Spg025476 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg025476
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Description2-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic
Locationscaffold13: 29860996 .. 29864067 (+)
RNA-Seq ExpressionSpg025476
SyntenySpg025476
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGCTTCATTCGAGCTTGATTGAGCTGGTGGAGCTTCAAGTTCTTCTTCTTGCTCTTCTTGATTGCTTATTTCCTTATCATATCAGGTCCATTATTATCAATAATATTGAGGAGATTCATTTCTTAATTCTGAAATCTTTACTAATTAGCAAGGGAAAAACACAAGAAAAAAAGAAAAAAAATATTCCCCACCAAGTTCTTAGCAAAAAGAAAAAATCACCAAACATTTGAAGAAAAATAAATTTGAAATTGCTTTGCTGTCGTAATTCTTGACAAAAAAAAAAAAAAAAAAACCATAAAGTTCTCAACAACAAGAAAAAGTCATCTAAATGAAAAGAAAAAAAAAACTCCAAAATTGCTTTCATATCATTGTATAGTTACATGTTCTCCTAAGTAGCTAAAAAAGACAATAAAACCCACAAACACAATAAGAAAAAAAATGAGAGGAAACTCATGTACAACTTCTTTACAGCAAAAATTTTATTCAAACAAAAAGTTTCTTCAATAAATTTCTTATAAGAAACCAACTTCGGTGCAATGTGAATTAAAGAAAAAAGAGGAAATATTCAATTACGTGCTTCAAACAAAAAATAATTGAAGGTCTACATTCCCAAGTTCAGGATTAAAATTGATACGACCGGCTAAGTTTGAGGTTCAATTTGATAAAATTAAAAATTCAGGATTAAAATTGATACAAACTTGTTAAGTTTAGAGTTCAATGTGATGAAATTGAAAGTTTGGGGTTAAAATCGATACCACCTTTCCAACCAAAAAATAAAAATGATTTTTTCCTTTTCATTTTTTATTGTTGGTACAAACATAAACTGGATAAATTGGATTGTGGGCAGATGGCATATGCAGCGAAGTCAAGTCCGCTGGTAATGCTGCAATGGCCGCTTGCGCTTAGGGTGACTCGAGTGGGATCTCCATATCCATAATTCTCGTCAGGTAAACCAGTCATCTGCAATTGCATGGCTTCCTCTCTCCAATTCCAACTTCGTCTTCCTTCTTCAACCACTCCACCTCTTCTGCGTCACCGCCCGATTCAATGCGCTTCCGACCGCCAGGCATTGTTCAACCGTATCGCACCTGTCTATGATAACGTGCGCTTAATTTTATTTTTTTCTTCCTCACTCTCTACTTTCCTATTTGCCTTTCCCCTCTTGAAGTTTTACTTTCTTTCTGCTTCGAATTTAGTTGAATGACTTACTCAGTTTGGGCCAGCATCGGATTTGGAAACGAATGGCCGTCTCCTGGAGCGGGTATGTAATCTTTTCCTTTTCTGATGCGGAATTGTCTTGTCTCTTGTTCAAAATTTTCCTGACCTTTCGAACATTTGGGAACAGTGCAAAGGCAGGGGACTGCGTCTTGGATATATGCTGCGGAAGCGGGGATTTGGCGTTTCTCTTGTCTGAGAAAGTTGGACCCGATGGCAAGGTACCCCGACGGCGACTCCCCATTTGGTTTCTGTACCCATTTTGGTGATCAAGATATCTGATCATCCAATTCTTTGGTGATAATTCACAGGTGACGGGTCTTGATTTCTCTCGGGAGCAGCTAAGTATTGCTTCTTCTCGTCAAAATTCGCTCTCCAAACCCTGCTATAAGAACATTGAGTGAGCATTGGTAAACTTGAACAATAAATATGTTAGCCTGTACTATTTGGAAAATAAATTCCACTCAGTATTTATACGTAAATCAAACTGACGATAGAGTATTGCTGCAGTAAGAATCACAATTTTTTATATTTGTAATTGCTTGTGGTAACCGCTGCTGCCAAATTGAAGGTGGATGGAAGGTGATGCACTTAATTTGCCATTTTCTGACGGCTCTTTCGATGCCATTACAATGGGCTATGGTCTACGAAATGTGGTGGATAAACGGAAAGCTTTGGAGGAGATGCTTAGAGTACTTAAAGAAGGTCTTTTGCTGATTATTTTGTTTTGAGTTTGTGAGTGTTGTACAAATCGGTTCGCTGATGTGCTTTAATTTCTATAACTTCAGTTCTTCTCTCTTTTACTTTACGTCCATGAGCTGCTGCTGATTATGATTTTCAATCCTTTACTTGTGTTTTAAAACTTTCACTGCATATTTTCTGATCTTGGATATGATCTCTCTCTTTATTCCTTCTTGATTGATAACATGGTAGAATTCAGTTACAAAAAATTTGCGTACACATTCTTGACTTAAAAATCAAGTGGTCTTTCCAGGTAGTAAGGTGTCAATTCTCGACTTTAACAAGACTACACAACCGATTACTGCTGCTGTTCAGGTACTTTTATGTAAAGGCCTAGATTTTTGCTGTGTGGTACACACTTCAAATTTAGCGAGTCTCCCAGTTTATATCTCTAAAACCCCTACTTGTTATGCTTGCTTCTTTTGAACTTCCTCTATGCAAGGAATGGATGATCAACAGCATCGTTGTCCCAGTGGCATCTGGTTATGGTCTTGCCGAGGATTATAAATATTTGTCTAAATCCATCAAAGAATTTTTGACAGGCAGGCGCTCTCTGTCTCTCTTTGTCTCTCTCTATTGACAGGGCAACTGCTATTATTAGTTGTTCTAAACAAAAGCCTTCTATTGTTTTTTTTTCCCTTATAATTATGTATTTGGAATCTTCTTTTAGTATCACCCACTGATTAAATTTGTCTTTATACAGGAAAGGAATTGGAAGAACTTGCGTTAGAAGTAGGGTTTTCTACCTCTACACATTATGAAATTAGTGGAGGCCTCATGGGAAATTTGGTGGCTTCTCGTTAAGTGGGATTCATGTTTTGTCTTAGTTCAAATTTCTGGAACTAGAATACTTGTGAGAGTATGGTATTTAGCTTCACGTCTTTCATGCATTAGGTTCTGTGACATTCAACGGAGCTCTGTTTCATGTAAGTTTAAGCAACAGAGAGTATATAATTGCAGTAGGTGACCCATATTTGGTTTGTAATCAATATATAAGGTTCTTCTGGCTCCTTGTCAATTCTATTGAGTTCTCAGTATATGCATACATATACGAGGAATGCAATATAAGTCATAAGCATAACAAAAACATCTAACTGATAATACGTTTTATT

mRNA sequence

ATGGAGCTTCATTCGAGCTTGATTGAGCTGGTGGAGCTTCAAGTTCTTCTTCTTGCTCTTCTTGATTGCTTATTTCCTTATCATATCAGCGAAGTCAAGTCCGCTGGTAAACCAGTCATCTGCAATTGCATGGCTTCCTCTCTCCAATTCCAACTTCGTCTTCCTTCTTCAACCACTCCACCTCTTCTGCGTCACCGCCCGATTCAATGCGCTTCCGACCGCCAGGCATTGTTCAACCGTATCGCACCTGTCTATGATAACTTGAATGACTTACTCAGTTTGGGCCAGCATCGGATTTGGAAACGAATGGCCGTCTCCTGGAGCGGGTATGTAATCTTTTCCTTTTCTGATGCGGAATTGTCTTGTCTCTTGTTCAAAATTTTCCTGACCTTTCGAACATTTGGGAACAGTGCAAAGGCAGGGGACTGCGTCTTGGATATATGCTGCGGAAGCGGGGATTTGGCGTTTCTCTTGTCTGAGAAAGTTGGACCCGATGGCAAGGTGACGGGTCTTGATTTCTCTCGGGAGCAGCTAAGTATTGCTTCTTCTCGTCAAAATTCGCTCTCCAAACCCTGCTATAAGAACATTGAGTGGATGGAAGGTGATGCACTTAATTTGCCATTTTCTGACGGCTCTTTCGATGCCATTACAATGGGCTATGGTCTACGAAATGTGGTGGATAAACGGAAAGCTTTGGAGGAGATGCTTAGAGTACTTAAAGAAGGTAGTAAGGTGTCAATTCTCGACTTTAACAAGACTACACAACCGATTACTGCTGCTGTTCAGGAATGGATGATCAACAGCATCGTTGTCCCAGTGGCATCTGGTTATGGAAAGGAATTGGAAGAACTTGCGTTAGAAGTAGGGTTTTCTACCTCTACACATTATGAAATTAGTGGAGGCCTCATGGGAAATTTGGTGGCTTCTCGTTAA

Coding sequence (CDS)

ATGGAGCTTCATTCGAGCTTGATTGAGCTGGTGGAGCTTCAAGTTCTTCTTCTTGCTCTTCTTGATTGCTTATTTCCTTATCATATCAGCGAAGTCAAGTCCGCTGGTAAACCAGTCATCTGCAATTGCATGGCTTCCTCTCTCCAATTCCAACTTCGTCTTCCTTCTTCAACCACTCCACCTCTTCTGCGTCACCGCCCGATTCAATGCGCTTCCGACCGCCAGGCATTGTTCAACCGTATCGCACCTGTCTATGATAACTTGAATGACTTACTCAGTTTGGGCCAGCATCGGATTTGGAAACGAATGGCCGTCTCCTGGAGCGGGTATGTAATCTTTTCCTTTTCTGATGCGGAATTGTCTTGTCTCTTGTTCAAAATTTTCCTGACCTTTCGAACATTTGGGAACAGTGCAAAGGCAGGGGACTGCGTCTTGGATATATGCTGCGGAAGCGGGGATTTGGCGTTTCTCTTGTCTGAGAAAGTTGGACCCGATGGCAAGGTGACGGGTCTTGATTTCTCTCGGGAGCAGCTAAGTATTGCTTCTTCTCGTCAAAATTCGCTCTCCAAACCCTGCTATAAGAACATTGAGTGGATGGAAGGTGATGCACTTAATTTGCCATTTTCTGACGGCTCTTTCGATGCCATTACAATGGGCTATGGTCTACGAAATGTGGTGGATAAACGGAAAGCTTTGGAGGAGATGCTTAGAGTACTTAAAGAAGGTAGTAAGGTGTCAATTCTCGACTTTAACAAGACTACACAACCGATTACTGCTGCTGTTCAGGAATGGATGATCAACAGCATCGTTGTCCCAGTGGCATCTGGTTATGGAAAGGAATTGGAAGAACTTGCGTTAGAAGTAGGGTTTTCTACCTCTACACATTATGAAATTAGTGGAGGCCTCATGGGAAATTTGGTGGCTTCTCGTTAA

Protein sequence

MELHSSLIELVELQVLLLALLDCLFPYHISEVKSAGKPVICNCMASSLQFQLRLPSSTTPPLLRHRPIQCASDRQALFNRIAPVYDNLNDLLSLGQHRIWKRMAVSWSGYVIFSFSDAELSCLLFKIFLTFRTFGNSAKAGDCVLDICCGSGDLAFLLSEKVGPDGKVTGLDFSREQLSIASSRQNSLSKPCYKNIEWMEGDALNLPFSDGSFDAITMGYGLRNVVDKRKALEEMLRVLKEGSKVSILDFNKTTQPITAAVQEWMINSIVVPVASGYGKELEELALEVGFSTSTHYEISGGLMGNLVASR
Homology
BLAST of Spg025476 vs. NCBI nr
Match: XP_022951506.1 (2-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 435.3 bits (1118), Expect = 4.5e-118
Identity = 226/291 (77.66%), Postives = 234/291 (80.41%), Query Frame = 0

Query: 38  PVICNCMASSLQFQLRLPSSTTPPLLRHRPIQCASDRQALFNRIAPVYDNLNDLLSLGQH 97
           P ICN MASSL  QLRLP  T PP LRHRPIQCA+DRQALFNRIAPVYDNLNDLLSLGQH
Sbjct: 15  PAICNRMASSLPLQLRLPPPTNPPFLRHRPIQCAADRQALFNRIAPVYDNLNDLLSLGQH 74

Query: 98  RIWKRMAVSWSGYVIFSFSDAELSCLLFKIFLTFRTFGNSAKAGDCVLDICCGSGDLAFL 157
           RIWKRMAVSWSG                            AKAGDCVLDICCGSGDLAFL
Sbjct: 75  RIWKRMAVSWSG----------------------------AKAGDCVLDICCGSGDLAFL 134

Query: 158 LSEKVGPDGKVTGLDFSREQLSIASSRQNSLSKPCYKNIEWMEGDALNLPFSDGSFDAIT 217
           LSE+VG DGKVTGLDFS+EQLSIASSRQNSLSKPCY NIEW+EGDALNLPFSDG+FDAIT
Sbjct: 135 LSERVGYDGKVTGLDFSQEQLSIASSRQNSLSKPCYNNIEWVEGDALNLPFSDGTFDAIT 194

Query: 218 MGYGLRNVVDKRKALEEMLRVLKEGSKVSILDFNKTTQPITAAVQEWMINSIVVPVASGY 277
           MGYGLRNVVDKRKALEEMLRVLK+GSKVSILDFNKTTQPITAA+QEWMIN+IVVPVASGY
Sbjct: 195 MGYGLRNVVDKRKALEEMLRVLKQGSKVSILDFNKTTQPITAAIQEWMINNIVVPVASGY 254

Query: 278 ------------------GKELEELALEVGFSTSTHYEISGGLMGNLVASR 311
                             GKELEELALEVGFSTS HYEISGGLMGNLVASR
Sbjct: 255 GLAEDYKYLSKSINEFSTGKELEELALEVGFSTSRHYEISGGLMGNLVASR 277

BLAST of Spg025476 vs. NCBI nr
Match: XP_023002763.1 (2-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 433.3 bits (1113), Expect = 1.7e-117
Identity = 224/291 (76.98%), Postives = 234/291 (80.41%), Query Frame = 0

Query: 38  PVICNCMASSLQFQLRLPSSTTPPLLRHRPIQCASDRQALFNRIAPVYDNLNDLLSLGQH 97
           P ICN MASSL  QLRLP  T PP LRHRPIQCA+DRQALFNRIAPVYDNLNDLLSLGQH
Sbjct: 15  PAICNRMASSLPLQLRLPLPTNPPFLRHRPIQCAADRQALFNRIAPVYDNLNDLLSLGQH 74

Query: 98  RIWKRMAVSWSGYVIFSFSDAELSCLLFKIFLTFRTFGNSAKAGDCVLDICCGSGDLAFL 157
           RIWKRMAVSWSG                            AKAGDCVLDICCGSGDLAFL
Sbjct: 75  RIWKRMAVSWSG----------------------------AKAGDCVLDICCGSGDLAFL 134

Query: 158 LSEKVGPDGKVTGLDFSREQLSIASSRQNSLSKPCYKNIEWMEGDALNLPFSDGSFDAIT 217
           +SE+VG DGKVTGLDFS+EQLSIASSRQNSLSKPCY NIEW+EGDALNLPFSDG+FDAIT
Sbjct: 135 VSERVGYDGKVTGLDFSQEQLSIASSRQNSLSKPCYNNIEWVEGDALNLPFSDGTFDAIT 194

Query: 218 MGYGLRNVVDKRKALEEMLRVLKEGSKVSILDFNKTTQPITAAVQEWMINSIVVPVASGY 277
           MGYGLRNVVDKRKALEEMLRVLK+GSK+SILDFNKTTQPITAA+QEWMIN+IVVPVASGY
Sbjct: 195 MGYGLRNVVDKRKALEEMLRVLKQGSKISILDFNKTTQPITAAIQEWMINNIVVPVASGY 254

Query: 278 ------------------GKELEELALEVGFSTSTHYEISGGLMGNLVASR 311
                             GKELEELALEVGFSTS HYEISGGLMGNLVASR
Sbjct: 255 GLAEDYKYLSKSINEFSTGKELEELALEVGFSTSRHYEISGGLMGNLVASR 277

BLAST of Spg025476 vs. NCBI nr
Match: XP_022951505.1 (2-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 429.5 bits (1103), Expect = 2.5e-116
Identity = 226/295 (76.61%), Postives = 234/295 (79.32%), Query Frame = 0

Query: 38  PVICNCMASSLQFQLRLPSSTTPPLLRHRPIQCASDRQALFNRIAPVYDNLNDLLSLGQH 97
           P ICN MASSL  QLRLP  T PP LRHRPIQCA+DRQALFNRIAPVYDNLNDLLSLGQH
Sbjct: 15  PAICNRMASSLPLQLRLPPPTNPPFLRHRPIQCAADRQALFNRIAPVYDNLNDLLSLGQH 74

Query: 98  RIWKRMAVSWSGYVIFSFSDAELSCLLFKIFLTFRTFGNSAKAGDCVLDICCGSGDLAFL 157
           RIWKRMAVSWSG                            AKAGDCVLDICCGSGDLAFL
Sbjct: 75  RIWKRMAVSWSG----------------------------AKAGDCVLDICCGSGDLAFL 134

Query: 158 LSEKVGPDGKVTGLDFSREQLSIASSRQNSLSKPCYKNIE----WMEGDALNLPFSDGSF 217
           LSE+VG DGKVTGLDFS+EQLSIASSRQNSLSKPCY NIE    W+EGDALNLPFSDG+F
Sbjct: 135 LSERVGYDGKVTGLDFSQEQLSIASSRQNSLSKPCYNNIEVLLQWVEGDALNLPFSDGTF 194

Query: 218 DAITMGYGLRNVVDKRKALEEMLRVLKEGSKVSILDFNKTTQPITAAVQEWMINSIVVPV 277
           DAITMGYGLRNVVDKRKALEEMLRVLK+GSKVSILDFNKTTQPITAA+QEWMIN+IVVPV
Sbjct: 195 DAITMGYGLRNVVDKRKALEEMLRVLKQGSKVSILDFNKTTQPITAAIQEWMINNIVVPV 254

Query: 278 ASGY------------------GKELEELALEVGFSTSTHYEISGGLMGNLVASR 311
           ASGY                  GKELEELALEVGFSTS HYEISGGLMGNLVASR
Sbjct: 255 ASGYGLAEDYKYLSKSINEFSTGKELEELALEVGFSTSRHYEISGGLMGNLVASR 281

BLAST of Spg025476 vs. NCBI nr
Match: XP_038885206.1 (2-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic [Benincasa hispida])

HSP 1 Score: 420.6 bits (1080), Expect = 1.2e-113
Identity = 221/285 (77.54%), Postives = 228/285 (80.00%), Query Frame = 0

Query: 44  MASSLQFQLRLPSSTTPPLLRHRPIQCASDRQALFNRIAPVYDNLNDLLSLGQHRIWKRM 103
           MASSLQ QLRLP STTPPLLRHRPIQCASDRQALFNRIAPVYD LNDLLSLGQHRIWKRM
Sbjct: 1   MASSLQLQLRLPPSTTPPLLRHRPIQCASDRQALFNRIAPVYDTLNDLLSLGQHRIWKRM 60

Query: 104 AVSWSGYVIFSFSDAELSCLLFKIFLTFRTFGNSAKAGDCVLDICCGSGDLAFLLSEKVG 163
           AVSWSG                            AKAGDCVLD+CCGSGDLAFLLS+K+G
Sbjct: 61  AVSWSG----------------------------AKAGDCVLDLCCGSGDLAFLLSQKIG 120

Query: 164 PDGKVTGLDFSREQLSIASSRQNSLSKPCYKNIEWMEGDALNLPFSDGSFDAITMGYGLR 223
             GKVTGLDFSREQLSIASSRQNSLSKP Y NIEW+EGDALNLPF D SFDAITMGYGLR
Sbjct: 121 SVGKVTGLDFSREQLSIASSRQNSLSKPFYNNIEWVEGDALNLPFPDCSFDAITMGYGLR 180

Query: 224 NVVDKRKALEEMLRVLKEGSKVSILDFNKTTQPITAAVQEWMINSIVVPVASGY------ 283
           NV+DKRKALEEMLRVLKEGSKVSILDFNKTTQP TAA+QEWMIN+IVVPVASGY      
Sbjct: 181 NVMDKRKALEEMLRVLKEGSKVSILDFNKTTQPTTAAIQEWMINNIVVPVASGYGLAEDY 240

Query: 284 ------------GKELEELALEVGFSTSTHYEISGGLMGNLVASR 311
                       GKELEELALEVGFSTS HYEISGGLMGNLVASR
Sbjct: 241 KYLSKSINEFSTGKELEELALEVGFSTSIHYEISGGLMGNLVASR 257

BLAST of Spg025476 vs. NCBI nr
Match: XP_023545823.1 (2-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 416.4 bits (1069), Expect = 2.2e-112
Identity = 216/285 (75.79%), Postives = 229/285 (80.35%), Query Frame = 0

Query: 44  MASSLQFQLRLPSSTTPPLLRHRPIQCASDRQALFNRIAPVYDNLNDLLSLGQHRIWKRM 103
           MASSLQ Q RLP STTP LLRHRPIQCA+DRQALF+RIAPVYDNLNDLLSLGQHRIWKRM
Sbjct: 1   MASSLQLQFRLPPSTTPALLRHRPIQCATDRQALFDRIAPVYDNLNDLLSLGQHRIWKRM 60

Query: 104 AVSWSGYVIFSFSDAELSCLLFKIFLTFRTFGNSAKAGDCVLDICCGSGDLAFLLSEKVG 163
           AVSWSG                            AKAGDCVLDICCGSGDLAFLLS+KVG
Sbjct: 61  AVSWSG----------------------------AKAGDCVLDICCGSGDLAFLLSQKVG 120

Query: 164 PDGKVTGLDFSREQLSIASSRQNSLSKPCYKNIEWMEGDALNLPFSDGSFDAITMGYGLR 223
            +GKVTGLDFSR+QLSIASSRQ SLS+PCYKNIEW+EGDALNLPFSDGS DAITMGYGLR
Sbjct: 121 SNGKVTGLDFSRQQLSIASSRQTSLSRPCYKNIEWVEGDALNLPFSDGSVDAITMGYGLR 180

Query: 224 NVVDKRKALEEMLRVLKEGSKVSILDFNKTTQPITAAVQEWMINSIVVPVASGY------ 283
           NV+DKRKALEEMLRVLKEGSK+SILDFNK TQP TAA+QEWMIN+IVVP+ASGY      
Sbjct: 181 NVLDKRKALEEMLRVLKEGSKLSILDFNKATQPTTAAIQEWMINNIVVPMASGYGLAEDY 240

Query: 284 ------------GKELEELALEVGFSTSTHYEISGGLMGNLVASR 311
                       GKELE+LALEVGFSTS HYEISGGLMGNLVASR
Sbjct: 241 KYLSKSISEFSTGKELEKLALEVGFSTSIHYEISGGLMGNLVASR 257

BLAST of Spg025476 vs. ExPASy Swiss-Prot
Match: Q3ED65 (2-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MENG PE=1 SV=2)

HSP 1 Score: 302.0 bits (772), Expect = 7.9e-81
Identity = 154/265 (58.11%), Postives = 187/265 (70.57%), Query Frame = 0

Query: 64  RHRPIQCASDRQALFNRIAPVYDNLNDLLSLGQHRIWKRMAVSWSGYVIFSFSDAELSCL 123
           R   ++C+++R+ LFNRIAPVYDNLNDLLSLGQHRIWK MAVSWSG              
Sbjct: 25  RRTVVKCSNERRILFNRIAPVYDNLNDLLSLGQHRIWKNMAVSWSG-------------- 84

Query: 124 LFKIFLTFRTFGNSAKAGDCVLDICCGSGDLAFLLSEKVGPDGKVTGLDFSREQLSIASS 183
                         AK GD VLD+CCGSGDLAFLLSEKVG  GKV GLDFS EQL++A++
Sbjct: 85  --------------AKKGDYVLDLCCGSGDLAFLLSEKVGSTGKVMGLDFSSEQLAVAAT 144

Query: 184 RQNSLSKPCYKNIEWMEGDALNLPFSDGSFDAITMGYGLRNVVDKRKALEEMLRVLKEGS 243
           RQ+  ++ CYK IEW+EGDA++LPF D  FDA+TMGYGLRNVVD+ +A++EM RVLK GS
Sbjct: 145 RQSLKARSCYKCIEWIEGDAIDLPFDDCEFDAVTMGYGLRNVVDRLRAMKEMYRVLKPGS 204

Query: 244 KVSILDFNKTTQPITAAVQEWMINSIVVPVASGY------------------GKELEELA 303
           +VSILDFNK+ Q +T  +Q WMI+++VVPVA+ Y                  G+ELE LA
Sbjct: 205 RVSILDFNKSNQSVTTFMQGWMIDNVVVPVATVYDLAKEYEYLKYSINGYLTGEELETLA 261

Query: 304 LEVGFSTSTHYEISGGLMGNLVASR 311
           LE GFS++ HYEISGG MGNLVA R
Sbjct: 265 LEAGFSSACHYEISGGFMGNLVAMR 261

BLAST of Spg025476 vs. ExPASy Swiss-Prot
Match: B2IUM7 (2-phytyl-1,4-naphtoquinone methyltransferase OS=Nostoc punctiforme (strain ATCC 29133 / PCC 73102) OX=63737 GN=menG PE=3 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 3.1e-53
Identity = 119/255 (46.67%), Postives = 148/255 (58.04%), Query Frame = 0

Query: 75  QALFNRIAPVYDNLNDLLSLGQHRIWKRMAVSWSGYVIFSFSDAELSCLLFKIFLTFRTF 134
           Q++FNRIAPVYD LND LSLGQHRIWK MAV WS                          
Sbjct: 6   QSIFNRIAPVYDQLNDWLSLGQHRIWKEMAVKWS-------------------------- 65

Query: 135 GNSAKAGDCVLDICCGSGDLAFLLSEKVGPDGKVTGLDFSREQLSIASSR-QNSLSKPCY 194
             +AK+G+  LD+CCGSGDLA  L+ +VG  G V G+DFS   L  A  R Q    +P  
Sbjct: 66  --AAKSGNTALDLCCGSGDLALRLARRVGATGYVYGVDFSCNLLETAKERSQKQYPQPA- 125

Query: 195 KNIEWMEGDALNLPFSDGSFDAITMGYGLRNVVDKRKALEEMLRVLKEGSKVSILDFNKT 254
             I W+E D LNLPF D  FDA TMGYGLRNV D  ++L+E+ RVLK G+K +ILDF++ 
Sbjct: 126 --IAWVEADVLNLPFDDNQFDAATMGYGLRNVKDIPRSLQELHRVLKPGAKAAILDFHRP 185

Query: 255 TQPITAAVQEWMINSIVVPVAS------------------GYGKELEELALEVGFSTSTH 311
           + P   A Q+  +NS VVPVA+                    GKE  ELA +VGF+ +TH
Sbjct: 186 SNPQLRAFQQLYLNSFVVPVANYLGLKEEYAYISPSLDRFPIGKEQIELARQVGFAVATH 229

BLAST of Spg025476 vs. ExPASy Swiss-Prot
Match: Q8YLP4 (2-phytyl-1,4-naphtoquinone methyltransferase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=menG PE=3 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 9.0e-53
Identity = 113/255 (44.31%), Postives = 150/255 (58.82%), Query Frame = 0

Query: 75  QALFNRIAPVYDNLNDLLSLGQHRIWKRMAVSWSGYVIFSFSDAELSCLLFKIFLTFRTF 134
           +A+F+RIAPVYD LND LSLGQHRIWK MA+ W+G                         
Sbjct: 6   RAIFDRIAPVYDQLNDWLSLGQHRIWKEMAIKWTG------------------------- 65

Query: 135 GNSAKAGDCVLDICCGSGDLAFLLSEKVGPDGKVTGLDFSREQLSIASSR-QNSLSKPCY 194
              AK GD  LD+CCGSGDLA  L+ +VG  G+V+G+DFS   L  A  R Q+   +P  
Sbjct: 66  ---AKPGDTCLDLCCGSGDLALRLARRVGSTGQVSGVDFSANLLETAKQRAQSQYPQP-- 125

Query: 195 KNIEWMEGDALNLPFSDGSFDAITMGYGLRNVVDKRKALEEMLRVLKEGSKVSILDFNKT 254
            NI W+E + L+LPF D  FDA TMGYGLRNV D  ++L+E+ RVLK  +K +ILDF++ 
Sbjct: 126 -NISWVEANVLDLPFKDNQFDAATMGYGLRNVTDIPRSLQELHRVLKPNAKAAILDFHRP 185

Query: 255 TQPITAAVQEWMINSIVVPVAS------------------GYGKELEELALEVGFSTSTH 311
                   Q+W ++SIVVP+A                     GKE  E+AL+VGF+++TH
Sbjct: 186 NNQQFRTFQQWYLDSIVVPLADRLGVKEEYAYISPSLDRFPIGKEQVEIALKVGFTSATH 229

BLAST of Spg025476 vs. ExPASy Swiss-Prot
Match: Q3MD91 (2-phytyl-1,4-naphtoquinone methyltransferase OS=Trichormus variabilis (strain ATCC 29413 / PCC 7937) OX=240292 GN=menG PE=3 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 1.0e-51
Identity = 112/255 (43.92%), Postives = 148/255 (58.04%), Query Frame = 0

Query: 75  QALFNRIAPVYDNLNDLLSLGQHRIWKRMAVSWSGYVIFSFSDAELSCLLFKIFLTFRTF 134
           +A+F+RIAPVYD LND LSLGQHRIWK MA+ W+G                         
Sbjct: 6   RAIFDRIAPVYDQLNDWLSLGQHRIWKEMAIKWTG------------------------- 65

Query: 135 GNSAKAGDCVLDICCGSGDLAFLLSEKVGPDGKVTGLDFSREQLSIASSR-QNSLSKPCY 194
              AK GD  LD+CCGSGDLA  L+ +VG  G+V G+DFS   L  A  R Q    +P  
Sbjct: 66  ---AKPGDTCLDLCCGSGDLALRLARRVGSTGQVYGVDFSANLLETAKQRAQAQYPQP-- 125

Query: 195 KNIEWMEGDALNLPFSDGSFDAITMGYGLRNVVDKRKALEEMLRVLKEGSKVSILDFNKT 254
            +I W+E + L+LPF D  FDA TMGYGLRNV D  ++L+E+ RVLK  +K +ILDF++ 
Sbjct: 126 -HISWVEANVLDLPFEDNQFDAATMGYGLRNVTDIPRSLQELRRVLKPNAKAAILDFHRP 185

Query: 255 TQPITAAVQEWMINSIVVPVAS------------------GYGKELEELALEVGFSTSTH 311
                   Q+W ++SIVVP+A                     GKE  E+AL+VGF+++TH
Sbjct: 186 NNQQFRTFQQWYLDSIVVPLADRLGVKEEYAYISPSLDRFPIGKEQVEIALQVGFTSATH 229

BLAST of Spg025476 vs. ExPASy Swiss-Prot
Match: P72818 (2-phytyl-1,4-naphtoquinone methyltransferase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=menG PE=3 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 1.6e-49
Identity = 111/254 (43.70%), Postives = 143/254 (56.30%), Query Frame = 0

Query: 75  QALFNRIAPVYDNLNDLLSLGQHRIWKRMAVSWSGYVIFSFSDAELSCLLFKIFLTFRTF 134
           Q +F RIAP YD+LN  LS GQH IWK MAV WSG                         
Sbjct: 15  QQIFARIAPQYDDLNTFLSFGQHHIWKAMAVKWSG------------------------- 74

Query: 135 GNSAKAGDCVLDICCGSGDLAFLLSEKVGPDGKVTGLDFSREQLSIASSRQNSLSKPCYK 194
                 GD +LD+CCGSGDLAF  ++ VG  GKV GLDF  E L+IA+ +    SK  + 
Sbjct: 75  ---VSPGDRLLDVCCGSGDLAFQGAKVVGTRGKVVGLDFCAELLAIAAGKHK--SKYAHL 134

Query: 195 NIEWMEGDALNLPFSDGSFDAITMGYGLRNVVDKRKALEEMLRVLKEGSKVSILDFNKTT 254
            ++W++GDAL LPFSD  FD  TMGYGLRNV +  +AL E+ RVLK G KV+ILDF++  
Sbjct: 135 PMQWLQGDALALPFSDNEFDGATMGYGLRNVGNIPQALTELQRVLKPGKKVAILDFHQPG 194

Query: 255 QPITAAVQEWMINSIVVPVASGY------------------GKELEELALEVGFSTSTHY 311
             + A  Q W + ++VVP+A  +                  G +  + ALEVGF+ + HY
Sbjct: 195 NALAANFQRWYLANVVVPMAKQWRLTEEYAYLQPSLDRFPTGPKQVQFALEVGFAKAVHY 238

BLAST of Spg025476 vs. ExPASy TrEMBL
Match: A0A6J1GHS2 (2-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111454302 PE=3 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 2.2e-118
Identity = 226/291 (77.66%), Postives = 234/291 (80.41%), Query Frame = 0

Query: 38  PVICNCMASSLQFQLRLPSSTTPPLLRHRPIQCASDRQALFNRIAPVYDNLNDLLSLGQH 97
           P ICN MASSL  QLRLP  T PP LRHRPIQCA+DRQALFNRIAPVYDNLNDLLSLGQH
Sbjct: 15  PAICNRMASSLPLQLRLPPPTNPPFLRHRPIQCAADRQALFNRIAPVYDNLNDLLSLGQH 74

Query: 98  RIWKRMAVSWSGYVIFSFSDAELSCLLFKIFLTFRTFGNSAKAGDCVLDICCGSGDLAFL 157
           RIWKRMAVSWSG                            AKAGDCVLDICCGSGDLAFL
Sbjct: 75  RIWKRMAVSWSG----------------------------AKAGDCVLDICCGSGDLAFL 134

Query: 158 LSEKVGPDGKVTGLDFSREQLSIASSRQNSLSKPCYKNIEWMEGDALNLPFSDGSFDAIT 217
           LSE+VG DGKVTGLDFS+EQLSIASSRQNSLSKPCY NIEW+EGDALNLPFSDG+FDAIT
Sbjct: 135 LSERVGYDGKVTGLDFSQEQLSIASSRQNSLSKPCYNNIEWVEGDALNLPFSDGTFDAIT 194

Query: 218 MGYGLRNVVDKRKALEEMLRVLKEGSKVSILDFNKTTQPITAAVQEWMINSIVVPVASGY 277
           MGYGLRNVVDKRKALEEMLRVLK+GSKVSILDFNKTTQPITAA+QEWMIN+IVVPVASGY
Sbjct: 195 MGYGLRNVVDKRKALEEMLRVLKQGSKVSILDFNKTTQPITAAIQEWMINNIVVPVASGY 254

Query: 278 ------------------GKELEELALEVGFSTSTHYEISGGLMGNLVASR 311
                             GKELEELALEVGFSTS HYEISGGLMGNLVASR
Sbjct: 255 GLAEDYKYLSKSINEFSTGKELEELALEVGFSTSRHYEISGGLMGNLVASR 277

BLAST of Spg025476 vs. ExPASy TrEMBL
Match: A0A6J1KUI9 (2-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111496525 PE=3 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 8.3e-118
Identity = 224/291 (76.98%), Postives = 234/291 (80.41%), Query Frame = 0

Query: 38  PVICNCMASSLQFQLRLPSSTTPPLLRHRPIQCASDRQALFNRIAPVYDNLNDLLSLGQH 97
           P ICN MASSL  QLRLP  T PP LRHRPIQCA+DRQALFNRIAPVYDNLNDLLSLGQH
Sbjct: 15  PAICNRMASSLPLQLRLPLPTNPPFLRHRPIQCAADRQALFNRIAPVYDNLNDLLSLGQH 74

Query: 98  RIWKRMAVSWSGYVIFSFSDAELSCLLFKIFLTFRTFGNSAKAGDCVLDICCGSGDLAFL 157
           RIWKRMAVSWSG                            AKAGDCVLDICCGSGDLAFL
Sbjct: 75  RIWKRMAVSWSG----------------------------AKAGDCVLDICCGSGDLAFL 134

Query: 158 LSEKVGPDGKVTGLDFSREQLSIASSRQNSLSKPCYKNIEWMEGDALNLPFSDGSFDAIT 217
           +SE+VG DGKVTGLDFS+EQLSIASSRQNSLSKPCY NIEW+EGDALNLPFSDG+FDAIT
Sbjct: 135 VSERVGYDGKVTGLDFSQEQLSIASSRQNSLSKPCYNNIEWVEGDALNLPFSDGTFDAIT 194

Query: 218 MGYGLRNVVDKRKALEEMLRVLKEGSKVSILDFNKTTQPITAAVQEWMINSIVVPVASGY 277
           MGYGLRNVVDKRKALEEMLRVLK+GSK+SILDFNKTTQPITAA+QEWMIN+IVVPVASGY
Sbjct: 195 MGYGLRNVVDKRKALEEMLRVLKQGSKISILDFNKTTQPITAAIQEWMINNIVVPVASGY 254

Query: 278 ------------------GKELEELALEVGFSTSTHYEISGGLMGNLVASR 311
                             GKELEELALEVGFSTS HYEISGGLMGNLVASR
Sbjct: 255 GLAEDYKYLSKSINEFSTGKELEELALEVGFSTSRHYEISGGLMGNLVASR 277

BLAST of Spg025476 vs. ExPASy TrEMBL
Match: A0A6J1GIZ3 (2-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111454302 PE=3 SV=1)

HSP 1 Score: 429.5 bits (1103), Expect = 1.2e-116
Identity = 226/295 (76.61%), Postives = 234/295 (79.32%), Query Frame = 0

Query: 38  PVICNCMASSLQFQLRLPSSTTPPLLRHRPIQCASDRQALFNRIAPVYDNLNDLLSLGQH 97
           P ICN MASSL  QLRLP  T PP LRHRPIQCA+DRQALFNRIAPVYDNLNDLLSLGQH
Sbjct: 15  PAICNRMASSLPLQLRLPPPTNPPFLRHRPIQCAADRQALFNRIAPVYDNLNDLLSLGQH 74

Query: 98  RIWKRMAVSWSGYVIFSFSDAELSCLLFKIFLTFRTFGNSAKAGDCVLDICCGSGDLAFL 157
           RIWKRMAVSWSG                            AKAGDCVLDICCGSGDLAFL
Sbjct: 75  RIWKRMAVSWSG----------------------------AKAGDCVLDICCGSGDLAFL 134

Query: 158 LSEKVGPDGKVTGLDFSREQLSIASSRQNSLSKPCYKNIE----WMEGDALNLPFSDGSF 217
           LSE+VG DGKVTGLDFS+EQLSIASSRQNSLSKPCY NIE    W+EGDALNLPFSDG+F
Sbjct: 135 LSERVGYDGKVTGLDFSQEQLSIASSRQNSLSKPCYNNIEVLLQWVEGDALNLPFSDGTF 194

Query: 218 DAITMGYGLRNVVDKRKALEEMLRVLKEGSKVSILDFNKTTQPITAAVQEWMINSIVVPV 277
           DAITMGYGLRNVVDKRKALEEMLRVLK+GSKVSILDFNKTTQPITAA+QEWMIN+IVVPV
Sbjct: 195 DAITMGYGLRNVVDKRKALEEMLRVLKQGSKVSILDFNKTTQPITAAIQEWMINNIVVPV 254

Query: 278 ASGY------------------GKELEELALEVGFSTSTHYEISGGLMGNLVASR 311
           ASGY                  GKELEELALEVGFSTS HYEISGGLMGNLVASR
Sbjct: 255 ASGYGLAEDYKYLSKSINEFSTGKELEELALEVGFSTSRHYEISGGLMGNLVASR 281

BLAST of Spg025476 vs. ExPASy TrEMBL
Match: A0A6J1BRH7 (2-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111004744 PE=3 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 2.0e-111
Identity = 218/285 (76.49%), Postives = 225/285 (78.95%), Query Frame = 0

Query: 44  MASSLQFQLRLPSSTTPPLLRHRPIQCASDRQALFNRIAPVYDNLNDLLSLGQHRIWKRM 103
           MASSLQ QLRLPSSTTP L  HRPI+CA+DRQALFNRIAPVYDNLNDLLSLGQHRIWKRM
Sbjct: 1   MASSLQLQLRLPSSTTPTLQTHRPIRCAADRQALFNRIAPVYDNLNDLLSLGQHRIWKRM 60

Query: 104 AVSWSGYVIFSFSDAELSCLLFKIFLTFRTFGNSAKAGDCVLDICCGSGDLAFLLSEKVG 163
           AVSWSG                            AK GD VLDICCGSGDLAFLLSE++G
Sbjct: 61  AVSWSG----------------------------AKPGDRVLDICCGSGDLAFLLSERLG 120

Query: 164 PDGKVTGLDFSREQLSIASSRQNSLSKPCYKNIEWMEGDALNLPFSDGSFDAITMGYGLR 223
            DGKVTGLDFSREQL IASSRQ SLSK CYKNIEW+EGDALNLPFSDG FDAITMGYGLR
Sbjct: 121 SDGKVTGLDFSREQLIIASSRQRSLSKACYKNIEWVEGDALNLPFSDGYFDAITMGYGLR 180

Query: 224 NVVDKRKALEEMLRVLKEGSKVSILDFNKTTQPITAAVQEWMINSIVVPVASGY------ 283
           NVVDKRKALEEMLRVLKEGSKVSILDFNKTTQP  AA+QEWMIN+IVVPVASGY      
Sbjct: 181 NVVDKRKALEEMLRVLKEGSKVSILDFNKTTQPTAAAIQEWMINNIVVPVASGYGLAEDY 240

Query: 284 ------------GKELEELALEVGFSTSTHYEISGGLMGNLVASR 311
                       GKELEELALEVGFSTS HYEISGGLMGNLVASR
Sbjct: 241 KYLSKSIKEFFTGKELEELALEVGFSTSIHYEISGGLMGNLVASR 257

BLAST of Spg025476 vs. ExPASy TrEMBL
Match: A0A6J1HDD3 (2-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111462490 PE=3 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 9.9e-111
Identity = 213/285 (74.74%), Postives = 228/285 (80.00%), Query Frame = 0

Query: 44  MASSLQFQLRLPSSTTPPLLRHRPIQCASDRQALFNRIAPVYDNLNDLLSLGQHRIWKRM 103
           MASSLQ Q RL  STTP LLRHRPIQCA+DR+ALF+RIAPVYDNLNDLLSLGQHRIWKRM
Sbjct: 1   MASSLQLQFRLSPSTTPALLRHRPIQCATDRRALFDRIAPVYDNLNDLLSLGQHRIWKRM 60

Query: 104 AVSWSGYVIFSFSDAELSCLLFKIFLTFRTFGNSAKAGDCVLDICCGSGDLAFLLSEKVG 163
           AVSWSG                            AKAGDCVLDICCGSGDLAFLLS+KVG
Sbjct: 61  AVSWSG----------------------------AKAGDCVLDICCGSGDLAFLLSQKVG 120

Query: 164 PDGKVTGLDFSREQLSIASSRQNSLSKPCYKNIEWMEGDALNLPFSDGSFDAITMGYGLR 223
            +GKVTGLDFSR+QLSIASSRQ SLS+PCYKNIEW+EGDALNLPFSDGS DAITMGYGLR
Sbjct: 121 SNGKVTGLDFSRQQLSIASSRQTSLSRPCYKNIEWVEGDALNLPFSDGSVDAITMGYGLR 180

Query: 224 NVVDKRKALEEMLRVLKEGSKVSILDFNKTTQPITAAVQEWMINSIVVPVASGY------ 283
           NV+DKRKALEEMLRVLKEGSK+SILDF+K TQP TA++QEWMIN+IVVPVASGY      
Sbjct: 181 NVLDKRKALEEMLRVLKEGSKLSILDFSKATQPTTASIQEWMINNIVVPVASGYGLAEDY 240

Query: 284 ------------GKELEELALEVGFSTSTHYEISGGLMGNLVASR 311
                       GKELE+LALEVGFSTS HYEISGGLMGNLVASR
Sbjct: 241 KYLSKSISEFSTGKELEKLALEVGFSTSIHYEISGGLMGNLVASR 257

BLAST of Spg025476 vs. TAIR 10
Match: AT1G23360.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 302.0 bits (772), Expect = 5.6e-82
Identity = 154/265 (58.11%), Postives = 187/265 (70.57%), Query Frame = 0

Query: 64  RHRPIQCASDRQALFNRIAPVYDNLNDLLSLGQHRIWKRMAVSWSGYVIFSFSDAELSCL 123
           R   ++C+++R+ LFNRIAPVYDNLNDLLSLGQHRIWK MAVSWSG              
Sbjct: 25  RRTVVKCSNERRILFNRIAPVYDNLNDLLSLGQHRIWKNMAVSWSG-------------- 84

Query: 124 LFKIFLTFRTFGNSAKAGDCVLDICCGSGDLAFLLSEKVGPDGKVTGLDFSREQLSIASS 183
                         AK GD VLD+CCGSGDLAFLLSEKVG  GKV GLDFS EQL++A++
Sbjct: 85  --------------AKKGDYVLDLCCGSGDLAFLLSEKVGSTGKVMGLDFSSEQLAVAAT 144

Query: 184 RQNSLSKPCYKNIEWMEGDALNLPFSDGSFDAITMGYGLRNVVDKRKALEEMLRVLKEGS 243
           RQ+  ++ CYK IEW+EGDA++LPF D  FDA+TMGYGLRNVVD+ +A++EM RVLK GS
Sbjct: 145 RQSLKARSCYKCIEWIEGDAIDLPFDDCEFDAVTMGYGLRNVVDRLRAMKEMYRVLKPGS 204

Query: 244 KVSILDFNKTTQPITAAVQEWMINSIVVPVASGY------------------GKELEELA 303
           +VSILDFNK+ Q +T  +Q WMI+++VVPVA+ Y                  G+ELE LA
Sbjct: 205 RVSILDFNKSNQSVTTFMQGWMIDNVVVPVATVYDLAKEYEYLKYSINGYLTGEELETLA 261

Query: 304 LEVGFSTSTHYEISGGLMGNLVASR 311
           LE GFS++ HYEISGG MGNLVA R
Sbjct: 265 LEAGFSSACHYEISGGFMGNLVAMR 261

BLAST of Spg025476 vs. TAIR 10
Match: AT1G23360.3 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 186.4 bits (472), Expect = 3.4e-47
Identity = 93/159 (58.49%), Postives = 119/159 (74.84%), Query Frame = 0

Query: 170 GLDFSREQLSIASSRQNSLSKPCYKNIEWMEGDALNLPFSDGSFDAITMGYGLRNVVDKR 229
           GLDFS EQL++A++RQ+  ++ CYK IEW+EGDA++LPF D  FDA+TMGYGLRNVVD+ 
Sbjct: 2   GLDFSSEQLAVAATRQSLKARSCYKCIEWIEGDAIDLPFDDCEFDAVTMGYGLRNVVDRL 61

Query: 230 KALEEMLRVLKEGSKVSILDFNKTTQPITAAVQEWMINSIVVPVASGY------------ 289
           +A++EM RVLK GS+VSILDFNK+ Q +T  +Q WMI+++VVPVA+ Y            
Sbjct: 62  RAMKEMYRVLKPGSRVSILDFNKSNQSVTTFMQGWMIDNVVVPVATVYDLAKEYEYLKYS 121

Query: 290 ------GKELEELALEVGFSTSTHYEISGGLMGNLVASR 311
                 G+ELE LALE GFS++ HYEISGG MGNLVA R
Sbjct: 122 INGYLTGEELETLALEAGFSSACHYEISGGFMGNLVAMR 160

BLAST of Spg025476 vs. TAIR 10
Match: AT1G23360.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 186.4 bits (472), Expect = 3.4e-47
Identity = 93/159 (58.49%), Postives = 119/159 (74.84%), Query Frame = 0

Query: 170 GLDFSREQLSIASSRQNSLSKPCYKNIEWMEGDALNLPFSDGSFDAITMGYGLRNVVDKR 229
           GLDFS EQL++A++RQ+  ++ CYK IEW+EGDA++LPF D  FDA+TMGYGLRNVVD+ 
Sbjct: 2   GLDFSSEQLAVAATRQSLKARSCYKCIEWIEGDAIDLPFDDCEFDAVTMGYGLRNVVDRL 61

Query: 230 KALEEMLRVLKEGSKVSILDFNKTTQPITAAVQEWMINSIVVPVASGY------------ 289
           +A++EM RVLK GS+VSILDFNK+ Q +T  +Q WMI+++VVPVA+ Y            
Sbjct: 62  RAMKEMYRVLKPGSRVSILDFNKSNQSVTTFMQGWMIDNVVVPVATVYDLAKEYEYLKYS 121

Query: 290 ------GKELEELALEVGFSTSTHYEISGGLMGNLVASR 311
                 G+ELE LALE GFS++ HYEISGG MGNLVA R
Sbjct: 122 INGYLTGEELETLALEAGFSSACHYEISGGFMGNLVAMR 160

BLAST of Spg025476 vs. TAIR 10
Match: AT5G57300.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 79.3 bits (194), Expect = 5.9e-15
Identity = 55/196 (28.06%), Postives = 83/196 (42.35%), Query Frame = 0

Query: 77  LFNRIAPVYDNLNDLLSLGQHRIWKRMAVSWSGYVIFSFSDAELSCLLFKIFLTFRTFGN 136
           +F  +A  YD +ND++S G HR+WK   V                    K+         
Sbjct: 48  VFTNVASSYDIMNDVMSGGLHRLWKERLVG-------------------KL--------- 107

Query: 137 SAKAGDCVLDICCGSGDLAFLLSEKVG---------------PDGKVTGLDFSREQLSIA 196
           S  AG   LD+  G+GD+AF + + V                 + ++   D +   L++ 
Sbjct: 108 SPFAGMKHLDVAGGTGDVAFRIYDAVYSVKRRALQKVDEASLEETQIYVCDINPNMLNVG 167

Query: 197 SSRQNSLSKPCYKNIEWMEGDALNLPFSDGSFDAITMGYGLRNVVDKRKALEEMLRVLKE 256
             R         K++ W+EGDA  L F D S D  T+ +G+RNV    KAL E  RVLK 
Sbjct: 168 KQRAAERGLRDNKSLVWVEGDAEALSFDDNSMDGYTIAFGIRNVTHIEKALAEAYRVLKR 215

Query: 257 GSKVSILDFNKTTQPI 258
           G +   L+ +    P+
Sbjct: 228 GGRFLCLELSHVEIPV 215

BLAST of Spg025476 vs. TAIR 10
Match: AT5G57300.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 79.3 bits (194), Expect = 5.9e-15
Identity = 55/196 (28.06%), Postives = 83/196 (42.35%), Query Frame = 0

Query: 77  LFNRIAPVYDNLNDLLSLGQHRIWKRMAVSWSGYVIFSFSDAELSCLLFKIFLTFRTFGN 136
           +F  +A  YD +ND++S G HR+WK   V                    K+         
Sbjct: 48  VFTNVASSYDIMNDVMSGGLHRLWKERLVG-------------------KL--------- 107

Query: 137 SAKAGDCVLDICCGSGDLAFLLSEKVG---------------PDGKVTGLDFSREQLSIA 196
           S  AG   LD+  G+GD+AF + + V                 + ++   D +   L++ 
Sbjct: 108 SPFAGMKHLDVAGGTGDVAFRIYDAVYSVKRRALQKVDEASLEETQIYVCDINPNMLNVG 167

Query: 197 SSRQNSLSKPCYKNIEWMEGDALNLPFSDGSFDAITMGYGLRNVVDKRKALEEMLRVLKE 256
             R         K++ W+EGDA  L F D S D  T+ +G+RNV    KAL E  RVLK 
Sbjct: 168 KQRAAERGLRDNKSLVWVEGDAEALSFDDNSMDGYTIAFGIRNVTHIEKALAEAYRVLKR 215

Query: 257 GSKVSILDFNKTTQPI 258
           G +   L+ +    P+
Sbjct: 228 GGRFLCLELSHVEIPV 215

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022951506.14.5e-11877.662-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic-like isoform X... [more]
XP_023002763.11.7e-11776.982-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic-like [Cucurbit... [more]
XP_022951505.12.5e-11676.612-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic-like isoform X... [more]
XP_038885206.11.2e-11377.542-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic [Benincasa his... [more]
XP_023545823.12.2e-11275.792-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic isoform X3 [Cu... [more]
Match NameE-valueIdentityDescription
Q3ED657.9e-8158.112-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic OS=Arabidopsis... [more]
B2IUM73.1e-5346.672-phytyl-1,4-naphtoquinone methyltransferase OS=Nostoc punctiforme (strain ATCC ... [more]
Q8YLP49.0e-5344.312-phytyl-1,4-naphtoquinone methyltransferase OS=Nostoc sp. (strain PCC 7120 / SA... [more]
Q3MD911.0e-5143.922-phytyl-1,4-naphtoquinone methyltransferase OS=Trichormus variabilis (strain AT... [more]
P728181.6e-4943.702-phytyl-1,4-naphtoquinone methyltransferase OS=Synechocystis sp. (strain PCC 68... [more]
Match NameE-valueIdentityDescription
A0A6J1GHS22.2e-11877.662-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic OS=Cucurbita m... [more]
A0A6J1KUI98.3e-11876.982-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic OS=Cucurbita m... [more]
A0A6J1GIZ31.2e-11676.612-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic OS=Cucurbita m... [more]
A0A6J1BRH72.0e-11176.492-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic OS=Momordica c... [more]
A0A6J1HDD39.9e-11174.742-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic OS=Cucurbita m... [more]
Match NameE-valueIdentityDescription
AT1G23360.15.6e-8258.11S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G23360.33.4e-4758.49S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G23360.23.4e-4758.49S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G57300.15.9e-1528.06S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G57300.25.9e-1528.06S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 80..310
e-value: 1.5E-48
score: 167.2
NoneNo IPR availablePANTHERPTHR43591:SF692-PHYTYL-1,4-BETA-NAPHTHOQUINONE METHYLTRANSFERASE, CHLOROPLASTICcoord: 60..109
coord: 137..310
NoneNo IPR availablePANTHERPTHR43591METHYLTRANSFERASEcoord: 60..109
coord: 137..310
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 144..247
e-value: 4.88293E-14
score: 65.1438
IPR004033UbiE/COQ5 methyltransferasePFAMPF01209Ubie_methyltrancoord: 136..267
e-value: 2.8E-35
score: 121.7
coord: 75..105
e-value: 4.9E-6
score: 26.0
IPR004033UbiE/COQ5 methyltransferasePROSITEPS51608SAM_MT_UBIEcoord: 69..310
score: 39.578808
IPR023576UbiE/COQ5 methyltransferase, conserved sitePROSITEPS01183UBIE_1coord: 85..100
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 75..301

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg025476.1Spg025476.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0042372 phylloquinone biosynthetic process
biological_process GO:0006744 ubiquinone biosynthetic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0052624 2-phytyl-1,4-naphthoquinone methyltransferase activity
molecular_function GO:0008168 methyltransferase activity