Spg025588 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg025588
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Histidine--tRNA ligase
Location	scaffold13: 30147208 .. 30148853 (-)
RNA-Seq Expression	Spg025588
Synteny	Spg025588

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTTATTTATTTCAGAAAGTGCAATGGCTCCTTATTTATGAAGCTTCAAGGGATTGGTTGGTTAGTTCTTATACCACTTTGAATATTGTATTCAAGCTTAACTAAATAAAGTGAAATCATGAACTAAATAGTTAGATAAATACAAATAACTGACTTAGGATTGTTTATTGAAATCTTGGAATTTATTTTTTTGTATGTGTGTATAATAAAAAATCTATTCTGACACGGAAGAAGTTCAAATCTCCCACTCTTATTGAATAAATGTCAGTTTTAGTTTATATGTGTAATTCACTTGCATATTTCTAGTTCTAGGTGTTGCTTCAGTTGAATTTATTGATCCAAGAGAGCTTGTGGCAGTTAGGTGTTGTTATTCCTACGATCAAGCATTTGACTGAGAATGGAGCTAAAGTGCTTCTGTCTAGCCATTCGATAAGTGTGCTTTTGGGACTTCTTTGTTTGGCTTTTGTTTTGTTGTGTATGGATGTTGATTGGTGAATCTGGAAAATGGTAGCTATTAAGCATTTGAAGCAAAGCTTCTATCTTCTATTGTTACCTTTTTCAAGCGAATTGGAATTACAGCCCCAGATGTTGGATTCAAGGTTTCCAGTCGGAAGGTATTTTTTTCTGCATCTTGTGTGATCTCCAATAAATTTAGTTCTTGTTTTAGTAGTTATTAAACATTCATTCGTGAATCAAAAGAGTTTCATAGTTATAATTTATGTAAATGCCTTATGTCTTTGGTTTGATGGTAGGTTTTGCAAGAAGTATTAAAATGCTATGGAATACCTGAAAATCTGTTTGGCAAGGTTTGCATCATCTTAGACAAGGTTAGTGATCTACAATTTCAAGTTCCATAAATTTTTATATTTTTGTGCCCTTTCCCTACGCATGTCTGAATGTAATAATTACTACTTTCTTTGTTTCCCTCTGGTTGTTAATCTTAATAACATATAGAGAAATTTCCATTGGATGAGATGACGAAGGAATTGAAGGTCGTGGGTGTATCAGAGGAGGCTATTGAGGAGCTGCTGAAAGTTCTTTCAGTAAAGTTTTTGACAAAGTTAGAAGGTTATTTTCATAATGCTTTAGTCTTTGACAAATTAGTTGGAATGCATGTGATAACTCCAATGAGGACATTGAAACTGGTTGAAGTGTGATTGTTATTCTTGCATAGAAATACTTGGAGGTGGTGGCGAAGCGTTGTCTGATCTAAAGCAGCTATTTTCTCTTGCTGAAAAGTTTGGGTATGTTGATTGGGTCCAATTTGATGCATTCATTGTCTGTGGCCTTGCTTATTAAACTGGTATTGTTTATGAATATTTCCTTTTGAACTCTATCTTCATATAAAATAAAAGTTAATAACTATATTCAATTCTTGCTTATTGTTTTATAGAAATAATTGGGAAATCGATCAAAGTGTAAGTGTTTGTAATTTTGAGGCTTTGGACATGACCTACAAGTGCAGGACTTAATCTTACTTTTTTTTTGCCTAACATTTTTTTTTAGTTTCTAGATCTGGAGTAACAATGGTGAGGATATTTGTCTAGTTAGGAGATCAATACGGTGCACCCTTTTTGTGTTGGCTAAATTTGGAGCAAGTTCAACCATATGTTGAACCTGAATTTAAAGAAGCTATAGGTATG

mRNA sequence

ATGCTTTATTTATTTCAGAAAGTGCAATGGCTCCTTATTTATGAAGCTTCAAGGGATTGGTTGGTGTTGCTTCAGTTGAATTTATTGATCCAAGAGAGCTTGTGGCAGTTAGGTGTTGTTATTCCTACGATCAAGCATTTGACTGAGAATGGAGCTAAAGTGCTTCTGTCTAGCCATTCGATAAGTGTGCTTTTGGGACTTCTTTCCCCAGATGTTGGATTCAAGGTTTCCAGTCGGAAGGTTTTGCAAGAAGTATTAAAATGCTATGGAATACCTGAAAATCTGTTTGGCAAGGTTTGCATCATCTTAGACAAGATGACGAAGGAATTGAAGGTCGTGGGTGTATCAGAGGAGGCTATTGAGGAGCTGCTGAAAGTTCTTTCAGTAAAGTTTTTGACAAAGTTAGAAGAAATACTTGGAGGTGGTGGCGAAGCGTTGTCTGATCTAAAGCAGCTATTTTCTCTTGCTGAAAAGTTTGGGTATGTTGATTGGGTCCAATTTGATGCATTCATTGTCTGTGGCCTTGCTTATTAA

Coding sequence (CDS)

ATGCTTTATTTATTTCAGAAAGTGCAATGGCTCCTTATTTATGAAGCTTCAAGGGATTGGTTGGTGTTGCTTCAGTTGAATTTATTGATCCAAGAGAGCTTGTGGCAGTTAGGTGTTGTTATTCCTACGATCAAGCATTTGACTGAGAATGGAGCTAAAGTGCTTCTGTCTAGCCATTCGATAAGTGTGCTTTTGGGACTTCTTTCCCCAGATGTTGGATTCAAGGTTTCCAGTCGGAAGGTTTTGCAAGAAGTATTAAAATGCTATGGAATACCTGAAAATCTGTTTGGCAAGGTTTGCATCATCTTAGACAAGATGACGAAGGAATTGAAGGTCGTGGGTGTATCAGAGGAGGCTATTGAGGAGCTGCTGAAAGTTCTTTCAGTAAAGTTTTTGACAAAGTTAGAAGAAATACTTGGAGGTGGTGGCGAAGCGTTGTCTGATCTAAAGCAGCTATTTTCTCTTGCTGAAAAGTTTGGGTATGTTGATTGGGTCCAATTTGATGCATTCATTGTCTGTGGCCTTGCTTATTAA

Protein sequence

MLYLFQKVQWLLIYEASRDWLVLLQLNLLIQESLWQLGVVIPTIKHLTENGAKVLLSSHSISVLLGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDKMTKELKVVGVSEEAIEELLKVLSVKFLTKLEEILGGGGEALSDLKQLFSLAEKFGYVDWVQFDAFIVCGLAY

Homology

[Close]

BLAST of Spg025588 vs. NCBI nr
Match: KAG7034545.1 (Histidine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 187.2 bits (474), Expect = 1.2e-43
Identity = 105/160 (65.62%), Postives = 125/160 (78.12%), Query Frame = 0


Query: 29  LIQESLWQLGVVIPTIKHL-TENGAKVLLSSHSISVL--LGLLSPDVGFKVSSRKVLQEV 88
           L+ E L ++     ++ H+  ++GA+  L S  ++    +GL + DVGFKVSSRKVLQEV
Sbjct: 80  LVTEELQKIDGHKRSVTHVRPDSGAEAELLSSIVAFFKRIGLTAADVGFKVSSRKVLQEV 139

Query: 89  LKCYGIPENLFGKVCIILDK--------MTKELKVVGVSEEAIEELLKVLSVKFLTKLEE 148
           LKCYGIPEN+FGKVCII+DK        M KELKVVGVSE+AIEE+L+VLSVK LTKLEE
Sbjct: 140 LKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAIEEMLQVLSVKSLTKLEE 199

Query: 149 ILGGGGEALSDLKQLFSLAEKFGYVDWVQFDAFIVCGLAY 178
           ILGGGGEA+SDLKQLFSLAEKFGY DW+QFDA IV GLAY
Sbjct: 200 ILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAY 239

[Close]

BLAST of Spg025588 vs. NCBI nr
Match: KAG6604395.1 (Histidine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 185.7 bits (470), Expect = 3.6e-43
Identity = 100/134 (74.63%), Postives = 112/134 (83.58%), Query Frame = 0


Query: 52  AKVLLSSHSISVLLGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK------ 111
           A++L S  +    +GL + DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCII+DK      
Sbjct: 224 AELLSSIVAFFKRIGLTAADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPL 283

Query: 112 --MTKELKVVGVSEEAIEELLKVLSVKFLTKLEEILGGGGEALSDLKQLFSLAEKFGYVD 171
             M KELKVVGVSE+AIEE+L+VLSVK LTKLEEILGGGGEA+SDLKQLFSLAEKFGY D
Sbjct: 284 DEMKKELKVVGVSEDAIEEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSD 343

Query: 172 WVQFDAFIVCGLAY 178
           W+QFDA IV GLAY
Sbjct: 344 WIQFDASIVRGLAY 357

[Close]

BLAST of Spg025588 vs. NCBI nr
Match: XP_023544233.1 (histidine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 185.7 bits (470), Expect = 3.6e-43
Identity = 100/134 (74.63%), Postives = 112/134 (83.58%), Query Frame = 0


Query: 52  AKVLLSSHSISVLLGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK------ 111
           A++L S  +    +GL + DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCII+DK      
Sbjct: 224 AELLSSIVAFFKRIGLTAADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPL 283

Query: 112 --MTKELKVVGVSEEAIEELLKVLSVKFLTKLEEILGGGGEALSDLKQLFSLAEKFGYVD 171
             M KELKVVGVSE+AIEE+L+VLSVK LTKLEEILGGGGEA+SDLKQLFSLAEKFGY D
Sbjct: 284 DEMKKELKVVGVSEDAIEEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSD 343

Query: 172 WVQFDAFIVCGLAY 178
           W+QFDA IV GLAY
Sbjct: 344 WIQFDASIVRGLAY 357

[Close]

BLAST of Spg025588 vs. NCBI nr
Match: XP_022978407.1 (histidine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita maxima])

HSP 1 Score: 185.7 bits (470), Expect = 3.6e-43
Identity = 100/134 (74.63%), Postives = 112/134 (83.58%), Query Frame = 0


Query: 52  AKVLLSSHSISVLLGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK------ 111
           A++L S  +    +GL + DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCII+DK      
Sbjct: 224 AELLSSIVAFFKRIGLTAADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPL 283

Query: 112 --MTKELKVVGVSEEAIEELLKVLSVKFLTKLEEILGGGGEALSDLKQLFSLAEKFGYVD 171
             M KELKVVGVSE+AIEE+L+VLSVK LTKLEEILGGGGEA+SDLKQLFSLAEKFGY D
Sbjct: 284 DEMKKELKVVGVSEDAIEEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSD 343

Query: 172 WVQFDAFIVCGLAY 178
           W+QFDA IV GLAY
Sbjct: 344 WIQFDASIVRGLAY 357

[Close]

BLAST of Spg025588 vs. NCBI nr
Match: XP_023544232.1 (histidine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 185.7 bits (470), Expect = 3.6e-43
Identity = 100/134 (74.63%), Postives = 112/134 (83.58%), Query Frame = 0


Query: 52  AKVLLSSHSISVLLGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK------ 111
           A++L S  +    +GL + DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCII+DK      
Sbjct: 224 AELLSSIVAFFKRIGLTAADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPL 283

Query: 112 --MTKELKVVGVSEEAIEELLKVLSVKFLTKLEEILGGGGEALSDLKQLFSLAEKFGYVD 171
             M KELKVVGVSE+AIEE+L+VLSVK LTKLEEILGGGGEA+SDLKQLFSLAEKFGY D
Sbjct: 284 DEMKKELKVVGVSEDAIEEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSD 343

Query: 172 WVQFDAFIVCGLAY 178
           W+QFDA IV GLAY
Sbjct: 344 WIQFDASIVRGLAY 357

[Close]

BLAST of Spg025588 vs. ExPASy Swiss-Prot
Match: O82413 (Histidine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g46100 PE=2 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 4.0e-37
Identity = 79/121 (65.29%), Postives = 99/121 (81.82%), Query Frame = 0


Query: 65  LGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK--------MTKELKVVGVS 124
           +G+ + DVGFKVSSRKVLQE+LK YG+PE+LFG+VCII+DK        + KEL   G+S
Sbjct: 216 IGITASDVGFKVSSRKVLQELLKKYGVPEDLFGRVCIIIDKIEKIPIDEIKKELGFTGIS 275

Query: 125 EEAIEELLKVLSVKFLTKLEEILGGGGEALSDLKQLFSLAEKFGYVDWVQFDAFIVCGLA 178
           E+AIE+LL+VLSVK L  LE+I+GG GEA++DLKQLFSLAEKFGY +W+QFDA +V GLA
Sbjct: 276 EDAIEQLLQVLSVKSLDDLEDIIGGAGEAIADLKQLFSLAEKFGYSEWIQFDASVVRGLA 335

[Close]

BLAST of Spg025588 vs. ExPASy Swiss-Prot
Match: P70076 (Histidine--tRNA ligase, cytoplasmic OS=Takifugu rubripes OX=31033 GN=hars1 PE=3 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 5.8e-04
Identity = 35/125 (28.00%), Postives = 57/125 (45.60%), Query Frame = 0


Query: 71  DVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDKMTK---------ELKVVGVSEEAIE 130
           D   KV+ R++L  +    G+P+N+F  +C  +DK+ K          +   G+SEEA +
Sbjct: 211 DFRIKVNDRRILDGMFAVCGVPDNMFRTICSTVDKLDKLPWEAVKNEMVNEKGLSEEAAD 270

Query: 131 ELLKVLSVKFLTKLEEIL---------GGGGEALSDLKQLFSLAEKFGYVDWVQFDAFIV 178
           ++   + ++    L E L               L+D+K LFS  + F   D V FD  + 
Sbjct: 271 QIGVYVGMQGGMDLAERLLQDQKMCQSTQACAGLTDIKLLFSYLQLFQVTDKVVFDLSLA 330

[Close]

BLAST of Spg025588 vs. ExPASy TrEMBL
Match: A0A6J1IL10 (Histidine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111478405 PE=3 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 1.7e-43
Identity = 100/134 (74.63%), Postives = 112/134 (83.58%), Query Frame = 0


Query: 52  AKVLLSSHSISVLLGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK------ 111
           A++L S  +    +GL + DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCII+DK      
Sbjct: 224 AELLSSIVAFFKRIGLTAADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPL 283

Query: 112 --MTKELKVVGVSEEAIEELLKVLSVKFLTKLEEILGGGGEALSDLKQLFSLAEKFGYVD 171
             M KELKVVGVSE+AIEE+L+VLSVK LTKLEEILGGGGEA+SDLKQLFSLAEKFGY D
Sbjct: 284 DEMKKELKVVGVSEDAIEEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSD 343

Query: 172 WVQFDAFIVCGLAY 178
           W+QFDA IV GLAY
Sbjct: 344 WIQFDASIVRGLAY 357

[Close]

BLAST of Spg025588 vs. ExPASy TrEMBL
Match: A0A6J1IMK6 (Histidine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111478405 PE=3 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 1.7e-43
Identity = 100/134 (74.63%), Postives = 112/134 (83.58%), Query Frame = 0


Query: 52  AKVLLSSHSISVLLGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK------ 111
           A++L S  +    +GL + DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCII+DK      
Sbjct: 224 AELLSSIVAFFKRIGLTAADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPL 283

Query: 112 --MTKELKVVGVSEEAIEELLKVLSVKFLTKLEEILGGGGEALSDLKQLFSLAEKFGYVD 171
             M KELKVVGVSE+AIEE+L+VLSVK LTKLEEILGGGGEA+SDLKQLFSLAEKFGY D
Sbjct: 284 DEMKKELKVVGVSEDAIEEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSD 343

Query: 172 WVQFDAFIVCGLAY 178
           W+QFDA IV GLAY
Sbjct: 344 WIQFDASIVRGLAY 357

[Close]

BLAST of Spg025588 vs. ExPASy TrEMBL
Match: A0A6J1EG18 (Histidine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111433048 PE=3 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 1.7e-43
Identity = 100/134 (74.63%), Postives = 112/134 (83.58%), Query Frame = 0


Query: 52  AKVLLSSHSISVLLGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK------ 111
           A++L S  +    +GL + DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCII+DK      
Sbjct: 224 AELLSSIVAFFKRIGLTAADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPL 283

Query: 112 --MTKELKVVGVSEEAIEELLKVLSVKFLTKLEEILGGGGEALSDLKQLFSLAEKFGYVD 171
             M KELKVVGVSE+AIEE+L+VLSVK LTKLEEILGGGGEA+SDLKQLFSLAEKFGY D
Sbjct: 284 DEMKKELKVVGVSEDAIEEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSD 343

Query: 172 WVQFDAFIVCGLAY 178
           W+QFDA IV GLAY
Sbjct: 344 WIQFDASIVRGLAY 357

[Close]

BLAST of Spg025588 vs. ExPASy TrEMBL
Match: A0A6J1ECF7 (Histidine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111433048 PE=3 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 1.7e-43
Identity = 100/134 (74.63%), Postives = 112/134 (83.58%), Query Frame = 0


Query: 52  AKVLLSSHSISVLLGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK------ 111
           A++L S  +    +GL + DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCII+DK      
Sbjct: 224 AELLSSIVAFFKRIGLTAADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPL 283

Query: 112 --MTKELKVVGVSEEAIEELLKVLSVKFLTKLEEILGGGGEALSDLKQLFSLAEKFGYVD 171
             M KELKVVGVSE+AIEE+L+VLSVK LTKLEEILGGGGEA+SDLKQLFSLAEKFGY D
Sbjct: 284 DEMKKELKVVGVSEDAIEEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSD 343

Query: 172 WVQFDAFIVCGLAY 178
           W+QFDA IV GLAY
Sbjct: 344 WIQFDASIVRGLAY 357

[Close]

BLAST of Spg025588 vs. ExPASy TrEMBL
Match: A0A5D3C5Z0 (Histidine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G00380 PE=3 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 8.6e-43
Identity = 98/134 (73.13%), Postives = 112/134 (83.58%), Query Frame = 0


Query: 52  AKVLLSSHSISVLLGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK------ 111
           A++L S  +    +G+ + DVGFKVSSRKVLQ+VLKCYGIP+N+FGKVCII+DK      
Sbjct: 224 AELLSSIVTFFKRVGITASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPL 283

Query: 112 --MTKELKVVGVSEEAIEELLKVLSVKFLTKLEEILGGGGEALSDLKQLFSLAEKFGYVD 171
             M KELKVVGVSE+AIEELL+VLSVK LTKLEE+LGGGGEALSDLKQLFSLAEKFGY D
Sbjct: 284 DEMKKELKVVGVSEDAIEELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSD 343

Query: 172 WVQFDAFIVCGLAY 178
           W+QFDA IV GLAY
Sbjct: 344 WIQFDASIVRGLAY 357

[Close]

BLAST of Spg025588 vs. TAIR 10
Match: AT3G46100.1 (Histidyl-tRNA synthetase 1 )

HSP 1 Score: 156.0 bits (393), Expect = 2.8e-38
Identity = 79/121 (65.29%), Postives = 99/121 (81.82%), Query Frame = 0


Query: 65  LGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK--------MTKELKVVGVS 124
           +G+ + DVGFKVSSRKVLQE+LK YG+PE+LFG+VCII+DK        + KEL   G+S
Sbjct: 216 IGITASDVGFKVSSRKVLQELLKKYGVPEDLFGRVCIIIDKIEKIPIDEIKKELGFTGIS 275

Query: 125 EEAIEELLKVLSVKFLTKLEEILGGGGEALSDLKQLFSLAEKFGYVDWVQFDAFIVCGLA 178
           E+AIE+LL+VLSVK L  LE+I+GG GEA++DLKQLFSLAEKFGY +W+QFDA +V GLA
Sbjct: 276 EDAIEQLLQVLSVKSLDDLEDIIGGAGEAIADLKQLFSLAEKFGYSEWIQFDASVVRGLA 335

[Close]

BLAST of Spg025588 vs. TAIR 10
Match: AT5G03406.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 66.6 bits (161), Expect = 2.3e-11
Identity = 31/56 (55.36%), Postives = 44/56 (78.57%), Query Frame = 0


Query: 52  AKVLLSSHSISVLLGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDKMT 108
           A+++ S  +    +G+ + DVGFKVSSRKVLQE LK YG+PE+LFG+VC I+DK++
Sbjct: 203 AELISSIMTFFKRIGITASDVGFKVSSRKVLQEFLKKYGVPEDLFGRVCFIIDKVS 258

The following BLAST results are available for this feature:

BLAST of Spg025588 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7034545.1	1.2e-43	65.63	Histidine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosp...	[more]
KAG6604395.1	3.6e-43	74.63	Histidine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosp...	[more]
XP_023544233.1	3.6e-43	74.63	histidine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita pepo s...	[more]
XP_022978407.1	3.6e-43	74.63	histidine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita maxima...	[more]
XP_023544232.1	3.6e-43	74.63	histidine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Cucurbita pepo s...	[more]

BLAST of Spg025588 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O82413	4.0e-37	65.29	Histidine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3...	[more]
P70076	5.8e-04	28.00	Histidine--tRNA ligase, cytoplasmic OS=Takifugu rubripes OX=31033 GN=hars1 PE=3 ...	[more]

BLAST of Spg025588 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IL10	1.7e-43	74.63	Histidine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111478405 PE=3 SV=1	[more]
A0A6J1IMK6	1.7e-43	74.63	Histidine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111478405 PE=3 SV=1	[more]
A0A6J1EG18	1.7e-43	74.63	Histidine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111433048 PE=3 SV=1	[more]
A0A6J1ECF7	1.7e-43	74.63	Histidine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111433048 PE=3 SV=1	[more]
A0A5D3C5Z0	8.6e-43	73.13	Histidine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2...	[more]

BLAST of Spg025588 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G46100.1	2.8e-38	65.29	Histidyl-tRNA synthetase 1	[more]
AT5G03406.1	2.3e-11	55.36	Class II aaRS and biotin synthetases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.930.10	Bira Bifunctional Protein; Domain 2	coord: 31..177 e-value: 1.5E-17 score: 65.6
None	No IPR available	PANTHER	PTHR43707:SF1	HISTIDINE--TRNA LIGASE, MITOCHONDRIAL-RELATED	coord: 32..177
IPR004516	Histidine-tRNA ligase/ATP phosphoribosyltransferase regulatory subunit	PANTHER	PTHR43707	HISTIDYL-TRNA SYNTHETASE	coord: 32..177

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg025588.1	Spg025588.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006427	histidyl-tRNA aminoacylation
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004821	histidine-tRNA ligase activity