Spg025357 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg025357 vs. NCBI nr
Match: XP_038885098.1 (subtilisin-like protease SBT1.7 [Benincasa hispida])

HSP 1 Score: 1395.6 bits (3611), Expect = 0.0e+00
Identity = 703/772 (91.06%), Postives = 733/772 (94.95%), Query Frame = 0

Query: 4   MKLQTCRLSLWFLLFLISFCFCSFSVALKKNQQLKKKTYIVHMDRTNMPLAFDDHFQWYD 63
           MK+QTCRLS WFLLFLISFC CSF+ A K N+QLKKKTY++HMDRTNMP AFDDHF+WYD
Sbjct: 1   MKMQTCRLSQWFLLFLISFCSCSFTEAQKSNRQLKKKTYVIHMDRTNMPQAFDDHFKWYD 60

Query: 64  SSLKSVSDSAQMLYSYNTVVHGFSTRLTVEEAKLIEKQLGILAVVPEEKYELHTTRTPEF 123
           SSLKSVS+SAQMLYSYNTVVHGFSTRLTVEEAKLIEKQ GILAV+PE KYELHTTRTPEF
Sbjct: 61  SSLKSVSNSAQMLYSYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPEVKYELHTTRTPEF 120

Query: 124 LGLGKSVSFLPASEKVGEVIIGVLDTGVWPELESFNDAGLGPVPASWKGECEVGKNFTSS 183
           LGLGKSVSF PASEKV EVIIGVLDTGVWPELESFNDAGLGPVPASWKGECEVGKNFTSS
Sbjct: 121 LGLGKSVSFFPASEKVSEVIIGVLDTGVWPELESFNDAGLGPVPASWKGECEVGKNFTSS 180

Query: 184 SCNRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGTHTSTTAAGSAVTGANLFGYA 243
           SCNRKLIGARYFSKGYEAAFG IDESQESKSPRDDDGHG+HTSTTAAGSAVTGA+LFG+A
Sbjct: 181 SCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFA 240

Query: 244 AGTARGMAAEARVATYKVCWLGGCFGSDILAAMDKAVGDGVNVLSLSLGGSSPDYYRDNV 303
           AGTARGMAAEARVATYKVCWLGGCF SDILAA+DKAV DGVNVLSLSLGGSSPDYYRDNV
Sbjct: 241 AGTARGMAAEARVATYKVCWLGGCFSSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNV 300

Query: 304 AIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKFT 363
           AIGAFSAAAQGVFVSCSAGNGGPSSG+LSNVAPWITTVGAGTLDRDFPAYVTLGNGKK T
Sbjct: 301 AIGAFSAAAQGVFVSCSAGNGGPSSGTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKIT 360

Query: 364 GESLYSGKPLPDSLIPIVYAGNASNSTSGSLCLTSTLIPAKVAGKIVVCDRGGNSRVQKG 423
           GESLYSGKPLPDSL+PIVYAGNASNSTSGSLCL+STL PAKVAGKIVVCDRGGNSRVQKG
Sbjct: 361 GESLYSGKPLPDSLLPIVYAGNASNSTSGSLCLSSTLNPAKVAGKIVVCDRGGNSRVQKG 420

Query: 424 VVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKAGDAIKSYISSDANPTATISTG 483
           +VVKEAGG GMILANTE YGEEQLADAHL P AAVG+K+GDAIKSYISSDANPTATISTG
Sbjct: 421 LVVKEAGGVGMILANTEAYGEEQLADAHLTPTAAVGKKSGDAIKSYISSDANPTATISTG 480

Query: 484 TTRLGVQPSPVVAAFSSRGPNFLTPQILKPDLIAPGVNILAGWTGGAGPTGSEDDKRRVP 543
           TTRLGVQPSPVVAAFSSRGPN LTP ILKPDLIAPGVNILAGWTGGAGPTG + DKR V 
Sbjct: 481 TTRLGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVA 540

Query: 544 FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTSYSTYKSGEAIQDISNGLAS 603
           FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTT+YSTYK+GE IQD+SNG  S
Sbjct: 541 FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGEVIQDVSNGSPS 600

Query: 604 TPFDIGAGHVNPTAALDPGLVYDTTTDDYFAFLCALNYSSLQIKVIAKKDFTCSANKNYR 663
           TPFDIGAGHVNPTAALDPGLVYDTTTDDYFAFLCALNYSSLQIKVI+K+DFTC+ NKNY+
Sbjct: 601 TPFDIGAGHVNPTAALDPGLVYDTTTDDYFAFLCALNYSSLQIKVISKRDFTCNGNKNYK 660

Query: 664 LEDLNYPSFAVPLETPSTRGGGDAAPTTVKYTRTLTNKGASSTYKATVTSKSPSVKIVVE 723
           LEDLNYPSFAVPLETPSTRGG D APTTVKYTRTLTNKGA STYK +VT+    VKIVVE
Sbjct: 661 LEDLNYPSFAVPLETPSTRGGEDVAPTTVKYTRTLTNKGAPSTYKVSVTAPK-EVKIVVE 720

Query: 724 PESLSFAEANEQKSYTVTFIAFPMPSGTVSFARLEWSDGKHVVGSPIAFTWT 776
           PESLSFAEANEQKSYTVTFIA PMPSG+ SFARLEWSDGKH+VGSPIAFTWT
Sbjct: 721 PESLSFAEANEQKSYTVTFIASPMPSGSESFARLEWSDGKHIVGSPIAFTWT 771

BLAST of Spg025357 vs. NCBI nr
Match: XP_022131596.1 (subtilisin-like protease SBT1.7 [Momordica charantia])

HSP 1 Score: 1389.4 bits (3595), Expect = 0.0e+00
Identity = 701/775 (90.45%), Postives = 734/775 (94.71%), Query Frame = 0

Query: 1   MKKMKLQTCRLSLWFLLFLISFCFCSFSVALKKNQQLKKKTYIVHMDRTNMPLAFDDHFQ 60
           MKKMK+QTCR+S  FLLFLI F   S+  A K NQ+LKKKTYI+HMDRTNMP AFDDHFQ
Sbjct: 1   MKKMKMQTCRVSRLFLLFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQ 60

Query: 61  WYDSSLKSVSDSAQMLYSYNTVVHGFSTRLTVEEAKLIEKQLGILAVVPEEKYELHTTRT 120
           WYDSSLKSVSDSAQMLYSY+TV+HGFSTRLTVEEA+L+EK+ GILAV+PE KYELHTTRT
Sbjct: 61  WYDSSLKSVSDSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRT 120

Query: 121 PEFLGLGKSVSFLPASEKVGEVIIGVLDTGVWPELESFNDAGLGPVPASWKGECEVGKNF 180
           PEFLGLGKS SF PAS KVGEVIIG+LDTGVWPELESFNDAGLGPVP SWKGECEVGKNF
Sbjct: 121 PEFLGLGKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNF 180

Query: 181 TSSSCNRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGTHTSTTAAGSAVTGANLF 240
           +SSSCNRKLIGARYF+KGYE+AFGPIDESQESKSP+DDDGHGTHTSTTAAGSAVTGANLF
Sbjct: 181 SSSSCNRKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLF 240

Query: 241 GYAAGTARGMAAEARVATYKVCWLGGCFGSDILAAMDKAVGDGVNVLSLSLGGSSPDYYR 300
           GYA+GTARGMAA+ARVATYKVCWLGGCFGSDILAA+DKAV DGVNVLSLSLGGSSPDYYR
Sbjct: 241 GYASGTARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYR 300

Query: 301 DNVAIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYVTLGNGK 360
           DNVAIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAY+TLGNGK
Sbjct: 301 DNVAIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGK 360

Query: 361 KFTGESLYSGKPLPDSLIPIVYAGNASNSTSGSLCLTSTLIPAKVAGKIVVCDRGGNSRV 420
           KFTGESLYSGKPLPDSL+PIVYA +ASNSTSGS CLT TL PAKVAGKIVVCDRGGNSRV
Sbjct: 361 KFTGESLYSGKPLPDSLVPIVYAAHASNSTSGSSCLTGTLNPAKVAGKIVVCDRGGNSRV 420

Query: 421 QKGVVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKAGDAIKSYISSDANPTATI 480
           QKGVVVKEAGGAGMILANTETYGEE+LADAHLLPAAAVGQK GDAIKSYISSDANPTA  
Sbjct: 421 QKGVVVKEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKF 480

Query: 481 STGTTRLGVQPSPVVAAFSSRGPNFLTPQILKPDLIAPGVNILAGWTGGAGPTGSEDDKR 540
           STGTTRLGVQPSPVVAAFSSRGPN LTP ILKPDLIAPGVNILAGWTG AGPTG + DKR
Sbjct: 481 STGTTRLGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKR 540

Query: 541 RVPFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTSYSTYKSGEAIQDISNG 600
           RV FNIISGTSMSCPHISGLAAL+KAAHPDWSPAAIRSALMTT+YSTYK+GEAIQDIS+G
Sbjct: 541 RVAFNIISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSG 600

Query: 601 LASTPFDIGAGHVNPTAALDPGLVYDTTTDDYFAFLCALNYSSLQIKVIAKKDFTCSANK 660
           L STPFDIGAGHVNP AALDPGLVYDTTTDDYF FLCALNY+SLQIKVIAKKDFTCSA+K
Sbjct: 601 LPSTPFDIGAGHVNPGAALDPGLVYDTTTDDYFVFLCALNYTSLQIKVIAKKDFTCSASK 660

Query: 661 NYRLEDLNYPSFAVPLETPSTRGGGDAAPTTVKYTRTLTNKGASSTYKATVTSKSPSVKI 720
           NY+LEDLNYPSFAVPLETPS RGGGDAAPTTVKYTRTLTNK ASSTYK + TSKSPSVKI
Sbjct: 661 NYKLEDLNYPSFAVPLETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVSETSKSPSVKI 720

Query: 721 VVEPESLSFAEANEQKSYTVTFIAFPMPSGTVSFARLEWSDGKHVVGSPIAFTWT 776
           VVEPESLSFAE NEQKSYTVTFIA PMPSGT SFARLEWSDGKH+VGSPIAFTWT
Sbjct: 721 VVEPESLSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFTWT 775

BLAST of Spg025357 vs. NCBI nr
Match: XP_004142884.1 (subtilisin-like protease SBT1.7 [Cucumis sativus] >KGN62460.1 hypothetical protein Csa_018794 [Cucumis sativus])

HSP 1 Score: 1375.5 bits (3559), Expect = 0.0e+00
Identity = 686/770 (89.09%), Postives = 727/770 (94.42%), Query Frame = 0

Query: 6   LQTCRLSLWFLLFLISFCFCSFSVALKKNQQLKKKTYIVHMDRTNMPLAFDDHFQWYDSS 65
           ++TCR+S WFLLFLISFC CSF+ A K NQQLKKKTYI+HMD+TNMP AFDDHFQWYDSS
Sbjct: 1   MKTCRVSQWFLLFLISFCSCSFTEAQKSNQQLKKKTYIIHMDKTNMPQAFDDHFQWYDSS 60

Query: 66  LKSVSDSAQMLYSYNTVVHGFSTRLTVEEAKLIEKQLGILAVVPEEKYELHTTRTPEFLG 125
           LKSVSDSAQMLYSYNTV+HGFSTRLTVEEAKL+EKQ GI+AV+PE KYELHTTRTPEFLG
Sbjct: 61  LKSVSDSAQMLYSYNTVIHGFSTRLTVEEAKLMEKQEGIIAVIPEMKYELHTTRTPEFLG 120

Query: 126 LGKSVSFLPASEKVGEVIIGVLDTGVWPELESFNDAGLGPVPASWKGECEVGKNFTSSSC 185
           LGKSVSF PASEKV EVIIGVLDTGVWPELESF+DAGLGP+PASWKGECEVGKNFTSS+C
Sbjct: 121 LGKSVSFFPASEKVSEVIIGVLDTGVWPELESFSDAGLGPIPASWKGECEVGKNFTSSNC 180

Query: 186 NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGTHTSTTAAGSAVTGANLFGYAAG 245
           NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHG+HTSTTAAGSAVTGANLFG+AAG
Sbjct: 181 NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAG 240

Query: 246 TARGMAAEARVATYKVCWLGGCFGSDILAAMDKAVGDGVNVLSLSLGGSSPDYYRDNVAI 305
           TARGMAAEARVATYKVCWLGGCF SDILAAMDK+V DG N+LS+SLGG+S DYYRDNVAI
Sbjct: 241 TARGMAAEARVATYKVCWLGGCFSSDILAAMDKSVEDGCNILSVSLGGNSADYYRDNVAI 300

Query: 306 GAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKFTGE 365
           GAFSA AQGVFVSCSAGNGGPSS +LSNVAPWITTVGAGTLDRDFPAYVTLGNGKK TGE
Sbjct: 301 GAFSATAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGE 360

Query: 366 SLYSGKPLPDSLIPIVYAGNASNSTSGSLCLTSTLIPAKVAGKIVVCDRGGNSRVQKGVV 425
           SLYSGKPLP+SL+PIV A +ASNS+SGSLCL+ TL PAKV GKIVVCDRGGNSRVQKGVV
Sbjct: 361 SLYSGKPLPNSLLPIVSAASASNSSSGSLCLSGTLNPAKVTGKIVVCDRGGNSRVQKGVV 420

Query: 426 VKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKAGDAIKSYISSDANPTATISTGTT 485
           VKEAGG GMILANTE YGEEQLADAHL+P AAVGQKAGDAIK+YISSD+NPTATISTGTT
Sbjct: 421 VKEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQKAGDAIKNYISSDSNPTATISTGTT 480

Query: 486 RLGVQPSPVVAAFSSRGPNFLTPQILKPDLIAPGVNILAGWTGGAGPTGSEDDKRRVPFN 545
           RLGVQPSPVVAAFSSRGPN LTPQILKPDLIAPGVNILAGWTGGAGPTG + DKR V FN
Sbjct: 481 RLGVQPSPVVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFN 540

Query: 546 IISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTSYSTYKSGEAIQDISNGLASTP 605
           IISGTSMSCPHISGLAAL+KAAHPDWSPAAIRSALMTT+YSTYK+GE IQDISNG  STP
Sbjct: 541 IISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEMIQDISNGSPSTP 600

Query: 606 FDIGAGHVNPTAALDPGLVYDTTTDDYFAFLCALNYSSLQIKVIAKKDFTCSANKNYRLE 665
           FDIGAGHVNPTAALDPGLVYDTTTDDY AFLCALNYSSLQIKVI+KKDFTC+ NKNY+LE
Sbjct: 601 FDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLE 660

Query: 666 DLNYPSFAVPLETPSTRGGGDAAPTTVKYTRTLTNKGASSTYKATVTSKSPSVKIVVEPE 725
           DLNYPSFAVPLETPSTRGG + APTT+KYTRTLTNKGASSTYK +VT+KS SVKIVVEPE
Sbjct: 661 DLNYPSFAVPLETPSTRGGENVAPTTIKYTRTLTNKGASSTYKVSVTAKSSSVKIVVEPE 720

Query: 726 SLSFAEANEQKSYTVTFIAFPMPSGTVSFARLEWSDGKHVVGSPIAFTWT 776
           SLSF E NEQKSYTVTFIA PMPSG+ SFARLEWSDGKH+VGSPIAFTWT
Sbjct: 721 SLSFTEVNEQKSYTVTFIASPMPSGSQSFARLEWSDGKHIVGSPIAFTWT 770

BLAST of Spg025357 vs. NCBI nr
Match: KAG7029314.1 (Subtilisin-like protease SBT1.7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1362.8 bits (3526), Expect = 0.0e+00
Identity = 688/773 (89.00%), Postives = 725/773 (93.79%), Query Frame = 0

Query: 4   MKLQTCRLSLWFLLFLISFCFCSFSVALKKNQQLKKKTYIVHMDRTNMPLAFDDHFQWYD 63
           MK+QTCR+S WFLLFLIS C CSF+ A K +QQLKKKTYI+HMDRTNMP AFDDHFQWYD
Sbjct: 1   MKIQTCRVSQWFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYD 60

Query: 64  SSLKSVSDSAQMLYSYNTVVHGFSTRLTVEEAKLIEKQLGILAVVPEEKYELHTTRTPEF 123
           SSLKSVSDSAQ LY YNTVVHGFSTRLTVEEAKLIEKQ GILAV+PE KY+LHTTRTPEF
Sbjct: 61  SSLKSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEF 120

Query: 124 LGLGKSVSFLPASEKVGEVIIGVLDTGVWPELESFNDAGLGPVPASWKGECEVGKNFTSS 183
           LGL KSVSF PAS KVGEVI+GVLDTGV PELESF+D GLGPVP SWKGECEVGKNF+SS
Sbjct: 121 LGLEKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSS 180

Query: 184 SCNRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGTHTSTTAAGSAVTGANLFGYA 243
           SCNRKLIGARYFSKGYEAAFG IDESQESKSPRDDDGHG+HTSTTAAGSAVTGA+LFG+A
Sbjct: 181 SCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFA 240

Query: 244 AGTARGMAAEARVATYKVCWLGGCFGSDILAAMDKAVGDGVNVLSLSLGGSSPDYYRDNV 303
           AGTA+GMAAEARVATYKVCWLGGCFGSDILAAMDKA+ DGVNVLSLSLGGSSPDYYRDNV
Sbjct: 241 AGTAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNV 300

Query: 304 AIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKFT 363
           AIGAFSA AQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFP YVTLGNGKKFT
Sbjct: 301 AIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFT 360

Query: 364 GESLYSGKPLPDSLIPIVYAGNASNSTSGSLCLTSTLIPAKVAGKIVVCDRGGNSRVQKG 423
           G+SLY+GKPL DSLIPIVYA +ASNSTSGSLCLTSTL PAKVAGKIVVCDRGGNSRVQKG
Sbjct: 361 GQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKG 420

Query: 424 VVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKAGDAIKSYISSDANPTATISTG 483
           +VVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQK GDAIKSYIS++ANPTATIS G
Sbjct: 421 LVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISAG 480

Query: 484 TTRLGVQPSPVVAAFSSRGPNFLTPQILKPDLIAPGVNILAGWTGGAGPTGSEDDKRRVP 543
           TTRLGVQPSP+VAAFSSRGPN LTPQILKPDLIAPGVNILAGW GG GPTG + DKR V 
Sbjct: 481 TTRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWAGGVGPTGLDSDKRHVA 540

Query: 544 FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTSYSTYKSGEAIQDISNGLAS 603
           FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTT+YSTYK+GEAIQD+S+GL S
Sbjct: 541 FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLPS 600

Query: 604 TPFDIGAGHVNPTAALDPGLVYDTTTDDYFAFLCALNYSSLQIKVIAKKDFTCSANKNYR 663
           TPFDIGAGHVNPTAALDPGLVYD T +DYFAFLCALNYSS QIKVI+KKDFTCS NKNY+
Sbjct: 601 TPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKNYK 660

Query: 664 LEDLNYPSFAVPLETPSTRGGGD-AAPTTVKYTRTLTNKGASSTYKATVTSKSPSVKIVV 723
           LEDLNYPSFAV LETPST+GGG+  APTTVKYTRTLTNKGA STYK +VTSKSPSVKI+V
Sbjct: 661 LEDLNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIMV 720

Query: 724 EPESLSFAEANEQKSYTVTFIAFPMPSGTVSFARLEWSDGKHVVGSPIAFTWT 776
           EPESLSFA+ NEQKSYTVTF+A  MPSG+ SFARLEWSDGKH VGSPIAFTWT
Sbjct: 721 EPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT 773

BLAST of Spg025357 vs. NCBI nr
Match: KAG6598344.1 (Subtilisin-like protease 1.7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1362.4 bits (3525), Expect = 0.0e+00
Identity = 688/773 (89.00%), Postives = 725/773 (93.79%), Query Frame = 0

Query: 4   MKLQTCRLSLWFLLFLISFCFCSFSVALKKNQQLKKKTYIVHMDRTNMPLAFDDHFQWYD 63
           MK+QTCR+S WFLLFLIS C CSF+ A K +QQLKKKTYI+HMDRTNMP AFDDHFQWYD
Sbjct: 1   MKIQTCRVSQWFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYD 60

Query: 64  SSLKSVSDSAQMLYSYNTVVHGFSTRLTVEEAKLIEKQLGILAVVPEEKYELHTTRTPEF 123
           SSLKSVSDSAQ LY YNTVVHGFSTRLTVEEAKLIEKQ GILAV+PE KY+LHTTRTPEF
Sbjct: 61  SSLKSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEF 120

Query: 124 LGLGKSVSFLPASEKVGEVIIGVLDTGVWPELESFNDAGLGPVPASWKGECEVGKNFTSS 183
           LGL KSVSF PAS KVGEVI+GVLDTGV PELESF+D GLGPVP SWKGECEVGKNF+SS
Sbjct: 121 LGLEKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSS 180

Query: 184 SCNRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGTHTSTTAAGSAVTGANLFGYA 243
           SCNRKLIGARYFSKGYEAAFG IDESQESKSPRDDDGHG+HTSTTAAGSAVTGA+LFG+A
Sbjct: 181 SCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFA 240

Query: 244 AGTARGMAAEARVATYKVCWLGGCFGSDILAAMDKAVGDGVNVLSLSLGGSSPDYYRDNV 303
           AGTA+GMAAEARVATYKVCWLGGCFGSDILAAMDKA+ DGVNVLSLSLGGSSPDYYRDNV
Sbjct: 241 AGTAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNV 300

Query: 304 AIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKFT 363
           AIGAFSA AQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFP YVTLGNGKKFT
Sbjct: 301 AIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFT 360

Query: 364 GESLYSGKPLPDSLIPIVYAGNASNSTSGSLCLTSTLIPAKVAGKIVVCDRGGNSRVQKG 423
           G+SLY+GKPL DSLIPIVYA +ASNSTSGSLCLTSTL PAKVAGKIVVCDRGGNSRVQKG
Sbjct: 361 GQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKG 420

Query: 424 VVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKAGDAIKSYISSDANPTATISTG 483
           +VVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQK GDAIKSYIS++ANPTATIS G
Sbjct: 421 LVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISAG 480

Query: 484 TTRLGVQPSPVVAAFSSRGPNFLTPQILKPDLIAPGVNILAGWTGGAGPTGSEDDKRRVP 543
           TTRLGVQPSP+VAAFSSRGPN LTPQILKPDLIAPGVNILAGW GG GPTG + DKR V 
Sbjct: 481 TTRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWAGGVGPTGLDSDKRHVA 540

Query: 544 FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTSYSTYKSGEAIQDISNGLAS 603
           FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTT+YSTYK+GEAIQD+S+GL S
Sbjct: 541 FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLPS 600

Query: 604 TPFDIGAGHVNPTAALDPGLVYDTTTDDYFAFLCALNYSSLQIKVIAKKDFTCSANKNYR 663
           TPFDIGAGHVNPTAALDPGLVYD T +DYFAFLCALNYSS QIKVI+KKDFTCS NKNY+
Sbjct: 601 TPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSRNKNYK 660

Query: 664 LEDLNYPSFAVPLETPSTRGGGD-AAPTTVKYTRTLTNKGASSTYKATVTSKSPSVKIVV 723
           LEDLNYPSFAV LETPST+GGG+  APTTVKYTRTLTNKGA STYK +VTSKSPSVKI+V
Sbjct: 661 LEDLNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIMV 720

Query: 724 EPESLSFAEANEQKSYTVTFIAFPMPSGTVSFARLEWSDGKHVVGSPIAFTWT 776
           EPESLSFA+ NEQKSYTVTF+A  MPSG+ SFARLEWSDGKH VGSPIAFTWT
Sbjct: 721 EPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT 773

BLAST of Spg025357 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 996.9 bits (2576), Expect = 1.3e-289
Identity = 498/764 (65.18%), Postives = 600/764 (78.53%), Query Frame = 0

Query: 14  WFLLFLISFCFCSFSVALKKNQQLKKKTYIVHMDRTNMPLAFDDHFQWYDSSLKSVSDSA 73
           +FLL  + FC  S S +        + TYIVHM ++ MP +FD H  WYDSSL+S+SDSA
Sbjct: 11  FFLLLCLGFCHVSSSSS-------DQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSA 70

Query: 74  QMLYSYNTVVHGFSTRLTVEEAKLIEKQLGILAVVPEEKYELHTTRTPEFLGLGK-SVSF 133
           ++LY+Y   +HGFSTRLT EEA  +  Q G+++V+PE +YELHTTRTP FLGL + +   
Sbjct: 71  ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL 130

Query: 134 LPASEKVGEVIIGVLDTGVWPELESFNDAGLGPVPASWKGECEVGKNFTSSSCNRKLIGA 193
            P +    +V++GVLDTGVWPE +S++D G GP+P+SWKG CE G NFT+S CNRKLIGA
Sbjct: 131 FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGA 190

Query: 194 RYFSKGYEAAFGPIDESQESKSPRDDDGHGTHTSTTAAGSAVTGANLFGYAAGTARGMAA 253
           R+F++GYE+  GPIDES+ES+SPRDDDGHGTHTS+TAAGS V GA+L GYA+GTARGMA 
Sbjct: 191 RFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAP 250

Query: 254 EARVATYKVCWLGGCFGSDILAAMDKAVGDGVNVLSLSLGGSSPDYYRDNVAIGAFSAAA 313
            ARVA YKVCWLGGCF SDILAA+DKA+ D VNVLS+SLGG   DYYRD VAIGAF+A  
Sbjct: 251 RARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAME 310

Query: 314 QGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKFTGESLYSGKP 373
           +G+ VSCSAGN GPSS SLSNVAPWITTVGAGTLDRDFPA   LGNGK FTG SL+ G+ 
Sbjct: 311 RGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEA 370

Query: 374 LPDSLIPIVYAGNASNSTSGSLCLTSTLIPAKVAGKIVVCDRGGNSRVQKGVVVKEAGGA 433
           LPD L+P +YAGNASN+T+G+LC+T TLIP KV GKIV+CDRG N+RVQKG VVK AGG 
Sbjct: 371 LPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGV 430

Query: 434 GMILANTETYGEEQLADAHLLPAAAVGQKAGDAIKSYISSDANPTATISTGTTRLGVQPS 493
           GMILANT   GEE +ADAHLLPA  VG+KAGD I+ Y+++D NPTA+IS   T +GV+PS
Sbjct: 431 GMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPS 490

Query: 494 PVVAAFSSRGPNFLTPQILKPDLIAPGVNILAGWTGGAGPTGSEDDKRRVPFNIISGTSM 553
           PVVAAFSSRGPN +TP ILKPDLIAPGVNILA WTG AGPTG   D RRV FNIISGTSM
Sbjct: 491 PVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSM 550

Query: 554 SCPHISGLAALLKAAHPDWSPAAIRSALMTTSYSTYKSGEAIQDISNGLASTPFDIGAGH 613
           SCPH+SGLAALLK+ HP+WSPAAIRSALMTT+Y TYK G+ + DI+ G  STPFD GAGH
Sbjct: 551 SCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGH 610

Query: 614 VNPTAALDPGLVYDTTTDDYFAFLCALNYSSLQIKVIAKKDFTCSANKNYRLEDLNYPSF 673
           V+PT A +PGL+YD TT+DY  FLCALNY+S QI+ ++++++TC  +K+Y + DLNYPSF
Sbjct: 611 VSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSF 670

Query: 674 AVPLETPSTRGGGDAAPTTVKYTRTLTNKGASSTYKATVTSKSPSVKIVVEPESLSFAEA 733
           AV ++     G G       KYTRT+T+ G + TY   VTS++  VKI VEP  L+F EA
Sbjct: 671 AVNVD-----GVG-----AYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEA 730

Query: 734 NEQKSYTVTF-IAFPMPSGTVSFARLEWSDGKHVVGSPIAFTWT 776
           NE+KSYTVTF +    PSG+ SF  +EWSDGKHVVGSP+A +WT
Sbjct: 731 NEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISWT 757

BLAST of Spg025357 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 770.4 bits (1988), Expect = 1.9e-221
Identity = 417/741 (56.28%), Postives = 509/741 (68.69%), Query Frame = 0

Query: 39  KKTYIVHMDRTNMPLAFDDHFQWYDSSLKSVSDSAQMLYSYNTVVHGFSTRLTVEEA-KL 98
           KKTYI+ ++ ++ P +F  H  WY S L S S    +LY+Y T  HGFS  L   EA  L
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES---SLLYTYTTSFHGFSAYLDSTEADSL 86

Query: 99  IEKQLGILAVVPEEKYELHTTRTPEFLGLGKSVSFLPASEKVGEVIIGVLDTGVWPELES 158
           +     IL +  +  Y LHTTRTPEFLGL               VIIGVLDTGVWPE  S
Sbjct: 87  LSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPESRS 146

Query: 159 FNDAGLGPVPASWKGECEVGKNFTSSSCNRKLIGARYFSKGYE-AAFGPIDESQESKSPR 218
           F+D  +  +P+ WKGECE G +F S  CN+KLIGAR FSKG++ A+ G     +ES SPR
Sbjct: 147 FDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESVSPR 206

Query: 219 DDDGHGTHTSTTAAGSAVTGANLFGYAAGTARGMAAEARVATYKVCWLGGCFGSDILAAM 278
           D DGHGTHTSTTAAGSAV  A+  GYAAGTARGMA  ARVATYKVCW  GCFGSDILAAM
Sbjct: 207 DVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDILAAM 266

Query: 279 DKAVGDGVNVLSLSLGGSSPDYYRDNVAIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAP 338
           D+A+ DGV+VLSLSLGG S  YYRD +AIGAFSA  +GVFVSCSAGN GP+  S++NVAP
Sbjct: 267 DRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAP 326

Query: 339 WITTVGAGTLDRDFPAYVTLGNGKKFTGESLYSGKPLPDSLIPIVYAGNASNSTSGSLCL 398
           W+ TVGAGTLDRDFPA+  LGNGK+ TG SLYSG  +    + +VY  N  NS+S +LCL
Sbjct: 327 WVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVY--NKGNSSSSNLCL 386

Query: 399 TSTLIPAKVAGKIVVCDRGGNSRVQKGVVVKEAGGAGMILANTETYGEEQLADAHLLPAA 458
             +L  + V GKIVVCDRG N+RV+KG VV++AGG GMI+ANT   GEE +AD+HLLPA 
Sbjct: 387 PGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAI 446

Query: 459 AVGQKAGDAIKSYISSDANPTATISTGTTRLGVQPSPVVAAFSSRGPNFLTPQILKPDLI 518
           AVG+K GD ++ Y+ SD+ PTA +    T L V+PSPVVAAFSSRGPN +TP+ILKPD+I
Sbjct: 447 AVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVI 506

Query: 519 APGVNILAGWTGGAGPTGSEDDKRRVPFNIISGTSMSCPHISGLAALLKAAHPDWSPAAI 578
            PGVNILAGW+   GPTG + D RR  FNI+SGTSMSCPHISGLA LLKAAHP+WSP+AI
Sbjct: 507 GPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAI 566

Query: 579 RSALMTTSYSTYKSGEAIQDISNGLASTPFDIGAGHVNPTAALDPGLVYDTTTDDYFAFL 638
           +SALMTT+Y    +   + D ++   S P+  G+GHV+P  AL PGLVYD +T++Y  FL
Sbjct: 567 KSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFL 626

Query: 639 CALNYSSLQIKVIAKKDFTCSANKNYRLEDLNYPSFAVPLETPSTRGGGDAAPTTVKYTR 698
           C+L+Y+   I  I K+     + K      LNYPSF+V         GG      V+YTR
Sbjct: 627 CSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSVLF-------GG---KRVVRYTR 686

Query: 699 TLTNKG-ASSTYKATVTSKSPSVKIVVEPESLSFAEANEQKSYTVTFIAFPMPSGT--VS 758
            +TN G ASS YK TV   +PSV I V+P  LSF    E+K YTVTF++    S T    
Sbjct: 687 EVTNVGAASSVYKVTVNG-APSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAE 746

Query: 759 FARLEWSDGKHVVGSPIAFTW 775
           F  + WS+ +H V SP+AF+W
Sbjct: 747 FGSITWSNPQHEVRSPVAFSW 751

BLAST of Spg025357 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 751.1 bits (1938), Expect = 1.2e-215
Identity = 405/775 (52.26%), Postives = 515/775 (66.45%), Query Frame = 0

Query: 17  LFLISFCFCSFSVALKKNQQLKKKTYIVHMDRTNMPLAFDDHFQWYDSSLKSVS------ 76
           LF+I      F  A    Q   KKTY++HMD++ MPL + +H QWY S + SV+      
Sbjct: 12  LFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQE 71

Query: 77  ---DSAQMLYSYNTVVHGFSTRLTVEEAKLIEKQLGILAVVPEEKYELHTTRTPEFLGLG 136
              ++ ++LY+Y T  HG + +LT EEA+ +E++ G++AV+PE +YELHTTR+P FLGL 
Sbjct: 72  EEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLE 131

Query: 137 KSVSFLPASEKV--GEVIIGVLDTGVWPELESFNDAGLGPVPASWKGECEVGKNFTSSSC 196
           +  S    +E+V   +V++GVLDTG+WPE ESFND G+ PVPA+W+G CE GK F   +C
Sbjct: 132 RQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNC 191

Query: 197 NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGTHTSTTAAGSAVTGANLFGYAAG 256
           NRK++GAR F +GYEAA G IDE  E KSPRD DGHGTHT+ T AGS V GANLFG+A G
Sbjct: 192 NRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYG 251

Query: 257 TARGMAAEARVATYKVCWLGGCFGSDILAAMDKAVGDGVNVLSLSLGGSSPDYYRDNVAI 316
           TARGMA +ARVA YKVCW+GGCF SDIL+A+D+AV DGV VLS+SLGG    Y RD+++I
Sbjct: 252 TARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSI 311

Query: 317 GAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKFTGE 376
             F A   GVFVSCSAGNGGP   SL+NV+PWITTVGA T+DRDFPA V +G  + F G 
Sbjct: 312 ATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGV 371

Query: 377 SLYSGKPL--PDSLIPIVYAG-NASNSTSGSLCLTSTLIPAKVAGKIVVCDRGGNSRVQK 436
           SLY G+ +   +   P+VY G NAS+    S CL   L    VAGKIV+CDRG   RVQK
Sbjct: 372 SLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQK 431

Query: 437 GVVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKAGDAIKSYISSDANPTATIST 496
           G VVK AGG GM+L NT T GEE +AD+H+LPA AVG+K G  IK Y  +    TA++  
Sbjct: 432 GQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEI 491

Query: 497 GTTRLGVQPSPVVAAFSSRGPNFLTPQILKPDLIAPGVNILAGWTGGAGPTGSEDDKRRV 556
             TR+G++PSPVVAAFSSRGPNFL+ +ILKPDL+APGVNILA WTG   P+    D RRV
Sbjct: 492 LGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRV 551

Query: 557 PFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTSYSTYKSGEAIQDISNGLA 616
            FNI+SGTSMSCPH+SG+AAL+K+ HPDWSPAAI+SALMTT+Y      + + D S    
Sbjct: 552 KFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAP 611

Query: 617 STPFDIGAGHVNPTAALDPGLVYDTTTDDYFAFLCALNYSSLQIKVIAK-KDFTCSANKN 676
           S+P+D GAGH++P  A DPGLVYD    +YF FLC  + S  Q+KV  K  + TC     
Sbjct: 612 SSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLA 671

Query: 677 YRLEDLNYPSFAVPLETPSTRGGGDAAPTTVKYTRTLTNKGAS-STYKATVTSKSPSVKI 736
               +LNYP+ +            +     +   RT+TN G   S+YK +V S      +
Sbjct: 672 KNPGNLNYPAISALFPE-------NTHVKAMTLRRTVTNVGPHISSYKVSV-SPFKGASV 731

Query: 737 VVEPESLSFAEANEQKSYTVTF-IAFPMPSGTVSFARLEWSDGKHVVGSPIAFTW 775
            V+P++L+F   +++ SYTVTF   F M      F  L W    H V SP+  TW
Sbjct: 732 TVQPKTLNFTSKHQKLSYTVTFRTRFRMK--RPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of Spg025357 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 739.6 bits (1908), Expect = 3.7e-212
Identity = 402/782 (51.41%), Postives = 523/782 (66.88%), Query Frame = 0

Query: 10  RLSLWFLLFLISFCFCSFSVALKKNQQLKKKTYIVHMDRTNMPLAFDDHFQWYDSSLKSV 69
           +LSL  + F+     C FS +   +  L  ++YIVH+ R++ P  F  H  W+ S L+S+
Sbjct: 3   KLSLSSIFFVFPLLLCFFSPSSSSSDGL--ESYIVHVQRSHKPSLFSSHNNWHVSLLRSL 62

Query: 70  SDSAQ---MLYSYNTVVHGFSTRLTVEEAKLIEKQLGILAVVPEEKYELHTTRTPEFLGL 129
             S Q   +LYSY+  VHGFS RL+  +   + +   +++V+P++  E+HTT TP FLG 
Sbjct: 63  PSSPQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGF 122

Query: 130 GKSVSFLPASEKVGEVIIGVLDTGVWPELESFNDAGLGPVPASWKGECEVGKNFTSSSCN 189
            ++      S    +VI+GVLDTG+WPE  SF+D+GLGP+P++WKGECE+G +F +SSCN
Sbjct: 123 SQNSGLWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCN 182

Query: 190 RKLIGARYFSKGYEAAFGPIDE--SQESKSPRDDDGHGTHTSTTAAGSAVTGANLFGYAA 249
           RKLIGAR F +GY        +  ++ES+SPRD +GHGTHT++TAAGS V  A+L+ YA 
Sbjct: 183 RKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYAR 242

Query: 250 GTARGMAAEARVATYKVCWLGGCFGSDILAAMDKAVGDGVNVLSLSLG--GSSPDYYRDN 309
           GTA GMA++AR+A YK+CW GGC+ SDILAAMD+AV DGV+V+SLS+G  GS+P+Y+ D+
Sbjct: 243 GTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDS 302

Query: 310 VAIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKF 369
           +AIGAF A   G+ VSCSAGN GP+  + +N+APWI TVGA T+DR+F A    G+GK F
Sbjct: 303 IAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVF 362

Query: 370 TGESLYSGKPLPDSLIPIVYAGNASNSTSGSLCLTSTLIPAKVAGKIVVCDRGGNSRVQK 429
           TG SLY+G+ LPDS + +VY+G+  +     LC    L  + V GKIV+CDRGGN+RV+K
Sbjct: 363 TGTSLYAGESLPDSQLSLVYSGDCGS----RLCYPGKLNSSLVEGKIVLCDRGGNARVEK 422

Query: 430 GVVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKAGDAIKSYISSDANPTATIST 489
           G  VK AGGAGMILANT   GEE  AD+HL+PA  VG KAGD I+ YI +  +PTA IS 
Sbjct: 423 GSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISF 482

Query: 490 GTTRLGVQ-PSPVVAAFSSRGPNFLTPQILKPDLIAPGVNILAGWTGGAGPTGSEDDKRR 549
             T +G   PSP VAAFSSRGPN LTP ILKPD+IAPGVNILAGWTG  GPT  + D RR
Sbjct: 483 LGTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRR 542

Query: 550 VPFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTSYSTYKSGEAIQDISNGL 609
           V FNIISGTSMSCPH+SGLAALL+ AHPDWSPAAI+SAL+TT+Y    SGE I+D++ G 
Sbjct: 543 VQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGK 602

Query: 610 ASTPFDIGAGHVNPTAALDPGLVYDTTTDDYFAFLCALNYSSLQIKVIAKKDF---TCSA 669
           +S  F  GAGHV+P  AL+PGLVYD    +Y AFLCA+ Y    I V  +       C  
Sbjct: 603 SSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACET 662

Query: 670 NKNYRLEDLNYPSFAVPLETPSTRGGGDAAPTTVKYTRTLTNKGASSTYKATVTSKSP-S 729
           +K     DLNYPSF+V   +             VKY R + N G++      V  KSP +
Sbjct: 663 SKLRTAGDLNYPSFSVVFASTG---------EVVKYKRVVKNVGSNVDAVYEVGVKSPAN 722

Query: 730 VKIVVEPESLSFAEANEQKSYTVTFIAFPMPSGTVS-----FARLEWSDGKHVVGSPIAF 775
           V+I V P  L+F++      Y VTF +  +  G  S     F  +EW+DG+HVV SP+A 
Sbjct: 723 VEIDVSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAV 769

BLAST of Spg025357 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 707.2 bits (1824), Expect = 2.0e-202
Identity = 389/777 (50.06%), Postives = 505/777 (64.99%), Query Frame = 0

Query: 14  WFLLFLISFCFCSFSVALKKNQQLKKKTYIVHMDRTNMPLAFDDHFQWYDSSLKSVSDS- 73
           +F  F       S S +   +  L   TYIVH+D    P  F  HF WY SSL S++ S 
Sbjct: 3   FFFYFFFLLTLSSPSSSASSSNSL---TYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSP 62

Query: 74  AQMLYSYNTVVHGFSTRLTVEEAKLIEKQLGILAVVPEEKYELHTTRTPEFLGLGKS--V 133
             ++++Y+TV HGFS RLT ++A  +     +++V+PE+   LHTTR+PEFLGL  +   
Sbjct: 63  PSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKA 122

Query: 134 SFLPASEKVGEVIIGVLDTGVWPELESFNDAGLGPVPASWKGECEVGKNFTSSSCNRKLI 193
             L  S+   +++IGV+DTGVWPE  SF+D GLGPVP  WKG+C   ++F  S+CNRKL+
Sbjct: 123 GLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLV 182

Query: 194 GARYFSKGYEAAFGPIDESQESKSPRDDDGHGTHTSTTAAGSAVTGANLFGYAAGTARGM 253
           GAR+F  GYEA  G ++E+ E +SPRD DGHGTHT++ +AG  V  A+  GYA G A GM
Sbjct: 183 GARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGM 242

Query: 254 AAEARVATYKVCWLGGCFGSDILAAMDKAVGDGVNVLSLSLGGSSPDYYRDNVAIGAFSA 313
           A +AR+A YKVCW  GC+ SDILAA D AV DGV+V+SLS+GG    YY D +AIGAF A
Sbjct: 243 APKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGA 302

Query: 314 AAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKFTGESLYSG 373
             +G+FVS SAGNGGP + +++NVAPW+TTVGAGT+DRDFPA V LGNGK  +G S+Y G
Sbjct: 303 IDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGG 362

Query: 374 KPL-PDSLIPIVYAGN--ASNSTSGSLCLTSTLIPAKVAGKIVVCDRGGNSRVQKGVVVK 433
             L P  + P+VY G+    +  S SLCL  +L P  V GKIV+CDRG NSR  KG +V+
Sbjct: 363 PGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVR 422

Query: 434 EAGGAGMILANTETYGEEQLADAHLLPAAAVGQKAGDAIKSYIS------SDANPTATIS 493
           + GG GMI+AN    GE  +AD H+LPA +VG   GD I+ YIS      S  +PTATI 
Sbjct: 423 KNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIV 482

Query: 494 TGTTRLGVQPSPVVAAFSSRGPNFLTPQILKPDLIAPGVNILAGWTGGAGPTGSEDDKRR 553
              TRLG++P+PVVA+FS+RGPN  TP+ILKPD+IAPG+NILA W    GP+G   D RR
Sbjct: 483 FKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRR 542

Query: 554 VPFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTSYSTYKSGEAIQDISNGL 613
             FNI+SGTSM+CPH+SGLAALLKAAHPDWSPAAIRSAL+TT+Y+   SGE + D S G 
Sbjct: 543 TEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGN 602

Query: 614 ASTPFDIGAGHVNPTAALDPGLVYDTTTDDYFAFLCALNYSSLQIKVIAKKDFTC-SANK 673
            S+  D G+GHV+PT A+DPGLVYD T+ DY  FLC  NY+   I  I ++   C  A +
Sbjct: 603 TSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARR 662

Query: 674 NYRLEDLNYPSFAVPLETPSTRGGGDAAPTTVKYTRTLTNKGASSTYKATVTSKSPSVKI 733
              + +LNYPSF+V  +       G++  +T  + RT+TN G S +             +
Sbjct: 663 AGHVGNLNYPSFSVVFQQ-----YGESKMST-HFIRTVTNVGDSDSVYEIKIRPPRGTTV 722

Query: 734 VVEPESLSFAEANEQKSYTV----TFIAFPMPSGTVSFARLEWSDGKHVVGSPIAFT 774
            VEPE LSF    ++ S+ V    T +     +  V    + WSDGK  V SP+  T
Sbjct: 723 TVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770

BLAST of Spg025357 vs. ExPASy TrEMBL
Match: A0A6J1BPX8 (subtilisin-like protease SBT1.7 OS=Momordica charantia OX=3673 GN=LOC111004737 PE=3 SV=1)

HSP 1 Score: 1389.4 bits (3595), Expect = 0.0e+00
Identity = 701/775 (90.45%), Postives = 734/775 (94.71%), Query Frame = 0

Query: 1   MKKMKLQTCRLSLWFLLFLISFCFCSFSVALKKNQQLKKKTYIVHMDRTNMPLAFDDHFQ 60
           MKKMK+QTCR+S  FLLFLI F   S+  A K NQ+LKKKTYI+HMDRTNMP AFDDHFQ
Sbjct: 1   MKKMKMQTCRVSRLFLLFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQ 60

Query: 61  WYDSSLKSVSDSAQMLYSYNTVVHGFSTRLTVEEAKLIEKQLGILAVVPEEKYELHTTRT 120
           WYDSSLKSVSDSAQMLYSY+TV+HGFSTRLTVEEA+L+EK+ GILAV+PE KYELHTTRT
Sbjct: 61  WYDSSLKSVSDSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRT 120

Query: 121 PEFLGLGKSVSFLPASEKVGEVIIGVLDTGVWPELESFNDAGLGPVPASWKGECEVGKNF 180
           PEFLGLGKS SF PAS KVGEVIIG+LDTGVWPELESFNDAGLGPVP SWKGECEVGKNF
Sbjct: 121 PEFLGLGKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNF 180

Query: 181 TSSSCNRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGTHTSTTAAGSAVTGANLF 240
           +SSSCNRKLIGARYF+KGYE+AFGPIDESQESKSP+DDDGHGTHTSTTAAGSAVTGANLF
Sbjct: 181 SSSSCNRKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLF 240

Query: 241 GYAAGTARGMAAEARVATYKVCWLGGCFGSDILAAMDKAVGDGVNVLSLSLGGSSPDYYR 300
           GYA+GTARGMAA+ARVATYKVCWLGGCFGSDILAA+DKAV DGVNVLSLSLGGSSPDYYR
Sbjct: 241 GYASGTARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYR 300

Query: 301 DNVAIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYVTLGNGK 360
           DNVAIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAY+TLGNGK
Sbjct: 301 DNVAIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGK 360

Query: 361 KFTGESLYSGKPLPDSLIPIVYAGNASNSTSGSLCLTSTLIPAKVAGKIVVCDRGGNSRV 420
           KFTGESLYSGKPLPDSL+PIVYA +ASNSTSGS CLT TL PAKVAGKIVVCDRGGNSRV
Sbjct: 361 KFTGESLYSGKPLPDSLVPIVYAAHASNSTSGSSCLTGTLNPAKVAGKIVVCDRGGNSRV 420

Query: 421 QKGVVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKAGDAIKSYISSDANPTATI 480
           QKGVVVKEAGGAGMILANTETYGEE+LADAHLLPAAAVGQK GDAIKSYISSDANPTA  
Sbjct: 421 QKGVVVKEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKF 480

Query: 481 STGTTRLGVQPSPVVAAFSSRGPNFLTPQILKPDLIAPGVNILAGWTGGAGPTGSEDDKR 540
           STGTTRLGVQPSPVVAAFSSRGPN LTP ILKPDLIAPGVNILAGWTG AGPTG + DKR
Sbjct: 481 STGTTRLGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKR 540

Query: 541 RVPFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTSYSTYKSGEAIQDISNG 600
           RV FNIISGTSMSCPHISGLAAL+KAAHPDWSPAAIRSALMTT+YSTYK+GEAIQDIS+G
Sbjct: 541 RVAFNIISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSG 600

Query: 601 LASTPFDIGAGHVNPTAALDPGLVYDTTTDDYFAFLCALNYSSLQIKVIAKKDFTCSANK 660
           L STPFDIGAGHVNP AALDPGLVYDTTTDDYF FLCALNY+SLQIKVIAKKDFTCSA+K
Sbjct: 601 LPSTPFDIGAGHVNPGAALDPGLVYDTTTDDYFVFLCALNYTSLQIKVIAKKDFTCSASK 660

Query: 661 NYRLEDLNYPSFAVPLETPSTRGGGDAAPTTVKYTRTLTNKGASSTYKATVTSKSPSVKI 720
           NY+LEDLNYPSFAVPLETPS RGGGDAAPTTVKYTRTLTNK ASSTYK + TSKSPSVKI
Sbjct: 661 NYKLEDLNYPSFAVPLETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVSETSKSPSVKI 720

Query: 721 VVEPESLSFAEANEQKSYTVTFIAFPMPSGTVSFARLEWSDGKHVVGSPIAFTWT 776
           VVEPESLSFAE NEQKSYTVTFIA PMPSGT SFARLEWSDGKH+VGSPIAFTWT
Sbjct: 721 VVEPESLSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFTWT 775

BLAST of Spg025357 vs. ExPASy TrEMBL
Match: A0A0A0LKN0 (Xylem serine proteinase 1 OS=Cucumis sativus OX=3659 GN=Csa_2G354860 PE=3 SV=1)

HSP 1 Score: 1375.5 bits (3559), Expect = 0.0e+00
Identity = 686/770 (89.09%), Postives = 727/770 (94.42%), Query Frame = 0

Query: 6   LQTCRLSLWFLLFLISFCFCSFSVALKKNQQLKKKTYIVHMDRTNMPLAFDDHFQWYDSS 65
           ++TCR+S WFLLFLISFC CSF+ A K NQQLKKKTYI+HMD+TNMP AFDDHFQWYDSS
Sbjct: 1   MKTCRVSQWFLLFLISFCSCSFTEAQKSNQQLKKKTYIIHMDKTNMPQAFDDHFQWYDSS 60

Query: 66  LKSVSDSAQMLYSYNTVVHGFSTRLTVEEAKLIEKQLGILAVVPEEKYELHTTRTPEFLG 125
           LKSVSDSAQMLYSYNTV+HGFSTRLTVEEAKL+EKQ GI+AV+PE KYELHTTRTPEFLG
Sbjct: 61  LKSVSDSAQMLYSYNTVIHGFSTRLTVEEAKLMEKQEGIIAVIPEMKYELHTTRTPEFLG 120

Query: 126 LGKSVSFLPASEKVGEVIIGVLDTGVWPELESFNDAGLGPVPASWKGECEVGKNFTSSSC 185
           LGKSVSF PASEKV EVIIGVLDTGVWPELESF+DAGLGP+PASWKGECEVGKNFTSS+C
Sbjct: 121 LGKSVSFFPASEKVSEVIIGVLDTGVWPELESFSDAGLGPIPASWKGECEVGKNFTSSNC 180

Query: 186 NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGTHTSTTAAGSAVTGANLFGYAAG 245
           NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHG+HTSTTAAGSAVTGANLFG+AAG
Sbjct: 181 NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAG 240

Query: 246 TARGMAAEARVATYKVCWLGGCFGSDILAAMDKAVGDGVNVLSLSLGGSSPDYYRDNVAI 305
           TARGMAAEARVATYKVCWLGGCF SDILAAMDK+V DG N+LS+SLGG+S DYYRDNVAI
Sbjct: 241 TARGMAAEARVATYKVCWLGGCFSSDILAAMDKSVEDGCNILSVSLGGNSADYYRDNVAI 300

Query: 306 GAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKFTGE 365
           GAFSA AQGVFVSCSAGNGGPSS +LSNVAPWITTVGAGTLDRDFPAYVTLGNGKK TGE
Sbjct: 301 GAFSATAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGE 360

Query: 366 SLYSGKPLPDSLIPIVYAGNASNSTSGSLCLTSTLIPAKVAGKIVVCDRGGNSRVQKGVV 425
           SLYSGKPLP+SL+PIV A +ASNS+SGSLCL+ TL PAKV GKIVVCDRGGNSRVQKGVV
Sbjct: 361 SLYSGKPLPNSLLPIVSAASASNSSSGSLCLSGTLNPAKVTGKIVVCDRGGNSRVQKGVV 420

Query: 426 VKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKAGDAIKSYISSDANPTATISTGTT 485
           VKEAGG GMILANTE YGEEQLADAHL+P AAVGQKAGDAIK+YISSD+NPTATISTGTT
Sbjct: 421 VKEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQKAGDAIKNYISSDSNPTATISTGTT 480

Query: 486 RLGVQPSPVVAAFSSRGPNFLTPQILKPDLIAPGVNILAGWTGGAGPTGSEDDKRRVPFN 545
           RLGVQPSPVVAAFSSRGPN LTPQILKPDLIAPGVNILAGWTGGAGPTG + DKR V FN
Sbjct: 481 RLGVQPSPVVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFN 540

Query: 546 IISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTSYSTYKSGEAIQDISNGLASTP 605
           IISGTSMSCPHISGLAAL+KAAHPDWSPAAIRSALMTT+YSTYK+GE IQDISNG  STP
Sbjct: 541 IISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEMIQDISNGSPSTP 600

Query: 606 FDIGAGHVNPTAALDPGLVYDTTTDDYFAFLCALNYSSLQIKVIAKKDFTCSANKNYRLE 665
           FDIGAGHVNPTAALDPGLVYDTTTDDY AFLCALNYSSLQIKVI+KKDFTC+ NKNY+LE
Sbjct: 601 FDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLE 660

Query: 666 DLNYPSFAVPLETPSTRGGGDAAPTTVKYTRTLTNKGASSTYKATVTSKSPSVKIVVEPE 725
           DLNYPSFAVPLETPSTRGG + APTT+KYTRTLTNKGASSTYK +VT+KS SVKIVVEPE
Sbjct: 661 DLNYPSFAVPLETPSTRGGENVAPTTIKYTRTLTNKGASSTYKVSVTAKSSSVKIVVEPE 720

Query: 726 SLSFAEANEQKSYTVTFIAFPMPSGTVSFARLEWSDGKHVVGSPIAFTWT 776
           SLSF E NEQKSYTVTFIA PMPSG+ SFARLEWSDGKH+VGSPIAFTWT
Sbjct: 721 SLSFTEVNEQKSYTVTFIASPMPSGSQSFARLEWSDGKHIVGSPIAFTWT 770

BLAST of Spg025357 vs. ExPASy TrEMBL
Match: A0A6J1HBB4 (subtilisin-like protease SBT1.7 OS=Cucurbita moschata OX=3662 GN=LOC111462526 PE=3 SV=1)

HSP 1 Score: 1355.9 bits (3508), Expect = 0.0e+00
Identity = 684/773 (88.49%), Postives = 723/773 (93.53%), Query Frame = 0

Query: 4   MKLQTCRLSLWFLLFLISFCFCSFSVALKKNQQLKKKTYIVHMDRTNMPLAFDDHFQWYD 63
           MK+QTCR+S WFLLFLIS C CSF+ A K +QQLKKKTYI+HMDRTNMP AFDDHFQWYD
Sbjct: 1   MKIQTCRVSQWFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYD 60

Query: 64  SSLKSVSDSAQMLYSYNTVVHGFSTRLTVEEAKLIEKQLGILAVVPEEKYELHTTRTPEF 123
           SSLKSVSDSAQ LY YNTVVHGFSTRLTVEEAKLIEKQ GILAV+PE KY+LHTTRTPEF
Sbjct: 61  SSLKSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEF 120

Query: 124 LGLGKSVSFLPASEKVGEVIIGVLDTGVWPELESFNDAGLGPVPASWKGECEVGKNFTSS 183
           LGL KSVSF PAS KVGEVI+GVLDTGV PELESF+D GLGPVP SWKGECEVGKNF+SS
Sbjct: 121 LGLEKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSS 180

Query: 184 SCNRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGTHTSTTAAGSAVTGANLFGYA 243
           SCNRKLIGARYFSKGYEAAFG IDESQESKSPRDDDGHG+HTSTTAAGSAVTGA+LFG+ 
Sbjct: 181 SCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFG 240

Query: 244 AGTARGMAAEARVATYKVCWLGGCFGSDILAAMDKAVGDGVNVLSLSLGGSSPDYYRDNV 303
           AGTA+GMAAEARVATYKVCWLGGCFGSDILAAMDKA+ DGVNVLSLSLGGSSPDYYRDNV
Sbjct: 241 AGTAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNV 300

Query: 304 AIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKFT 363
           AIGAFSA AQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFP YVTLGNGKKFT
Sbjct: 301 AIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFT 360

Query: 364 GESLYSGKPLPDSLIPIVYAGNASNSTSGSLCLTSTLIPAKVAGKIVVCDRGGNSRVQKG 423
           G+SLY+GKPL DSLIPIVYA +ASNSTSGSLCLTSTL PAKVAGKIVVCDRGGNSRVQKG
Sbjct: 361 GQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKG 420

Query: 424 VVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKAGDAIKSYISSDANPTATISTG 483
           +VVK+AGGAGMILANTETYGEEQLADAHLLPAAAVGQK GDAIKSYIS++ANPTATIS G
Sbjct: 421 LVVKDAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISAG 480

Query: 484 TTRLGVQPSPVVAAFSSRGPNFLTPQILKPDLIAPGVNILAGWTGGAGPTGSEDDKRRVP 543
           +TRLGVQPSP+VAAFSSRGPN LTPQILKPDLIAPGVNILAGWT G GPTG + DKR V 
Sbjct: 481 STRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWTSGVGPTGLDSDKRHVA 540

Query: 544 FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTSYSTYKSGEAIQDISNGLAS 603
           FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTT+YSTYK+GEAIQD+S+GL S
Sbjct: 541 FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLPS 600

Query: 604 TPFDIGAGHVNPTAALDPGLVYDTTTDDYFAFLCALNYSSLQIKVIAKKDFTCSANKNYR 663
           TPFDIGAGHVNPTAALDPGLVYD T +DYFAFLCALNYSS QIKVI+KKDFTCS NKNY+
Sbjct: 601 TPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKNYK 660

Query: 664 LEDLNYPSFAVPLETPSTRGGGD-AAPTTVKYTRTLTNKGASSTYKATVTSKSPSVKIVV 723
           LEDLNYPSFAV LETPST+GG +  APTTVKYTRTLTNKGA STYK +VTSKSPSVKI+V
Sbjct: 661 LEDLNYPSFAVALETPSTKGGAEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIMV 720

Query: 724 EPESLSFAEANEQKSYTVTFIAFPMPSGTVSFARLEWSDGKHVVGSPIAFTWT 776
           EPESLSFA+ NEQKSYTVTF+A  MPSG+ SFARLEWSDGKH VGSPIAFTWT
Sbjct: 721 EPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT 773

BLAST of Spg025357 vs. ExPASy TrEMBL
Match: A0A6J1KAX6 (subtilisin-like protease SBT1.7 OS=Cucurbita maxima OX=3661 GN=LOC111491728 PE=3 SV=1)

HSP 1 Score: 1354.7 bits (3505), Expect = 0.0e+00
Identity = 686/773 (88.75%), Postives = 720/773 (93.14%), Query Frame = 0

Query: 4   MKLQTCRLSLWFLLFLISFCFCSFSVALKKNQQLKKKTYIVHMDRTNMPLAFDDHFQWYD 63
           MK+QTCR+S WFLLFLIS C CS S A  +    KKKTYI+HMDRTNMP AFDDHFQWYD
Sbjct: 1   MKIQTCRVSQWFLLFLISSCSCSCSFAAAQ----KKKTYIIHMDRTNMPQAFDDHFQWYD 60

Query: 64  SSLKSVSDSAQMLYSYNTVVHGFSTRLTVEEAKLIEKQLGILAVVPEEKYELHTTRTPEF 123
           SSLKSVSDSAQ LY YNTV HGFSTRLTVEEAKLIEKQ GILAV+PE KY+LHTTRTPEF
Sbjct: 61  SSLKSVSDSAQTLYYYNTVAHGFSTRLTVEEAKLIEKQQGILAVIPELKYDLHTTRTPEF 120

Query: 124 LGLGKSVSFLPASEKVGEVIIGVLDTGVWPELESFNDAGLGPVPASWKGECEVGKNFTSS 183
           LGL KSVSF PAS KVGEVI+GVLDTGVWPELESF+D GLGPVP SWKGECEVGKNFTSS
Sbjct: 121 LGLEKSVSFFPASVKVGEVIVGVLDTGVWPELESFDDTGLGPVPVSWKGECEVGKNFTSS 180

Query: 184 SCNRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGTHTSTTAAGSAVTGANLFGYA 243
           SCNRKLIGARYFSKGYEAAFG IDESQESKSPRDDDGHG+HTSTTAAGSAVTGA+LFG+A
Sbjct: 181 SCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFA 240

Query: 244 AGTARGMAAEARVATYKVCWLGGCFGSDILAAMDKAVGDGVNVLSLSLGGSSPDYYRDNV 303
           AGTA+GMAAEARVATYKVCWLGGCFGSDILAAMDKA+ DGVNVLSLSLGGSSPDYYRDNV
Sbjct: 241 AGTAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNV 300

Query: 304 AIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKFT 363
           AIGAFSA AQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFP YVTLGNGKKFT
Sbjct: 301 AIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFT 360

Query: 364 GESLYSGKPLPDSLIPIVYAGNASNSTSGSLCLTSTLIPAKVAGKIVVCDRGGNSRVQKG 423
           G+SLY+GKPL DSLIPIVYA +ASNSTSGSLCLTSTL PAKVAGKIVVCDRGGNSRVQKG
Sbjct: 361 GQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKG 420

Query: 424 VVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKAGDAIKSYISSDANPTATISTG 483
           +VVK+AGGAGMILANTETYGEEQLADAHLLPAAAVGQK GDAIKSYISSDANPTATIS G
Sbjct: 421 LVVKDAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISSDANPTATISAG 480

Query: 484 TTRLGVQPSPVVAAFSSRGPNFLTPQILKPDLIAPGVNILAGWTGGAGPTGSEDDKRRVP 543
           TTRLGVQPSP+VAAFSSRGPN LTPQILKPDLIAPGVNILAGWTGG GPTG + DKR V 
Sbjct: 481 TTRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWTGGVGPTGLDSDKRHVA 540

Query: 544 FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTSYSTYKSGEAIQDISNGLAS 603
           FNIISGTSMSCPHISGLAALLKAAHPDWSPA IRSALMTT+YSTYK+GEAIQD+S+GL S
Sbjct: 541 FNIISGTSMSCPHISGLAALLKAAHPDWSPAVIRSALMTTAYSTYKTGEAIQDVSSGLPS 600

Query: 604 TPFDIGAGHVNPTAALDPGLVYDTTTDDYFAFLCALNYSSLQIKVIAKKDFTCSANKNYR 663
           TPFDIGAGHVNPTAALDPGLVYD T +DYFAFLCALNYSS QIKVI+KKDFTCS NKNY+
Sbjct: 601 TPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKNYK 660

Query: 664 LEDLNYPSFAVPLETPSTRGGGD-AAPTTVKYTRTLTNKGASSTYKATVTSKSPSVKIVV 723
           LEDLNYPSFAV LETPST+GGG+  APTTVKYTRTLTNKGA STYK +VTSKSPSVKI+V
Sbjct: 661 LEDLNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIMV 720

Query: 724 EPESLSFAEANEQKSYTVTFIAFPMPSGTVSFARLEWSDGKHVVGSPIAFTWT 776
           EPESLSFA+ NEQKSYTVTF+A  MPSG+ SFARLEWSDGKH VGSPIAFTWT
Sbjct: 721 EPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT 769

BLAST of Spg025357 vs. ExPASy TrEMBL
Match: A0A1S3BA54 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103487850 PE=3 SV=1)

HSP 1 Score: 1348.2 bits (3488), Expect = 0.0e+00
Identity = 674/770 (87.53%), Postives = 719/770 (93.38%), Query Frame = 0

Query: 6   LQTCRLSLWFLLFLISFCFCSFSVALKKNQQLKKKTYIVHMDRTNMPLAFDDHFQWYDSS 65
           ++TCR+S WFLLFLISF  CSF+ A K NQQLKKKTYI+HMD+T+MP AFDDHFQWYD+S
Sbjct: 1   MKTCRVSQWFLLFLISFSSCSFTEAQKSNQQLKKKTYIIHMDKTSMPQAFDDHFQWYDTS 60

Query: 66  LKSVSDSAQMLYSYNTVVHGFSTRLTVEEAKLIEKQLGILAVVPEEKYELHTTRTPEFLG 125
           LKSVSDSAQMLYSYN V+HGFST LTVEEAKL+EKQ GI+AV+PE KYELHTTRTPEFLG
Sbjct: 61  LKSVSDSAQMLYSYNNVIHGFSTSLTVEEAKLMEKQEGIIAVMPEMKYELHTTRTPEFLG 120

Query: 126 LGKSVSFLPASEKVGEVIIGVLDTGVWPELESFNDAGLGPVPASWKGECEVGKNFTSSSC 185
           LGKS SF PAS KV EVIIG+LDTGVWPELESF+D GLGP+PASWKGECEVGKNF SSSC
Sbjct: 121 LGKSASFFPASAKVSEVIIGILDTGVWPELESFSDDGLGPIPASWKGECEVGKNFNSSSC 180

Query: 186 NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGTHTSTTAAGSAVTGANLFGYAAG 245
           NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHG+HTSTTAAGSAVTGANLFG+AAG
Sbjct: 181 NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAG 240

Query: 246 TARGMAAEARVATYKVCWLGGCFGSDILAAMDKAVGDGVNVLSLSLGGSSPDYYRDNVAI 305
           TARGMAAEARVATYKVCWLGGCF SDILAA+DK+V DG N++S+SLGG+S DYYRDNVAI
Sbjct: 241 TARGMAAEARVATYKVCWLGGCFSSDILAAIDKSVEDGCNIVSMSLGGNSADYYRDNVAI 300

Query: 306 GAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKFTGE 365
           GAFSAAAQGVFVSCSAGNGGPSS +LSNVAPWITTVGAGTLDRDFPAYVTLGNGKK TGE
Sbjct: 301 GAFSAAAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGE 360

Query: 366 SLYSGKPLPDSLIPIVYAGNASNSTSGSLCLTSTLIPAKVAGKIVVCDRGGNSRVQKGVV 425
           SLYSGKPLP+SL+PIV A +ASNS+SGSLCL+ TL PAKVAGKIVVCDRGGNSRVQKG+V
Sbjct: 361 SLYSGKPLPNSLLPIVSAASASNSSSGSLCLSGTLNPAKVAGKIVVCDRGGNSRVQKGLV 420

Query: 426 VKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKAGDAIKSYISSDANPTATISTGTT 485
           VKEAGG GMILANTE YGEEQLADAHL+P AAVGQ+AGDAIKSYISSD+NPTATISTGTT
Sbjct: 421 VKEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQEAGDAIKSYISSDSNPTATISTGTT 480

Query: 486 RLGVQPSPVVAAFSSRGPNFLTPQILKPDLIAPGVNILAGWTGGAGPTGSEDDKRRVPFN 545
           RLGVQPSPVVAAFSSRGPN LTPQILKPDLIAPGVNILAGWTGGAGPTG + DKR V FN
Sbjct: 481 RLGVQPSPVVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFN 540

Query: 546 IISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTSYSTYKSGEAIQDISNGLASTP 605
           IISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTT+YSTYK+GE IQDISNG  STP
Sbjct: 541 IISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGETIQDISNGSPSTP 600

Query: 606 FDIGAGHVNPTAALDPGLVYDTTTDDYFAFLCALNYSSLQIKVIAKKDFTCSANKNYRLE 665
           FDIGAGHVNPTAALDPGLVYDTTTDDY AFLCALNYSSLQIKVI+KKDFTC+ NKNY+LE
Sbjct: 601 FDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLE 660

Query: 666 DLNYPSFAVPLETPSTRGGGDAAPTTVKYTRTLTNKGASSTYKATVTSKSPSVKIVVEPE 725
           DLNYPSFAVPLETPST+ G + APTTVKYTRTLTNKGA STYK +VT+K  SVKIVV PE
Sbjct: 661 DLNYPSFAVPLETPSTKRGENVAPTTVKYTRTLTNKGAPSTYKVSVTAKISSVKIVVAPE 720

Query: 726 SLSFAEANEQKSYTVTFIAFPMPSGTVSFARLEWSDGKHVVGSPIAFTWT 776
           SLSF EANEQKSYTVTFIA PMPSG+ SFARLEWSDGKH+VGSPIAFTWT
Sbjct: 721 SLSFTEANEQKSYTVTFIASPMPSGSQSFARLEWSDGKHIVGSPIAFTWT 770

BLAST of Spg025357 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 996.9 bits (2576), Expect = 9.1e-291
Identity = 498/764 (65.18%), Postives = 600/764 (78.53%), Query Frame = 0

Query: 14  WFLLFLISFCFCSFSVALKKNQQLKKKTYIVHMDRTNMPLAFDDHFQWYDSSLKSVSDSA 73
           +FLL  + FC  S S +        + TYIVHM ++ MP +FD H  WYDSSL+S+SDSA
Sbjct: 11  FFLLLCLGFCHVSSSSS-------DQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSA 70

Query: 74  QMLYSYNTVVHGFSTRLTVEEAKLIEKQLGILAVVPEEKYELHTTRTPEFLGLGK-SVSF 133
           ++LY+Y   +HGFSTRLT EEA  +  Q G+++V+PE +YELHTTRTP FLGL + +   
Sbjct: 71  ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL 130

Query: 134 LPASEKVGEVIIGVLDTGVWPELESFNDAGLGPVPASWKGECEVGKNFTSSSCNRKLIGA 193
            P +    +V++GVLDTGVWPE +S++D G GP+P+SWKG CE G NFT+S CNRKLIGA
Sbjct: 131 FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGA 190

Query: 194 RYFSKGYEAAFGPIDESQESKSPRDDDGHGTHTSTTAAGSAVTGANLFGYAAGTARGMAA 253
           R+F++GYE+  GPIDES+ES+SPRDDDGHGTHTS+TAAGS V GA+L GYA+GTARGMA 
Sbjct: 191 RFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAP 250

Query: 254 EARVATYKVCWLGGCFGSDILAAMDKAVGDGVNVLSLSLGGSSPDYYRDNVAIGAFSAAA 313
            ARVA YKVCWLGGCF SDILAA+DKA+ D VNVLS+SLGG   DYYRD VAIGAF+A  
Sbjct: 251 RARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAME 310

Query: 314 QGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKFTGESLYSGKP 373
           +G+ VSCSAGN GPSS SLSNVAPWITTVGAGTLDRDFPA   LGNGK FTG SL+ G+ 
Sbjct: 311 RGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEA 370

Query: 374 LPDSLIPIVYAGNASNSTSGSLCLTSTLIPAKVAGKIVVCDRGGNSRVQKGVVVKEAGGA 433
           LPD L+P +YAGNASN+T+G+LC+T TLIP KV GKIV+CDRG N+RVQKG VVK AGG 
Sbjct: 371 LPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGV 430

Query: 434 GMILANTETYGEEQLADAHLLPAAAVGQKAGDAIKSYISSDANPTATISTGTTRLGVQPS 493
           GMILANT   GEE +ADAHLLPA  VG+KAGD I+ Y+++D NPTA+IS   T +GV+PS
Sbjct: 431 GMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPS 490

Query: 494 PVVAAFSSRGPNFLTPQILKPDLIAPGVNILAGWTGGAGPTGSEDDKRRVPFNIISGTSM 553
           PVVAAFSSRGPN +TP ILKPDLIAPGVNILA WTG AGPTG   D RRV FNIISGTSM
Sbjct: 491 PVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSM 550

Query: 554 SCPHISGLAALLKAAHPDWSPAAIRSALMTTSYSTYKSGEAIQDISNGLASTPFDIGAGH 613
           SCPH+SGLAALLK+ HP+WSPAAIRSALMTT+Y TYK G+ + DI+ G  STPFD GAGH
Sbjct: 551 SCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGH 610

Query: 614 VNPTAALDPGLVYDTTTDDYFAFLCALNYSSLQIKVIAKKDFTCSANKNYRLEDLNYPSF 673
           V+PT A +PGL+YD TT+DY  FLCALNY+S QI+ ++++++TC  +K+Y + DLNYPSF
Sbjct: 611 VSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSF 670

Query: 674 AVPLETPSTRGGGDAAPTTVKYTRTLTNKGASSTYKATVTSKSPSVKIVVEPESLSFAEA 733
           AV ++     G G       KYTRT+T+ G + TY   VTS++  VKI VEP  L+F EA
Sbjct: 671 AVNVD-----GVG-----AYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEA 730

Query: 734 NEQKSYTVTF-IAFPMPSGTVSFARLEWSDGKHVVGSPIAFTWT 776
           NE+KSYTVTF +    PSG+ SF  +EWSDGKHVVGSP+A +WT
Sbjct: 731 NEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISWT 757

BLAST of Spg025357 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 770.4 bits (1988), Expect = 1.4e-222
Identity = 417/741 (56.28%), Postives = 509/741 (68.69%), Query Frame = 0

Query: 39  KKTYIVHMDRTNMPLAFDDHFQWYDSSLKSVSDSAQMLYSYNTVVHGFSTRLTVEEA-KL 98
           KKTYI+ ++ ++ P +F  H  WY S L S S    +LY+Y T  HGFS  L   EA  L
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES---SLLYTYTTSFHGFSAYLDSTEADSL 86

Query: 99  IEKQLGILAVVPEEKYELHTTRTPEFLGLGKSVSFLPASEKVGEVIIGVLDTGVWPELES 158
           +     IL +  +  Y LHTTRTPEFLGL               VIIGVLDTGVWPE  S
Sbjct: 87  LSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPESRS 146

Query: 159 FNDAGLGPVPASWKGECEVGKNFTSSSCNRKLIGARYFSKGYE-AAFGPIDESQESKSPR 218
           F+D  +  +P+ WKGECE G +F S  CN+KLIGAR FSKG++ A+ G     +ES SPR
Sbjct: 147 FDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESVSPR 206

Query: 219 DDDGHGTHTSTTAAGSAVTGANLFGYAAGTARGMAAEARVATYKVCWLGGCFGSDILAAM 278
           D DGHGTHTSTTAAGSAV  A+  GYAAGTARGMA  ARVATYKVCW  GCFGSDILAAM
Sbjct: 207 DVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDILAAM 266

Query: 279 DKAVGDGVNVLSLSLGGSSPDYYRDNVAIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAP 338
           D+A+ DGV+VLSLSLGG S  YYRD +AIGAFSA  +GVFVSCSAGN GP+  S++NVAP
Sbjct: 267 DRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAP 326

Query: 339 WITTVGAGTLDRDFPAYVTLGNGKKFTGESLYSGKPLPDSLIPIVYAGNASNSTSGSLCL 398
           W+ TVGAGTLDRDFPA+  LGNGK+ TG SLYSG  +    + +VY  N  NS+S +LCL
Sbjct: 327 WVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVY--NKGNSSSSNLCL 386

Query: 399 TSTLIPAKVAGKIVVCDRGGNSRVQKGVVVKEAGGAGMILANTETYGEEQLADAHLLPAA 458
             +L  + V GKIVVCDRG N+RV+KG VV++AGG GMI+ANT   GEE +AD+HLLPA 
Sbjct: 387 PGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAI 446

Query: 459 AVGQKAGDAIKSYISSDANPTATISTGTTRLGVQPSPVVAAFSSRGPNFLTPQILKPDLI 518
           AVG+K GD ++ Y+ SD+ PTA +    T L V+PSPVVAAFSSRGPN +TP+ILKPD+I
Sbjct: 447 AVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVI 506

Query: 519 APGVNILAGWTGGAGPTGSEDDKRRVPFNIISGTSMSCPHISGLAALLKAAHPDWSPAAI 578
            PGVNILAGW+   GPTG + D RR  FNI+SGTSMSCPHISGLA LLKAAHP+WSP+AI
Sbjct: 507 GPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAI 566

Query: 579 RSALMTTSYSTYKSGEAIQDISNGLASTPFDIGAGHVNPTAALDPGLVYDTTTDDYFAFL 638
           +SALMTT+Y    +   + D ++   S P+  G+GHV+P  AL PGLVYD +T++Y  FL
Sbjct: 567 KSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFL 626

Query: 639 CALNYSSLQIKVIAKKDFTCSANKNYRLEDLNYPSFAVPLETPSTRGGGDAAPTTVKYTR 698
           C+L+Y+   I  I K+     + K      LNYPSF+V         GG      V+YTR
Sbjct: 627 CSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSVLF-------GG---KRVVRYTR 686

Query: 699 TLTNKG-ASSTYKATVTSKSPSVKIVVEPESLSFAEANEQKSYTVTFIAFPMPSGT--VS 758
            +TN G ASS YK TV   +PSV I V+P  LSF    E+K YTVTF++    S T    
Sbjct: 687 EVTNVGAASSVYKVTVNG-APSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAE 746

Query: 759 FARLEWSDGKHVVGSPIAFTW 775
           F  + WS+ +H V SP+AF+W
Sbjct: 747 FGSITWSNPQHEVRSPVAFSW 751

BLAST of Spg025357 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 751.1 bits (1938), Expect = 8.7e-217
Identity = 405/775 (52.26%), Postives = 515/775 (66.45%), Query Frame = 0

Query: 17  LFLISFCFCSFSVALKKNQQLKKKTYIVHMDRTNMPLAFDDHFQWYDSSLKSVS------ 76
           LF+I      F  A    Q   KKTY++HMD++ MPL + +H QWY S + SV+      
Sbjct: 12  LFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQE 71

Query: 77  ---DSAQMLYSYNTVVHGFSTRLTVEEAKLIEKQLGILAVVPEEKYELHTTRTPEFLGLG 136
              ++ ++LY+Y T  HG + +LT EEA+ +E++ G++AV+PE +YELHTTR+P FLGL 
Sbjct: 72  EEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLE 131

Query: 137 KSVSFLPASEKV--GEVIIGVLDTGVWPELESFNDAGLGPVPASWKGECEVGKNFTSSSC 196
           +  S    +E+V   +V++GVLDTG+WPE ESFND G+ PVPA+W+G CE GK F   +C
Sbjct: 132 RQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNC 191

Query: 197 NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGTHTSTTAAGSAVTGANLFGYAAG 256
           NRK++GAR F +GYEAA G IDE  E KSPRD DGHGTHT+ T AGS V GANLFG+A G
Sbjct: 192 NRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYG 251

Query: 257 TARGMAAEARVATYKVCWLGGCFGSDILAAMDKAVGDGVNVLSLSLGGSSPDYYRDNVAI 316
           TARGMA +ARVA YKVCW+GGCF SDIL+A+D+AV DGV VLS+SLGG    Y RD+++I
Sbjct: 252 TARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSI 311

Query: 317 GAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKFTGE 376
             F A   GVFVSCSAGNGGP   SL+NV+PWITTVGA T+DRDFPA V +G  + F G 
Sbjct: 312 ATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGV 371

Query: 377 SLYSGKPL--PDSLIPIVYAG-NASNSTSGSLCLTSTLIPAKVAGKIVVCDRGGNSRVQK 436
           SLY G+ +   +   P+VY G NAS+    S CL   L    VAGKIV+CDRG   RVQK
Sbjct: 372 SLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQK 431

Query: 437 GVVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKAGDAIKSYISSDANPTATIST 496
           G VVK AGG GM+L NT T GEE +AD+H+LPA AVG+K G  IK Y  +    TA++  
Sbjct: 432 GQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEI 491

Query: 497 GTTRLGVQPSPVVAAFSSRGPNFLTPQILKPDLIAPGVNILAGWTGGAGPTGSEDDKRRV 556
             TR+G++PSPVVAAFSSRGPNFL+ +ILKPDL+APGVNILA WTG   P+    D RRV
Sbjct: 492 LGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRV 551

Query: 557 PFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTSYSTYKSGEAIQDISNGLA 616
            FNI+SGTSMSCPH+SG+AAL+K+ HPDWSPAAI+SALMTT+Y      + + D S    
Sbjct: 552 KFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAP 611

Query: 617 STPFDIGAGHVNPTAALDPGLVYDTTTDDYFAFLCALNYSSLQIKVIAK-KDFTCSANKN 676
           S+P+D GAGH++P  A DPGLVYD    +YF FLC  + S  Q+KV  K  + TC     
Sbjct: 612 SSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLA 671

Query: 677 YRLEDLNYPSFAVPLETPSTRGGGDAAPTTVKYTRTLTNKGAS-STYKATVTSKSPSVKI 736
               +LNYP+ +            +     +   RT+TN G   S+YK +V S      +
Sbjct: 672 KNPGNLNYPAISALFPE-------NTHVKAMTLRRTVTNVGPHISSYKVSV-SPFKGASV 731

Query: 737 VVEPESLSFAEANEQKSYTVTF-IAFPMPSGTVSFARLEWSDGKHVVGSPIAFTW 775
            V+P++L+F   +++ SYTVTF   F M      F  L W    H V SP+  TW
Sbjct: 732 TVQPKTLNFTSKHQKLSYTVTFRTRFRMK--RPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of Spg025357 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 739.6 bits (1908), Expect = 2.6e-213
Identity = 402/782 (51.41%), Postives = 523/782 (66.88%), Query Frame = 0

Query: 10  RLSLWFLLFLISFCFCSFSVALKKNQQLKKKTYIVHMDRTNMPLAFDDHFQWYDSSLKSV 69
           +LSL  + F+     C FS +   +  L  ++YIVH+ R++ P  F  H  W+ S L+S+
Sbjct: 3   KLSLSSIFFVFPLLLCFFSPSSSSSDGL--ESYIVHVQRSHKPSLFSSHNNWHVSLLRSL 62

Query: 70  SDSAQ---MLYSYNTVVHGFSTRLTVEEAKLIEKQLGILAVVPEEKYELHTTRTPEFLGL 129
             S Q   +LYSY+  VHGFS RL+  +   + +   +++V+P++  E+HTT TP FLG 
Sbjct: 63  PSSPQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGF 122

Query: 130 GKSVSFLPASEKVGEVIIGVLDTGVWPELESFNDAGLGPVPASWKGECEVGKNFTSSSCN 189
            ++      S    +VI+GVLDTG+WPE  SF+D+GLGP+P++WKGECE+G +F +SSCN
Sbjct: 123 SQNSGLWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCN 182

Query: 190 RKLIGARYFSKGYEAAFGPIDE--SQESKSPRDDDGHGTHTSTTAAGSAVTGANLFGYAA 249
           RKLIGAR F +GY        +  ++ES+SPRD +GHGTHT++TAAGS V  A+L+ YA 
Sbjct: 183 RKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYAR 242

Query: 250 GTARGMAAEARVATYKVCWLGGCFGSDILAAMDKAVGDGVNVLSLSLG--GSSPDYYRDN 309
           GTA GMA++AR+A YK+CW GGC+ SDILAAMD+AV DGV+V+SLS+G  GS+P+Y+ D+
Sbjct: 243 GTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDS 302

Query: 310 VAIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKF 369
           +AIGAF A   G+ VSCSAGN GP+  + +N+APWI TVGA T+DR+F A    G+GK F
Sbjct: 303 IAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVF 362

Query: 370 TGESLYSGKPLPDSLIPIVYAGNASNSTSGSLCLTSTLIPAKVAGKIVVCDRGGNSRVQK 429
           TG SLY+G+ LPDS + +VY+G+  +     LC    L  + V GKIV+CDRGGN+RV+K
Sbjct: 363 TGTSLYAGESLPDSQLSLVYSGDCGS----RLCYPGKLNSSLVEGKIVLCDRGGNARVEK 422

Query: 430 GVVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKAGDAIKSYISSDANPTATIST 489
           G  VK AGGAGMILANT   GEE  AD+HL+PA  VG KAGD I+ YI +  +PTA IS 
Sbjct: 423 GSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISF 482

Query: 490 GTTRLGVQ-PSPVVAAFSSRGPNFLTPQILKPDLIAPGVNILAGWTGGAGPTGSEDDKRR 549
             T +G   PSP VAAFSSRGPN LTP ILKPD+IAPGVNILAGWTG  GPT  + D RR
Sbjct: 483 LGTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRR 542

Query: 550 VPFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTSYSTYKSGEAIQDISNGL 609
           V FNIISGTSMSCPH+SGLAALL+ AHPDWSPAAI+SAL+TT+Y    SGE I+D++ G 
Sbjct: 543 VQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGK 602

Query: 610 ASTPFDIGAGHVNPTAALDPGLVYDTTTDDYFAFLCALNYSSLQIKVIAKKDF---TCSA 669
           +S  F  GAGHV+P  AL+PGLVYD    +Y AFLCA+ Y    I V  +       C  
Sbjct: 603 SSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACET 662

Query: 670 NKNYRLEDLNYPSFAVPLETPSTRGGGDAAPTTVKYTRTLTNKGASSTYKATVTSKSP-S 729
           +K     DLNYPSF+V   +             VKY R + N G++      V  KSP +
Sbjct: 663 SKLRTAGDLNYPSFSVVFASTG---------EVVKYKRVVKNVGSNVDAVYEVGVKSPAN 722

Query: 730 VKIVVEPESLSFAEANEQKSYTVTFIAFPMPSGTVS-----FARLEWSDGKHVVGSPIAF 775
           V+I V P  L+F++      Y VTF +  +  G  S     F  +EW+DG+HVV SP+A 
Sbjct: 723 VEIDVSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAV 769

BLAST of Spg025357 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 707.2 bits (1824), Expect = 1.4e-203
Identity = 389/777 (50.06%), Postives = 505/777 (64.99%), Query Frame = 0

Query: 14  WFLLFLISFCFCSFSVALKKNQQLKKKTYIVHMDRTNMPLAFDDHFQWYDSSLKSVSDS- 73
           +F  F       S S +   +  L   TYIVH+D    P  F  HF WY SSL S++ S 
Sbjct: 3   FFFYFFFLLTLSSPSSSASSSNSL---TYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSP 62

Query: 74  AQMLYSYNTVVHGFSTRLTVEEAKLIEKQLGILAVVPEEKYELHTTRTPEFLGLGKS--V 133
             ++++Y+TV HGFS RLT ++A  +     +++V+PE+   LHTTR+PEFLGL  +   
Sbjct: 63  PSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKA 122

Query: 134 SFLPASEKVGEVIIGVLDTGVWPELESFNDAGLGPVPASWKGECEVGKNFTSSSCNRKLI 193
             L  S+   +++IGV+DTGVWPE  SF+D GLGPVP  WKG+C   ++F  S+CNRKL+
Sbjct: 123 GLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLV 182

Query: 194 GARYFSKGYEAAFGPIDESQESKSPRDDDGHGTHTSTTAAGSAVTGANLFGYAAGTARGM 253
           GAR+F  GYEA  G ++E+ E +SPRD DGHGTHT++ +AG  V  A+  GYA G A GM
Sbjct: 183 GARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGM 242

Query: 254 AAEARVATYKVCWLGGCFGSDILAAMDKAVGDGVNVLSLSLGGSSPDYYRDNVAIGAFSA 313
           A +AR+A YKVCW  GC+ SDILAA D AV DGV+V+SLS+GG    YY D +AIGAF A
Sbjct: 243 APKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGA 302

Query: 314 AAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKFTGESLYSG 373
             +G+FVS SAGNGGP + +++NVAPW+TTVGAGT+DRDFPA V LGNGK  +G S+Y G
Sbjct: 303 IDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGG 362

Query: 374 KPL-PDSLIPIVYAGN--ASNSTSGSLCLTSTLIPAKVAGKIVVCDRGGNSRVQKGVVVK 433
             L P  + P+VY G+    +  S SLCL  +L P  V GKIV+CDRG NSR  KG +V+
Sbjct: 363 PGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVR 422

Query: 434 EAGGAGMILANTETYGEEQLADAHLLPAAAVGQKAGDAIKSYIS------SDANPTATIS 493
           + GG GMI+AN    GE  +AD H+LPA +VG   GD I+ YIS      S  +PTATI 
Sbjct: 423 KNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIV 482

Query: 494 TGTTRLGVQPSPVVAAFSSRGPNFLTPQILKPDLIAPGVNILAGWTGGAGPTGSEDDKRR 553
              TRLG++P+PVVA+FS+RGPN  TP+ILKPD+IAPG+NILA W    GP+G   D RR
Sbjct: 483 FKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRR 542

Query: 554 VPFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTSYSTYKSGEAIQDISNGL 613
             FNI+SGTSM+CPH+SGLAALLKAAHPDWSPAAIRSAL+TT+Y+   SGE + D S G 
Sbjct: 543 TEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGN 602

Query: 614 ASTPFDIGAGHVNPTAALDPGLVYDTTTDDYFAFLCALNYSSLQIKVIAKKDFTC-SANK 673
            S+  D G+GHV+PT A+DPGLVYD T+ DY  FLC  NY+   I  I ++   C  A +
Sbjct: 603 TSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARR 662

Query: 674 NYRLEDLNYPSFAVPLETPSTRGGGDAAPTTVKYTRTLTNKGASSTYKATVTSKSPSVKI 733
              + +LNYPSF+V  +       G++  +T  + RT+TN G S +             +
Sbjct: 663 AGHVGNLNYPSFSVVFQQ-----YGESKMST-HFIRTVTNVGDSDSVYEIKIRPPRGTTV 722

Query: 734 VVEPESLSFAEANEQKSYTV----TFIAFPMPSGTVSFARLEWSDGKHVVGSPIAFT 774
            VEPE LSF    ++ S+ V    T +     +  V    + WSDGK  V SP+  T
Sbjct: 723 TVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770