Spg025663 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg025663
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	pectinesterase inhibitor 12-like
Location	scaffold13: 29890192 .. 29890710 (+)
RNA-Seq Expression	Spg025663
Synteny	Spg025663

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGCTATGTGCCAGAGCGGGCAGGGGCAGGGGCAGGAGCTGGAGGTGGAGCAGGATCGGGAGCGAGAGCTGCTGAGTCTGAATCTGAATCTGAATCTGAATTTGGGATTGGACATCTGCCGGAAGGCGGACTTCCCGGCCCTGTGTCGGTCTGTGGTGAAGGGTGCGAAGGTCCCATCTGAGGCGACGGAGGCTACGATCAGGAAATTGATGTTCGAGACGAAGATGGCGATGGCGACGGCCCAGCGGCGCAGGTCGCACTCGCAGGCCATGGATGTGTGCTTGGAGAATTACGACGACGCGTACAGCAACCTGGAGACTTGCCTTTCGAACCTGGATAGCCACGACAAGCCGAGCCTGAACATCAACCTGTCTGCGGCATTGACCGACTATGTGACCTGCGACGATGCAGTGGCCGAAGAAGGCGAGGCTTCCCCCGTTACCCGAACAAACGAAATGCTGCGCGAAATGGCCAGCAATGGTTTATTTTTGTCTAGTTTGATTCGCTTTCACTGA

mRNA sequence

ATGGCGGCTATGTGCCAGAGCGGGCAGGGGCAGGGGCAGGAGCTGGAGGTGGAGCAGGATCGGGAGCGAGAGCTGCTGAGTCTGAATCTGAATCTGAATCTGAATTTGGGATTGGACATCTGCCGGAAGGCGGACTTCCCGGCCCTGTGTCGGTCTGTGGTGAAGGGTGCGAAGGTCCCATCTGAGGCGACGGAGGCTACGATCAGGAAATTGATGTTCGAGACGAAGATGGCGATGGCGACGGCCCAGCGGCGCAGGTCGCACTCGCAGGCCATGGATGTGTGCTTGGAGAATTACGACGACGCGTACAGCAACCTGGAGACTTGCCTTTCGAACCTGGATAGCCACGACAAGCCGAGCCTGAACATCAACCTGTCTGCGGCATTGACCGACTATGTGACCTGCGACGATGCAGTGGCCGAAGAAGGCGAGGCTTCCCCCGTTACCCGAACAAACGAAATGCTGCGCGAAATGGCCAGCAATGGTTTATTTTTGTCTAGTTTGATTCGCTTTCACTGA

Coding sequence (CDS)

ATGGCGGCTATGTGCCAGAGCGGGCAGGGGCAGGGGCAGGAGCTGGAGGTGGAGCAGGATCGGGAGCGAGAGCTGCTGAGTCTGAATCTGAATCTGAATCTGAATTTGGGATTGGACATCTGCCGGAAGGCGGACTTCCCGGCCCTGTGTCGGTCTGTGGTGAAGGGTGCGAAGGTCCCATCTGAGGCGACGGAGGCTACGATCAGGAAATTGATGTTCGAGACGAAGATGGCGATGGCGACGGCCCAGCGGCGCAGGTCGCACTCGCAGGCCATGGATGTGTGCTTGGAGAATTACGACGACGCGTACAGCAACCTGGAGACTTGCCTTTCGAACCTGGATAGCCACGACAAGCCGAGCCTGAACATCAACCTGTCTGCGGCATTGACCGACTATGTGACCTGCGACGATGCAGTGGCCGAAGAAGGCGAGGCTTCCCCCGTTACCCGAACAAACGAAATGCTGCGCGAAATGGCCAGCAATGGTTTATTTTTGTCTAGTTTGATTCGCTTTCACTGA

Protein sequence

MAAMCQSGQGQGQELEVEQDRERELLSLNLNLNLNLGLDICRKADFPALCRSVVKGAKVPSEATEATIRKLMFETKMAMATAQRRRSHSQAMDVCLENYDDAYSNLETCLSNLDSHDKPSLNINLSAALTDYVTCDDAVAEEGEASPVTRTNEMLREMASNGLFLSSLIRFH

Homology

[Close]

BLAST of Spg025663 vs. NCBI nr
Match: XP_022131971.1 (pectinesterase inhibitor 12-like [Momordica charantia])

HSP 1 Score: 199.5 bits (506), Expect = 2.3e-47
Identity = 109/169 (64.50%), Postives = 126/169 (74.56%), Query Frame = 0


Query: 1   MAAMCQSGQGQGQELEVEQDRERELLSLNLNLNLNLGLDICRKADFPALCRSVVKGAKVP 60
           MA M   G  QGQ     Q+REREL  L L++NLNLG DICRKAD+PA+CRSVVKG   P
Sbjct: 14  MAGMWYGGHAQGQ----SQEREREL--LGLSINLNLGFDICRKADYPAMCRSVVKGLNNP 73

Query: 61  SEATEATIRKLMFETKMAMATAQRRRSHSQAMDVCLENYDDAYSNLETCLSNLDSHDKPS 120
             A EA +R+LMF+T  A + AQR +  S AM+VC E YDDAYSNLET LSNL SHDK S
Sbjct: 74  RVAFEAAVRQLMFQTSRAKSVAQRNKDKSSAMEVCFEVYDDAYSNLETGLSNLKSHDKSS 133

Query: 121 LNINLSAALTDYVTCDDAVAEEGEASPVTRTNEMLREMASNGLFLSSLI 170
           LN++LSA LTDYVTCDDA+ EEG  SPVTRTN +L +MASN L+ SSLI
Sbjct: 134 LNVDLSAVLTDYVTCDDAINEEGITSPVTRTNAILSQMASNCLYFSSLI 176

[Close]

BLAST of Spg025663 vs. NCBI nr
Match: XP_008444356.1 (PREDICTED: uncharacterized protein LOC103487709 [Cucumis melo] >KAA0061069.1 putative pectinesterase/pectinesterase inhibitor 40 [Cucumis melo var. makuwa])

HSP 1 Score: 192.2 bits (487), Expect = 3.7e-45
Identity = 99/143 (69.23%), Postives = 117/143 (81.82%), Query Frame = 0


Query: 30  LNLNLNLGLDICRKADFPALCRSVVKGAKVPSEATEATIRKLMFETKMAMATAQRRRSHS 89
           L LNLNL LD+C+KAD+PALCRSVVKG   PS A E +I+KLM ETK AM+ A+R++  S
Sbjct: 35  LTLNLNLSLDLCKKADYPALCRSVVKGLTDPSTAMETSIKKLMVETKQAMSVAKRQK--S 94

Query: 90  QAMDVCLENYDDAYSNLETCLSNLDSHDKPSLNINLSAALTDYVTCDDAVAEEGEASPVT 149
            AMDVC+E YDDAYSNLET LS+L SHDK +LNINLSA+LTDYVTCDDA+AE G  SPVT
Sbjct: 95  SAMDVCIEVYDDAYSNLETSLSSLKSHDKGTLNINLSASLTDYVTCDDAIAERGSTSPVT 154

Query: 150 RTNEMLREMASNGLFLSSLIRFH 173
           R N++LREM +N L+LS LIR H
Sbjct: 155 RNNKLLREMTTNCLYLSGLIRLH 175

[Close]

BLAST of Spg025663 vs. NCBI nr
Match: XP_038886537.1 (uncharacterized protein LOC120076712 [Benincasa hispida])

HSP 1 Score: 191.8 bits (486), Expect = 4.8e-45
Identity = 100/138 (72.46%), Postives = 118/138 (85.51%), Query Frame = 0


Query: 32  LNLNLGLDICRKADFPALCRSVVKGAKVPSEATEATIRKLMFETKMAMATAQRRRSHSQA 91
           ++LNL LD+C++ADFPALCRSVVKG   PS A E+TI++LMF+TK AM  A+RR+  S A
Sbjct: 32  ISLNLSLDLCQRADFPALCRSVVKGIHDPSVAIESTIKQLMFQTKQAMNVARRRK--SSA 91

Query: 92  MDVCLENYDDAYSNLETCLSNLDSHDKPSLNINLSAALTDYVTCDDAVAEEGEASPVTRT 151
           MDVC+E YDDAYSNLETCLS+L SHDK +LNINLSAALTDYVTCDDAVAE G  SPVTRT
Sbjct: 92  MDVCIEVYDDAYSNLETCLSSLQSHDKGTLNINLSAALTDYVTCDDAVAEIGLISPVTRT 151

Query: 152 NEMLREMASNGLFLSSLI 170
           N+++R MASN L+LSSLI
Sbjct: 152 NDLMRRMASNCLYLSSLI 167

[Close]

BLAST of Spg025663 vs. NCBI nr
Match: XP_004143062.2 (pectinesterase inhibitor 12 [Cucumis sativus] >KGN62312.1 hypothetical protein Csa_018682 [Cucumis sativus])

HSP 1 Score: 188.3 bits (477), Expect = 5.4e-44
Identity = 96/143 (67.13%), Postives = 117/143 (81.82%), Query Frame = 0


Query: 30  LNLNLNLGLDICRKADFPALCRSVVKGAKVPSEATEATIRKLMFETKMAMATAQRRRSHS 89
           L LNLNL LD+C+KAD+PALCRSVVKG   PS A E +I++LM ETK AM+ A+R++  S
Sbjct: 38  LTLNLNLSLDLCKKADYPALCRSVVKGLVDPSTAMETSIKQLMVETKQAMSVAKRQK--S 97

Query: 90  QAMDVCLENYDDAYSNLETCLSNLDSHDKPSLNINLSAALTDYVTCDDAVAEEGEASPVT 149
            AMDVC+E YDDAYSNLETCLS+L SHDK +LNINLSAALTDYVTC DA+AE+G +SP+T
Sbjct: 98  SAMDVCIEVYDDAYSNLETCLSSLKSHDKGTLNINLSAALTDYVTCQDAIAEKGLSSPIT 157

Query: 150 RTNEMLREMASNGLFLSSLIRFH 173
           R N +L +M +N L+LS LIR H
Sbjct: 158 RNNNLLSQMTTNCLYLSGLIRLH 178

[Close]

BLAST of Spg025663 vs. NCBI nr
Match: XP_023536926.1 (uncharacterized protein LOC111798158 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 186.4 bits (472), Expect = 2.0e-43
Identity = 106/169 (62.72%), Postives = 125/169 (73.96%), Query Frame = 0


Query: 1   MAAMCQSGQGQGQELEVEQDRERELLSLNLNLNLNLGLDICRKADFPALCRSVVKGAKVP 60
           MA M + GQGQGQE      +ERELLSLNL+ N+    D+C KA++P LCRSVVKG   P
Sbjct: 17  MAGMWEGGQGQGQE------QERELLSLNLDPNMG---DLCGKAEYPVLCRSVVKGLSKP 76

Query: 61  SEATEATIRKLMFETKMAMATAQRRRSHSQAMDVCLENYDDAYSNLETCLSNLDSHDKPS 120
           S A E+ IR+LM +TKMA  TA+R R  S  M+VC E Y DA+ NL+TCLS L + DKP+
Sbjct: 77  SVALESAIRQLMLQTKMARTTAKRLRK-SPGMEVCSETYKDAFGNLKTCLSTLKTGDKPT 136

Query: 121 LNINLSAALTDYVTCDDAVAEEGEASPVTRTNEMLREMASNGLFLSSLI 170
           LNINLSAALT YVTCDDAVAE G  SPVTRTN  LR+MA+N L+LSSLI
Sbjct: 137 LNINLSAALTGYVTCDDAVAERGGTSPVTRTNLTLRQMATNCLYLSSLI 175

[Close]

BLAST of Spg025663 vs. ExPASy Swiss-Prot
Match: Q9FJR5 (Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 2.3e-05
Identity = 29/73 (39.73%), Postives = 41/73 (56.16%), Query Frame = 0


Query: 83  QRRRSHSQAMDVCLENYDDAYSNLETCLSNLDSHDKPSLNINLSAALTDYVTCDDAVAEE 142
           QR +   + + +CL  Y+DA  +L T L+N+ S D    NINLSAAL     C+D   E 
Sbjct: 88  QRYQDIVEDLKLCLGFYNDANDDLTTALANIKSRDYQGANINLSAALDVPGNCEDDFKEA 147

Query: 143 GEASPVTRTNEML 156
            + SP+T  N +L
Sbjct: 148 KKTSPITNENSIL 160

[Close]

BLAST of Spg025663 vs. ExPASy Swiss-Prot
Match: A9YUH4 (Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 3.0e-05
Identity = 28/76 (36.84%), Postives = 47/76 (61.84%), Query Frame = 0


Query: 95  CLENYDDAYSNLETCLSNLDSHDKPSLNINLSAALTDYVTCDDAVAE-EGEASPVTRTNE 154
           C+  Y DA S+++  +++  S D  S N+ +SAAL D VTC+D   E +G ASPVT+ N+
Sbjct: 93  CVGLYADAKSSVQEAIADFKSKDYASANVKMSAALDDSVTCEDGFKEKKGIASPVTKENK 152

Query: 155 MLREMASNGLFLSSLI 170
              ++ +  L ++ L+
Sbjct: 153 DYVQLTAISLAITKLL 168

[Close]

BLAST of Spg025663 vs. ExPASy Swiss-Prot
Match: Q8GT41 (Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 8.7e-05
Identity = 27/76 (35.53%), Postives = 46/76 (60.53%), Query Frame = 0


Query: 95  CLENYDDAYSNLETCLSNLDSHDKPSLNINLSAALTDYVTCDDAVAE-EGEASPVTRTNE 154
           C+  Y DA S+++  +++  S D  S N+ +SAAL D VTC+D   E +G  SPVT+ N+
Sbjct: 102 CVGLYADAKSSVQEAIADFKSKDYASANVKMSAALDDSVTCEDGFKEKKGIVSPVTKENK 161

Query: 155 MLREMASNGLFLSSLI 170
              ++ +  L ++ L+
Sbjct: 162 DYVQLTAISLAITKLL 177

[Close]

BLAST of Spg025663 vs. ExPASy TrEMBL
Match: A0A6J1BQY5 (pectinesterase inhibitor 12-like OS=Momordica charantia OX=3673 GN=LOC111004955 PE=4 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 1.1e-47
Identity = 109/169 (64.50%), Postives = 126/169 (74.56%), Query Frame = 0


Query: 1   MAAMCQSGQGQGQELEVEQDRERELLSLNLNLNLNLGLDICRKADFPALCRSVVKGAKVP 60
           MA M   G  QGQ     Q+REREL  L L++NLNLG DICRKAD+PA+CRSVVKG   P
Sbjct: 14  MAGMWYGGHAQGQ----SQEREREL--LGLSINLNLGFDICRKADYPAMCRSVVKGLNNP 73

Query: 61  SEATEATIRKLMFETKMAMATAQRRRSHSQAMDVCLENYDDAYSNLETCLSNLDSHDKPS 120
             A EA +R+LMF+T  A + AQR +  S AM+VC E YDDAYSNLET LSNL SHDK S
Sbjct: 74  RVAFEAAVRQLMFQTSRAKSVAQRNKDKSSAMEVCFEVYDDAYSNLETGLSNLKSHDKSS 133

Query: 121 LNINLSAALTDYVTCDDAVAEEGEASPVTRTNEMLREMASNGLFLSSLI 170
           LN++LSA LTDYVTCDDA+ EEG  SPVTRTN +L +MASN L+ SSLI
Sbjct: 134 LNVDLSAVLTDYVTCDDAINEEGITSPVTRTNAILSQMASNCLYFSSLI 176

[Close]

BLAST of Spg025663 vs. ExPASy TrEMBL
Match: A0A5A7V5S8 (Putative pectinesterase/pectinesterase inhibitor 40 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G002070 PE=4 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 1.8e-45
Identity = 99/143 (69.23%), Postives = 117/143 (81.82%), Query Frame = 0


Query: 30  LNLNLNLGLDICRKADFPALCRSVVKGAKVPSEATEATIRKLMFETKMAMATAQRRRSHS 89
           L LNLNL LD+C+KAD+PALCRSVVKG   PS A E +I+KLM ETK AM+ A+R++  S
Sbjct: 35  LTLNLNLSLDLCKKADYPALCRSVVKGLTDPSTAMETSIKKLMVETKQAMSVAKRQK--S 94

Query: 90  QAMDVCLENYDDAYSNLETCLSNLDSHDKPSLNINLSAALTDYVTCDDAVAEEGEASPVT 149
            AMDVC+E YDDAYSNLET LS+L SHDK +LNINLSA+LTDYVTCDDA+AE G  SPVT
Sbjct: 95  SAMDVCIEVYDDAYSNLETSLSSLKSHDKGTLNINLSASLTDYVTCDDAIAERGSTSPVT 154

Query: 150 RTNEMLREMASNGLFLSSLIRFH 173
           R N++LREM +N L+LS LIR H
Sbjct: 155 RNNKLLREMTTNCLYLSGLIRLH 175

[Close]

BLAST of Spg025663 vs. ExPASy TrEMBL
Match: A0A1S3BAX0 (uncharacterized protein LOC103487709 OS=Cucumis melo OX=3656 GN=LOC103487709 PE=4 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 1.8e-45
Identity = 99/143 (69.23%), Postives = 117/143 (81.82%), Query Frame = 0


Query: 30  LNLNLNLGLDICRKADFPALCRSVVKGAKVPSEATEATIRKLMFETKMAMATAQRRRSHS 89
           L LNLNL LD+C+KAD+PALCRSVVKG   PS A E +I+KLM ETK AM+ A+R++  S
Sbjct: 35  LTLNLNLSLDLCKKADYPALCRSVVKGLTDPSTAMETSIKKLMVETKQAMSVAKRQK--S 94

Query: 90  QAMDVCLENYDDAYSNLETCLSNLDSHDKPSLNINLSAALTDYVTCDDAVAEEGEASPVT 149
            AMDVC+E YDDAYSNLET LS+L SHDK +LNINLSA+LTDYVTCDDA+AE G  SPVT
Sbjct: 95  SAMDVCIEVYDDAYSNLETSLSSLKSHDKGTLNINLSASLTDYVTCDDAIAERGSTSPVT 154

Query: 150 RTNEMLREMASNGLFLSSLIRFH 173
           R N++LREM +N L+LS LIR H
Sbjct: 155 RNNKLLREMTTNCLYLSGLIRLH 175

[Close]

BLAST of Spg025663 vs. ExPASy TrEMBL
Match: A0A0A0LNC2 (Invertase/pectin methylesterase inhibitor family protein OS=Cucumis sativus OX=3659 GN=Csa_2G348980 PE=4 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 2.6e-44
Identity = 96/143 (67.13%), Postives = 117/143 (81.82%), Query Frame = 0


Query: 30  LNLNLNLGLDICRKADFPALCRSVVKGAKVPSEATEATIRKLMFETKMAMATAQRRRSHS 89
           L LNLNL LD+C+KAD+PALCRSVVKG   PS A E +I++LM ETK AM+ A+R++  S
Sbjct: 38  LTLNLNLSLDLCKKADYPALCRSVVKGLVDPSTAMETSIKQLMVETKQAMSVAKRQK--S 97

Query: 90  QAMDVCLENYDDAYSNLETCLSNLDSHDKPSLNINLSAALTDYVTCDDAVAEEGEASPVT 149
            AMDVC+E YDDAYSNLETCLS+L SHDK +LNINLSAALTDYVTC DA+AE+G +SP+T
Sbjct: 98  SAMDVCIEVYDDAYSNLETCLSSLKSHDKGTLNINLSAALTDYVTCQDAIAEKGLSSPIT 157

Query: 150 RTNEMLREMASNGLFLSSLIRFH 173
           R N +L +M +N L+LS LIR H
Sbjct: 158 RNNNLLSQMTTNCLYLSGLIRLH 178

[Close]

BLAST of Spg025663 vs. ExPASy TrEMBL
Match: A0A6J1GK21 (pectinesterase inhibitor 12-like OS=Cucurbita moschata OX=3662 GN=LOC111454582 PE=4 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 3.5e-41
Identity = 102/169 (60.36%), Postives = 120/169 (71.01%), Query Frame = 0


Query: 1   MAAMCQSGQGQGQELEVEQDRERELLSLNLNLNLNLGLDICRKADFPALCRSVVKGAKVP 60
           MA M + GQGQG          RELLSLNL+ N+    D+C KAD+P LCRSVVKG   P
Sbjct: 1   MAGMWEGGQGQG----------RELLSLNLDPNMG---DLCGKADYPVLCRSVVKGVSKP 60

Query: 61  SEATEATIRKLMFETKMAMATAQRRRSHSQAMDVCLENYDDAYSNLETCLSNLDSHDKPS 120
           S A E+ IR+LM +TKMA  TA+R R  S  ++VC E Y DA+ NL+TCLS L + DKP+
Sbjct: 61  SVALESAIRQLMLQTKMARTTAKRLRK-SPGVEVCSETYQDAFRNLKTCLSTLKTGDKPT 120

Query: 121 LNINLSAALTDYVTCDDAVAEEGEASPVTRTNEMLREMASNGLFLSSLI 170
           LNINLSAALT YVTCDDAV E G  SPVTRTN  LR+MA+N L+LSSLI
Sbjct: 121 LNINLSAALTGYVTCDDAVVERGGTSPVTRTNLTLRQMATNCLYLSSLI 155

[Close]

BLAST of Spg025663 vs. TAIR 10
Match: AT1G23350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 105.5 bits (262), Expect = 4.3e-23
Identity = 60/147 (40.82%), Postives = 90/147 (61.22%), Query Frame = 0


Query: 25  LLSLNLNLNLNLGL-DICRKADFPALCRSVVKGAKVPSEATEATIRKLMFETKMAMATAQ 84
           LL+   +  L++ L D C  +DFPALCRS +KG    + AT+  I++LM  T+ A   A+
Sbjct: 12  LLAATSSQALSISLADPCTVSDFPALCRSTIKGQTNVNAATDVAIKELMKRTRQAKKIAE 71

Query: 85  RRRSHSQAMDVCLENYDDAYSNLETCLSNLDSHDKPSLNINLSAALTDYVTCDDAVAEEG 144
           +       +  CL N++ A+ NLE  L+N+  +D  SLNINLSAALTDY TC D++ E  
Sbjct: 72  KELKRDGGVATCLSNFNSAFDNLEKALTNIKENDGFSLNINLSAALTDYDTCSDSMKETK 131

Query: 145 EASPVTRTNEMLREMASNGLFLSSLIR 171
           E + + ++  +L  MA N L LS+L++
Sbjct: 132 EVNVIYKSAGVLYRMADNCLALSTLVK 158

[Close]

BLAST of Spg025663 vs. TAIR 10
Match: AT1G70540.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 102.8 bits (255), Expect = 2.8e-22
Identity = 59/134 (44.03%), Postives = 79/134 (58.96%), Query Frame = 0


Query: 37  GLDICRKADFPALCRSVVKGAKVPSEATEATIRKLMFETKMAMATAQRRRSHSQAMDVCL 96
           G D C  +DF  LCRSVVKG K  + ATE +IR+LM  T  A   A+  R     +  C 
Sbjct: 33  GRDPCSVSDFKVLCRSVVKGQKNVNAATEVSIRELMKRTIKAKEAAKISRKSGGGLKTCY 92

Query: 97  ENYDDAYSNLETCLSNLDSHDKPSLNINLSAALTDYVTCDDAVAEEGEASPVTRTNEMLR 156
            NYD A  NL+  L N+  +D  SLNINLSA+LTD+ TC+DA+     ++   ++   L 
Sbjct: 93  SNYDSALENLQKALKNIKQNDGFSLNINLSASLTDFDTCNDAMGGGTASNVFAKSTSTLH 152

Query: 157 EMASNGLFLSSLIR 171
           EMA N L LS+L++
Sbjct: 153 EMADNCLALSTLVK 166

[Close]

BLAST of Spg025663 vs. TAIR 10
Match: AT1G10770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 85.5 bits (210), Expect = 4.6e-17
Identity = 48/133 (36.09%), Postives = 77/133 (57.89%), Query Frame = 0


Query: 38  LDICRKADFPALCRSVVKGAKVPSEATEATIRKLMFETKMAMATAQRRRSHSQAMDVCLE 97
           ++IC    +P+LCR +VK    P +AT  TI+ L  +TK+A+A   R ++ +QA+  C E
Sbjct: 35  INICSHTAYPSLCRPLVKRVTSPRKATHRTIQALEAKTKLALAETARFKNGNQAVSTCYE 94

Query: 98  NYDDAYSNLETCLSNLDSHDKPSLNINLSAALTDYVTCDDAVAEEGEASPVTRTNEMLRE 157
              DA  NL +   ++   D P++N  L+AA++DY  C D   E  + + +      LR+
Sbjct: 95  TLGDAVYNLASARKSIRKRDVPAMNTYLTAAVSDYGACVDGFIETQQVNAIQNAVVDLRK 154

Query: 158 MASNGLFLSSLIR 171
           ++SN L LS+LIR
Sbjct: 155 ISSNCLALSTLIR 167

[Close]

BLAST of Spg025663 vs. TAIR 10
Match: AT3G36659.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 77.4 bits (189), Expect = 1.2e-14
Identity = 54/137 (39.42%), Postives = 72/137 (52.55%), Query Frame = 0


Query: 40  ICRKADFPALCRSVVKGAKVP------SEATEATIRKLMFETKMAMATAQRRRSHSQAMD 99
           IC K D P LC S V     P      S      I+  +  TK AMA  ++      A D
Sbjct: 133 ICGKTDNPPLCESSVSPLLTPQLKPNTSSVLILAIQASITATKAAMAIVEK----VDASD 192

Query: 100 VCLENYDDAYSNLETCLSNLDSHDKPSLNINLSAALTDYVTCDDAVAEEGEASPVTRTNE 159
            C E YDDA  NLE  ++ + S D  ++N NLSAA+TDY TC+D   E GE +P+    +
Sbjct: 193 -CQELYDDAVVNLEDAVNAVKSSDIATVNTNLSAAMTDYSTCNDGFEESGEPNPLAYVAD 252

Query: 160 MLREMASNGLFLSSLIR 171
            L +M SN L +S+LI+
Sbjct: 253 KLTKMVSNCLAISTLIK 264

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BLAST of Spg025663 vs. TAIR 10
Match: AT1G02550.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 51.2 bits (121), Expect = 9.5e-07
Identity = 39/146 (26.71%), Postives = 65/146 (44.52%), Query Frame = 0


Query: 40  ICRKADFPALC--------RSVVKGAKVPSEATEATIRKLMFETKMAMATAQR------- 99
           +C   D+ A C        R   K    P +     +  L  +    +  A+R       
Sbjct: 95  VCSNTDYAAECIVSILPLLRDFRKFEPKPIDVLRMEMSALYEKANATLDLAKRLIVDKST 154

Query: 100 RRSHSQAMDVCLENYDDAYSNLETCLSNLDSHDKPSLNINLSAALTDYVTCDDAVAEEGE 159
            R  +  +D+C++NY+    +L+     +D  D   L   +SAA+ D VTC DA AE  E
Sbjct: 155 PRDVADVLDLCVDNYESLLDDLKDASVAVDDGDFERLESVVSAAIADVVTCSDAFAESSE 214

Query: 160 -ASPVTRTNEMLREMASNGLFLSSLI 170
             SP+   ++ L+++ SN L +S +I
Sbjct: 215 LESPMANVDDFLKKLCSNVLAISQMI 240

The following BLAST results are available for this feature:

BLAST of Spg025663 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022131971.1	2.3e-47	64.50	pectinesterase inhibitor 12-like [Momordica charantia]	[more]
XP_008444356.1	3.7e-45	69.23	PREDICTED: uncharacterized protein LOC103487709 [Cucumis melo] >KAA0061069.1 put...	[more]
XP_038886537.1	4.8e-45	72.46	uncharacterized protein LOC120076712 [Benincasa hispida]	[more]
XP_004143062.2	5.4e-44	67.13	pectinesterase inhibitor 12 [Cucumis sativus] >KGN62312.1 hypothetical protein C...	[more]
XP_023536926.1	2.0e-43	62.72	uncharacterized protein LOC111798158 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Spg025663 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FJR5	2.3e-05	39.73	Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1	[more]
A9YUH4	3.0e-05	36.84	Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1	[more]
Q8GT41	8.7e-05	35.53	Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1	[more]

BLAST of Spg025663 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BQY5	1.1e-47	64.50	pectinesterase inhibitor 12-like OS=Momordica charantia OX=3673 GN=LOC111004955 ...	[more]
A0A5A7V5S8	1.8e-45	69.23	Putative pectinesterase/pectinesterase inhibitor 40 OS=Cucumis melo var. makuwa ...	[more]
A0A1S3BAX0	1.8e-45	69.23	uncharacterized protein LOC103487709 OS=Cucumis melo OX=3656 GN=LOC103487709 PE=...	[more]
A0A0A0LNC2	2.6e-44	67.13	Invertase/pectin methylesterase inhibitor family protein OS=Cucumis sativus OX=3...	[more]
A0A6J1GK21	3.5e-41	60.36	pectinesterase inhibitor 12-like OS=Cucurbita moschata OX=3662 GN=LOC111454582 P...	[more]

BLAST of Spg025663 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G23350.1	4.3e-23	40.82	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G70540.1	2.8e-22	44.03	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G10770.1	4.6e-17	36.09	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT3G36659.1	1.2e-14	39.42	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G02550.1	9.5e-07	26.71	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 32..165 e-value: 2.4E-19 score: 80.3
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 39..163 e-value: 2.6E-17 score: 63.6
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 43..167 e-value: 2.8E-13 score: 47.5
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 38..170 e-value: 7.8E-21 score: 76.6
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 39..169
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 35..169
None	No IPR available	PANTHER	PTHR31707:SF270	PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR SUPERFAMILY PROTEIN	coord: 35..169
None	No IPR available	CDD	cd15800	PMEI-like_2	coord: 39..169 e-value: 1.58912E-44 score: 142.114

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg025663.1	Spg025663.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity