPCG+
Sbjct: 503 HDVIGRLMIQCALQPGLAQIWEDILGFENAEFYIKRWPELDGLLFKDILISFPDAIPCGV 562

Query: 567 KVASRGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPAALPTVREASFMHIARPTRKPQK 626
           KV++ GGKIV+NP+D+YVL++GDEVLVIAEDDDTYAP  LP VR+  F  I  P + P+K
Sbjct: 563 KVSADGGKIVINPDDNYVLRDGDEVLVIAEDDDTYAPGPLPEVRKGYFPRIRDPPKYPEK 622

Query: 627 ILLCGWRRDIDDMIVVWRGSLPKDFIVPKSTEKILLCGWRRDMEDMIMVLDAFLAPGSEL 686
           IL CGWRRDID                                 DMIMVL+AFLAPGSEL
Sbjct: 623 ILFCGWRRDID---------------------------------DMIMVLEAFLAPGSEL 682

Query: 687 WMFNDVPENEREKKLIDGGLDISRLENISLVDREGNAVIRRHLESLPLESFDSILILADE 746
           WMFN+VPE +RE+KL  G LD+  LENI LV REGNAVIRRHLESLPLE+FDSILILADE
Sbjct: 683 WMFNEVPEKQRERKLAAGELDVFGLENIKLVHREGNAVIRRHLESLPLETFDSILILADE 742

Query: 747 SVEDSAIQADSRSLATLLLIRDIQGRPRSHVMTLALSTDEDGASMLREEDRAMTMTEEDV 806
           SVEDS   +DSRSLATLLLIRDIQ R                                  
Sbjct: 743 SVEDSVAHSDSRSLATLLLIRDIQSR---------------------------------- 802

Query: 807 ITMLVVTLMMSMDVVVIRAKRLPVRDAKIVGYR-GSFSQGSWIGEMQQASDKSVIISEIL 866
                               RLP RD K    R   FS  SWI EMQQASDKS+IISEIL
Sbjct: 803 --------------------RLPYRDTKSTSLRLSGFSHNSWIREMQQASDKSIIISEIL 862

Query: 867 DPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFAEEGNELHIRQADLYL 926
           D RT+NL+S+S+ISDYVLSNELVSMALAMVAED+QIN VLEELFAEEGNE+ I+ A+ YL
Sbjct: 863 DSRTRNLVSVSRISDYVLSNELVSMALAMVAEDKQINRVLEELFAEEGNEMCIKPAEFYL 894

Query: 927 REGEELSFYEVLLRARQRREVVIGYRSANAERAVINPPAKNERRKWSLGDVFVVIAEKE 981
            + EEL FY++++R R R+E+VIGYR A+ ERA+INP  K+  RKWSL DVFVVIA  E
Sbjct: 923 FDQEELCFYDIMIRGRTRKEIVIGYRLASQERALINPSEKSMTRKWSLDDVFVVIASGE 894

BLAST of Spg025803 vs. ExPASy Swiss-Prot
Match: Q5N941 (Probable ion channel POLLUX OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0870100 PE=2 SV=1)

HSP 1 Score: 940.3 bits (2429), Expect = 1.8e-272
Identity = 504/774 (65.12%), Postives = 586/774 (75.71%), Query Frame = 0

Query: 209 LALIASLTLLSAPVIILKYIDYISKSRSFDHNL----EEVSLSKQLAYKVDVFFSIHPYA 268
           LA  + + + + PV ++KYID + +  +    L    EEV L K++AYKVDVFFS HPYA
Sbjct: 255 LASCSRMLVFAIPVFLVKYIDQLRRRNTDSIRLRSTEEEVPLKKRIAYKVDVFFSGHPYA 314

Query: 269 KPLALLIATLLLIMLGGLALFGVTDDCLVDCLWLSWTYVADSGNHANSEGIGPRLVSVSI 328
           K LALL+AT++LI  GG+AL+ V+    ++ LWLSWT+VADSGNHA+  G+GPR+VSVSI
Sbjct: 315 KLLALLLATIILIASGGIALYVVSGSGFLEALWLSWTFVADSGNHADQVGLGPRIVSVSI 374

Query: 329 SFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQDHTLILGWSDKLGSLLNQIAIANE 388
           S GGML+FA MLGLVSD+ISEK DS RKG+SEV+E +H LILGWSDKLGSLL Q+AIAN+
Sbjct: 375 SSGGMLVFATMLGLVSDAISEKVDSWRKGKSEVIEVNHILILGWSDKLGSLLKQLAIANK 434

Query: 389 SLGGGTVVVMAERDKEEMELEIAKMEFDFKGTSVICRSGSPLILADLKKVSVSKARAIIV 448
           S+GGG VVV+AERDKEEME++I K+EFDF GTSVICRSGSPLILADLKKVSVSKARAIIV
Sbjct: 435 SIGGGVVVVLAERDKEEMEMDIGKLEFDFMGTSVICRSGSPLILADLKKVSVSKARAIIV 494

Query: 449 IAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVELSDLDNEVLVKLVGGELVETVVAHD 508
           +A D NADQSDARALR VLSLTGVKEGLRGH+VVE+SDLDNE LVKLVGGEL+ETVVAHD
Sbjct: 495 LASDENADQSDARALRVVLSLTGVKEGLRGHVVVEMSDLDNEPLVKLVGGELIETVVAHD 554

Query: 509 VIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWPQLDGMQFEDVLISFPDAIPCGIKV 568
           VIGRLMIQCA QPGLAQIWEDILGFEN EFYIKRWP+LDGM+F DVLISFPDA+PCG+K+
Sbjct: 555 VIGRLMIQCALQPGLAQIWEDILGFENAEFYIKRWPELDGMRFGDVLISFPDAVPCGVKI 614

Query: 569 ASRGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPAALPTVREASFMHIARPTRKPQKIL 628
           AS+ GKI++NP++ YVLQEGDEVLVIAEDDDTY PA+LP VR                  
Sbjct: 615 ASKAGKILMNPDNDYVLQEGDEVLVIAEDDDTYVPASLPQVR------------------ 674

Query: 629 LCGWRRDIDDMIVVWRGSLPKDFIVPKSTEKILLCGWRRDMEDMIMVLDAFLAPGSELWM 688
                          +G LP     PK  EKIL CGWRRD+ DMIMVL+AFLAPGSELWM
Sbjct: 675 ---------------KGFLPNIPTPPKYPEKILFCGWRRDIHDMIMVLEAFLAPGSELWM 734

Query: 689 FNDVPENEREKKLIDGGLDISRLENISLVDREGNAVIRRHLESLPLESFDSILILADESV 748
           FN+VPE ERE+KL DGG+DI  L NI LV +EGNAVIRRHLESLPLE+FDSILILADESV
Sbjct: 735 FNEVPEKERERKLTDGGMDIYGLTNIKLVHKEGNAVIRRHLESLPLETFDSILILADESV 794

Query: 749 EDSAIQADSRSLATLLLIRDIQGRPRSHVMTLALSTDEDGASMLREEDRAMTMTEEDVIT 808
           EDS + +DSRSLATLLLIRDIQ                                      
Sbjct: 795 EDSIVHSDSRSLATLLLIRDIQ-------------------------------------- 854

Query: 809 MLVVTLMMSMDVVVIRAKRLPVRDAKIVGYRGSFSQGSWIGEMQQASDKSVIISEILDPR 868
                           +KRLP ++ K       F   SWI EMQ ASDKS+IISEILD R
Sbjct: 855 ----------------SKRLPSKELKSPLRYNGFCHSSWIREMQHASDKSIIISEILDSR 914

Query: 869 TKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFAEEGNELHIRQADLYLREG 928
           T+NL+S+SKISDYVLSNELVSMALAMVAED+QIN VLEELFAEEGNE+ IR A+ YL E 
Sbjct: 915 TRNLVSVSKISDYVLSNELVSMALAMVAEDKQINRVLEELFAEEGNEMCIRSAEFYLYEQ 941

Query: 929 EELSFYEVLLRARQRREVVIGYRSANAERAVINPPAKNERRKWSLGDVFVVIAE 979
           EELSF+++++RAR+R EVVIGYR AN ++A+INP  K+E RKWSL DVFVVI++
Sbjct: 975 EELSFFDIMVRARERDEVVIGYRLANDDQAIINPEQKSEIRKWSLDDVFVVISK 941

BLAST of Spg025803 vs. ExPASy TrEMBL
Match: A0A5A7SU61 (Ion channel CASTOR-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G001710 PE=3 SV=1)

HSP 1 Score: 1637.1 bits (4238), Expect = 0.0e+00
Identity = 861/980 (87.86%), Postives = 896/980 (91.43%), Query Frame = 0

Query: 1   MSLDSESSPSSSRDWFFPPQSFIHSYPAKSQKYIRRFSETSRISQRYTDYQRYRKSSSCI 60
           MSLDSESSPSSSRDWFFPPQSF+HS+PAKS  YIRRFS+TSR+S+RYTDY RYRK+SSCI
Sbjct: 1   MSLDSESSPSSSRDWFFPPQSFLHSHPAKSPNYIRRFSDTSRLSRRYTDYHRYRKTSSCI 60

Query: 61  SDSHSCSTTSNDVKFARTRRRFDFDRRSDLSLKRSELDSPSKQKLELPDVSSSAKKVSDS 120
           SDSHSCST +NDVKFAR+RRRF FDRRSDLSLKRSE++  SK KLELPDVSSSAKKVSD+
Sbjct: 61  SDSHSCSTITNDVKFARSRRRFYFDRRSDLSLKRSEVEFSSKPKLELPDVSSSAKKVSDT 120

Query: 121 ARLFRSLDSTLKVRWHFLAIAVSILVVIFATLVHENLSLQEQVNDLETRISNLYVKLRAC 180
           +RL +S DS+LKVRW FLAIA SI VV FAT+VHENLSLQEQVN+LETRISNL +KLRAC
Sbjct: 121 SRLSKSFDSSLKVRWLFLAIAASIFVVSFATIVHENLSLQEQVNNLETRISNLNIKLRAC 180

Query: 181 NLLDSGSEDDVRSPDELADVASDKRLKTLALIASLTLLSAPVIILKYIDYISKSRSFDHN 240
           NL D G+EDDVRSPDELADV +DKRLKTLALIASLTLL AP+IILKYIDYISKSRS DHN
Sbjct: 181 NLFDHGNEDDVRSPDELADVVTDKRLKTLALIASLTLLFAPIIILKYIDYISKSRSLDHN 240

Query: 241 LEEVSLSKQLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDCLVDCLWLSW 300
           LEE+SL+K+LAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDD LVDCLWLSW
Sbjct: 241 LEEISLNKRLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDNLVDCLWLSW 300

Query: 301 TYVADSGNHANSEGIGPRLVSVSISFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ 360
           TYVADSGNHANSEG GPRLVSVS+SFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ
Sbjct: 301 TYVADSGNHANSEGFGPRLVSVSVSFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ 360

Query: 361 DHTLILGWSDKLGSLLNQIAIANESLGGGTVVVMAERDKEEMELEIAKMEFDFKGTSVIC 420
           +HTLILGWSDKLGSLLNQI+IANESLGGG VVVMAERDKEEMEL+IAKMEFDFKGTSVIC
Sbjct: 361 NHTLILGWSDKLGSLLNQISIANESLGGGIVVVMAERDKEEMELDIAKMEFDFKGTSVIC 420

Query: 421 RSGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL 480
           R+GSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL
Sbjct: 421 RTGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL 480

Query: 481 SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540
           SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP
Sbjct: 481 SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540

Query: 541 QLDGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA 600
           QL GMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVL+EGDEVLVIAEDDDTYAPA
Sbjct: 541 QLTGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLEEGDEVLVIAEDDDTYAPA 600

Query: 601 ALPTVREASFMHIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKDFIVPKSTEKILLCG 660
            LPTVREASF+HIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKDFIVPKS E+ILLCG
Sbjct: 601 PLPTVREASFIHIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKDFIVPKSAERILLCG 660

Query: 661 WRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLIDGGLDISRLENISLVDREGNAV 720
           WRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKL+DGGLDISRLENISLVDREGNAV
Sbjct: 661 WRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLVDGGLDISRLENISLVDREGNAV 720

Query: 721 IRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLIRDIQGRPRSHVMTLALST 780
           IRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLIRDIQ              
Sbjct: 721 IRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLIRDIQ-------------- 780

Query: 781 DEDGASMLREEDRAMTMTEEDVITMLVVTLMMSMDVVVIRAKRLPVRDAKIVGYRGSFSQ 840
                                                   AKR+PVRDAK   ++GSFSQ
Sbjct: 781 ----------------------------------------AKRMPVRDAKATAHKGSFSQ 840

Query: 841 GSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDV 900
           GSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDV
Sbjct: 841 GSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDV 900

Query: 901 LEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRREVVIGYRSANAERAVINPPA 960
           LEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRREVVIGYRSANAERAVINPPA
Sbjct: 901 LEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRREVVIGYRSANAERAVINPPA 926

Query: 961 KNERRKWSLGDVFVVIAEKE 981
           KNERRKWSL DVFVVIAEKE
Sbjct: 961 KNERRKWSLRDVFVVIAEKE 926

BLAST of Spg025803 vs. ExPASy TrEMBL
Match: A0A5D3CV00 (Ion channel CASTOR-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G002430 PE=3 SV=1)

HSP 1 Score: 1628.2 bits (4215), Expect = 0.0e+00
Identity = 861/992 (86.79%), Postives = 896/992 (90.32%), Query Frame = 0

Query: 1   MSLDSESSPSSSRDWFFPPQSFIHSYPAKSQKYIRRFSETSRISQRYTDYQRYRKSSSCI 60
           MSLDSESSPSSSRDWFFPPQSF+HS+PAKS  YIRRFS+TSR+S+RYTDY RYRK+SSCI
Sbjct: 1   MSLDSESSPSSSRDWFFPPQSFLHSHPAKSPNYIRRFSDTSRLSRRYTDYHRYRKTSSCI 60

Query: 61  SDSHSCSTTSNDVKFARTRRRFDFDRRSDLSLKRSELDSPSKQKLELPDVSSSAKKVSDS 120
           SDSHSCST +NDVKFAR+RRRF FDRRSDLSLKRSE++  SK KLELPDVSSSAKKVSD+
Sbjct: 61  SDSHSCSTITNDVKFARSRRRFYFDRRSDLSLKRSEVEFSSKPKLELPDVSSSAKKVSDT 120

Query: 121 ARLFRSLDSTLKVRWHFLAIAVSILVVIFATLVHENLSLQEQVNDLETRISNLYVKLRAC 180
           +RL +S DS+LKVRW FLAIA SI VV FAT+VHENLSLQEQVN+LETRISNL +KLRAC
Sbjct: 121 SRLSKSFDSSLKVRWLFLAIAASIFVVSFATIVHENLSLQEQVNNLETRISNLNIKLRAC 180

Query: 181 NLLDSGSEDDVRSPDELADVASDKRLKTLALIASLTLLSAPVIILKYIDYISKSRSFDHN 240
           NL D G+EDDVRSPDELADV +DKRLKTLALIASLTLL AP+IILKYIDYISKSRS DHN
Sbjct: 181 NLFDHGNEDDVRSPDELADVVTDKRLKTLALIASLTLLFAPIIILKYIDYISKSRSLDHN 240

Query: 241 LEEVSLSKQLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDCLVDCLWLSW 300
           LEE+SL+K+LAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDD LVDCLWLSW
Sbjct: 241 LEEISLNKRLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDNLVDCLWLSW 300

Query: 301 TYVADSGNHANSEGIGPRLVSVSISFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ 360
           TYVADSGNHANSEG GPRLVSVS+SFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ
Sbjct: 301 TYVADSGNHANSEGFGPRLVSVSVSFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ 360

Query: 361 DHTLILGWSDKLGSLLNQIAIANESLGGGTVVVMAERDKEEMELEIAKMEFDFKGTSVIC 420
           +HTLILGWSDKLGSLLNQI+IANESLGGG VVVMAERDKEEMEL+IAKMEFDFKGTSVIC
Sbjct: 361 NHTLILGWSDKLGSLLNQISIANESLGGGIVVVMAERDKEEMELDIAKMEFDFKGTSVIC 420

Query: 421 RSGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL 480
           R+GSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL
Sbjct: 421 RTGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL 480

Query: 481 SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540
           SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP
Sbjct: 481 SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540

Query: 541 QLDGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA 600
           QL GMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVL+EGDEVLVIAEDDDTYAPA
Sbjct: 541 QLTGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLEEGDEVLVIAEDDDTYAPA 600

Query: 601 ALPTVREASFMHIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKDFIVPKSTEKILLCG 660
            LPTVREASF+HIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKDFIVPKS E+ILLCG
Sbjct: 601 PLPTVREASFIHIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKDFIVPKSAERILLCG 660

Query: 661 WRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLIDGGLDISRLENISLVDREGNAV 720
           WRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKL+DGGLDISRLENISLVDREGNAV
Sbjct: 661 WRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLVDGGLDISRLENISLVDREGNAV 720

Query: 721 IRRHLESLPLESFDS------------ILILADESVEDSAIQADSRSLATLLLIRDIQGR 780
           IRRHLESLPLESFDS            ILILADESVEDSAIQADSRSLATLLLIRDIQ  
Sbjct: 721 IRRHLESLPLESFDSVSSVISFHPGSEILILADESVEDSAIQADSRSLATLLLIRDIQ-- 780

Query: 781 PRSHVMTLALSTDEDGASMLREEDRAMTMTEEDVITMLVVTLMMSMDVVVIRAKRLPVRD 840
                                                               AKR+PVRD
Sbjct: 781 ----------------------------------------------------AKRMPVRD 840

Query: 841 AKIVGYRGSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMAL 900
           AK   ++GSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMAL
Sbjct: 841 AKATAHKGSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMAL 900

Query: 901 AMVAEDRQINDVLEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRREVVIGYRS 960
           AMVAEDRQINDVLEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRREVVIGYRS
Sbjct: 901 AMVAEDRQINDVLEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRREVVIGYRS 938

Query: 961 ANAERAVINPPAKNERRKWSLGDVFVVIAEKE 981
           ANAERAVINPPAKNERRKWSL DVFVVIAEKE
Sbjct: 961 ANAERAVINPPAKNERRKWSLRDVFVVIAEKE 938

BLAST of Spg025803 vs. ExPASy TrEMBL
Match: A0A6J1GWH3 (ion channel CASTOR-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458157 PE=3 SV=1)

HSP 1 Score: 1610.5 bits (4169), Expect = 0.0e+00
Identity = 857/998 (85.87%), Postives = 890/998 (89.18%), Query Frame = 0

Query: 1   MSLDSESSPSSSRDWFFPPQSFIHSYPAKSQKYIRRFSETSRISQRYTDYQRYRKSSSCI 60
           MSLDSESSPSS+RDWFFPPQSF+HSYPAKS  YIRRFSETSRIS+RY D+QRYRKSSSCI
Sbjct: 1   MSLDSESSPSSTRDWFFPPQSFLHSYPAKSPTYIRRFSETSRISRRYADHQRYRKSSSCI 60

Query: 61  SDSHSCSTTSNDVKFARTRRRFDFDRRSDLSLKRSELDSPSKQKLELPDVSSSAKKVSDS 120
           SDSHSCS+TSND KFARTRRR DFDRRSDLSLKRSE +  SK+KLE  DVSSSAKKVSD+
Sbjct: 61  SDSHSCSSTSNDAKFARTRRRIDFDRRSDLSLKRSESEFSSKRKLEQSDVSSSAKKVSDT 120

Query: 121 ARLFRSLDSTLKVRWHFLAIAVSILVVIFATLVHENLSLQEQVNDLETRISNLYVKLRAC 180
           +RLFRS DSTLKVRW FLAIA SI +VIFATL+HEN SLQEQVN LE RISNL +KLRAC
Sbjct: 121 SRLFRSFDSTLKVRWRFLAIAASIFIVIFATLLHENSSLQEQVNGLEARISNLNIKLRAC 180

Query: 181 NLLDSGSEDDVRSPDELADVASDKRLKTLALIASLTLLSAPVIILKYIDYISKSRSFDHN 240
           NL DSGSEDDVRSPDELAD   DKRLKTLALIASLTLL AP++ILKYID  SKSRS +HN
Sbjct: 181 NLFDSGSEDDVRSPDELADF-MDKRLKTLALIASLTLLLAPIVILKYID--SKSRSLEHN 240

Query: 241 LEEVSLSKQLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDCLVDCLWLSW 300
           LEEVSL+KQL YKVDVFFS+HPYAKPLALLIATLLLIMLGGLALFGVTDD LVDCLWLSW
Sbjct: 241 LEEVSLNKQLLYKVDVFFSVHPYAKPLALLIATLLLIMLGGLALFGVTDDSLVDCLWLSW 300

Query: 301 TYVADSGNHANSEGIGPRLVSVSISFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ 360
           TYVADSGNHANSEGIGPRLVSVS+SFGGMLIFAMMLGLVSDSISE+FDSLRKGRSEVVEQ
Sbjct: 301 TYVADSGNHANSEGIGPRLVSVSVSFGGMLIFAMMLGLVSDSISERFDSLRKGRSEVVEQ 360

Query: 361 DHTLILGWSDKLGSLLNQIAIANESLGGGTVVVMAERDKEEMELEIAKMEFDFKGTSVIC 420
           +HTLILGWSDKLGSLLNQI+IANESLGGGTVVVMAERDKEEMEL+IAKMEFDFKGTSVIC
Sbjct: 361 NHTLILGWSDKLGSLLNQISIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVIC 420

Query: 421 RSGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL 480
           R+GSPLILADLKKVSVSKARAI+VIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL
Sbjct: 421 RTGSPLILADLKKVSVSKARAIVVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL 480

Query: 481 SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540
           SDLDNE+LVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP
Sbjct: 481 SDLDNELLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540

Query: 541 QLDGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA 600
           QL+GMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA
Sbjct: 541 QLNGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA 600

Query: 601 ALPTVREASFMHIARPTRKPQKILLCGWRRDIDDMIV------------------VWRGS 660
           ALPTV E SF+HIARPTRKPQKILLCGWRRDIDDMIV                  VWRGS
Sbjct: 601 ALPTVGETSFIHIARPTRKPQKILLCGWRRDIDDMIVVSAFIKALILSFWHWMLQVWRGS 660

Query: 661 LPKDFIVPKSTEKILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLIDGGL 720
           LPKDFIVPKS E+ILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKL+DGGL
Sbjct: 661 LPKDFIVPKSAERILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLVDGGL 720

Query: 721 DISRLENISLVDREGNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLI 780
           DISRLENISLVDREGNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLI
Sbjct: 721 DISRLENISLVDREGNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLI 780

Query: 781 RDIQGRPRSHVMTLALSTDEDGASMLREEDRAMTMTEEDVITMLVVTLMMSMDVVVIRAK 840
           RDIQ                                                      AK
Sbjct: 781 RDIQ------------------------------------------------------AK 840

Query: 841 RLPVRDAKIVGYRGSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNE 900
           R+PVR AK V YRGS SQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNE
Sbjct: 841 RMPVRGAKTVAYRGSVSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNE 900

Query: 901 LVSMALAMVAEDRQINDVLEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRREV 960
           LVSMALAMVAEDRQINDVLEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRRE+
Sbjct: 901 LVSMALAMVAEDRQINDVLEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRREI 941

Query: 961 VIGYRSANAERAVINPPAKNERRKWSLGDVFVVIAEKE 981
           VIGYRSA+AERAVINPPAK+ERRKW LGDVFVVIAEKE
Sbjct: 961 VIGYRSAHAERAVINPPAKHERRKWHLGDVFVVIAEKE 941

BLAST of Spg025803 vs. ExPASy TrEMBL
Match: A0A6J1K6V9 (ion channel CASTOR-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492175 PE=3 SV=1)

HSP 1 Score: 1609.7 bits (4167), Expect = 0.0e+00
Identity = 858/998 (85.97%), Postives = 889/998 (89.08%), Query Frame = 0

Query: 1   MSLDSESSPSSSRDWFFPPQSFIHSYPAKSQKYIRRFSETSRISQRYTDYQRYRKSSSCI 60
           MSLDSESSPSSSRDWFFPPQSF+ SYPAKSQ YIRRFSETSRIS+RY D+QRYRKSSSCI
Sbjct: 1   MSLDSESSPSSSRDWFFPPQSFVQSYPAKSQTYIRRFSETSRISRRYADHQRYRKSSSCI 60

Query: 61  SDSHSCSTTSNDVKFARTRRRFDFDRRSDLSLKRSELDSPSKQKLELPDVSSSAKKVSDS 120
           SDSHSCS+TSND KFARTRRR DFDRRSDLSLKRSEL+  SK+KLE  DVSSSAKKVSD+
Sbjct: 61  SDSHSCSSTSNDAKFARTRRRIDFDRRSDLSLKRSELEFSSKRKLEQSDVSSSAKKVSDT 120

Query: 121 ARLFRSLDSTLKVRWHFLAIAVSILVVIFATLVHENLSLQEQVNDLETRISNLYVKLRAC 180
           +RLFRS DSTLKVRW FLAIA SI +VIFATL+HEN SLQEQVN LE RISNL +KLRAC
Sbjct: 121 SRLFRSFDSTLKVRWRFLAIAASIFIVIFATLLHENSSLQEQVNGLEARISNLNIKLRAC 180

Query: 181 NLLDSGSEDDVRSPDELADVASDKRLKTLALIASLTLLSAPVIILKYIDYISKSRSFDHN 240
           NL DSGSEDDVRSPDELAD   DKRLKTLALI SLTLL AP+IILKYID  SKSRS +HN
Sbjct: 181 NLFDSGSEDDVRSPDELADF-MDKRLKTLALITSLTLLLAPIIILKYID--SKSRSLEHN 240

Query: 241 LEEVSLSKQLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDCLVDCLWLSW 300
           LEE+SL+KQL YKVDVFFS+HPYAKPLALLIATL LIMLGGLALFGVTDD LVDCLWLSW
Sbjct: 241 LEEISLNKQLLYKVDVFFSVHPYAKPLALLIATLQLIMLGGLALFGVTDDSLVDCLWLSW 300

Query: 301 TYVADSGNHANSEGIGPRLVSVSISFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ 360
           TYVADSGNHANSEGIGPRLVSVS+SFGGMLIFAMMLGLVSDSISE+FDSLRKGRSEVVEQ
Sbjct: 301 TYVADSGNHANSEGIGPRLVSVSVSFGGMLIFAMMLGLVSDSISERFDSLRKGRSEVVEQ 360

Query: 361 DHTLILGWSDKLGSLLNQIAIANESLGGGTVVVMAERDKEEMELEIAKMEFDFKGTSVIC 420
           +HTLILGWSDKLGSLLNQI+IANESLGGGTVVVMAERDKEEMEL+IAKMEFDFKGTSVIC
Sbjct: 361 NHTLILGWSDKLGSLLNQISIANESLGGGTVVVMAERDKEEMELDIAKMEFDFKGTSVIC 420

Query: 421 RSGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL 480
           RSGSPLILADLKKVSVSKARAI+VIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL
Sbjct: 421 RSGSPLILADLKKVSVSKARAIVVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL 480

Query: 481 SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540
           SDLDNE+LVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP
Sbjct: 481 SDLDNELLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540

Query: 541 QLDGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA 600
           QL+GMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA
Sbjct: 541 QLNGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA 600

Query: 601 ALPTVREASFMHIARPTRKPQKILLCGWRRDIDDMIV------------------VWRGS 660
           ALPTV E SF+HIARPTRKPQKILLCGWRRDIDDMIV                  VWRGS
Sbjct: 601 ALPTVGETSFIHIARPTRKPQKILLCGWRRDIDDMIVVSAFIKALILSFWHWMLQVWRGS 660

Query: 661 LPKDFIVPKSTEKILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLIDGGL 720
           LPKDFIVPKS E+ILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKL+DGGL
Sbjct: 661 LPKDFIVPKSAERILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLVDGGL 720

Query: 721 DISRLENISLVDREGNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLI 780
           DISRLENISLVDREGNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLI
Sbjct: 721 DISRLENISLVDREGNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLI 780

Query: 781 RDIQGRPRSHVMTLALSTDEDGASMLREEDRAMTMTEEDVITMLVVTLMMSMDVVVIRAK 840
           RDIQ                                                      AK
Sbjct: 781 RDIQ------------------------------------------------------AK 840

Query: 841 RLPVRDAKIVGYRGSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNE 900
           R+PVR AK V YRGS SQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNE
Sbjct: 841 RMPVRGAKTVTYRGSVSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNE 900

Query: 901 LVSMALAMVAEDRQINDVLEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRREV 960
           LVSMALAMVAEDRQINDVLEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRRE+
Sbjct: 901 LVSMALAMVAEDRQINDVLEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRREI 941

Query: 961 VIGYRSANAERAVINPPAKNERRKWSLGDVFVVIAEKE 981
           VIGYRSA+AERAVINPPAK+ERRKW LGDVFVVIAEKE
Sbjct: 961 VIGYRSAHAERAVINPPAKHERRKWYLGDVFVVIAEKE 941

BLAST of Spg025803 vs. ExPASy TrEMBL
Match: A0A1S3BE18 (ion channel CASTOR-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488869 PE=3 SV=1)

HSP 1 Score: 1552.3 bits (4018), Expect = 0.0e+00
Identity = 829/980 (84.59%), Postives = 863/980 (88.06%), Query Frame = 0

Query: 1   MSLDSESSPSSSRDWFFPPQSFIHSYPAKSQKYIRRFSETSRISQRYTDYQRYRKSSSCI 60
           MSLDSESSPSSSRDWFFPPQSF+HS+PAKS  YIRRFS+TSR+S+RYTDY RYRK+SSCI
Sbjct: 1   MSLDSESSPSSSRDWFFPPQSFLHSHPAKSPNYIRRFSDTSRLSRRYTDYHRYRKTSSCI 60

Query: 61  SDSHSCSTTSNDVKFARTRRRFDFDRRSDLSLKRSELDSPSKQKLELPDVSSSAKKVSDS 120
           SDSHSCST +NDVKFAR+RRRF FDRRSDLSLKRSE++  SK KLELPDVSSSAKKVSD+
Sbjct: 61  SDSHSCSTITNDVKFARSRRRFYFDRRSDLSLKRSEVEFSSKPKLELPDVSSSAKKVSDT 120

Query: 121 ARLFRSLDSTLKVRWHFLAIAVSILVVIFATLVHENLSLQEQVNDLETRISNLYVKLRAC 180
           +RL +S DS+LKVRW FLAIA SI VV FAT+VHENLSLQEQVN+LETRISNL +KLRAC
Sbjct: 121 SRLSKSFDSSLKVRWLFLAIAASIFVVSFATIVHENLSLQEQVNNLETRISNLNIKLRAC 180

Query: 181 NLLDSGSEDDVRSPDELADVASDKRLKTLALIASLTLLSAPVIILKYIDYISKSRSFDHN 240
           NL D G+EDDVRSPDELADV +DKRLKTLALIASLTLL AP+IILKYIDYISKSRS DHN
Sbjct: 181 NLFDHGNEDDVRSPDELADVVTDKRLKTLALIASLTLLFAPIIILKYIDYISKSRSLDHN 240

Query: 241 LEEVSLSKQLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDCLVDCLWLSW 300
           LEE+SL+K+LAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDD LVDCLWLSW
Sbjct: 241 LEEISLNKRLAYKVDVFFSIHPYAKPLALLIATLLLIMLGGLALFGVTDDNLVDCLWLSW 300

Query: 301 TYVADSGNHANSEGIGPRLVSVSISFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ 360
           TYVADSGNHANSEG GPRLVSVS+SFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ
Sbjct: 301 TYVADSGNHANSEGFGPRLVSVSVSFGGMLIFAMMLGLVSDSISEKFDSLRKGRSEVVEQ 360

Query: 361 DHTLILGWSDKLGSLLNQIAIANESLGGGTVVVMAERDKEEMELEIAKMEFDFKGTSVIC 420
           +HTLILGWSDKLGSLLNQI+IANESLGGG VVVMAERDKEEMEL+IAKMEFDFKGTSVIC
Sbjct: 361 NHTLILGWSDKLGSLLNQISIANESLGGGIVVVMAERDKEEMELDIAKMEFDFKGTSVIC 420

Query: 421 RSGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL 480
           R+GSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL
Sbjct: 421 RTGSPLILADLKKVSVSKARAIIVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEL 480

Query: 481 SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540
           SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP
Sbjct: 481 SDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWP 540

Query: 541 QLDGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPA 600
           QL GMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVL+EGDEVLVIAEDDDTYAPA
Sbjct: 541 QLTGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPEDSYVLEEGDEVLVIAEDDDTYAPA 600

Query: 601 ALPTVREASFMHIARPTRKPQKILLCGWRRDIDDMIVVWRGSLPKDFIVPKSTEKILLCG 660
            LPT                                 VWRGSLPKDFIVPKS E+ILLCG
Sbjct: 601 PLPT---------------------------------VWRGSLPKDFIVPKSAERILLCG 660

Query: 661 WRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLIDGGLDISRLENISLVDREGNAV 720
           WRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKL+DGGLDISRLENISLVDREGNAV
Sbjct: 661 WRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKKLVDGGLDISRLENISLVDREGNAV 720

Query: 721 IRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLIRDIQGRPRSHVMTLALST 780
           IRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLIRDIQ              
Sbjct: 721 IRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLIRDIQ-------------- 780

Query: 781 DEDGASMLREEDRAMTMTEEDVITMLVVTLMMSMDVVVIRAKRLPVRDAKIVGYRGSFSQ 840
                                                   AKR+PVRDAK   ++GSFSQ
Sbjct: 781 ----------------------------------------AKRMPVRDAKATAHKGSFSQ 840

Query: 841 GSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDV 900
           GSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDV
Sbjct: 841 GSWIGEMQQASDKSVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDRQINDV 893

Query: 901 LEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRREVVIGYRSANAERAVINPPA 960
           LEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRREVVIGYRSANAERAVINPPA
Sbjct: 901 LEELFAEEGNELHIRQADLYLREGEELSFYEVLLRARQRREVVIGYRSANAERAVINPPA 893

Query: 961 KNERRKWSLGDVFVVIAEKE 981
           KNERRKWSL DVFVVIAEKE
Sbjct: 961 KNERRKWSLRDVFVVIAEKE 893

BLAST of Spg025803 vs. TAIR 10
Match: AT5G49960.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF1012 (InterPro:IPR010420); BEST Arabidopsis thaliana protein match is: Protein of unknown function (DUF1012) (TAIR:AT5G02940.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 882.9 bits (2280), Expect = 2.4e-256
Identity = 477/765 (62.35%), Postives = 567/765 (74.12%), Query Frame = 0

Query: 221 PVIILKYIDYISKSRSF----DHNLEEVSLSKQLAYKVDVFFSIHPYAKPLALLIATLLL 280
           P ++  Y+D +S  ++     +   E+V L K+LAY +DV FS++PYAK LALL+AT++L
Sbjct: 148 PFLLYMYLDDLSHVKNLLRRTNQKKEDVPLKKRLAYSLDVCFSVYPYAKLLALLLATVVL 207

Query: 281 IMLGGLALFGVTDDCLVDCLWLSWTYVADSGNHANSEGIGPRLVSVSISFGGMLIFAMML 340
           I+ GGLAL+ V+D  + + LWLSWT+VADSG+HA+  G+G R+VSV+IS GGMLIFA ML
Sbjct: 208 IVYGGLALYAVSDCGVDEALWLSWTFVADSGSHADRVGVGARIVSVAISAGGMLIFATML 267

Query: 341 GLVSDSISEKFDSLRKGRSEVVEQDHTLILGWSDKLGSLLNQIAIANESLGGGTVVVMAE 400
           GL+SD+IS+  DSLRKG+SEV+E +H LILGWSDKLGSLL Q+AIAN+S+GGG VVV+AE
Sbjct: 268 GLISDAISKMVDSLRKGKSEVLESNHILILGWSDKLGSLLKQLAIANKSIGGGVVVVLAE 327

Query: 401 RDKEEMELEIAKMEFDFKGTSVICRSGSPLILADLKKVSVSKARAIIVIAEDGNADQSDA 460
           RDKEEME +IAK EFD  GTSVICRSGSPLILADLKKVSVS ARAIIV+  D NADQSDA
Sbjct: 328 RDKEEMETDIAKFEFDLMGTSVICRSGSPLILADLKKVSVSNARAIIVLGSDENADQSDA 387

Query: 461 RALRTVLSLTGVKEGLRGHIVVELSDLDNEVLVKLVGGELVETVVAHDVIGRLMIQCARQ 520
           RALR VLSLTGVKEG +GH+VVE+ DLDNE LVKLVGGE +ETVVAHDVIGRLMIQCA Q
Sbjct: 388 RALRVVLSLTGVKEGWKGHVVVEMCDLDNEPLVKLVGGERIETVVAHDVIGRLMIQCALQ 447

Query: 521 PGLAQIWEDILGFENCEFYIKRWPQLDGMQFEDVLISFPDAIPCGIKVASRGGKIVLNPE 580
           PGLAQIWEDILGFEN EFYIK+WPQLDG  FEDVLISFP+AIPCG+KVA+  GKIVLNP 
Sbjct: 448 PGLAQIWEDILGFENAEFYIKKWPQLDGYCFEDVLISFPNAIPCGVKVAA-DGKIVLNPS 507

Query: 581 DSYVLQEGDEVLVIAEDDDTYAPAALPTVREASFMHIARPTRKPQKILLCGWRRDIDDMI 640
           D YVL+EGDE+LVIAEDDDTYAP +LP VR   F  +  P + P+KIL CGWRRDID   
Sbjct: 508 DDYVLKEGDEILVIAEDDDTYAPGSLPEVRMCHFPKMQDPPKYPEKILFCGWRRDID--- 567

Query: 641 VVWRGSLPKDFIVPKSTEKILLCGWRRDMEDMIMVLDAFLAPGSELWMFNDVPENEREKK 700
                                         DMI VL+A LAPGSELWMFN+VP+ EREKK
Sbjct: 568 ------------------------------DMIKVLEALLAPGSELWMFNEVPDQEREKK 627

Query: 701 LIDGGLDISRLENISLVDREGNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSL 760
           L D GL+IS+L NI LV R+GNAVIRRHLESLPLE+FDSILILA++S+E+S + +DSRSL
Sbjct: 628 LTDAGLNISKLVNIKLVHRQGNAVIRRHLESLPLETFDSILILAEQSLENSIVHSDSRSL 687

Query: 761 ATLLLIRDIQGRPRSHVMTLALSTDEDGASMLREEDRAMTMTEEDVITMLVVTLMMSMDV 820
           ATLLLIRDIQ                                                  
Sbjct: 688 ATLLLIRDIQ-------------------------------------------------- 747

Query: 821 VVIRAKRLPVRDAKIVGYR-GSFSQGSWIGEMQQASDKSVIISEILDPRTKNLLSMSKIS 880
               +KRLP +DAK    R   F    WI +MQQASDKS++ISEILD RTKNL+S+S+IS
Sbjct: 748 ----SKRLPYKDAKSSALRISGFPNCCWIRKMQQASDKSIVISEILDSRTKNLVSVSRIS 807

Query: 881 DYVLSNELVSMALAMVAEDRQINDVLEELFAEEGNELHIRQADLYLREGEELSFYEVLLR 940
           DYVLSNELVSMALAMVAED+QIN VL+ELFAE+GNEL IR A+ Y+ + EE+ FY+++ R
Sbjct: 808 DYVLSNELVSMALAMVAEDKQINRVLKELFAEKGNELCIRPAEFYIYDQEEVCFYDIMRR 824

Query: 941 ARQRREVVIGYRSANAERAVINPPAKNERRKWSLGDVFVVIAEKE 981
           ARQR+E++IGYR A  E+AVINP  K++  KWSL DVFVVIA  +
Sbjct: 868 ARQRQEIIIGYRLAGMEQAVINPTDKSKLTKWSLDDVFVVIASSQ 824

BLAST of Spg025803 vs. TAIR 10
Match: AT5G43745.1 (Protein of unknown function (DUF1012) )

HSP 1 Score: 203.8 bits (517), Expect = 6.5e-52
Identity = 182/726 (25.07%), Postives = 341/726 (46.97%), Query Frame = 0

Query: 267 LALLIATLLLIMLGGLALFGVTDDC-LVDCLWLSWTYVADSGNHANSEGIGPRLVSVSIS 326
           +AL+IA +  +++GGL  F    D  L DCLW +W  +  S  H   +    R++   ++
Sbjct: 167 VALMIACVSFVIIGGLLFFKFRKDLPLEDCLWEAWACLISSSTHLKQKTRIERVIGFVLA 226

Query: 327 FGGMLIFAMMLGLVSDSISEKFDSLRKG-RSEVVEQDHTLILGWSDKLGSLLNQIAIANE 386
             G+L ++ +L  +++        LR+G + +V+E DH +I G +  L  +L Q+   +E
Sbjct: 227 IWGILFYSRLLSTMTEQFRYNMTKLREGAQMQVLEADHIIICGINSHLPFILKQLNSYHE 286

Query: 387 ---SLGGGT-----VVVMAERDKEEMELEIAKMEFDFKGTSVICRSGSPLILADLKKVSV 446
               LG  T     +++M++  +++M+        DF    ++ +S S  +    ++ + 
Sbjct: 287 HAVRLGTATARKQRLLLMSDTPRKQMDKLAEAYSKDFNHIDILTKSCSLNLTKSFERAAA 346

Query: 447 SKARAIIVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVELSDLDNEVLVKLVGGEL 506
           S ARAII++   G+  + D  A  +VL+L  +++      +VE+S  +   L+K + G  
Sbjct: 347 SMARAIIILPTKGDRYEVDTDAFLSVLALQPIQKMESIPTIVEVSSPNTYDLLKSISGLK 406

Query: 507 VETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWPQLDGMQFEDVLISFPD 566
           VE V   +V  +L +QC+RQ  L +I+  +L +    F +  +P L G ++  + + F +
Sbjct: 407 VEPV--ENVTSKLFVQCSRQKDLIKIYRHLLNYSKNVFNLCSFPNLVGTKYRQLRLGFQE 466

Query: 567 AIPCGIKVASRGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPAALPTVREASFMHIARP 626
            + CG+    R GK+  +P D+  L E D++L IA   +      L T  +   + +   
Sbjct: 467 VVVCGL---LRDGKVNFHPNDNEELMETDKLLFIA-PLNWKKKQLLYTDMKLENITVPTD 526

Query: 627 TRKPQKILLCGWRRDIDDMIVVWRGSLPK-DFIVPKSTEKILLCGWRRDMEDMIMVLDAF 686
           TRK    +    R  +  +I+  R SL K    V   TE ILL GWR D+  MI   D +
Sbjct: 527 TRKQ---VFEKKRSRLSKIIMRPRKSLSKGSDSVKGPTESILLLGWRGDVVQMIEEFDNY 586

Query: 687 LAPGSELWMFNDVPENEREKKLIDGGLDISRLENISLVDREGNAVIRRHLESLPLESFDS 746
           L PGS + + +DV   +R +  +   +   +++NI +  + GN          PL ++D+
Sbjct: 587 LGPGSSMEILSDVSLEDRRR--VGDSIGSVKIKNIQVSHKVGN----------PL-NYDT 646

Query: 747 ILILADESVEDSAIQADSRSLATLLLIRDIQGRPRSHVMTLALSTDEDGASMLREEDRAM 806
           +                     T++ ++    + ++  +T+ + +D D   +L +  RA 
Sbjct: 647 L-------------------KQTIMRMKSKYRKGKNIPLTILVISDRDW--LLGDPSRAD 706

Query: 807 TMTEEDVITMLVVTLMMSMDVVVIRAKRLPVRDAKIVGYRGSFSQGSWIGEMQQASDKSV 866
             +   +  +L  ++   + V V                                     
Sbjct: 707 KQSAYSL--LLAESICNKLGVKVHN----------------------------------- 766

Query: 867 IISEILDPRT-KNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFAEEGNELHI 926
           + SEI+D +  K +  +     ++ + E++S+  A VAE+ ++N+V +++   +G+E+++
Sbjct: 767 LASEIVDSKLGKQITGLKPSLTFIAAEEVMSLVTAQVAENSELNEVWKDILDADGDEIYV 810

Query: 927 RQADLYLREGEELSFYEVLLRARQRREVVIGYRSANAERAVINPPAKNERRKWSLGDVFV 981
           +  +LY++EGE  SF E+  RA  RREV IGY      + +INP  KNE     + D  +
Sbjct: 827 KDVELYMKEGENPSFTELSERAWLRREVAIGY--IKGGKKMINPVPKNEPLSLEMDDSLI 810

BLAST of Spg025803 vs. TAIR 10
Match: AT5G02940.1 (Protein of unknown function (DUF1012) )

HSP 1 Score: 196.8 bits (499), Expect = 8.0e-50
Identity = 181/729 (24.83%), Postives = 336/729 (46.09%), Query Frame = 0

Query: 267 LALLIATLLLIMLGGLALFGV-TDDCLVDCLWLSWTYVADSGNHANSEGIGPRLVSVSIS 326
           + LLI     +++GGL  F    D  L DCLW +W  + ++  H   +    RL+   ++
Sbjct: 163 VVLLITCFSFVIIGGLFFFKFRKDTSLEDCLWEAWACLVNADTHLEQKTRFERLIGFVLA 222

Query: 327 FGGMLIFAMMLGLVSDSISEKFDSLRKG-RSEVVEQDHTLILGWSDKLGSLLNQ------ 386
             G++ ++ +L  +++        +R+G   +V+E DH +I G +  L  +L Q      
Sbjct: 223 IWGIVFYSRLLSTMTEQFRYHMKKVREGAHMQVLESDHIIICGINSHLPFILKQLNSYQQ 282

Query: 387 --IAIANESLGGGTVVVMAERDKEEMELEIAKMEFDFKGTSVICRSGSPLILADLKKVSV 446
             + +   +    T+++M++  ++EM+        DF    ++ +S S  +    ++ + 
Sbjct: 283 HAVRLGTTTARKQTLLLMSDTPRKEMDKLAEAYAKDFDQLDILTKSCSLNMTKSFERAAA 342

Query: 447 SKARAIIVIAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVELSDLDNEVLVKLVGGEL 506
             ARAII++   G+  + D  A  +VL+L  +++      +VE+S  +   L+K + G  
Sbjct: 343 CMARAIIILPTKGDRYEVDTDAFLSVLALEPIQKMESIPTIVEVSSSNMYDLLKSISGLK 402

Query: 507 VETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWPQLDGMQFEDVLISFPD 566
           VE V   +   +L +QC+RQ  L +I+  +L +    F +  +P L GM++  + + F +
Sbjct: 403 VEPV--ENSTSKLFVQCSRQKDLIKIYRHLLNYSKNVFNLCSFPNLTGMKYRQLRLGFQE 462

Query: 567 AIPCGIKVASRGGKIVLNPEDSYVLQEGDEVLVIAEDDDTYAPAALPTVREASFMHIARP 626
            + CGI    R GK+  +P D   L E D++L IA     +    + T            
Sbjct: 463 VVVCGI---LRDGKVNFHPNDDEELMETDKLLFIAPLKKDFLYTDMKTENMT-----VDE 522

Query: 627 TRKPQKILLCGWRRDIDDMIVVWRGSLPK---DFIVPKSTEKILLCGWRRDMEDMIMVLD 686
           T   +K +    +  ++ +I     SL K    F  PK  E ILL GWR D+ +MI   D
Sbjct: 523 TDDTRKQVYEEKKSRLEKIITRPSKSLSKGSDSFKGPK--ESILLLGWRGDVVNMIKEFD 582

Query: 687 AFLAPGSELWMFNDVPENEREKKLIDGGLDISRLENISLVDREGNAVIRRHLESLPLESF 746
           ++L PGS L + +DVP  +R  + +D  +   +++NI +    GN     H++   L+  
Sbjct: 583 SYLGPGSSLEILSDVPLEDR--RGVDQSIATGKIKNIQVSHSVGN-----HMDYDTLK-- 642

Query: 747 DSILILADESVEDSAIQADSRSLATLLLIRDIQGRPRSHVMTLALSTDEDGASMLREEDR 806
           +SI+ + ++  +                  DI+       +T+ + +D D   +L +  R
Sbjct: 643 ESIMHMQNKYEKGE---------------EDIR-------LTIVVISDRD--LLLGDPSR 702

Query: 807 AMTMTEEDVITMLVV-TLMMSMDVVVIRAKRLPVRDAKIVGYRGSFSQGSWIGEMQQASD 866
           A    ++   T+L+  T+   + V V                                  
Sbjct: 703 A---DKQSAYTLLLAETICNKLGVKVHN-------------------------------- 762

Query: 867 KSVIISEILDPRT-KNLLSMSKISDYVLSNELVSMALAMVAEDRQINDVLEELFAEEGNE 926
              + SEI+D +  K +  +     ++ + E++S+  A VAE+ ++N+V +++   EG+E
Sbjct: 763 ---LASEIVDTKLGKQITRLKPSLTFIAAEEVMSLVTAQVAENSELNEVWKDILDAEGDE 806

Query: 927 LHIRQADLYLREGEELSFYEVLLRARQRREVVIGYRSANAERAVINPPAKNERRKWSLGD 981
           ++++  +LY++EGE  SF E+  RA  RREV IGY      + +INP  K E     + D
Sbjct: 823 IYVKDIELYMKEGENPSFTELSERAWLRREVAIGY--IKGGKKIINPVPKTEPVSLEMED 806

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0034178.10.0e+0087.86ion channel CASTOR-like isoform X2 [Cucumis melo var. makuwa][more]
TYK15741.10.0e+0086.79ion channel CASTOR-like isoform X2 [Cucumis melo var. makuwa][more]
XP_022956408.10.0e+0085.87ion channel CASTOR-like isoform X1 [Cucurbita moschata] >XP_022956409.1 ion chan... [more]
XP_022997206.10.0e+0085.97ion channel CASTOR-like isoform X1 [Cucurbita maxima] >XP_022997214.1 ion channe... [more]
KAG6601611.10.0e+0085.87Ion channel CASTOR, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q5H8A60.0e+0066.16Ion channel CASTOR OS=Lotus japonicus OX=34305 GN=CASTOR PE=1 SV=1[more]
Q75LD50.0e+0064.47Probable ion channel CASTOR OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g084... [more]
Q6RHR61.0e-27562.11Ion channel DMI1 OS=Medicago truncatula OX=3880 GN=DMI1 PE=1 SV=1[more]
Q4VY514.3e-27465.47Probable ion channel SYM8 OS=Pisum sativum OX=3888 GN=SYM8 PE=1 SV=3[more]
Q5N9411.8e-27265.12Probable ion channel POLLUX OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g087... [more]
Match NameE-valueIdentityDescription
A0A5A7SU610.0e+0087.86Ion channel CASTOR-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A5D3CV000.0e+0086.79Ion channel CASTOR-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A6J1GWH30.0e+0085.87ion channel CASTOR-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458157... [more]
A0A6J1K6V90.0e+0085.97ion channel CASTOR-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492175 P... [more]
A0A1S3BE180.0e+0084.59ion channel CASTOR-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488869 PE=3 ... [more]
Match NameE-valueIdentityDescription
AT5G49960.12.4e-25662.35unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF1012... [more]
AT5G43745.16.5e-5225.07Protein of unknown function (DUF1012) [more]
AT5G02940.18.0e-5024.83Protein of unknown function (DUF1012) [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 392..412
NoneNo IPR availableCOILSCoilCoilcoord: 156..176
NoneNo IPR availableGENE3D3.40.50.720coord: 352..497
e-value: 7.9E-16
score: 60.0
NoneNo IPR availablePANTHERPTHR31563:SF1ION CHANNEL CASTOR-RELATEDcoord: 3..638
NoneNo IPR availablePANTHERPTHR31563:SF1ION CHANNEL CASTOR-RELATEDcoord: 809..980
coord: 638..772
IPR010420CASTOR/POLLUX/SYM8 ion channel, conserved domainPFAMPF06241Castor_Poll_midcoord: 503..600
e-value: 9.3E-44
score: 147.7
IPR044849Ion channel CASTOR/POLLUX/SYM8-likePANTHERPTHR31563ION CHANNEL POLLUX-RELATEDcoord: 638..772
IPR044849Ion channel CASTOR/POLLUX/SYM8-likePANTHERPTHR31563ION CHANNEL POLLUX-RELATEDcoord: 809..980
IPR044849Ion channel CASTOR/POLLUX/SYM8-likePANTHERPTHR31563ION CHANNEL POLLUX-RELATEDcoord: 3..638

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg025803.1Spg025803.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006813 potassium ion transport
biological_process GO:0006811 ion transport
cellular_component GO:0016021 integral component of membrane