Spg025518 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg025518 vs. NCBI nr
Match: XP_022155262.1 (uncharacterized protein LOC111022403 [Momordica charantia])

HSP 1 Score: 87.8 bits (216), Expect = 1.4e-13
Identity = 64/246 (26.02%), Postives = 109/246 (44.31%), Query Frame = 0

Query: 1   MKDNLNGEELAKGAVIMRKIWNYQNGVAINNQRFGAEAFYQSIDFSIKELEVSYLKTQPS 60
           M D  + E+L    +    IWN++N V    +        Q +   +   E SY      
Sbjct: 1   MLDKASDEDLDVLLIGSWVIWNHRNYVIFRGEHSSFSTMIQQLTKFV--TESSYQSETSL 60

Query: 61  DRIGSQASQAPRKNPAP-NCWHLKSDASWNEKLGRGGGGWVVHDLEGSLVCVGMKQITKN 120
             +    +   +  P P + W L +DASW++   RGG GW++   +G +V  G + +   
Sbjct: 61  SMLHKTLNNKLKWEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEAC 120

Query: 121 WPIKMLEAKAMLEGLIQIVDTCKRRSIAIEAQTNALEIINILTRKLEDLSKVKSLIDQIS 180
             +K+LEA A+LEGL  + +    R + IE  T++ E+ ++L RK EDL+K   ++++I 
Sbjct: 121 NNVKLLEASAILEGLRNLTNLGVLRPLHIE--TDSAEVESLLNRKREDLTKTGWVVEEIL 180

Query: 181 FIALDVHDVGFSHYNRVLNTDAHCVERTAVDFPVNVGDLHGSSSSQEKGQVFWAPNLPSW 240
            +      + F+   R  N  AH + + A               S  +  + W    P+W
Sbjct: 181 NLRDSCEILAFAKVGRETNGCAHSLAQRA---------------SVLRESMIWVDCFPNW 227

Query: 241 LFPLFK 246
           L  L K
Sbjct: 241 LSILAK 227

BLAST of Spg025518 vs. NCBI nr
Match: KAG6599977.1 (hypothetical protein SDJN03_05210, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 71.6 bits (174), Expect = 1.1e-08
Identity = 39/102 (38.24%), Postives = 59/102 (57.84%), Query Frame = 0

Query: 82  LKSDASWNEKLGRGGGGWVVHDLEGSLVCVGMKQITKNWPIKMLEAKAMLEG-----LIQ 141
           L S ASW+E+ G+GG  WV+HD  GS +C+  K++ + W +KMLE KAM+EG     LI+
Sbjct: 8   LNSYASWSEEAGKGGVSWVIHDSLGSSICIECKELRRKWLVKMLEGKAMIEGLKTYLLIR 67

Query: 142 IVDTCKRRSIAIEAQTNALEIINILTRKLEDLSKVKSLIDQI 179
              +   R I        LE+  IL     DL ++ +++D+I
Sbjct: 68  ETVSYSHRPIIF------LELARILNNTHMDLIELSNVVDEI 103

BLAST of Spg025518 vs. NCBI nr
Match: XP_022143535.1 (uncharacterized protein LOC111013412 [Momordica charantia])

HSP 1 Score: 68.9 bits (167), Expect = 6.9e-08
Identity = 41/139 (29.50%), Postives = 73/139 (52.52%), Query Frame = 0

Query: 73  KNPAPNCWHLKSDASWNEKLGRGGGGWVVHDLEGSLVCVGMKQITKNWPIKMLEAKAMLE 132
           K P  N W L ++A+W      GG GW++ D +G ++    + I     I  LE  A+ E
Sbjct: 80  KPPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAICE 139

Query: 133 GLIQI-VDTCKRRSIAIEAQTNALEIINILTRKLEDLSKVKSLIDQISFIALDVHDVGFS 192
           GL  I  + C+     I  ++++LE I++L R+ +D +++  L+++I  +  D+  V   
Sbjct: 140 GLRAIRQEHCR----PIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMR 199

Query: 193 HYNRVLNTDAHCVERTAVD 211
           H +R  N  AH + R A++
Sbjct: 200 HISREANKVAHGLARRAME 214

BLAST of Spg025518 vs. NCBI nr
Match: XP_022156777.1 (uncharacterized protein LOC111023608 [Momordica charantia])

HSP 1 Score: 68.6 bits (166), Expect = 9.1e-08
Identity = 46/154 (29.87%), Postives = 77/154 (50.00%), Query Frame = 0

Query: 62  RIGSQASQAPRKNPAPNCWHLKSDASWNEKLGRGGGGWVVHDLEGSLVCVGMKQITKNWP 121
           RIG     A  K P  N W L +DA+W      GG GW++ D +G ++    + I     
Sbjct: 70  RIGDNTG-ARWKPPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERN 129

Query: 122 IKMLEAKAMLEGLIQI-VDTCK----RRSIAIEAQTNALEIINILTRKLEDLSKVKSLID 181
           I  LE  A+ EGL  I  + C+         I  ++++LE I++L R+ +D +++  L++
Sbjct: 130 ITYLEVMAICEGLRAIRQEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLE 189

Query: 182 QISFIALDVHDVGFSHYNRVLNTDAHCVERTAVD 211
           +I  +  D+  V   H +R  N  AH + R A++
Sbjct: 190 EIWQMMEDMKIVSMRHISREANKVAHDLARRAME 222

BLAST of Spg025518 vs. NCBI nr
Match: KNA03763.1 (hypothetical protein SOVF_206000, partial [Spinacia oleracea])

HSP 1 Score: 67.4 bits (163), Expect = 2.0e-07
Identity = 66/251 (26.29%), Postives = 113/251 (45.02%), Query Frame = 0

Query: 6   NGEELAKGAVIMRKIWNYQNGVAINNQRFGAEAFYQSIDFSIKELEVSYLKTQPSDRIGS 65
           N E++   AVI+  IW  +N V +                 ++  + +  K + +  IG 
Sbjct: 45  NREQVELIAVILWNIWFDRNCVRLGGDPKYPATVVAGAQSILEAFQSAKEKLRTAGTIGQ 104

Query: 66  QASQAPRKNPA-----PNCW-HLKSDASWNEKLGRGGGGWVVHDLEGSLVCVGMKQITKN 125
           Q +Q+ ++  A     P  W  + +DA+    LG  G G V+ D EGS   V   Q+  +
Sbjct: 105 QRTQSQQRAQAKWELPPVGWLKVNTDAAIFGDLG-CGLGVVISDYEGSARRVACSQVRHS 164

Query: 126 WPIKMLEAKAMLEGLIQIVDTCKRRSIAIEAQTNALEIINILTRKLEDLSKVKSLIDQIS 185
           W +++ EAKA++ GL Q+ + C  R+I +E   N+L II  L     D S +  ++ ++ 
Sbjct: 165 WSVEITEAKAIVLGL-QVANQCHERNIIVECD-NSL-IIKKLKENKVDGSYLGVILREVK 224

Query: 186 FIALDVHDVGFSHYNRVLNTDAHCVER-TAVDFPVNVGDLHGSSSSQEKGQVFWAPNLPS 245
            ++   H V FSH  R  N  AH +   T V++ V +                WA ++P+
Sbjct: 225 HLSSLFHSVVFSHVFREANEAAHTMAHLTPVNYSVRI----------------WAGSVPN 275

Query: 246 WLFPLFKEDRC 250
            +  +   D C
Sbjct: 285 VVEDVIASDFC 275

BLAST of Spg025518 vs. ExPASy TrEMBL
Match: A0A6J1DNV9 (uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022403 PE=4 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 7.0e-14
Identity = 64/246 (26.02%), Postives = 109/246 (44.31%), Query Frame = 0

Query: 1   MKDNLNGEELAKGAVIMRKIWNYQNGVAINNQRFGAEAFYQSIDFSIKELEVSYLKTQPS 60
           M D  + E+L    +    IWN++N V    +        Q +   +   E SY      
Sbjct: 1   MLDKASDEDLDVLLIGSWVIWNHRNYVIFRGEHSSFSTMIQQLTKFV--TESSYQSETSL 60

Query: 61  DRIGSQASQAPRKNPAP-NCWHLKSDASWNEKLGRGGGGWVVHDLEGSLVCVGMKQITKN 120
             +    +   +  P P + W L +DASW++   RGG GW++   +G +V  G + +   
Sbjct: 61  SMLHKTLNNKLKWEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEAC 120

Query: 121 WPIKMLEAKAMLEGLIQIVDTCKRRSIAIEAQTNALEIINILTRKLEDLSKVKSLIDQIS 180
             +K+LEA A+LEGL  + +    R + IE  T++ E+ ++L RK EDL+K   ++++I 
Sbjct: 121 NNVKLLEASAILEGLRNLTNLGVLRPLHIE--TDSAEVESLLNRKREDLTKTGWVVEEIL 180

Query: 181 FIALDVHDVGFSHYNRVLNTDAHCVERTAVDFPVNVGDLHGSSSSQEKGQVFWAPNLPSW 240
            +      + F+   R  N  AH + + A               S  +  + W    P+W
Sbjct: 181 NLRDSCEILAFAKVGRETNGCAHSLAQRA---------------SVLRESMIWVDCFPNW 227

Query: 241 LFPLFK 246
           L  L K
Sbjct: 241 LSILAK 227

BLAST of Spg025518 vs. ExPASy TrEMBL
Match: A0A6J1CP26 (uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013412 PE=4 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 3.4e-08
Identity = 41/139 (29.50%), Postives = 73/139 (52.52%), Query Frame = 0

Query: 73  KNPAPNCWHLKSDASWNEKLGRGGGGWVVHDLEGSLVCVGMKQITKNWPIKMLEAKAMLE 132
           K P  N W L ++A+W      GG GW++ D +G ++    + I     I  LE  A+ E
Sbjct: 80  KPPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAICE 139

Query: 133 GLIQI-VDTCKRRSIAIEAQTNALEIINILTRKLEDLSKVKSLIDQISFIALDVHDVGFS 192
           GL  I  + C+     I  ++++LE I++L R+ +D +++  L+++I  +  D+  V   
Sbjct: 140 GLRAIRQEHCR----PIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMR 199

Query: 193 HYNRVLNTDAHCVERTAVD 211
           H +R  N  AH + R A++
Sbjct: 200 HISREANKVAHGLARRAME 214

BLAST of Spg025518 vs. ExPASy TrEMBL
Match: A0A6J1DSV1 (uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023608 PE=4 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 4.4e-08
Identity = 46/154 (29.87%), Postives = 77/154 (50.00%), Query Frame = 0

Query: 62  RIGSQASQAPRKNPAPNCWHLKSDASWNEKLGRGGGGWVVHDLEGSLVCVGMKQITKNWP 121
           RIG     A  K P  N W L +DA+W      GG GW++ D +G ++    + I     
Sbjct: 70  RIGDNTG-ARWKPPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERN 129

Query: 122 IKMLEAKAMLEGLIQI-VDTCK----RRSIAIEAQTNALEIINILTRKLEDLSKVKSLID 181
           I  LE  A+ EGL  I  + C+         I  ++++LE I++L R+ +D +++  L++
Sbjct: 130 ITYLEVMAICEGLRAIRQEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLE 189

Query: 182 QISFIALDVHDVGFSHYNRVLNTDAHCVERTAVD 211
           +I  +  D+  V   H +R  N  AH + R A++
Sbjct: 190 EIWQMMEDMKIVSMRHISREANKVAHDLARRAME 222

BLAST of Spg025518 vs. ExPASy TrEMBL
Match: A0A0K9QAP7 (RNase H domain-containing protein (Fragment) OS=Spinacia oleracea OX=3562 GN=SOVF_206000 PE=4 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 9.8e-08
Identity = 66/251 (26.29%), Postives = 113/251 (45.02%), Query Frame = 0

Query: 6   NGEELAKGAVIMRKIWNYQNGVAINNQRFGAEAFYQSIDFSIKELEVSYLKTQPSDRIGS 65
           N E++   AVI+  IW  +N V +                 ++  + +  K + +  IG 
Sbjct: 45  NREQVELIAVILWNIWFDRNCVRLGGDPKYPATVVAGAQSILEAFQSAKEKLRTAGTIGQ 104

Query: 66  QASQAPRKNPA-----PNCW-HLKSDASWNEKLGRGGGGWVVHDLEGSLVCVGMKQITKN 125
           Q +Q+ ++  A     P  W  + +DA+    LG  G G V+ D EGS   V   Q+  +
Sbjct: 105 QRTQSQQRAQAKWELPPVGWLKVNTDAAIFGDLG-CGLGVVISDYEGSARRVACSQVRHS 164

Query: 126 WPIKMLEAKAMLEGLIQIVDTCKRRSIAIEAQTNALEIINILTRKLEDLSKVKSLIDQIS 185
           W +++ EAKA++ GL Q+ + C  R+I +E   N+L II  L     D S +  ++ ++ 
Sbjct: 165 WSVEITEAKAIVLGL-QVANQCHERNIIVECD-NSL-IIKKLKENKVDGSYLGVILREVK 224

Query: 186 FIALDVHDVGFSHYNRVLNTDAHCVER-TAVDFPVNVGDLHGSSSSQEKGQVFWAPNLPS 245
            ++   H V FSH  R  N  AH +   T V++ V +                WA ++P+
Sbjct: 225 HLSSLFHSVVFSHVFREANEAAHTMAHLTPVNYSVRI----------------WAGSVPN 275

Query: 246 WLFPLFKEDRC 250
            +  +   D C
Sbjct: 285 VVEDVIASDFC 275

BLAST of Spg025518 vs. ExPASy TrEMBL
Match: A0A6J1CQG0 (uncharacterized protein LOC111013216 OS=Momordica charantia OX=3673 GN=LOC111013216 PE=4 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 1.8e-06
Identity = 47/177 (26.55%), Postives = 78/177 (44.07%), Query Frame = 0

Query: 5   LNGEELAKGAVIMRKIWNYQNGVAINNQRFGAEAFYQSIDFSIKELEVSYLKTQPSDRIG 64
           L+ EE+A   VI  +IW  +N      +    +   +SI   I            + R  
Sbjct: 26  LSDEEVATSMVIAWQIWESRNRSIFRGETIDEQQLDRSIVLFINSNIDKGTCISQTRRSQ 85

Query: 65  SQASQAPR------------KNPAPNCWHLKSDASWNEKLGRGGGGWVVHDLEGSLVCVG 124
                 PR              P  NCW L +DASW+E+   GG GW++ D  G +V  G
Sbjct: 86  QNDGYLPRGRENLNMLGVRWSAPPTNCWKLNTDASWSEEREVGGIGWILCDCRGEIVLAG 145

Query: 125 MKQITKNWPIKMLEAKAMLEGLIQIVDTCKRRSIAIEAQTNALEIINILTRKLEDLS 170
             +I +   I  LE   ++ GL Q ++   R  I +E  ++++E+I ++ ++  DL+
Sbjct: 146 NCKIREKKEINALELMTIIRGL-QFINMQSRSPIYLE--SDSVEVIRLMKKEDVDLT 199

BLAST of Spg025518 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 46.2 bits (108), Expect = 4.5e-05
Identity = 35/174 (20.11%), Postives = 76/174 (43.68%), Query Frame = 0

Query: 16  IMRKIWNYQNGVAINNQRFGAEAFYQSIDFSIKELEVSYLKTQPSDRIGSQASQAPRKNP 75
           ++ ++W  +N +    + F A+   +  +  ++E  +         +     S   R  P
Sbjct: 363 LLWRLWKNRNELVFRGREFNAQEVLRRAEDDLEEWRIRTEAESCGTKPQVNRSSCGRWRP 422

Query: 76  APNCW-HLKSDASWNEKLGRGGGGWVVHDLEGSLVCVGMKQITKNWPIKMLEAKAMLEGL 135
            P+ W    +DA+WN    R G GWV+ + +G +  +G + + K   +   E +AM   +
Sbjct: 423 PPHQWVKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARALPKLKSVLEAELEAMRWAV 482

Query: 136 IQIVDTCKRRSIAIEAQTNAL-EIIN------ILTRKLEDLSKVKSLIDQISFI 182
           + +    +   +  E+ +  L EI+N       L   ++DL ++ S   ++ F+
Sbjct: 483 LSL-SRFQYNYVIFESDSQVLIEILNNDEIWPSLKPTIQDLQRLLSQFTEVKFV 535