Spg025704 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg025704
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Proteasome subunit beta
Location	scaffold13: 31783673 .. 31789653 (+)
RNA-Seq Expression	Spg025704
Synteny	Spg025704

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCTTAACATGGATCTCAATGCTCCTCACTCCATGGGTACCACCATCATCGGCGTCACTTACGACGGCGGCGTCGTCCTCGGTGCCGATTCTCGTACCAGCACTGGTGAGTTTATCGCATTCACTTTCTAGCCCTGGTTTCTTAGTATGCTAATCCTTTTTTTTTTTTTCTATGAATATTATATTCAAGAGATGTCTGAAATGATTAAATGCAAGTGGAGGAAAAGTAGTAAGAATTTGTTGGTCTGTCGACCTTGGAAGTAAATCAATTTGTACCAATCGCATTCGGAATTAAGCTCAACTTAGTTGTTTCGAGTTTTCATGAGTAGGTGCAATCATTGAAACAATTCATTTCTCTTATAGTTTTTATCCCCCTAATTAGCATCCAGCGACAGTGATTGGGTGGATTCTGTTGTTGGTTGGATCCTCTTTATGTAAATCCTCTGCAATTTTTAGAACATTACATGACTGAAATTCAGGCTGGCCTTTTCAAGCTTAGCTGCCTAAATCGACAAGCTACGAGCTTTATTAAATACTAACTTCTTGCTTCAGCGACGGGTGCAGAAAACTTATGTAATGCAGTGGAGGGGATAAACGAACTAAATTCTGGGAAAAAGTAACTTGTAAACAAACCAACGCTACCACATTATGTTTACATTAGTTTTTTGTTTTTGGAAACAGAAAAGAATTTTAAAACTCAAATCCCAAGCATTCTTAAATAACAAAATTACATATTAAATGGAAAAATGGATCTTAGTAGGTTTGTTTGTATCCACCTTTTTCTTATGTGTTTGCTTTCTGGTGCGGCATTGACATTGACATATATTTTTTTATTGTTCTTTTGATCTTTTGATAGGTGTCTATGTTGCTAATCGTGCATCAGACAAGATTACAAAGCTTACTGACAATGTCTATGTTTGTCGGTCTGGCTCGGTATTTCCATTTCTGTTATTTCCCATATTAAACTGTCTCAGTCAGATGTTGGTAGTGAAAAATACTTCAAGTAGTTTTTGCTTTGCTTGGGTTTAGTGTTTCCTTCCAGTCTTCAATTCTCAAGTTTTTTCTTTCAACTTCTTGTTCTATCATTAAGATCTCAAGAAATCTTTCAGTTCATGGGTGTTTGCTCTTCTTTCATAAATGGTTTATTTTGCTTCTCTTGCAGGCTGCAGATTCTCAAATTGTCTCTGATTATGTGCGTCACTTCCTTCATCAGCATACGTGAGTGCTTTGATTATTGACAATATTATGTTGCAACTTTGAATTTGTTGGTTAATCATAAGTTGATCACTTTGAGTATTTTTCTTCTATTGCTTGAATGTTTGTGATTGCTTTTGCAAGATTGGATCTGCTGGCAACTGCATTTCTTTTCATCTGTTTTCCCAGTGGTTTTCAAAACCGTATGAAAGAGTTGAAAATTCTATATTTTATTTCCGTGTAATAAAAATAATTTGACCCATAAATCAATGACATGTTGTTGGGAGAAGAAGGGTGATGACTTAGGCCCCGTTTGATAACCATTTGGTTTTTTGTTTTCAAAAATTAAGCTTACAAAGACTACCACCGCCTATAAGTCTTTTTGTTTTGTTATCAATCTTCTTCCTTTGTTTGCAAAAACTTAGTCGAAGTTTTGAAAACTAAAAAAAAATGTAGTTTTCAAAATCTTGTTTTTTGTTTTTGTAATTTTGTTGAGAATTCAAGTGTGTCCTTAACAAAGATGAAAACAATGGTAAGGAAATTGGGAGAAAATGAGCCTAATTTTAAAAAACCAAATAGTTTCAAATGGGGCCTTAATTTTTTCTTTACATAATACGATAACTAGCCACTATGTTAACTAGCTCGATGAGGAAAATGTCCGGTGCATACGCCATGCTGTCCCAGTGATTGATGTTATAGCACTAGATATGCATGATCTGGTGGGAATGTGTAATTTGGGATTCATTAAAGATGGTGTTTATTTAGAATTTCTCTAGGTAAATGTACCTAAACATCCTCTTTCTTTTTTTCAAATGATAAGTGATCTCAAAATATGTTAAAATTTTGGTTGTATGATCTAGCTGGAGATATGTTATTGGTAATTGTAATACTGTTTTCTTAATCCTAAATTGCTTTTACTACCTCCGTTTCATTATTGTCTCTTTGCTGATAAGTCAACCTTAAGATAATTGTAACTCATGTCTTATTGCTGATTGTATATTACCTTTGATGCACAGGATACAGCTTGGCCAGCCTGCAACTGTTAAAGTGGCTGCAAACCTTGTTAGGCTATTAGCATATAACAACAAGGTAGGAGCTCATATTAGTTTGTTCAAAATTATTGCATGTTAAATATGTATGTATAGCAATTTTGGATAATAACAATAACAGTTCATTGAAACAGTGCAAATCCCCCTACCCAGCCAAGGAGTTAGGAAAAAAATAATCTCCAAGTGGCCAAATCATGGAAATAGACTAATCACACAAGTGCCTAGCTAGACAAGAGAAACCTCTTCTCCACAGAAAATCCTATTGTTTTTTTTTTCCCGCAACCAGGGCTCCTAGAAAACCACGATGATATTGATCCACAGGACCCTTGTCTTTTCTCTGAATGGGTGACTGCCGTAGGGCCTGTTGAAGATCCACAGGATCCCTTCTCTTTTCTAGGAGCTCATATTAGTTTGTTCAAAATTATTGCATGTTACATATGTATGTATAGCAATTTTGGATAAGAACAATAACAAACAATTCATCGAAACAGTGCAAATGCCCCAACCTAGCCAAGGAGTTAGAAAAAAAATTCTCCAAGTGGCCAAATCATGGAAATAGACTAATCACAAGTGCCTAGCTAGACAAGAGAAACCTCTTATCCATAGAAAATCCTATTGTTTTCTTTCTTTTTCTTTCTTTCTTTCTTCTTCTTCTTTTTTTTCCTGCAACCAGGGCTCCCAGAAAACCATAATGATATTGATCCACAGGACCCTTCTCTTTTCTTTAAATGGGTGACTGCCGCAGGGCCTGTTGAAGATCCTCAACAACCCCCCACCCAATCCCCAACCCCTAAGAAGTAATTCTATTAAGTCAAAATGTGAGTTCAATAAGGAGGGAGGACGGAACATCCCAACTAGCCAGAGTAGATAAAGAAAACATTACAAAATAATTATGTGATTGAGTAGTATATGTATATCATCACAGTTAGTTTTTCTGTTTTTCTGTTCTTCTGTAATGAACATTTCAGAGTTGCATGTTAAAGTAAAACAAGAAAGAGAGGAGGGAAAATGAATTAGGTAAAATATAAATAAGGTATGGACTTGTGACATTTGAACTTTTTTTTTTTTAAAAAAATAAATATGAGAAACAGATGCAGTAAGCTGTACCACTCCAAAAAGCCCCAAAAGAGGAGGACTTATCATCAAAGGTTCTCTGATTCCTCTCCAAACGTATGTAGAAACAATCTCCAGAACCATCAAATGATATTTACTAAACAAGAGGGTGTCATCTGCATATTGAATGTAGGTGACACCTCCTCATCTCCAACCTTGAAGCTCTCGATGTTTTTCTTTTCATTGCAAGCATCAATAAAATGACTAAAGGCATCTATCACTATGGTGAAAAGGAATGGGGACAACAAATCTCCTTGTCTCAAACCTCTCCATTAATCATAATAGAGAAGTTTGCAGAGAAAAGACACCCAAGGATCCACTTTCTTCATCTTTTATTAAGCTTTTTTTTCCTCCATGATTCTAGGAAAGACCCATTGACTTTATCATATGCCTTCTCAAAGTCTAGTTTAATGACGAAAACTTCTTCTTACTCGTGTAGTCATAAACTGTTTTGGCCACTAAGAGGATGGCATCAATGATTTGTCTATCCTCTATGAACGCCATTTAGGTTTCATTTATGATTGAAGGTAAGATTTTCAGAGTCTTTTGGCCAACACTTTTCCAATAACCTTATACAAAGCAGAGACGAGGCTAATGGGTTTGTAATAAATAACCCACCGTTCGTGCATCTAACTTCTTAGGGATCAAGCAAATGTAGGTTTCCTTTGTTTTCCTATTGATGATGCCTTTTTCAAGAAACTCTTGGAATGCCCTGAGCAAATCAACTTTGATGATGTTTCAACCCTTTTTTTTTCCAAAAACTCAGCATTGAAGCTGTCTGTACTTGGGAATTTAAGATTTACCCATGGCATAAATAGCCGCACAGATTTCTGACTTGTGAGACTGCCCAATGTGAACTCGACTTTTAAGTTTTCTTGAAAATATTTGACCTTCTCATAAAAATATGCTGTATTTTGTTTATTATGGGTTGTGTAAGAACTCAACGTAGGGGAAGAATAGTAATCCTTTGGGATTTTATACTGAGTCTCCTCCTGTTTGTTTGTTTGTTTGGTTTTTTTTTCCCTTCTTCTTCTAACTTTTATCTTAATTCTTATCCATTCAAACAACCCCTAACCAAAACCGACTGATTCACAATAAACTTCTCCATTTTTGTTGGAAAGTTGCTTGACTGACTTTGATATCTTTCTCTTTTAAAATTGTGGCCAAAGATACCCTTCCTTTGTTTTAATGTAGTTCAGGTGGGATGTATGGGGAAAGAGGGAGGAGGGGGTCTCTGTTTCTCTCATCTAAACCAATTTTATATGTGAGAGAGATGAGGTCACCCCCACGTACTCTAATAAAATGCTCACGAGAGTTCTATTTGCTATGTTTAAGTGAAGCTATATTCTTGTTCTTTATTCAAACTAAATAACATGCTTTAATATCAGTGCCATTGGGTTGAATGGACAGGATATGTTACAAACTGGCCTTATTGTTGGTGGATGGGACAAGTACGAAGGTGGTCGAATCTATGGTATTCCTCTTGGTGGAACTATAGTAGAGCAGCCATTTGCTATTGGAGGTAAAACCTTTTGTGCCCAATCCATCATGAAAAATAATGACGTTTGGTGATTTTTTTTTCTGGCTTCCACATGTTTTCATACTCCTGTTTTTCTATGCCAGCTTTATCAGAGGATCTTTTGCTTTATCATCCTTGAATGAATGAGTAGTATTTAGGTTTATATGATCCTGTATCAGGTTTTAGTTATACTGAACCCATAGAGATAGGATTCTCAAGCATAGAAGTCTTCTATGTCAAGGAAATATCTCCCCCTCTTTTCTTTTGGTAGTTAGATGTCTGGCATTTGCTGTTATAATCCTTTGTTCTCTGCTTGCGAGTATGACCATTACGTGGTTCCACTTATTATTTTACAGGATCTGGGTCCAGTTACTTGTATGGATTTTTTGATCAAGCATGGAGAGAAGGAATGACCAGGGAAGAAGCTGAGGTATTTGACTACTCTATAGTCTTATTTGCAAACTAAGGGTTCTGCATTATTGTTGCAGTTACTTATAGTGAGTTTAGGATGACTTCCAAGAAGCGCAATACTTCTTTTCTAAGCACCAAGTAAGATGGAGTTTGCTAAATGCAACCTCTTAGGCAAAAGATTCAATTCTAAGCCATCGATCAATCAATGAAAATACACACACATACCAGCTAAAGTAAATCAGCTGGTAAAATTCAGTTTCGAGGTGGTTGAAGCTTTGGGATGAGCTTCGTTTTCTTTCTATTTGTCCTCAGTGCAAGAAGCTATATCGTTTGAACTTTATTCATGAGATTCTTTTTTCTGATTATATACTATTATTTGCAGCAATTAGTGGTTAAGGCCGTTTCTCTTGCCATTGCCCGAGATGGTGCTAGTGGTGGTGTTGTCCGTACAGTTACTGTAAGTTAATGTCCTTTGATCACATAATCTATTACTTTAAAACATGTCCAATCTTCGGTCTTCATAGTCAAGAACCGTTGCATAAACTCGGGCATTTTTGCAGATAAATTCTGAGGGTGTGACAAGAAAATTCTATCCTGGGGATCAGCTACCACTATGGCACGAGGAATTAGAGCCTAAAAATTCATTGCTGGATGTTCTGAATGCTTCGAGTCCTGAACCGATGAACATATAA

mRNA sequence

ATGGATCTTAACATGGATCTCAATGCTCCTCACTCCATGGGTACCACCATCATCGGCGTCACTTACGACGGCGGCGTCGTCCTCGGTGCCGATTCTCGTACCAGCACTGGTGTCTATGTTGCTAATCGTGCATCAGACAAGATTACAAAGCTTACTGACAATGTCTATGTTTGTCGGTCTGGCTCGGCTGCAGATTCTCAAATTGTCTCTGATTATGTGCGTCACTTCCTTCATCAGCATACGATACAGCTTGGCCAGCCTGCAACTGTTAAAGTGGCTGCAAACCTTGTTAGGCTATTAGCATATAACAACAAGGACCCTTGTCTTTTCTCTGAATGGGTGACTGCCGTAGGGCCTGTTGAAGATCCACAGGATCCCTTCTCTTTTCTAGGAGCTCATATTAGTTTGTTCAAAATTATTGCATGTTACATATGGCTCCCAGAAAACCATAATGATATTGATCCACAGGACCCTTCTCTTTTCTTTAAATGGGTGACTGCCGCAGGGCCTGTTGAAGATCCTCAACAACCCCCCACCCAATCCCCAACCCCTAAGAATGCCATTGGGTTGAATGGACAGGATATGTTACAAACTGGCCTTATTGTTGGTGGATGGGACAAGTACGAAGGTGGTCGAATCTATGGTATTCCTCTTGGTGGAACTATAGTAGAGCAGCCATTTGCTATTGGAGGATCTGGGTCCAGTTACTTGTATGGATTTTTTGATCAAGCATGGAGAGAAGGAATGACCAGGGAAGAAGCTGAGCAATTAGTGGTTAAGGCCGTTTCTCTTGCCATTGCCCGAGATGGTGCTAGTGGTGGTGTTGTCCGTACAGTTACTATAAATTCTGAGGGTGTGACAAGAAAATTCTATCCTGGGGATCAGCTACCACTATGGCACGAGGAATTAGAGCCTAAAAATTCATTGCTGGATGTTCTGAATGCTTCGAGTCCTGAACCGATGAACATATAA

Coding sequence (CDS)

ATGGATCTTAACATGGATCTCAATGCTCCTCACTCCATGGGTACCACCATCATCGGCGTCACTTACGACGGCGGCGTCGTCCTCGGTGCCGATTCTCGTACCAGCACTGGTGTCTATGTTGCTAATCGTGCATCAGACAAGATTACAAAGCTTACTGACAATGTCTATGTTTGTCGGTCTGGCTCGGCTGCAGATTCTCAAATTGTCTCTGATTATGTGCGTCACTTCCTTCATCAGCATACGATACAGCTTGGCCAGCCTGCAACTGTTAAAGTGGCTGCAAACCTTGTTAGGCTATTAGCATATAACAACAAGGACCCTTGTCTTTTCTCTGAATGGGTGACTGCCGTAGGGCCTGTTGAAGATCCACAGGATCCCTTCTCTTTTCTAGGAGCTCATATTAGTTTGTTCAAAATTATTGCATGTTACATATGGCTCCCAGAAAACCATAATGATATTGATCCACAGGACCCTTCTCTTTTCTTTAAATGGGTGACTGCCGCAGGGCCTGTTGAAGATCCTCAACAACCCCCCACCCAATCCCCAACCCCTAAGAATGCCATTGGGTTGAATGGACAGGATATGTTACAAACTGGCCTTATTGTTGGTGGATGGGACAAGTACGAAGGTGGTCGAATCTATGGTATTCCTCTTGGTGGAACTATAGTAGAGCAGCCATTTGCTATTGGAGGATCTGGGTCCAGTTACTTGTATGGATTTTTTGATCAAGCATGGAGAGAAGGAATGACCAGGGAAGAAGCTGAGCAATTAGTGGTTAAGGCCGTTTCTCTTGCCATTGCCCGAGATGGTGCTAGTGGTGGTGTTGTCCGTACAGTTACTATAAATTCTGAGGGTGTGACAAGAAAATTCTATCCTGGGGATCAGCTACCACTATGGCACGAGGAATTAGAGCCTAAAAATTCATTGCTGGATGTTCTGAATGCTTCGAGTCCTGAACCGATGAACATATAA

Protein sequence

MDLNMDLNAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRSGSAADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNKDPCLFSEWVTAVGPVEDPQDPFSFLGAHISLFKIIACYIWLPENHNDIDPQDPSLFFKWVTAAGPVEDPQQPPTQSPTPKNAIGLNGQDMLQTGLIVGGWDKYEGGRIYGIPLGGTIVEQPFAIGGSGSSYLYGFFDQAWREGMTREEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKFYPGDQLPLWHEELEPKNSLLDVLNASSPEPMNI

Homology

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BLAST of Spg025704 vs. NCBI nr
Match: XP_038883996.1 (proteasome subunit beta type-6 [Benincasa hispida])

HSP 1 Score: 432.6 bits (1111), Expect = 3.1e-117
Identity = 232/323 (71.83%), Postives = 235/323 (72.76%), Query Frame = 0


Query: 1   MDLNMDLNAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRS 60
           MDLNMDLNAPHSMGTTIIGVTYDGGV+LGADSRTSTGVYVANRASDKITKLTDNVYVCRS
Sbjct: 1   MDLNMDLNAPHSMGTTIIGVTYDGGVILGADSRTSTGVYVANRASDKITKLTDNVYVCRS 60

Query: 61  GSAADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNKDPCLFSEWVTAVGPV 120
           GSAADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNK               
Sbjct: 61  GSAADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNK--------------- 120

Query: 121 EDPQDPFSFLGAHISLFKIIACYIWLPENHNDIDPQDPSLFFKWVTAAGPVEDPQQPPTQ 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 SPTPKNAIGLNGQDMLQTGLIVGGWDKYEGGRIYGIPLGGTIVEQPFAIGGSGSSYLYGF 240
                        DMLQTGLIVGGWDKYEGGRIYGIPLGGTI+EQPFAIGGSGSSYLYGF
Sbjct: 181 -------------DMLQTGLIVGGWDKYEGGRIYGIPLGGTIIEQPFAIGGSGSSYLYGF 235

Query: 241 FDQAWREGMTREEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKFYPGDQLPLWH 300
           FDQAW+EGMTREEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKFYPGDQLPLWH
Sbjct: 241 FDQAWKEGMTREEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKFYPGDQLPLWH 235

Query: 301 EELEPKNSLLDVLNASSPEPMNI 324
           EELEPKNSLLDVLNASSPEPMNI
Sbjct: 301 EELEPKNSLLDVLNASSPEPMNI 235

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BLAST of Spg025704 vs. NCBI nr
Match: XP_022962546.1 (proteasome subunit beta type-6-like [Cucurbita moschata] >XP_023545809.1 proteasome subunit beta type-6-like [Cucurbita pepo subsp. pepo] >KAG7029736.1 Proteasome subunit beta type-6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 431.8 bits (1109), Expect = 5.2e-117
Identity = 232/323 (71.83%), Postives = 234/323 (72.45%), Query Frame = 0


Query: 1   MDLNMDLNAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRS 60
           MDLNMDLNAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRS
Sbjct: 1   MDLNMDLNAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRS 60

Query: 61  GSAADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNKDPCLFSEWVTAVGPV 120
           GSAADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNK               
Sbjct: 61  GSAADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNK--------------- 120

Query: 121 EDPQDPFSFLGAHISLFKIIACYIWLPENHNDIDPQDPSLFFKWVTAAGPVEDPQQPPTQ 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 SPTPKNAIGLNGQDMLQTGLIVGGWDKYEGGRIYGIPLGGTIVEQPFAIGGSGSSYLYGF 240
                        DMLQTGLIVGGWDKY GGRIYGIPLGGTI+EQPFAIGGSGSSYLYGF
Sbjct: 181 -------------DMLQTGLIVGGWDKYNGGRIYGIPLGGTIIEQPFAIGGSGSSYLYGF 235

Query: 241 FDQAWREGMTREEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKFYPGDQLPLWH 300
           FDQAWREGMTREEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKFYPGDQLPLWH
Sbjct: 241 FDQAWREGMTREEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKFYPGDQLPLWH 235

Query: 301 EELEPKNSLLDVLNASSPEPMNI 324
           EELEPKNSLLDVLNASSPEPMN+
Sbjct: 301 EELEPKNSLLDVLNASSPEPMNV 235

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BLAST of Spg025704 vs. NCBI nr
Match: XP_022996805.1 (proteasome subunit beta type-6-like [Cucurbita maxima])

HSP 1 Score: 429.9 bits (1104), Expect = 2.0e-116
Identity = 231/323 (71.52%), Postives = 233/323 (72.14%), Query Frame = 0


Query: 1   MDLNMDLNAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRS 60
           MDLNMDLNAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRS
Sbjct: 1   MDLNMDLNAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRS 60

Query: 61  GSAADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNKDPCLFSEWVTAVGPV 120
           GSAADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNK               
Sbjct: 61  GSAADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNK--------------- 120

Query: 121 EDPQDPFSFLGAHISLFKIIACYIWLPENHNDIDPQDPSLFFKWVTAAGPVEDPQQPPTQ 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 SPTPKNAIGLNGQDMLQTGLIVGGWDKYEGGRIYGIPLGGTIVEQPFAIGGSGSSYLYGF 240
                        DMLQTGLIVGGWDKY GGRIYGIPLGGTI+EQPFAIGGSGSSYLYGF
Sbjct: 181 -------------DMLQTGLIVGGWDKYNGGRIYGIPLGGTIIEQPFAIGGSGSSYLYGF 235

Query: 241 FDQAWREGMTREEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKFYPGDQLPLWH 300
           FDQAWREGMTREEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGV RKFYPGDQLPLWH
Sbjct: 241 FDQAWREGMTREEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVARKFYPGDQLPLWH 235

Query: 301 EELEPKNSLLDVLNASSPEPMNI 324
           EELEPKNSLLDVLNASSPEPMN+
Sbjct: 301 EELEPKNSLLDVLNASSPEPMNV 235

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BLAST of Spg025704 vs. NCBI nr
Match: XP_022132123.1 (proteasome subunit beta type-6 [Momordica charantia])

HSP 1 Score: 428.7 bits (1101), Expect = 4.4e-116
Identity = 230/323 (71.21%), Postives = 234/323 (72.45%), Query Frame = 0


Query: 1   MDLNMDLNAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRS 60
           MDLNMDLNAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRS
Sbjct: 1   MDLNMDLNAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRS 60

Query: 61  GSAADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNKDPCLFSEWVTAVGPV 120
           GSAADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLL+YNNK               
Sbjct: 61  GSAADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLSYNNK--------------- 120

Query: 121 EDPQDPFSFLGAHISLFKIIACYIWLPENHNDIDPQDPSLFFKWVTAAGPVEDPQQPPTQ 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 SPTPKNAIGLNGQDMLQTGLIVGGWDKYEGGRIYGIPLGGTIVEQPFAIGGSGSSYLYGF 240
                        DMLQTGLIVGGWDKY+GGRIYGIPLGGTI+EQPFAIGGSGSSYLYGF
Sbjct: 181 -------------DMLQTGLIVGGWDKYDGGRIYGIPLGGTIIEQPFAIGGSGSSYLYGF 235

Query: 241 FDQAWREGMTREEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKFYPGDQLPLWH 300
           FDQAWREGM REEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKFYPGDQLPLWH
Sbjct: 241 FDQAWREGMIREEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKFYPGDQLPLWH 235

Query: 301 EELEPKNSLLDVLNASSPEPMNI 324
           EE+EPKNSLLDVLNASSPEPMNI
Sbjct: 301 EEIEPKNSLLDVLNASSPEPMNI 235

[Close]

BLAST of Spg025704 vs. NCBI nr
Match: XP_022951437.1 (proteasome subunit beta type-6 [Cucurbita moschata] >XP_023002488.1 proteasome subunit beta type-6 [Cucurbita maxima] >XP_023537465.1 proteasome subunit beta type-6 [Cucurbita pepo subsp. pepo] >KAG7020325.1 Proteasome subunit beta type-6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 428.3 bits (1100), Expect = 5.8e-116
Identity = 230/323 (71.21%), Postives = 234/323 (72.45%), Query Frame = 0


Query: 1   MDLNMDLNAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRS 60
           MDLNMDLNAPHSMGTTIIGVTY+GGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRS
Sbjct: 1   MDLNMDLNAPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRS 60

Query: 61  GSAADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNKDPCLFSEWVTAVGPV 120
           GSAADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNK               
Sbjct: 61  GSAADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNK--------------- 120

Query: 121 EDPQDPFSFLGAHISLFKIIACYIWLPENHNDIDPQDPSLFFKWVTAAGPVEDPQQPPTQ 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 SPTPKNAIGLNGQDMLQTGLIVGGWDKYEGGRIYGIPLGGTIVEQPFAIGGSGSSYLYGF 240
                        DMLQTGLIVGGWDKYEGGRIYGIPLGGTI+EQPFAIGGSGSSYLYGF
Sbjct: 181 -------------DMLQTGLIVGGWDKYEGGRIYGIPLGGTIIEQPFAIGGSGSSYLYGF 235

Query: 241 FDQAWREGMTREEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKFYPGDQLPLWH 300
           FDQAW+EGMTR+EAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKFYPGDQLPLWH
Sbjct: 241 FDQAWKEGMTRDEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKFYPGDQLPLWH 235

Query: 301 EELEPKNSLLDVLNASSPEPMNI 324
           EELEPKNSLLD LNASSPEPMNI
Sbjct: 301 EELEPKNSLLDALNASSPEPMNI 235

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BLAST of Spg025704 vs. ExPASy Swiss-Prot
Match: Q8LD27 (Proteasome subunit beta type-6 OS=Arabidopsis thaliana OX=3702 GN=PBA1 PE=1 SV=2)

HSP 1 Score: 385.6 bits (989), Expect = 5.6e-106
Identity = 206/321 (64.17%), Postives = 225/321 (70.09%), Query Frame = 0


Query: 1   MDLNMDLNAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRS 60
           MDLN+D  APHSMGTTIIGVTY+GGVVLGADSRTSTG+YVANRASDKIT+LTDNVYVCRS
Sbjct: 1   MDLNLD--APHSMGTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRS 60

Query: 61  GSAADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNKDPCLFSEWVTAVGPV 120
           GSAADSQ+VSDYVR+FLHQHTIQ GQPATVKV+ANL+R+LAYNNK               
Sbjct: 61  GSAADSQVVSDYVRYFLHQHTIQHGQPATVKVSANLIRMLAYNNK--------------- 120

Query: 121 EDPQDPFSFLGAHISLFKIIACYIWLPENHNDIDPQDPSLFFKWVTAAGPVEDPQQPPTQ 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 SPTPKNAIGLNGQDMLQTGLIVGGWDKYEGGRIYGIPLGGTIVEQPFAIGGSGSSYLYGF 240
                        +MLQTGLIVGGWDKYEGG+IYGIPLGGT+VEQPFAIGGSGSSYLYGF
Sbjct: 181 -------------NMLQTGLIVGGWDKYEGGKIYGIPLGGTVVEQPFAIGGSGSSYLYGF 231

Query: 241 FDQAWREGMTREEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKFYPGDQLPLWH 300
           FDQAW++ MT+EEAEQLVVKAVSLAIARDGASGGVVRTV INSEGVTR FYPGD+L LWH
Sbjct: 241 FDQAWKDNMTKEEAEQLVVKAVSLAIARDGASGGVVRTVIINSEGVTRNFYPGDKLQLWH 231

Query: 301 EELEPKNSLLDVLNASSPEPM 322
           EELEP+NSLLD+LNA+ PEPM
Sbjct: 301 EELEPQNSLLDILNAAGPEPM 231

[Close]

BLAST of Spg025704 vs. ExPASy Swiss-Prot
Match: P93395 (Proteasome subunit beta type-6 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 2.4e-101
Identity = 199/318 (62.58%), Postives = 216/318 (67.92%), Query Frame = 0


Query: 4   NMDLNAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRSGSA 63
           N D++ PHSMGTTIIGVTY+GGVVLGADSRTSTG+YVANRASDKIT+LTDNVYVCRSGSA
Sbjct: 3   NTDVDQPHSMGTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRSGSA 62

Query: 64  ADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNKDPCLFSEWVTAVGPVEDP 123
           ADSQIVSDYVR+FLHQHTIQLGQPATVKVAANL RLL+YNNK                  
Sbjct: 63  ADSQIVSDYVRYFLHQHTIQLGQPATVKVAANLTRLLSYNNK------------------ 122

Query: 124 QDPFSFLGAHISLFKIIACYIWLPENHNDIDPQDPSLFFKWVTAAGPVEDPQQPPTQSPT 183
                                                                       
Sbjct: 123 ------------------------------------------------------------ 182

Query: 184 PKNAIGLNGQDMLQTGLIVGGWDKYEGGRIYGIPLGGTIVEQPFAIGGSGSSYLYGFFDQ 243
                     D LQTG+I+GGWDKYEGG+IYGIP GGT++EQPFAIGGSGSSYLYGFFDQ
Sbjct: 183 ----------DRLQTGMIIGGWDKYEGGKIYGIPPGGTVLEQPFAIGGSGSSYLYGFFDQ 232

Query: 244 AWREGMTREEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKFYPGDQLPLWHEEL 303
           AW+EGMT+EEAE+LVV AVSLAIARDGASGGVVRTVTIN +G TRKFY GD L LWHEEL
Sbjct: 243 AWKEGMTQEEAEKLVVTAVSLAIARDGASGGVVRTVTINKDGATRKFYSGDSLQLWHEEL 232

Query: 304 EPKNSLLDVLNASSPEPM 322
           EP NSLLDV+ ASSP PM
Sbjct: 303 EPVNSLLDVVFASSPVPM 232

[Close]

BLAST of Spg025704 vs. ExPASy Swiss-Prot
Match: Q55GJ6 (Proteasome subunit beta type-6 OS=Dictyostelium discoideum OX=44689 GN=psmB6 PE=1 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 8.3e-49
Identity = 107/289 (37.02%), Postives = 152/289 (52.60%), Query Frame = 0


Query: 13  MGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRSGSAADSQIVSDY 72
           +GT+I+ V YDGGV++GADSRT+TG Y+ANR ++KIT + + +Y CRSGSAAD+Q +SDY
Sbjct: 13  LGTSIMAVEYDGGVIMGADSRTTTGAYIANRVTNKITPIHERIYCCRSGSAADTQAISDY 72

Query: 73  VRHFLHQHTIQLGQPATVKVAANLVRLLAYNNKDPCLFSEWVTAVGPVEDPQDPFSFLGA 132
           VR++L  HT +L     VK AA+L +LL Y+NK                           
Sbjct: 73  VRYYLEMHTSELCDEPDVKTAASLFQLLCYSNK--------------------------- 132

Query: 133 HISLFKIIACYIWLPENHNDIDPQDPSLFFKWVTAAGPVEDPQQPPTQSPTPKNAIGLNG 192
                                                                       
Sbjct: 133 ------------------------------------------------------------ 192

Query: 193 QDMLQTGLIVGGWDKYEGGRIYGIPLGGTIVEQPFAIGGSGSSYLYGFFDQAWREGMTRE 252
            + L  G+IV GWDK++GG +Y I LGG++V+QPFAIGGSGS+Y+YG+ D  ++  MT++
Sbjct: 193 -NNLMAGIIVAGWDKHQGGSVYNISLGGSMVKQPFAIGGSGSTYIYGYCDSKFKPKMTKD 213

Query: 253 EAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKFYPGDQLPLWHE 302
           E  + V  +++LA+ RDG+SGGV+R   I+  GV RK  PG+ LP + E
Sbjct: 253 ECIEFVQNSLALAMFRDGSSGGVIRLCIIDKNGVERKMIPGNNLPRFWE 213

[Close]

BLAST of Spg025704 vs. ExPASy Swiss-Prot
Match: Q3MHN0 (Proteasome subunit beta type-6 OS=Bos taurus OX=9913 GN=PSMB6 PE=1 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 4.5e-47
Identity = 107/290 (36.90%), Postives = 145/290 (50.00%), Query Frame = 0


Query: 8   NAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRSGSAADSQ 67
           N   S GTTI+ V +DGGVVLGADSRT+TG Y+ANR +DK+T + D ++ CRSGSAAD+Q
Sbjct: 28  NREVSTGTTIMAVQFDGGVVLGADSRTTTGSYIANRVTDKLTPIHDRIFCCRSGSAADTQ 87

Query: 68  IVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNKDPCLFSEWVTAVGPVEDPQDPF 127
            V+D V + L  H+I+L +P  V  AA+L + + Y  ++                     
Sbjct: 88  AVADAVTYQLGFHSIELNEPPLVHTAASLFKEMCYRYRED-------------------- 147

Query: 128 SFLGAHISLFKIIACYIWLPENHNDIDPQDPSLFFKWVTAAGPVEDPQQPPTQSPTPKNA 187
                                                                       
Sbjct: 148 ------------------------------------------------------------ 207

Query: 188 IGLNGQDMLQTGLIVGGWDKYEGGRIYGIPLGGTIVEQPFAIGGSGSSYLYGFFDQAWRE 247
                   L  G+I+ GWD  EGG++Y +P+GG +V QPFAIGGSGSSY+YG+ D  +RE
Sbjct: 208 --------LMAGIIIAGWDPQEGGQVYSVPMGGMMVRQPFAIGGSGSSYIYGYVDATYRE 229

Query: 248 GMTREEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKFYPGDQLP 298
           GMT+EE  Q    A++LA+ RDG+SGGV+R   I   GV R+   GDQ+P
Sbjct: 268 GMTKEECLQFTANALALAMERDGSSGGVIRLAAIAEPGVERQVLLGDQIP 229

[Close]

BLAST of Spg025704 vs. ExPASy Swiss-Prot
Match: P28073 (Proteasome subunit beta type-6 OS=Rattus norvegicus OX=10116 GN=Psmb6 PE=1 SV=3)

HSP 1 Score: 187.2 bits (474), Expect = 2.9e-46
Identity = 105/290 (36.21%), Postives = 145/290 (50.00%), Query Frame = 0


Query: 8   NAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRSGSAADSQ 67
           N   S GTTI+ V +DGGVVLGADSRT+TG Y+ANR +DK+T + D+++ CRSGSAAD+Q
Sbjct: 27  NREVSTGTTIMAVQFDGGVVLGADSRTTTGSYIANRVTDKLTPIHDHIFCCRSGSAADTQ 86

Query: 68  IVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNKDPCLFSEWVTAVGPVEDPQDPF 127
            V+D V + L  H+I+L +P  V  AA+L + + Y  ++                     
Sbjct: 87  AVADAVTYQLGFHSIELNEPPLVHTAASLFKEMCYRYRED-------------------- 146

Query: 128 SFLGAHISLFKIIACYIWLPENHNDIDPQDPSLFFKWVTAAGPVEDPQQPPTQSPTPKNA 187
                                                                       
Sbjct: 147 ------------------------------------------------------------ 206

Query: 188 IGLNGQDMLQTGLIVGGWDKYEGGRIYGIPLGGTIVEQPFAIGGSGSSYLYGFFDQAWRE 247
                   L  G+I+ GWD  EGG++Y +P+GG +V Q FAIGGSGSSY+YG+ D  +RE
Sbjct: 207 --------LMAGIIIAGWDPQEGGQVYSVPMGGMMVRQSFAIGGSGSSYIYGYVDATYRE 228

Query: 248 GMTREEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKFYPGDQLP 298
           GMT++E  Q    A++LA+ RDG+SGGV+R   I   GV R+   GDQ+P
Sbjct: 267 GMTKDECLQFTANALALAMERDGSSGGVIRLAAIQQSGVERQVLLGDQIP 228

[Close]

BLAST of Spg025704 vs. ExPASy TrEMBL
Match: A0A6J1HDK0 (Proteasome subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111462944 PE=3 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 2.5e-117
Identity = 232/323 (71.83%), Postives = 234/323 (72.45%), Query Frame = 0


Query: 1   MDLNMDLNAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRS 60
           MDLNMDLNAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRS
Sbjct: 1   MDLNMDLNAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRS 60

Query: 61  GSAADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNKDPCLFSEWVTAVGPV 120
           GSAADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNK               
Sbjct: 61  GSAADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNK--------------- 120

Query: 121 EDPQDPFSFLGAHISLFKIIACYIWLPENHNDIDPQDPSLFFKWVTAAGPVEDPQQPPTQ 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 SPTPKNAIGLNGQDMLQTGLIVGGWDKYEGGRIYGIPLGGTIVEQPFAIGGSGSSYLYGF 240
                        DMLQTGLIVGGWDKY GGRIYGIPLGGTI+EQPFAIGGSGSSYLYGF
Sbjct: 181 -------------DMLQTGLIVGGWDKYNGGRIYGIPLGGTIIEQPFAIGGSGSSYLYGF 235

Query: 241 FDQAWREGMTREEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKFYPGDQLPLWH 300
           FDQAWREGMTREEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKFYPGDQLPLWH
Sbjct: 241 FDQAWREGMTREEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKFYPGDQLPLWH 235

Query: 301 EELEPKNSLLDVLNASSPEPMNI 324
           EELEPKNSLLDVLNASSPEPMN+
Sbjct: 301 EELEPKNSLLDVLNASSPEPMNV 235

[Close]

BLAST of Spg025704 vs. ExPASy TrEMBL
Match: A0A6J1K7T1 (Proteasome subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111491932 PE=3 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 9.6e-117
Identity = 231/323 (71.52%), Postives = 233/323 (72.14%), Query Frame = 0


Query: 1   MDLNMDLNAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRS 60
           MDLNMDLNAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRS
Sbjct: 1   MDLNMDLNAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRS 60

Query: 61  GSAADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNKDPCLFSEWVTAVGPV 120
           GSAADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNK               
Sbjct: 61  GSAADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNK--------------- 120

Query: 121 EDPQDPFSFLGAHISLFKIIACYIWLPENHNDIDPQDPSLFFKWVTAAGPVEDPQQPPTQ 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 SPTPKNAIGLNGQDMLQTGLIVGGWDKYEGGRIYGIPLGGTIVEQPFAIGGSGSSYLYGF 240
                        DMLQTGLIVGGWDKY GGRIYGIPLGGTI+EQPFAIGGSGSSYLYGF
Sbjct: 181 -------------DMLQTGLIVGGWDKYNGGRIYGIPLGGTIIEQPFAIGGSGSSYLYGF 235

Query: 241 FDQAWREGMTREEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKFYPGDQLPLWH 300
           FDQAWREGMTREEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGV RKFYPGDQLPLWH
Sbjct: 241 FDQAWREGMTREEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVARKFYPGDQLPLWH 235

Query: 301 EELEPKNSLLDVLNASSPEPMNI 324
           EELEPKNSLLDVLNASSPEPMN+
Sbjct: 301 EELEPKNSLLDVLNASSPEPMNV 235

[Close]

BLAST of Spg025704 vs. ExPASy TrEMBL
Match: A0A6J1BVE0 (Proteasome subunit beta OS=Momordica charantia OX=3673 GN=LOC111005067 PE=3 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 2.1e-116
Identity = 230/323 (71.21%), Postives = 234/323 (72.45%), Query Frame = 0


Query: 1   MDLNMDLNAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRS 60
           MDLNMDLNAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRS
Sbjct: 1   MDLNMDLNAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRS 60

Query: 61  GSAADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNKDPCLFSEWVTAVGPV 120
           GSAADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLL+YNNK               
Sbjct: 61  GSAADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLSYNNK--------------- 120

Query: 121 EDPQDPFSFLGAHISLFKIIACYIWLPENHNDIDPQDPSLFFKWVTAAGPVEDPQQPPTQ 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 SPTPKNAIGLNGQDMLQTGLIVGGWDKYEGGRIYGIPLGGTIVEQPFAIGGSGSSYLYGF 240
                        DMLQTGLIVGGWDKY+GGRIYGIPLGGTI+EQPFAIGGSGSSYLYGF
Sbjct: 181 -------------DMLQTGLIVGGWDKYDGGRIYGIPLGGTIIEQPFAIGGSGSSYLYGF 235

Query: 241 FDQAWREGMTREEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKFYPGDQLPLWH 300
           FDQAWREGM REEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKFYPGDQLPLWH
Sbjct: 241 FDQAWREGMIREEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKFYPGDQLPLWH 235

Query: 301 EELEPKNSLLDVLNASSPEPMNI 324
           EE+EPKNSLLDVLNASSPEPMNI
Sbjct: 301 EEIEPKNSLLDVLNASSPEPMNI 235

[Close]

BLAST of Spg025704 vs. ExPASy TrEMBL
Match: A0A6J1KTP7 (Proteasome subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111496312 PE=3 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 2.8e-116
Identity = 230/323 (71.21%), Postives = 234/323 (72.45%), Query Frame = 0


Query: 1   MDLNMDLNAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRS 60
           MDLNMDLNAPHSMGTTIIGVTY+GGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRS
Sbjct: 1   MDLNMDLNAPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRS 60

Query: 61  GSAADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNKDPCLFSEWVTAVGPV 120
           GSAADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNK               
Sbjct: 61  GSAADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNK--------------- 120

Query: 121 EDPQDPFSFLGAHISLFKIIACYIWLPENHNDIDPQDPSLFFKWVTAAGPVEDPQQPPTQ 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 SPTPKNAIGLNGQDMLQTGLIVGGWDKYEGGRIYGIPLGGTIVEQPFAIGGSGSSYLYGF 240
                        DMLQTGLIVGGWDKYEGGRIYGIPLGGTI+EQPFAIGGSGSSYLYGF
Sbjct: 181 -------------DMLQTGLIVGGWDKYEGGRIYGIPLGGTIIEQPFAIGGSGSSYLYGF 235

Query: 241 FDQAWREGMTREEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKFYPGDQLPLWH 300
           FDQAW+EGMTR+EAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKFYPGDQLPLWH
Sbjct: 241 FDQAWKEGMTRDEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKFYPGDQLPLWH 235

Query: 301 EELEPKNSLLDVLNASSPEPMNI 324
           EELEPKNSLLD LNASSPEPMNI
Sbjct: 301 EELEPKNSLLDALNASSPEPMNI 235

[Close]

BLAST of Spg025704 vs. ExPASy TrEMBL
Match: A0A6J1GHL6 (Proteasome subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111454253 PE=3 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 2.8e-116
Identity = 230/323 (71.21%), Postives = 234/323 (72.45%), Query Frame = 0


Query: 1   MDLNMDLNAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRS 60
           MDLNMDLNAPHSMGTTIIGVTY+GGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRS
Sbjct: 1   MDLNMDLNAPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRS 60

Query: 61  GSAADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNKDPCLFSEWVTAVGPV 120
           GSAADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNK               
Sbjct: 61  GSAADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNK--------------- 120

Query: 121 EDPQDPFSFLGAHISLFKIIACYIWLPENHNDIDPQDPSLFFKWVTAAGPVEDPQQPPTQ 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 SPTPKNAIGLNGQDMLQTGLIVGGWDKYEGGRIYGIPLGGTIVEQPFAIGGSGSSYLYGF 240
                        DMLQTGLIVGGWDKYEGGRIYGIPLGGTI+EQPFAIGGSGSSYLYGF
Sbjct: 181 -------------DMLQTGLIVGGWDKYEGGRIYGIPLGGTIIEQPFAIGGSGSSYLYGF 235

Query: 241 FDQAWREGMTREEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKFYPGDQLPLWH 300
           FDQAW+EGMTR+EAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKFYPGDQLPLWH
Sbjct: 241 FDQAWKEGMTRDEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKFYPGDQLPLWH 235

Query: 301 EELEPKNSLLDVLNASSPEPMNI 324
           EELEPKNSLLD LNASSPEPMNI
Sbjct: 301 EELEPKNSLLDALNASSPEPMNI 235

[Close]

BLAST of Spg025704 vs. TAIR 10
Match: AT4G31300.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 387.9 bits (995), Expect = 8.1e-108
Identity = 207/321 (64.49%), Postives = 226/321 (70.40%), Query Frame = 0


Query: 1   MDLNMDLNAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRS 60
           MDLN+D  APHSMGTTIIGVTY+GGVVLGADSRTSTG+YVANRASDKIT+LTDNVYVCRS
Sbjct: 1   MDLNLD--APHSMGTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRS 60

Query: 61  GSAADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNKDPCLFSEWVTAVGPV 120
           GSAADSQ+VSDYVR+FLHQHTIQ GQPATVKV+ANL+R+LAYNNK               
Sbjct: 61  GSAADSQVVSDYVRYFLHQHTIQHGQPATVKVSANLIRMLAYNNK--------------- 120

Query: 121 EDPQDPFSFLGAHISLFKIIACYIWLPENHNDIDPQDPSLFFKWVTAAGPVEDPQQPPTQ 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 SPTPKNAIGLNGQDMLQTGLIVGGWDKYEGGRIYGIPLGGTIVEQPFAIGGSGSSYLYGF 240
                       Q+MLQTGLIVGGWDKYEGG+IYGIPLGGT+VEQPFAIGGSGSSYLYGF
Sbjct: 181 ------------QNMLQTGLIVGGWDKYEGGKIYGIPLGGTVVEQPFAIGGSGSSYLYGF 232

Query: 241 FDQAWREGMTREEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKFYPGDQLPLWH 300
           FDQAW++ MT+EEAEQLVVKAVSLAIARDGASGGVVRTV INSEGVTR FYPGD+L LWH
Sbjct: 241 FDQAWKDNMTKEEAEQLVVKAVSLAIARDGASGGVVRTVIINSEGVTRNFYPGDKLQLWH 232

Query: 301 EELEPKNSLLDVLNASSPEPM 322
           EELEP+NSLLD+LNA+ PEPM
Sbjct: 301 EELEPQNSLLDILNAAGPEPM 232

[Close]

BLAST of Spg025704 vs. TAIR 10
Match: AT4G31300.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 385.6 bits (989), Expect = 4.0e-107
Identity = 206/321 (64.17%), Postives = 225/321 (70.09%), Query Frame = 0


Query: 1   MDLNMDLNAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRS 60
           MDLN+D  APHSMGTTIIGVTY+GGVVLGADSRTSTG+YVANRASDKIT+LTDNVYVCRS
Sbjct: 1   MDLNLD--APHSMGTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRS 60

Query: 61  GSAADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNKDPCLFSEWVTAVGPV 120
           GSAADSQ+VSDYVR+FLHQHTIQ GQPATVKV+ANL+R+LAYNNK               
Sbjct: 61  GSAADSQVVSDYVRYFLHQHTIQHGQPATVKVSANLIRMLAYNNK--------------- 120

Query: 121 EDPQDPFSFLGAHISLFKIIACYIWLPENHNDIDPQDPSLFFKWVTAAGPVEDPQQPPTQ 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 SPTPKNAIGLNGQDMLQTGLIVGGWDKYEGGRIYGIPLGGTIVEQPFAIGGSGSSYLYGF 240
                        +MLQTGLIVGGWDKYEGG+IYGIPLGGT+VEQPFAIGGSGSSYLYGF
Sbjct: 181 -------------NMLQTGLIVGGWDKYEGGKIYGIPLGGTVVEQPFAIGGSGSSYLYGF 231

Query: 241 FDQAWREGMTREEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKFYPGDQLPLWH 300
           FDQAW++ MT+EEAEQLVVKAVSLAIARDGASGGVVRTV INSEGVTR FYPGD+L LWH
Sbjct: 241 FDQAWKDNMTKEEAEQLVVKAVSLAIARDGASGGVVRTVIINSEGVTRNFYPGDKLQLWH 231

Query: 301 EELEPKNSLLDVLNASSPEPM 322
           EELEP+NSLLD+LNA+ PEPM
Sbjct: 301 EELEPQNSLLDILNAAGPEPM 231

[Close]

BLAST of Spg025704 vs. TAIR 10
Match: AT4G31300.3 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 385.6 bits (989), Expect = 4.0e-107
Identity = 206/321 (64.17%), Postives = 225/321 (70.09%), Query Frame = 0


Query: 1   MDLNMDLNAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRS 60
           MDLN+D  APHSMGTTIIGVTY+GGVVLGADSRTSTG+YVANRASDKIT+LTDNVYVCRS
Sbjct: 1   MDLNLD--APHSMGTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRS 60

Query: 61  GSAADSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNKDPCLFSEWVTAVGPV 120
           GSAADSQ+VSDYVR+FLHQHTIQ GQPATVKV+ANL+R+LAYNNK               
Sbjct: 61  GSAADSQVVSDYVRYFLHQHTIQHGQPATVKVSANLIRMLAYNNK--------------- 120

Query: 121 EDPQDPFSFLGAHISLFKIIACYIWLPENHNDIDPQDPSLFFKWVTAAGPVEDPQQPPTQ 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 SPTPKNAIGLNGQDMLQTGLIVGGWDKYEGGRIYGIPLGGTIVEQPFAIGGSGSSYLYGF 240
                        +MLQTGLIVGGWDKYEGG+IYGIPLGGT+VEQPFAIGGSGSSYLYGF
Sbjct: 181 -------------NMLQTGLIVGGWDKYEGGKIYGIPLGGTVVEQPFAIGGSGSSYLYGF 231

Query: 241 FDQAWREGMTREEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKFYPGDQLPLWH 300
           FDQAW++ MT+EEAEQLVVKAVSLAIARDGASGGVVRTV INSEGVTR FYPGD+L LWH
Sbjct: 241 FDQAWKDNMTKEEAEQLVVKAVSLAIARDGASGGVVRTVIINSEGVTRNFYPGDKLQLWH 231

Query: 301 EELEPKNSLLDVLNASSPEPM 322
           EELEP+NSLLD+LNA+ PEPM
Sbjct: 301 EELEPQNSLLDILNAAGPEPM 231

[Close]

BLAST of Spg025704 vs. TAIR 10
Match: AT5G40580.1 (20S proteasome beta subunit PBB2 )

HSP 1 Score: 74.3 bits (181), Expect = 2.0e-13
Identity = 70/272 (25.74%), Postives = 107/272 (39.34%), Query Frame = 0


Query: 7   LNAPHSM--GTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRSGSAA 66
           L AP  +  GTTI+G+ +  GV+LGAD+R + G  VA++  +KI  +  N+Y C +G+AA
Sbjct: 30  LKAPSFLKTGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAA 89

Query: 67  DSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNKDPCLFSEWVTAVGPVEDPQ 126
           D++ V+D V   L  H  Q G+ + V  A  L++                         +
Sbjct: 90  DTEAVTDMVSSQLRLHRYQTGRDSRVVTALTLLK-------------------------K 149

Query: 127 DPFSFLGAHISLFKIIACYIWLPENHNDIDPQDPSLFFKWVTAAGPVEDPQQPPTQSPTP 186
             FS+ G H+S                                                 
Sbjct: 150 HLFSYQG-HVS------------------------------------------------- 209

Query: 187 KNAIGLNGQDMLQTGLIVGGWDKYEGGRIYGIPLGGTIVEQPFAIGGSGSSYLYGFFDQA 246
                          L++GG D   G  ++ I   G+    PFA  GSGS      F+  
Sbjct: 210 -------------AALVLGGVD-ITGPHLHTIYPHGSTDTLPFATMGSGSLAAMSVFEAK 212

Query: 247 WREGMTREEAEQLVVKAVSLAIARDGASGGVV 277
           ++EG+TR+E  +LV +A+   I  D  SG  V
Sbjct: 270 YKEGLTRDEGIKLVAEAICSGIFNDLGSGSNV 212

[Close]

BLAST of Spg025704 vs. TAIR 10
Match: AT5G40580.2 (20S proteasome beta subunit PBB2 )

HSP 1 Score: 74.3 bits (181), Expect = 2.0e-13
Identity = 70/272 (25.74%), Postives = 107/272 (39.34%), Query Frame = 0


Query: 7   LNAPHSM--GTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRSGSAA 66
           L AP  +  GTTI+G+ +  GV+LGAD+R + G  VA++  +KI  +  N+Y C +G+AA
Sbjct: 30  LKAPSFLKTGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAA 89

Query: 67  DSQIVSDYVRHFLHQHTIQLGQPATVKVAANLVRLLAYNNKDPCLFSEWVTAVGPVEDPQ 126
           D++ V+D V   L  H  Q G+ + V  A  L++                         +
Sbjct: 90  DTEAVTDMVSSQLRLHRYQTGRDSRVVTALTLLK-------------------------K 149

Query: 127 DPFSFLGAHISLFKIIACYIWLPENHNDIDPQDPSLFFKWVTAAGPVEDPQQPPTQSPTP 186
             FS+ G H+S                                                 
Sbjct: 150 HLFSYQG-HVS------------------------------------------------- 209

Query: 187 KNAIGLNGQDMLQTGLIVGGWDKYEGGRIYGIPLGGTIVEQPFAIGGSGSSYLYGFFDQA 246
                          L++GG D   G  ++ I   G+    PFA  GSGS      F+  
Sbjct: 210 -------------AALVLGGVD-ITGPHLHTIYPHGSTDTLPFATMGSGSLAAMSVFEAK 212

Query: 247 WREGMTREEAEQLVVKAVSLAIARDGASGGVV 277
           ++EG+TR+E  +LV +A+   I  D  SG  V
Sbjct: 270 YKEGLTRDEGIKLVAEAICSGIFNDLGSGSNV 212

The following BLAST results are available for this feature:

BLAST of Spg025704 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038883996.1	3.1e-117	71.83	proteasome subunit beta type-6 [Benincasa hispida]	[more]
XP_022962546.1	5.2e-117	71.83	proteasome subunit beta type-6-like [Cucurbita moschata] >XP_023545809.1 proteas...	[more]
XP_022996805.1	2.0e-116	71.52	proteasome subunit beta type-6-like [Cucurbita maxima]	[more]
XP_022132123.1	4.4e-116	71.21	proteasome subunit beta type-6 [Momordica charantia]	[more]
XP_022951437.1	5.8e-116	71.21	proteasome subunit beta type-6 [Cucurbita moschata] >XP_023002488.1 proteasome s...	[more]

BLAST of Spg025704 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LD27	5.6e-106	64.17	Proteasome subunit beta type-6 OS=Arabidopsis thaliana OX=3702 GN=PBA1 PE=1 SV=2	[more]
P93395	2.4e-101	62.58	Proteasome subunit beta type-6 OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
Q55GJ6	8.3e-49	37.02	Proteasome subunit beta type-6 OS=Dictyostelium discoideum OX=44689 GN=psmB6 PE=...	[more]
Q3MHN0	4.5e-47	36.90	Proteasome subunit beta type-6 OS=Bos taurus OX=9913 GN=PSMB6 PE=1 SV=1	[more]
P28073	2.9e-46	36.21	Proteasome subunit beta type-6 OS=Rattus norvegicus OX=10116 GN=Psmb6 PE=1 SV=3	[more]

BLAST of Spg025704 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HDK0	2.5e-117	71.83	Proteasome subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111462944 PE=3 SV=1	[more]
A0A6J1K7T1	9.6e-117	71.52	Proteasome subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111491932 PE=3 SV=1	[more]
A0A6J1BVE0	2.1e-116	71.21	Proteasome subunit beta OS=Momordica charantia OX=3673 GN=LOC111005067 PE=3 SV=1	[more]
A0A6J1KTP7	2.8e-116	71.21	Proteasome subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111496312 PE=3 SV=1	[more]
A0A6J1GHL6	2.8e-116	71.21	Proteasome subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111454253 PE=3 SV=1	[more]

BLAST of Spg025704 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G31300.2	8.1e-108	64.49	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]
AT4G31300.1	4.0e-107	64.17	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]
AT4G31300.3	4.0e-107	64.17	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]
AT5G40580.1	2.0e-13	25.74	20S proteasome beta subunit PBB2	[more]
AT5G40580.2	2.0e-13	25.74	20S proteasome beta subunit PBB2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001353	Proteasome, subunit alpha/beta	PFAM	PF00227	Proteasome	coord: 13..112 e-value: 7.4E-23 score: 81.0 coord: 192..281 e-value: 6.2E-18 score: 65.0
IPR029055	Nucleophile aminohydrolases, N-terminal	GENE3D	3.60.20.10	Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1	coord: 15..302 e-value: 2.2E-59 score: 202.4
IPR029055	Nucleophile aminohydrolases, N-terminal	SUPERFAMILY	56235	N-terminal nucleophile aminohydrolases (Ntn hydrolases)	coord: 196..303
IPR029055	Nucleophile aminohydrolases, N-terminal	SUPERFAMILY	56235	N-terminal nucleophile aminohydrolases (Ntn hydrolases)	coord: 8..106
None	No IPR available	PIRSR	PIRSR600243-1	PIRSR600243-1	coord: 9..107 e-value: 2.3E-27 score: 93.3
None	No IPR available	PANTHER	PTHR11599	PROTEASOME SUBUNIT ALPHA/BETA	coord: 189..321
None	No IPR available	PANTHER	PTHR11599:SF151	PROTEASOME SUBUNIT BETA	coord: 7..107
None	No IPR available	PANTHER	PTHR11599:SF151	PROTEASOME SUBUNIT BETA	coord: 189..321
None	No IPR available	PANTHER	PTHR11599	PROTEASOME SUBUNIT ALPHA/BETA	coord: 7..107
None	No IPR available	CDD	cd03762	proteasome_beta_type_6	coord: 15..290 e-value: 7.80356E-109 score: 313.007
IPR016050	Proteasome beta-type subunit, conserved site	PROSITE	PS00854	PROTEASOME_BETA_1	coord: 18..65
IPR023333	Proteasome B-type subunit	PROSITE	PS51476	PROTEASOME_BETA_2	coord: 14..281 score: 34.657932

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg025704.1	Spg025704.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010498	proteasomal protein catabolic process
biological_process	GO:0051603	proteolysis involved in cellular protein catabolic process
cellular_component	GO:0019774	proteasome core complex, beta-subunit complex
cellular_component	GO:0005839	proteasome core complex
molecular_function	GO:0004298	threonine-type endopeptidase activity