Spg025305 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg025305 vs. NCBI nr
Match: XP_022131651.1 (probable carbohydrate esterase At4g34215 [Momordica charantia])

HSP 1 Score: 505.8 bits (1301), Expect = 2.5e-139
Identity = 246/287 (85.71%), Postives = 268/287 (93.38%), Query Frame = 0

Query: 1   MALLKLSILLCTMLFGLSLSGATSPQNIFILAGQSNMAGRGGVEKDHSGVLVWDGYVPPE 60
           MALL+LSI+LC MLFG SLSGATSP+NIFILAGQSNMAGRGGVEK+ +G L WDGYVPPE
Sbjct: 1   MALLRLSIMLCMMLFGPSLSGATSPKNIFILAGQSNMAGRGGVEKNRTGDLEWDGYVPPE 60

Query: 61  AQPDPSILRLSPERQWEIALEPVHEGIDINKTVGVGSAMAFAHHLQAKAGAKAGVVGLVP 120
           +QPDPSILRL+PERQWE+A EPVH GIDI KTVGVG A+AFAH LQAK G+K G VGLVP
Sbjct: 61  SQPDPSILRLNPERQWEVAREPVHRGIDIGKTVGVGPAIAFAHQLQAKGGSKVGSVGLVP 120

Query: 121 CARGGTLIEQWIKNPNNPNATFYQNFIERIKASDKKGGVVRALFWLQGESDAAASDTASR 180
           CARGGTLIEQW+KNP+NPNATFY+NFIERI+ASD++GGVVRALFWLQGESDAA+SDTA R
Sbjct: 121 CARGGTLIEQWVKNPSNPNATFYKNFIERIQASDREGGVVRALFWLQGESDAASSDTAER 180

Query: 181 YKNNLKKFFTDIRNDIKPRFLPIILVKIAVYDTFLKHDTHDLPSVRAAEDAVQRELPDVV 240
           YKNNLKKFFTDIRNDIKPR LPIILVKIAVYDTF+KHDTHDLP+VRAAEDAVQRELP+VV
Sbjct: 181 YKNNLKKFFTDIRNDIKPRVLPIILVKIAVYDTFMKHDTHDLPAVRAAEDAVQRELPNVV 240

Query: 241 TIDSLKLVNTRTHEGFNQDHGHFNIKTEIALGKWLADTYLSHYGHLL 288
           TID+LKLVNT T EGFN D GHFNIKTEIALGKWLADTYLS+YGHLL
Sbjct: 241 TIDALKLVNTTTAEGFNLDRGHFNIKTEIALGKWLADTYLSNYGHLL 287

BLAST of Spg025305 vs. NCBI nr
Match: XP_022158585.1 (probable carbohydrate esterase At4g34215 [Momordica charantia])

HSP 1 Score: 499.2 bits (1284), Expect = 2.4e-137
Identity = 243/287 (84.67%), Postives = 268/287 (93.38%), Query Frame = 0

Query: 1   MALLKLSILLCTMLFGLSLSGATSPQNIFILAGQSNMAGRGGVEKDHSGVLVWDGYVPPE 60
           MALL+LSILLC +L G SLSGATSP+NIFILAGQSNMAGRGGVEKD SG LVWDGYVPPE
Sbjct: 1   MALLRLSILLCMILCGPSLSGATSPKNIFILAGQSNMAGRGGVEKDRSGNLVWDGYVPPE 60

Query: 61  AQPDPSILRLSPERQWEIALEPVHEGIDINKTVGVGSAMAFAHHLQAKAGAKAGVVGLVP 120
           AQPDPSILRL+PERQWE+A EPVHEGIDINKTVGVGSA+AFAH LQAK G+K G VGLVP
Sbjct: 61  AQPDPSILRLNPERQWEVAREPVHEGIDINKTVGVGSAIAFAHQLQAKGGSKVGSVGLVP 120

Query: 121 CARGGTLIEQWIKNPNNPNATFYQNFIERIKASDKKGGVVRALFWLQGESDAAASDTASR 180
           CARGGTLIEQW+KNP+N +ATFY+NFIERI+ASD++GGVVRALFWLQGESDAA+ DTASR
Sbjct: 121 CARGGTLIEQWVKNPSNTSATFYKNFIERIQASDREGGVVRALFWLQGESDAASRDTASR 180

Query: 181 YKNNLKKFFTDIRNDIKPRFLPIILVKIAVYDTFLKHDTHDLPSVRAAEDAVQRELPDVV 240
           YKNNLKKFFTDIRNDIKPRFLPII++KIA YDTF++HDTHDLP+VRAAEDAVQREL ++V
Sbjct: 181 YKNNLKKFFTDIRNDIKPRFLPIIVIKIAGYDTFIEHDTHDLPTVRAAEDAVQRELLNIV 240

Query: 241 TIDSLKLVNTRTHEGFNQDHGHFNIKTEIALGKWLADTYLSHYGHLL 288
           TID+LKLVNT T EGFN D GH+N+KTEIALGKWLADTYLSHYG LL
Sbjct: 241 TIDALKLVNTITGEGFNLDRGHYNVKTEIALGKWLADTYLSHYGQLL 287

BLAST of Spg025305 vs. NCBI nr
Match: XP_022157447.1 (probable carbohydrate esterase At4g34215 [Momordica charantia])

HSP 1 Score: 498.4 bits (1282), Expect = 4.0e-137
Identity = 245/287 (85.37%), Postives = 264/287 (91.99%), Query Frame = 0

Query: 1   MALLKLSILLCTMLFGLSLSGATSPQNIFILAGQSNMAGRGGVEKDHSGVLVWDGYVPPE 60
           M +L+LSILLC ML G SLS ATSP+NIFILAGQSNMAGRGGVE D  G LVWDGYVPPE
Sbjct: 1   MVILRLSILLCMMLCGPSLSRATSPKNIFILAGQSNMAGRGGVENDRPGHLVWDGYVPPE 60

Query: 61  AQPDPSILRLSPERQWEIALEPVHEGIDINKTVGVGSAMAFAHHLQAKAGAKAGVVGLVP 120
           AQPDPSILRL+PERQWE+A EPVHEGIDINKTVGVG A+AFA  LQAK G+K G VGLVP
Sbjct: 61  AQPDPSILRLNPERQWEVAREPVHEGIDINKTVGVGPAIAFARQLQAKGGSKVGSVGLVP 120

Query: 121 CARGGTLIEQWIKNPNNPNATFYQNFIERIKASDKKGGVVRALFWLQGESDAAASDTASR 180
           CARGGTLIEQW+KNP+N +ATFY+NFIERI+ASD++GGVVRALFWLQGESDAA+ DTA+R
Sbjct: 121 CARGGTLIEQWVKNPSNTSATFYKNFIERIQASDREGGVVRALFWLQGESDAASRDTANR 180

Query: 181 YKNNLKKFFTDIRNDIKPRFLPIILVKIAVYDTFLKHDTHDLPSVRAAEDAVQRELPDVV 240
           YKNNLKKFFTDIRNDIKPRFLPIILVKIAVYDTF+KHDTHDLP+VRAAEDAVQRELP+VV
Sbjct: 181 YKNNLKKFFTDIRNDIKPRFLPIILVKIAVYDTFMKHDTHDLPAVRAAEDAVQRELPNVV 240

Query: 241 TIDSLKLVNTRTHEGFNQDHGHFNIKTEIALGKWLADTYLSHYGHLL 288
           TIDSLKLVNT T EGFN D GHFNIKTEIALGKWLADTYLS YGHLL
Sbjct: 241 TIDSLKLVNTTTVEGFNLDRGHFNIKTEIALGKWLADTYLSQYGHLL 287

BLAST of Spg025305 vs. NCBI nr
Match: XP_023002178.1 (probable carbohydrate esterase At4g34215 [Cucurbita maxima])

HSP 1 Score: 497.3 bits (1279), Expect = 9.0e-137
Identity = 242/287 (84.32%), Postives = 263/287 (91.64%), Query Frame = 0

Query: 1   MALLKLSILLCTMLFGLSLSGATSPQNIFILAGQSNMAGRGGVEKDHSGVLVWDGYVPPE 60
           MA L+L+I +C MLF  SL GATSP+NIFILAGQSNMAGRGGVEKDH G LVWDGY+PPE
Sbjct: 1   MAWLRLAIWVCMMLFYPSLLGATSPKNIFILAGQSNMAGRGGVEKDHMGQLVWDGYIPPE 60

Query: 61  AQPDPSILRLSPERQWEIALEPVHEGIDINKTVGVGSAMAFAHHLQAKAGAKAGVVGLVP 120
           A  DP+ILR SPE QWEIALEPVH+GIDINKTVGVGSA+ FA  LQAK+ ++AGVVGLVP
Sbjct: 61  AYSDPTILRFSPEGQWEIALEPVHDGIDINKTVGVGSAIVFARQLQAKSKSEAGVVGLVP 120

Query: 121 CARGGTLIEQWIKNPNNPNATFYQNFIERIKASDKKGGVVRALFWLQGESDAAASDTASR 180
           CARGGTLIE+WIKNP+NP ATFYQNFIERIKAS+K GGVVRAL WLQGESDAAASDTA R
Sbjct: 121 CARGGTLIEEWIKNPSNPEATFYQNFIERIKASEKDGGVVRALIWLQGESDAAASDTAHR 180

Query: 181 YKNNLKKFFTDIRNDIKPRFLPIILVKIAVYDTFLKHDTHDLPSVRAAEDAVQRELPDVV 240
           YK+NLKKFF DIRNDIKPRFLPIILVKIAVYDT++KHDTHDLP+VRAAEDAVQ+ELPD+V
Sbjct: 181 YKDNLKKFFMDIRNDIKPRFLPIILVKIAVYDTYMKHDTHDLPAVRAAEDAVQKELPDIV 240

Query: 241 TIDSLKLVNTRTHEGFNQDHGHFNIKTEIALGKWLADTYLSHYGHLL 288
           TIDSL LVNT THEGFNQDHGHFN KT+IALGKWLADTYLSHYGHLL
Sbjct: 241 TIDSLNLVNTVTHEGFNQDHGHFNTKTQIALGKWLADTYLSHYGHLL 287

BLAST of Spg025305 vs. NCBI nr
Match: XP_022158593.1 (probable carbohydrate esterase At4g34215 [Momordica charantia])

HSP 1 Score: 488.0 bits (1255), Expect = 5.5e-134
Identity = 235/275 (85.45%), Postives = 257/275 (93.45%), Query Frame = 0

Query: 13  MLFGLSLSGATSPQNIFILAGQSNMAGRGGVEKDHSGVLVWDGYVPPEAQPDPSILRLSP 72
           +L G SLSGATSP+NIFILAGQSNMAGRGGVEKD SG LVWDGYVPPEAQPDPSILRL+P
Sbjct: 2   ILCGPSLSGATSPKNIFILAGQSNMAGRGGVEKDRSGNLVWDGYVPPEAQPDPSILRLNP 61

Query: 73  ERQWEIALEPVHEGIDINKTVGVGSAMAFAHHLQAKAGAKAGVVGLVPCARGGTLIEQWI 132
           ERQWE+A EPVHEGIDINKTVGVGSA+AFAH LQAK G+K G VGLVPCARGGTLIEQW+
Sbjct: 62  ERQWEVAREPVHEGIDINKTVGVGSAIAFAHQLQAKGGSKVGSVGLVPCARGGTLIEQWV 121

Query: 133 KNPNNPNATFYQNFIERIKASDKKGGVVRALFWLQGESDAAASDTASRYKNNLKKFFTDI 192
           KNP+N +ATFY+NFIERI+ASD++GGVVRALFWLQGESDAA+ DTASRYKNNLKKFFTDI
Sbjct: 122 KNPSNTSATFYKNFIERIQASDREGGVVRALFWLQGESDAASRDTASRYKNNLKKFFTDI 181

Query: 193 RNDIKPRFLPIILVKIAVYDTFLKHDTHDLPSVRAAEDAVQRELPDVVTIDSLKLVNTRT 252
           RNDIKPRFLPII+VKIA YDTF++HDTHDLP+VRAAEDAVQRELP++VTID+LKLVNT T
Sbjct: 182 RNDIKPRFLPIIVVKIAGYDTFMEHDTHDLPAVRAAEDAVQRELPNIVTIDALKLVNTVT 241

Query: 253 HEGFNQDHGHFNIKTEIALGKWLADTYLSHYGHLL 288
            EGFN D GH+N KTEIALGKWLADTYLSHYG LL
Sbjct: 242 GEGFNSDRGHYNFKTEIALGKWLADTYLSHYGQLL 276

BLAST of Spg025305 vs. ExPASy Swiss-Prot
Match: Q8L9J9 (Probable carbohydrate esterase At4g34215 OS=Arabidopsis thaliana OX=3702 GN=At4g34215 PE=1 SV=2)

HSP 1 Score: 177.6 bits (449), Expect = 2.1e-43
Identity = 101/260 (38.85%), Postives = 145/260 (55.77%), Query Frame = 0

Query: 25  PQNIFILAGQSNMAGRGGVEKD-HSGVLVWDGYVPPEAQPDPSILRLSPERQWEIALEPV 84
           P  IFIL+GQSNMAGRGGV KD H+   VWD  +PPE  P+ SILRLS + +WE A EP+
Sbjct: 21  PNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEEAHEPL 80

Query: 85  HEGIDINKTVGVGSAMAFAHHLQAKAGAKAGVVGLVPCARGGTLIEQWIKNPNNPNATFY 144
           H  ID  K  GVG  MAFA+ ++ +    + V+GLVPCA GGT I++W +  +      Y
Sbjct: 81  HVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWERGSH-----LY 140

Query: 145 QNFIERIKASDKKGGVVRALFWLQGESDAAASDTASRYKNNLKKFFTDIRNDIKPRFLPI 204
           +  ++R + S K GG ++A+ W QGESD      A  Y NN+ +   ++R+D+    LPI
Sbjct: 141 ERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLPI 200

Query: 205 ILVKIAVYDTFLKHDTHDLPSVRAAEDAVQRELPDVVTIDSLKLVNTRTHEGFNQDHGHF 264
           I V IA    ++          +  E  +  +L +VV +D+  L           D+ H 
Sbjct: 201 IQVAIASGGGYID---------KVREAQLGLKLSNVVCVDAKGL-------PLKSDNLHL 259

Query: 265 NIKTEIALGKWLADTYLSHY 284
             + ++ LG  LA  YLS++
Sbjct: 261 TTEAQVQLGLSLAQAYLSNF 259

BLAST of Spg025305 vs. ExPASy TrEMBL
Match: A0A6J1BQ38 (probable carbohydrate esterase At4g34215 OS=Momordica charantia OX=3673 GN=LOC111004778 PE=4 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 1.2e-139
Identity = 246/287 (85.71%), Postives = 268/287 (93.38%), Query Frame = 0

Query: 1   MALLKLSILLCTMLFGLSLSGATSPQNIFILAGQSNMAGRGGVEKDHSGVLVWDGYVPPE 60
           MALL+LSI+LC MLFG SLSGATSP+NIFILAGQSNMAGRGGVEK+ +G L WDGYVPPE
Sbjct: 1   MALLRLSIMLCMMLFGPSLSGATSPKNIFILAGQSNMAGRGGVEKNRTGDLEWDGYVPPE 60

Query: 61  AQPDPSILRLSPERQWEIALEPVHEGIDINKTVGVGSAMAFAHHLQAKAGAKAGVVGLVP 120
           +QPDPSILRL+PERQWE+A EPVH GIDI KTVGVG A+AFAH LQAK G+K G VGLVP
Sbjct: 61  SQPDPSILRLNPERQWEVAREPVHRGIDIGKTVGVGPAIAFAHQLQAKGGSKVGSVGLVP 120

Query: 121 CARGGTLIEQWIKNPNNPNATFYQNFIERIKASDKKGGVVRALFWLQGESDAAASDTASR 180
           CARGGTLIEQW+KNP+NPNATFY+NFIERI+ASD++GGVVRALFWLQGESDAA+SDTA R
Sbjct: 121 CARGGTLIEQWVKNPSNPNATFYKNFIERIQASDREGGVVRALFWLQGESDAASSDTAER 180

Query: 181 YKNNLKKFFTDIRNDIKPRFLPIILVKIAVYDTFLKHDTHDLPSVRAAEDAVQRELPDVV 240
           YKNNLKKFFTDIRNDIKPR LPIILVKIAVYDTF+KHDTHDLP+VRAAEDAVQRELP+VV
Sbjct: 181 YKNNLKKFFTDIRNDIKPRVLPIILVKIAVYDTFMKHDTHDLPAVRAAEDAVQRELPNVV 240

Query: 241 TIDSLKLVNTRTHEGFNQDHGHFNIKTEIALGKWLADTYLSHYGHLL 288
           TID+LKLVNT T EGFN D GHFNIKTEIALGKWLADTYLS+YGHLL
Sbjct: 241 TIDALKLVNTTTAEGFNLDRGHFNIKTEIALGKWLADTYLSNYGHLL 287

BLAST of Spg025305 vs. ExPASy TrEMBL
Match: A0A6J1DW87 (probable carbohydrate esterase At4g34215 OS=Momordica charantia OX=3673 GN=LOC111025037 PE=4 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 1.1e-137
Identity = 243/287 (84.67%), Postives = 268/287 (93.38%), Query Frame = 0

Query: 1   MALLKLSILLCTMLFGLSLSGATSPQNIFILAGQSNMAGRGGVEKDHSGVLVWDGYVPPE 60
           MALL+LSILLC +L G SLSGATSP+NIFILAGQSNMAGRGGVEKD SG LVWDGYVPPE
Sbjct: 1   MALLRLSILLCMILCGPSLSGATSPKNIFILAGQSNMAGRGGVEKDRSGNLVWDGYVPPE 60

Query: 61  AQPDPSILRLSPERQWEIALEPVHEGIDINKTVGVGSAMAFAHHLQAKAGAKAGVVGLVP 120
           AQPDPSILRL+PERQWE+A EPVHEGIDINKTVGVGSA+AFAH LQAK G+K G VGLVP
Sbjct: 61  AQPDPSILRLNPERQWEVAREPVHEGIDINKTVGVGSAIAFAHQLQAKGGSKVGSVGLVP 120

Query: 121 CARGGTLIEQWIKNPNNPNATFYQNFIERIKASDKKGGVVRALFWLQGESDAAASDTASR 180
           CARGGTLIEQW+KNP+N +ATFY+NFIERI+ASD++GGVVRALFWLQGESDAA+ DTASR
Sbjct: 121 CARGGTLIEQWVKNPSNTSATFYKNFIERIQASDREGGVVRALFWLQGESDAASRDTASR 180

Query: 181 YKNNLKKFFTDIRNDIKPRFLPIILVKIAVYDTFLKHDTHDLPSVRAAEDAVQRELPDVV 240
           YKNNLKKFFTDIRNDIKPRFLPII++KIA YDTF++HDTHDLP+VRAAEDAVQREL ++V
Sbjct: 181 YKNNLKKFFTDIRNDIKPRFLPIIVIKIAGYDTFIEHDTHDLPTVRAAEDAVQRELLNIV 240

Query: 241 TIDSLKLVNTRTHEGFNQDHGHFNIKTEIALGKWLADTYLSHYGHLL 288
           TID+LKLVNT T EGFN D GH+N+KTEIALGKWLADTYLSHYG LL
Sbjct: 241 TIDALKLVNTITGEGFNLDRGHYNVKTEIALGKWLADTYLSHYGQLL 287

BLAST of Spg025305 vs. ExPASy TrEMBL
Match: A0A6J1DUH7 (probable carbohydrate esterase At4g34215 OS=Momordica charantia OX=3673 GN=LOC111024143 PE=4 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 2.0e-137
Identity = 245/287 (85.37%), Postives = 264/287 (91.99%), Query Frame = 0

Query: 1   MALLKLSILLCTMLFGLSLSGATSPQNIFILAGQSNMAGRGGVEKDHSGVLVWDGYVPPE 60
           M +L+LSILLC ML G SLS ATSP+NIFILAGQSNMAGRGGVE D  G LVWDGYVPPE
Sbjct: 1   MVILRLSILLCMMLCGPSLSRATSPKNIFILAGQSNMAGRGGVENDRPGHLVWDGYVPPE 60

Query: 61  AQPDPSILRLSPERQWEIALEPVHEGIDINKTVGVGSAMAFAHHLQAKAGAKAGVVGLVP 120
           AQPDPSILRL+PERQWE+A EPVHEGIDINKTVGVG A+AFA  LQAK G+K G VGLVP
Sbjct: 61  AQPDPSILRLNPERQWEVAREPVHEGIDINKTVGVGPAIAFARQLQAKGGSKVGSVGLVP 120

Query: 121 CARGGTLIEQWIKNPNNPNATFYQNFIERIKASDKKGGVVRALFWLQGESDAAASDTASR 180
           CARGGTLIEQW+KNP+N +ATFY+NFIERI+ASD++GGVVRALFWLQGESDAA+ DTA+R
Sbjct: 121 CARGGTLIEQWVKNPSNTSATFYKNFIERIQASDREGGVVRALFWLQGESDAASRDTANR 180

Query: 181 YKNNLKKFFTDIRNDIKPRFLPIILVKIAVYDTFLKHDTHDLPSVRAAEDAVQRELPDVV 240
           YKNNLKKFFTDIRNDIKPRFLPIILVKIAVYDTF+KHDTHDLP+VRAAEDAVQRELP+VV
Sbjct: 181 YKNNLKKFFTDIRNDIKPRFLPIILVKIAVYDTFMKHDTHDLPAVRAAEDAVQRELPNVV 240

Query: 241 TIDSLKLVNTRTHEGFNQDHGHFNIKTEIALGKWLADTYLSHYGHLL 288
           TIDSLKLVNT T EGFN D GHFNIKTEIALGKWLADTYLS YGHLL
Sbjct: 241 TIDSLKLVNTTTVEGFNLDRGHFNIKTEIALGKWLADTYLSQYGHLL 287

BLAST of Spg025305 vs. ExPASy TrEMBL
Match: A0A6J1KST3 (probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC111496117 PE=4 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 4.4e-137
Identity = 242/287 (84.32%), Postives = 263/287 (91.64%), Query Frame = 0

Query: 1   MALLKLSILLCTMLFGLSLSGATSPQNIFILAGQSNMAGRGGVEKDHSGVLVWDGYVPPE 60
           MA L+L+I +C MLF  SL GATSP+NIFILAGQSNMAGRGGVEKDH G LVWDGY+PPE
Sbjct: 1   MAWLRLAIWVCMMLFYPSLLGATSPKNIFILAGQSNMAGRGGVEKDHMGQLVWDGYIPPE 60

Query: 61  AQPDPSILRLSPERQWEIALEPVHEGIDINKTVGVGSAMAFAHHLQAKAGAKAGVVGLVP 120
           A  DP+ILR SPE QWEIALEPVH+GIDINKTVGVGSA+ FA  LQAK+ ++AGVVGLVP
Sbjct: 61  AYSDPTILRFSPEGQWEIALEPVHDGIDINKTVGVGSAIVFARQLQAKSKSEAGVVGLVP 120

Query: 121 CARGGTLIEQWIKNPNNPNATFYQNFIERIKASDKKGGVVRALFWLQGESDAAASDTASR 180
           CARGGTLIE+WIKNP+NP ATFYQNFIERIKAS+K GGVVRAL WLQGESDAAASDTA R
Sbjct: 121 CARGGTLIEEWIKNPSNPEATFYQNFIERIKASEKDGGVVRALIWLQGESDAAASDTAHR 180

Query: 181 YKNNLKKFFTDIRNDIKPRFLPIILVKIAVYDTFLKHDTHDLPSVRAAEDAVQRELPDVV 240
           YK+NLKKFF DIRNDIKPRFLPIILVKIAVYDT++KHDTHDLP+VRAAEDAVQ+ELPD+V
Sbjct: 181 YKDNLKKFFMDIRNDIKPRFLPIILVKIAVYDTYMKHDTHDLPAVRAAEDAVQKELPDIV 240

Query: 241 TIDSLKLVNTRTHEGFNQDHGHFNIKTEIALGKWLADTYLSHYGHLL 288
           TIDSL LVNT THEGFNQDHGHFN KT+IALGKWLADTYLSHYGHLL
Sbjct: 241 TIDSLNLVNTVTHEGFNQDHGHFNTKTQIALGKWLADTYLSHYGHLL 287

BLAST of Spg025305 vs. ExPASy TrEMBL
Match: A0A6J1DWJ1 (probable carbohydrate esterase At4g34215 OS=Momordica charantia OX=3673 GN=LOC111025047 PE=4 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 2.6e-134
Identity = 235/275 (85.45%), Postives = 257/275 (93.45%), Query Frame = 0

Query: 13  MLFGLSLSGATSPQNIFILAGQSNMAGRGGVEKDHSGVLVWDGYVPPEAQPDPSILRLSP 72
           +L G SLSGATSP+NIFILAGQSNMAGRGGVEKD SG LVWDGYVPPEAQPDPSILRL+P
Sbjct: 2   ILCGPSLSGATSPKNIFILAGQSNMAGRGGVEKDRSGNLVWDGYVPPEAQPDPSILRLNP 61

Query: 73  ERQWEIALEPVHEGIDINKTVGVGSAMAFAHHLQAKAGAKAGVVGLVPCARGGTLIEQWI 132
           ERQWE+A EPVHEGIDINKTVGVGSA+AFAH LQAK G+K G VGLVPCARGGTLIEQW+
Sbjct: 62  ERQWEVAREPVHEGIDINKTVGVGSAIAFAHQLQAKGGSKVGSVGLVPCARGGTLIEQWV 121

Query: 133 KNPNNPNATFYQNFIERIKASDKKGGVVRALFWLQGESDAAASDTASRYKNNLKKFFTDI 192
           KNP+N +ATFY+NFIERI+ASD++GGVVRALFWLQGESDAA+ DTASRYKNNLKKFFTDI
Sbjct: 122 KNPSNTSATFYKNFIERIQASDREGGVVRALFWLQGESDAASRDTASRYKNNLKKFFTDI 181

Query: 193 RNDIKPRFLPIILVKIAVYDTFLKHDTHDLPSVRAAEDAVQRELPDVVTIDSLKLVNTRT 252
           RNDIKPRFLPII+VKIA YDTF++HDTHDLP+VRAAEDAVQRELP++VTID+LKLVNT T
Sbjct: 182 RNDIKPRFLPIIVVKIAGYDTFMEHDTHDLPAVRAAEDAVQRELPNIVTIDALKLVNTVT 241

Query: 253 HEGFNQDHGHFNIKTEIALGKWLADTYLSHYGHLL 288
            EGFN D GH+N KTEIALGKWLADTYLSHYG LL
Sbjct: 242 GEGFNSDRGHYNFKTEIALGKWLADTYLSHYGQLL 276

BLAST of Spg025305 vs. TAIR 10
Match: AT4G34215.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 177.6 bits (449), Expect = 1.5e-44
Identity = 101/260 (38.85%), Postives = 145/260 (55.77%), Query Frame = 0

Query: 25  PQNIFILAGQSNMAGRGGVEKD-HSGVLVWDGYVPPEAQPDPSILRLSPERQWEIALEPV 84
           P  IFIL+GQSNMAGRGGV KD H+   VWD  +PPE  P+ SILRLS + +WE A EP+
Sbjct: 21  PNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEEAHEPL 80

Query: 85  HEGIDINKTVGVGSAMAFAHHLQAKAGAKAGVVGLVPCARGGTLIEQWIKNPNNPNATFY 144
           H  ID  K  GVG  MAFA+ ++ +    + V+GLVPCA GGT I++W +  +      Y
Sbjct: 81  HVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWERGSH-----LY 140

Query: 145 QNFIERIKASDKKGGVVRALFWLQGESDAAASDTASRYKNNLKKFFTDIRNDIKPRFLPI 204
           +  ++R + S K GG ++A+ W QGESD      A  Y NN+ +   ++R+D+    LPI
Sbjct: 141 ERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLPI 200

Query: 205 ILVKIAVYDTFLKHDTHDLPSVRAAEDAVQRELPDVVTIDSLKLVNTRTHEGFNQDHGHF 264
           I V IA    ++          +  E  +  +L +VV +D+  L           D+ H 
Sbjct: 201 IQVAIASGGGYID---------KVREAQLGLKLSNVVCVDAKGL-------PLKSDNLHL 259

Query: 265 NIKTEIALGKWLADTYLSHY 284
             + ++ LG  LA  YLS++
Sbjct: 261 TTEAQVQLGLSLAQAYLSNF 259

BLAST of Spg025305 vs. TAIR 10
Match: AT4G34215.2 (Domain of unknown function (DUF303) )

HSP 1 Score: 177.6 bits (449), Expect = 1.5e-44
Identity = 101/260 (38.85%), Postives = 145/260 (55.77%), Query Frame = 0

Query: 25  PQNIFILAGQSNMAGRGGVEKD-HSGVLVWDGYVPPEAQPDPSILRLSPERQWEIALEPV 84
           P  IFIL+GQSNMAGRGGV KD H+   VWD  +PPE  P+ SILRLS + +WE A EP+
Sbjct: 21  PNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEEAHEPL 80

Query: 85  HEGIDINKTVGVGSAMAFAHHLQAKAGAKAGVVGLVPCARGGTLIEQWIKNPNNPNATFY 144
           H  ID  K  GVG  MAFA+ ++ +    + V+GLVPCA GGT I++W +  +      Y
Sbjct: 81  HVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWERGSH-----LY 140

Query: 145 QNFIERIKASDKKGGVVRALFWLQGESDAAASDTASRYKNNLKKFFTDIRNDIKPRFLPI 204
           +  ++R + S K GG ++A+ W QGESD      A  Y NN+ +   ++R+D+    LPI
Sbjct: 141 ERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLPI 200

Query: 205 ILVKIAVYDTFLKHDTHDLPSVRAAEDAVQRELPDVVTIDSLKLVNTRTHEGFNQDHGHF 264
           I V IA    ++          +  E  +  +L +VV +D+  L           D+ H 
Sbjct: 201 IQVAIASGGGYID---------KVREAQLGLKLSNVVCVDAKGL-------PLKSDNLHL 259

Query: 265 NIKTEIALGKWLADTYLSHY 284
             + ++ LG  LA  YLS++
Sbjct: 261 TTEAQVQLGLSLAQAYLSNF 259

BLAST of Spg025305 vs. TAIR 10
Match: AT3G53010.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 170.2 bits (430), Expect = 2.3e-42
Identity = 106/258 (41.09%), Postives = 147/258 (56.98%), Query Frame = 0

Query: 27  NIFILAGQSNMAGRGGVEKD-HSGVLVWDGYVPPEAQPDPSILRLSPERQWEIALEPVHE 86
           +IFILAGQSNMAGRGGV  D  +   VWDG +PPE + +PSILRL+ + +W+ A EP+H 
Sbjct: 30  SIFILAGQSNMAGRGGVYNDTATNTTVWDGVIPPECRSNPSILRLTSKLEWKEAKEPLHV 89

Query: 87  GIDINKTVGVGSAMAFAHHLQAKAGAKAGVVGLVPCARGGTLIEQWIKNPNNPNATFYQN 146
            IDINKT GVG  M FA+ +  + G     VGLVPC+ GGT + QW K         Y+ 
Sbjct: 90  DIDINKTNGVGPGMPFANRVVNRFGQ----VGLVPCSIGGTKLSQWQK-----GEFLYEE 149

Query: 147 FIERIKA--SDKKGGVVRALFWLQGESDAAASDTASRYKNNLKKFFTDIRNDIKPRFLPI 206
            ++R KA  +   GG  RA+ W QGESD      AS YK  L KFF+D+RND++   LPI
Sbjct: 150 TVKRAKAAMASGGGGSYRAVLWYQGESDTVDMVDASVYKKRLVKFFSDLRNDLQHPNLPI 209

Query: 207 ILVKIAVYDTFLKHDTHDLPSVRAAEDAVQRELPDVVTIDSLKLVNTRTHEGFNQDHGHF 266
           I V +A            L +VR A+  ++ +L +V  +D+  L           D  H 
Sbjct: 210 IQVALAT------GAGPYLDAVRKAQ--LKTDLENVYCVDARGL-------PLEPDGLHL 263

Query: 267 NIKTEIALGKWLADTYLS 282
              +++ LG  +A+++L+
Sbjct: 270 TTSSQVQLGHMIAESFLA 263