Spg025633 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg025633
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionpolygalacturonase 1 beta-like protein 3
Locationscaffold13: 28953799 .. 28956697 (-)
RNA-Seq ExpressionSpg025633
SyntenySpg025633
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCTCGTAGGGAGATTTTGTAGAGAGGCGGGCCGGAAAGCGAGGAGGCTAGGCCTCACAGAAAGAAAACAGTCGAATATTAAGTCAGTGTATGAGTTAAGATAATCATAATCAAGCATTTAGAGTTTCGTATTAAGAATTTGGCATTTCATTTCATTTCATTTAAAACACAAAAAGGCAGGTATAAACGCGTATCGGATTTGGTTGCAATGCAATTCGGAAAAACCCACAGAGACACAACACAAGTCCAAAAATAAAGAAAGAAAAAGCCCATAAATATAAATTTTGGGTGTGTGAAAAAGGAAAACCACTATAAATTTCTGCATACAACCGAACGAACCCAACAACCGCACACCAACATTCTCACCCCGACCTCTTCTCTCTTCATCAAAACCGAAATGCGCAGATTACCCGCGCCGCTTGTGGTTCCCCTCTCTCTCTTTATCTTCTTGCTTTCGCCTCTCAATGTAAGTCAATCTCCATCTCCATTTCACTCTTTCTTTCCCTCTGCAACTCCTCCCTTGTTCTGTTGTGAATTCTGAATTGTAAATTGTAAATTGCAGGTTTCGGCGGCCGGCGACCAGGATAACCCCTTTACTCCGAAAGCTTTTCTGAATCGTTATTGGAACAAGGAGGTCCGGAATGATTTGCCAAAACCCAGTTTCCTCTTCTCCAAGGTCTCTCCCTTGACGGCAGTGCAGTCGGCCACCTTCGCCAAGCTCGCAGCCGAAAACGCACTCTCCTCCCGCCTCCCCGAGTTCTGCTCCGCCGCTCATTTGCTCTGCTTCCCCGATTTGTCCCCCTCTTTGGCCAAGCACGACAGGGACTCCAACTTTGCCGTCTATTCCAATCGGAACTTCACCACTTACGGCACCCAGAGATCTGGTGGAATCGACTCCTTCAAGAACTATTCCAATGACGACAATGTCATCGTGGATTCCTTCCGCCGCTACAGCCACGAGGCATTGGGCCACAAGGACCAATTTACGACCTATGCCACCGGCACCAACGTCCCCGACCAAAGTTTCAACACTTATGGTTCCAAAGCCGTCGGAGGCGACGGCAAGTTCACGACCTACGAGGAATCCGTCAATGTCCCCAATCTCCGATTTACCACCTACTCCACCGACGCCACGGGAAGATCTCACACATTCAAGAGCTACAGTGAGAACGCAAACGCCGGAGATCAGTCGTTTGCCAACTACGGAAAACGTGGTAATGGGGGTCCCAACGAGTTCGCTGGTTACGGGACCAACGCAAACGTAGTAGGCTCGGGGTTTTCGAGCTACGGGTCTGACGGAAATGGGGCCAACGACACATTCACCAATTACGGCAACGAGCAGAACAACCCACAGAATAACTTCAGGAGCTATGGCGAGAGTGGGAATGGAGCGATTGAAAGCTTCTCCAGTTACAGGGACCAGGCGAATGTGGGGGATGATTCATTCCAATCCTACTCCAAGAACTCGAACTCGGCGAAGGTCAATTTCGCAAATTACGGGAAGTCGTTCAACGAGGGCACGGACAAATTCAGTGGCTACGGTCAGAATGCGAATGGGCAAAGCGTGGGATTCAAGATCTATGGGGTGAATACGACGTTCAAAGATTACGCGAAACAGGGGATTAGTTTTGCGAAATACAGCAACGTGAGCAGTGGGGGTGGGAAAATGGCGGTGAGCGGGAGTTTGGCGAAGAGGTGGGTAGAGCCCGGGAAGTTCTTCCGGGAGTCGATGCTGAAGAAGGGGAAGGTGATGGCGATGCCGGACATCAGAGATAAAATGCCGAAGAGGTCGTTCTTGCCTCGATCTATTGTGTCGAAGTTGCCGTTTTCGAATTCGAAAGTGGGAGTAATGAAGGAGATATTCCACGCAGGGGAGAACTCGTCGATGGAGACGATGATGACGGAGACGCTGAGGGAGTGCGAGAGGGCTCCGAGCGCGGGTGAGACGAAGCGGTGCGTGGGGTCGGTGGAAGACATGATAGACTTCGCGACGTCGGTGCTGGGGCGGAACGTGGTGGTCCGGACGACGGAGAACACGAAGGGGTCGAAGGGGAACGTAATGATTGGGGCGGTGAAGGGGATAAACGGAGGGAAGGTGACGCAGTCGGTGTCGTGCCACCAGAGCCTGTTCCCTTACCTACTCTACTACTGCCACTCGGTGCCCAAGGTTCGGGTTTACGAAGCGGATTTGCTGGATCCGAGCTCTAAGGCCACCATCAACCACGGAGTTGCGATCTGTCACTTGGACACCTCGGCCTGGAGTCCCGCTCACGGGGCCTTCATAGCTCTGGGCTCCGCTCCCGGTCGGGTGGAGGTGTGCCACTGGATCTTCGAGAACGACATGACCTGGGCCGTGGCCGACTGATCGCCGGAGGCTCTGGCTGTGGTCTAAGAAAAGTTAGGCCGCCGCACCTCACCCATAACATGTTTTAAGGTTAATTTTAAGTGGATCCTCCTCCTTTTGCTTTTCAATTGAATTCGAAATAGAGAAGGAAAAAAAATGATTTTCTTTTTCTTTTCTTTTTTTTGGTTTTTCCGAAAAAAGGGTAAATGTGATTGTATTGTAAGTTATTTAAAATTGCTGGAAGAAATGGAAGGGAATTTGATGGGAGTTAGAAGGTAGTTGAATGGAGATGTGGATGAGTGTAACCAAAGTAAATAAACCAAAGAATGCTAAGTGTGAAAAGATGAGATGAGATGAGGTGGTCCTGTACCCATATCTATGTGCCTCCATTGCTTTGTTCTCTTGCGAGGGAAATGCCATGAGACAGCTCACACACTTGCCTCTTGTCACTCTCTTTTTAAACTTGTCTTTCTCCTTTTTCTTTTTCTTCTTCTTTTTTTTTTTTTTTAATCATTTATTATTATTATTATTATACCCATTCCTTTACTGTTTAAT

mRNA sequence

ATGCGCAGATTACCCGCGCCGCTTGTGGTTCCCCTCTCTCTCTTTATCTTCTTGCTTTCGCCTCTCAATGTTTCGGCGGCCGGCGACCAGGATAACCCCTTTACTCCGAAAGCTTTTCTGAATCGTTATTGGAACAAGGAGGTCCGGAATGATTTGCCAAAACCCAGTTTCCTCTTCTCCAAGGTCTCTCCCTTGACGGCAGTGCAGTCGGCCACCTTCGCCAAGCTCGCAGCCGAAAACGCACTCTCCTCCCGCCTCCCCGAGTTCTGCTCCGCCGCTCATTTGCTCTGCTTCCCCGATTTGTCCCCCTCTTTGGCCAAGCACGACAGGGACTCCAACTTTGCCGTCTATTCCAATCGGAACTTCACCACTTACGGCACCCAGAGATCTGGTGGAATCGACTCCTTCAAGAACTATTCCAATGACGACAATGTCATCGTGGATTCCTTCCGCCGCTACAGCCACGAGGCATTGGGCCACAAGGACCAATTTACGACCTATGCCACCGGCACCAACGTCCCCGACCAAAGTTTCAACACTTATGGTTCCAAAGCCGTCGGAGGCGACGGCAAGTTCACGACCTACGAGGAATCCGTCAATGTCCCCAATCTCCGATTTACCACCTACTCCACCGACGCCACGGGAAGATCTCACACATTCAAGAGCTACAGTGAGAACGCAAACGCCGGAGATCAGTCGTTTGCCAACTACGGAAAACGTGGTAATGGGGGTCCCAACGAGTTCGCTGGTTACGGGACCAACGCAAACGTAGTAGGCTCGGGGTTTTCGAGCTACGGGTCTGACGGAAATGGGGCCAACGACACATTCACCAATTACGGCAACGAGCAGAACAACCCACAGAATAACTTCAGGAGCTATGGCGAGAGTGGGAATGGAGCGATTGAAAGCTTCTCCAGTTACAGGGACCAGGCGAATGTGGGGGATGATTCATTCCAATCCTACTCCAAGAACTCGAACTCGGCGAAGGTCAATTTCGCAAATTACGGGAAGTCGTTCAACGAGGGCACGGACAAATTCAGTGGCTACGGTCAGAATGCGAATGGGCAAAGCGTGGGATTCAAGATCTATGGGGTGAATACGACGTTCAAAGATTACGCGAAACAGGGGATTAGTTTTGCGAAATACAGCAACGTGAGCAGTGGGGGTGGGAAAATGGCGGTGAGCGGGAGTTTGGCGAAGAGGTGGGTAGAGCCCGGGAAGTTCTTCCGGGAGTCGATGCTGAAGAAGGGGAAGGTGATGGCGATGCCGGACATCAGAGATAAAATGCCGAAGAGGTCGTTCTTGCCTCGATCTATTGTGTCGAAGTTGCCGTTTTCGAATTCGAAAGTGGGAGTAATGAAGGAGATATTCCACGCAGGGGAGAACTCGTCGATGGAGACGATGATGACGGAGACGCTGAGGGAGTGCGAGAGGGCTCCGAGCGCGGGTGAGACGAAGCGGTGCGTGGGGTCGGTGGAAGACATGATAGACTTCGCGACGTCGGTGCTGGGGCGGAACGTGGTGGTCCGGACGACGGAGAACACGAAGGGGTCGAAGGGGAACGTAATGATTGGGGCGGTGAAGGGGATAAACGGAGGGAAGGTGACGCAGTCGGTGTCGTGCCACCAGAGCCTGTTCCCTTACCTACTCTACTACTGCCACTCGGTGCCCAAGGTTCGGGTTTACGAAGCGGATTTGCTGGATCCGAGCTCTAAGGCCACCATCAACCACGGAGTTGCGATCTGTCACTTGGACACCTCGGCCTGGAGTCCCGCTCACGGGGCCTTCATAGCTCTGGGCTCCGCTCCCGGTCGGGTGGAGGTGTGCCACTGGATCTTCGAGAACGACATGACCTGGGCCGTGGCCGACTGA

Coding sequence (CDS)

ATGCGCAGATTACCCGCGCCGCTTGTGGTTCCCCTCTCTCTCTTTATCTTCTTGCTTTCGCCTCTCAATGTTTCGGCGGCCGGCGACCAGGATAACCCCTTTACTCCGAAAGCTTTTCTGAATCGTTATTGGAACAAGGAGGTCCGGAATGATTTGCCAAAACCCAGTTTCCTCTTCTCCAAGGTCTCTCCCTTGACGGCAGTGCAGTCGGCCACCTTCGCCAAGCTCGCAGCCGAAAACGCACTCTCCTCCCGCCTCCCCGAGTTCTGCTCCGCCGCTCATTTGCTCTGCTTCCCCGATTTGTCCCCCTCTTTGGCCAAGCACGACAGGGACTCCAACTTTGCCGTCTATTCCAATCGGAACTTCACCACTTACGGCACCCAGAGATCTGGTGGAATCGACTCCTTCAAGAACTATTCCAATGACGACAATGTCATCGTGGATTCCTTCCGCCGCTACAGCCACGAGGCATTGGGCCACAAGGACCAATTTACGACCTATGCCACCGGCACCAACGTCCCCGACCAAAGTTTCAACACTTATGGTTCCAAAGCCGTCGGAGGCGACGGCAAGTTCACGACCTACGAGGAATCCGTCAATGTCCCCAATCTCCGATTTACCACCTACTCCACCGACGCCACGGGAAGATCTCACACATTCAAGAGCTACAGTGAGAACGCAAACGCCGGAGATCAGTCGTTTGCCAACTACGGAAAACGTGGTAATGGGGGTCCCAACGAGTTCGCTGGTTACGGGACCAACGCAAACGTAGTAGGCTCGGGGTTTTCGAGCTACGGGTCTGACGGAAATGGGGCCAACGACACATTCACCAATTACGGCAACGAGCAGAACAACCCACAGAATAACTTCAGGAGCTATGGCGAGAGTGGGAATGGAGCGATTGAAAGCTTCTCCAGTTACAGGGACCAGGCGAATGTGGGGGATGATTCATTCCAATCCTACTCCAAGAACTCGAACTCGGCGAAGGTCAATTTCGCAAATTACGGGAAGTCGTTCAACGAGGGCACGGACAAATTCAGTGGCTACGGTCAGAATGCGAATGGGCAAAGCGTGGGATTCAAGATCTATGGGGTGAATACGACGTTCAAAGATTACGCGAAACAGGGGATTAGTTTTGCGAAATACAGCAACGTGAGCAGTGGGGGTGGGAAAATGGCGGTGAGCGGGAGTTTGGCGAAGAGGTGGGTAGAGCCCGGGAAGTTCTTCCGGGAGTCGATGCTGAAGAAGGGGAAGGTGATGGCGATGCCGGACATCAGAGATAAAATGCCGAAGAGGTCGTTCTTGCCTCGATCTATTGTGTCGAAGTTGCCGTTTTCGAATTCGAAAGTGGGAGTAATGAAGGAGATATTCCACGCAGGGGAGAACTCGTCGATGGAGACGATGATGACGGAGACGCTGAGGGAGTGCGAGAGGGCTCCGAGCGCGGGTGAGACGAAGCGGTGCGTGGGGTCGGTGGAAGACATGATAGACTTCGCGACGTCGGTGCTGGGGCGGAACGTGGTGGTCCGGACGACGGAGAACACGAAGGGGTCGAAGGGGAACGTAATGATTGGGGCGGTGAAGGGGATAAACGGAGGGAAGGTGACGCAGTCGGTGTCGTGCCACCAGAGCCTGTTCCCTTACCTACTCTACTACTGCCACTCGGTGCCCAAGGTTCGGGTTTACGAAGCGGATTTGCTGGATCCGAGCTCTAAGGCCACCATCAACCACGGAGTTGCGATCTGTCACTTGGACACCTCGGCCTGGAGTCCCGCTCACGGGGCCTTCATAGCTCTGGGCTCCGCTCCCGGTCGGGTGGAGGTGTGCCACTGGATCTTCGAGAACGACATGACCTGGGCCGTGGCCGACTGA

Protein sequence

MRRLPAPLVVPLSLFIFLLSPLNVSAAGDQDNPFTPKAFLNRYWNKEVRNDLPKPSFLFSKVSPLTAVQSATFAKLAAENALSSRLPEFCSAAHLLCFPDLSPSLAKHDRDSNFAVYSNRNFTTYGTQRSGGIDSFKNYSNDDNVIVDSFRRYSHEALGHKDQFTTYATGTNVPDQSFNTYGSKAVGGDGKFTTYEESVNVPNLRFTTYSTDATGRSHTFKSYSENANAGDQSFANYGKRGNGGPNEFAGYGTNANVVGSGFSSYGSDGNGANDTFTNYGNEQNNPQNNFRSYGESGNGAIESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNANGQSVGFKIYGVNTTFKDYAKQGISFAKYSNVSSGGGKMAVSGSLAKRWVEPGKFFRESMLKKGKVMAMPDIRDKMPKRSFLPRSIVSKLPFSNSKVGVMKEIFHAGENSSMETMMTETLRECERAPSAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNVMIGAVKGINGGKVTQSVSCHQSLFPYLLYYCHSVPKVRVYEADLLDPSSKATINHGVAICHLDTSAWSPAHGAFIALGSAPGRVEVCHWIFENDMTWAVAD
Homology
BLAST of Spg025633 vs. NCBI nr
Match: XP_038884819.1 (polygalacturonase 1 beta-like protein 3 [Benincasa hispida])

HSP 1 Score: 1145.6 bits (2962), Expect = 0.0e+00
Identity = 571/623 (91.65%), Postives = 598/623 (95.99%), Query Frame = 0

Query: 1   MRRLPAPLVVPLSLFIFLLSPLNVSAAGDQDNPFTPKAFLNRYWNKEVRNDLPKPSFLFS 60
           M RLP PLVVPL LFIF LS LNV AAGD+DNPF+PKAFLNRYWNKEVRND PKPSFLFS
Sbjct: 1   MHRLPTPLVVPLYLFIFSLSALNVPAAGDEDNPFSPKAFLNRYWNKEVRNDFPKPSFLFS 60

Query: 61  KVSPLTAVQSATFAKLAAENALSSRLPEFCSAAHLLCFPDLSPSLAKHDRDSNFAVYSNR 120
           K SPLTAVQSA FAKLAA+NALSSRLP+FCSAA+LLCFPDLSPSLAKHDRDSNFAVYSN+
Sbjct: 61  KASPLTAVQSAAFAKLAAQNALSSRLPDFCSAANLLCFPDLSPSLAKHDRDSNFAVYSNK 120

Query: 121 NFTTYGTQRSGGIDSFKNYSNDDNVIVDSFRRYSHEALGHKDQFTTYATGTNVPDQSFNT 180
           NFTTYGTQRSGGIDSFKNYSN +NVIVDSFRRYSHEALGH DQF+TYAT TNVPDQSFNT
Sbjct: 121 NFTTYGTQRSGGIDSFKNYSNGENVIVDSFRRYSHEALGHTDQFSTYATDTNVPDQSFNT 180

Query: 181 YGSKAVGGDGKFTTYEESVNVPNLRFTTYSTDATGRSHTFKSYSENANAGDQSFANYGKR 240
           Y SKA+GGDGKFTTYE+SVNVPNLRFTTYS+DATGRS +FKSYSENANAGDQSFANYGKR
Sbjct: 181 YASKAIGGDGKFTTYEQSVNVPNLRFTTYSSDATGRSQSFKSYSENANAGDQSFANYGKR 240

Query: 241 GNGGPNEFAGYGTNANVVGSGFSSYGSDGNGANDTFTNYGNEQNNPQNNFRSYGESGNGA 300
           GNGGPNEF GYGTNANVVGSGFSSYG+DGNGANDTFTNYGN QNNPQNNFR+YGE GNGA
Sbjct: 241 GNGGPNEFTGYGTNANVVGSGFSSYGTDGNGANDTFTNYGNNQNNPQNNFRNYGEGGNGA 300

Query: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNANGQSVGF 360
           IESFSSYRDQANVGDDSFQSYSKNSNSAKV FANYGKSFNEGTDKFSGYGQN NGQSVGF
Sbjct: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVKFANYGKSFNEGTDKFSGYGQNGNGQSVGF 360

Query: 361 KIYGVNTTFKDYAKQGISFAKYSNVSSGGGKMAVSGSLAKRWVEPGKFFRESMLKKGKVM 420
           K+YGVNTTFKDYAKQGISFAKYSNVSSGG K +V GSLAKRWVEPGKFFRESML+KG VM
Sbjct: 361 KMYGVNTTFKDYAKQGISFAKYSNVSSGGAKTSVRGSLAKRWVEPGKFFRESMLRKGTVM 420

Query: 421 AMPDIRDKMPKRSFLPRSIVSKLPFSNSKVGVMKEIFHAGENSSMETMMTETLRECERAP 480
           AMPDIRDKMPKRSFLPRSI+SKLPFS+SK+GVMK+IFHAG+NSSMETMMTET+RECERAP
Sbjct: 421 AMPDIRDKMPKRSFLPRSILSKLPFSSSKLGVMKQIFHAGDNSSMETMMTETVRECERAP 480

Query: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNVMIGAVKGINGGKVTQSVS 540
           SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGN+MIGAVKGINGGKVT+SVS
Sbjct: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKGINGGKVTESVS 540

Query: 541 CHQSLFPYLLYYCHSVPKVRVYEADLLDPSSKATINHGVAICHLDTSAWSPAHGAFIALG 600
           CHQSLFPYLLYYCHSVPKVRVYEADLLDP+SKATINHGVAICHLDTSAWSPAHGAFIAL 
Sbjct: 541 CHQSLFPYLLYYCHSVPKVRVYEADLLDPTSKATINHGVAICHLDTSAWSPAHGAFIALS 600

Query: 601 SAPGRVEVCHWIFENDMTWAVAD 624
           SAPG+VEVCHWIFENDMTW VAD
Sbjct: 601 SAPGQVEVCHWIFENDMTWTVAD 623

BLAST of Spg025633 vs. NCBI nr
Match: XP_023546090.1 (polygalacturonase 1 beta-like protein 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1134.8 bits (2934), Expect = 0.0e+00
Identity = 568/623 (91.17%), Postives = 596/623 (95.67%), Query Frame = 0

Query: 1   MRRLPAPLVVPLSLFIFLLSPLNVSAAGDQDNPFTPKAFLNRYWNKEVRNDLPKPSFLFS 60
           M  LPAPL+V L LFIFLLSPLNV AA D+DNPFTPKAFLNRYWNKEVRNDLPKP+FLFS
Sbjct: 1   MHTLPAPLLVLLYLFIFLLSPLNVPAAADEDNPFTPKAFLNRYWNKEVRNDLPKPTFLFS 60

Query: 61  KVSPLTAVQSATFAKLAAENALSSRLPEFCSAAHLLCFPDLSPSLAKHDRDSNFAVYSNR 120
           KVSPLTAVQSA FAKLAA+NALSSRLPEFCSAA+LLCFPDLSPSLAKHD  SNFAVYSN+
Sbjct: 61  KVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHDSTSNFAVYSNK 120

Query: 121 NFTTYGTQRSGGIDSFKNYSNDDNVIVDSFRRYSHEALGHKDQFTTYATGTNVPDQSFNT 180
           NFTTYGTQRSGGIDSFKNYSN DNV+VDSFRRYSHEALGH DQF+TY T TNVPDQSFNT
Sbjct: 121 NFTTYGTQRSGGIDSFKNYSNGDNVVVDSFRRYSHEALGHTDQFSTYGTDTNVPDQSFNT 180

Query: 181 YGSKAVGGDGKFTTYEESVNVPNLRFTTYSTDATGRSHTFKSYSENANAGDQSFANYGKR 240
           Y SKAVGG+G FTTYE+SVNVPNLRFTTYS+DATGRS +FKSYSENANAGDQSF NYGKR
Sbjct: 181 YASKAVGGNGGFTTYEQSVNVPNLRFTTYSSDATGRSQSFKSYSENANAGDQSFVNYGKR 240

Query: 241 GNGGPNEFAGYGTNANVVGSGFSSYGSDGNGANDTFTNYGNEQNNPQNNFRSYGESGNGA 300
           GNGGPNEF GYGTNANVVGSGFSSYG++GNGANDTFTNYGNEQNNPQNNFRSYG+ GNGA
Sbjct: 241 GNGGPNEFTGYGTNANVVGSGFSSYGANGNGANDTFTNYGNEQNNPQNNFRSYGDGGNGA 300

Query: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNANGQSVGF 360
           IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQN NGQSVGF
Sbjct: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNGNGQSVGF 360

Query: 361 KIYGVNTTFKDYAKQGISFAKYSNVSSGGGKMAVSGSLAKRWVEPGKFFRESMLKKGKVM 420
           K YGVNTTFKDYAKQGISFAKYSNVSSGG KM+VS SLAKRWVEPGKFFRESML+KG VM
Sbjct: 361 KTYGVNTTFKDYAKQGISFAKYSNVSSGGAKMSVSESLAKRWVEPGKFFRESMLRKGTVM 420

Query: 421 AMPDIRDKMPKRSFLPRSIVSKLPFSNSKVGVMKEIFHAGENSSMETMMTETLRECERAP 480
           AMPDIRDKMPKRSFLPRSI+SKLPFS+SK+ VMK+IFHAG+NSSMETMMTETLR+CER P
Sbjct: 421 AMPDIRDKMPKRSFLPRSILSKLPFSSSKLEVMKQIFHAGDNSSMETMMTETLRDCERTP 480

Query: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNVMIGAVKGINGGKVTQSVS 540
           SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGN+MIGAVKGI GGKVT+SVS
Sbjct: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKGIEGGKVTESVS 540

Query: 541 CHQSLFPYLLYYCHSVPKVRVYEADLLDPSSKATINHGVAICHLDTSAWSPAHGAFIALG 600
           CHQSLFPYLLYYCHSVPKVRVYEA+LLDP+SKATINHGVAICHLDTSAWSPAHGAFIALG
Sbjct: 541 CHQSLFPYLLYYCHSVPKVRVYEAELLDPTSKATINHGVAICHLDTSAWSPAHGAFIALG 600

Query: 601 SAPGRVEVCHWIFENDMTWAVAD 624
           SAPGRVEVCHWIFENDMTW+VAD
Sbjct: 601 SAPGRVEVCHWIFENDMTWSVAD 623

BLAST of Spg025633 vs. NCBI nr
Match: XP_022961767.1 (polygalacturonase 1 beta-like protein 3 [Cucurbita moschata])

HSP 1 Score: 1134.4 bits (2933), Expect = 0.0e+00
Identity = 567/623 (91.01%), Postives = 596/623 (95.67%), Query Frame = 0

Query: 1   MRRLPAPLVVPLSLFIFLLSPLNVSAAGDQDNPFTPKAFLNRYWNKEVRNDLPKPSFLFS 60
           M  LPAPL+V L LFIFLLSPLNV AA D+DNPFTPKAFLNRYWNKEVRNDLPKP+FLFS
Sbjct: 1   MHTLPAPLLVLLYLFIFLLSPLNVPAAADEDNPFTPKAFLNRYWNKEVRNDLPKPTFLFS 60

Query: 61  KVSPLTAVQSATFAKLAAENALSSRLPEFCSAAHLLCFPDLSPSLAKHDRDSNFAVYSNR 120
           KVSPLTAVQSA FAKLAA+NALSSRLPEFCSAA+LLCFPDLSPSLAKHD  SNFAVYSN+
Sbjct: 61  KVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHDSTSNFAVYSNK 120

Query: 121 NFTTYGTQRSGGIDSFKNYSNDDNVIVDSFRRYSHEALGHKDQFTTYATGTNVPDQSFNT 180
           NFTTYGTQRSGGIDSFKNYSN DNV+VDSFRRYSHEALGH DQF+TY T TNVPDQSFNT
Sbjct: 121 NFTTYGTQRSGGIDSFKNYSNGDNVVVDSFRRYSHEALGHTDQFSTYGTDTNVPDQSFNT 180

Query: 181 YGSKAVGGDGKFTTYEESVNVPNLRFTTYSTDATGRSHTFKSYSENANAGDQSFANYGKR 240
           Y SKAVGG+G FTTYE+SVNVPNLRFTTYS+DATGRS +FKSYSENANAGDQSF NYGKR
Sbjct: 181 YASKAVGGNGGFTTYEQSVNVPNLRFTTYSSDATGRSQSFKSYSENANAGDQSFVNYGKR 240

Query: 241 GNGGPNEFAGYGTNANVVGSGFSSYGSDGNGANDTFTNYGNEQNNPQNNFRSYGESGNGA 300
           GNGGPNEF GYGTNANV+GSGFSSYG++GNGANDTFTNYGNEQNNPQNNFRSYG+ GNGA
Sbjct: 241 GNGGPNEFTGYGTNANVIGSGFSSYGANGNGANDTFTNYGNEQNNPQNNFRSYGDGGNGA 300

Query: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNANGQSVGF 360
           IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQN NGQSVGF
Sbjct: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNGNGQSVGF 360

Query: 361 KIYGVNTTFKDYAKQGISFAKYSNVSSGGGKMAVSGSLAKRWVEPGKFFRESMLKKGKVM 420
           K YGVNTTFKDYAKQGISFAKYSNVSSGG KM+VS SLAKRWVEPGKFFRESML+KG VM
Sbjct: 361 KTYGVNTTFKDYAKQGISFAKYSNVSSGGAKMSVSESLAKRWVEPGKFFRESMLRKGTVM 420

Query: 421 AMPDIRDKMPKRSFLPRSIVSKLPFSNSKVGVMKEIFHAGENSSMETMMTETLRECERAP 480
           AMPDIRDKMPKRSFLPRSI+SKLPFS+SK+ VMK+IFHAG+NSSMETMMTETLR+CER P
Sbjct: 421 AMPDIRDKMPKRSFLPRSILSKLPFSSSKLEVMKQIFHAGDNSSMETMMTETLRDCERTP 480

Query: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNVMIGAVKGINGGKVTQSVS 540
           SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGN+MIGAVKGI GGKVT+SVS
Sbjct: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKGIEGGKVTESVS 540

Query: 541 CHQSLFPYLLYYCHSVPKVRVYEADLLDPSSKATINHGVAICHLDTSAWSPAHGAFIALG 600
           CHQSLFPYLLYYCHSVPKVRVYEA+LLDP+SKATINHGVAICHLDTSAWSPAHGAFIALG
Sbjct: 541 CHQSLFPYLLYYCHSVPKVRVYEAELLDPTSKATINHGVAICHLDTSAWSPAHGAFIALG 600

Query: 601 SAPGRVEVCHWIFENDMTWAVAD 624
           SAPGRVEVCHWIFENDMTW+VAD
Sbjct: 601 SAPGRVEVCHWIFENDMTWSVAD 623

BLAST of Spg025633 vs. NCBI nr
Match: XP_008444317.1 (PREDICTED: polygalacturonase 1 beta-like protein 3 [Cucumis melo] >KAA0061089.1 polygalacturonase 1 beta-like protein 3 [Cucumis melo var. makuwa])

HSP 1 Score: 1133.6 bits (2931), Expect = 0.0e+00
Identity = 564/623 (90.53%), Postives = 593/623 (95.18%), Query Frame = 0

Query: 1   MRRLPAPLVVPLSLFIFLLSPLNVSAAGDQDNPFTPKAFLNRYWNKEVRNDLPKPSFLFS 60
           M RLP   VVPL LFIF LS LNV A GD+DNPFTPKAFLNRYWNKEVRND+PKPSFLFS
Sbjct: 1   MHRLPTLHVVPLYLFIFSLSALNVPAVGDEDNPFTPKAFLNRYWNKEVRNDIPKPSFLFS 60

Query: 61  KVSPLTAVQSATFAKLAAENALSSRLPEFCSAAHLLCFPDLSPSLAKHDRDSNFAVYSNR 120
           K SPLTAVQSA FAKLAAENALSSRLP+FCSAA+LLCFPDLSPSLAKHDRDSNFAVYSN+
Sbjct: 61  KASPLTAVQSAAFAKLAAENALSSRLPDFCSAANLLCFPDLSPSLAKHDRDSNFAVYSNK 120

Query: 121 NFTTYGTQRSGGIDSFKNYSNDDNVIVDSFRRYSHEALGHKDQFTTYATGTNVPDQSFNT 180
           NFTTYGTQRSGGIDSFKNYSN DNV+VDSFRRYSHEALGH D+F+TY T TNVPDQSFNT
Sbjct: 121 NFTTYGTQRSGGIDSFKNYSNGDNVVVDSFRRYSHEALGHTDRFSTYGTETNVPDQSFNT 180

Query: 181 YGSKAVGGDGKFTTYEESVNVPNLRFTTYSTDATGRSHTFKSYSENANAGDQSFANYGKR 240
           Y SKA+GGDGKFTTYE+SVNVPNLRFTTYS+DATGRS  FKSYSENANAGDQSF NYGKR
Sbjct: 181 YASKAIGGDGKFTTYEQSVNVPNLRFTTYSSDATGRSQAFKSYSENANAGDQSFVNYGKR 240

Query: 241 GNGGPNEFAGYGTNANVVGSGFSSYGSDGNGANDTFTNYGNEQNNPQNNFRSYGESGNGA 300
           GNGGPNEF GYGT+ANVVGS FSSYG+DGNGANDTFTNYGN+QNNPQNNFRSYGE GNGA
Sbjct: 241 GNGGPNEFTGYGTSANVVGSRFSSYGTDGNGANDTFTNYGNDQNNPQNNFRSYGEGGNGA 300

Query: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNANGQSVGF 360
           IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFA+YGKSFNEGTDKFSGYGQN NGQSVGF
Sbjct: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFASYGKSFNEGTDKFSGYGQNGNGQSVGF 360

Query: 361 KIYGVNTTFKDYAKQGISFAKYSNVSSGGGKMAVSGSLAKRWVEPGKFFRESMLKKGKVM 420
           K+YGVNTTFKDYAKQGI+FAKYSNVSSGG K +VSGSLAKRWVEPGKFFRESML+KG VM
Sbjct: 361 KVYGVNTTFKDYAKQGINFAKYSNVSSGGAKTSVSGSLAKRWVEPGKFFRESMLRKGSVM 420

Query: 421 AMPDIRDKMPKRSFLPRSIVSKLPFSNSKVGVMKEIFHAGENSSMETMMTETLRECERAP 480
           AMPDIRDKMPKRSFLPRSI+SKLPFS+SK+GVMK+IFHAG+NSSMETMMTET+RECER P
Sbjct: 421 AMPDIRDKMPKRSFLPRSILSKLPFSSSKLGVMKQIFHAGDNSSMETMMTETVRECERVP 480

Query: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNVMIGAVKGINGGKVTQSVS 540
           SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGN+MIG VKG+NGGKVT+SVS
Sbjct: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGKVKGMNGGKVTESVS 540

Query: 541 CHQSLFPYLLYYCHSVPKVRVYEADLLDPSSKATINHGVAICHLDTSAWSPAHGAFIALG 600
           CHQSLFPYLLYYCHSVPKVRVYE DLLDP+SKATINHGVAICHLDTSAWSPAHGAFIALG
Sbjct: 541 CHQSLFPYLLYYCHSVPKVRVYEVDLLDPASKATINHGVAICHLDTSAWSPAHGAFIALG 600

Query: 601 SAPGRVEVCHWIFENDMTWAVAD 624
           SAPGRVEVCHWIFENDMTW VAD
Sbjct: 601 SAPGRVEVCHWIFENDMTWTVAD 623

BLAST of Spg025633 vs. NCBI nr
Match: KAG6598207.1 (Polygalacturonase 1 beta-like protein 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1130.9 bits (2924), Expect = 0.0e+00
Identity = 565/623 (90.69%), Postives = 595/623 (95.51%), Query Frame = 0

Query: 1   MRRLPAPLVVPLSLFIFLLSPLNVSAAGDQDNPFTPKAFLNRYWNKEVRNDLPKPSFLFS 60
           M  LPAPL+V L LFIFLLSPLNV AA D+DNPFTPKAFLNRYWNKEVRNDLPKP+FLFS
Sbjct: 1   MHTLPAPLLVLLYLFIFLLSPLNVPAAADEDNPFTPKAFLNRYWNKEVRNDLPKPTFLFS 60

Query: 61  KVSPLTAVQSATFAKLAAENALSSRLPEFCSAAHLLCFPDLSPSLAKHDRDSNFAVYSNR 120
           KVSPLTAVQSA FAKLAA+NALSSRLPEFCSAA+LLCFPDLSPSLAKH+  SNFAVYSN+
Sbjct: 61  KVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHESTSNFAVYSNK 120

Query: 121 NFTTYGTQRSGGIDSFKNYSNDDNVIVDSFRRYSHEALGHKDQFTTYATGTNVPDQSFNT 180
           NFTTYGTQRSGGIDSFKNYSN DNV+VDSFRRYSHEALGH DQF+ Y T TNVPDQSFNT
Sbjct: 121 NFTTYGTQRSGGIDSFKNYSNGDNVVVDSFRRYSHEALGHTDQFSAYGTDTNVPDQSFNT 180

Query: 181 YGSKAVGGDGKFTTYEESVNVPNLRFTTYSTDATGRSHTFKSYSENANAGDQSFANYGKR 240
           Y SKAVGG+G FTTYE+SVNVPNLRFTTYS+DATGRS +FKSYSENANAGDQSF NYGKR
Sbjct: 181 YASKAVGGNGGFTTYEQSVNVPNLRFTTYSSDATGRSQSFKSYSENANAGDQSFVNYGKR 240

Query: 241 GNGGPNEFAGYGTNANVVGSGFSSYGSDGNGANDTFTNYGNEQNNPQNNFRSYGESGNGA 300
           GNGGPNEF GYGTNANV+GSGFSSYG++GNGANDTFTNYGNEQNNPQNNFRSYG+ GNGA
Sbjct: 241 GNGGPNEFTGYGTNANVIGSGFSSYGANGNGANDTFTNYGNEQNNPQNNFRSYGDGGNGA 300

Query: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNANGQSVGF 360
           IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQN NGQSVGF
Sbjct: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNGNGQSVGF 360

Query: 361 KIYGVNTTFKDYAKQGISFAKYSNVSSGGGKMAVSGSLAKRWVEPGKFFRESMLKKGKVM 420
           K YGVNTTFKDYAKQGISFAKYSNVSSGG KM+VS SLAKRWVEPGKFFRESML+KG VM
Sbjct: 361 KTYGVNTTFKDYAKQGISFAKYSNVSSGGAKMSVSESLAKRWVEPGKFFRESMLRKGTVM 420

Query: 421 AMPDIRDKMPKRSFLPRSIVSKLPFSNSKVGVMKEIFHAGENSSMETMMTETLRECERAP 480
           AMPDIRDKMPKRSFLPRSI+SKLPFS+SK+ VMK+IFHAG+NSSMETMMTETLR+CER P
Sbjct: 421 AMPDIRDKMPKRSFLPRSILSKLPFSSSKLEVMKQIFHAGDNSSMETMMTETLRDCERTP 480

Query: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNVMIGAVKGINGGKVTQSVS 540
           SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGN+MIGAVKGI GGKVT+SVS
Sbjct: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKGIEGGKVTESVS 540

Query: 541 CHQSLFPYLLYYCHSVPKVRVYEADLLDPSSKATINHGVAICHLDTSAWSPAHGAFIALG 600
           CHQSLFPYLLYYCHSVPKVRVYEA+LLDP+SKATINHGVAICHLDTSAWSPAHGAFIALG
Sbjct: 541 CHQSLFPYLLYYCHSVPKVRVYEAELLDPTSKATINHGVAICHLDTSAWSPAHGAFIALG 600

Query: 601 SAPGRVEVCHWIFENDMTWAVAD 624
           SAPGRVEVCHWIFENDMTW+VAD
Sbjct: 601 SAPGRVEVCHWIFENDMTWSVAD 623

BLAST of Spg025633 vs. ExPASy Swiss-Prot
Match: P92990 (Polygalacturonase 1 beta-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=PGL3 PE=2 SV=2)

HSP 1 Score: 796.6 bits (2056), Expect = 2.0e-229
Identity = 388/595 (65.21%), Postives = 470/595 (78.99%), Query Frame = 0

Query: 30  QDNPFTPKAFLNRYWNKEVRNDLPKPSFLFSKVSPLTAVQSATFAKLAAENALSSRLPEF 89
           ++NPFTPKA L RYWNKE+R   P+  FL SK SPL AV SATF+KLAA N+L +R P+F
Sbjct: 33  EENPFTPKASLVRYWNKEIRGQSPRSEFLISKASPLNAVDSATFSKLAAANSLPTRFPDF 92

Query: 90  CSAAHLLCFPDLSPSLAKHDRDSNFAVYSNRNFTTYGTQRSGGIDSFKNYSNDDNVIVDS 149
           CSAA+L CFPDL  SL KHD D  F+VY  +NFT YG  R+GG DSFKNYS D NV+ DS
Sbjct: 93  CSAANLFCFPDLGASLEKHDDDVKFSVYDQKNFTNYGNARAGGADSFKNYSKDGNVVTDS 152

Query: 150 FRRYSHEALGHKDQFTTYATGTNVPDQSFNTYGSKAVGGDGKFTTYEESVNVPNLRFTTY 209
           FRRYS  A GH D+FT Y   +NV ++ FN+YG+   GG G FT Y+ +VN P  RFT Y
Sbjct: 153 FRRYSRNAAGHDDKFTVYGENSNVVEEGFNSYGTFGTGGAGDFTNYQNNVNNPTSRFTAY 212

Query: 210 STDATGRSHTFKSYSENANAGD-QSFANYGKRGNGGPNEFAGYGTNANVVGSGFSSYGSD 269
           S    GRS TFK+Y+  ANAG+ QSF +YGK GNG PNEF  YG ++NV+GSGFS+YG  
Sbjct: 213 SDGGNGRSQTFKTYTHEANAGNGQSFTSYGKNGNGVPNEFTSYGVSSNVIGSGFSNYGES 272

Query: 270 GNGANDTFTNYGNEQNNPQNNFRSYGESGNGAIESFSSYRDQANVGDDSFQSYSKNSNSA 329
           GN ANDTFT+YG++ N PQNNF +YG SGN A+++F++YRD+ANVGDDSF SY+K+SNS 
Sbjct: 273 GNAANDTFTSYGSDGNVPQNNFNNYGASGNAAVDTFANYRDKANVGDDSFSSYAKDSNSE 332

Query: 330 KVNFANYGKSFNEGTDKFSGYGQNANGQSVGFKIYGVNTTFKDYAKQGISFAKYSNVSSG 389
           KVNF NYG+SFN G++ F+GYG+ A G  + FK Y  N+TFKDYAK+G++FAKY NVS+ 
Sbjct: 333 KVNFVNYGQSFNPGSETFTGYGKGAEGSKLSFKTYTPNSTFKDYAKKGVAFAKY-NVSTT 392

Query: 390 GGKMAVSGSLAKRWVEPGKFFRESMLKKGKVMAMPDIRDKMPKRSFLPRSIVSKLPFSNS 449
                  G    +W+EPGKFFRES LK+G V+ MPDI+DKMPKRSFLPRSI++KLPFS S
Sbjct: 393 TANTVGDGKTVNKWIEPGKFFRESSLKEGTVIPMPDIKDKMPKRSFLPRSIITKLPFSTS 452

Query: 450 KVGVMKEIFHAGENSSMETMMTETLRECERAPSAGETKRCVGSVEDMIDFATSVLGRNVV 509
           K+G +K IFHA ENS+M  ++T+ + ECER PS GETKRCVGS EDMIDFATSVLGR+VV
Sbjct: 453 KLGEIKRIFHAVENSTMGGIITDAVTECERPPSVGETKRCVGSAEDMIDFATSVLGRSVV 512

Query: 510 VRTTENTKGSKGNVMIGAVKGINGGKVTQSVSCHQSLFPYLLYYCHSVPKVRVYEADLLD 569
           +RTTEN  GSK  V+IG V GINGGK+T++VSCHQSL+PYLLYYCHSVPKVRVYEADLL+
Sbjct: 513 LRTTENVAGSKEKVVIGKVNGINGGKLTKAVSCHQSLYPYLLYYCHSVPKVRVYEADLLE 572

Query: 570 PSSKATINHGVAICHLDTSAWSPAHGAFIALGSAPGRVEVCHWIFENDMTWAVAD 624
            +SK  INHG+AICH+DTS+W P+HGAF+ALGS PGR+EVCHWIFENDM WA+AD
Sbjct: 573 LNSKKKINHGIAICHMDTSSWGPSHGAFLALGSKPGRIEVCHWIFENDMNWAIAD 626

BLAST of Spg025633 vs. ExPASy Swiss-Prot
Match: P92982 (Polygalacturonase 1 beta-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=PGL1 PE=2 SV=2)

HSP 1 Score: 787.3 bits (2032), Expect = 1.2e-226
Identity = 385/593 (64.92%), Postives = 460/593 (77.57%), Query Frame = 0

Query: 32  NPFTPKAFLNRYWNKEVRNDLPKPSFLFSKVSPLTAVQSATFAKLAAENALSSRLPEFCS 91
           NPFTPKA L RYWNK++ +  P  +FL SK SPLTAV SATFAKLA+ENAL  RLP+FCS
Sbjct: 36  NPFTPKASLVRYWNKQIHHQSPGSAFLLSKASPLTAVASATFAKLASENALPDRLPDFCS 95

Query: 92  AAHLLCFPDLSPSLAKHDRDSNFAVYSNRNFTTYGTQRSGGIDSFKNYSNDDNVIVDSFR 151
           AA+L CFPDL P+L KH  D  F+VY  RNFT YGT   GG DSFK YS + N + DSFR
Sbjct: 96  AANLFCFPDLGPTLEKHSNDVKFSVYDQRNFTNYGTIHPGGADSFKKYSQNGNAVTDSFR 155

Query: 152 RYSHEALGHKDQFTTYATGTNVPDQSFNTYGSKAVGGDGKFTTYEESVNVPNLRFTTYST 211
           RYS +A GH+D+FT YA  +NV ++ FN+YG+ A GG G F  Y+  VN P  RF  YS 
Sbjct: 156 RYSRDAAGHEDKFTGYADNSNVVEEKFNSYGTSATGGSGDFKNYQSGVNNPTTRFKAYSD 215

Query: 212 DATGRSHTFKSYSENANAG-DQSFANYGKRGNGGPNEFAGYGTNANVVGSGFSSYGSDGN 271
           +  GR+ TFK+Y+  AN+G  QSF +YGK GNG PNEF+ YG ++NV+GS FS+YG +GN
Sbjct: 216 EGNGRAQTFKTYTHEANSGPGQSFTSYGKNGNGAPNEFSSYGVSSNVIGSDFSNYGENGN 275

Query: 272 GANDTFTNYGNEQNNPQNNFRSYGESGNGAIESFSSYRDQANVGDDSFQSYSKNSNSAKV 331
            ANDTF  YG + N PQNNF+SYG SGN A+++F +YRD ANVGDDSF SY+KNSN  KV
Sbjct: 276 AANDTFKGYGGDGNVPQNNFKSYGASGNAAVDTFLNYRDNANVGDDSFSSYAKNSNFEKV 335

Query: 332 NFANYGKSFNEGTDKFSGYGQNANGQSVGFKIYGVNTTFKDYAKQGISFAKYSNVSSGGG 391
           +F NYGKS N G++ FSGYG+ A G  + FK Y  N+TFKDY K G+ FAKY+  S GGG
Sbjct: 336 DFVNYGKSINPGSESFSGYGKGAEGNKIDFKTYTQNSTFKDYTKTGVEFAKYNRSSLGGG 395

Query: 392 KMAVSGSLAKRWVEPGKFFRESMLKKGKVMAMPDIRDKMPKRSFLPRSIVSKLPFSNSKV 451
           K         +WVEPGKFFRESMLK+G ++ MPDI+DKMPKRSFLPRSIVSKLPFS SK+
Sbjct: 396 K------TVNKWVEPGKFFRESMLKEGTLIWMPDIKDKMPKRSFLPRSIVSKLPFSTSKI 455

Query: 452 GVMKEIFHAGENSSMETMMTETLRECERAPSAGETKRCVGSVEDMIDFATSVLGRNVVVR 511
             +K +FHA +NS+ME ++T+ +RECER P+  ETKRCVGS EDMIDFATSVLGR+VV+R
Sbjct: 456 AEIKRVFHANDNSTMEGIITDAVRECERPPTVSETKRCVGSAEDMIDFATSVLGRSVVLR 515

Query: 512 TTENTKGSKGNVMIGAVKGINGGKVTQSVSCHQSLFPYLLYYCHSVPKVRVYEADLLDPS 571
           TTE+  GSK  VMIG V GINGG+VT+SVSCHQSL+PYLLYYCHSVPKVRVYE+DLLDP 
Sbjct: 516 TTESVAGSKEKVMIGKVNGINGGRVTKSVSCHQSLYPYLLYYCHSVPKVRVYESDLLDPK 575

Query: 572 SKATINHGVAICHLDTSAWSPAHGAFIALGSAPGRVEVCHWIFENDMTWAVAD 624
           SKA INHG+AICH+DTSAW   HGAF+ LGS PG++EVCHWIFENDM WA+AD
Sbjct: 576 SKAKINHGIAICHMDTSAWGANHGAFMLLGSRPGQIEVCHWIFENDMNWAIAD 622

BLAST of Spg025633 vs. ExPASy Swiss-Prot
Match: Q40161 (Polygalacturonase-1 non-catalytic subunit beta OS=Solanum lycopersicum OX=4081 GN=GP1 PE=1 SV=1)

HSP 1 Score: 752.3 bits (1941), Expect = 4.4e-216
Identity = 380/620 (61.29%), Postives = 463/620 (74.68%), Query Frame = 0

Query: 12  LSLFIFLLSPLNVSAAGDQD--NPFTPKAFLNRYWNKEVRNDLPKPSFLFSKVSPLTAVQ 71
           L L +F L   NV   GD +  NPFTPK +L RYW K++ NDLPKP FL +K SPL A Q
Sbjct: 12  LLLLLFSLPSFNVVVGGDGESGNPFTPKGYLIRYWKKQISNDLPKPWFLLNKASPLNAAQ 71

Query: 72  SATFAKLAA-ENALSSRLPEFCSAAHLLCFPDLSPSLAKHDRDSNFAVYSNRNFTTYGTQ 131
            AT+ KL A +NAL+++L  FCS+A+L+C PDLSPSL KH  D +FA YS++NFT YGT 
Sbjct: 72  YATYTKLVADQNALTTQLHTFCSSANLMCAPDLSPSLEKHSGDIHFATYSDKNFTNYGTN 131

Query: 132 RSG-GIDSFKNYSNDDNVIVDSFRRYSHEALGHKDQFTTYATGTNVPDQSFNTYGSKAVG 191
             G G+++FKNYS  +N+ V+SFRRY   +    ++F  YA+  NV DQSFN+Y +   G
Sbjct: 132 EPGIGVNTFKNYSEGENIPVNSFRRYGRGS-PRDNKFDNYASDGNVIDQSFNSYSTSTAG 191

Query: 192 GDGKFTTYEESVNVPNLRFTTYSTDATGRSHTFKSYSENANAGDQSFANYGKRGNGGPNE 251
           G GKFT Y  + N PNL FT+YS   TG    F  YS+ ANAGDQ F +YGK GNG   E
Sbjct: 192 GSGKFTNYAANANDPNLHFTSYSDQGTGGVQKFTIYSQEANAGDQYFKSYGKNGNGANGE 251

Query: 252 FAGYGTNANVVGSGFSSYGSDGNGANDTFTNYGNEQNNPQNNFRSYGESGNGAIESFSSY 311
           F  YG + NV+GS F++YG   NG +  FT+YG   N P+N+F +YG  GNG  E+F+SY
Sbjct: 252 FVSYGNDTNVIGSTFTNYGQTANGGDQKFTSYGFNGNVPENHFTNYGAGGNGPSETFNSY 311

Query: 312 RDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNANGQSVGFKIYGVNT 371
           RDQ+NVGDD+F +Y K++N  + NF NYG+SFNEGTD F+ YG+  N   + FK YGVN 
Sbjct: 312 RDQSNVGDDTFTTYVKDANGGEANFTNYGQSFNEGTDVFTTYGKGGNDPHINFKTYGVNN 371

Query: 372 TFKDYAKQGISFAKYSNVSSG--GGKMAVSG--SLAKRWVEPGKFFRESMLKKGKVMAMP 431
           TFKDY K   +F+ Y N +S      M V+G   +  RWVEPGKFFRE MLK G +M MP
Sbjct: 372 TFKDYVKDTATFSNYHNKTSQVLASLMEVNGGKKVNNRWVEPGKFFREKMLKSGTIMPMP 431

Query: 432 DIRDKMPKRSFLPRSIVSKLPFSNSKVGVMKEIFHAGENSSMETMMTETLRECERAPSAG 491
           DI+DKMPKRSFLPR I SKLPFS SK+  +K+IFHAG+ S +E M+ + L ECERAPSAG
Sbjct: 432 DIKDKMPKRSFLPRVIASKLPFSTSKIAELKKIFHAGDESQVEKMIGDALSECERAPSAG 491

Query: 492 ETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNVMIGAVKGINGGKVTQSVSCHQ 551
           ETKRCV S EDMIDFATSVLGRNVVVRTTE+TKGS GN+MIG+VKGINGGKVT+SVSCHQ
Sbjct: 492 ETKRCVNSAEDMIDFATSVLGRNVVVRTTEDTKGSNGNIMIGSVKGINGGKVTKSVSCHQ 551

Query: 552 SLFPYLLYYCHSVPKVRVYEADLLDPSSKATINHGVAICHLDTSAWSPAHGAFIALGSAP 611
           +L+PYLLYYCHSVPKVRVYEAD+LDP+SK  INHGVAICH+DTS+W P+HGAF+ALGS P
Sbjct: 552 TLYPYLLYYCHSVPKVRVYEADILDPNSKVKINHGVAICHVDTSSWGPSHGAFVALGSGP 611

Query: 612 GRVEVCHWIFENDMTWAVAD 624
           G++EVCHWIFENDMTWA+AD
Sbjct: 612 GKIEVCHWIFENDMTWAIAD 630

BLAST of Spg025633 vs. ExPASy Swiss-Prot
Match: O80760 (Polygalacturonase 1 beta-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=PGL2 PE=2 SV=1)

HSP 1 Score: 749.6 bits (1934), Expect = 2.9e-215
Identity = 381/619 (61.55%), Postives = 447/619 (72.21%), Query Frame = 0

Query: 15  FIFLLSPLNVSAAG---------DQDNPFTPKAFLNRYWNKEVRNDLPKPSFLFSKVSPL 74
           F  + S  NV  AG           +NPFTPKA L RYWN  +  D PKPSF  SK SPL
Sbjct: 12  FFCIFSSSNVHFAGAKQTAGNITPSENPFTPKASLIRYWNNHINGDSPKPSFFLSKASPL 71

Query: 75  TAVQSATFAKLAAENALSSRLPEFCSAAHLLCFPDLSP-SLAKHDRDSNFAVYSNRNFTT 134
           TAV S  FA LA+ +AL++   +FCSAA L CFP+L+  SL KH  D NFA YS +NFT 
Sbjct: 72  TAVDSTRFASLASNHALNTHHSDFCSAAKLFCFPELAAHSLEKHGDDVNFAAYSGKNFTN 131

Query: 135 YGTQRSGGIDSFKNYSNDDNVIVDSFRRYSHEALGHKDQFTTYATGTNVPDQSFNTYGSK 194
           YG+ R  G DSFKNYS  DN+ VDSFRRYS  + GH D FT YA   NV DQSF TY + 
Sbjct: 132 YGSDRLSGADSFKNYSGGDNIAVDSFRRYSRNSAGHDDGFTNYAGEVNVADQSFTTYATG 191

Query: 195 AVGGDGKFTTYEESVNVPNLRFTTYSTDATGRSHTFKSYSENANAGDQSFANYGKRGNGG 254
             GG G+FT Y    N PN RFT+YS  A GRS TF +YSEN N G QSF +Y K GNG 
Sbjct: 192 TTGGSGEFTNYNTDANEPNGRFTSYSDKANGRSQTFTTYSENGNTGYQSFTSYSKNGNGA 251

Query: 255 PNEFAGYGTNANVVGSGFSSYGSDGNGANDTFTNYGNEQNNPQNNFRSYGESGNGAIESF 314
           PNEF+GYGT +NVV +GF+ YG   NGAND+FT+YG   N P N F+ YG+ GNGA+  F
Sbjct: 252 PNEFSGYGTGSNVVNTGFTKYGESANGANDSFTSYGENGNVPVNEFKGYGDGGNGAVYGF 311

Query: 315 SSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNANGQSVGFKIYG 374
            +YRDQ+N+G DSF SY+KNSN+ KVNF NYGKSFN G+D F+GYGQ+  G +V FK YG
Sbjct: 312 KNYRDQSNIGVDSFSSYAKNSNNEKVNFVNYGKSFNLGSDNFTGYGQDNVGGNVSFKTYG 371

Query: 375 VNTTFKDYAKQGISFAKYSNVSSGGGKMAVSGSLAKRWVEPGKFFRESMLKKGKVMAMPD 434
              +FK Y K G+ FA+YSN  S  GK         +WVE GKFFRE+MLK+G +M MPD
Sbjct: 372 QGQSFKVYTKDGVVFARYSNNVSSNGK------TVNKWVEEGKFFREAMLKEGTLMQMPD 431

Query: 435 IRDKMPKRSFLPRSIVSKLPFSNSKVGVMKEIFHAGENSSMETMMTETLRECERAPSAGE 494
           I+DKMPKR+FLPR+IV  LPFS+S +G +  +F AGENSSM  +++  + ECER  S GE
Sbjct: 432 IKDKMPKRTFLPRNIVKNLPFSSSTIGEIWRVFGAGENSSMAGIISSAVSECERPASHGE 491

Query: 495 TKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNVMIGAVKGINGGKVTQSVSCHQS 554
           TKRCVGS EDMIDFATSVLGR VVVRTTEN  GSK  V+IG V GINGG VT++VSCHQS
Sbjct: 492 TKRCVGSAEDMIDFATSVLGRGVVVRTTENVVGSKKKVVIGKVNGINGGDVTRAVSCHQS 551

Query: 555 LFPYLLYYCHSVPKVRVYEADLLDPSSKATINHGVAICHLDTSAWSPAHGAFIALGSAPG 614
           L+PYLLYYCHSVP+VRVYE DLLDP S   INHGVAICH+DTSAWSP+HGAF+ALGS PG
Sbjct: 552 LYPYLLYYCHSVPRVRVYETDLLDPKSLEKINHGVAICHIDTSAWSPSHGAFLALGSGPG 611

Query: 615 RVEVCHWIFENDMTWAVAD 624
           ++EVCHWIFENDMTW + D
Sbjct: 612 QIEVCHWIFENDMTWNIID 624

BLAST of Spg025633 vs. ExPASy Swiss-Prot
Match: P93218 (Polygalacturonase non-catalytic subunit AroGP3 OS=Solanum lycopersicum OX=4081 GN=GP3 PE=3 SV=1)

HSP 1 Score: 733.0 bits (1891), Expect = 2.8e-210
Identity = 376/627 (59.97%), Postives = 456/627 (72.73%), Query Frame = 0

Query: 12  LSLFIFLLSPLNVSAAGDQD---NPFTPKAFLNRYWNKEVRNDLPKPSFLFSKVSPLTAV 71
           L L +F LS L+V  A D D   NPFTPK +L RYWNK V NDLPKP FL +K SPL A 
Sbjct: 12  LLLLLFTLSSLDVVVAKDGDESGNPFTPKGYLIRYWNKHVSNDLPKPWFLLNKASPLNAA 71

Query: 72  QSATFAKLAA-ENALSSRLPEFCSAAHLLCFPDLSPSLAKHDRDSNFAVYSNRNFTTYGT 131
           Q AT+ KL A +NA ++ L  FCS+A+L+C  DL PSL KH  D +F  Y ++NFT YGT
Sbjct: 72  QYATYTKLVADQNAFTTHLQSFCSSANLMCALDLLPSLEKHKGDVHFVSYGDKNFTNYGT 131

Query: 132 QRSG-GIDSFKNYSNDDNVIVDSFRRYSHEALGHKDQFTTY-ATGTNVPDQSFNTYGSKA 191
           + SG G +SFKNY+ ++N  V+SFRRY  ++  H +QF  Y A G N P QSFN+Y +  
Sbjct: 132 KESGLGFNSFKNYTEEENFPVNSFRRYGRDS-PHDNQFDNYGAPGGNTPVQSFNSYSTNT 191

Query: 192 VGGDGKFTTYEESVNVPNLRFTTYSTDATGRSHTFKSYSENANAGDQSFANYGKRGNGGP 251
            GG G+FT Y    NVP+L FT+YS   TG    FKSY  + NAG QSF +YGK GNG  
Sbjct: 192 PGGSGQFTNYAPGSNVPDLHFTSYSDHGTGGEQDFKSYGNDGNAGQQSFKSYGKDGNGAD 251

Query: 252 NEFAGYGTNANVVGSGFSSYGSDGNGANDTFTNYGNEQNNPQNNFRSYGESGNGAIESFS 311
           ++F  YG N NV GS F++YG   NG N  FT+YG   NNP ++F +YG  GNG  E+F+
Sbjct: 252 SQFTTYGNNTNVDGSDFTNYGEAANGENQNFTSYGFNGNNPASSFNNYGVGGNGPSEAFN 311

Query: 312 SYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNANGQSVGFKIYGV 371
           SYRDQ+NVGDD+F +Y K++N  + NF NYG+SFNEGTD F+ YG+  N   + FK YGV
Sbjct: 312 SYRDQSNVGDDTFTTYVKDANGGEANFTNYGQSFNEGTDVFTTYGKGGNDPHINFKTYGV 371

Query: 372 NTTFKDYAKQGISFAKYSNVSS---------GGGKMAVSGSLAKRWVEPGKFFRESMLKK 431
           N TFKDY K   +F+ Y N +S          GGK      +  RW+EPGKFFRE MLK 
Sbjct: 372 NNTFKDYVKDTATFSNYHNKTSQDLASLSEVNGGK-----KVNNRWIEPGKFFREKMLKS 431

Query: 432 GKVMAMPDIRDKMPKRSFLPRSIVSKLPFSNSKVGVMKEIFHAGENSSMETMMTETLREC 491
           G +M MPDI+DKMPKRSFLPR+I +KLPFS SK+  +K+IFHA  +S +  M+ + L EC
Sbjct: 432 GTIMPMPDIKDKMPKRSFLPRAIAAKLPFSTSKIDELKKIFHAANDSQVAKMIGDALSEC 491

Query: 492 ERAPSAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNVMIGAVKGINGGKVT 551
           ERAPS GETK+CV S EDMIDFATSVLGRNVVVRTTENT GSKGN+MIG++KGINGGKVT
Sbjct: 492 ERAPSPGETKQCVNSAEDMIDFATSVLGRNVVVRTTENTNGSKGNIMIGSIKGINGGKVT 551

Query: 552 QSVSCHQSLFPYLLYYCHSVPKVRVYEADLLDPSSKATINHGVAICHLDTSAWSPAHGAF 611
           +SVSCHQ+L+P LLYYCHSVPKVRVYEAD+LDP+SKA INHGVAICH+DTS+W P HGAF
Sbjct: 552 KSVSCHQTLYPSLLYYCHSVPKVRVYEADILDPNSKAKINHGVAICHVDTSSWGPRHGAF 611

Query: 612 IALGSAPGRVEVCHWIFENDMTWAVAD 624
           +ALGS PG++EVCHWIFENDMTWA AD
Sbjct: 612 VALGSGPGKIEVCHWIFENDMTWATAD 632

BLAST of Spg025633 vs. ExPASy TrEMBL
Match: A0A6J1HB03 (polygalacturonase 1 beta-like protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111462438 PE=4 SV=1)

HSP 1 Score: 1134.4 bits (2933), Expect = 0.0e+00
Identity = 567/623 (91.01%), Postives = 596/623 (95.67%), Query Frame = 0

Query: 1   MRRLPAPLVVPLSLFIFLLSPLNVSAAGDQDNPFTPKAFLNRYWNKEVRNDLPKPSFLFS 60
           M  LPAPL+V L LFIFLLSPLNV AA D+DNPFTPKAFLNRYWNKEVRNDLPKP+FLFS
Sbjct: 1   MHTLPAPLLVLLYLFIFLLSPLNVPAAADEDNPFTPKAFLNRYWNKEVRNDLPKPTFLFS 60

Query: 61  KVSPLTAVQSATFAKLAAENALSSRLPEFCSAAHLLCFPDLSPSLAKHDRDSNFAVYSNR 120
           KVSPLTAVQSA FAKLAA+NALSSRLPEFCSAA+LLCFPDLSPSLAKHD  SNFAVYSN+
Sbjct: 61  KVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHDSTSNFAVYSNK 120

Query: 121 NFTTYGTQRSGGIDSFKNYSNDDNVIVDSFRRYSHEALGHKDQFTTYATGTNVPDQSFNT 180
           NFTTYGTQRSGGIDSFKNYSN DNV+VDSFRRYSHEALGH DQF+TY T TNVPDQSFNT
Sbjct: 121 NFTTYGTQRSGGIDSFKNYSNGDNVVVDSFRRYSHEALGHTDQFSTYGTDTNVPDQSFNT 180

Query: 181 YGSKAVGGDGKFTTYEESVNVPNLRFTTYSTDATGRSHTFKSYSENANAGDQSFANYGKR 240
           Y SKAVGG+G FTTYE+SVNVPNLRFTTYS+DATGRS +FKSYSENANAGDQSF NYGKR
Sbjct: 181 YASKAVGGNGGFTTYEQSVNVPNLRFTTYSSDATGRSQSFKSYSENANAGDQSFVNYGKR 240

Query: 241 GNGGPNEFAGYGTNANVVGSGFSSYGSDGNGANDTFTNYGNEQNNPQNNFRSYGESGNGA 300
           GNGGPNEF GYGTNANV+GSGFSSYG++GNGANDTFTNYGNEQNNPQNNFRSYG+ GNGA
Sbjct: 241 GNGGPNEFTGYGTNANVIGSGFSSYGANGNGANDTFTNYGNEQNNPQNNFRSYGDGGNGA 300

Query: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNANGQSVGF 360
           IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQN NGQSVGF
Sbjct: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNGNGQSVGF 360

Query: 361 KIYGVNTTFKDYAKQGISFAKYSNVSSGGGKMAVSGSLAKRWVEPGKFFRESMLKKGKVM 420
           K YGVNTTFKDYAKQGISFAKYSNVSSGG KM+VS SLAKRWVEPGKFFRESML+KG VM
Sbjct: 361 KTYGVNTTFKDYAKQGISFAKYSNVSSGGAKMSVSESLAKRWVEPGKFFRESMLRKGTVM 420

Query: 421 AMPDIRDKMPKRSFLPRSIVSKLPFSNSKVGVMKEIFHAGENSSMETMMTETLRECERAP 480
           AMPDIRDKMPKRSFLPRSI+SKLPFS+SK+ VMK+IFHAG+NSSMETMMTETLR+CER P
Sbjct: 421 AMPDIRDKMPKRSFLPRSILSKLPFSSSKLEVMKQIFHAGDNSSMETMMTETLRDCERTP 480

Query: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNVMIGAVKGINGGKVTQSVS 540
           SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGN+MIGAVKGI GGKVT+SVS
Sbjct: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKGIEGGKVTESVS 540

Query: 541 CHQSLFPYLLYYCHSVPKVRVYEADLLDPSSKATINHGVAICHLDTSAWSPAHGAFIALG 600
           CHQSLFPYLLYYCHSVPKVRVYEA+LLDP+SKATINHGVAICHLDTSAWSPAHGAFIALG
Sbjct: 541 CHQSLFPYLLYYCHSVPKVRVYEAELLDPTSKATINHGVAICHLDTSAWSPAHGAFIALG 600

Query: 601 SAPGRVEVCHWIFENDMTWAVAD 624
           SAPGRVEVCHWIFENDMTW+VAD
Sbjct: 601 SAPGRVEVCHWIFENDMTWSVAD 623

BLAST of Spg025633 vs. ExPASy TrEMBL
Match: A0A5A7V5U8 (Polygalacturonase 1 beta-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G002300 PE=4 SV=1)

HSP 1 Score: 1133.6 bits (2931), Expect = 0.0e+00
Identity = 564/623 (90.53%), Postives = 593/623 (95.18%), Query Frame = 0

Query: 1   MRRLPAPLVVPLSLFIFLLSPLNVSAAGDQDNPFTPKAFLNRYWNKEVRNDLPKPSFLFS 60
           M RLP   VVPL LFIF LS LNV A GD+DNPFTPKAFLNRYWNKEVRND+PKPSFLFS
Sbjct: 1   MHRLPTLHVVPLYLFIFSLSALNVPAVGDEDNPFTPKAFLNRYWNKEVRNDIPKPSFLFS 60

Query: 61  KVSPLTAVQSATFAKLAAENALSSRLPEFCSAAHLLCFPDLSPSLAKHDRDSNFAVYSNR 120
           K SPLTAVQSA FAKLAAENALSSRLP+FCSAA+LLCFPDLSPSLAKHDRDSNFAVYSN+
Sbjct: 61  KASPLTAVQSAAFAKLAAENALSSRLPDFCSAANLLCFPDLSPSLAKHDRDSNFAVYSNK 120

Query: 121 NFTTYGTQRSGGIDSFKNYSNDDNVIVDSFRRYSHEALGHKDQFTTYATGTNVPDQSFNT 180
           NFTTYGTQRSGGIDSFKNYSN DNV+VDSFRRYSHEALGH D+F+TY T TNVPDQSFNT
Sbjct: 121 NFTTYGTQRSGGIDSFKNYSNGDNVVVDSFRRYSHEALGHTDRFSTYGTETNVPDQSFNT 180

Query: 181 YGSKAVGGDGKFTTYEESVNVPNLRFTTYSTDATGRSHTFKSYSENANAGDQSFANYGKR 240
           Y SKA+GGDGKFTTYE+SVNVPNLRFTTYS+DATGRS  FKSYSENANAGDQSF NYGKR
Sbjct: 181 YASKAIGGDGKFTTYEQSVNVPNLRFTTYSSDATGRSQAFKSYSENANAGDQSFVNYGKR 240

Query: 241 GNGGPNEFAGYGTNANVVGSGFSSYGSDGNGANDTFTNYGNEQNNPQNNFRSYGESGNGA 300
           GNGGPNEF GYGT+ANVVGS FSSYG+DGNGANDTFTNYGN+QNNPQNNFRSYGE GNGA
Sbjct: 241 GNGGPNEFTGYGTSANVVGSRFSSYGTDGNGANDTFTNYGNDQNNPQNNFRSYGEGGNGA 300

Query: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNANGQSVGF 360
           IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFA+YGKSFNEGTDKFSGYGQN NGQSVGF
Sbjct: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFASYGKSFNEGTDKFSGYGQNGNGQSVGF 360

Query: 361 KIYGVNTTFKDYAKQGISFAKYSNVSSGGGKMAVSGSLAKRWVEPGKFFRESMLKKGKVM 420
           K+YGVNTTFKDYAKQGI+FAKYSNVSSGG K +VSGSLAKRWVEPGKFFRESML+KG VM
Sbjct: 361 KVYGVNTTFKDYAKQGINFAKYSNVSSGGAKTSVSGSLAKRWVEPGKFFRESMLRKGSVM 420

Query: 421 AMPDIRDKMPKRSFLPRSIVSKLPFSNSKVGVMKEIFHAGENSSMETMMTETLRECERAP 480
           AMPDIRDKMPKRSFLPRSI+SKLPFS+SK+GVMK+IFHAG+NSSMETMMTET+RECER P
Sbjct: 421 AMPDIRDKMPKRSFLPRSILSKLPFSSSKLGVMKQIFHAGDNSSMETMMTETVRECERVP 480

Query: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNVMIGAVKGINGGKVTQSVS 540
           SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGN+MIG VKG+NGGKVT+SVS
Sbjct: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGKVKGMNGGKVTESVS 540

Query: 541 CHQSLFPYLLYYCHSVPKVRVYEADLLDPSSKATINHGVAICHLDTSAWSPAHGAFIALG 600
           CHQSLFPYLLYYCHSVPKVRVYE DLLDP+SKATINHGVAICHLDTSAWSPAHGAFIALG
Sbjct: 541 CHQSLFPYLLYYCHSVPKVRVYEVDLLDPASKATINHGVAICHLDTSAWSPAHGAFIALG 600

Query: 601 SAPGRVEVCHWIFENDMTWAVAD 624
           SAPGRVEVCHWIFENDMTW VAD
Sbjct: 601 SAPGRVEVCHWIFENDMTWTVAD 623

BLAST of Spg025633 vs. ExPASy TrEMBL
Match: A0A1S3B9L4 (polygalacturonase 1 beta-like protein 3 OS=Cucumis melo OX=3656 GN=LOC103487686 PE=4 SV=1)

HSP 1 Score: 1133.6 bits (2931), Expect = 0.0e+00
Identity = 564/623 (90.53%), Postives = 593/623 (95.18%), Query Frame = 0

Query: 1   MRRLPAPLVVPLSLFIFLLSPLNVSAAGDQDNPFTPKAFLNRYWNKEVRNDLPKPSFLFS 60
           M RLP   VVPL LFIF LS LNV A GD+DNPFTPKAFLNRYWNKEVRND+PKPSFLFS
Sbjct: 1   MHRLPTLHVVPLYLFIFSLSALNVPAVGDEDNPFTPKAFLNRYWNKEVRNDIPKPSFLFS 60

Query: 61  KVSPLTAVQSATFAKLAAENALSSRLPEFCSAAHLLCFPDLSPSLAKHDRDSNFAVYSNR 120
           K SPLTAVQSA FAKLAAENALSSRLP+FCSAA+LLCFPDLSPSLAKHDRDSNFAVYSN+
Sbjct: 61  KASPLTAVQSAAFAKLAAENALSSRLPDFCSAANLLCFPDLSPSLAKHDRDSNFAVYSNK 120

Query: 121 NFTTYGTQRSGGIDSFKNYSNDDNVIVDSFRRYSHEALGHKDQFTTYATGTNVPDQSFNT 180
           NFTTYGTQRSGGIDSFKNYSN DNV+VDSFRRYSHEALGH D+F+TY T TNVPDQSFNT
Sbjct: 121 NFTTYGTQRSGGIDSFKNYSNGDNVVVDSFRRYSHEALGHTDRFSTYGTETNVPDQSFNT 180

Query: 181 YGSKAVGGDGKFTTYEESVNVPNLRFTTYSTDATGRSHTFKSYSENANAGDQSFANYGKR 240
           Y SKA+GGDGKFTTYE+SVNVPNLRFTTYS+DATGRS  FKSYSENANAGDQSF NYGKR
Sbjct: 181 YASKAIGGDGKFTTYEQSVNVPNLRFTTYSSDATGRSQAFKSYSENANAGDQSFVNYGKR 240

Query: 241 GNGGPNEFAGYGTNANVVGSGFSSYGSDGNGANDTFTNYGNEQNNPQNNFRSYGESGNGA 300
           GNGGPNEF GYGT+ANVVGS FSSYG+DGNGANDTFTNYGN+QNNPQNNFRSYGE GNGA
Sbjct: 241 GNGGPNEFTGYGTSANVVGSRFSSYGTDGNGANDTFTNYGNDQNNPQNNFRSYGEGGNGA 300

Query: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNANGQSVGF 360
           IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFA+YGKSFNEGTDKFSGYGQN NGQSVGF
Sbjct: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFASYGKSFNEGTDKFSGYGQNGNGQSVGF 360

Query: 361 KIYGVNTTFKDYAKQGISFAKYSNVSSGGGKMAVSGSLAKRWVEPGKFFRESMLKKGKVM 420
           K+YGVNTTFKDYAKQGI+FAKYSNVSSGG K +VSGSLAKRWVEPGKFFRESML+KG VM
Sbjct: 361 KVYGVNTTFKDYAKQGINFAKYSNVSSGGAKTSVSGSLAKRWVEPGKFFRESMLRKGSVM 420

Query: 421 AMPDIRDKMPKRSFLPRSIVSKLPFSNSKVGVMKEIFHAGENSSMETMMTETLRECERAP 480
           AMPDIRDKMPKRSFLPRSI+SKLPFS+SK+GVMK+IFHAG+NSSMETMMTET+RECER P
Sbjct: 421 AMPDIRDKMPKRSFLPRSILSKLPFSSSKLGVMKQIFHAGDNSSMETMMTETVRECERVP 480

Query: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNVMIGAVKGINGGKVTQSVS 540
           SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGN+MIG VKG+NGGKVT+SVS
Sbjct: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGKVKGMNGGKVTESVS 540

Query: 541 CHQSLFPYLLYYCHSVPKVRVYEADLLDPSSKATINHGVAICHLDTSAWSPAHGAFIALG 600
           CHQSLFPYLLYYCHSVPKVRVYE DLLDP+SKATINHGVAICHLDTSAWSPAHGAFIALG
Sbjct: 541 CHQSLFPYLLYYCHSVPKVRVYEVDLLDPASKATINHGVAICHLDTSAWSPAHGAFIALG 600

Query: 601 SAPGRVEVCHWIFENDMTWAVAD 624
           SAPGRVEVCHWIFENDMTW VAD
Sbjct: 601 SAPGRVEVCHWIFENDMTWTVAD 623

BLAST of Spg025633 vs. ExPASy TrEMBL
Match: A0A6J1K271 (polygalacturonase 1 beta-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111491752 PE=4 SV=1)

HSP 1 Score: 1129.8 bits (2921), Expect = 0.0e+00
Identity = 564/623 (90.53%), Postives = 595/623 (95.51%), Query Frame = 0

Query: 1   MRRLPAPLVVPLSLFIFLLSPLNVSAAGDQDNPFTPKAFLNRYWNKEVRNDLPKPSFLFS 60
           M  LPAPL+V L LFIFLLSPLNV AA D+DNPFTPKAFLNRYWNKEVRND+PKP+FLFS
Sbjct: 1   MHTLPAPLLVLLYLFIFLLSPLNVPAAADEDNPFTPKAFLNRYWNKEVRNDMPKPTFLFS 60

Query: 61  KVSPLTAVQSATFAKLAAENALSSRLPEFCSAAHLLCFPDLSPSLAKHDRDSNFAVYSNR 120
           KVSPLTAVQSA FAKLAA+NALSSRLPEFCSAA+LLCFPDLSPSLAKHD  SNFAVYSN+
Sbjct: 61  KVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHDSTSNFAVYSNK 120

Query: 121 NFTTYGTQRSGGIDSFKNYSNDDNVIVDSFRRYSHEALGHKDQFTTYATGTNVPDQSFNT 180
           NFTTYGTQRSGGIDSFKNYSN DNV+VDSFRRYSHEALGH DQF+TY T TNVPDQSFNT
Sbjct: 121 NFTTYGTQRSGGIDSFKNYSNGDNVVVDSFRRYSHEALGHTDQFSTYGTDTNVPDQSFNT 180

Query: 181 YGSKAVGGDGKFTTYEESVNVPNLRFTTYSTDATGRSHTFKSYSENANAGDQSFANYGKR 240
           Y SKAVGG+G FTTYE+SVNVPNLRFTTYS+DATGRS +FKSYSENANAGDQSF NYGKR
Sbjct: 181 YASKAVGGNGGFTTYEQSVNVPNLRFTTYSSDATGRSQSFKSYSENANAGDQSFVNYGKR 240

Query: 241 GNGGPNEFAGYGTNANVVGSGFSSYGSDGNGANDTFTNYGNEQNNPQNNFRSYGESGNGA 300
           GNGGPNEF GYGTNANV+GSGFSSYG++GNGANDTFTNYGNEQNNPQNNFRSYG+ GNGA
Sbjct: 241 GNGGPNEFTGYGTNANVIGSGFSSYGANGNGANDTFTNYGNEQNNPQNNFRSYGDGGNGA 300

Query: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNANGQSVGF 360
           IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQN NGQSVGF
Sbjct: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNGNGQSVGF 360

Query: 361 KIYGVNTTFKDYAKQGISFAKYSNVSSGGGKMAVSGSLAKRWVEPGKFFRESMLKKGKVM 420
           K YGVNTTFKDYAKQGISFAKYSNVSSGG KM+VS SLAKRWVEPGKFFRESML+KG V+
Sbjct: 361 KTYGVNTTFKDYAKQGISFAKYSNVSSGGAKMSVSESLAKRWVEPGKFFRESMLRKGTVV 420

Query: 421 AMPDIRDKMPKRSFLPRSIVSKLPFSNSKVGVMKEIFHAGENSSMETMMTETLRECERAP 480
           AMPDIRDKMPKRSFLPRSI+SKLPFS+SK+ VMK+IFHAG+NSSMETMMTETLR+CER P
Sbjct: 421 AMPDIRDKMPKRSFLPRSILSKLPFSSSKLEVMKQIFHAGDNSSMETMMTETLRDCERTP 480

Query: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNVMIGAVKGINGGKVTQSVS 540
           SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGN+MIGAVKGI GGKVT+SVS
Sbjct: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKGIEGGKVTESVS 540

Query: 541 CHQSLFPYLLYYCHSVPKVRVYEADLLDPSSKATINHGVAICHLDTSAWSPAHGAFIALG 600
           CHQSLFPYLLYYCHSVPKVRVYEA+LLDP+SKA INHGVAICHLDTSAWSPAHGAFIALG
Sbjct: 541 CHQSLFPYLLYYCHSVPKVRVYEAELLDPTSKAIINHGVAICHLDTSAWSPAHGAFIALG 600

Query: 601 SAPGRVEVCHWIFENDMTWAVAD 624
           SAPGRVEVCHWIFENDMTW+VAD
Sbjct: 601 SAPGRVEVCHWIFENDMTWSVAD 623

BLAST of Spg025633 vs. ExPASy TrEMBL
Match: A0A0A0LK44 (BURP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G348230 PE=4 SV=1)

HSP 1 Score: 1127.9 bits (2916), Expect = 0.0e+00
Identity = 559/623 (89.73%), Postives = 593/623 (95.18%), Query Frame = 0

Query: 1   MRRLPAPLVVPLSLFIFLLSPLNVSAAGDQDNPFTPKAFLNRYWNKEVRNDLPKPSFLFS 60
           M RLP   VVPL LFIF LS LNVS AGD+D+PFTPKAFLNRYWNKEVRND PKPSFLFS
Sbjct: 1   MHRLPTLYVVPLYLFIFSLSSLNVSTAGDEDDPFTPKAFLNRYWNKEVRNDFPKPSFLFS 60

Query: 61  KVSPLTAVQSATFAKLAAENALSSRLPEFCSAAHLLCFPDLSPSLAKHDRDSNFAVYSNR 120
           K SPLTAVQSA FAKLAAENALSSRLP+FCSAA+LLCFPDLSPSLAKHDRDSNFAVYSN+
Sbjct: 61  KASPLTAVQSAAFAKLAAENALSSRLPDFCSAANLLCFPDLSPSLAKHDRDSNFAVYSNK 120

Query: 121 NFTTYGTQRSGGIDSFKNYSNDDNVIVDSFRRYSHEALGHKDQFTTYATGTNVPDQSFNT 180
           NFTTYGTQRSGGIDSFKNYSN DNV+VDSFRRYSHEALGH D+F+ Y T TNVPDQSFNT
Sbjct: 121 NFTTYGTQRSGGIDSFKNYSNGDNVVVDSFRRYSHEALGHTDRFSVYGTDTNVPDQSFNT 180

Query: 181 YGSKAVGGDGKFTTYEESVNVPNLRFTTYSTDATGRSHTFKSYSENANAGDQSFANYGKR 240
           Y SKA+GGDGKFTTYE+SVNVPNLRFTTYS+DATGRS +FKSYSENANAGDQSF NYGKR
Sbjct: 181 YASKAIGGDGKFTTYEQSVNVPNLRFTTYSSDATGRSQSFKSYSENANAGDQSFVNYGKR 240

Query: 241 GNGGPNEFAGYGTNANVVGSGFSSYGSDGNGANDTFTNYGNEQNNPQNNFRSYGESGNGA 300
           GNGGPNEF GYGT+ANV+GS FSSYG++GNGANDTFTNYGN+QNNPQNNFRSYGE GNGA
Sbjct: 241 GNGGPNEFTGYGTSANVIGSRFSSYGAEGNGANDTFTNYGNDQNNPQNNFRSYGEGGNGA 300

Query: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNANGQSVGF 360
           IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFA+YGKSFNEGTDKFSGYGQN NGQSVGF
Sbjct: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFASYGKSFNEGTDKFSGYGQNGNGQSVGF 360

Query: 361 KIYGVNTTFKDYAKQGISFAKYSNVSSGGGKMAVSGSLAKRWVEPGKFFRESMLKKGKVM 420
           K YGVNTTFKDYAKQGI+FAKYSNVSSGG KM+VSGSLAKRWVEPGKFFRESML+KG VM
Sbjct: 361 KTYGVNTTFKDYAKQGINFAKYSNVSSGGAKMSVSGSLAKRWVEPGKFFRESMLRKGSVM 420

Query: 421 AMPDIRDKMPKRSFLPRSIVSKLPFSNSKVGVMKEIFHAGENSSMETMMTETLRECERAP 480
           AMPDIRDKMPKRSFLPRSI+SKLPFS+SK+GVMK+IFHAG+NSSMETMMTET+RECER P
Sbjct: 421 AMPDIRDKMPKRSFLPRSILSKLPFSSSKLGVMKQIFHAGDNSSMETMMTETVRECERLP 480

Query: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNVMIGAVKGINGGKVTQSVS 540
           SAGETKRCVGSVEDMIDFATSVLGRNVVV+TTENTKGSKGN+MIG VKG+NGGKVT+SVS
Sbjct: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVQTTENTKGSKGNIMIGKVKGMNGGKVTESVS 540

Query: 541 CHQSLFPYLLYYCHSVPKVRVYEADLLDPSSKATINHGVAICHLDTSAWSPAHGAFIALG 600
           CHQSLFPYLLYYCHSVPKVRVYE DLLDP++KAT+NHGVAICHLDTSAWSPAHGAFIALG
Sbjct: 541 CHQSLFPYLLYYCHSVPKVRVYEVDLLDPATKATVNHGVAICHLDTSAWSPAHGAFIALG 600

Query: 601 SAPGRVEVCHWIFENDMTWAVAD 624
           SAPGRVEVCHWIFENDMTW VAD
Sbjct: 601 SAPGRVEVCHWIFENDMTWTVAD 623

BLAST of Spg025633 vs. TAIR 10
Match: AT1G70370.1 (polygalacturonase 2 )

HSP 1 Score: 796.6 bits (2056), Expect = 1.4e-230
Identity = 388/595 (65.21%), Postives = 470/595 (78.99%), Query Frame = 0

Query: 30  QDNPFTPKAFLNRYWNKEVRNDLPKPSFLFSKVSPLTAVQSATFAKLAAENALSSRLPEF 89
           ++NPFTPKA L RYWNKE+R   P+  FL SK SPL AV SATF+KLAA N+L +R P+F
Sbjct: 33  EENPFTPKASLVRYWNKEIRGQSPRSEFLISKASPLNAVDSATFSKLAAANSLPTRFPDF 92

Query: 90  CSAAHLLCFPDLSPSLAKHDRDSNFAVYSNRNFTTYGTQRSGGIDSFKNYSNDDNVIVDS 149
           CSAA+L CFPDL  SL KHD D  F+VY  +NFT YG  R+GG DSFKNYS D NV+ DS
Sbjct: 93  CSAANLFCFPDLGASLEKHDDDVKFSVYDQKNFTNYGNARAGGADSFKNYSKDGNVVTDS 152

Query: 150 FRRYSHEALGHKDQFTTYATGTNVPDQSFNTYGSKAVGGDGKFTTYEESVNVPNLRFTTY 209
           FRRYS  A GH D+FT Y   +NV ++ FN+YG+   GG G FT Y+ +VN P  RFT Y
Sbjct: 153 FRRYSRNAAGHDDKFTVYGENSNVVEEGFNSYGTFGTGGAGDFTNYQNNVNNPTSRFTAY 212

Query: 210 STDATGRSHTFKSYSENANAGD-QSFANYGKRGNGGPNEFAGYGTNANVVGSGFSSYGSD 269
           S    GRS TFK+Y+  ANAG+ QSF +YGK GNG PNEF  YG ++NV+GSGFS+YG  
Sbjct: 213 SDGGNGRSQTFKTYTHEANAGNGQSFTSYGKNGNGVPNEFTSYGVSSNVIGSGFSNYGES 272

Query: 270 GNGANDTFTNYGNEQNNPQNNFRSYGESGNGAIESFSSYRDQANVGDDSFQSYSKNSNSA 329
           GN ANDTFT+YG++ N PQNNF +YG SGN A+++F++YRD+ANVGDDSF SY+K+SNS 
Sbjct: 273 GNAANDTFTSYGSDGNVPQNNFNNYGASGNAAVDTFANYRDKANVGDDSFSSYAKDSNSE 332

Query: 330 KVNFANYGKSFNEGTDKFSGYGQNANGQSVGFKIYGVNTTFKDYAKQGISFAKYSNVSSG 389
           KVNF NYG+SFN G++ F+GYG+ A G  + FK Y  N+TFKDYAK+G++FAKY NVS+ 
Sbjct: 333 KVNFVNYGQSFNPGSETFTGYGKGAEGSKLSFKTYTPNSTFKDYAKKGVAFAKY-NVSTT 392

Query: 390 GGKMAVSGSLAKRWVEPGKFFRESMLKKGKVMAMPDIRDKMPKRSFLPRSIVSKLPFSNS 449
                  G    +W+EPGKFFRES LK+G V+ MPDI+DKMPKRSFLPRSI++KLPFS S
Sbjct: 393 TANTVGDGKTVNKWIEPGKFFRESSLKEGTVIPMPDIKDKMPKRSFLPRSIITKLPFSTS 452

Query: 450 KVGVMKEIFHAGENSSMETMMTETLRECERAPSAGETKRCVGSVEDMIDFATSVLGRNVV 509
           K+G +K IFHA ENS+M  ++T+ + ECER PS GETKRCVGS EDMIDFATSVLGR+VV
Sbjct: 453 KLGEIKRIFHAVENSTMGGIITDAVTECERPPSVGETKRCVGSAEDMIDFATSVLGRSVV 512

Query: 510 VRTTENTKGSKGNVMIGAVKGINGGKVTQSVSCHQSLFPYLLYYCHSVPKVRVYEADLLD 569
           +RTTEN  GSK  V+IG V GINGGK+T++VSCHQSL+PYLLYYCHSVPKVRVYEADLL+
Sbjct: 513 LRTTENVAGSKEKVVIGKVNGINGGKLTKAVSCHQSLYPYLLYYCHSVPKVRVYEADLLE 572

Query: 570 PSSKATINHGVAICHLDTSAWSPAHGAFIALGSAPGRVEVCHWIFENDMTWAVAD 624
            +SK  INHG+AICH+DTS+W P+HGAF+ALGS PGR+EVCHWIFENDM WA+AD
Sbjct: 573 LNSKKKINHGIAICHMDTSSWGPSHGAFLALGSKPGRIEVCHWIFENDMNWAIAD 626

BLAST of Spg025633 vs. TAIR 10
Match: AT1G70370.2 (polygalacturonase 2 )

HSP 1 Score: 796.6 bits (2056), Expect = 1.4e-230
Identity = 388/595 (65.21%), Postives = 470/595 (78.99%), Query Frame = 0

Query: 30  QDNPFTPKAFLNRYWNKEVRNDLPKPSFLFSKVSPLTAVQSATFAKLAAENALSSRLPEF 89
           ++NPFTPKA L RYWNKE+R   P+  FL SK SPL AV SATF+KLAA N+L +R P+F
Sbjct: 33  EENPFTPKASLVRYWNKEIRGQSPRSEFLISKASPLNAVDSATFSKLAAANSLPTRFPDF 92

Query: 90  CSAAHLLCFPDLSPSLAKHDRDSNFAVYSNRNFTTYGTQRSGGIDSFKNYSNDDNVIVDS 149
           CSAA+L CFPDL  SL KHD D  F+VY  +NFT YG  R+GG DSFKNYS D NV+ DS
Sbjct: 93  CSAANLFCFPDLGASLEKHDDDVKFSVYDQKNFTNYGNARAGGADSFKNYSKDGNVVTDS 152

Query: 150 FRRYSHEALGHKDQFTTYATGTNVPDQSFNTYGSKAVGGDGKFTTYEESVNVPNLRFTTY 209
           FRRYS  A GH D+FT Y   +NV ++ FN+YG+   GG G FT Y+ +VN P  RFT Y
Sbjct: 153 FRRYSRNAAGHDDKFTVYGENSNVVEEGFNSYGTFGTGGAGDFTNYQNNVNNPTSRFTAY 212

Query: 210 STDATGRSHTFKSYSENANAGD-QSFANYGKRGNGGPNEFAGYGTNANVVGSGFSSYGSD 269
           S    GRS TFK+Y+  ANAG+ QSF +YGK GNG PNEF  YG ++NV+GSGFS+YG  
Sbjct: 213 SDGGNGRSQTFKTYTHEANAGNGQSFTSYGKNGNGVPNEFTSYGVSSNVIGSGFSNYGES 272

Query: 270 GNGANDTFTNYGNEQNNPQNNFRSYGESGNGAIESFSSYRDQANVGDDSFQSYSKNSNSA 329
           GN ANDTFT+YG++ N PQNNF +YG SGN A+++F++YRD+ANVGDDSF SY+K+SNS 
Sbjct: 273 GNAANDTFTSYGSDGNVPQNNFNNYGASGNAAVDTFANYRDKANVGDDSFSSYAKDSNSE 332

Query: 330 KVNFANYGKSFNEGTDKFSGYGQNANGQSVGFKIYGVNTTFKDYAKQGISFAKYSNVSSG 389
           KVNF NYG+SFN G++ F+GYG+ A G  + FK Y  N+TFKDYAK+G++FAKY NVS+ 
Sbjct: 333 KVNFVNYGQSFNPGSETFTGYGKGAEGSKLSFKTYTPNSTFKDYAKKGVAFAKY-NVSTT 392

Query: 390 GGKMAVSGSLAKRWVEPGKFFRESMLKKGKVMAMPDIRDKMPKRSFLPRSIVSKLPFSNS 449
                  G    +W+EPGKFFRES LK+G V+ MPDI+DKMPKRSFLPRSI++KLPFS S
Sbjct: 393 TANTVGDGKTVNKWIEPGKFFRESSLKEGTVIPMPDIKDKMPKRSFLPRSIITKLPFSTS 452

Query: 450 KVGVMKEIFHAGENSSMETMMTETLRECERAPSAGETKRCVGSVEDMIDFATSVLGRNVV 509
           K+G +K IFHA ENS+M  ++T+ + ECER PS GETKRCVGS EDMIDFATSVLGR+VV
Sbjct: 453 KLGEIKRIFHAVENSTMGGIITDAVTECERPPSVGETKRCVGSAEDMIDFATSVLGRSVV 512

Query: 510 VRTTENTKGSKGNVMIGAVKGINGGKVTQSVSCHQSLFPYLLYYCHSVPKVRVYEADLLD 569
           +RTTEN  GSK  V+IG V GINGGK+T++VSCHQSL+PYLLYYCHSVPKVRVYEADLL+
Sbjct: 513 LRTTENVAGSKEKVVIGKVNGINGGKLTKAVSCHQSLYPYLLYYCHSVPKVRVYEADLLE 572

Query: 570 PSSKATINHGVAICHLDTSAWSPAHGAFIALGSAPGRVEVCHWIFENDMTWAVAD 624
            +SK  INHG+AICH+DTS+W P+HGAF+ALGS PGR+EVCHWIFENDM WA+AD
Sbjct: 573 LNSKKKINHGIAICHMDTSSWGPSHGAFLALGSKPGRIEVCHWIFENDMNWAIAD 626

BLAST of Spg025633 vs. TAIR 10
Match: AT1G23760.1 (BURP domain-containing protein )

HSP 1 Score: 787.3 bits (2032), Expect = 8.8e-228
Identity = 385/593 (64.92%), Postives = 460/593 (77.57%), Query Frame = 0

Query: 32  NPFTPKAFLNRYWNKEVRNDLPKPSFLFSKVSPLTAVQSATFAKLAAENALSSRLPEFCS 91
           NPFTPKA L RYWNK++ +  P  +FL SK SPLTAV SATFAKLA+ENAL  RLP+FCS
Sbjct: 36  NPFTPKASLVRYWNKQIHHQSPGSAFLLSKASPLTAVASATFAKLASENALPDRLPDFCS 95

Query: 92  AAHLLCFPDLSPSLAKHDRDSNFAVYSNRNFTTYGTQRSGGIDSFKNYSNDDNVIVDSFR 151
           AA+L CFPDL P+L KH  D  F+VY  RNFT YGT   GG DSFK YS + N + DSFR
Sbjct: 96  AANLFCFPDLGPTLEKHSNDVKFSVYDQRNFTNYGTIHPGGADSFKKYSQNGNAVTDSFR 155

Query: 152 RYSHEALGHKDQFTTYATGTNVPDQSFNTYGSKAVGGDGKFTTYEESVNVPNLRFTTYST 211
           RYS +A GH+D+FT YA  +NV ++ FN+YG+ A GG G F  Y+  VN P  RF  YS 
Sbjct: 156 RYSRDAAGHEDKFTGYADNSNVVEEKFNSYGTSATGGSGDFKNYQSGVNNPTTRFKAYSD 215

Query: 212 DATGRSHTFKSYSENANAG-DQSFANYGKRGNGGPNEFAGYGTNANVVGSGFSSYGSDGN 271
           +  GR+ TFK+Y+  AN+G  QSF +YGK GNG PNEF+ YG ++NV+GS FS+YG +GN
Sbjct: 216 EGNGRAQTFKTYTHEANSGPGQSFTSYGKNGNGAPNEFSSYGVSSNVIGSDFSNYGENGN 275

Query: 272 GANDTFTNYGNEQNNPQNNFRSYGESGNGAIESFSSYRDQANVGDDSFQSYSKNSNSAKV 331
            ANDTF  YG + N PQNNF+SYG SGN A+++F +YRD ANVGDDSF SY+KNSN  KV
Sbjct: 276 AANDTFKGYGGDGNVPQNNFKSYGASGNAAVDTFLNYRDNANVGDDSFSSYAKNSNFEKV 335

Query: 332 NFANYGKSFNEGTDKFSGYGQNANGQSVGFKIYGVNTTFKDYAKQGISFAKYSNVSSGGG 391
           +F NYGKS N G++ FSGYG+ A G  + FK Y  N+TFKDY K G+ FAKY+  S GGG
Sbjct: 336 DFVNYGKSINPGSESFSGYGKGAEGNKIDFKTYTQNSTFKDYTKTGVEFAKYNRSSLGGG 395

Query: 392 KMAVSGSLAKRWVEPGKFFRESMLKKGKVMAMPDIRDKMPKRSFLPRSIVSKLPFSNSKV 451
           K         +WVEPGKFFRESMLK+G ++ MPDI+DKMPKRSFLPRSIVSKLPFS SK+
Sbjct: 396 K------TVNKWVEPGKFFRESMLKEGTLIWMPDIKDKMPKRSFLPRSIVSKLPFSTSKI 455

Query: 452 GVMKEIFHAGENSSMETMMTETLRECERAPSAGETKRCVGSVEDMIDFATSVLGRNVVVR 511
             +K +FHA +NS+ME ++T+ +RECER P+  ETKRCVGS EDMIDFATSVLGR+VV+R
Sbjct: 456 AEIKRVFHANDNSTMEGIITDAVRECERPPTVSETKRCVGSAEDMIDFATSVLGRSVVLR 515

Query: 512 TTENTKGSKGNVMIGAVKGINGGKVTQSVSCHQSLFPYLLYYCHSVPKVRVYEADLLDPS 571
           TTE+  GSK  VMIG V GINGG+VT+SVSCHQSL+PYLLYYCHSVPKVRVYE+DLLDP 
Sbjct: 516 TTESVAGSKEKVMIGKVNGINGGRVTKSVSCHQSLYPYLLYYCHSVPKVRVYESDLLDPK 575

Query: 572 SKATINHGVAICHLDTSAWSPAHGAFIALGSAPGRVEVCHWIFENDMTWAVAD 624
           SKA INHG+AICH+DTSAW   HGAF+ LGS PG++EVCHWIFENDM WA+AD
Sbjct: 576 SKAKINHGIAICHMDTSAWGANHGAFMLLGSRPGQIEVCHWIFENDMNWAIAD 622

BLAST of Spg025633 vs. TAIR 10
Match: AT1G60390.1 (polygalacturonase 1 )

HSP 1 Score: 749.6 bits (1934), Expect = 2.0e-216
Identity = 381/619 (61.55%), Postives = 447/619 (72.21%), Query Frame = 0

Query: 15  FIFLLSPLNVSAAG---------DQDNPFTPKAFLNRYWNKEVRNDLPKPSFLFSKVSPL 74
           F  + S  NV  AG           +NPFTPKA L RYWN  +  D PKPSF  SK SPL
Sbjct: 12  FFCIFSSSNVHFAGAKQTAGNITPSENPFTPKASLIRYWNNHINGDSPKPSFFLSKASPL 71

Query: 75  TAVQSATFAKLAAENALSSRLPEFCSAAHLLCFPDLSP-SLAKHDRDSNFAVYSNRNFTT 134
           TAV S  FA LA+ +AL++   +FCSAA L CFP+L+  SL KH  D NFA YS +NFT 
Sbjct: 72  TAVDSTRFASLASNHALNTHHSDFCSAAKLFCFPELAAHSLEKHGDDVNFAAYSGKNFTN 131

Query: 135 YGTQRSGGIDSFKNYSNDDNVIVDSFRRYSHEALGHKDQFTTYATGTNVPDQSFNTYGSK 194
           YG+ R  G DSFKNYS  DN+ VDSFRRYS  + GH D FT YA   NV DQSF TY + 
Sbjct: 132 YGSDRLSGADSFKNYSGGDNIAVDSFRRYSRNSAGHDDGFTNYAGEVNVADQSFTTYATG 191

Query: 195 AVGGDGKFTTYEESVNVPNLRFTTYSTDATGRSHTFKSYSENANAGDQSFANYGKRGNGG 254
             GG G+FT Y    N PN RFT+YS  A GRS TF +YSEN N G QSF +Y K GNG 
Sbjct: 192 TTGGSGEFTNYNTDANEPNGRFTSYSDKANGRSQTFTTYSENGNTGYQSFTSYSKNGNGA 251

Query: 255 PNEFAGYGTNANVVGSGFSSYGSDGNGANDTFTNYGNEQNNPQNNFRSYGESGNGAIESF 314
           PNEF+GYGT +NVV +GF+ YG   NGAND+FT+YG   N P N F+ YG+ GNGA+  F
Sbjct: 252 PNEFSGYGTGSNVVNTGFTKYGESANGANDSFTSYGENGNVPVNEFKGYGDGGNGAVYGF 311

Query: 315 SSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNANGQSVGFKIYG 374
            +YRDQ+N+G DSF SY+KNSN+ KVNF NYGKSFN G+D F+GYGQ+  G +V FK YG
Sbjct: 312 KNYRDQSNIGVDSFSSYAKNSNNEKVNFVNYGKSFNLGSDNFTGYGQDNVGGNVSFKTYG 371

Query: 375 VNTTFKDYAKQGISFAKYSNVSSGGGKMAVSGSLAKRWVEPGKFFRESMLKKGKVMAMPD 434
              +FK Y K G+ FA+YSN  S  GK         +WVE GKFFRE+MLK+G +M MPD
Sbjct: 372 QGQSFKVYTKDGVVFARYSNNVSSNGK------TVNKWVEEGKFFREAMLKEGTLMQMPD 431

Query: 435 IRDKMPKRSFLPRSIVSKLPFSNSKVGVMKEIFHAGENSSMETMMTETLRECERAPSAGE 494
           I+DKMPKR+FLPR+IV  LPFS+S +G +  +F AGENSSM  +++  + ECER  S GE
Sbjct: 432 IKDKMPKRTFLPRNIVKNLPFSSSTIGEIWRVFGAGENSSMAGIISSAVSECERPASHGE 491

Query: 495 TKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNVMIGAVKGINGGKVTQSVSCHQS 554
           TKRCVGS EDMIDFATSVLGR VVVRTTEN  GSK  V+IG V GINGG VT++VSCHQS
Sbjct: 492 TKRCVGSAEDMIDFATSVLGRGVVVRTTENVVGSKKKVVIGKVNGINGGDVTRAVSCHQS 551

Query: 555 LFPYLLYYCHSVPKVRVYEADLLDPSSKATINHGVAICHLDTSAWSPAHGAFIALGSAPG 614
           L+PYLLYYCHSVP+VRVYE DLLDP S   INHGVAICH+DTSAWSP+HGAF+ALGS PG
Sbjct: 552 LYPYLLYYCHSVPRVRVYETDLLDPKSLEKINHGVAICHIDTSAWSPSHGAFLALGSGPG 611

Query: 615 RVEVCHWIFENDMTWAVAD 624
           ++EVCHWIFENDMTW + D
Sbjct: 612 QIEVCHWIFENDMTWNIID 624

BLAST of Spg025633 vs. TAIR 10
Match: AT5G25610.1 (BURP domain-containing protein )

HSP 1 Score: 115.9 bits (289), Expect = 1.1e-25
Identity = 76/219 (34.70%), Postives = 107/219 (48.86%), Query Frame = 0

Query: 408 FFRESMLKKGKVMAMP-DIRDKM-PKRSFLPRSIVSKLPFSNSKVGVMKEIFHAGENSSM 467
           FF E  L +GK M +  +  D    K +FLPR     +PF + K     + F     S  
Sbjct: 176 FFLEKDLVRGKEMNVRFNAEDGYGGKTAFLPRGEAETVPFGSEKFSETLKRFSVEAGSEE 235

Query: 468 ETMMTETLRECERAPSAGETKRCVGSVEDMIDFATSVLGR-NVVVRTTE----NTKGSKG 527
             MM +T+ ECE    +GE K C  S+E M+DF+ S LG+ +V   +TE    N    K 
Sbjct: 236 AEMMKKTIEECEARKVSGEEKYCATSLESMVDFSVSKLGKYHVRAVSTEVAKKNAPMQKY 295

Query: 528 NVMIGAVKGINGGKVTQSVSCHQSLFPYLLYYCHSVPKVRVYEADLLDPSSKATINHGVA 587
            +    VK ++  K   SV CH+  +P+ ++YCH      VY   L   +        VA
Sbjct: 296 KIAAAGVKKLSDDK---SVVCHKQKYPFAVFYCHKAMMTTVYAVPLEGENGMRA--KAVA 355

Query: 588 ICHLDTSAWSPAHGAFIALGSAPGRVEVCHWIFENDMTW 620
           +CH +TSAW+P H AF  L   PG V VCH++ E  + W
Sbjct: 356 VCHKNTSAWNPNHLAFKVLKVKPGTVPVCHFLPETHVVW 389

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038884819.10.0e+0091.65polygalacturonase 1 beta-like protein 3 [Benincasa hispida][more]
XP_023546090.10.0e+0091.17polygalacturonase 1 beta-like protein 3 [Cucurbita pepo subsp. pepo][more]
XP_022961767.10.0e+0091.01polygalacturonase 1 beta-like protein 3 [Cucurbita moschata][more]
XP_008444317.10.0e+0090.53PREDICTED: polygalacturonase 1 beta-like protein 3 [Cucumis melo] >KAA0061089.1 ... [more]
KAG6598207.10.0e+0090.69Polygalacturonase 1 beta-like protein 3, partial [Cucurbita argyrosperma subsp. ... [more]
Match NameE-valueIdentityDescription
P929902.0e-22965.21Polygalacturonase 1 beta-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=PGL3 ... [more]
P929821.2e-22664.92Polygalacturonase 1 beta-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=PGL1 ... [more]
Q401614.4e-21661.29Polygalacturonase-1 non-catalytic subunit beta OS=Solanum lycopersicum OX=4081 G... [more]
O807602.9e-21561.55Polygalacturonase 1 beta-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=PGL2 ... [more]
P932182.8e-21059.97Polygalacturonase non-catalytic subunit AroGP3 OS=Solanum lycopersicum OX=4081 G... [more]
Match NameE-valueIdentityDescription
A0A6J1HB030.0e+0091.01polygalacturonase 1 beta-like protein 3 OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A5A7V5U80.0e+0090.53Polygalacturonase 1 beta-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3B9L40.0e+0090.53polygalacturonase 1 beta-like protein 3 OS=Cucumis melo OX=3656 GN=LOC103487686 ... [more]
A0A6J1K2710.0e+0090.53polygalacturonase 1 beta-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111491... [more]
A0A0A0LK440.0e+0089.73BURP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G348230 PE=4 S... [more]
Match NameE-valueIdentityDescription
AT1G70370.11.4e-23065.21polygalacturonase 2 [more]
AT1G70370.21.4e-23065.21polygalacturonase 2 [more]
AT1G23760.18.8e-22864.92BURP domain-containing protein [more]
AT1G60390.12.0e-21661.55polygalacturonase 1 [more]
AT5G25610.11.1e-2534.70BURP domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004873BURP domainSMARTSM01045BURP_2coord: 406..622
e-value: 2.4E-133
score: 459.0
IPR004873BURP domainPFAMPF03181BURPcoord: 408..620
e-value: 1.4E-65
score: 220.9
IPR004873BURP domainPROSITEPS51277BURPcoord: 408..622
score: 69.706963
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 273..295
NoneNo IPR availablePANTHERPTHR31458POLYGALACTURONASE 1 BETA-LIKE PROTEIN 2coord: 13..623
NoneNo IPR availablePANTHERPTHR31458:SF2POLYGALACTURONASE 1 BETA-LIKE PROTEIN 2coord: 13..623

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg025633.1Spg025633.1mRNA