Spg025447 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg025447
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	abscisic acid 8'-hydroxylase 4-like
Location	scaffold13: 39626229 .. 39630354 (-)
RNA-Seq Expression	Spg025447
Synteny	Spg025447

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTCTTCAATTCCTCCACACAAAAAAATGATGCCTCAAAATTTTCTACTCATTTCCATTTTTCCTCTAGCCCTCCTCCTTCTTCTACCTCTAATTTTCTTTCATGTCCAAAAGAACAAGAATATTGCAAAGAAGCTCCCTCCTGGCTCAATGGGCTGGCCCTTAATCGGCGAGACCTTTCGTCTCTACTCTCAACATCCATCCTCCTTCTTCCTTGGAAAACAAAAAAGGTTGTCCTTTCAACTACTAATAATTCCCATCTTGCTCTCTCTCGAGTACACGAAAAACCCTTTTTTCGAAAAGAAAATTATTTAAGGAACTAAAATAATACGAGATAATTAATTGCGTTGAATAATTTTTGTTCTGATGCAGGTATGGGGATATATTCAAGACCCACATTCTTGGGTGTCCGTGTGTGATGTTGGCGACTCCCGAGGCGGCTCGGTTCGTGCTAGTGACGCAGGCTCATCTCTTCAAACCCACATACCCACAAAGCAAAGAGCGTCTAATCGGACCATCGGCTCTGTTTTTCCACCAAAGCGAAACCCATTTGCGGCTGCGTAAGCTAATCCAAAGCTCCCTCTCACCGGACCCTGTCCGAGCCCTGGTCCCCGACATCCACGCCCTCGCCGTCTCTACATTGCCGGCATGGCTCAATGGCCATCTCATCAACACTTTCCATCAAATGAAGAAGGTAAATTTCAATTTTGTTTTTTTAAAAAAATCTAAATTTCACTTGGGGCAGAGTTGCTCTGTTAACAAAGACAAAAACAGAGCTTTCAAAAAGTTTGTGTTCTTAATTGAATCAAATGGGGACCAATTATTCCTTATCAATTGTTTAATTTTGGTATCTTCTTCTTCTCTCTCTGTCTCGCTGACTTCTATAGCCTTTGTTGTCGTTGTGTCTTTTTTCTTCACTCTCCAAAGTTCTCTTTTGAAGTCGGAATTTTGGCGATTTTTGGGCATGTGGAGGAGGCCAATTACAGAGGCGAATTACTGAGAAACTATTCCATAGTGAACAAAGGCTACAATTCTTTTCCAACCAACATTCCCGGAACGCTTTACAAAAAAGCACTTAAAGTAAGTCAAAAAACACACACATACAACATTCGGATTCTCGAGTGAATTATTGTTTGTTTGTTCTAAGAATCTGTTTGCCACTTTTCTGTAAAGGCGAGAAAGAGGCTGGGAGAGATCATAAGCAGTATAATCTCAGAGAAGAGAAAGGAATTGGTGGAGAAGAATTTGTTGGGTTGTTTGCTAAAATGGAGAGATGAAAATGGAGAAAGTCTAAGTGATGAACAGATTGCTGATAATATAATTGGAGTTCTGTTTGCTGCTCAGGACACCACTGCCAGTGTTCTGACATGGGTTGTCAAATATCTCCATGATAATCAGAAAGTTCTAGAAGCTGTCAAGGTACTCATGTTCTTTTTTCTTTTTGGACCATATATAAGTTACTAACACTTTTTTGTTGAGGTGAATTTTTAGTCATTAAGAATAAAATTAGAGTATAGTTTGAAAAAAAGAAAGAAGGATGTGTTTATTGAGTGAATGCAATGGAAATAGATCATTAAGATAACAAGTATATGTATTTATTCGCCAACAAGAACTTAGCTCAACTGGCATAAAGTTGTACTGATAACCACAAAGTCTGTGGTTCGACCAAATGTCTAATCTATTTAGTTATGTTTGGACCCATGTTATTGTAGGTGGAACAAAAGGCAATTGAAGAATGTATTAATGAGGGGAGCCGACCAATGAGTTGGACTCAGACACGAAACATGCCGTTTACCAACAAGGTAGGAAGTTGGAGATTATTTATTTTTTATCTTTTATTTTTTTGAAAAGAGATGCTGGAGAAGTTAAACAAGTGTTTTTGAGAGATGGATCAAAGTCAAAATTGGGCCTTTTTGTTTTCTATTTTTCTTCTTTTTTGTTTTTTAAAGTTAATGTATTGATTGATTGAGCTATTTGTCGGTTACATTTAAAAAATAAAAATAAAAACAGGTGGTGTTGGAGAGCTTGAGAATGGCGAGTATTATATCATTCACTTTTAGGGAAGCTGTGGCAGATGTTGAATACAATGGTAAGACGCTAAAAATAACTCTAAATTAACACTAACATGTAAATGCACCTTTCACTTGTATTTCACTTGTGTTTGTTAGTTTGTTTGAATGTCCTTTTAAAATTAATAAAATATTTTGATATTTGGTTCTATATTTATGTAAATGTACTTGCTCCATGTTTTTTCACAAAGCACTAGTTACAAGCACTTTTTTTATTACTACCCTAATAGAGGTTCCTTTTTGTTACTTTCATGTATAGTTTATTTCCCTCGGCAATTTCTATTAAAAATTCTATTTTATTAGATAAGTGGATTCTACTAATATTTTTTAACTACTACAAATAAATAAAATAAATAATTTGAATAGGAACTCTAACGGATCATATTTAGATCATATTACTTGAAAAATATTATGATTTTAAGTTGGAAATGTTTTTTTTTTTTTTTGGTCCTTTTTTAAAAAAAAAAAAAAAATCAGGATAAGAAAAGGAAAAAAAAAGATTCTTGAGATACTTGTAAAAAGATGGGTTAAATTTTGCAGCCCCCTCCCCCAAAAGTTTAATCTTTTACATATGGAATTTTAGGTCAAACTTAGTTGGAAAGCTATCTTTTTTTTATTAAATTTTTTGTTTTTGGTTGGAAGTTTTGGTTTAAAATAAGTGATTCCTTACTTTTATTATTTTTTTTTTATTTTAAATTTTGAGCAGGATATTTAATTCCAAAGGGTTGGAAAGTAATGCCTTTGTTCAGGAATATTCATCACAATCCTGACTACTTTGTTGACCCTCACAAATTTGATCCTTCAAGATTTGAGGTATATTTGAATTTCCCTATACTTTCTTTTCTTTTTTGTCTTATCATCCCAAAAAGAAAAATTCTTTTCTTTTCTTTTCTCTATGTTTGATGTTAATGTTACCTACAAAAATATATTCAGCTATGATTTTTAATGAAAAGACAAGCTTATTTTATGTGAGAACAGAAATTGCAAGTAATATGGACAACTATACCTATCTTTCACTTTCAACCTCCCAATTTTTGAACTAAACAAAAAAAAAAACCTCCCAATTTTTTTTTGCTCATTTTCAATTTAATTATCATGATTTAAGTTTTTTTTTTTTTACACCACAATTGAAGATTAGGTTAGGGATATGAATATAGTTTCAAATCCATTAGAAGAAATTGAAAAGATTTAAAGATACTTTAGATTGAAGTATTTCACAAAGATACTTTTGTTATATCTGTTATTAGAGTACTTCTATGTAAAAAGAAGATATTTTTATGGCTTAGACTAACAATTTATGTGCATCTTAAAATATTTGTTCAATATTATCTAACTAAAAAAGGGATCTATCAGGTTGCTCCAAGACCCAATACATTCATGCCATTTGGCGGTGGGGTCCATGCCTGCCCTGGAAATGAGCTTGCCAAGCTTGAAATCTTGATCATGATTCATCATCTAGTCACCAAATTCAGGTCCTTATTTTTCTCTTTTTTTCTTTTTTAGAATATAGCATCACAAAGAAATTCAAATTCTCTGATTTATAAAAAATGTGATAACTTGGATATAGATAAAACACTTATTATCATTTTAAAGGTCGATTGTTTGATTGTTCAAATTGATATCGACTCCGGCCGTGTTTGGTTGCAGGTGGGAATTGGCAGGAGAGAGAGATGGGATTCAGCATGGGCCATTTCCAGTGCCTATGGATGGGCTTCCAATCAGAGTCTGGAGATTATCCACATGAAGTGGGCCTCATTTCACTAAAGCCCAATTTGGGCCCTCTTAAATGAAAATGGGCCTCATTTCAGTAAGCCCAAAAAGGGTCTCTCACTGAAATGTTTTTTTTTTTTTGGTCGTTGTGCAATTGGAGTTGGCCATTAAGGCGAGTGTTCAGTTAGGGATCCATCCACCCCCAAGGAAATGTGGTGGCTTTGGTGATATAATTTTTTTTTTTTTTTTTTTTTTGCAAAGCCATGTAATATTCTACCAAGCTCTTCAACAAAAGTATTAACAGAGCATAGTTTCTCCTAAAGGAGAAAATTTTCCAATCACATAATA

mRNA sequence

ATGATGCCTCAAAATTTTCTACTCATTTCCATTTTTCCTCTAGCCCTCCTCCTTCTTCTACCTCTAATTTTCTTTCATGTCCAAAAGAACAAGAATATTGCAAAGAAGCTCCCTCCTGGCTCAATGGGCTGGCCCTTAATCGGCGAGACCTTTCGTCTCTACTCTCAACATCCATCCTCCTTCTTCCTTGGAAAACAAAAAAGGTATGGGGATATATTCAAGACCCACATTCTTGGGTGTCCGTGTGTGATGTTGGCGACTCCCGAGGCGGCTCGGTTCGTGCTAGTGACGCAGGCTCATCTCTTCAAACCCACATACCCACAAAGCAAAGAGCGTCTAATCGGACCATCGGCTCTGTTTTTCCACCAAAGCGAAACCCATTTGCGGCTGCGTAAGCTAATCCAAAGCTCCCTCTCACCGGACCCTGTCCGAGCCCTGGTCCCCGACATCCACGCCCTCGCCGTCTCTACATTGCCGGCATGGCTCAATGGCCATCTCATCAACACTTTCCATCAAATGAAGAAGTTCTCTTTTGAAGTCGGAATTTTGGCGATTTTTGGGCATGTGGAGGAGGCCAATTACAGAGGCGAATTACTGAGAAACTATTCCATAGTGAACAAAGGCTACAATTCTTTTCCAACCAACATTCCCGGAACGCTTTACAAAAAAGCACTTAAAGCGAGAAAGAGGCTGGGAGAGATCATAAGCAGTATAATCTCAGAGAAGAGAAAGGAATTGGTGGAGAAGAATTTGTTGGGTTGTTTGCTAAAATGGAGAGATGAAAATGGAGAAAGTCTAAGTGATGAACAGATTGCTGATAATATAATTGGAGTTCTGTTTGCTGCTCAGGACACCACTGCCAGTGTTCTGACATGGGTTGTCAAATATCTCCATGATAATCAGAAAGTTCTAGAAGCTGTCAAGGTGGTGTTGGAGAGCTTGAGAATGGCGAGTATTATATCATTCACTTTTAGGGAAGCTGTGGCAGATGTTGAATACAATGGATATTTAATTCCAAAGGGTTGGAAAGTAATGCCTTTGTTCAGGAATATTCATCACAATCCTGACTACTTTGTTGACCCTCACAAATTTGATCCTTCAAGATTTGAGGTTGCTCCAAGACCCAATACATTCATGCCATTTGGCGGTGGGGTCCATGCCTGCCCTGGAAATGAGCTTGCCAAGCTTGAAATCTTGATCATGATTCATCATCTAGTCACCAAATTCAGGTGGGAATTGGCAGGAGAGAGAGATGGGATTCAGCATGGGCCATTTCCAGTGCCTATGGATGGGCTTCCAATCAGAGTCTGGAGATTATCCACATGA

Coding sequence (CDS)

ATGATGCCTCAAAATTTTCTACTCATTTCCATTTTTCCTCTAGCCCTCCTCCTTCTTCTACCTCTAATTTTCTTTCATGTCCAAAAGAACAAGAATATTGCAAAGAAGCTCCCTCCTGGCTCAATGGGCTGGCCCTTAATCGGCGAGACCTTTCGTCTCTACTCTCAACATCCATCCTCCTTCTTCCTTGGAAAACAAAAAAGGTATGGGGATATATTCAAGACCCACATTCTTGGGTGTCCGTGTGTGATGTTGGCGACTCCCGAGGCGGCTCGGTTCGTGCTAGTGACGCAGGCTCATCTCTTCAAACCCACATACCCACAAAGCAAAGAGCGTCTAATCGGACCATCGGCTCTGTTTTTCCACCAAAGCGAAACCCATTTGCGGCTGCGTAAGCTAATCCAAAGCTCCCTCTCACCGGACCCTGTCCGAGCCCTGGTCCCCGACATCCACGCCCTCGCCGTCTCTACATTGCCGGCATGGCTCAATGGCCATCTCATCAACACTTTCCATCAAATGAAGAAGTTCTCTTTTGAAGTCGGAATTTTGGCGATTTTTGGGCATGTGGAGGAGGCCAATTACAGAGGCGAATTACTGAGAAACTATTCCATAGTGAACAAAGGCTACAATTCTTTTCCAACCAACATTCCCGGAACGCTTTACAAAAAAGCACTTAAAGCGAGAAAGAGGCTGGGAGAGATCATAAGCAGTATAATCTCAGAGAAGAGAAAGGAATTGGTGGAGAAGAATTTGTTGGGTTGTTTGCTAAAATGGAGAGATGAAAATGGAGAAAGTCTAAGTGATGAACAGATTGCTGATAATATAATTGGAGTTCTGTTTGCTGCTCAGGACACCACTGCCAGTGTTCTGACATGGGTTGTCAAATATCTCCATGATAATCAGAAAGTTCTAGAAGCTGTCAAGGTGGTGTTGGAGAGCTTGAGAATGGCGAGTATTATATCATTCACTTTTAGGGAAGCTGTGGCAGATGTTGAATACAATGGATATTTAATTCCAAAGGGTTGGAAAGTAATGCCTTTGTTCAGGAATATTCATCACAATCCTGACTACTTTGTTGACCCTCACAAATTTGATCCTTCAAGATTTGAGGTTGCTCCAAGACCCAATACATTCATGCCATTTGGCGGTGGGGTCCATGCCTGCCCTGGAAATGAGCTTGCCAAGCTTGAAATCTTGATCATGATTCATCATCTAGTCACCAAATTCAGGTGGGAATTGGCAGGAGAGAGAGATGGGATTCAGCATGGGCCATTTCCAGTGCCTATGGATGGGCTTCCAATCAGAGTCTGGAGATTATCCACATGA

Protein sequence

MMPQNFLLISIFPLALLLLLPLIFFHVQKNKNIAKKLPPGSMGWPLIGETFRLYSQHPSSFFLGKQKRYGDIFKTHILGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPDIHALAVSTLPAWLNGHLINTFHQMKKFSFEVGILAIFGHVEEANYRGELLRNYSIVNKGYNSFPTNIPGTLYKKALKARKRLGEIISSIISEKRKELVEKNLLGCLLKWRDENGESLSDEQIADNIIGVLFAAQDTTASVLTWVVKYLHDNQKVLEAVKVVLESLRMASIISFTFREAVADVEYNGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPSRFEVAPRPNTFMPFGGGVHACPGNELAKLEILIMIHHLVTKFRWELAGERDGIQHGPFPVPMDGLPIRVWRLST

Homology

[Close]

BLAST of Spg025447 vs. NCBI nr
Match: XP_038884199.1 (abscisic acid 8'-hydroxylase CYP707A1-like [Benincasa hispida])

HSP 1 Score: 771.2 bits (1990), Expect = 5.0e-219
Identity = 389/470 (82.77%), Postives = 402/470 (85.53%), Query Frame = 0


Query: 6   FLLISIFPLALLLLLPLIFFHVQKNKNIA-KKLPPGSMGWPLIGETFRLYSQHPSSFFLG 65
           FLLIS F L LL    L FF + KN     KKLPPGSMGWPLIGETF  YSQ P+SFFLG
Sbjct: 5   FLLISFFLLPLL----LFFFQIHKNSKFPNKKLPPGSMGWPLIGETFHFYSQDPTSFFLG 64

Query: 66  KQKRYGDIFKTHILGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFHQS 125
           KQKRYG+IFKTHI GCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFHQS
Sbjct: 65  KQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFHQS 124

Query: 126 ETHLRLRKLIQSSLSPDPVRALVPDIHALAVSTLPAWLNGHLINTFHQMKKFSFEVGILA 185
           ETHLRLRKLIQSSLSPDPVRALVPDIHALA+S LP+WLNGHLINTFHQMKKFSFEVGILA
Sbjct: 125 ETHLRLRKLIQSSLSPDPVRALVPDIHALAISALPSWLNGHLINTFHQMKKFSFEVGILA 184

Query: 186 IFGHVEEANYRGELLRNYSIVNKGYNSFPTNIPGTLYKKALKARKRLGEIISSII---SE 245
           IFGH+E+  YR EL+ NYSIVNKGYNSFPTNIPGTLYKKALKARKRLGEIIS II    E
Sbjct: 185 IFGHLEDTKYREELMTNYSIVNKGYNSFPTNIPGTLYKKALKARKRLGEIISCIILEKRE 244

Query: 246 KRKELVEKNLLGCLLKWRDENGESLSDEQIADNIIGVLFAAQDTTASVLTWVVKYLHDNQ 305
           KRK+L EKNLL CLLKWRDE GE+LSDEQIADNIIGVLFAAQDTTASVLTWVVKYLH N 
Sbjct: 245 KRKQLEEKNLLACLLKWRDEKGETLSDEQIADNIIGVLFAAQDTTASVLTWVVKYLHYNH 304

Query: 306 KVLEAV------------------------------KVVLESLRMASIISFTFREAVADV 365
           KVLEAV                              KVVLES RMASIISFTFREAVADV
Sbjct: 305 KVLEAVKVEQKAIEEGINEGCRPMSWSQTRNMPFTNKVVLESFRMASIISFTFREAVADV 364

Query: 366 EYNGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPSRFEVAPRPNTFMPFGGGVHACPGN 425
           EY GYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPSRFEVAPRPNTFMPFG GVHACPGN
Sbjct: 365 EYKGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPSRFEVAPRPNTFMPFGSGVHACPGN 424

Query: 426 ELAKLEILIMIHHLVTKFRWELAGERDGIQHGPFPVPMDGLPIRVWRLST 442
           ELAKLEILIMIHHLVTKFRWELA +RDGIQHGPFPVP++GLPIRVWRLST
Sbjct: 425 ELAKLEILIMIHHLVTKFRWELAEQRDGIQHGPFPVPINGLPIRVWRLST 470

[Close]

BLAST of Spg025447 vs. NCBI nr
Match: XP_023546528.1 (abscisic acid 8'-hydroxylase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 742.3 bits (1915), Expect = 2.5e-210
Identity = 373/474 (78.69%), Postives = 397/474 (83.76%), Query Frame = 0


Query: 2   MPQNFLLISI-FPLALLLLLPLIFFHVQKNKNIA-KKLPPGSMGWPLIGETFRLYSQHPS 61
           M   F+LISI FPL L       F+ + KN  I  KKLPPGSMGWPLIGETF  YSQ   
Sbjct: 4   MAATFVLISILFPLLL-----CFFWKIHKNNRIPNKKLPPGSMGWPLIGETFHFYSQ-DL 63

Query: 62  SFFLGKQKRYGDIFKTHILGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSAL 121
           +FF  KQKRYG+IFKTHI GCPCVML TPEAARFVLVTQAHLFKPTYPQSKERLIGPSAL
Sbjct: 64  TFFFRKQKRYGEIFKTHIHGCPCVMLTTPEAARFVLVTQAHLFKPTYPQSKERLIGPSAL 123

Query: 122 FFHQSETHLRLRKLIQSSLSPDPVRALVPDIHALAVSTLPAWLNGHLINTFHQMKKFSFE 181
           FFHQSETHLRLRKLIQSSLSPDPVR LVP IHALA+STLP+WLNGHLINT HQMKKFSFE
Sbjct: 124 FFHQSETHLRLRKLIQSSLSPDPVRTLVPHIHALAISTLPSWLNGHLINTLHQMKKFSFE 183

Query: 182 VGILAIFGHVEEANYRGELLRNYSIVNKGYNSFPTNIPGTLYKKALKARKRLGEIISSII 241
           VGIL IFGH+E ANYR ELL+NYSIVNKGYNSFPTN PG++YKKALKAR RLGEII+ II
Sbjct: 184 VGILVIFGHLENANYRDELLKNYSIVNKGYNSFPTNFPGSIYKKALKARNRLGEIINCII 243

Query: 242 SEK---RKELVEKNLLGCLLKWRDENGESLSDEQIADNIIGVLFAAQDTTASVLTWVVKY 301
           SEK   RKEL EKNLL CLLKWRDE GESLSD+QIADNIIGVLFAAQDTTASVLTWVVKY
Sbjct: 244 SEKREQRKELEEKNLLACLLKWRDEKGESLSDDQIADNIIGVLFAAQDTTASVLTWVVKY 303

Query: 302 LHDNQKVLEAV------------------------------KVVLESLRMASIISFTFRE 361
           LHDN K+LEAV                              KVVLESLRMA++ISF+FRE
Sbjct: 304 LHDNDKILEAVKVEQRAIQEAINEGSRAMSWADTRNMPFTNKVVLESLRMATVISFSFRE 363

Query: 362 AVADVEYNGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPSRFEVAPRPNTFMPFGGGVH 421
           AV DVEYNGYLIPKGWKVMPLFRNIHHNPDYF+ PHKFDPSRFEVAPRPNTF+PFG GVH
Sbjct: 364 AVTDVEYNGYLIPKGWKVMPLFRNIHHNPDYFLHPHKFDPSRFEVAPRPNTFIPFGSGVH 423

Query: 422 ACPGNELAKLEILIMIHHLVTKFRWELAGERDGIQHGPFPVPMDGLPIRVWRLS 441
           ACPGN+LAKLEILIMIHHLVTKFRWELAG++DGI+HGPFPVP DGLPIR+WRLS
Sbjct: 424 ACPGNDLAKLEILIMIHHLVTKFRWELAGQKDGIEHGPFPVPRDGLPIRLWRLS 471

[Close]

BLAST of Spg025447 vs. NCBI nr
Match: XP_022962256.1 (abscisic acid 8'-hydroxylase 3-like [Cucurbita moschata])

HSP 1 Score: 741.5 bits (1913), Expect = 4.2e-210
Identity = 372/473 (78.65%), Postives = 396/473 (83.72%), Query Frame = 0


Query: 2   MPQNFLLISIFPLALLLLLPLIFFHVQKNKNIA-KKLPPGSMGWPLIGETFRLYSQHPSS 61
           M   F+LISIF      LL   F+ + KN  I  KKLPPGSMGWPLIGETF  YSQ   +
Sbjct: 1   MAATFVLISIF----FPLLLCFFWKIHKNNRIPNKKLPPGSMGWPLIGETFHFYSQ-DLT 60

Query: 62  FFLGKQKRYGDIFKTHILGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALF 121
           FF  KQKRYG+IFKTHI GCPCVML TPEAARFVLVTQAHLFKPTYPQSKERLIGPSALF
Sbjct: 61  FFFRKQKRYGEIFKTHIHGCPCVMLTTPEAARFVLVTQAHLFKPTYPQSKERLIGPSALF 120

Query: 122 FHQSETHLRLRKLIQSSLSPDPVRALVPDIHALAVSTLPAWLNGHLINTFHQMKKFSFEV 181
           FHQSETHLRLRKLIQSSLSPDPVR LVP IHALA+STLP+WLNGHLINT HQMKKFSFEV
Sbjct: 121 FHQSETHLRLRKLIQSSLSPDPVRTLVPHIHALAISTLPSWLNGHLINTLHQMKKFSFEV 180

Query: 182 GILAIFGHVEEANYRGELLRNYSIVNKGYNSFPTNIPGTLYKKALKARKRLGEIISSIIS 241
           GIL IFGH+E ANYR ELL+NYSIVNKGYNSFPTN PG++YKKALKAR RLGEII+ IIS
Sbjct: 181 GILVIFGHLENANYRDELLKNYSIVNKGYNSFPTNFPGSIYKKALKARNRLGEIINCIIS 240

Query: 242 EK---RKELVEKNLLGCLLKWRDENGESLSDEQIADNIIGVLFAAQDTTASVLTWVVKYL 301
           EK   RKEL EKNLL CLLKWRDE GESLSD+QIADNIIGVLFAAQDTTASVLTWVVKYL
Sbjct: 241 EKREQRKELEEKNLLACLLKWRDEKGESLSDDQIADNIIGVLFAAQDTTASVLTWVVKYL 300

Query: 302 HDNQKVLEAV------------------------------KVVLESLRMASIISFTFREA 361
           HDN K+LEAV                              KVVLESLRMA++ISF+FREA
Sbjct: 301 HDNDKILEAVKAEQRAIQEAINEGSRAMSWADTRNMPFTNKVVLESLRMATVISFSFREA 360

Query: 362 VADVEYNGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPSRFEVAPRPNTFMPFGGGVHA 421
           V DVEYNGYLIPKGWKVMPLFRNIHHNPDYF+ PHKFDPSRFEVAPRPNTF+PFG GVHA
Sbjct: 361 VTDVEYNGYLIPKGWKVMPLFRNIHHNPDYFLHPHKFDPSRFEVAPRPNTFIPFGSGVHA 420

Query: 422 CPGNELAKLEILIMIHHLVTKFRWELAGERDGIQHGPFPVPMDGLPIRVWRLS 441
           CPGN+LAKLEILIMIHHLVTKFRWELAG++DGI+HGPFPVP DGLPIR+WRLS
Sbjct: 421 CPGNDLAKLEILIMIHHLVTKFRWELAGQKDGIEHGPFPVPRDGLPIRLWRLS 468

[Close]

BLAST of Spg025447 vs. NCBI nr
Match: XP_022144125.1 (abscisic acid 8'-hydroxylase 4-like [Momordica charantia])

HSP 1 Score: 738.4 bits (1905), Expect = 3.6e-209
Identity = 372/475 (78.32%), Postives = 399/475 (84.00%), Query Frame = 0


Query: 2   MPQNFLLISIF--PLALLLLLPLIFFHVQKNKNIAKKLPPGSMGWPLIGETFRLYSQHPS 61
           M + F+L SIF   L LLLLLPLIFF  + NK   KKLPPGSMGWPLIGETF LYS+ P+
Sbjct: 1   MGEIFVLFSIFLPTLLLLLLLPLIFFKKENNK--GKKLPPGSMGWPLIGETFHLYSRDPT 60

Query: 62  SFFLGKQKRYGDIFKTHILGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSAL 121
            FFL KQKRYG+IFKTHI GCPCVMLATPEAARFVLVTQAHLFKPTYP  KERLIGPSAL
Sbjct: 61  IFFLDKQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHLFKPTYPHGKERLIGPSAL 120

Query: 122 FFHQSETHLRLRKLIQSSLSPDPVRALVPDIHALAVSTLPAWLNGHLINTFHQMKKFSFE 181
           FFHQS+ HLRLRKL+QSSLSPDPVRALVP I ALA S L  WL+GH +NTF +MKKFSFE
Sbjct: 121 FFHQSDAHLRLRKLVQSSLSPDPVRALVPYIDALASSALAFWLHGHTVNTFEEMKKFSFE 180

Query: 182 VGILAIFGHVEEANYRGELLRNYSIVNKGYNSFPTNIPGTLYKKALKARKRLGEIISSII 241
           VGILAIFGH+E+ NYR EL++NYS+VN+GYNSF TNIPGT+YKKALKARKRLGEIIS II
Sbjct: 181 VGILAIFGHLEDDNYRDELMKNYSMVNRGYNSFLTNIPGTIYKKALKARKRLGEIISCII 240

Query: 242 SEKR----KELVEKNLLGCLLKWRDENGESLSDEQIADNIIGVLFAAQDTTASVLTWVVK 301
           SEKR    K+L EKNLLGCLLKWRDE GE+LSDEQIADN+IGVLFAAQDTTASVLTWV+K
Sbjct: 241 SEKRQNTTKKLAEKNLLGCLLKWRDEKGETLSDEQIADNLIGVLFAAQDTTASVLTWVLK 300

Query: 302 YLHDNQKVLEAV------------------------------KVVLESLRMASIISFTFR 361
           YLHDN+KVL+AV                              KVVLESLRMASII+FTFR
Sbjct: 301 YLHDNRKVLQAVKAEQKAIEEAINGGSRPMSWSHTRNMPFTNKVVLESLRMASIITFTFR 360

Query: 362 EAVADVEYNGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPSRFEVAPRPNTFMPFGGGV 421
           EAVADVEYNGYLIPKGWKVMPLF NIHHN +YFVDPHKFDPSRFEVAPRPNTFMPFG GV
Sbjct: 361 EAVADVEYNGYLIPKGWKVMPLFSNIHHNSEYFVDPHKFDPSRFEVAPRPNTFMPFGSGV 420

Query: 422 HACPGNELAKLEILIMIHHLVTKFRWELAGERDGIQHGPFPVPMDGLPIRVWRLS 441
           HACPGNELAKLEILIMIHHLVTKFRWE AG  DGI HGPFPVP+DGLPIRVWRLS
Sbjct: 421 HACPGNELAKLEILIMIHHLVTKFRWEFAGRGDGIHHGPFPVPLDGLPIRVWRLS 473

[Close]

BLAST of Spg025447 vs. NCBI nr
Match: KAA0060904.1 (abscisic acid 8'-hydroxylase 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 731.5 bits (1887), Expect = 4.4e-207
Identity = 361/434 (83.18%), Postives = 379/434 (87.33%), Query Frame = 0


Query: 42  MGWPLIGETFRLYSQHPSSFFLGKQKRYGDIFKTHILGCPCVMLATPEAARFVLVTQAHL 101
           MGWPLIGETF  YSQHP+SFFLGKQKRYG+IFKTHI GCPCVMLATPEAARFVLVTQAHL
Sbjct: 1   MGWPLIGETFHFYSQHPTSFFLGKQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHL 60

Query: 102 FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPDIHALAVSTLPAW 161
           FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVP IHALA+S LP+ 
Sbjct: 61  FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPHIHALAISALPSS 120

Query: 162 LNGHLINTFHQMKKFSFEVGILAIFGHVEEANYRGELLRNYSIVNKGYNSFPTNIPGTLY 221
           L+GH+I+T+HQMKKFSFEVGILAIFGH+E+ NYR EL  NYSIVNKGYNSFPTNIPGTLY
Sbjct: 121 LSGHVIDTYHQMKKFSFEVGILAIFGHLEDTNYREELRTNYSIVNKGYNSFPTNIPGTLY 180

Query: 222 KKALKARKRLGEIISSIISEKR----KELVEKNLLGCLLKWRDENGESLSDEQIADNIIG 281
           KKA+KARKRL EIIS II EK+    K L EKNLL CL+KWRDE GESLSDEQIADNIIG
Sbjct: 181 KKAIKARKRLEEIISCIILEKKEKRSKVLGEKNLLSCLVKWRDEKGESLSDEQIADNIIG 240

Query: 282 VLFAAQDTTASVLTWVVKYLHDNQKVLEAV------------------------------ 341
           VLFAAQDTTASVLTWVVKYLHDN KVLEAV                              
Sbjct: 241 VLFAAQDTTASVLTWVVKYLHDNPKVLEAVKVEQKVIEEDINEGSRPMRWSHTRNMPFTN 300

Query: 342 KVVLESLRMASIISFTFREAVADVEYNGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPS 401
           KVVLESLRMASIISFTFREAVADVEY GYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPS
Sbjct: 301 KVVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPS 360

Query: 402 RFEVAPRPNTFMPFGGGVHACPGNELAKLEILIMIHHLVTKFRWELAGERDGIQHGPFPV 442
           RFEVAPRPNTFMPFG GVH+CPGNELAKLEI IMIHHLVTKFRWELAG+RDGI+HGPFPV
Sbjct: 361 RFEVAPRPNTFMPFGSGVHSCPGNELAKLEIFIMIHHLVTKFRWELAGQRDGIEHGPFPV 420

[Close]

BLAST of Spg025447 vs. ExPASy Swiss-Prot
Match: A9QNE7 (Abscisic acid 8'-hydroxylase CYP707A1 OS=Solanum lycopersicum OX=4081 GN=CYP707A1 PE=2 SV=1)

HSP 1 Score: 603.2 bits (1554), Expect = 2.3e-171
Identity = 301/473 (63.64%), Postives = 362/473 (76.53%), Query Frame = 0


Query: 6   FLLISIFPLALLLLLPLIFFHVQKNKNI-----AKKLPPGSMGWPLIGETFRLYSQHPSS 65
           FL IS+F L  L      +F   KN N      A KLPPGSMGWP IGET +LYSQ P++
Sbjct: 8   FLYISMFVLGYL----SYYFCFGKNNNSSSKKNAYKLPPGSMGWPYIGETLQLYSQDPNA 67

Query: 66  FFLGKQKRYGDIFKTHILGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALF 125
           FF+ +Q+R+G+IFKT ILGCPCVMLA+PEAARFVLV QA+LFKPTYP+SKE LIG SA+F
Sbjct: 68  FFINRQRRFGEIFKTKILGCPCVMLASPEAARFVLVNQANLFKPTYPKSKENLIGQSAIF 127

Query: 126 FHQSETHLRLRKLIQSSLSPDPVRALVPDIHALAVSTLPAWLNGHLINTFHQMKKFSFEV 185
           FHQ + H  LRKL+Q+ L+P+ +R  +P I  L++S L +W+ GH++NT+H+MKKFSFEV
Sbjct: 128 FHQGDYHNHLRKLVQAPLNPESIRNQIPYIEELSISALNSWVGGHVVNTYHEMKKFSFEV 187

Query: 186 GILAIFGHVEEANYRGELLRNYSIVNKGYNSFPTNIPGTLYKKALKARKRLGEIISSIIS 245
           GILAIFGH+ + + + EL +NYSIV+KGYNSFP N+PGTLY+KAL+ARK+LG+I+S II 
Sbjct: 188 GILAIFGHL-DGHVKEELKKNYSIVDKGYNSFPINLPGTLYRKALQARKKLGKILSEIIR 247

Query: 246 E-KRKELVEKNLLGCLLKWRDENG-ESLSDEQIADNIIGVLFAAQDTTASVLTWVVKYLH 305
           E K K+ +EK LL C L  ++E G   L+++QIADNIIGVLFAAQDTTASVLTW++KYLH
Sbjct: 248 EMKEKKTLEKGLLSCFLNAKEEKGFLVLNEDQIADNIIGVLFAAQDTTASVLTWIIKYLH 307

Query: 306 DNQKVLEAVK------------------------------VVLESLRMASIISFTFREAV 365
           DN K+LE VK                              VVLE+LRMASIISF FREAV
Sbjct: 308 DNPKLLECVKAEQKVIWQSNEQENHGLTWTQTRKMPITSRVVLETLRMASIISFAFREAV 367

Query: 366 ADVEYNGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPSRFEVAPRPNTFMPFGGGVHAC 425
           ADVEY GYLIPKGWKVMPLFRNIHHNP++F DP KFDPSRFE AP+PNTFMPFG GVHAC
Sbjct: 368 ADVEYKGYLIPKGWKVMPLFRNIHHNPEFFPDPQKFDPSRFENAPKPNTFMPFGSGVHAC 427

Query: 426 PGNELAKLEILIMIHHLVTKFRWELAGERDGIQHGPFPVPMDGLPIRVWRLST 442
           PGNELAKLEILIM HHLVTKFRWE+ G   GIQ+GPFPVP+ GL  R W+ ++
Sbjct: 428 PGNELAKLEILIMTHHLVTKFRWEVVGSGSGIQYGPFPVPLGGLAARFWKTTS 475

[Close]

BLAST of Spg025447 vs. ExPASy Swiss-Prot
Match: Q0J185 (Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP707A7 PE=2 SV=1)

HSP 1 Score: 587.8 bits (1514), Expect = 1.0e-166
Identity = 286/437 (65.45%), Postives = 342/437 (78.26%), Query Frame = 0


Query: 34  AKKLPPGSMGWPLIGETFRLYSQHPSSFFLGKQKRYGDIFKTHILGCPCVMLATPEAARF 93
           A +LPPGSMGWP IGET +LYSQ P+ FF  KQKRYG+IFKTHILGCPCVMLA+PEAARF
Sbjct: 41  AVRLPPGSMGWPYIGETLQLYSQDPNVFFASKQKRYGEIFKTHILGCPCVMLASPEAARF 100

Query: 94  VLVTQAHLFKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPDIHAL 153
           VLVTQAHLFKPTYP+SKER+IGPSALFFHQ + HLRLRKL+Q  L PD +RALVPD+ A 
Sbjct: 101 VLVTQAHLFKPTYPRSKERMIGPSALFFHQGDYHLRLRKLVQGPLGPDALRALVPDVEAA 160

Query: 154 AVSTLPAWLNGHLINTFHQMKKFSFEVGILAIFGHVEEANYRGELLRNYSIVNKGYNSFP 213
             STL +W +G++ +TFH MK+ SF+VGI+ IFG   +   + EL +NY+IV KGYNSFP
Sbjct: 161 VRSTLASW-DGNVSSTFHAMKRLSFDVGIVTIFGGRLDERRKAELRQNYAIVEKGYNSFP 220

Query: 214 TNIPGTLYKKALKARKRLGEIISSIISEKR-KELVEKNLLGCLLKWR-DENGESLSDEQI 273
            + PGTLY KA++AR+RL  ++S I+ E+R +     +LLGCL++ R  ++G  L+DEQ+
Sbjct: 221 NSFPGTLYYKAIQARRRLHGVLSDIMRERRARGEPGSDLLGCLMQSRAGDDGALLTDEQV 280

Query: 274 ADNIIGVLFAAQDTTASVLTWVVKYLHDNQKVLEAV------------------------ 333
           ADNIIGVLFAAQDTTASVLTW+VKYLHD+ K+LEAV                        
Sbjct: 281 ADNIIGVLFAAQDTTASVLTWIVKYLHDHPKLLEAVRAEQAAIRAANDGGRLPLTWAQTR 340

Query: 334 ------KVVLESLRMASIISFTFREAVADVEYNGYLIPKGWKVMPLFRNIHHNPDYFVDP 393
                 KV+LESLRMASIISFTFREAVADVEY G+LIPKGWKVMPLFRNIHHNPDYF DP
Sbjct: 341 SMALTHKVILESLRMASIISFTFREAVADVEYKGFLIPKGWKVMPLFRNIHHNPDYFQDP 400

Query: 394 HKFDPSRFEVAPRPNTFMPFGGGVHACPGNELAKLEILIMIHHLVTKFRWELAGERDGIQ 439
            KFDPSRF+V+PRPNTFMPFG GVHACPGNELAKLE+L++IHHLVT +RWE+ G  D ++
Sbjct: 401 QKFDPSRFKVSPRPNTFMPFGNGVHACPGNELAKLEMLVLIHHLVTGYRWEIVGSSDEVE 460

[Close]

BLAST of Spg025447 vs. ExPASy Swiss-Prot
Match: A2Z212 (Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A7 PE=3 SV=1)

HSP 1 Score: 585.9 bits (1509), Expect = 3.9e-166
Identity = 286/440 (65.00%), Postives = 343/440 (77.95%), Query Frame = 0


Query: 34  AKKLPPGSMGWPLIGETFRLYSQHPSSFFLGKQKRYGDIFKTHILGCPCVMLATPEAARF 93
           A +LPPGSMGWP IGET +LYSQ P+ FF  KQKRYG+IFKTHILGCPCVMLA+PEAARF
Sbjct: 41  AVRLPPGSMGWPYIGETLQLYSQDPNVFFASKQKRYGEIFKTHILGCPCVMLASPEAARF 100

Query: 94  VLVTQAHLFKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPDIHAL 153
           VLVTQAHLFKPTYP+SKER+IGPSALFF+Q + HLRLRKL+Q  L PD +RALVPD+ A 
Sbjct: 101 VLVTQAHLFKPTYPRSKERMIGPSALFFNQGDYHLRLRKLVQGPLGPDALRALVPDVEAA 160

Query: 154 AVSTLPAWLNGHLINTFHQMKKFSFEVGILAIFGHVEEANYRGELLRNYSIVNKGYNSFP 213
             STL +W +G++ +TFH MK+ SF+VGI+ IFG   +   + EL +NY+IV KGYNSFP
Sbjct: 161 VRSTLASW-DGNVSSTFHAMKRLSFDVGIVTIFGGRLDERRKAELRQNYAIVEKGYNSFP 220

Query: 214 TNIPGTLYKKALKARKRLGEIISSIISEKR-KELVEKNLLGCLLKWR-DENGESLSDEQI 273
            + PGTLY KA++AR+RL  ++S I+ E+R +     +LLGCL++ R  ++G  L+DEQ+
Sbjct: 221 NSFPGTLYYKAIQARRRLHGVLSDIMRERRARGEPGSDLLGCLMQSRAGDDGALLTDEQV 280

Query: 274 ADNIIGVLFAAQDTTASVLTWVVKYLHDNQKVLEAV------------------------ 333
           ADNIIGVLFAAQDTTASVLTW+VKYLHD+ K+LEAV                        
Sbjct: 281 ADNIIGVLFAAQDTTASVLTWIVKYLHDHPKLLEAVRAEQAAIRAANDGGRLPLTWAQTR 340

Query: 334 ------KVVLESLRMASIISFTFREAVADVEYNGYLIPKGWKVMPLFRNIHHNPDYFVDP 393
                 KV+LESLRMASIISFTFREAVADVEY G+LIPKGWKVMPLFRNIHHNPDYF DP
Sbjct: 341 SMALTHKVILESLRMASIISFTFREAVADVEYKGFLIPKGWKVMPLFRNIHHNPDYFQDP 400

Query: 394 HKFDPSRFEVAPRPNTFMPFGGGVHACPGNELAKLEILIMIHHLVTKFRWELAGERDGIQ 442
            KFDPSRF+V+PRPNTFMPFG GVHACPGNELAKLE+L++IHHLVT +RWE+ G  D ++
Sbjct: 401 QKFDPSRFKVSPRPNTFMPFGNGVHACPGNELAKLEMLVLIHHLVTGYRWEIVGSSDEVE 460

[Close]

BLAST of Spg025447 vs. ExPASy Swiss-Prot
Match: Q9LJK2 (Abscisic acid 8'-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=CYP707A4 PE=2 SV=2)

HSP 1 Score: 577.8 bits (1488), Expect = 1.1e-163
Identity = 286/459 (62.31%), Postives = 351/459 (76.47%), Query Frame = 0


Query: 6   FLLISIFPLALLLLLPLIFFHVQKNKNIAKKLPPGSMGWPLIGETFRLYSQHPSSFFLGK 65
           FL++ I  L LLL+  ++    +K KN   KLPPGSMGWP +GET +LYSQ+P+ FF  K
Sbjct: 6   FLVVPILILCLLLVRVIV---SKKKKNSRGKLPPGSMGWPYLGETLQLYSQNPNVFFTSK 65

Query: 66  QKRYGDIFKTHILGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFHQSE 125
           QKRYG+IFKT ILG PCVMLA+PEAARFVLVT AH+FKPTYP+SKE+LIGPSALFFHQ +
Sbjct: 66  QKRYGEIFKTRILGYPCVMLASPEAARFVLVTHAHMFKPTYPRSKEKLIGPSALFFHQGD 125

Query: 126 THLRLRKLIQSSLSPDPVRALVPDIHALAVSTLPAWLNGHLINTFHQMKKFSFEVGILAI 185
            H  +RKL+QSS  P+ +R L+PDI  +A+S+L +W N  +++T+ +MKKF+F+VGILAI
Sbjct: 126 YHSHIRKLVQSSFYPETIRKLIPDIEHIALSSLQSWANMPIVSTYQEMKKFAFDVGILAI 185

Query: 186 FGHVEEANYRGELLRNYSIVNKGYNSFPTNIPGTLYKKALKARKRLGEIISSIISEKR-K 245
           FGH+ E++Y+  L  NY+IV+KGYNSFP ++PGT Y KAL ARK+L  I+S II E+R K
Sbjct: 186 FGHL-ESSYKEILKHNYNIVDKGYNSFPMSLPGTSYHKALMARKQLKTIVSEIICERREK 245

Query: 246 ELVEKNLLGCLLKWRDENGESLSDEQIADNIIGVLFAAQDTTASVLTWVVKYLHDNQKVL 305
             ++ + LG LL +++E G  L+ EQIADNIIGVLFAAQDTTAS LTW++KYLHD+QK+L
Sbjct: 246 RALQTDFLGHLLNFKNEKGRVLTQEQIADNIIGVLFAAQDTTASCLTWILKYLHDDQKLL 305

Query: 306 EAV------------------------------KVVLESLRMASIISFTFREAVADVEYN 365
           EAV                              KV++ESLRMASIISFTFREAV DVEY 
Sbjct: 306 EAVKAEQKAIYEENSREKKPLTWRQTRNMPLTHKVIVESLRMASIISFTFREAVVDVEYK 365

Query: 366 GYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPSRFEVAPRPNTFMPFGGGVHACPGNELA 425
           GYLIPKGWKVMPLFRNIHHNP YF +P  FDPSRFEV P+PNTFMPFG GVHACPGNELA
Sbjct: 366 GYLIPKGWKVMPLFRNIHHNPKYFSNPEVFDPSRFEVNPKPNTFMPFGSGVHACPGNELA 425

Query: 426 KLEILIMIHHLVTKFRWELAGERDGIQHGPFPVPMDGLP 434
           KL+ILI +HHLV+ FRWE+ G   GIQ+ PFP+P +GLP
Sbjct: 426 KLQILIFLHHLVSNFRWEVKGGEKGIQYSPFPIPQNGLP 460

[Close]

BLAST of Spg025447 vs. ExPASy Swiss-Prot
Match: Q09J78 (Abscisic acid 8'-hydroxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A6 PE=2 SV=1)

HSP 1 Score: 574.7 bits (1480), Expect = 8.9e-163
Identity = 277/446 (62.11%), Postives = 340/446 (76.23%), Query Frame = 0


Query: 28  QKNKNIAKKLPPGSMGWPLIGETFRLYSQHPSSFFLGKQKRYGDIFKTHILGCPCVMLAT 87
           QK K +  +LPPGSMGWP +GET +LYSQ P+ FF  KQKRYG+IFKT++LGCPCVMLA+
Sbjct: 44  QKKKKL--RLPPGSMGWPYVGETLQLYSQDPNVFFASKQKRYGEIFKTNLLGCPCVMLAS 103

Query: 88  PEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALV 147
           PEAARFVLV+QA LFKPTYP SKER+IGPSALFFHQ E HLRLR+L+Q++L+PD +RALV
Sbjct: 104 PEAARFVLVSQARLFKPTYPPSKERMIGPSALFFHQGEYHLRLRRLVQAALAPDSLRALV 163

Query: 148 PDIHALAVSTLPAWLNGHLINTFHQMKKFSFEVGILAIFGHVEEANYRGELLRNYSIVNK 207
           PD+ A   +TL AW  GH+ +TFH MKK SF+VG++ IFG      +R EL  NYS+V +
Sbjct: 164 PDVDAAVAATLAAWSGGHVASTFHAMKKLSFDVGVVTIFGGRLGRRHREELRTNYSVVER 223

Query: 208 GYNSFPTNIPGTLYKKALKARKRLGEIISSIISEKR-KELVEKNLLGCLLKWRDENGES- 267
           GYN FP   PGTLY KA++ARKRL  I+S I++E+R +     +LLG L++ RD+     
Sbjct: 224 GYNCFPNRFPGTLYHKAIQARKRLRAILSEIVAERRARGGGGDDLLGGLMRSRDDGTAGA 283

Query: 268 ---LSDEQIADNIIGVLFAAQDTTASVLTWVVKYLHDNQKVLEAVK-------------- 327
              L+D+QIADN++GVLFAAQDTTASVLTW++KYLHD+ K+LEAVK              
Sbjct: 284 VALLTDDQIADNVVGVLFAAQDTTASVLTWILKYLHDSPKLLEAVKAEQMAIYVANEGGK 343

Query: 328 ----------------VVLESLRMASIISFTFREAVADVEYNGYLIPKGWKVMPLFRNIH 387
                           V+LESLRMASIISFTFREAVADVEY G+LIPKGWKVMPLFRNIH
Sbjct: 344 RPLTWTQTRSMTLTHQVILESLRMASIISFTFREAVADVEYKGFLIPKGWKVMPLFRNIH 403

Query: 388 HNPDYFVDPHKFDPSRFEVAPRPNTFMPFGGGVHACPGNELAKLEILIMIHHLVTKFRWE 439
           HNPDYF DP KFDPSRF+VAPRP+TF+PFG GVHACPGNELAKLE+L+++H LVT +RWE
Sbjct: 404 HNPDYFQDPQKFDPSRFKVAPRPSTFLPFGSGVHACPGNELAKLEMLVLVHRLVTAYRWE 463

[Close]

BLAST of Spg025447 vs. ExPASy TrEMBL
Match: A0A6J1HCL5 (abscisic acid 8'-hydroxylase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111462760 PE=3 SV=1)

HSP 1 Score: 741.5 bits (1913), Expect = 2.0e-210
Identity = 372/473 (78.65%), Postives = 396/473 (83.72%), Query Frame = 0


Query: 2   MPQNFLLISIFPLALLLLLPLIFFHVQKNKNIA-KKLPPGSMGWPLIGETFRLYSQHPSS 61
           M   F+LISIF      LL   F+ + KN  I  KKLPPGSMGWPLIGETF  YSQ   +
Sbjct: 1   MAATFVLISIF----FPLLLCFFWKIHKNNRIPNKKLPPGSMGWPLIGETFHFYSQ-DLT 60

Query: 62  FFLGKQKRYGDIFKTHILGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALF 121
           FF  KQKRYG+IFKTHI GCPCVML TPEAARFVLVTQAHLFKPTYPQSKERLIGPSALF
Sbjct: 61  FFFRKQKRYGEIFKTHIHGCPCVMLTTPEAARFVLVTQAHLFKPTYPQSKERLIGPSALF 120

Query: 122 FHQSETHLRLRKLIQSSLSPDPVRALVPDIHALAVSTLPAWLNGHLINTFHQMKKFSFEV 181
           FHQSETHLRLRKLIQSSLSPDPVR LVP IHALA+STLP+WLNGHLINT HQMKKFSFEV
Sbjct: 121 FHQSETHLRLRKLIQSSLSPDPVRTLVPHIHALAISTLPSWLNGHLINTLHQMKKFSFEV 180

Query: 182 GILAIFGHVEEANYRGELLRNYSIVNKGYNSFPTNIPGTLYKKALKARKRLGEIISSIIS 241
           GIL IFGH+E ANYR ELL+NYSIVNKGYNSFPTN PG++YKKALKAR RLGEII+ IIS
Sbjct: 181 GILVIFGHLENANYRDELLKNYSIVNKGYNSFPTNFPGSIYKKALKARNRLGEIINCIIS 240

Query: 242 EK---RKELVEKNLLGCLLKWRDENGESLSDEQIADNIIGVLFAAQDTTASVLTWVVKYL 301
           EK   RKEL EKNLL CLLKWRDE GESLSD+QIADNIIGVLFAAQDTTASVLTWVVKYL
Sbjct: 241 EKREQRKELEEKNLLACLLKWRDEKGESLSDDQIADNIIGVLFAAQDTTASVLTWVVKYL 300

Query: 302 HDNQKVLEAV------------------------------KVVLESLRMASIISFTFREA 361
           HDN K+LEAV                              KVVLESLRMA++ISF+FREA
Sbjct: 301 HDNDKILEAVKAEQRAIQEAINEGSRAMSWADTRNMPFTNKVVLESLRMATVISFSFREA 360

Query: 362 VADVEYNGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPSRFEVAPRPNTFMPFGGGVHA 421
           V DVEYNGYLIPKGWKVMPLFRNIHHNPDYF+ PHKFDPSRFEVAPRPNTF+PFG GVHA
Sbjct: 361 VTDVEYNGYLIPKGWKVMPLFRNIHHNPDYFLHPHKFDPSRFEVAPRPNTFIPFGSGVHA 420

Query: 422 CPGNELAKLEILIMIHHLVTKFRWELAGERDGIQHGPFPVPMDGLPIRVWRLS 441
           CPGN+LAKLEILIMIHHLVTKFRWELAG++DGI+HGPFPVP DGLPIR+WRLS
Sbjct: 421 CPGNDLAKLEILIMIHHLVTKFRWELAGQKDGIEHGPFPVPRDGLPIRLWRLS 468

[Close]

BLAST of Spg025447 vs. ExPASy TrEMBL
Match: A0A6J1CQS3 (abscisic acid 8'-hydroxylase 4-like OS=Momordica charantia OX=3673 GN=LOC111013891 PE=3 SV=1)

HSP 1 Score: 738.4 bits (1905), Expect = 1.7e-209
Identity = 372/475 (78.32%), Postives = 399/475 (84.00%), Query Frame = 0


Query: 2   MPQNFLLISIF--PLALLLLLPLIFFHVQKNKNIAKKLPPGSMGWPLIGETFRLYSQHPS 61
           M + F+L SIF   L LLLLLPLIFF  + NK   KKLPPGSMGWPLIGETF LYS+ P+
Sbjct: 1   MGEIFVLFSIFLPTLLLLLLLPLIFFKKENNK--GKKLPPGSMGWPLIGETFHLYSRDPT 60

Query: 62  SFFLGKQKRYGDIFKTHILGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSAL 121
            FFL KQKRYG+IFKTHI GCPCVMLATPEAARFVLVTQAHLFKPTYP  KERLIGPSAL
Sbjct: 61  IFFLDKQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHLFKPTYPHGKERLIGPSAL 120

Query: 122 FFHQSETHLRLRKLIQSSLSPDPVRALVPDIHALAVSTLPAWLNGHLINTFHQMKKFSFE 181
           FFHQS+ HLRLRKL+QSSLSPDPVRALVP I ALA S L  WL+GH +NTF +MKKFSFE
Sbjct: 121 FFHQSDAHLRLRKLVQSSLSPDPVRALVPYIDALASSALAFWLHGHTVNTFEEMKKFSFE 180

Query: 182 VGILAIFGHVEEANYRGELLRNYSIVNKGYNSFPTNIPGTLYKKALKARKRLGEIISSII 241
           VGILAIFGH+E+ NYR EL++NYS+VN+GYNSF TNIPGT+YKKALKARKRLGEIIS II
Sbjct: 181 VGILAIFGHLEDDNYRDELMKNYSMVNRGYNSFLTNIPGTIYKKALKARKRLGEIISCII 240

Query: 242 SEKR----KELVEKNLLGCLLKWRDENGESLSDEQIADNIIGVLFAAQDTTASVLTWVVK 301
           SEKR    K+L EKNLLGCLLKWRDE GE+LSDEQIADN+IGVLFAAQDTTASVLTWV+K
Sbjct: 241 SEKRQNTTKKLAEKNLLGCLLKWRDEKGETLSDEQIADNLIGVLFAAQDTTASVLTWVLK 300

Query: 302 YLHDNQKVLEAV------------------------------KVVLESLRMASIISFTFR 361
           YLHDN+KVL+AV                              KVVLESLRMASII+FTFR
Sbjct: 301 YLHDNRKVLQAVKAEQKAIEEAINGGSRPMSWSHTRNMPFTNKVVLESLRMASIITFTFR 360

Query: 362 EAVADVEYNGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPSRFEVAPRPNTFMPFGGGV 421
           EAVADVEYNGYLIPKGWKVMPLF NIHHN +YFVDPHKFDPSRFEVAPRPNTFMPFG GV
Sbjct: 361 EAVADVEYNGYLIPKGWKVMPLFSNIHHNSEYFVDPHKFDPSRFEVAPRPNTFMPFGSGV 420

Query: 422 HACPGNELAKLEILIMIHHLVTKFRWELAGERDGIQHGPFPVPMDGLPIRVWRLS 441
           HACPGNELAKLEILIMIHHLVTKFRWE AG  DGI HGPFPVP+DGLPIRVWRLS
Sbjct: 421 HACPGNELAKLEILIMIHHLVTKFRWEFAGRGDGIHHGPFPVPLDGLPIRVWRLS 473

[Close]

BLAST of Spg025447 vs. ExPASy TrEMBL
Match: A0A5A7V0Q4 (Abscisic acid 8'-hydroxylase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00370 PE=3 SV=1)

HSP 1 Score: 731.5 bits (1887), Expect = 2.1e-207
Identity = 361/434 (83.18%), Postives = 379/434 (87.33%), Query Frame = 0


Query: 42  MGWPLIGETFRLYSQHPSSFFLGKQKRYGDIFKTHILGCPCVMLATPEAARFVLVTQAHL 101
           MGWPLIGETF  YSQHP+SFFLGKQKRYG+IFKTHI GCPCVMLATPEAARFVLVTQAHL
Sbjct: 1   MGWPLIGETFHFYSQHPTSFFLGKQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHL 60

Query: 102 FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPDIHALAVSTLPAW 161
           FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVP IHALA+S LP+ 
Sbjct: 61  FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPHIHALAISALPSS 120

Query: 162 LNGHLINTFHQMKKFSFEVGILAIFGHVEEANYRGELLRNYSIVNKGYNSFPTNIPGTLY 221
           L+GH+I+T+HQMKKFSFEVGILAIFGH+E+ NYR EL  NYSIVNKGYNSFPTNIPGTLY
Sbjct: 121 LSGHVIDTYHQMKKFSFEVGILAIFGHLEDTNYREELRTNYSIVNKGYNSFPTNIPGTLY 180

Query: 222 KKALKARKRLGEIISSIISEKR----KELVEKNLLGCLLKWRDENGESLSDEQIADNIIG 281
           KKA+KARKRL EIIS II EK+    K L EKNLL CL+KWRDE GESLSDEQIADNIIG
Sbjct: 181 KKAIKARKRLEEIISCIILEKKEKRSKVLGEKNLLSCLVKWRDEKGESLSDEQIADNIIG 240

Query: 282 VLFAAQDTTASVLTWVVKYLHDNQKVLEAV------------------------------ 341
           VLFAAQDTTASVLTWVVKYLHDN KVLEAV                              
Sbjct: 241 VLFAAQDTTASVLTWVVKYLHDNPKVLEAVKVEQKVIEEDINEGSRPMRWSHTRNMPFTN 300

Query: 342 KVVLESLRMASIISFTFREAVADVEYNGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPS 401
           KVVLESLRMASIISFTFREAVADVEY GYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPS
Sbjct: 301 KVVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPS 360

Query: 402 RFEVAPRPNTFMPFGGGVHACPGNELAKLEILIMIHHLVTKFRWELAGERDGIQHGPFPV 442
           RFEVAPRPNTFMPFG GVH+CPGNELAKLEI IMIHHLVTKFRWELAG+RDGI+HGPFPV
Sbjct: 361 RFEVAPRPNTFMPFGSGVHSCPGNELAKLEIFIMIHHLVTKFRWELAGQRDGIEHGPFPV 420

[Close]

BLAST of Spg025447 vs. ExPASy TrEMBL
Match: A0A0A0LKV6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G357280 PE=3 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 8.0e-207
Identity = 361/436 (82.80%), Postives = 380/436 (87.16%), Query Frame = 0


Query: 42  MGWPLIGETFRLYSQHPSSFFLGKQKRYGDIFKTHILGCPCVMLATPEAARFVLVTQAHL 101
           MGWPLIGETF  YSQHP+SFFLGKQKRYG+IFKTHI GCPCVMLATPEAARF+LVTQAHL
Sbjct: 1   MGWPLIGETFHFYSQHPTSFFLGKQKRYGEIFKTHIHGCPCVMLATPEAARFILVTQAHL 60

Query: 102 FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPDIHALAVSTLPAW 161
           FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVP IHALA+S LP+ 
Sbjct: 61  FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPHIHALAISALPSS 120

Query: 162 L-NGHLINTFHQMKKFSFEVGILAIFGHVEEANYRGELLRNYSIVNKGYNSFPTNIPGTL 221
           L NGHLI+T+HQMKKFSFEVGILAIFGH+E  NYR EL  NYSIVNKGYNSFPTNIPGTL
Sbjct: 121 LNNGHLIDTYHQMKKFSFEVGILAIFGHLENTNYREELRTNYSIVNKGYNSFPTNIPGTL 180

Query: 222 YKKALKARKRLGEIISSIISEKRKE-----LVEKNLLGCLLKWRDENGESLSDEQIADNI 281
           YKKA+KARKRL EIIS IISEK+++     L EKNLL CL+KWRDE GESLSDEQIADNI
Sbjct: 181 YKKAIKARKRLEEIISCIISEKKEKRSEVVLGEKNLLSCLVKWRDEKGESLSDEQIADNI 240

Query: 282 IGVLFAAQDTTASVLTWVVKYLHDNQKVLEAV---------------------------- 341
           IGVLFAAQDTTASVLTWVVKYLHDN K+LEAV                            
Sbjct: 241 IGVLFAAQDTTASVLTWVVKYLHDNPKILEAVKVEQKVIEEDINEGSGPMRWAHTRTMPF 300

Query: 342 --KVVLESLRMASIISFTFREAVADVEYNGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFD 401
             KVVLESLR+ASIISFTFREAVADVEY GYLIPKGWKVMPLFRNIHHNPDYFVDPHKFD
Sbjct: 301 TNKVVLESLRLASIISFTFREAVADVEYKGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFD 360

Query: 402 PSRFEVAPRPNTFMPFGGGVHACPGNELAKLEILIMIHHLVTKFRWELAGERDGIQHGPF 442
           PSRFEVAPRPNTFMPFG GVHACPGNELAKLEILIMIHHLVT FRWELAG+RDGI+HGPF
Sbjct: 361 PSRFEVAPRPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTNFRWELAGQRDGIEHGPF 420

[Close]

BLAST of Spg025447 vs. ExPASy TrEMBL
Match: A0A6J1K9Y7 (abscisic acid 8'-hydroxylase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111492434 PE=3 SV=1)

HSP 1 Score: 714.1 bits (1842), Expect = 3.5e-202
Identity = 350/432 (81.02%), Postives = 371/432 (85.88%), Query Frame = 0


Query: 42  MGWPLIGETFRLYSQHPSSFFLGKQKRYGDIFKTHILGCPCVMLATPEAARFVLVTQAHL 101
           MGWPLIGETF  YSQ   +FF  KQKRYG+IFKTHI GCPCVML TPEAARFVLVTQAHL
Sbjct: 1   MGWPLIGETFHFYSQ-DLTFFFRKQKRYGEIFKTHIHGCPCVMLTTPEAARFVLVTQAHL 60

Query: 102 FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPDIHALAVSTLPAW 161
           FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVR LVP IHALA+STLP+W
Sbjct: 61  FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRTLVPHIHALAISTLPSW 120

Query: 162 LNGHLINTFHQMKKFSFEVGILAIFGHVEEANYRGELLRNYSIVNKGYNSFPTNIPGTLY 221
           LNGHLINT HQMKKFSFEVGIL IFGH+E ANYR ELL+NYSIVNKGYNSFPTN PG++Y
Sbjct: 121 LNGHLINTLHQMKKFSFEVGILVIFGHLENANYRDELLKNYSIVNKGYNSFPTNFPGSIY 180

Query: 222 KKALKARKRLGEIISSIISEK---RKELVEKNLLGCLLKWRDENGESLSDEQIADNIIGV 281
           KKALKAR RLGEII+ IISEK   RKEL EKNLL CLLKWRD  GESLSD+QIADNIIGV
Sbjct: 181 KKALKARNRLGEIINCIISEKREQRKELEEKNLLACLLKWRDAKGESLSDDQIADNIIGV 240

Query: 282 LFAAQDTTASVLTWVVKYLHDNQKVLEAV------------------------------K 341
           LFAAQDTTASVLTWVVKYLHDN K+LEAV                              K
Sbjct: 241 LFAAQDTTASVLTWVVKYLHDNDKILEAVKVEQRAIEEAINEGSRAMSWADTRNMPFTNK 300

Query: 342 VVLESLRMASIISFTFREAVADVEYNGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPSR 401
           VVLESLRMA++ISF+FREAV DVEYNGYLIPKGWKVMPLFRNIHHNPDYF+ PHKFDPSR
Sbjct: 301 VVLESLRMATVISFSFREAVTDVEYNGYLIPKGWKVMPLFRNIHHNPDYFLHPHKFDPSR 360

Query: 402 FEVAPRPNTFMPFGGGVHACPGNELAKLEILIMIHHLVTKFRWELAGERDGIQHGPFPVP 441
           FEVAPRPNTF+PFG GVHACPGN+LAKLEILIMIHHLVTKFRWELAG++DGI+HGPFPVP
Sbjct: 361 FEVAPRPNTFIPFGSGVHACPGNDLAKLEILIMIHHLVTKFRWELAGQKDGIEHGPFPVP 420

[Close]

BLAST of Spg025447 vs. TAIR 10
Match: AT3G19270.1 (cytochrome P450, family 707, subfamily A, polypeptide 4 )

HSP 1 Score: 577.8 bits (1488), Expect = 7.5e-165
Identity = 286/459 (62.31%), Postives = 351/459 (76.47%), Query Frame = 0


Query: 6   FLLISIFPLALLLLLPLIFFHVQKNKNIAKKLPPGSMGWPLIGETFRLYSQHPSSFFLGK 65
           FL++ I  L LLL+  ++    +K KN   KLPPGSMGWP +GET +LYSQ+P+ FF  K
Sbjct: 6   FLVVPILILCLLLVRVIV---SKKKKNSRGKLPPGSMGWPYLGETLQLYSQNPNVFFTSK 65

Query: 66  QKRYGDIFKTHILGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFHQSE 125
           QKRYG+IFKT ILG PCVMLA+PEAARFVLVT AH+FKPTYP+SKE+LIGPSALFFHQ +
Sbjct: 66  QKRYGEIFKTRILGYPCVMLASPEAARFVLVTHAHMFKPTYPRSKEKLIGPSALFFHQGD 125

Query: 126 THLRLRKLIQSSLSPDPVRALVPDIHALAVSTLPAWLNGHLINTFHQMKKFSFEVGILAI 185
            H  +RKL+QSS  P+ +R L+PDI  +A+S+L +W N  +++T+ +MKKF+F+VGILAI
Sbjct: 126 YHSHIRKLVQSSFYPETIRKLIPDIEHIALSSLQSWANMPIVSTYQEMKKFAFDVGILAI 185

Query: 186 FGHVEEANYRGELLRNYSIVNKGYNSFPTNIPGTLYKKALKARKRLGEIISSIISEKR-K 245
           FGH+ E++Y+  L  NY+IV+KGYNSFP ++PGT Y KAL ARK+L  I+S II E+R K
Sbjct: 186 FGHL-ESSYKEILKHNYNIVDKGYNSFPMSLPGTSYHKALMARKQLKTIVSEIICERREK 245

Query: 246 ELVEKNLLGCLLKWRDENGESLSDEQIADNIIGVLFAAQDTTASVLTWVVKYLHDNQKVL 305
             ++ + LG LL +++E G  L+ EQIADNIIGVLFAAQDTTAS LTW++KYLHD+QK+L
Sbjct: 246 RALQTDFLGHLLNFKNEKGRVLTQEQIADNIIGVLFAAQDTTASCLTWILKYLHDDQKLL 305

Query: 306 EAV------------------------------KVVLESLRMASIISFTFREAVADVEYN 365
           EAV                              KV++ESLRMASIISFTFREAV DVEY 
Sbjct: 306 EAVKAEQKAIYEENSREKKPLTWRQTRNMPLTHKVIVESLRMASIISFTFREAVVDVEYK 365

Query: 366 GYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPSRFEVAPRPNTFMPFGGGVHACPGNELA 425
           GYLIPKGWKVMPLFRNIHHNP YF +P  FDPSRFEV P+PNTFMPFG GVHACPGNELA
Sbjct: 366 GYLIPKGWKVMPLFRNIHHNPKYFSNPEVFDPSRFEVNPKPNTFMPFGSGVHACPGNELA 425

Query: 426 KLEILIMIHHLVTKFRWELAGERDGIQHGPFPVPMDGLP 434
           KL+ILI +HHLV+ FRWE+ G   GIQ+ PFP+P +GLP
Sbjct: 426 KLQILIFLHHLVSNFRWEVKGGEKGIQYSPFPIPQNGLP 460

[Close]

BLAST of Spg025447 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 515.8 bits (1327), Expect = 3.5e-146
Identity = 251/462 (54.33%), Postives = 334/462 (72.29%), Query Frame = 0


Query: 7   LLISIFPLALLLLLPLIFFHVQKNKNIAKKLPPGSMGWPLIGETFRLYSQHPSSFFLGKQ 66
           L +++   AL L L      V+++ +    LPPG+MG+P +GETF+LYSQ P+ FF  KQ
Sbjct: 6   LFLTLSAAALFLCLLRFIAGVRRSSSTKLPLPPGTMGYPYVGETFQLYSQDPNVFFAAKQ 65

Query: 67  KRYGDIFKTHILGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFHQSET 126
           +RYG +FKTH+LGCPCVM+++PEAA+FVLVT++HLFKPT+P SKER++G  A+FFHQ + 
Sbjct: 66  RRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDY 125

Query: 127 HLRLRKLIQSSLSPDPVRALVPDIHALAVSTLPAWLNGHLINTFHQMKKFSFEVGILAIF 186
           H +LRKL+  +  PD +R +VP I ++A  +L +W +G  +NT+ +MK ++F V +++I 
Sbjct: 126 HSKLRKLVLRAFMPDAIRNMVPHIESIAQESLNSW-DGTQLNTYQEMKTYTFNVALISIL 185

Query: 187 GHVEEANYRGELLRNYSIVNKGYNSFPTNIPGTLYKKALKARKRLGEIISSIISEKRKE- 246
           G  +E  YR +L R Y I+ KGYNS P N+PGTL+ KA+KARK L +I+++I+S++R+  
Sbjct: 186 GK-DEVYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKRRQNP 245

Query: 247 LVEKNLLGCLLKWRDENGESLSDEQIADNIIGVLFAAQDTTASVLTWVVKYLHDNQKVLE 306
               +LLG  +    E+   L+DEQIADNIIGV+FAA+DTTASVLTW++KYL DN  VLE
Sbjct: 246 SSHTDLLGSFM----EDKAGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVLE 305

Query: 307 AV-----------------------------KVVLESLRMASIISFTFREAVADVEYNGY 366
           AV                             +V+ E+LR A+I+SFTFREAV DVEY GY
Sbjct: 306 AVTEEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDVEYEGY 365

Query: 367 LIPKGWKVMPLFRNIHHNPDYFVDPHKFDPSRFEVAPRPNTFMPFGGGVHACPGNELAKL 426
           LIPKGWKV+PLFRNIHHN D F DP KFDPSRFEVAP+PNTFMPFG G+H+CPGNELAKL
Sbjct: 366 LIPKGWKVLPLFRNIHHNADIFSDPGKFDPSRFEVAPKPNTFMPFGSGIHSCPGNELAKL 425

Query: 427 EILIMIHHLVTKFRWELAGERDGIQHGPFPVPMDGLPIRVWR 439
           EI ++IHHL TK+RW + G  DGIQ+GPF +P +GLPI + R
Sbjct: 426 EISVLIHHLTTKYRWSIVGPSDGIQYGPFALPQNGLPIALER 461

[Close]

BLAST of Spg025447 vs. TAIR 10
Match: AT4G19230.1 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 512.3 bits (1318), Expect = 3.9e-145
Identity = 250/458 (54.59%), Postives = 331/458 (72.27%), Query Frame = 0


Query: 7   LLISIFPLALLLLLPLIFFHVQKNKNIAKKLPPGSMGWPLIGETFRLYSQHPSSFFLGKQ 66
           L +++F  +L L         ++  +    LPPG+MGWP +GETF+LYSQ P+ FF  KQ
Sbjct: 6   LFLTLFAGSLFLYFLRCLISQRRFGSSKLPLPPGTMGWPYVGETFQLYSQDPNVFFQSKQ 65

Query: 67  KRYGDIFKTHILGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFHQSET 126
           KRYG +FKTH+LGCPCVM+++PEAA+FVLVT++HLFKPT+P SKER++G  A+FFHQ + 
Sbjct: 66  KRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDY 125

Query: 127 HLRLRKLIQSSLSPDPVRALVPDIHALAVSTLPAWLNGHLINTFHQMKKFSFEVGILAIF 186
           H +LRKL+  +  P+ +R +VPDI ++A  +L +W  G +INT+ +MK ++F V +L+IF
Sbjct: 126 HAKLRKLVLRAFMPESIRNMVPDIESIAQDSLRSW-EGTMINTYQEMKTYTFNVALLSIF 185

Query: 187 GHVEEANYRGELLRNYSIVNKGYNSFPTNIPGTLYKKALKARKRLGEIISSIISEKRKEL 246
           G  +E  YR +L R Y I+ KGYNS P N+PGTL+ K++KARK L +I++ I+SE+R+  
Sbjct: 186 GK-DEVLYREDLKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSERRQNG 245

Query: 247 VEKN-LLGCLLKWRDENGESLSDEQIADNIIGVLFAAQDTTASVLTWVVKYLHDNQKVLE 306
              N LLG  +  ++E    L+DEQIADNIIGV+FAA+DTTASV++W++KYL +N  VLE
Sbjct: 246 SSHNDLLGSFMGDKEE----LTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLE 305

Query: 307 AV-----------------------------KVVLESLRMASIISFTFREAVADVEYNGY 366
           AV                             +V+ E+LR+ASI+SFTFREAV DVEY GY
Sbjct: 306 AVTEEQMAIRKDKEEGESLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDVEYEGY 365

Query: 367 LIPKGWKVMPLFRNIHHNPDYFVDPHKFDPSRFEVAPRPNTFMPFGGGVHACPGNELAKL 426
           LIPKGWKV+PLFRNIHH+ D F +P KFDPSRFEVAP+PNTFMPFG G H+CPGNELAKL
Sbjct: 366 LIPKGWKVLPLFRNIHHSADIFSNPGKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAKL 425

Query: 427 EILIMIHHLVTKFRWELAGERDGIQHGPFPVPMDGLPI 435
           E+ IMIHHL TK+ W + G  DGIQ+GPF +P +GLPI
Sbjct: 426 EMSIMIHHLTTKYSWSIVGASDGIQYGPFALPQNGLPI 457

[Close]

BLAST of Spg025447 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 502.3 bits (1292), Expect = 4.0e-142
Identity = 241/432 (55.79%), Postives = 309/432 (71.53%), Query Frame = 0


Query: 36  KLPPGSMGWPLIGETFRLYSQHPSSFFLGKQKRYGDIFKTHILGCPCVMLATPEAARFVL 95
           +LPPGSMG P IGET RLY+++P+SFF  +Q +YGDIFKTHILGCPCVM+++PEAAR VL
Sbjct: 48  RLPPGSMGLPYIGETLRLYTENPNSFFATRQNKYGDIFKTHILGCPCVMISSPEAARMVL 107

Query: 96  VTQAHLFKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPDIHALAV 155
           V++AHLFKPTYP SKER+IGP ALFFHQ   H  L++L+QSS  P  +R  V  I  L +
Sbjct: 108 VSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLKRLVQSSFMPSALRPTVSHIELLVL 167

Query: 156 STLPAWLNGHLINTFHQMKKFSFEVGILAIFGHVEEANYRGELLRNYSIVNKGYNSFPTN 215
            TL +W +   INT   MK+++F+V I++ FG  EE      +   Y  + +GYNS P +
Sbjct: 168 QTLSSWTSQKSINTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSMPLD 227

Query: 216 IPGTLYKKALKARKRLGEIISSIISEKRKE-LVEKNLLGCLLKWRDENGESLSDEQIADN 275
           +PGTL+ K++KAR  L E +  +I ++R+    E  LLG LL  +D+    LSD QIADN
Sbjct: 228 LPGTLFHKSMKARIELSEELRKVIEKRRENGREEGGLLGVLLGAKDQKRNGLSDSQIADN 287

Query: 276 IIGVLFAAQDTTASVLTWVVKYLHDNQKVLEAV--------------------------- 335
           IIGV+FAA DTTASVLTW++KYLHD+  +L+ V                           
Sbjct: 288 IIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMP 347

Query: 336 ---KVVLESLRMASIISFTFREAVADVEYNGYLIPKGWKVMPLFRNIHHNPDYFVDPHKF 395
              +V+ E+LR AS++SFTFREAV DVEY+GYLIPKGWKV+PLFR IHH+ ++F DP KF
Sbjct: 348 LTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKF 407

Query: 396 DPSRFEVAPRPNTFMPFGGGVHACPGNELAKLEILIMIHHLVTKFRWELAGERDGIQHGP 437
           DPSRFEVAP+P T+MPFG GVH+CPG+ELAKLE+LI++HHL T FRWE+ G+ +GIQ+GP
Sbjct: 408 DPSRFEVAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDEEGIQYGP 467

[Close]

BLAST of Spg025447 vs. TAIR 10
Match: AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 502.3 bits (1292), Expect = 4.0e-142
Identity = 241/432 (55.79%), Postives = 309/432 (71.53%), Query Frame = 0


Query: 36  KLPPGSMGWPLIGETFRLYSQHPSSFFLGKQKRYGDIFKTHILGCPCVMLATPEAARFVL 95
           +LPPGSMG P IGET RLY+++P+SFF  +Q +YGDIFKTHILGCPCVM+++PEAAR VL
Sbjct: 48  RLPPGSMGLPYIGETLRLYTENPNSFFATRQNKYGDIFKTHILGCPCVMISSPEAARMVL 107

Query: 96  VTQAHLFKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPDIHALAV 155
           V++AHLFKPTYP SKER+IGP ALFFHQ   H  L++L+QSS  P  +R  V  I  L +
Sbjct: 108 VSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLKRLVQSSFMPSALRPTVSHIELLVL 167

Query: 156 STLPAWLNGHLINTFHQMKKFSFEVGILAIFGHVEEANYRGELLRNYSIVNKGYNSFPTN 215
            TL +W +   INT   MK+++F+V I++ FG  EE      +   Y  + +GYNS P +
Sbjct: 168 QTLSSWTSQKSINTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSMPLD 227

Query: 216 IPGTLYKKALKARKRLGEIISSIISEKRKE-LVEKNLLGCLLKWRDENGESLSDEQIADN 275
           +PGTL+ K++KAR  L E +  +I ++R+    E  LLG LL  +D+    LSD QIADN
Sbjct: 228 LPGTLFHKSMKARIELSEELRKVIEKRRENGREEGGLLGVLLGAKDQKRNGLSDSQIADN 287

Query: 276 IIGVLFAAQDTTASVLTWVVKYLHDNQKVLEAV--------------------------- 335
           IIGV+FAA DTTASVLTW++KYLHD+  +L+ V                           
Sbjct: 288 IIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMP 347

Query: 336 ---KVVLESLRMASIISFTFREAVADVEYNGYLIPKGWKVMPLFRNIHHNPDYFVDPHKF 395
              +V+ E+LR AS++SFTFREAV DVEY+GYLIPKGWKV+PLFR IHH+ ++F DP KF
Sbjct: 348 LTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKF 407

Query: 396 DPSRFEVAPRPNTFMPFGGGVHACPGNELAKLEILIMIHHLVTKFRWELAGERDGIQHGP 437
           DPSRFEVAP+P T+MPFG GVH+CPG+ELAKLE+LI++HHL T FRWE+ G+ +GIQ+GP
Sbjct: 408 DPSRFEVAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDEEGIQYGP 467

The following BLAST results are available for this feature:

BLAST of Spg025447 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038884199.1	5.0e-219	82.77	abscisic acid 8'-hydroxylase CYP707A1-like [Benincasa hispida]	[more]
XP_023546528.1	2.5e-210	78.69	abscisic acid 8'-hydroxylase 3-like [Cucurbita pepo subsp. pepo]	[more]
XP_022962256.1	4.2e-210	78.65	abscisic acid 8'-hydroxylase 3-like [Cucurbita moschata]	[more]
XP_022144125.1	3.6e-209	78.32	abscisic acid 8'-hydroxylase 4-like [Momordica charantia]	[more]
KAA0060904.1	4.4e-207	83.18	abscisic acid 8'-hydroxylase 4-like [Cucumis melo var. makuwa]	[more]

BLAST of Spg025447 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A9QNE7	2.3e-171	63.64	Abscisic acid 8'-hydroxylase CYP707A1 OS=Solanum lycopersicum OX=4081 GN=CYP707A...	[more]
Q0J185	1.0e-166	65.45	Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP70...	[more]
A2Z212	3.9e-166	65.00	Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A...	[more]
Q9LJK2	1.1e-163	62.31	Abscisic acid 8'-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=CYP707A4 PE=2 ...	[more]
Q09J78	8.9e-163	62.11	Abscisic acid 8'-hydroxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A...	[more]

BLAST of Spg025447 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HCL5	2.0e-210	78.65	abscisic acid 8'-hydroxylase 3-like OS=Cucurbita moschata OX=3662 GN=LOC11146276...	[more]
A0A6J1CQS3	1.7e-209	78.32	abscisic acid 8'-hydroxylase 4-like OS=Momordica charantia OX=3673 GN=LOC1110138...	[more]
A0A5A7V0Q4	2.1e-207	83.18	Abscisic acid 8'-hydroxylase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6...	[more]
A0A0A0LKV6	8.0e-207	82.80	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G357280 PE=3 SV=1	[more]
A0A6J1K9Y7	3.5e-202	81.02	abscisic acid 8'-hydroxylase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111492434 ...	[more]

BLAST of Spg025447 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G19270.1	7.5e-165	62.31	cytochrome P450, family 707, subfamily A, polypeptide 4	[more]
AT5G45340.1	3.5e-146	54.33	cytochrome P450, family 707, subfamily A, polypeptide 3	[more]
AT4G19230.1	3.9e-145	54.59	cytochrome P450, family 707, subfamily A, polypeptide 1	[more]
AT2G29090.1	4.0e-142	55.79	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]
AT2G29090.2	4.0e-142	55.79	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 66..85 score: 31.32 coord: 347..371 score: 28.31 coord: 307..325 score: 23.01 coord: 378..388 score: 46.77 coord: 388..411 score: 28.29 coord: 270..287 score: 21.19
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 281..298 score: 33.35 coord: 379..388 score: 47.83 coord: 388..399 score: 39.37 coord: 308..319 score: 33.62
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 308..416 e-value: 1.3E-31 score: 109.8 coord: 38..307 e-value: 2.6E-25 score: 89.1
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 26..439 e-value: 5.2E-108 score: 363.7
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 35..437
None	No IPR available	PANTHER	PTHR24286:SF103	CYTOCHROME P450 FAMILY ABA 8'-HYDROXYLASE	coord: 309..437
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 309..437 coord: 21..308
None	No IPR available	PANTHER	PTHR24286:SF103	CYTOCHROME P450 FAMILY ABA 8'-HYDROXYLASE	coord: 21..308
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 381..390

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg025447.1	Spg025447.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016125	sterol metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0010295	(+)-abscisic acid 8'-hydroxylase activity
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen