Spg025369 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg025369
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionAB hydrolase-1 domain-containing protein
Locationscaffold13: 41157692 .. 41160289 (-)
RNA-Seq ExpressionSpg025369
SyntenySpg025369
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCATGTTTCTTCCGTGTTTTCATTCTCGTAATAAAAGAGTTCACAACAACTAACAAGTAGACTTGAAAAGCCGATCGAATAAAGATTCGCCGTTCCGCTCCTTAAAAGCTCACGCCATTATCGGCGCCGCCCAAATCTCTTTCAAATGTGGCCGTCGTTTCTCAGCCTCGTGTCCTTGTACGGCGGCTATATCCGCCGCTGCTTCACTTCCGCCGGCCTTTTCCAGCAAGTCATTGACATCGACACCGAAACCACCATCGCCTTTTGGGGCCCAAAGGCCAAAAAGTCCACCGCAAAACCCGCAGTCCTCCTCCTCCACGGCTTCGGCCCGATGGCGATTTGGCAATGGCGTCAACAGGTCCAATTCCTCGCCGATGATTTCGACGTCTACGTCCCCGACCTCGTCTTCTTCGGCGGCTCAACGACCAAATCGGCCGACCGGACGGAGGTTTTTCAGGCGATTTCGATCGGAAAGCTCATGGAGAAGATCGGAGTGGAAAAATACTCGGCGATGGGAACGAGCTACGGCGGATTTGTGGCGTATCATATGGCGAGGATTTGGCCGGAGAGGATCGAGAAGGTTATAATCGCGAGCTCCGGCGTTAATTTGAGACGGAAAGATAACGACGCGATGTTGAAAAGAGCTAACGTGGAGAAGATTGACGACTTTTTGCTGCCGGTGACGGCGGAACAATTACGGACGTTAATGAAGCTCGCCGTATTCAAGGGAGTGGGCAGAAGGATGCCGGACTTCTTCTTGAACGACTTTATCCATGTAAGGACAAAAATGGTTACATAAAAACTTTTTTTCTTTTTTCTTTTTTTTTTCTTTAATTTAAAAAATTATAAAAATCTCTCGAGGTATTCTGCATTCACTAATTTAAAAAGATACCGAATAACATGAGATCAACATTTAAATATAAATCACTCAAAAATTTAAAATTTCAATGCATAATTATAATTTTTTAAAGTTAATTAAAAAATAATTGCCTCTATCCGTCCCTCATTTTTTGCTGGAAATTATTTGGTTATTATTTGGCATTTTTTTCCTATATGGGATTTTTCTACATATCACCATTAAAATGTTGACAATCGGAAAAAATAATTTTTCTTTCTTAATTGTCAAGATTTTAATGGAGTATGTAGAGAGATGACTATGCAGCCAAGTATTAATCTTTTTTGCTATGATATCTATGTTTATGTGTGTTCTCTTTCTATGGTGAACCCTAAGTAATTATTTTGGATTTGGAGTTATTTTTTGAAAGGCAGATGATGATGTTGATTTGTTTGGTGAACTTAGAATTGCATCTAAGGTCAAATAATTATGGTTTTTATTAAGTTATTGGTAGGAAAAACCTTCCCAATTATTTTATTTTTCTCTATAATTTAAAATTTTAAAGAGTAATTGGGCGACGTTTGGTTTGACCTTTTAAAAAAATAAGATAAAATAAAACAAACATCGCAAATTGCATTTTTTTTTATTGGTTTGAAAAATACATCGCAAGTTGCATATGCATGGAGTTTAAATTATTTTGATAATTAATATAATTTTGCAACAGGTGATAGTCTTTTTTTTTTGGCTAAATTTTATGTATTGTCTACATATTATAGGTCTATTTTTAATTCAGAAAGTAAGTAAAATGTATGAAATTGGAGTAATAATGTGTGTATAAATCCAGAAATTATACATGGAAAACAGGGAGGAAAAGATTGAACTCCTAAAGTCGCTCACACTTGGAAAAGAAGAGACCATCAACCTCTTTCCACTTTCTCAGGTGAATTATACATTTTATGTAGAATCTTCTCATCAGTCATCGAGATTATTAAATGTGACTGTAACAATGAAACTGACAATTTTACATTACACGACAATGGTATCTAATAATTTTCTTTTCTTTCACATAGAGAACTCCGATATATGTTTGTTCACGTTTGTAATTTTGGTAAGTGAATTGAATTGAATTGATGTGCAGGAGGTATTGATAATATGGGGAGATCATGACCAGCTATTTCCCTTGGAAATGGCAAAAGAGCTCAAAGAGTAAGATTTTCTCTCTCTCTTTTTTCTTTTTAAACCATTTAATTTGTTGAGATTTTGGTGGGCTTAACGTTTAGAAATGTGTTAGCAGGATTCTTGGGGAGAAGACAAGGCTGGAGGTGATGAAGGAGACCTCACACGTGCCTCAGATGGAAGCTCCTGCAGAATTCAATCGTCTTGTCAAGAGTTTCTTATGAGTAATTATTGGAAAATACTGGCTATATGTCGGTACGTAAGGATCTTTCTATACATCACCATTATTAAAATGTTGACTCTCAGGAGAGAGATGAAATTTTTTCTCATTTTTTACCTTTTTCTTTTTTTAGTTGTTAAAAATTATTAATGAAACGTAGGAAGATCCTCACATATCGATGTGCAGTCAAGTATCGTTCTTAATTATTTCTTCCTCCCACCTCTCTTAGTGCTACGTTTGATGAACCATAGAGAATGTAAAATTCTGTTTCTTATGCCCAATTTCCAGCCCCATGTGTAAAGTTTTTTAATTTTATTTTTATTTTGGTGAAACGAACTATTAGGAGTTGTTGGTGCGAAATGGGTC

mRNA sequence

ATGTGGCCGTCGTTTCTCAGCCTCGTGTCCTTGTACGGCGGCTATATCCGCCGCTGCTTCACTTCCGCCGGCCTTTTCCAGCAAGTCATTGACATCGACACCGAAACCACCATCGCCTTTTGGGGCCCAAAGGCCAAAAAGTCCACCGCAAAACCCGCAGTCCTCCTCCTCCACGGCTTCGGCCCGATGGCGATTTGGCAATGGCGTCAACAGGTCCAATTCCTCGCCGATGATTTCGACGTCTACGTCCCCGACCTCGTCTTCTTCGGCGGCTCAACGACCAAATCGGCCGACCGGACGGAGGTTTTTCAGGCGATTTCGATCGGAAAGCTCATGGAGAAGATCGGAGTGGAAAAATACTCGGCGATGGGAACGAGCTACGGCGGATTTGTGGCGTATCATATGGCGAGGATTTGGCCGGAGAGGATCGAGAAGGTTATAATCGCGAGCTCCGGCGTTAATTTGAGACGGAAAGATAACGACGCGATGTTGAAAAGAGCTAACGTGGAGAAGATTGACGACTTTTTGCTGCCGGTGACGGCGGAACAATTACGGACGTTAATGAAGCTCGCCGTATTCAAGGGAGTGGGCAGAAGGATGCCGGACTTCTTCTTGAACGACTTTATCCATGCAGATGATGATGTTGATTTGTTTGGTGAACTTAGAATTGCATCTAAGAAATTATACATGGAAAACAGGGAGGAAAAGATTGAACTCCTAAAGTCGCTCACACTTGGAAAAGAAGAGACCATCAACCTCTTTCCACTTTCTCAGGAGGTATTGATAATATGGGGAGATCATGACCAGCTATTTCCCTTGGAAATGGCAAAAGAGCTCAAAGAGATTCTTGGGGAGAAGACAAGGCTGGAGGTGATGAAGGAGACCTCACACGTGCCTCAGATGGAAGCTCCTGCAGAATTCAATCGTCTTGTCAAGAGTTTCTTATGA

Coding sequence (CDS)

ATGTGGCCGTCGTTTCTCAGCCTCGTGTCCTTGTACGGCGGCTATATCCGCCGCTGCTTCACTTCCGCCGGCCTTTTCCAGCAAGTCATTGACATCGACACCGAAACCACCATCGCCTTTTGGGGCCCAAAGGCCAAAAAGTCCACCGCAAAACCCGCAGTCCTCCTCCTCCACGGCTTCGGCCCGATGGCGATTTGGCAATGGCGTCAACAGGTCCAATTCCTCGCCGATGATTTCGACGTCTACGTCCCCGACCTCGTCTTCTTCGGCGGCTCAACGACCAAATCGGCCGACCGGACGGAGGTTTTTCAGGCGATTTCGATCGGAAAGCTCATGGAGAAGATCGGAGTGGAAAAATACTCGGCGATGGGAACGAGCTACGGCGGATTTGTGGCGTATCATATGGCGAGGATTTGGCCGGAGAGGATCGAGAAGGTTATAATCGCGAGCTCCGGCGTTAATTTGAGACGGAAAGATAACGACGCGATGTTGAAAAGAGCTAACGTGGAGAAGATTGACGACTTTTTGCTGCCGGTGACGGCGGAACAATTACGGACGTTAATGAAGCTCGCCGTATTCAAGGGAGTGGGCAGAAGGATGCCGGACTTCTTCTTGAACGACTTTATCCATGCAGATGATGATGTTGATTTGTTTGGTGAACTTAGAATTGCATCTAAGAAATTATACATGGAAAACAGGGAGGAAAAGATTGAACTCCTAAAGTCGCTCACACTTGGAAAAGAAGAGACCATCAACCTCTTTCCACTTTCTCAGGAGGTATTGATAATATGGGGAGATCATGACCAGCTATTTCCCTTGGAAATGGCAAAAGAGCTCAAAGAGATTCTTGGGGAGAAGACAAGGCTGGAGGTGATGAAGGAGACCTCACACGTGCCTCAGATGGAAGCTCCTGCAGAATTCAATCGTCTTGTCAAGAGTTTCTTATGA

Protein sequence

MWPSFLSLVSLYGGYIRRCFTSAGLFQQVIDIDTETTIAFWGPKAKKSTAKPAVLLLHGFGPMAIWQWRQQVQFLADDFDVYVPDLVFFGGSTTKSADRTEVFQAISIGKLMEKIGVEKYSAMGTSYGGFVAYHMARIWPERIEKVIIASSGVNLRRKDNDAMLKRANVEKIDDFLLPVTAEQLRTLMKLAVFKGVGRRMPDFFLNDFIHADDDVDLFGELRIASKKLYMENREEKIELLKSLTLGKEETINLFPLSQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQMEAPAEFNRLVKSFL
Homology
BLAST of Spg025369 vs. NCBI nr
Match: XP_038885739.1 (monoacylglycerol lipase ABHD6-like isoform X1 [Benincasa hispida])

HSP 1 Score: 540.8 bits (1392), Expect = 7.8e-150
Identity = 267/315 (84.76%), Postives = 289/315 (91.75%), Query Frame = 0

Query: 1   MWPSFLSLVSLYGGYIRRCFTSAGLFQQVIDIDTETTIAFWGPKAKKSTAKPAVLLLHGF 60
           MWPSFLSLVS YGGY+RRCF++AGL  QVI+ID +TTIAFWGPK  KSTAKP++LLLHGF
Sbjct: 1   MWPSFLSLVSFYGGYLRRCFSAAGLSHQVINIDDQTTIAFWGPKPNKSTAKPSLLLLHGF 60

Query: 61  GPMAIWQWRQQVQFLADDFDVYVPDLVFFGGSTTKSADRTEVFQAISIGKLMEKIGVEKY 120
           GPMAIWQWRQQVQFLADDFDVYVPDLVFFGGSTTKSA+RTE+FQA+S+GKLMEKIGV+KY
Sbjct: 61  GPMAIWQWRQQVQFLADDFDVYVPDLVFFGGSTTKSAERTEIFQAMSVGKLMEKIGVKKY 120

Query: 121 SAMGTSYGGFVAYHMARIWPERIEKVIIASSGVNLRRKDNDAMLKRANVEKIDDFLLPVT 180
           S +GTSYGGFVAYHMARIWPERIEKVIIASSGVN+RRKDNDAMLKRANVEKID+FLLPVT
Sbjct: 121 SVLGTSYGGFVAYHMARIWPERIEKVIIASSGVNMRRKDNDAMLKRANVEKIDEFLLPVT 180

Query: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHADDDVDLFGELRIASKKLYMENREEKIELL 240
           AEQLRTLMKLAVFKGVGRRMPDFFLNDFIH                KLYME REEKIELL
Sbjct: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIH----------------KLYMEKREEKIELL 240

Query: 241 KSLTLGKEETINLFPLSQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQ 300
           KSLTLG+E+TI+L PL QEVLIIWGDHDQLFPLEMAKELKEI+GEKTRLEVMK+TSHVPQ
Sbjct: 241 KSLTLGREDTISLSPLFQEVLIIWGDHDQLFPLEMAKELKEIIGEKTRLEVMKDTSHVPQ 299

Query: 301 MEAPAEFNRLVKSFL 316
           +EAPAEFNRLVK+FL
Sbjct: 301 IEAPAEFNRLVKTFL 299

BLAST of Spg025369 vs. NCBI nr
Match: XP_023546569.1 (epoxide hydrolase 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 536.2 bits (1380), Expect = 1.9e-148
Identity = 266/315 (84.44%), Postives = 285/315 (90.48%), Query Frame = 0

Query: 1   MWPSFLSLVSLYGGYIRRCFTSAGLFQQVIDIDTETTIAFWGPKAKKSTAKPAVLLLHGF 60
           MWPSFLSLVSLY GY+RRCFT+AGL QQ IDID ETT++FWGPK  KS AKP++LLLHGF
Sbjct: 1   MWPSFLSLVSLYDGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKLSKSNAKPSLLLLHGF 60

Query: 61  GPMAIWQWRQQVQFLADDFDVYVPDLVFFGGSTTKSADRTEVFQAISIGKLMEKIGVEKY 120
           GPMAIWQWR QVQFLADDFDVYVPDLVFFGGSTTKSA+RTEVFQAISIGKL+EKIGV+KY
Sbjct: 61  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGGSTTKSAERTEVFQAISIGKLLEKIGVKKY 120

Query: 121 SAMGTSYGGFVAYHMARIWPERIEKVIIASSGVNLRRKDNDAMLKRANVEKIDDFLLPVT 180
           S MGTSYGGFVAYHMARIWPERIEKV+IASSGVN+RRKDNDAMLKRANVE ID+FLLP+T
Sbjct: 121 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 180

Query: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHADDDVDLFGELRIASKKLYMENREEKIELL 240
           AEQLRTLMKLAVFKGVGRRMPDFFLNDFIH                KLYME R+EKIELL
Sbjct: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIH----------------KLYMEKRKEKIELL 240

Query: 241 KSLTLGKEETINLFPLSQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQ 300
           KSLTLG+E+TI+L PL QEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQ
Sbjct: 241 KSLTLGREDTIDLSPLFQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQ 299

Query: 301 MEAPAEFNRLVKSFL 316
           +EAPAEFNR+ KSFL
Sbjct: 301 IEAPAEFNRVAKSFL 299

BLAST of Spg025369 vs. NCBI nr
Match: KAG6598595.1 (Epoxide hydrolase 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 536.2 bits (1380), Expect = 1.9e-148
Identity = 266/315 (84.44%), Postives = 286/315 (90.79%), Query Frame = 0

Query: 1   MWPSFLSLVSLYGGYIRRCFTSAGLFQQVIDIDTETTIAFWGPKAKKSTAKPAVLLLHGF 60
           MWPSF+SLVSLYGGY+RRCFTSAGL QQ I+ID ETT++FWGPK  KS AKP++LLLHGF
Sbjct: 1   MWPSFVSLVSLYGGYLRRCFTSAGLTQQAIEIDAETTLSFWGPKPSKSNAKPSLLLLHGF 60

Query: 61  GPMAIWQWRQQVQFLADDFDVYVPDLVFFGGSTTKSADRTEVFQAISIGKLMEKIGVEKY 120
           GPMAIWQWR QVQFLADDFDVYVPDLVFFG STTKSA+RTEVFQAISIGKL+EKIGV+KY
Sbjct: 61  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGDSTTKSAERTEVFQAISIGKLLEKIGVKKY 120

Query: 121 SAMGTSYGGFVAYHMARIWPERIEKVIIASSGVNLRRKDNDAMLKRANVEKIDDFLLPVT 180
           S MGTSYGGFVAYHMARIWPERIEKV+IASSGVN+RRKDNDAMLKRANVE ID+FLLP+T
Sbjct: 121 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 180

Query: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHADDDVDLFGELRIASKKLYMENREEKIELL 240
           AEQLRTLMKLAVFKGVGRRMPDFFLNDFIH                KLYME R+EKIELL
Sbjct: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIH----------------KLYMEKRKEKIELL 240

Query: 241 KSLTLGKEETINLFPLSQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQ 300
           KSLTLG+E+TI+L PL QEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQ
Sbjct: 241 KSLTLGREDTIDLSPLFQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQ 299

Query: 301 MEAPAEFNRLVKSFL 316
           +EAPAEFNR+VKSFL
Sbjct: 301 IEAPAEFNRVVKSFL 299

BLAST of Spg025369 vs. NCBI nr
Match: XP_022962163.1 (epoxide hydrolase 4-like [Cucurbita moschata])

HSP 1 Score: 536.2 bits (1380), Expect = 1.9e-148
Identity = 266/315 (84.44%), Postives = 285/315 (90.48%), Query Frame = 0

Query: 1   MWPSFLSLVSLYGGYIRRCFTSAGLFQQVIDIDTETTIAFWGPKAKKSTAKPAVLLLHGF 60
           MWPSFLSLVSLYGGY+RRCFT+AGL QQ IDID ETT++FWGPK  KS AKP++LLLHGF
Sbjct: 28  MWPSFLSLVSLYGGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKPSKSNAKPSLLLLHGF 87

Query: 61  GPMAIWQWRQQVQFLADDFDVYVPDLVFFGGSTTKSADRTEVFQAISIGKLMEKIGVEKY 120
           GPMAIWQWR QVQFLADDFDVYVPDLVFFG STTKSA+RTEVFQAISIGKL+EKIGV+KY
Sbjct: 88  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGDSTTKSAERTEVFQAISIGKLLEKIGVKKY 147

Query: 121 SAMGTSYGGFVAYHMARIWPERIEKVIIASSGVNLRRKDNDAMLKRANVEKIDDFLLPVT 180
           S MGTSYGGFVAYHMARIWPERIEKV+IASSGVN+RRKDNDAMLKRANVE ID+FLLP+T
Sbjct: 148 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 207

Query: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHADDDVDLFGELRIASKKLYMENREEKIELL 240
           AEQLRTLMKLAVFKGVGRRMPDFFLNDFIH                KLYME R+EKIELL
Sbjct: 208 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIH----------------KLYMEKRKEKIELL 267

Query: 241 KSLTLGKEETINLFPLSQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQ 300
           KSLT G+E+TI+L PL QEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQ
Sbjct: 268 KSLTFGREDTIDLSPLFQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQ 326

Query: 301 MEAPAEFNRLVKSFL 316
           +EAPAEFNR+VKSFL
Sbjct: 328 IEAPAEFNRVVKSFL 326

BLAST of Spg025369 vs. NCBI nr
Match: XP_022997555.1 (uncharacterized protein LOC111492447 [Cucurbita maxima])

HSP 1 Score: 533.1 bits (1372), Expect = 1.6e-147
Identity = 264/315 (83.81%), Postives = 284/315 (90.16%), Query Frame = 0

Query: 1   MWPSFLSLVSLYGGYIRRCFTSAGLFQQVIDIDTETTIAFWGPKAKKSTAKPAVLLLHGF 60
           MWPSFLSLVSLYGGY+RRCFT+AGL QQ IDID ETT++FWGPK  KS AKP++LLLHGF
Sbjct: 1   MWPSFLSLVSLYGGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKPSKSNAKPSLLLLHGF 60

Query: 61  GPMAIWQWRQQVQFLADDFDVYVPDLVFFGGSTTKSADRTEVFQAISIGKLMEKIGVEKY 120
           GPMAIWQWR QVQFLADDFDVYVPDLVFFGGS TKSA+RTEVFQAISIGKL+EKIGV+KY
Sbjct: 61  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGGSMTKSAERTEVFQAISIGKLLEKIGVKKY 120

Query: 121 SAMGTSYGGFVAYHMARIWPERIEKVIIASSGVNLRRKDNDAMLKRANVEKIDDFLLPVT 180
           S MGTSYGGFVAYHMARIWPERIEKV+IASSGVN+RRKDNDAMLKRANVE ID+FLLP+T
Sbjct: 121 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 180

Query: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHADDDVDLFGELRIASKKLYMENREEKIELL 240
           AEQLRTLMKLAVFKGVGRRMPDFFLNDFIH                KLYME R+EKIELL
Sbjct: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIH----------------KLYMEKRKEKIELL 240

Query: 241 KSLTLGKEETINLFPLSQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQ 300
           KSLTLG+E+TI+L PL QEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVP 
Sbjct: 241 KSLTLGREDTIDLSPLFQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPH 299

Query: 301 MEAPAEFNRLVKSFL 316
           +E+PAEFNR+VK FL
Sbjct: 301 IESPAEFNRVVKRFL 299

BLAST of Spg025369 vs. ExPASy Swiss-Prot
Match: Q6IE26 (Epoxide hydrolase 4 OS=Mus musculus OX=10090 GN=Ephx4 PE=2 SV=2)

HSP 1 Score: 62.8 bits (151), Expect = 8.1e-09
Identity = 63/278 (22.66%), Postives = 119/278 (42.81%), Query Frame = 0

Query: 51  KPAVLLLHGFGPMAIWQWRQQVQFLADDFDVYVPDLVFFGGSTTKSADRTEVFQAI--SI 110
           KP +LLLHGF P   + WR Q++    ++ V   DL  +G S   +   +     +   I
Sbjct: 91  KPLMLLLHGF-PEFWYSWRHQLREFKSEYRVVALDLRGYGESDAPAHQESYKLDCLIADI 150

Query: 111 GKLMEKIGVEKYSAMGTSYGGFVAYHMARIWPERIEKVIIAS----SGVNLRRKDNDAML 170
             +++ +G  K   +G  +GG +A+ +A  +PE I K+I+ +    S        + A L
Sbjct: 151 KDILDSLGYSKCVLIGHDWGGMIAWLIAVCYPEMIMKLIVINFPHPSVFTEYILRHPAQL 210

Query: 171 KRAN------VEKIDDFLLPVTAEQLRTLMKLAVFKGVGRRMPDFFLNDFIHADDDVDLF 230
            R++      + +  +F+  +   +    +  +   G+GR+       D    +  V +F
Sbjct: 211 FRSSFYYFFQIPRFPEFMFSINDFKALKHLFTSQSTGIGRKGRQLTTEDL---EAYVYVF 270

Query: 231 GELRIASKKL-YMENREEKIELLKSLTLGKEETINLFPLSQEVLIIWGDHDQLFPLEMAK 290
            +    S  + +  N    + L   +            ++   L++WG+ D    +EMA+
Sbjct: 271 SQPGALSGPINHYRNIFSCLPLKHHM------------VTTPTLLLWGEEDAFMEVEMAE 330

Query: 291 ELKEILGEKTRLEVMKETSHVPQMEAPAEFNRLVKSFL 316
             K  +    RL ++ E SH  Q + P   N L+ +FL
Sbjct: 331 VTKIYVKNYFRLTILSEGSHWLQQDQPDIVNGLIWAFL 352

BLAST of Spg025369 vs. ExPASy Swiss-Prot
Match: Q9KWQ6 (4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase OS=Rhodococcus jostii (strain RHA1) OX=101510 GN=hsaD PE=1 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 1.4e-08
Identity = 68/270 (25.19%), Postives = 119/270 (44.07%), Query Frame = 0

Query: 54  VLLLHGFGPMA-IW-QWRQQVQFLADDFDVYVPDLVFFGGSTTKSADRTEVF--QAISIG 113
           ++LLHG GP A  W  + + +  LA+ F V   D   +G S  K  +  + F   A ++ 
Sbjct: 38  IVLLHGGGPGASSWSNFARNIPVLAEKFHVLAVDQPGYGLS-DKPTEHPQYFVHSASALK 97

Query: 114 KLMEKIGV-EKYSAMGTSYGGFVAYHMARIWPERIEKVIIASSG---VNLRRKDNDAMLK 173
            L++ +GV  +   +G S GG  A   A  +P+R  ++++   G   VNL   D      
Sbjct: 98  DLLDTLGVGGRVHLLGNSLGGGAAVRFALDYPDRAGRLVLMGPGGLSVNLFAPD-----P 157

Query: 174 RANVEKIDDFLLPVTAEQLRTLMKLAVFKGVGRRMPDFFLNDFIHADDDVDLFGELRIAS 233
              V+ +  F    T E L   +++ VF    + + D  +++   A    +     +   
Sbjct: 158 TEGVKNLGKFGYQPTRENLEAFLRIMVFD--QKLITDELIDERFAAASTPESLAAAKAMG 217

Query: 234 KKLYMENREEKIELLKSLTLGKEETINLFPLSQEVLIIWGDHDQLFPLEMAKELKEILGE 293
           K     + + ++ +L           + + L Q VL+IWG  D++ PL+ A    +++  
Sbjct: 218 KS--FSSADFELGMLWR---------DAYKLRQRVLLIWGREDRVNPLDGALVALKMI-P 277

Query: 294 KTRLEVMKETSHVPQMEAPAEFNRLVKSFL 316
           + +L V     H  Q+E   EFNRL   FL
Sbjct: 278 RAQLHVFGGCGHWAQLEKFDEFNRLATDFL 287

BLAST of Spg025369 vs. ExPASy Swiss-Prot
Match: Q0IIS3 (Epoxide hydrolase 3 OS=Xenopus tropicalis OX=8364 GN=ephx3 PE=2 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 3.1e-08
Identity = 64/275 (23.27%), Postives = 120/275 (43.64%), Query Frame = 0

Query: 52  PAVLLLHGFGPMAIWQWRQQVQFLADDFDVYVPDLVFFGGSTTKSADRTEVFQAI--SIG 111
           P +LLLHGF P   + WR Q+   ++ +     DL  FGGS   S       + +   + 
Sbjct: 98  PLMLLLHGF-PENWYSWRYQLDEFSNGYRTVAIDLRGFGGSDAPSRLEDYKMEILLQDLQ 157

Query: 112 KLMEKIGVEKYSAMGTSYGGFVAYHMARIWPERIEKVIIASSGVNLRRKDNDAMLKRA-- 171
            L+  +G  +   +G  +GG +A+  A    + +  +I+ ++        +D +L     
Sbjct: 158 DLIRGLGYSRCVLVGHDWGGTLAWTFAVRHRDMVTHLIVMNA--PHPSAFHDYVLSHPSQ 217

Query: 172 --NVEKIDDFLLPVTAEQLRTLMKLAVFKGVGRRMPDFFLNDFIHADDDVDLFGELRIAS 231
             +   +  F LP+  E    L+ L  F+ + +      L D  H   +V+   +L    
Sbjct: 218 LFSSRYVFLFQLPLIPE---ILLSLRDFEHIKKP-----LTDATHGIQNVEC--KLSKEE 277

Query: 232 KKLYMENREEKIELLKSLTLGKEETINLFPL-SQEVLI----IWGDHDQLFPLEMAKELK 291
            + ++    +K  L   L   +      FP+ +Q+VL+    +WG+HD      M  E++
Sbjct: 278 VEAFVYYPSQKGALTPPLNYYR-NLFGFFPVKAQDVLVPTLLLWGEHDAFLEAAMVPEMQ 337

Query: 292 EILGEKTRLEVMKETSHVPQMEAPAEFNRLVKSFL 316
           + +    R E++   SH  Q + P E N++++ FL
Sbjct: 338 QYVRAPFRAEIIPNASHWLQQDRPQEVNKIIRDFL 358

BLAST of Spg025369 vs. ExPASy Swiss-Prot
Match: P9WNH4 (4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=hsaD PE=3 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 3.1e-08
Identity = 70/269 (26.02%), Postives = 118/269 (43.87%), Query Frame = 0

Query: 54  VLLLHGFGP-MAIW-QWRQQVQFLADDFDVYVPDLVFFGGSTTKSA-DRTEVFQAISIGK 113
           V+LLHG GP  A W  + + +  LA  F V   D   +G S  ++   +   + A+++  
Sbjct: 39  VVLLHGGGPGAASWTNFSRNIAVLARHFHVLAVDQPGYGHSDKRAEHGQFNRYAAMALKG 98

Query: 114 LMEKIGVEKYSAMGTSYGGFVAYHMARIWPERIEKVIIASSG---VNLRRKDNDAMLKRA 173
           L +++G+ +   +G S GG  A   A  +P R  ++++   G   +NL   D        
Sbjct: 99  LFDQLGLGRVPLVGNSLGGGTAVRFALDYPARAGRLVLMGPGGLSINLFAPD-----PTE 158

Query: 174 NVEKIDDFLLPVTAEQLRTLMKLAVFKGVGRRMPDFFLNDFIHADDDVDLFGELRIASKK 233
            V+++  F +  T E L   +++ V+       P+     F  A     L    R   K 
Sbjct: 159 GVKRLSKFSVAPTRENLEAFLRVMVY-DKNLITPELVDQRFALASTPESLTA-TRAMGKS 218

Query: 234 LYMENREEKIELLKSLTLGKEETINLFPLSQEVLIIWGDHDQLFPLEMA-KELKEILGEK 293
               + E  +       + +E    ++ L Q VL+IWG  D++ PL+ A   LK I   +
Sbjct: 219 FAGADFEAGM-------MWRE----VYRLRQPVLLIWGREDRVNPLDGALVALKTI--PR 278

Query: 294 TRLEVMKETSHVPQMEAPAEFNRLVKSFL 316
            +L V  +  H  Q+E   EFN+L   FL
Sbjct: 279 AQLHVFGQCGHWVQVEKFDEFNKLTIEFL 287

BLAST of Spg025369 vs. ExPASy Swiss-Prot
Match: P9WNH5 (4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=hsaD PE=1 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 3.1e-08
Identity = 70/269 (26.02%), Postives = 118/269 (43.87%), Query Frame = 0

Query: 54  VLLLHGFGP-MAIW-QWRQQVQFLADDFDVYVPDLVFFGGSTTKSA-DRTEVFQAISIGK 113
           V+LLHG GP  A W  + + +  LA  F V   D   +G S  ++   +   + A+++  
Sbjct: 39  VVLLHGGGPGAASWTNFSRNIAVLARHFHVLAVDQPGYGHSDKRAEHGQFNRYAAMALKG 98

Query: 114 LMEKIGVEKYSAMGTSYGGFVAYHMARIWPERIEKVIIASSG---VNLRRKDNDAMLKRA 173
           L +++G+ +   +G S GG  A   A  +P R  ++++   G   +NL   D        
Sbjct: 99  LFDQLGLGRVPLVGNSLGGGTAVRFALDYPARAGRLVLMGPGGLSINLFAPD-----PTE 158

Query: 174 NVEKIDDFLLPVTAEQLRTLMKLAVFKGVGRRMPDFFLNDFIHADDDVDLFGELRIASKK 233
            V+++  F +  T E L   +++ V+       P+     F  A     L    R   K 
Sbjct: 159 GVKRLSKFSVAPTRENLEAFLRVMVY-DKNLITPELVDQRFALASTPESLTA-TRAMGKS 218

Query: 234 LYMENREEKIELLKSLTLGKEETINLFPLSQEVLIIWGDHDQLFPLEMA-KELKEILGEK 293
               + E  +       + +E    ++ L Q VL+IWG  D++ PL+ A   LK I   +
Sbjct: 219 FAGADFEAGM-------MWRE----VYRLRQPVLLIWGREDRVNPLDGALVALKTI--PR 278

Query: 294 TRLEVMKETSHVPQMEAPAEFNRLVKSFL 316
            +L V  +  H  Q+E   EFN+L   FL
Sbjct: 279 AQLHVFGQCGHWVQVEKFDEFNKLTIEFL 287

BLAST of Spg025369 vs. ExPASy TrEMBL
Match: A0A6J1HE07 (epoxide hydrolase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111462697 PE=4 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 9.3e-149
Identity = 266/315 (84.44%), Postives = 285/315 (90.48%), Query Frame = 0

Query: 1   MWPSFLSLVSLYGGYIRRCFTSAGLFQQVIDIDTETTIAFWGPKAKKSTAKPAVLLLHGF 60
           MWPSFLSLVSLYGGY+RRCFT+AGL QQ IDID ETT++FWGPK  KS AKP++LLLHGF
Sbjct: 28  MWPSFLSLVSLYGGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKPSKSNAKPSLLLLHGF 87

Query: 61  GPMAIWQWRQQVQFLADDFDVYVPDLVFFGGSTTKSADRTEVFQAISIGKLMEKIGVEKY 120
           GPMAIWQWR QVQFLADDFDVYVPDLVFFG STTKSA+RTEVFQAISIGKL+EKIGV+KY
Sbjct: 88  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGDSTTKSAERTEVFQAISIGKLLEKIGVKKY 147

Query: 121 SAMGTSYGGFVAYHMARIWPERIEKVIIASSGVNLRRKDNDAMLKRANVEKIDDFLLPVT 180
           S MGTSYGGFVAYHMARIWPERIEKV+IASSGVN+RRKDNDAMLKRANVE ID+FLLP+T
Sbjct: 148 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 207

Query: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHADDDVDLFGELRIASKKLYMENREEKIELL 240
           AEQLRTLMKLAVFKGVGRRMPDFFLNDFIH                KLYME R+EKIELL
Sbjct: 208 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIH----------------KLYMEKRKEKIELL 267

Query: 241 KSLTLGKEETINLFPLSQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQ 300
           KSLT G+E+TI+L PL QEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQ
Sbjct: 268 KSLTFGREDTIDLSPLFQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQ 326

Query: 301 MEAPAEFNRLVKSFL 316
           +EAPAEFNR+VKSFL
Sbjct: 328 IEAPAEFNRVVKSFL 326

BLAST of Spg025369 vs. ExPASy TrEMBL
Match: A0A6J1KA03 (uncharacterized protein LOC111492447 OS=Cucurbita maxima OX=3661 GN=LOC111492447 PE=4 SV=1)

HSP 1 Score: 533.1 bits (1372), Expect = 7.9e-148
Identity = 264/315 (83.81%), Postives = 284/315 (90.16%), Query Frame = 0

Query: 1   MWPSFLSLVSLYGGYIRRCFTSAGLFQQVIDIDTETTIAFWGPKAKKSTAKPAVLLLHGF 60
           MWPSFLSLVSLYGGY+RRCFT+AGL QQ IDID ETT++FWGPK  KS AKP++LLLHGF
Sbjct: 1   MWPSFLSLVSLYGGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKPSKSNAKPSLLLLHGF 60

Query: 61  GPMAIWQWRQQVQFLADDFDVYVPDLVFFGGSTTKSADRTEVFQAISIGKLMEKIGVEKY 120
           GPMAIWQWR QVQFLADDFDVYVPDLVFFGGS TKSA+RTEVFQAISIGKL+EKIGV+KY
Sbjct: 61  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGGSMTKSAERTEVFQAISIGKLLEKIGVKKY 120

Query: 121 SAMGTSYGGFVAYHMARIWPERIEKVIIASSGVNLRRKDNDAMLKRANVEKIDDFLLPVT 180
           S MGTSYGGFVAYHMARIWPERIEKV+IASSGVN+RRKDNDAMLKRANVE ID+FLLP+T
Sbjct: 121 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 180

Query: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHADDDVDLFGELRIASKKLYMENREEKIELL 240
           AEQLRTLMKLAVFKGVGRRMPDFFLNDFIH                KLYME R+EKIELL
Sbjct: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIH----------------KLYMEKRKEKIELL 240

Query: 241 KSLTLGKEETINLFPLSQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQ 300
           KSLTLG+E+TI+L PL QEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVP 
Sbjct: 241 KSLTLGREDTIDLSPLFQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPH 299

Query: 301 MEAPAEFNRLVKSFL 316
           +E+PAEFNR+VK FL
Sbjct: 301 IESPAEFNRVVKRFL 299

BLAST of Spg025369 vs. ExPASy TrEMBL
Match: A0A6J1BQG4 (uncharacterized protein LOC111004859 OS=Momordica charantia OX=3673 GN=LOC111004859 PE=4 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 2.5e-146
Identity = 262/317 (82.65%), Postives = 286/317 (90.22%), Query Frame = 0

Query: 1   MWPSFLSLVSLYGGYIRRCFTSAGLFQQVIDIDTETTIAFWGPK--AKKSTAKPAVLLLH 60
           MWPSFLSLVSLYGGY+RRCFT+AGL  Q ++ID ETTIAFWGPK    K T K +++LLH
Sbjct: 1   MWPSFLSLVSLYGGYLRRCFTAAGLSGQAVEIDNETTIAFWGPKIITNKPTGKRSLVLLH 60

Query: 61  GFGPMAIWQWRQQVQFLADDFDVYVPDLVFFGGSTTKSADRTEVFQAISIGKLMEKIGVE 120
           GFGPMAIWQWR+QVQFLADDFDVYVPDLVFFGGSTTKS++RTEVFQAIS+GKL+EKIGV+
Sbjct: 61  GFGPMAIWQWRKQVQFLADDFDVYVPDLVFFGGSTTKSSERTEVFQAISVGKLLEKIGVK 120

Query: 121 KYSAMGTSYGGFVAYHMARIWPERIEKVIIASSGVNLRRKDNDAMLKRANVEKIDDFLLP 180
           KYS MGTSYGGFVAYHMAR WPERIEKVIIASSGVN+RRKDN+AMLKRANVEKIDDFLLP
Sbjct: 121 KYSVMGTSYGGFVAYHMARFWPERIEKVIIASSGVNMRRKDNEAMLKRANVEKIDDFLLP 180

Query: 181 VTAEQLRTLMKLAVFKGVGRRMPDFFLNDFIHADDDVDLFGELRIASKKLYMENREEKIE 240
           VTAEQLRTLMKLAVFK VGRR+PDFFLNDFIH                KLYMENREEKIE
Sbjct: 181 VTAEQLRTLMKLAVFKEVGRRLPDFFLNDFIH----------------KLYMENREEKIE 240

Query: 241 LLKSLTLGKEETINLFPLSQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHV 300
           LLKSLT+G+E+T+NL PLS+EVLIIWGDHDQLFPL+MAKELKEILGEKTRLEVMKETSHV
Sbjct: 241 LLKSLTIGREDTLNLSPLSEEVLIIWGDHDQLFPLQMAKELKEILGEKTRLEVMKETSHV 300

Query: 301 PQMEAPAEFNRLVKSFL 316
           PQ+E+PAEFNRLVKSFL
Sbjct: 301 PQIESPAEFNRLVKSFL 301

BLAST of Spg025369 vs. ExPASy TrEMBL
Match: A0A5D3BNV7 (Monoacylglycerol lipase ABHD6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold187G00070 PE=4 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 1.3e-145
Identity = 260/315 (82.54%), Postives = 286/315 (90.79%), Query Frame = 0

Query: 1   MWPSFLSLVSLYGGYIRRCFTSAGLFQQVIDIDTETTIAFWGPKAKKSTAKPAVLLLHGF 60
           MWPSFLSLVSLYG Y+RRCF++AGL QQVI ID ETTI+FWGPK  KSTAKP++LLLHGF
Sbjct: 1   MWPSFLSLVSLYGRYLRRCFSAAGLSQQVIHIDDETTISFWGPKPHKSTAKPSLLLLHGF 60

Query: 61  GPMAIWQWRQQVQFLADDFDVYVPDLVFFGGSTTKSADRTEVFQAISIGKLMEKIGVEKY 120
           GP AIWQWRQQVQFLADDFD+YVPDLVFFGGSTTKSA+RTE+FQA+SIGKL+E IGV+KY
Sbjct: 61  GPAAIWQWRQQVQFLADDFDLYVPDLVFFGGSTTKSAERTEIFQAMSIGKLVEMIGVKKY 120

Query: 121 SAMGTSYGGFVAYHMARIWPERIEKVIIASSGVNLRRKDNDAMLKRANVEKIDDFLLPVT 180
           S +GTSYGGFVAYHMARIWPERIEKVIIASSGVN+RRKDN+AMLKRANVEKID+FLLPVT
Sbjct: 121 SVLGTSYGGFVAYHMARIWPERIEKVIIASSGVNMRRKDNEAMLKRANVEKIDEFLLPVT 180

Query: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHADDDVDLFGELRIASKKLYMENREEKIELL 240
           AEQLRTLMKLAVFKG GR+MPDFF NDFIH                KLYMENRE+KIELL
Sbjct: 181 AEQLRTLMKLAVFKGGGRKMPDFFFNDFIH----------------KLYMENREQKIELL 240

Query: 241 KSLTLGKEETINLFPLSQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQ 300
           KSLTLG+E++INL PLSQEVLIIWGDHDQLFPLEMAKELK I+GEKTRLEV+KETSHVPQ
Sbjct: 241 KSLTLGREDSINLSPLSQEVLIIWGDHDQLFPLEMAKELKGIIGEKTRLEVLKETSHVPQ 299

Query: 301 MEAPAEFNRLVKSFL 316
           +EAPA+FN+LVKSFL
Sbjct: 301 IEAPAQFNQLVKSFL 299

BLAST of Spg025369 vs. ExPASy TrEMBL
Match: A0A1S3BDU7 (monoacylglycerol lipase ABHD6-like OS=Cucumis melo OX=3656 GN=LOC103488527 PE=4 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 1.3e-145
Identity = 260/315 (82.54%), Postives = 286/315 (90.79%), Query Frame = 0

Query: 1   MWPSFLSLVSLYGGYIRRCFTSAGLFQQVIDIDTETTIAFWGPKAKKSTAKPAVLLLHGF 60
           MWPSFLSLVSLYG Y+RRCF++AGL QQVI ID ETTI+FWGPK  KSTAKP++LLLHGF
Sbjct: 1   MWPSFLSLVSLYGRYLRRCFSAAGLSQQVIHIDDETTISFWGPKPHKSTAKPSLLLLHGF 60

Query: 61  GPMAIWQWRQQVQFLADDFDVYVPDLVFFGGSTTKSADRTEVFQAISIGKLMEKIGVEKY 120
           GP AIWQWRQQVQFLADDFD+YVPDLVFFGGSTTKSA+RTE+FQA+SIGKL+E IGV+KY
Sbjct: 61  GPAAIWQWRQQVQFLADDFDLYVPDLVFFGGSTTKSAERTEIFQAMSIGKLVEMIGVKKY 120

Query: 121 SAMGTSYGGFVAYHMARIWPERIEKVIIASSGVNLRRKDNDAMLKRANVEKIDDFLLPVT 180
           S +GTSYGGFVAYHMARIWPERIEKVIIASSGVN+RRKDN+AMLKRANVEKID+FLLPVT
Sbjct: 121 SVLGTSYGGFVAYHMARIWPERIEKVIIASSGVNMRRKDNEAMLKRANVEKIDEFLLPVT 180

Query: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHADDDVDLFGELRIASKKLYMENREEKIELL 240
           AEQLRTLMKLAVFKG GR+MPDFF NDFIH                KLYMENRE+KIELL
Sbjct: 181 AEQLRTLMKLAVFKGGGRKMPDFFFNDFIH----------------KLYMENREQKIELL 240

Query: 241 KSLTLGKEETINLFPLSQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQ 300
           KSLTLG+E++INL PLSQEVLIIWGDHDQLFPLEMAKELK I+GEKTRLEV+KETSHVPQ
Sbjct: 241 KSLTLGREDSINLSPLSQEVLIIWGDHDQLFPLEMAKELKGIIGEKTRLEVLKETSHVPQ 299

Query: 301 MEAPAEFNRLVKSFL 316
           +EAPA+FN+LVKSFL
Sbjct: 301 IEAPAQFNQLVKSFL 299

BLAST of Spg025369 vs. TAIR 10
Match: AT4G33180.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 335.9 bits (860), Expect = 3.5e-92
Identity = 170/316 (53.80%), Postives = 221/316 (69.94%), Query Frame = 0

Query: 4   SFLSLVSLYGGYIRRCFTSAGLFQQVIDIDTETTIAFWGPKA---KKSTAKPAVLLLHGF 63
           S+LS   L  GY+RRC  +AGL  Q + ID+ETTI FWGP     +    +P +LLLHGF
Sbjct: 5   SYLSPTRLLEGYLRRCLRAAGLTSQTLSIDSETTIHFWGPPPLDHRSDDDRPVMLLLHGF 64

Query: 64  GPMAIWQWRQQVQ-FLADDFDVYVPDLVFFGGSTTKSADRTEVFQAISIGKLMEKIGVEK 123
           GP ++WQWR+Q+Q F    F VY PDLVFFG ST+ S +RTEVFQA  + KLM KIG+ K
Sbjct: 65  GPSSMWQWRRQMQAFSPSAFRVYSPDLVFFGDSTSSSTNRTEVFQAECMAKLMAKIGIGK 124

Query: 124 YSAMGTSYGGFVAYHMARIWPERIEKVIIASSGVNLRRKDNDAMLKRANVEKIDDFLLPV 183
           Y+  GTSYGGFVAYHMA++WPE++EKV+IASSG+N+R+ D +++L+R+N E I+  +LP 
Sbjct: 125 YNVAGTSYGGFVAYHMAKMWPEKVEKVVIASSGINMRKCDGESLLQRSNCECIEKVMLPS 184

Query: 184 TAEQLRTLMKLAVFKGVGRRMPDFFLNDFIHADDDVDLFGELRIASKKLYMENREEKIEL 243
           TA + RTLM LA    + R  PD   ND I+                 LY +NR+EKIEL
Sbjct: 185 TATEFRTLMALASSWRLVRMFPDALWNDVIN----------------NLYKKNRKEKIEL 244

Query: 244 LKSLTLGKEETINLFPLSQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVP 303
           LK +T G+ E +N+  LSQEVLI+WGD DQ+FP++MA ELKEILG+KT+LE++  TSHVP
Sbjct: 245 LKGVTFGRSENLNIDSLSQEVLIVWGDKDQIFPVKMAYELKEILGDKTKLEIIDNTSHVP 304

Query: 304 QMEAPAEFNRLVKSFL 316
           Q+E   EFN +V  FL
Sbjct: 305 QIECAQEFNNIVLRFL 304

BLAST of Spg025369 vs. TAIR 10
Match: AT5G21950.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 323.9 bits (829), Expect = 1.4e-88
Identity = 161/318 (50.63%), Postives = 225/318 (70.75%), Query Frame = 0

Query: 1   MWPSFLSLVSLYGGYIRRCFTSAGLFQQVIDIDTETTIAFWGP---KAKKSTAKPAVLLL 60
           M PSFLS+       +RR F+SAGL  Q + ID+ETTI FWGP    + ++T KP++LLL
Sbjct: 1   MAPSFLSVARFVEALLRRRFSSAGLSLQTLSIDSETTIQFWGPPPSSSSENTQKPSLLLL 60

Query: 61  HGFGPMAIWQWRQQVQFLADDFDVYVPDLVFFGGSTTKSADRTEVFQAISIGKLMEKIGV 120
           HGFGP A+WQW  QV+ L+  F +YVPDLVFFGGS++   +R+E+FQA+ +GKLMEK+ V
Sbjct: 61  HGFGPSAVWQWSHQVKPLSHFFRLYVPDLVFFGGSSSSGENRSEMFQALCMGKLMEKLEV 120

Query: 121 EKYSAMGTSYGGFVAYHMARIWPERIEKVIIASSGVNLRRKDNDAMLKRANVEKIDDFLL 180
           E++S +GTSYGGFVAY+MA+++PE++EKV++ASSGVNLRR DN+A + RA   +I + +L
Sbjct: 121 ERFSVVGTSYGGFVAYNMAKMFPEKVEKVVLASSGVNLRRSDNEAFIARAKCHRIKEVML 180

Query: 181 PVTAEQLRTLMKLAVFKGVGRRMPDFFLNDFIHADDDVDLFGELRIASKKLYMENREEKI 240
           P +A  LR    +   K +   +PDF LNDF                 +K+Y E REEK 
Sbjct: 181 PASATDLRRFSGMVSSKRLD-YVPDFVLNDF----------------CQKMYSEKREEKA 240

Query: 241 ELLKSLTLGKEETINLFPLSQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSH 300
           ELL+ L++GK++  N+ P+ Q+V++IWG+ DQ+FPL+MA +LKE+LG K  L+V+++TSH
Sbjct: 241 ELLEGLSIGKDDKTNVSPIQQDVMLIWGEQDQVFPLKMAHDLKEMLGTKATLKVIQKTSH 300

Query: 301 VPQMEAPAEFNRLVKSFL 316
           +PQ E   EFN  V SFL
Sbjct: 301 IPQTEKSKEFNGFVMSFL 301

BLAST of Spg025369 vs. TAIR 10
Match: AT1G78210.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 209.1 bits (531), Expect = 5.0e-54
Identity = 119/301 (39.53%), Postives = 182/301 (60.47%), Query Frame = 0

Query: 20  FTSAGLFQQVIDIDTETTIAFWGPKAK-KSTAKPAVLLLHGFGPMAIWQWRQQVQFLADD 79
           F  +GL    ID+   T + FW  K K +S  KP +LL+HG G  AIWQW    + L+  
Sbjct: 19  FKRSGLRPVTIDLKDGTVVNFWVSKTKPESKPKPNLLLIHGLGATAIWQWYDVARRLSRY 78

Query: 80  FDVYVPDLVFFGGSTTKSADRTEVFQAISIGKLMEKIGVEKYSAMGTSYGGFVAYHMARI 139
           F++Y+PDLVFFGGS+T   +R+++FQA ++ + +E   V+K+S +G SYGGFV Y MA +
Sbjct: 79  FNLYIPDLVFFGGSSTTRPERSDIFQAQTLMRALEAQSVKKFSLVGLSYGGFVGYRMASM 138

Query: 140 WPERIEKVIIASSGVNLRRKDNDA-MLKRANVEKIDDFLLPVTAEQLRTLMKLAVFK-GV 199
           + + +EKV+I  + V +  KD  A + K +++++    L+P + ++LR LM    +K  +
Sbjct: 139 YADAVEKVVICCAAVCVEEKDMKAGVFKVSDLDEASKILVPESVKKLRELMGYIFYKPAL 198

Query: 200 GRRMPDFFLNDFI-HADDDVDLFGELRIASKKLYMENREEKIELLKSLTLGKEETINLFP 259
            R +P   L+DFI HA                L  +N EEK EL+K++   K+  I+  P
Sbjct: 199 ARLVPTCLLHDFIEHA----------------LTRDNMEEKRELIKAIP--KDRIISEIP 258

Query: 260 -LSQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQMEAPAEFNRLVKSF 316
            L Q  LIIWG+HDQ+FPLEM K L++ +G+  +L ++K T H+   E P +F +L+KSF
Sbjct: 259 KLKQPTLIIWGEHDQVFPLEMGKRLEKHVGDNGKLVIIKRTGHIFNFEKPKKFIKLLKSF 301

BLAST of Spg025369 vs. TAIR 10
Match: AT5G09430.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 197.2 bits (500), Expect = 2.0e-50
Identity = 119/306 (38.89%), Postives = 180/306 (58.82%), Query Frame = 0

Query: 17  RRCFTSAGLFQQVIDIDTETTIA-----FWGPKAKKSTAKPAVLLLHGFGPMAIWQWRQQ 76
           R+ F +AGL     D+    +IA      W PK+  + +KP +LLLHGFG  A+WQ+ + 
Sbjct: 22  RQSFANAGLRSVTTDLSHGNSIASTAMHCWIPKS-PNRSKPNLLLLHGFGANAMWQYGEH 81

Query: 77  VQFLADDFDVYVPDLVFFGGSTTKSADRTEVFQAISIGKLMEKIGVEKYSAMGTSYGGFV 136
           ++     F+VYVPDL+FFG S+T   +RTE FQA  + +LME  GV++ + +G SYGGFV
Sbjct: 82  LRAFTGRFNVYVPDLLFFGLSSTSEPNRTESFQARCLMRLMEAHGVQRMNIVGISYGGFV 141

Query: 137 AYHMARIWPERIEKVIIASSGVNLRRKD-NDAMLKRANVEKIDDFLLPVTAEQLRTLMKL 196
            Y +A  +PE +EK+++  +GV L  KD  D + K  N+E+    L+P T E+L+ L++ 
Sbjct: 142 GYSLAAQFPENVEKLVLCCAGVCLEEKDMEDGLFKVPNLEEATGILIPQTPEKLKELIRF 201

Query: 197 AVFKGVGRRMPDFFLNDFIHADDDVDLFGELRIASKKLYMENREEKIELLKSLTLGKEET 256
           +  K + + +P FFL DFI    DV            +  E  EEK +L+KS+   K+  
Sbjct: 202 SFVKPI-KGVPSFFLWDFI----DV------------MCTEFVEEKRDLIKSIL--KDRR 261

Query: 257 INLFP-LSQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQMEAPAEFNR 316
           ++  P + Q+ LIIWG+ DQ+FPLE+   LK  +GE   + V+K+  H   +E   EF +
Sbjct: 262 LSDLPRIKQKSLIIWGEEDQIFPLELGYRLKRHIGESAEIVVIKKAGHAVNLEKSKEFVK 307

BLAST of Spg025369 vs. TAIR 10
Match: AT4G39955.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 183.7 bits (465), Expect = 2.2e-46
Identity = 116/314 (36.94%), Postives = 165/314 (52.55%), Query Frame = 0

Query: 4   SFLSLVSLYGGYIRRCFTSAGLFQQVIDIDTETTIAFWGPKAKKSTAKPAVLLLHGFGPM 63
           S  S VS      +  F+ AGL     D+   T    W P     T KP +LLLHG G  
Sbjct: 3   SCFSYVSSRNKCYQYSFSRAGLRSSTSDLGDGTVFHCWIPLTHIHT-KPTLLLLHGIGAN 62

Query: 64  AIWQWRQQVQFLADDFDVYVPDLVFFGGSTTKSADRTEVFQAISIGKLMEKIGVEKYSAM 123
           A+WQW + +      F+VYVPDL+FFG S T   DR+E FQA  + K M+  GV   +  
Sbjct: 63  AMWQWDRFIDRFIPRFNVYVPDLIFFGDSYTTRPDRSESFQATCVMKAMDAYGVRTMTVA 122

Query: 124 GTSYGGFVAYHMARIWPERIEKVIIASSGVNLRRKDN-DAMLKRANVEKIDDFLLPVTAE 183
           G SYGGFVAY +A  + ER+++V++  +GV L  KD+ D M K  + E+    L P +  
Sbjct: 123 GLSYGGFVAYSLAAQFKERVDRVVLICAGVALEEKDSEDGMFKVKSPEEAAAVLFPQSPS 182

Query: 184 QLRTLMKLAVFKGVGRRMPDFFLNDFIHADDDVDLFGELRIASKKLYMENREEKIELLKS 243
            LR L++L+ +K     +P  F  D+IH                K Y++ R+E +E L  
Sbjct: 183 MLRRLLQLSFYK-PPIWIPSCFAMDYIH-------------VMCKDYLQERKELVEALHK 242

Query: 244 LTLGKEETINLFPLSQEVLIIWGDHDQLFPLEMAKELKEILGE-KTRLEVMKETSHVPQM 303
                    NL  ++Q  L+IWG+ DQ+FP+E+A  LK  LGE + +L ++K+T H    
Sbjct: 243 ----GRRFANLPKITQPTLMIWGEEDQVFPVELAHRLKRYLGEDRAQLVLLKKTGHAINE 297

Query: 304 EAPAEFNRLVKSFL 316
           E P E  + +KSFL
Sbjct: 303 EKPKEMYKHMKSFL 297

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038885739.17.8e-15084.76monoacylglycerol lipase ABHD6-like isoform X1 [Benincasa hispida][more]
XP_023546569.11.9e-14884.44epoxide hydrolase 4-like [Cucurbita pepo subsp. pepo][more]
KAG6598595.11.9e-14884.44Epoxide hydrolase 4, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022962163.11.9e-14884.44epoxide hydrolase 4-like [Cucurbita moschata][more]
XP_022997555.11.6e-14783.81uncharacterized protein LOC111492447 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q6IE268.1e-0922.66Epoxide hydrolase 4 OS=Mus musculus OX=10090 GN=Ephx4 PE=2 SV=2[more]
Q9KWQ61.4e-0825.194,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase O... [more]
Q0IIS33.1e-0823.27Epoxide hydrolase 3 OS=Xenopus tropicalis OX=8364 GN=ephx3 PE=2 SV=1[more]
P9WNH43.1e-0826.024,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase O... [more]
P9WNH53.1e-0826.024,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase O... [more]
Match NameE-valueIdentityDescription
A0A6J1HE079.3e-14984.44epoxide hydrolase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111462697 PE=4 SV=1[more]
A0A6J1KA037.9e-14883.81uncharacterized protein LOC111492447 OS=Cucurbita maxima OX=3661 GN=LOC111492447... [more]
A0A6J1BQG42.5e-14682.65uncharacterized protein LOC111004859 OS=Momordica charantia OX=3673 GN=LOC111004... [more]
A0A5D3BNV71.3e-14582.54Monoacylglycerol lipase ABHD6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3BDU71.3e-14582.54monoacylglycerol lipase ABHD6-like OS=Cucumis melo OX=3656 GN=LOC103488527 PE=4 ... [more]
Match NameE-valueIdentityDescription
AT4G33180.13.5e-9253.80alpha/beta-Hydrolases superfamily protein [more]
AT5G21950.11.4e-8850.63alpha/beta-Hydrolases superfamily protein [more]
AT1G78210.15.0e-5439.53alpha/beta-Hydrolases superfamily protein [more]
AT5G09430.12.0e-5038.89alpha/beta-Hydrolases superfamily protein [more]
AT4G39955.12.2e-4636.94alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PRINTSPR00111ABHYDROLASEcoord: 78..93
score: 35.99
coord: 136..149
score: 30.54
coord: 122..135
score: 33.74
IPR000073Alpha/beta hydrolase fold-1PFAMPF12697Abhydrolase_6coord: 54..309
e-value: 2.9E-13
score: 51.0
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 21..315
e-value: 2.6E-43
score: 150.8
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 50..315
NoneNo IPR availablePANTHERPTHR43139SI:DKEY-122A22.2coord: 5..315
NoneNo IPR availablePANTHERPTHR43139:SF52ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 5..315

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg025369.1Spg025369.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity