Spg025428 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg025428 vs. NCBI nr
Match: XP_023522626.1 (probable carboxylesterase 18 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023547293.1 probable carboxylesterase 18 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 632.1 bits (1629), Expect = 2.7e-177
Identity = 298/336 (88.69%), Postives = 315/336 (93.75%), Query Frame = 0

Query: 1   MSEALSGLRLPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSF 60
           MS+ALS LRLPWKVKMMLTAIAVGTDICCRSD SVNR+L+NL+DFKSPPLKKPKNG+KSF
Sbjct: 1   MSDALSALRLPWKVKMMLTAIAVGTDICCRSDVSVNRFLANLMDFKSPPLKKPKNGVKSF 60

Query: 61  DVTIDASRNLWFRLYTPTSDSSSGSLPLIVYFHGGGFVYMAPDSKLLDDFCQRLAREISA 120
           DVT+DASRNLWFRLY PTS+S+S SLPLIVYFHGGGFVYMAPDSKLLDDFCQ LAREI A
Sbjct: 61  DVTVDASRNLWFRLYAPTSESTSESLPLIVYFHGGGFVYMAPDSKLLDDFCQSLAREIHA 120

Query: 121 VVISVNYRLAPENRYSCQYEDGFDVLKFIDGDGSPFEGLPANVDLKRCFLAGDSAGGNIA 180
           VVISVNYRLAPE+R  CQYEDGFDVLKFIDGDGS FEGLPANVDLKRCF+AGDSAGGNIA
Sbjct: 121 VVISVNYRLAPEHRCPCQYEDGFDVLKFIDGDGSSFEGLPANVDLKRCFVAGDSAGGNIA 180

Query: 181 HHTILKSAEHEFRELEIIGVIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYWKAFLPE 240
           HH ILKS +HEFRELEIIG+IAIQPFFGGEERTES+  LIKAPLST+DRTDWYWKAFLPE
Sbjct: 181 HHMILKSGDHEFRELEIIGLIAIQPFFGGEERTESDIRLIKAPLSTIDRTDWYWKAFLPE 240

Query: 241 GSDRDHPAVNIFGPNSVDISDVRFPAIKVFVGGLDPLIDWQKKYYEGLKKSGKEAYLTEY 300
           G DRDHP+VNIFGP SVDIS VRFPAIKV VGGLDPLIDWQK+YYEGLKKSGKEAYLTEY
Sbjct: 241 GFDRDHPSVNIFGPKSVDISKVRFPAIKVLVGGLDPLIDWQKRYYEGLKKSGKEAYLTEY 300

Query: 301 PNAFHSFYGFPELPEWSLFIKDAADFVRTQCSKKSS 337
           PNAFHSFYGFPELPEWS FIKD ADF+  QCSKKSS
Sbjct: 301 PNAFHSFYGFPELPEWSEFIKDVADFIAAQCSKKSS 336

BLAST of Spg025428 vs. NCBI nr
Match: XP_038885745.1 (probable carboxylesterase 18 [Benincasa hispida])

HSP 1 Score: 623.2 bits (1606), Expect = 1.3e-174
Identity = 294/336 (87.50%), Postives = 312/336 (92.86%), Query Frame = 0

Query: 1   MSEALSGLRLPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSF 60
           MS+ALS LRLPWKVKMML AIAVGTDICCRSD SVNR+LSNLLDFKSPPLKKPKNGLKSF
Sbjct: 1   MSDALSTLRLPWKVKMMLIAIAVGTDICCRSDVSVNRFLSNLLDFKSPPLKKPKNGLKSF 60

Query: 61  DVTIDASRNLWFRLYTPTSDSSSGSLPLIVYFHGGGFVYMAPDSKLLDDFCQRLAREISA 120
           DV +DASR+LWFRLY PTS+S S SLPLIVYFHGGGFVYMAPDSKLLDD CQRLAREI A
Sbjct: 61  DVIVDASRDLWFRLYAPTSESMSESLPLIVYFHGGGFVYMAPDSKLLDDLCQRLAREIPA 120

Query: 121 VVISVNYRLAPENRYSCQYEDGFDVLKFIDGDGSPFEGLPANVDLKRCFLAGDSAGGNIA 180
           VVISVNYRLAPE+R+ CQYED FDVLKFIDGD S  EG PANV+LKRCFLAGDSAGGNIA
Sbjct: 121 VVISVNYRLAPEHRHPCQYEDAFDVLKFIDGDSSAIEGFPANVNLKRCFLAGDSAGGNIA 180

Query: 181 HHTILKSAEHEFRELEIIGVIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYWKAFLPE 240
           HHTILKS +HE+RELEIIG+I+IQPFFGGE+R ESEK LIKAPLSTMDRTDWYWKAFLPE
Sbjct: 181 HHTILKSGDHEYRELEIIGLISIQPFFGGEKRMESEKRLIKAPLSTMDRTDWYWKAFLPE 240

Query: 241 GSDRDHPAVNIFGPNSVDISDVRFPAIKVFVGGLDPLIDWQKKYYEGLKKSGKEAYLTEY 300
           GSDRDHP+VNIFGPNSVDIS VRFPAIKVFVGGLDPL+DWQK+YYEGLKKSGKEAYL+EY
Sbjct: 241 GSDRDHPSVNIFGPNSVDISKVRFPAIKVFVGGLDPLMDWQKEYYEGLKKSGKEAYLSEY 300

Query: 301 PNAFHSFYGFPELPEWSLFIKDAADFVRTQCSKKSS 337
           PNAFHSFYGFPELPEWSLFIKD  DF+  QC KKSS
Sbjct: 301 PNAFHSFYGFPELPEWSLFIKDVRDFITEQCLKKSS 336

BLAST of Spg025428 vs. NCBI nr
Match: KAG6599120.1 (putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 619.8 bits (1597), Expect = 1.4e-173
Identity = 292/336 (86.90%), Postives = 312/336 (92.86%), Query Frame = 0

Query: 1   MSEALSGLRLPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSF 60
           MS+ALS LRLPWKVKMMLTAIAVGTDIC R D SVNR+L+NL+DFKSPPLKKPKNG+KSF
Sbjct: 1   MSDALSALRLPWKVKMMLTAIAVGTDICGRPDVSVNRFLANLMDFKSPPLKKPKNGVKSF 60

Query: 61  DVTIDASRNLWFRLYTPTSDSSSGSLPLIVYFHGGGFVYMAPDSKLLDDFCQRLAREISA 120
           DVT+DASR+LWFR+Y PTS+S+S SLPLIVYFHGGGFVYMAPDSKL DDFCQ LAREI A
Sbjct: 61  DVTVDASRSLWFRVYFPTSESTSESLPLIVYFHGGGFVYMAPDSKLYDDFCQSLAREIHA 120

Query: 121 VVISVNYRLAPENRYSCQYEDGFDVLKFIDGDGSPFEGLPANVDLKRCFLAGDSAGGNIA 180
           VVISVNYRLAPEN++ CQYED FDVLKFIDGDGS FEGLPANVDLKRCF+AGDSAGGNIA
Sbjct: 121 VVISVNYRLAPENKWPCQYEDSFDVLKFIDGDGSSFEGLPANVDLKRCFVAGDSAGGNIA 180

Query: 181 HHTILKSAEHEFRELEIIGVIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYWKAFLPE 240
           HH ILKS +HEFRELEIIG+IAIQPFFGGEERTES+  LIKAPLST+DRTDWYWKAFLPE
Sbjct: 181 HHMILKSGDHEFRELEIIGLIAIQPFFGGEERTESDIRLIKAPLSTIDRTDWYWKAFLPE 240

Query: 241 GSDRDHPAVNIFGPNSVDISDVRFPAIKVFVGGLDPLIDWQKKYYEGLKKSGKEAYLTEY 300
           G DRDHP+VNIFGP SVDIS VRFPAIKV VGGLDPLIDWQK+YYEGLKKSGKEAYLTEY
Sbjct: 241 GFDRDHPSVNIFGPKSVDISKVRFPAIKVLVGGLDPLIDWQKRYYEGLKKSGKEAYLTEY 300

Query: 301 PNAFHSFYGFPELPEWSLFIKDAADFVRTQCSKKSS 337
           PNAFHSFYGFPELPEWS FIKD ADF+  QCSKKSS
Sbjct: 301 PNAFHSFYGFPELPEWSEFIKDVADFIAAQCSKKSS 336

BLAST of Spg025428 vs. NCBI nr
Match: KAG7030059.1 (putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 618.6 bits (1594), Expect = 3.1e-173
Identity = 291/336 (86.61%), Postives = 312/336 (92.86%), Query Frame = 0

Query: 1   MSEALSGLRLPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSF 60
           MS+ALS LRLPWKVKMMLTAIAVGTDIC R D SVNR+L+NL+DFKSPPLKKPKNG+KSF
Sbjct: 1   MSDALSALRLPWKVKMMLTAIAVGTDICGRPDVSVNRFLANLMDFKSPPLKKPKNGVKSF 60

Query: 61  DVTIDASRNLWFRLYTPTSDSSSGSLPLIVYFHGGGFVYMAPDSKLLDDFCQRLAREISA 120
           DVT+DASR+LWFR+Y PTS+S+S SLPLIVYFHGGGFVYMAPDSKL DDFCQ LAREI A
Sbjct: 61  DVTVDASRSLWFRVYFPTSESTSESLPLIVYFHGGGFVYMAPDSKLYDDFCQSLAREIHA 120

Query: 121 VVISVNYRLAPENRYSCQYEDGFDVLKFIDGDGSPFEGLPANVDLKRCFLAGDSAGGNIA 180
           VVISVNYRLAPEN++ CQYED FDVLKFIDGDGS FEGLPANVDLKRCF+AGDSAGGNIA
Sbjct: 121 VVISVNYRLAPENKWPCQYEDSFDVLKFIDGDGSSFEGLPANVDLKRCFVAGDSAGGNIA 180

Query: 181 HHTILKSAEHEFRELEIIGVIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYWKAFLPE 240
           HH ILKS +HEFRELEIIG+IAIQPFFGGEERTES+  LIKAPLST+DRTDWYWKAFLPE
Sbjct: 181 HHMILKSGDHEFRELEIIGLIAIQPFFGGEERTESDIRLIKAPLSTIDRTDWYWKAFLPE 240

Query: 241 GSDRDHPAVNIFGPNSVDISDVRFPAIKVFVGGLDPLIDWQKKYYEGLKKSGKEAYLTEY 300
           G DRDHP+VNIFGP SVDIS VRFPAIKV VGGLDPLIDWQK+YYEGLKKSGKEAYLTEY
Sbjct: 241 GFDRDHPSVNIFGPKSVDISKVRFPAIKVLVGGLDPLIDWQKRYYEGLKKSGKEAYLTEY 300

Query: 301 PNAFHSFYGFPELPEWSLFIKDAADFVRTQCSKKSS 337
           PNAFHSFYGFPELPEWS FIKD ADF+  QCSKKS+
Sbjct: 301 PNAFHSFYGFPELPEWSEFIKDVADFIAAQCSKKST 336

BLAST of Spg025428 vs. NCBI nr
Match: XP_022999791.1 (probable carboxylesterase 18 [Cucurbita maxima])

HSP 1 Score: 618.6 bits (1594), Expect = 3.1e-173
Identity = 292/336 (86.90%), Postives = 307/336 (91.37%), Query Frame = 0

Query: 1   MSEALSGLRLPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSF 60
           MS+ALS LRLPWKVKMMLTAIAVGTDICCR D SVNR+L+NL+DFKSPPLKKPKNG+KSF
Sbjct: 1   MSDALSALRLPWKVKMMLTAIAVGTDICCRRDVSVNRFLANLMDFKSPPLKKPKNGVKSF 60

Query: 61  DVTIDASRNLWFRLYTPTSDSSSGSLPLIVYFHGGGFVYMAPDSKLLDDFCQRLAREISA 120
           DVT+D SRNLWFRLY PTS+S S SLPLI YFHGGGFVYMAPDSKLLDDFCQ LAREI A
Sbjct: 61  DVTVDVSRNLWFRLYAPTSESMSESLPLIFYFHGGGFVYMAPDSKLLDDFCQSLAREIHA 120

Query: 121 VVISVNYRLAPENRYSCQYEDGFDVLKFIDGDGSPFEGLPANVDLKRCFLAGDSAGGNIA 180
           VVISVNYRLAPE+   CQYED FDVLKFIDGDGS FEGLPANVDLKRCF+AGDSAGGNIA
Sbjct: 121 VVISVNYRLAPEHHCPCQYEDSFDVLKFIDGDGSSFEGLPANVDLKRCFVAGDSAGGNIA 180

Query: 181 HHTILKSAEHEFRELEIIGVIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYWKAFLPE 240
           HH ILKS EH FRELEIIG+IAIQPFFGGEERTESE  L+KAPLST+DRTDWYWKAFLPE
Sbjct: 181 HHMILKSGEHGFRELEIIGLIAIQPFFGGEERTESEIRLLKAPLSTIDRTDWYWKAFLPE 240

Query: 241 GSDRDHPAVNIFGPNSVDISDVRFPAIKVFVGGLDPLIDWQKKYYEGLKKSGKEAYLTEY 300
           G DRDHP+VNIFGP SVDIS VRFPAIKV VGGLDPLIDWQK+YYEGLKKSGKEAYLTEY
Sbjct: 241 GFDRDHPSVNIFGPKSVDISKVRFPAIKVLVGGLDPLIDWQKRYYEGLKKSGKEAYLTEY 300

Query: 301 PNAFHSFYGFPELPEWSLFIKDAADFVRTQCSKKSS 337
           PNAFHSFYGFPELPEW  FIKD ADF+  QCSKKSS
Sbjct: 301 PNAFHSFYGFPELPEWPEFIKDVADFIAAQCSKKSS 336

BLAST of Spg025428 vs. ExPASy Swiss-Prot
Match: Q9LT10 (Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 2.9e-97
Identity = 175/332 (52.71%), Postives = 226/332 (68.07%), Query Frame = 0

Query: 8   LRLPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSFDVTIDAS 67
           L LP K ++ LT I+  TD   R DG++NR    L DF++PP  KP N + + D  +D S
Sbjct: 11  LTLPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFVVDQS 70

Query: 68  RNLWFRLYTPTSDSSSGSLPLIVYFHGGGFVYMAPDSKLLDDFCQRLAREISAVVISVNY 127
           R+LWFRLYTP    S   +P++V+FHGGGF +++P++   D+ C+R AR++ A VISVNY
Sbjct: 71  RDLWFRLYTP--HVSGDKIPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISVNY 130

Query: 128 RLAPENRYSCQYEDGFDVLKFIDGDGSPFEGLPANVDLKRCFLAGDSAGGNIAHHT---I 187
           RLAPE+RY  QY+DGFD LK+I+ +      LPAN DL RCF AGDSAGGNIAH+    I
Sbjct: 131 RLAPEHRYPAQYDDGFDALKYIEENHGSI--LPANADLSRCFFAGDSAGGNIAHNVAIRI 190

Query: 188 LKSAEHEFRELEIIGVIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYWKAFLPEGSDR 247
            +     F  +++IG+I+IQPFFGGEERTE+EK+L+ APL + DRTDW WKA    G +R
Sbjct: 191 CREPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKAM---GLNR 250

Query: 248 DHPAVNIFGPNSVDISDVRFPAIKVFVGGLDPLIDWQKKYYEGLKKSGKEAYLTEYPNAF 307
           DH AVN+ GPN+VDIS + +P   V V G DPL DWQ+ YYE LK  GK+A L EYPN F
Sbjct: 251 DHEAVNVGGPNAVDISGLDYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIEYPNMF 310

Query: 308 HSFYGFPELPEWSLFIKDAADFVRTQCSKKSS 337
           H+FY FPELPE    I    DFV  + +  S+
Sbjct: 311 HAFYIFPELPEAGQLIMRIKDFVDERVASLSA 335

BLAST of Spg025428 vs. ExPASy Swiss-Prot
Match: Q940G6 (Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 2.0e-69
Identity = 139/335 (41.49%), Postives = 191/335 (57.01%), Query Frame = 0

Query: 10  LPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSFDVTIDASRN 69
           +P    ++++   +  ++  R DG+ NR+L+  LD K P    P NG+ SFDV ID   N
Sbjct: 16  VPLNTWVLISNFKLAYNLLRRPDGTFNRHLAEFLDRKVPANANPVNGVFSFDVIIDRQTN 75

Query: 70  LWFRLYTPTSDSSSGSL------------PLIVYFHGGGFVYMAPDSKLLDDFCQRLARE 129
           L  R+Y P    +S S+            P+IV+FHGG F + + +S + D  C+RL   
Sbjct: 76  LLSRVYRPADAGTSPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGL 135

Query: 130 ISAVVISVNYRLAPENRYSCQYEDGFDVLKFIDGDGSPFEGLPANVDLK-RCFLAGDSAG 189
             AVV+SVNYR APENRY C Y+DG+ VLK+++        L +  D K R FLAGDS+G
Sbjct: 136 CGAVVVSVNYRRAPENRYPCAYDDGWAVLKWVNSS----SWLRSKKDSKVRIFLAGDSSG 195

Query: 190 GNIAHHTILKSAEHEFRELEIIGVIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYWKA 249
           GNI H+  +++ E     ++++G I + P FGG ERTESEK L      T+   DWYW+A
Sbjct: 196 GNIVHNVAVRAVE---SRIDVLGNILLNPMFGGTERTESEKRLDGKYFVTVRDRDWYWRA 255

Query: 250 FLPEGSDRDHPAVNIFGPNSVDISDVRFPAIKVFVGGLDPLIDWQKKYYEGLKKSGKEAY 309
           FLPEG DR+HPA + FGP S  +  + FP   V V GLD + DWQ KY EGLKK+G+E  
Sbjct: 256 FLPEGEDREHPACSPFGPRSKSLEGLSFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVK 315

Query: 310 LTEYPNAFHSFYGFPELPEWSLFIKDAADFVRTQC 332
           L     A   FY  P    +   + + A FV  +C
Sbjct: 316 LLYLEQATIGFYLLPNNNHFHTVMDEIAAFVNAEC 343

BLAST of Spg025428 vs. ExPASy Swiss-Prot
Match: Q9MAA7 (Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 1.0e-62
Identity = 132/337 (39.17%), Postives = 180/337 (53.41%), Query Frame = 0

Query: 10  LPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSFDVTIDASRN 69
           +P    ++++   V  +I  R DG+ NR+L+  LD K      P +G+ SFDV ID   N
Sbjct: 16  VPLNTWVLISNFKVAYNILRRPDGTFNRHLAEYLDRKVTANANPVDGVFSFDVLIDRRIN 75

Query: 70  LWFRLYTPT--------------SDSSSGSLPLIVYFHGGGFVYMAPDSKLLDDFCQRLA 129
           L  R+Y P                      +P+I++FHGG F + + +S + D  C+RL 
Sbjct: 76  LLSRVYRPAYADQEQPPSILDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRLV 135

Query: 130 REISAVVISVNYRLAPENRYSCQYEDGFDVLKFIDGDGSPFEGLPANVDLK-RCFLAGDS 189
                VV+SVNYR APEN Y C Y+DG+  L +++        L +  D K   FLAGDS
Sbjct: 136 GLCKCVVVSVNYRRAPENPYPCAYDDGWIALNWVNSR----SWLKSKKDSKVHIFLAGDS 195

Query: 190 AGGNIAHHTILKSAEHEFRELEIIGVIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYW 249
           +GGNIAH+  L++ E     ++++G I + P FGG ERTESEK L      T+   DWYW
Sbjct: 196 SGGNIAHNVALRAGE---SGIDVLGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDWYW 255

Query: 250 KAFLPEGSDRDHPAVNIFGPNSVDISDVRFPAIKVFVGGLDPLIDWQKKYYEGLKKSGKE 309
           KAFLPEG DR+HPA N F P    +  V FP   V V GLD + DWQ  Y EGLKK+G+E
Sbjct: 256 KAFLPEGEDREHPACNPFSPRGKSLEGVSFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQE 315

Query: 310 AYLTEYPNAFHSFYGFPELPEWSLFIKDAADFVRTQC 332
             L     A   FY  P    +   + + + FV  +C
Sbjct: 316 VKLMHLEKATVGFYLLPNNNHFHNVMDEISAFVNAEC 345

BLAST of Spg025428 vs. ExPASy Swiss-Prot
Match: Q9LYC1 (Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 1.7e-60
Identity = 124/335 (37.01%), Postives = 186/335 (55.52%), Query Frame = 0

Query: 10  LPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSFDVTIDASRN 69
           +P    ++++   +   +  R DGS NR L+  LD K P    P +G+ SFD  +D++ N
Sbjct: 16  VPLNTWVLISNFKLAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFD-HVDSTTN 75

Query: 70  LWFRLYTPTS---------------DSSSGSLPLIVYFHGGGFVYMAPDSKLLDDFCQRL 129
           L  R+Y P S                S++  +P++++FHGG F + + +S + D FC+RL
Sbjct: 76  LLTRIYQPASLLHQTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRL 135

Query: 130 AREISAVVISVNYRLAPENRYSCQYEDGFDVLKFIDGDGSPFEGLPANVDLKRCFLAGDS 189
                 VV+SV+YR +PE+RY C Y+DG++ L ++        G  +NV +   +LAGDS
Sbjct: 136 VTICGVVVVSVDYRRSPEHRYPCAYDDGWNALNWVKSRVWLQSGKDSNVYV---YLAGDS 195

Query: 190 AGGNIAHHTILKSAEHEFRELEIIGVIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYW 249
           +GGNIAH+  +++       ++++G I + P FGG+ERT+SEK L      T+   DWYW
Sbjct: 196 SGGNIAHNVAVRATN---EGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRDWYW 255

Query: 250 KAFLPEGSDRDHPAVNIFGPNSVDISDVRFPAIKVFVGGLDPLIDWQKKYYEGLKKSGKE 309
           +A+LPEG DRDHPA N FGP    +  V FP   V V GLD + DWQ  Y +GLKK+G E
Sbjct: 256 RAYLPEGEDRDHPACNPFGPRGQSLKGVNFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLE 315

Query: 310 AYLTEYPNAFHSFYGFPELPEWSLFIKDAADFVRT 330
             L     A   FY  P    +   +++   FV +
Sbjct: 316 VNLLYLKQATIGFYFLPNNDHFHCLMEELNKFVHS 343

BLAST of Spg025428 vs. ExPASy Swiss-Prot
Match: Q6L545 (Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 3.3e-56
Identity = 119/339 (35.10%), Postives = 177/339 (52.21%), Query Frame = 0

Query: 10  LPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSFDVTIDASRN 69
           +P    ++++   +  +I  R+DG+  R L   LD + P   +P  G+ SFD  ID S  
Sbjct: 16  VPLHTWVLISNFKLSYNILRRADGTFERDLGEYLDRRVPANARPLEGVSSFDHIIDQSVG 75

Query: 70  LWFRLYTPTS--DSSSGS-------------------LPLIVYFHGGGFVYMAPDSKLLD 129
           L  R+Y   +  D+  G+                    P+I++FHGG FV+ +  S + D
Sbjct: 76  LEVRIYRAAAEGDAEEGAAAVTRPILEFLTDAPAAEPFPVIIFFHGGSFVHSSASSTIYD 135

Query: 130 DFCQRLAREISAVVISVNYRLAPENRYSCQYEDGFDVLKFIDGDGSPFEGLPANVDLKRC 189
             C+R  +    VV+SVNYR APE+RY C Y+DG+  LK++        G  A     R 
Sbjct: 136 SLCRRFVKLSKGVVVSVNYRRAPEHRYPCAYDDGWTALKWVMSQPFMRSGGDAQA---RV 195

Query: 190 FLAGDSAGGNIAHHTILKSAEHEFRELEIIGVIAIQPFFGGEERTESEKELIKAPLSTMD 249
           FL+GDS+GGNIAHH  +++A+     +++ G I +   FGG ERTESE+ L      T+ 
Sbjct: 196 FLSGDSSGGNIAHHVAVRAAD---EGVKVCGNILLNAMFGGTERTESERRLDGKYFVTLQ 255

Query: 250 RTDWYWKAFLPEGSDRDHPAVNIFGPNSVDISDVRFPAIKVFVGGLDPLIDWQKKYYEGL 309
             DWYWKA+LPE +DRDHPA N FGPN   +  + F    + V GLD   D Q  Y + L
Sbjct: 256 DRDWYWKAYLPEDADRDHPACNPFGPNGRRLGGLPFAKSLIIVSGLDLTCDRQLAYADAL 315

Query: 310 KKSGKEAYLTEYPNAFHSFYGFPELPEWSLFIKDAADFV 328
           ++ G    + +  NA   FY  P    +   +++ +DF+
Sbjct: 316 REDGHHVKVVQCENATVGFYLLPNTVHYHEVMEEISDFL 348

BLAST of Spg025428 vs. ExPASy TrEMBL
Match: A0A6J1KE39 (probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111494035 PE=4 SV=1)

HSP 1 Score: 618.6 bits (1594), Expect = 1.5e-173
Identity = 292/336 (86.90%), Postives = 307/336 (91.37%), Query Frame = 0

Query: 1   MSEALSGLRLPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSF 60
           MS+ALS LRLPWKVKMMLTAIAVGTDICCR D SVNR+L+NL+DFKSPPLKKPKNG+KSF
Sbjct: 1   MSDALSALRLPWKVKMMLTAIAVGTDICCRRDVSVNRFLANLMDFKSPPLKKPKNGVKSF 60

Query: 61  DVTIDASRNLWFRLYTPTSDSSSGSLPLIVYFHGGGFVYMAPDSKLLDDFCQRLAREISA 120
           DVT+D SRNLWFRLY PTS+S S SLPLI YFHGGGFVYMAPDSKLLDDFCQ LAREI A
Sbjct: 61  DVTVDVSRNLWFRLYAPTSESMSESLPLIFYFHGGGFVYMAPDSKLLDDFCQSLAREIHA 120

Query: 121 VVISVNYRLAPENRYSCQYEDGFDVLKFIDGDGSPFEGLPANVDLKRCFLAGDSAGGNIA 180
           VVISVNYRLAPE+   CQYED FDVLKFIDGDGS FEGLPANVDLKRCF+AGDSAGGNIA
Sbjct: 121 VVISVNYRLAPEHHCPCQYEDSFDVLKFIDGDGSSFEGLPANVDLKRCFVAGDSAGGNIA 180

Query: 181 HHTILKSAEHEFRELEIIGVIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYWKAFLPE 240
           HH ILKS EH FRELEIIG+IAIQPFFGGEERTESE  L+KAPLST+DRTDWYWKAFLPE
Sbjct: 181 HHMILKSGEHGFRELEIIGLIAIQPFFGGEERTESEIRLLKAPLSTIDRTDWYWKAFLPE 240

Query: 241 GSDRDHPAVNIFGPNSVDISDVRFPAIKVFVGGLDPLIDWQKKYYEGLKKSGKEAYLTEY 300
           G DRDHP+VNIFGP SVDIS VRFPAIKV VGGLDPLIDWQK+YYEGLKKSGKEAYLTEY
Sbjct: 241 GFDRDHPSVNIFGPKSVDISKVRFPAIKVLVGGLDPLIDWQKRYYEGLKKSGKEAYLTEY 300

Query: 301 PNAFHSFYGFPELPEWSLFIKDAADFVRTQCSKKSS 337
           PNAFHSFYGFPELPEW  FIKD ADF+  QCSKKSS
Sbjct: 301 PNAFHSFYGFPELPEWPEFIKDVADFIAAQCSKKSS 336

BLAST of Spg025428 vs. ExPASy TrEMBL
Match: A0A6J1G4S5 (probable carboxylesterase 18 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450746 PE=4 SV=1)

HSP 1 Score: 614.0 bits (1582), Expect = 3.7e-172
Identity = 289/336 (86.01%), Postives = 311/336 (92.56%), Query Frame = 0

Query: 1   MSEALSGLRLPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSF 60
           MS+ALS LRLPWKVKMMLTAIAVGTDIC R D SVNR+L+NL+DFKSPPLKKPKNG+KSF
Sbjct: 1   MSDALSALRLPWKVKMMLTAIAVGTDICGRPDVSVNRFLANLMDFKSPPLKKPKNGVKSF 60

Query: 61  DVTIDASRNLWFRLYTPTSDSSSGSLPLIVYFHGGGFVYMAPDSKLLDDFCQRLAREISA 120
           DVT+DASRNLWFRLY P+S+S+S SLPLI YFHGGGFVYMAPDSKLLDDFCQ LAREI A
Sbjct: 61  DVTVDASRNLWFRLYFPSSESTSESLPLIFYFHGGGFVYMAPDSKLLDDFCQSLAREIHA 120

Query: 121 VVISVNYRLAPENRYSCQYEDGFDVLKFIDGDGSPFEGLPANVDLKRCFLAGDSAGGNIA 180
           VVISVNYRLAPEN+  CQYED FDVLKFIDG+GS FEGLPANVDLKRCF+AGDSAGGNIA
Sbjct: 121 VVISVNYRLAPENKCPCQYEDSFDVLKFIDGEGSSFEGLPANVDLKRCFVAGDSAGGNIA 180

Query: 181 HHTILKSAEHEFRELEIIGVIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYWKAFLPE 240
           HH ILKS +HEFR+LEIIG+IAIQPFFGGEERTES+  LIKAPLST+DRTDWYWKAFLPE
Sbjct: 181 HHMILKSGDHEFRKLEIIGLIAIQPFFGGEERTESDIRLIKAPLSTIDRTDWYWKAFLPE 240

Query: 241 GSDRDHPAVNIFGPNSVDISDVRFPAIKVFVGGLDPLIDWQKKYYEGLKKSGKEAYLTEY 300
           G DRDHP+VNIFGP SVDIS+VRFPAIKV VGGLDPLIDWQK+YYEGLKKSGKEAYLTEY
Sbjct: 241 GFDRDHPSVNIFGPKSVDISNVRFPAIKVVVGGLDPLIDWQKRYYEGLKKSGKEAYLTEY 300

Query: 301 PNAFHSFYGFPELPEWSLFIKDAADFVRTQCSKKSS 337
           PNAFHSFYGFPELPEWS FIKD ADF+  QC K+SS
Sbjct: 301 PNAFHSFYGFPELPEWSEFIKDVADFIAAQCKKQSS 336

BLAST of Spg025428 vs. ExPASy TrEMBL
Match: A0A6J1KHY2 (probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111493993 PE=4 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 2.6e-165
Identity = 276/336 (82.14%), Postives = 304/336 (90.48%), Query Frame = 0

Query: 1   MSEALSGLRLPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSF 60
           MS+ALS LRLPWKVK++LT+I +GTDIC R DGSVNR++ NL+DFKS PLKKPKNG+KSF
Sbjct: 1   MSDALSALRLPWKVKIILTSITIGTDICSRPDGSVNRFIFNLMDFKSSPLKKPKNGVKSF 60

Query: 61  DVTIDASRNLWFRLYTPTSDSSSGSLPLIVYFHGGGFVYMAPDSKLLDDFCQRLAREISA 120
           DVT+DASRNLWFRLY PTS+S+S SLPLIVYFHGGGFVYMAPDSKL DDFCQ L+REI A
Sbjct: 61  DVTVDASRNLWFRLYFPTSESTSESLPLIVYFHGGGFVYMAPDSKLYDDFCQSLSREIHA 120

Query: 121 VVISVNYRLAPENRYSCQYEDGFDVLKFIDGDGSPFEGLPANVDLKRCFLAGDSAGGNIA 180
           VVISVNYRLAPEN++ CQYED FDVLKFIDGDGS FEGL ANVDLKRCF+AGDSAGGNIA
Sbjct: 121 VVISVNYRLAPENKWPCQYEDSFDVLKFIDGDGSSFEGLAANVDLKRCFVAGDSAGGNIA 180

Query: 181 HHTILKSAEHEFRELEIIGVIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYWKAFLPE 240
           HH ILKS +HEFR+LEIIG+IAIQPFFGGEERTESE  L+KAPL T+DR DW+WKAFLPE
Sbjct: 181 HHMILKSGDHEFRKLEIIGLIAIQPFFGGEERTESEIRLLKAPLLTIDRADWFWKAFLPE 240

Query: 241 GSDRDHPAVNIFGPNSVDISDVRFPAIKVFVGGLDPLIDWQKKYYEGLKKSGKEAYLTEY 300
           G DRDH +VNIFGP S DIS VRFPAIKVFVGGLDPLID QK+YYEGLKKSGKEAYLTEY
Sbjct: 241 GFDRDHKSVNIFGPKSEDISKVRFPAIKVFVGGLDPLIDRQKRYYEGLKKSGKEAYLTEY 300

Query: 301 PNAFHSFYGFPELPEWSLFIKDAADFVRTQCSKKSS 337
           PNAFH+FYGFPELPEWS FIKD ADF+  +C K+SS
Sbjct: 301 PNAFHTFYGFPELPEWSKFIKDVADFIAAECKKQSS 336

BLAST of Spg025428 vs. ExPASy TrEMBL
Match: A0A5A7T6B5 (Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold119G00400 PE=4 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 9.9e-165
Identity = 273/336 (81.25%), Postives = 304/336 (90.48%), Query Frame = 0

Query: 1   MSEALSGLRLPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSF 60
           MS+ALS LRLPWK+KM+L+AI++GTDICCRSD SVNR+L+N LDFKSPPLKKPKNG+KSF
Sbjct: 1   MSDALSSLRLPWKIKMILSAISLGTDICCRSDVSVNRFLANFLDFKSPPLKKPKNGVKSF 60

Query: 61  DVTIDASRNLWFRLYTPTSDSSSGSLPLIVYFHGGGFVYMAPDSKLLDDFCQRLAREISA 120
           D T+D+SRNLWFRLYTPT++S+S SLPLIVY HGGGFVYMAPDSKLLD+ CQRLAREI A
Sbjct: 61  DTTVDSSRNLWFRLYTPTTESTSESLPLIVYCHGGGFVYMAPDSKLLDELCQRLAREIPA 120

Query: 121 VVISVNYRLAPENRYSCQYEDGFDVLKFIDGDGSPFEGLPANVDLKRCFLAGDSAGGNIA 180
           VVIS+NYRLAPE+RY CQYED FD+LKFID + S  EG P NVDLKRCFLAGDSAGGNIA
Sbjct: 121 VVISLNYRLAPEHRYPCQYEDAFDILKFIDNNASAIEGFPPNVDLKRCFLAGDSAGGNIA 180

Query: 181 HHTILKSAEHEFRELEIIGVIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYWKAFLPE 240
           HH ILKS +HE RELEIIG+I+IQPFFGGEER ESE +LIKAPLST+DRTDWYWKAFLPE
Sbjct: 181 HHVILKSGDHEHRELEIIGLISIQPFFGGEERLESEIKLIKAPLSTIDRTDWYWKAFLPE 240

Query: 241 GSDRDHPAVNIFGPNSVDISDVRFPAIKVFVGGLDPLIDWQKKYYEGLKKSGKEAYLTEY 300
           G DRDHP+VN+FGPNS DIS+VRFPA KV VGGLDPLIDWQK+YYEGLKKSGKEAYL+EY
Sbjct: 241 GCDRDHPSVNVFGPNSTDISNVRFPATKVLVGGLDPLIDWQKRYYEGLKKSGKEAYLSEY 300

Query: 301 PNAFHSFYGFPELPEWSLFIKDAADFVRTQCSKKSS 337
           PN FHSFYGFPEL E +LFIKD  DFV+ QC KKSS
Sbjct: 301 PNGFHSFYGFPELEESNLFIKDIRDFVKEQCLKKSS 336

BLAST of Spg025428 vs. ExPASy TrEMBL
Match: A0A6J1G2I0 (probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111450214 PE=4 SV=1)

HSP 1 Score: 588.6 bits (1516), Expect = 1.7e-164
Identity = 274/336 (81.55%), Postives = 304/336 (90.48%), Query Frame = 0

Query: 1   MSEALSGLRLPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSF 60
           MS+ALS LRLPWKVK++LT+I +GTDIC R DGSVNR++ NL+DFKS PLKKPKNG+KSF
Sbjct: 1   MSDALSALRLPWKVKIILTSITIGTDICSRPDGSVNRFIFNLMDFKSSPLKKPKNGVKSF 60

Query: 61  DVTIDASRNLWFRLYTPTSDSSSGSLPLIVYFHGGGFVYMAPDSKLLDDFCQRLAREISA 120
           DVT+DASRNLWFR+Y PTS+S+S SLPLIVYFHGGGFVYMAPDSKL DDFCQ L+REI A
Sbjct: 61  DVTVDASRNLWFRVYFPTSESTSESLPLIVYFHGGGFVYMAPDSKLYDDFCQSLSREIHA 120

Query: 121 VVISVNYRLAPENRYSCQYEDGFDVLKFIDGDGSPFEGLPANVDLKRCFLAGDSAGGNIA 180
           VVISVNYRLAPEN++  QYED FDVLKFIDGDGS FEGLPANVDLKRCF+AGDSAGGNIA
Sbjct: 121 VVISVNYRLAPENKWPRQYEDSFDVLKFIDGDGSSFEGLPANVDLKRCFVAGDSAGGNIA 180

Query: 181 HHTILKSAEHEFRELEIIGVIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYWKAFLPE 240
           HH ILKS +HEFR+LEIIG+IAIQPFFGGEERTESE  L+KAPL T+DR DW+WKAFLPE
Sbjct: 181 HHMILKSGDHEFRKLEIIGLIAIQPFFGGEERTESEIRLLKAPLLTIDRADWFWKAFLPE 240

Query: 241 GSDRDHPAVNIFGPNSVDISDVRFPAIKVFVGGLDPLIDWQKKYYEGLKKSGKEAYLTEY 300
           G DRDHP+VNIFGP S DIS VRFPAIKVFVGGLDPLID QK+YYEGLKKSGKEAY+ EY
Sbjct: 241 GFDRDHPSVNIFGPKSEDISKVRFPAIKVFVGGLDPLIDRQKRYYEGLKKSGKEAYIIEY 300

Query: 301 PNAFHSFYGFPELPEWSLFIKDAADFVRTQCSKKSS 337
           PNAFH+FYGFPELPEWS FIKD ADF+  +C K+SS
Sbjct: 301 PNAFHTFYGFPELPEWSKFIKDVADFIAAECKKQSS 336

BLAST of Spg025428 vs. TAIR 10
Match: AT5G23530.1 (carboxyesterase 18 )

HSP 1 Score: 356.7 bits (914), Expect = 2.1e-98
Identity = 175/332 (52.71%), Postives = 226/332 (68.07%), Query Frame = 0

Query: 8   LRLPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSFDVTIDAS 67
           L LP K ++ LT I+  TD   R DG++NR    L DF++PP  KP N + + D  +D S
Sbjct: 11  LTLPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFVVDQS 70

Query: 68  RNLWFRLYTPTSDSSSGSLPLIVYFHGGGFVYMAPDSKLLDDFCQRLAREISAVVISVNY 127
           R+LWFRLYTP    S   +P++V+FHGGGF +++P++   D+ C+R AR++ A VISVNY
Sbjct: 71  RDLWFRLYTP--HVSGDKIPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISVNY 130

Query: 128 RLAPENRYSCQYEDGFDVLKFIDGDGSPFEGLPANVDLKRCFLAGDSAGGNIAHHT---I 187
           RLAPE+RY  QY+DGFD LK+I+ +      LPAN DL RCF AGDSAGGNIAH+    I
Sbjct: 131 RLAPEHRYPAQYDDGFDALKYIEENHGSI--LPANADLSRCFFAGDSAGGNIAHNVAIRI 190

Query: 188 LKSAEHEFRELEIIGVIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYWKAFLPEGSDR 247
            +     F  +++IG+I+IQPFFGGEERTE+EK+L+ APL + DRTDW WKA    G +R
Sbjct: 191 CREPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKAM---GLNR 250

Query: 248 DHPAVNIFGPNSVDISDVRFPAIKVFVGGLDPLIDWQKKYYEGLKKSGKEAYLTEYPNAF 307
           DH AVN+ GPN+VDIS + +P   V V G DPL DWQ+ YYE LK  GK+A L EYPN F
Sbjct: 251 DHEAVNVGGPNAVDISGLDYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIEYPNMF 310

Query: 308 HSFYGFPELPEWSLFIKDAADFVRTQCSKKSS 337
           H+FY FPELPE    I    DFV  + +  S+
Sbjct: 311 HAFYIFPELPEAGQLIMRIKDFVDERVASLSA 335

BLAST of Spg025428 vs. TAIR 10
Match: AT5G27320.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 264.2 bits (674), Expect = 1.4e-70
Identity = 139/335 (41.49%), Postives = 191/335 (57.01%), Query Frame = 0

Query: 10  LPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSFDVTIDASRN 69
           +P    ++++   +  ++  R DG+ NR+L+  LD K P    P NG+ SFDV ID   N
Sbjct: 16  VPLNTWVLISNFKLAYNLLRRPDGTFNRHLAEFLDRKVPANANPVNGVFSFDVIIDRQTN 75

Query: 70  LWFRLYTPTSDSSSGSL------------PLIVYFHGGGFVYMAPDSKLLDDFCQRLARE 129
           L  R+Y P    +S S+            P+IV+FHGG F + + +S + D  C+RL   
Sbjct: 76  LLSRVYRPADAGTSPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGL 135

Query: 130 ISAVVISVNYRLAPENRYSCQYEDGFDVLKFIDGDGSPFEGLPANVDLK-RCFLAGDSAG 189
             AVV+SVNYR APENRY C Y+DG+ VLK+++        L +  D K R FLAGDS+G
Sbjct: 136 CGAVVVSVNYRRAPENRYPCAYDDGWAVLKWVNSS----SWLRSKKDSKVRIFLAGDSSG 195

Query: 190 GNIAHHTILKSAEHEFRELEIIGVIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYWKA 249
           GNI H+  +++ E     ++++G I + P FGG ERTESEK L      T+   DWYW+A
Sbjct: 196 GNIVHNVAVRAVE---SRIDVLGNILLNPMFGGTERTESEKRLDGKYFVTVRDRDWYWRA 255

Query: 250 FLPEGSDRDHPAVNIFGPNSVDISDVRFPAIKVFVGGLDPLIDWQKKYYEGLKKSGKEAY 309
           FLPEG DR+HPA + FGP S  +  + FP   V V GLD + DWQ KY EGLKK+G+E  
Sbjct: 256 FLPEGEDREHPACSPFGPRSKSLEGLSFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVK 315

Query: 310 LTEYPNAFHSFYGFPELPEWSLFIKDAADFVRTQC 332
           L     A   FY  P    +   + + A FV  +C
Sbjct: 316 LLYLEQATIGFYLLPNNNHFHTVMDEIAAFVNAEC 343

BLAST of Spg025428 vs. TAIR 10
Match: AT3G05120.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 241.9 bits (616), Expect = 7.4e-64
Identity = 132/337 (39.17%), Postives = 180/337 (53.41%), Query Frame = 0

Query: 10  LPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSFDVTIDASRN 69
           +P    ++++   V  +I  R DG+ NR+L+  LD K      P +G+ SFDV ID   N
Sbjct: 16  VPLNTWVLISNFKVAYNILRRPDGTFNRHLAEYLDRKVTANANPVDGVFSFDVLIDRRIN 75

Query: 70  LWFRLYTPT--------------SDSSSGSLPLIVYFHGGGFVYMAPDSKLLDDFCQRLA 129
           L  R+Y P                      +P+I++FHGG F + + +S + D  C+RL 
Sbjct: 76  LLSRVYRPAYADQEQPPSILDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRLV 135

Query: 130 REISAVVISVNYRLAPENRYSCQYEDGFDVLKFIDGDGSPFEGLPANVDLK-RCFLAGDS 189
                VV+SVNYR APEN Y C Y+DG+  L +++        L +  D K   FLAGDS
Sbjct: 136 GLCKCVVVSVNYRRAPENPYPCAYDDGWIALNWVNSR----SWLKSKKDSKVHIFLAGDS 195

Query: 190 AGGNIAHHTILKSAEHEFRELEIIGVIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYW 249
           +GGNIAH+  L++ E     ++++G I + P FGG ERTESEK L      T+   DWYW
Sbjct: 196 SGGNIAHNVALRAGE---SGIDVLGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDWYW 255

Query: 250 KAFLPEGSDRDHPAVNIFGPNSVDISDVRFPAIKVFVGGLDPLIDWQKKYYEGLKKSGKE 309
           KAFLPEG DR+HPA N F P    +  V FP   V V GLD + DWQ  Y EGLKK+G+E
Sbjct: 256 KAFLPEGEDREHPACNPFSPRGKSLEGVSFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQE 315

Query: 310 AYLTEYPNAFHSFYGFPELPEWSLFIKDAADFVRTQC 332
             L     A   FY  P    +   + + + FV  +C
Sbjct: 316 VKLMHLEKATVGFYLLPNNNHFHNVMDEISAFVNAEC 345

BLAST of Spg025428 vs. TAIR 10
Match: AT3G63010.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 234.6 bits (597), Expect = 1.2e-61
Identity = 124/335 (37.01%), Postives = 186/335 (55.52%), Query Frame = 0

Query: 10  LPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSFDVTIDASRN 69
           +P    ++++   +   +  R DGS NR L+  LD K P    P +G+ SFD  +D++ N
Sbjct: 16  VPLNTWVLISNFKLAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFD-HVDSTTN 75

Query: 70  LWFRLYTPTS---------------DSSSGSLPLIVYFHGGGFVYMAPDSKLLDDFCQRL 129
           L  R+Y P S                S++  +P++++FHGG F + + +S + D FC+RL
Sbjct: 76  LLTRIYQPASLLHQTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRL 135

Query: 130 AREISAVVISVNYRLAPENRYSCQYEDGFDVLKFIDGDGSPFEGLPANVDLKRCFLAGDS 189
                 VV+SV+YR +PE+RY C Y+DG++ L ++        G  +NV +   +LAGDS
Sbjct: 136 VTICGVVVVSVDYRRSPEHRYPCAYDDGWNALNWVKSRVWLQSGKDSNVYV---YLAGDS 195

Query: 190 AGGNIAHHTILKSAEHEFRELEIIGVIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYW 249
           +GGNIAH+  +++       ++++G I + P FGG+ERT+SEK L      T+   DWYW
Sbjct: 196 SGGNIAHNVAVRATN---EGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRDWYW 255

Query: 250 KAFLPEGSDRDHPAVNIFGPNSVDISDVRFPAIKVFVGGLDPLIDWQKKYYEGLKKSGKE 309
           +A+LPEG DRDHPA N FGP    +  V FP   V V GLD + DWQ  Y +GLKK+G E
Sbjct: 256 RAYLPEGEDRDHPACNPFGPRGQSLKGVNFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLE 315

Query: 310 AYLTEYPNAFHSFYGFPELPEWSLFIKDAADFVRT 330
             L     A   FY  P    +   +++   FV +
Sbjct: 316 VNLLYLKQATIGFYFLPNNDHFHCLMEELNKFVHS 343

BLAST of Spg025428 vs. TAIR 10
Match: AT5G62180.1 (carboxyesterase 20 )

HSP 1 Score: 185.3 bits (469), Expect = 8.2e-47
Identity = 107/273 (39.19%), Postives = 153/273 (56.04%), Query Frame = 0

Query: 32  DGSVNRYLSNLLDFKSPPLKKPKNGLKSFDVTIDASRNLWFRLYTPTS-----DSSSGSL 91
           DGS+ R LSN     + P   P N   S D+ ++  ++ W RLY P+S     + SS  L
Sbjct: 21  DGSITRDLSNFPCTAATPDPSPLNPAVSKDLPVNQLKSTWLRLYLPSSAVNEGNVSSQKL 80

Query: 92  PLIVYFHGGGFVYMAPDSKLLDDFCQRLAREISAVVISVNYRLAPENRYSCQYEDGFDVL 151
           P++VY+HGGGF+  + D +L  DFC  +AR+++A+V+S +YRLAPE+R    Y+DG + L
Sbjct: 81  PIVVYYHGGGFILCSVDMQLFHDFCSEVARDLNAIVVSPSYRLAPEHRLPAAYDDGVEAL 140

Query: 152 KFIDGDGSPFEGLPANVDLKRCFLAGDSAGGNIAHHTILKSAE--HEFRELEIIGVIAIQ 211
            +I    S  E + ++ D    FL G SAGGN+A++  L+S +   +   L+I G+I   
Sbjct: 141 DWI--KTSDDEWIKSHADFSNVFLMGTSAGGNLAYNVGLRSVDSVSDLSPLQIRGLILHH 200

Query: 212 PFFGGEERTESEKELIKAPLSTMDRTDWYWKAFLPEGSDRDHPAVN-IFGPNSVDISDVR 271
           PFFGGEER+ESE  L+   +     TD  W   LP G DRDH   N   G  S  +  + 
Sbjct: 201 PFFGGEERSESEIRLMNDQVCPPIVTDVMWDLSLPVGVDRDHEYSNPTVGDGSEKLEKIG 260

Query: 272 FPAIKVFV--GGLDPLIDWQKKYYEGLKKSGKE 295
               KV +  G  DP+ID QK   + +KK G E
Sbjct: 261 RLRWKVMMIGGEDDPMIDLQKDVAKLMKKKGVE 291