Spg025311 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg025311 vs. NCBI nr
Match: XP_023002177.1 (probable carbohydrate esterase At4g34215 [Cucurbita maxima] >XP_023002870.1 probable carbohydrate esterase At4g34215 [Cucurbita maxima] >XP_023002871.1 probable carbohydrate esterase At4g34215 [Cucurbita maxima])

HSP 1 Score: 485.7 bits (1249), Expect = 2.7e-133
Identity = 230/289 (79.58%), Postives = 257/289 (88.93%), Query Frame = 0

Query: 1   MALWRLSILLCMMLFGSSLSGATSPKNIFILAGQSNMAGRGGVEKDQSGKNLVWDGYVPP 60
           M L +LS LLCM+LF  SLS ATSP NIFILAGQSNMAGRGGVE +Q GK L WDG VP 
Sbjct: 1   MILLKLSTLLCMILFHPSLSWATSPTNIFILAGQSNMAGRGGVENNQKGK-LEWDGKVPL 60

Query: 61  ICQPHRSILRLNPKRKWEIAREPIHAGIDLGKTPGIGPAMAIARQLQAKVGKKAGVVGLV 120
            CQ   SILRLNP R+WEIA+EP+H GID+GKTPGIGP +  A Q +AK G+KAG+VGLV
Sbjct: 61  ECQSDPSILRLNPARQWEIAQEPLHLGIDIGKTPGIGPGIPFAHQFKAKAGQKAGIVGLV 120

Query: 121 PCARGGTLIEQWIKNPSNPNATFYQNFIERIKASDRKGGVVRALFWYQGESDAAMSDTAH 180
           PCARGGTLIEQWIKNPSNP+ATFYQNFIERIK S+++GGVVRALFWYQGESDAAMSDTAH
Sbjct: 121 PCARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMSDTAH 180

Query: 181 RYKDNLKKFFIDIRNDIKPRFLPIIIVKVALYDFFMKHDTHDLPTVRAAQEALQHELPDV 240
           RYKDNLKKF  DIRNDIKPRFLP+IIVK+++YDFFMKHDTHDLP VRAA++A+Q ELPD+
Sbjct: 181 RYKDNLKKFITDIRNDIKPRFLPVIIVKISMYDFFMKHDTHDLPAVRAAEDAVQKELPDI 240

Query: 241 VTIDSWKLPINFTTFEGFNLDHGHFNTATELALGKWLADTYLSHYGHLL 290
           +TIDSW+LPINFTTFEGF LDHGHFNTATE+ALGKWLADTYL+HY HLL
Sbjct: 241 ITIDSWELPINFTTFEGFCLDHGHFNTATEIALGKWLADTYLAHYSHLL 288

BLAST of Spg025311 vs. NCBI nr
Match: XP_023536885.1 (probable carbohydrate esterase At4g34215 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023536886.1 probable carbohydrate esterase At4g34215 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 477.2 bits (1227), Expect = 9.7e-131
Identity = 225/289 (77.85%), Postives = 254/289 (87.89%), Query Frame = 0

Query: 1   MALWRLSILLCMMLFGSSLSGATSPKNIFILAGQSNMAGRGGVEKDQSGKNLVWDGYVPP 60
           M L +LSILLCM+LF  SLSGATSP NIFILAGQSNMAGRGGVEK Q+GK LVWDG VP 
Sbjct: 1   MVLLKLSILLCMILFSPSLSGATSPTNIFILAGQSNMAGRGGVEKIQNGK-LVWDGKVPL 60

Query: 61  ICQPHRSILRLNPKRKWEIAREPIHAGIDLGKTPGIGPAMAIARQLQAKVGKKAGVVGLV 120
            CQ   SILRLNP+R+WEIA EP+H GID+  TPGIGP +  A Q + K G+KAG VGLV
Sbjct: 61  ECQSDPSILRLNPERQWEIAHEPLHLGIDISHTPGIGPGIPFAHQFKEKAGQKAGTVGLV 120

Query: 121 PCARGGTLIEQWIKNPSNPNATFYQNFIERIKASDRKGGVVRALFWYQGESDAAMSDTAH 180
           PCARGGTLIEQWIKNPSNP+ATFYQNFIERIK S+++GGVVRALFWYQGESDAAM+DTA 
Sbjct: 121 PCARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQ 180

Query: 181 RYKDNLKKFFIDIRNDIKPRFLPIIIVKVALYDFFMKHDTHDLPTVRAAQEALQHELPDV 240
           RYKDNLKKF  DIRNDIKPRFLP+IIVK+++YDFFMKHDTH+LP VRAA++A+Q ELPD+
Sbjct: 181 RYKDNLKKFITDIRNDIKPRFLPVIIVKISMYDFFMKHDTHNLPAVRAAEDAVQKELPDI 240

Query: 241 VTIDSWKLPINFTTFEGFNLDHGHFNTATELALGKWLADTYLSHYGHLL 290
           +TIDSW+LPIN TTFEGF+ DHGHFNT TE+ LGKWLADTYL+HYGHLL
Sbjct: 241 ITIDSWELPINLTTFEGFSWDHGHFNTETEIVLGKWLADTYLAHYGHLL 288

BLAST of Spg025311 vs. NCBI nr
Match: XP_022973316.1 (probable carbohydrate esterase At4g34215 [Cucurbita maxima] >XP_022973317.1 probable carbohydrate esterase At4g34215 [Cucurbita maxima])

HSP 1 Score: 474.2 bits (1219), Expect = 8.2e-130
Identity = 225/291 (77.32%), Postives = 256/291 (87.97%), Query Frame = 0

Query: 1   MALWRLSILLCMMLFGSSLSGATSPKNIFILAGQSNMAGRGGVEKDQSGKNLVWDGYVPP 60
           M L +LSIL+C +L   SLSGATSP NIFILAGQSNMAGRGGVEK  +G+ LVWDG VP 
Sbjct: 1   MVLLKLSILMCTILLSPSLSGATSPTNIFILAGQSNMAGRGGVEKTPTGE-LVWDGKVPS 60

Query: 61  ICQPHRSILRLNPKRKWEIAREPIHAGIDLG--KTPGIGPAMAIARQLQAKVGKKAGVVG 120
            CQ   SILR NP+R+WEIA EP+H GID+G  KTPGIGP +  A QL+ K G+KAG+VG
Sbjct: 61  ECQSDPSILRFNPERQWEIAHEPLHLGIDVGKTKTPGIGPGIPFAHQLKEKAGQKAGIVG 120

Query: 121 LVPCARGGTLIEQWIKNPSNPNATFYQNFIERIKASDRKGGVVRALFWYQGESDAAMSDT 180
           LVPCARGGTLIEQWIKNPSNP+ATFYQNFIERIK S+++GGVVRALFWYQGESDAAM+DT
Sbjct: 121 LVPCARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDT 180

Query: 181 AHRYKDNLKKFFIDIRNDIKPRFLPIIIVKVALYDFFMKHDTHDLPTVRAAQEALQHELP 240
           A RYKDNLKKF  DIRNDIKPRFLP+IIVK+ALYDFFMKHDTH+LP VRAA++A+Q ELP
Sbjct: 181 AQRYKDNLKKFITDIRNDIKPRFLPVIIVKIALYDFFMKHDTHNLPAVRAAEDAVQKELP 240

Query: 241 DVVTIDSWKLPINFTTFEGFNLDHGHFNTATELALGKWLADTYLSHYGHLL 290
           D++TIDSW+LP+N TTFEGF+ DHGHFNTATE+ALGKWLADTYL+HYGHLL
Sbjct: 241 DIITIDSWELPMNLTTFEGFSWDHGHFNTATEIALGKWLADTYLAHYGHLL 290

BLAST of Spg025311 vs. NCBI nr
Match: XP_022131651.1 (probable carbohydrate esterase At4g34215 [Momordica charantia])

HSP 1 Score: 472.6 bits (1215), Expect = 2.4e-129
Identity = 230/289 (79.58%), Postives = 258/289 (89.27%), Query Frame = 0

Query: 1   MALWRLSILLCMMLFGSSLSGATSPKNIFILAGQSNMAGRGGVEKDQSGKNLVWDGYVPP 60
           MAL RLSI+LCMMLFG SLSGATSPKNIFILAGQSNMAGRGGVEK+++G +L WDGYVPP
Sbjct: 1   MALLRLSIMLCMMLFGPSLSGATSPKNIFILAGQSNMAGRGGVEKNRTG-DLEWDGYVPP 60

Query: 61  ICQPHRSILRLNPKRKWEIAREPIHAGIDLGKTPGIGPAMAIARQLQAKVGKKAGVVGLV 120
             QP  SILRLNP+R+WE+AREP+H GID+GKT G+GPA+A A QLQAK G K G VGLV
Sbjct: 61  ESQPDPSILRLNPERQWEVAREPVHRGIDIGKTVGVGPAIAFAHQLQAKGGSKVGSVGLV 120

Query: 121 PCARGGTLIEQWIKNPSNPNATFYQNFIERIKASDRKGGVVRALFWYQGESDAAMSDTAH 180
           PCARGGTLIEQW+KNPSNPNATFY+NFIERI+ASDR+GGVVRALFW QGESDAA SDTA 
Sbjct: 121 PCARGGTLIEQWVKNPSNPNATFYKNFIERIQASDREGGVVRALFWLQGESDAASSDTAE 180

Query: 181 RYKDNLKKFFIDIRNDIKPRFLPIIIVKVALYDFFMKHDTHDLPTVRAAQEALQHELPDV 240
           RYK+NLKKFF DIRNDIKPR LPII+VK+A+YD FMKHDTHDLP VRAA++A+Q ELP+V
Sbjct: 181 RYKNNLKKFFTDIRNDIKPRVLPIILVKIAVYDTFMKHDTHDLPAVRAAEDAVQRELPNV 240

Query: 241 VTIDSWKLPINFTTFEGFNLDHGHFNTATELALGKWLADTYLSHYGHLL 290
           VTID+ KL +N TT EGFNLD GHFN  TE+ALGKWLADTYLS+YGHLL
Sbjct: 241 VTIDALKL-VNTTTAEGFNLDRGHFNIKTEIALGKWLADTYLSNYGHLL 287

BLAST of Spg025311 vs. NCBI nr
Match: XP_022951659.1 (probable carbohydrate esterase At4g34215 [Cucurbita moschata] >XP_022951660.1 probable carbohydrate esterase At4g34215 [Cucurbita moschata])

HSP 1 Score: 472.6 bits (1215), Expect = 2.4e-129
Identity = 224/289 (77.51%), Postives = 255/289 (88.24%), Query Frame = 0

Query: 1   MALWRLSILLCMMLFGSSLSGATSPKNIFILAGQSNMAGRGGVEKDQSGKNLVWDGYVPP 60
           M L +LSILLCM+LF  SLSGATSP NIFILAGQSNMAGRGGVEK Q+GK LVWDG VP 
Sbjct: 1   MVLLKLSILLCMILFNPSLSGATSPTNIFILAGQSNMAGRGGVEKIQNGK-LVWDGKVPL 60

Query: 61  ICQPHRSILRLNPKRKWEIAREPIHAGIDLGKTPGIGPAMAIARQLQAKVGKKAGVVGLV 120
            CQ   SILRLNP+R+WEIA EP+H GID+  TPGIGP +  A Q + K G+KAG+VGLV
Sbjct: 61  ECQFDPSILRLNPERQWEIAHEPLHLGIDISHTPGIGPGIPFAHQFKEKAGQKAGIVGLV 120

Query: 121 PCARGGTLIEQWIKNPSNPNATFYQNFIERIKASDRKGGVVRALFWYQGESDAAMSDTAH 180
           PCARGGTLIEQWIKNPSNP+ATFYQNFIERIK S+++GGVVRALFWYQGESDAAM+DTA 
Sbjct: 121 PCARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQ 180

Query: 181 RYKDNLKKFFIDIRNDIKPRFLPIIIVKVALYDFFMKHDTHDLPTVRAAQEALQHELPDV 240
           RYKDNLKKF  DIRNDIKPRFLP+IIVK++LYDFFMKHDTH+LP VRAA++A+Q ELPD+
Sbjct: 181 RYKDNLKKFITDIRNDIKPRFLPVIIVKISLYDFFMKHDTHNLPAVRAAEDAVQKELPDI 240

Query: 241 VTIDSWKLPINFTTFEGFNLDHGHFNTATELALGKWLADTYLSHYGHLL 290
           +TIDS +LP+NFTTFEGF+ DHGHFNT TE+ LGKWLA+TYL+HYGHLL
Sbjct: 241 ITIDSRELPVNFTTFEGFSWDHGHFNTETEIVLGKWLANTYLAHYGHLL 288

BLAST of Spg025311 vs. ExPASy Swiss-Prot
Match: Q8L9J9 (Probable carbohydrate esterase At4g34215 OS=Arabidopsis thaliana OX=3702 GN=At4g34215 PE=1 SV=2)

HSP 1 Score: 188.3 bits (477), Expect = 1.2e-46
Identity = 103/261 (39.46%), Postives = 148/261 (56.70%), Query Frame = 0

Query: 25  PKNIFILAGQSNMAGRGGVEKDQSGKNLVWDGYVPPICQPHRSILRLNPKRKWEIAREPI 84
           P  IFIL+GQSNMAGRGGV KD      VWD  +PP C P+ SILRL+   +WE A EP+
Sbjct: 21  PNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEEAHEPL 80

Query: 85  HAGIDLGKTPGIGPAMAIARQLQAKVGKKAGVVGLVPCARGGTLIEQWIKNPSNPNATFY 144
           H  ID GK  G+GP MA A  ++ ++   + V+GLVPCA GGT I++W +      +  Y
Sbjct: 81  HVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWER-----GSHLY 140

Query: 145 QNFIERIKASDRKGGVVRALFWYQGESDAAMSDTAHRYKDNLKKFFIDIRNDIKPRFLPI 204
           +  ++R + S + GG ++A+ WYQGESD      A  Y +N+ +   ++R+D+    LPI
Sbjct: 141 ERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLPI 200

Query: 205 IIVKVALYDFFMKHDTHDLPTVRAAQEALQHELPDVVTIDSWKLPINFTTFEGFNLDHGH 264
           I V +A    +       +  VR AQ  L  +L +VV +D+  LP+          D+ H
Sbjct: 201 IQVAIASGGGY-------IDKVREAQLGL--KLSNVVCVDAKGLPL--------KSDNLH 259

Query: 265 FNTATELALGKWLADTYLSHY 286
             T  ++ LG  LA  YLS++
Sbjct: 261 LTTEAQVQLGLSLAQAYLSNF 259

BLAST of Spg025311 vs. ExPASy TrEMBL
Match: A0A6J1KIR8 (probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC111496116 PE=4 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 1.3e-133
Identity = 230/289 (79.58%), Postives = 257/289 (88.93%), Query Frame = 0

Query: 1   MALWRLSILLCMMLFGSSLSGATSPKNIFILAGQSNMAGRGGVEKDQSGKNLVWDGYVPP 60
           M L +LS LLCM+LF  SLS ATSP NIFILAGQSNMAGRGGVE +Q GK L WDG VP 
Sbjct: 1   MILLKLSTLLCMILFHPSLSWATSPTNIFILAGQSNMAGRGGVENNQKGK-LEWDGKVPL 60

Query: 61  ICQPHRSILRLNPKRKWEIAREPIHAGIDLGKTPGIGPAMAIARQLQAKVGKKAGVVGLV 120
            CQ   SILRLNP R+WEIA+EP+H GID+GKTPGIGP +  A Q +AK G+KAG+VGLV
Sbjct: 61  ECQSDPSILRLNPARQWEIAQEPLHLGIDIGKTPGIGPGIPFAHQFKAKAGQKAGIVGLV 120

Query: 121 PCARGGTLIEQWIKNPSNPNATFYQNFIERIKASDRKGGVVRALFWYQGESDAAMSDTAH 180
           PCARGGTLIEQWIKNPSNP+ATFYQNFIERIK S+++GGVVRALFWYQGESDAAMSDTAH
Sbjct: 121 PCARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMSDTAH 180

Query: 181 RYKDNLKKFFIDIRNDIKPRFLPIIIVKVALYDFFMKHDTHDLPTVRAAQEALQHELPDV 240
           RYKDNLKKF  DIRNDIKPRFLP+IIVK+++YDFFMKHDTHDLP VRAA++A+Q ELPD+
Sbjct: 181 RYKDNLKKFITDIRNDIKPRFLPVIIVKISMYDFFMKHDTHDLPAVRAAEDAVQKELPDI 240

Query: 241 VTIDSWKLPINFTTFEGFNLDHGHFNTATELALGKWLADTYLSHYGHLL 290
           +TIDSW+LPINFTTFEGF LDHGHFNTATE+ALGKWLADTYL+HY HLL
Sbjct: 241 ITIDSWELPINFTTFEGFCLDHGHFNTATEIALGKWLADTYLAHYSHLL 288

BLAST of Spg025311 vs. ExPASy TrEMBL
Match: A0A6J1I774 (probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC111471873 PE=4 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 4.0e-130
Identity = 225/291 (77.32%), Postives = 256/291 (87.97%), Query Frame = 0

Query: 1   MALWRLSILLCMMLFGSSLSGATSPKNIFILAGQSNMAGRGGVEKDQSGKNLVWDGYVPP 60
           M L +LSIL+C +L   SLSGATSP NIFILAGQSNMAGRGGVEK  +G+ LVWDG VP 
Sbjct: 1   MVLLKLSILMCTILLSPSLSGATSPTNIFILAGQSNMAGRGGVEKTPTGE-LVWDGKVPS 60

Query: 61  ICQPHRSILRLNPKRKWEIAREPIHAGIDLG--KTPGIGPAMAIARQLQAKVGKKAGVVG 120
            CQ   SILR NP+R+WEIA EP+H GID+G  KTPGIGP +  A QL+ K G+KAG+VG
Sbjct: 61  ECQSDPSILRFNPERQWEIAHEPLHLGIDVGKTKTPGIGPGIPFAHQLKEKAGQKAGIVG 120

Query: 121 LVPCARGGTLIEQWIKNPSNPNATFYQNFIERIKASDRKGGVVRALFWYQGESDAAMSDT 180
           LVPCARGGTLIEQWIKNPSNP+ATFYQNFIERIK S+++GGVVRALFWYQGESDAAM+DT
Sbjct: 121 LVPCARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDT 180

Query: 181 AHRYKDNLKKFFIDIRNDIKPRFLPIIIVKVALYDFFMKHDTHDLPTVRAAQEALQHELP 240
           A RYKDNLKKF  DIRNDIKPRFLP+IIVK+ALYDFFMKHDTH+LP VRAA++A+Q ELP
Sbjct: 181 AQRYKDNLKKFITDIRNDIKPRFLPVIIVKIALYDFFMKHDTHNLPAVRAAEDAVQKELP 240

Query: 241 DVVTIDSWKLPINFTTFEGFNLDHGHFNTATELALGKWLADTYLSHYGHLL 290
           D++TIDSW+LP+N TTFEGF+ DHGHFNTATE+ALGKWLADTYL+HYGHLL
Sbjct: 241 DIITIDSWELPMNLTTFEGFSWDHGHFNTATEIALGKWLADTYLAHYGHLL 290

BLAST of Spg025311 vs. ExPASy TrEMBL
Match: A0A6J1GJF6 (probable carbohydrate esterase At4g34215 OS=Cucurbita moschata OX=3662 GN=LOC111454406 PE=4 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 1.2e-129
Identity = 224/289 (77.51%), Postives = 255/289 (88.24%), Query Frame = 0

Query: 1   MALWRLSILLCMMLFGSSLSGATSPKNIFILAGQSNMAGRGGVEKDQSGKNLVWDGYVPP 60
           M L +LSILLCM+LF  SLSGATSP NIFILAGQSNMAGRGGVEK Q+GK LVWDG VP 
Sbjct: 1   MVLLKLSILLCMILFNPSLSGATSPTNIFILAGQSNMAGRGGVEKIQNGK-LVWDGKVPL 60

Query: 61  ICQPHRSILRLNPKRKWEIAREPIHAGIDLGKTPGIGPAMAIARQLQAKVGKKAGVVGLV 120
            CQ   SILRLNP+R+WEIA EP+H GID+  TPGIGP +  A Q + K G+KAG+VGLV
Sbjct: 61  ECQFDPSILRLNPERQWEIAHEPLHLGIDISHTPGIGPGIPFAHQFKEKAGQKAGIVGLV 120

Query: 121 PCARGGTLIEQWIKNPSNPNATFYQNFIERIKASDRKGGVVRALFWYQGESDAAMSDTAH 180
           PCARGGTLIEQWIKNPSNP+ATFYQNFIERIK S+++GGVVRALFWYQGESDAAM+DTA 
Sbjct: 121 PCARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQ 180

Query: 181 RYKDNLKKFFIDIRNDIKPRFLPIIIVKVALYDFFMKHDTHDLPTVRAAQEALQHELPDV 240
           RYKDNLKKF  DIRNDIKPRFLP+IIVK++LYDFFMKHDTH+LP VRAA++A+Q ELPD+
Sbjct: 181 RYKDNLKKFITDIRNDIKPRFLPVIIVKISLYDFFMKHDTHNLPAVRAAEDAVQKELPDI 240

Query: 241 VTIDSWKLPINFTTFEGFNLDHGHFNTATELALGKWLADTYLSHYGHLL 290
           +TIDS +LP+NFTTFEGF+ DHGHFNT TE+ LGKWLA+TYL+HYGHLL
Sbjct: 241 ITIDSRELPVNFTTFEGFSWDHGHFNTETEIVLGKWLANTYLAHYGHLL 288

BLAST of Spg025311 vs. ExPASy TrEMBL
Match: A0A6J1BQ38 (probable carbohydrate esterase At4g34215 OS=Momordica charantia OX=3673 GN=LOC111004778 PE=4 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 1.2e-129
Identity = 230/289 (79.58%), Postives = 258/289 (89.27%), Query Frame = 0

Query: 1   MALWRLSILLCMMLFGSSLSGATSPKNIFILAGQSNMAGRGGVEKDQSGKNLVWDGYVPP 60
           MAL RLSI+LCMMLFG SLSGATSPKNIFILAGQSNMAGRGGVEK+++G +L WDGYVPP
Sbjct: 1   MALLRLSIMLCMMLFGPSLSGATSPKNIFILAGQSNMAGRGGVEKNRTG-DLEWDGYVPP 60

Query: 61  ICQPHRSILRLNPKRKWEIAREPIHAGIDLGKTPGIGPAMAIARQLQAKVGKKAGVVGLV 120
             QP  SILRLNP+R+WE+AREP+H GID+GKT G+GPA+A A QLQAK G K G VGLV
Sbjct: 61  ESQPDPSILRLNPERQWEVAREPVHRGIDIGKTVGVGPAIAFAHQLQAKGGSKVGSVGLV 120

Query: 121 PCARGGTLIEQWIKNPSNPNATFYQNFIERIKASDRKGGVVRALFWYQGESDAAMSDTAH 180
           PCARGGTLIEQW+KNPSNPNATFY+NFIERI+ASDR+GGVVRALFW QGESDAA SDTA 
Sbjct: 121 PCARGGTLIEQWVKNPSNPNATFYKNFIERIQASDREGGVVRALFWLQGESDAASSDTAE 180

Query: 181 RYKDNLKKFFIDIRNDIKPRFLPIIIVKVALYDFFMKHDTHDLPTVRAAQEALQHELPDV 240
           RYK+NLKKFF DIRNDIKPR LPII+VK+A+YD FMKHDTHDLP VRAA++A+Q ELP+V
Sbjct: 181 RYKNNLKKFFTDIRNDIKPRVLPIILVKIAVYDTFMKHDTHDLPAVRAAEDAVQRELPNV 240

Query: 241 VTIDSWKLPINFTTFEGFNLDHGHFNTATELALGKWLADTYLSHYGHLL 290
           VTID+ KL +N TT EGFNLD GHFN  TE+ALGKWLADTYLS+YGHLL
Sbjct: 241 VTIDALKL-VNTTTAEGFNLDRGHFNIKTEIALGKWLADTYLSNYGHLL 287

BLAST of Spg025311 vs. ExPASy TrEMBL
Match: A0A6J1GIT2 (probable carbohydrate esterase At4g34215 OS=Cucurbita moschata OX=3662 GN=LOC111454649 PE=4 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 1.7e-128
Identity = 221/289 (76.47%), Postives = 254/289 (87.89%), Query Frame = 0

Query: 1   MALWRLSILLCMMLFGSSLSGATSPKNIFILAGQSNMAGRGGVEKDQSGKNLVWDGYVPP 60
           M L +LS+LLCM+LF  SLSGATSP NIFILAGQSNMAGRGGVEK Q+GK LVWDG VP 
Sbjct: 1   MVLLQLSVLLCMILFSPSLSGATSPTNIFILAGQSNMAGRGGVEKIQNGK-LVWDGKVPL 60

Query: 61  ICQPHRSILRLNPKRKWEIAREPIHAGIDLGKTPGIGPAMAIARQLQAKVGKKAGVVGLV 120
            C  + SILRLNP+R+WEIA EP+H GID+  TPGIGP +  A Q + K G+KAG+VGLV
Sbjct: 61  ECLSNPSILRLNPERQWEIAHEPLHLGIDISHTPGIGPGIPFAHQFKEKAGQKAGIVGLV 120

Query: 121 PCARGGTLIEQWIKNPSNPNATFYQNFIERIKASDRKGGVVRALFWYQGESDAAMSDTAH 180
           PCARGGTLIEQW+KNPSNP+ATFYQNFIERIK S+++GGVVRALFWYQGESDAAM+DTA 
Sbjct: 121 PCARGGTLIEQWMKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQ 180

Query: 181 RYKDNLKKFFIDIRNDIKPRFLPIIIVKVALYDFFMKHDTHDLPTVRAAQEALQHELPDV 240
           RYKDNLKKF  DIRNDIKPRFLP+IIVK++LYDFFMKHDTH+LP VRAA++A+Q ELPD+
Sbjct: 181 RYKDNLKKFITDIRNDIKPRFLPVIIVKISLYDFFMKHDTHNLPAVRAAEDAVQKELPDI 240

Query: 241 VTIDSWKLPINFTTFEGFNLDHGHFNTATELALGKWLADTYLSHYGHLL 290
           +TIDS +LP+NFTTFEGF+ D GHFNT TE+ LGKWLADTYL+HYGHLL
Sbjct: 241 ITIDSRELPVNFTTFEGFSWDRGHFNTETEIVLGKWLADTYLAHYGHLL 288

BLAST of Spg025311 vs. TAIR 10
Match: AT4G34215.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 188.3 bits (477), Expect = 8.4e-48
Identity = 103/261 (39.46%), Postives = 148/261 (56.70%), Query Frame = 0

Query: 25  PKNIFILAGQSNMAGRGGVEKDQSGKNLVWDGYVPPICQPHRSILRLNPKRKWEIAREPI 84
           P  IFIL+GQSNMAGRGGV KD      VWD  +PP C P+ SILRL+   +WE A EP+
Sbjct: 21  PNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEEAHEPL 80

Query: 85  HAGIDLGKTPGIGPAMAIARQLQAKVGKKAGVVGLVPCARGGTLIEQWIKNPSNPNATFY 144
           H  ID GK  G+GP MA A  ++ ++   + V+GLVPCA GGT I++W +      +  Y
Sbjct: 81  HVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWER-----GSHLY 140

Query: 145 QNFIERIKASDRKGGVVRALFWYQGESDAAMSDTAHRYKDNLKKFFIDIRNDIKPRFLPI 204
           +  ++R + S + GG ++A+ WYQGESD      A  Y +N+ +   ++R+D+    LPI
Sbjct: 141 ERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLPI 200

Query: 205 IIVKVALYDFFMKHDTHDLPTVRAAQEALQHELPDVVTIDSWKLPINFTTFEGFNLDHGH 264
           I V +A    +       +  VR AQ  L  +L +VV +D+  LP+          D+ H
Sbjct: 201 IQVAIASGGGY-------IDKVREAQLGL--KLSNVVCVDAKGLPL--------KSDNLH 259

Query: 265 FNTATELALGKWLADTYLSHY 286
             T  ++ LG  LA  YLS++
Sbjct: 261 LTTEAQVQLGLSLAQAYLSNF 259

BLAST of Spg025311 vs. TAIR 10
Match: AT4G34215.2 (Domain of unknown function (DUF303) )

HSP 1 Score: 188.3 bits (477), Expect = 8.4e-48
Identity = 103/261 (39.46%), Postives = 148/261 (56.70%), Query Frame = 0

Query: 25  PKNIFILAGQSNMAGRGGVEKDQSGKNLVWDGYVPPICQPHRSILRLNPKRKWEIAREPI 84
           P  IFIL+GQSNMAGRGGV KD      VWD  +PP C P+ SILRL+   +WE A EP+
Sbjct: 21  PNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEEAHEPL 80

Query: 85  HAGIDLGKTPGIGPAMAIARQLQAKVGKKAGVVGLVPCARGGTLIEQWIKNPSNPNATFY 144
           H  ID GK  G+GP MA A  ++ ++   + V+GLVPCA GGT I++W +      +  Y
Sbjct: 81  HVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWER-----GSHLY 140

Query: 145 QNFIERIKASDRKGGVVRALFWYQGESDAAMSDTAHRYKDNLKKFFIDIRNDIKPRFLPI 204
           +  ++R + S + GG ++A+ WYQGESD      A  Y +N+ +   ++R+D+    LPI
Sbjct: 141 ERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLPI 200

Query: 205 IIVKVALYDFFMKHDTHDLPTVRAAQEALQHELPDVVTIDSWKLPINFTTFEGFNLDHGH 264
           I V +A    +       +  VR AQ  L  +L +VV +D+  LP+          D+ H
Sbjct: 201 IQVAIASGGGY-------IDKVREAQLGL--KLSNVVCVDAKGLPL--------KSDNLH 259

Query: 265 FNTATELALGKWLADTYLSHY 286
             T  ++ LG  LA  YLS++
Sbjct: 261 LTTEAQVQLGLSLAQAYLSNF 259

BLAST of Spg025311 vs. TAIR 10
Match: AT3G53010.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 181.0 bits (458), Expect = 1.3e-45
Identity = 107/260 (41.15%), Postives = 149/260 (57.31%), Query Frame = 0

Query: 27  NIFILAGQSNMAGRGGVEKDQSGKNLVWDGYVPPICQPHRSILRLNPKRKWEIAREPIHA 86
           +IFILAGQSNMAGRGGV  D +    VWDG +PP C+ + SILRL  K +W+ A+EP+H 
Sbjct: 30  SIFILAGQSNMAGRGGVYNDTATNTTVWDGVIPPECRSNPSILRLTSKLEWKEAKEPLHV 89

Query: 87  GIDLGKTPGIGPAMAIARQLQAKVGKKAGVVGLVPCARGGTLIEQWIKNPSNPNATFYQN 146
            ID+ KT G+GP M  A     +V  + G VGLVPC+ GGT + QW K         Y+ 
Sbjct: 90  DIDINKTNGVGPGMPFAN----RVVNRFGQVGLVPCSIGGTKLSQWQK-----GEFLYEE 149

Query: 147 FIERIKA--SDRKGGVVRALFWYQGESDAAMSDTAHRYKDNLKKFFIDIRNDIKPRFLPI 206
            ++R KA  +   GG  RA+ WYQGESD      A  YK  L KFF D+RND++   LPI
Sbjct: 150 TVKRAKAAMASGGGGSYRAVLWYQGESDTVDMVDASVYKKRLVKFFSDLRNDLQHPNLPI 209

Query: 207 IIVKVALYDFFMKHDTHDLPTVRAAQEA-LQHELPDVVTIDSWKLPINFTTFEGFNLDHG 266
           I V +A         T   P + A ++A L+ +L +V  +D+  LP+          D  
Sbjct: 210 IQVALA---------TGAGPYLDAVRKAQLKTDLENVYCVDARGLPL--------EPDGL 263

Query: 267 HFNTATELALGKWLADTYLS 284
           H  T++++ LG  +A+++L+
Sbjct: 270 HLTTSSQVQLGHMIAESFLA 263