Homology
BLAST of Spg025294 vs. NCBI nr
Match:
XP_023546329.1 (subtilisin-like protease SBT1.9 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1113.6 bits (2879), Expect = 0.0e+00
Identity = 583/768 (75.91%), Postives = 631/768 (82.16%), Query Frame = 0
Query: 1 MAAHMCMYFWFSLMAIFWLPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSV-LDN 60
MAAHM M+FW SLM IF T TET+NYIVHMDLAAMPKPFA+HHSWYSATLSSV LD
Sbjct: 1 MAAHMRMFFWISLMVIFLARSTVTETDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDT 60
Query: 61 SRLGTMASSSSSSLSSKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDT 120
S L T +SSSSSL SKLIH YKHAINGF+A+LTPS+L+AL+ SPGYVSSVRDSSV+ DT
Sbjct: 61 SPLRT-TTSSSSSLPSKLIHCYKHAINGFTANLTPSQLDALKNSPGYVSSVRDSSVRADT 120
Query: 121 THSSHFLGLSSNSGLLPISNYGSDVIIGVVDTGVWPESGSFNDDGMFEIPSRWKGECESG 180
THSS+FL LS NSGLLPISNYGSDVIIG VDTGVWPES SFNDDG+ +IPSRWKGECESG
Sbjct: 121 THSSNFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESG 180
Query: 181 AHFSGSLCNKKLIGARFFNKGLIAKFPNITISMNSTRDTNGHGTHTSTTAAGSYVKEASF 240
HF+ SLCNKKLIG RFFNKGLIAKFPN+TISMNSTRDTNGHGTHTSTTAAGSYVKEASF
Sbjct: 181 THFNASLCNKKLIGGRFFNKGLIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASF 240
Query: 241 FGYGLGIARGVAPRARVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVDGVPLY 300
FGYG G ARGVAPRARVAIYKA+W EGN VSDVIAAID AI DGVDVI+LSLG+D VPLY
Sbjct: 241 FGYGQGTARGVAPRARVAIYKAIWKEGNVVSDVIAAIDQAISDGVDVIALSLGIDRVPLY 300
Query: 301 EDPIAIATYAAMERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFG-------- 360
EDP+AIAT+AAMERGIFVATSAGN GPQ GTVH+GAPWVLNVAAGTMDRDFG
Sbjct: 301 EDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNG 360
Query: 361 ------------------------------------------------------------ 420
Sbjct: 361 VSVLGSSLFPLNSAIALSPLPIFFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVQ 420
Query: 421 --EVAVGVFISNISDWDNLIQTPFPSIFLSPYHGNIIKDYIKGNSDPKARVNFHKTILGT 480
++AVGVFISNISDWDNLIQT FPSIFL+ YHGN++KDYI +S+PKARVNFHKTI+GT
Sbjct: 421 SAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVLKDYINRSSNPKARVNFHKTIIGT 480
Query: 481 KPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSG 540
KPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDV SRPIYSKFNVLSG
Sbjct: 481 KPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNVLSG 540
Query: 541 TSMSCPHAAGVAALLKGAHPRWSLAAIRSAMMTTADVLDNTQTPIKDLGDRNKLATPLAM 600
TSM+CPHAAGVAALLKGAHP WS AAIRSAMMTTAD++DNTQT IKDLG++NK+ATPLAM
Sbjct: 541 TSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAM 600
Query: 601 GSGHVNPNKAMDPGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLDLNYPS 660
GSGHVNPNKA+DPGL+YDMGI+DY NL+CALNYTKNQIQTITRSTSN+CE PSLDLNYPS
Sbjct: 601 GSGHVNPNKAIDPGLIYDMGIEDYTNLICALNYTKNQIQTITRSTSNDCEKPSLDLNYPS 660
Query: 661 FIIIVNASDSKATR-EISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKN 694
FII VNASDS+ R EISREF R VTNIGEKG TY+AK+ M GL VTV+PKKLKFK KN
Sbjct: 661 FIITVNASDSETGRTEISREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPKKLKFKRKN 720
BLAST of Spg025294 vs. NCBI nr
Match:
XP_022962414.1 (subtilisin-like protease SBT1.9 [Cucurbita moschata])
HSP 1 Score: 1110.9 bits (2872), Expect = 0.0e+00
Identity = 583/775 (75.23%), Postives = 634/775 (81.81%), Query Frame = 0
Query: 1 MAAHMCMYFWFSLMAIFWLPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSV-LDN 60
MAAHM M+FW SLM IF T T+T+NYIVHMDLAAMPKPFA+HHSWYSATLSSV LD
Sbjct: 1 MAAHMRMFFWVSLMVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDT 60
Query: 61 SRLGTMASSSSSSLSSKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDT 120
S L T SSSSSSL SKLIH YKHAINGF+ASLTPS+L+AL+ SPGYVSS+RDSSV+ DT
Sbjct: 61 SPLRTTTSSSSSSLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSSVRADT 120
Query: 121 THSSHFLGLSSNSGLLPISNYGSDVIIGVVDTGVWPESGSFNDDGMFEIPSRWKGECESG 180
THSS+FL LS NSGLLPISNYGSDVIIG VDTGVWPES SFNDDG+ +IPSRWKGECESG
Sbjct: 121 THSSNFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESG 180
Query: 181 AHFSGSLCNKKLIGARFFNKGLIAKFPNITISMNSTRDTNGHGTHTSTTAAGSYVKEASF 240
HF+ SLCNKKLIG RFFNKGLI+KFPN+TISMNSTRDTNGHGTHTSTTAAGSYVKEASF
Sbjct: 181 THFNASLCNKKLIGGRFFNKGLISKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASF 240
Query: 241 FGYGLGIARGVAPRARVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVDGVPLY 300
FGYG G ARGVAPRARVAIYKA+W EGN VSDVIAAID AI DGVDVISLSLG+DGVPLY
Sbjct: 241 FGYGQGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLY 300
Query: 301 EDPIAIATYAAMERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFG-------- 360
EDP+AIAT+AAMERGIFVATSAGN GPQ GTVH+GAPWVLNVAAGTMDRDFG
Sbjct: 301 EDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNG 360
Query: 361 ------------------------------------------------------------ 420
Sbjct: 361 VSVLGSSLFPLNSAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVE 420
Query: 421 --EVAVGVFISNISDWDNLIQTPFPSIFLSPYHGNIIKDYIKGNSDPKARVNFHKTILGT 480
++AVGVFISNISDWDNLIQT FPSIFL+ YHGN+IKDYIK +S+PKARVNFHKTI+GT
Sbjct: 421 SAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGT 480
Query: 481 KPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSG 540
KPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDV SRPIYSKFNVLSG
Sbjct: 481 KPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNVLSG 540
Query: 541 TSMSCPHAAGVAALLKGAHPRWSLAAIRSAMMTTADVLDNTQTPIKDLGDRNKLATPLAM 600
TSM+CPHAAGVAALLKGAHP WS AAIRSAMMTTAD++DNTQT IKDLG++NK+ATPLAM
Sbjct: 541 TSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAM 600
Query: 601 GSGHVNPNKAMDPGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLDLNYPS 660
GSGHVNPNKA+DPGL+YDMGI+DY NLLCALNYTKNQIQTITRSTSN+CE LDLNYPS
Sbjct: 601 GSGHVNPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKALLDLNYPS 660
Query: 661 FIIIVNASDSKATR-EISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKN 701
FI+ VNASDS+ R E+SREF R VTNIGEKG TY+AK+ M GL VTV+P KLKFK KN
Sbjct: 661 FIMTVNASDSQTGRTEMSREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKN 720
BLAST of Spg025294 vs. NCBI nr
Match:
KAG6598336.1 (Subtilisin-like protease 3, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1107.4 bits (2863), Expect = 0.0e+00
Identity = 584/775 (75.35%), Postives = 634/775 (81.81%), Query Frame = 0
Query: 1 MAAHMCMYFWFSLMAIFWLPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSV-LDN 60
MAAHM M+FW SL+ IF T T+T+NYIVHMDLAAMPKPFA+HHSWYSATLSSV LD
Sbjct: 1 MAAHMRMFFWVSLIVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDT 60
Query: 61 SRLGTMASSSSSSLSSKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDT 120
S L T SSSSSS SKLIH YKHAINGF+A+LTPS+L+AL+ SPGYVSSVRDSSV+ DT
Sbjct: 61 SPLRTTTSSSSSS-PSKLIHCYKHAINGFTANLTPSQLDALKNSPGYVSSVRDSSVRADT 120
Query: 121 THSSHFLGLSSNSGLLPISNYGSDVIIGVVDTGVWPESGSFNDDGMFEIPSRWKGECESG 180
THSS+FL LS NSGLLPISNYGSDVIIG VDTGVWPES SFNDDG+ +IPSRWKGECESG
Sbjct: 121 THSSNFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGIPKIPSRWKGECESG 180
Query: 181 AHFSGSLCNKKLIGARFFNKGLIAKFPNITISMNSTRDTNGHGTHTSTTAAGSYVKEASF 240
HF+ SLCNKKLIG RFFNKGLIAKFPN+TISMNSTRDTNGHGTHTSTTAAGSYVKEASF
Sbjct: 181 THFNASLCNKKLIGGRFFNKGLIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASF 240
Query: 241 FGYGLGIARGVAPRARVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVDGVPLY 300
FGYG G ARGVAPRARVAIYKA+W EGN VSDVIAAID AI DGVDVISLSLG+DGVPLY
Sbjct: 241 FGYGQGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLY 300
Query: 301 EDPIAIATYAAMERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFG-------- 360
EDP+AIAT+AAMERGIFVATSAGN GPQ GTVH+GAPWVLNVAAGTMDRDFG
Sbjct: 301 EDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNG 360
Query: 361 ------------------------------------------------------------ 420
Sbjct: 361 VSVLGSSLFPLNLAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVE 420
Query: 421 --EVAVGVFISNISDWDNLIQTPFPSIFLSPYHGNIIKDYIKGNSDPKARVNFHKTILGT 480
++AVGVFISNISDWDNLIQT FPSIFL+ YHGN+IKDYIK +S+PKARVNFHKTI+GT
Sbjct: 421 SAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGT 480
Query: 481 KPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSG 540
KPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDV SRPIYSK+NVLSG
Sbjct: 481 KPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKYNVLSG 540
Query: 541 TSMSCPHAAGVAALLKGAHPRWSLAAIRSAMMTTADVLDNTQTPIKDLGDRNKLATPLAM 600
TSM+CPHAAGVAALLKGAHP WS AAIRSAMMTTAD++DNTQT IKDLG++NK+ATPLAM
Sbjct: 541 TSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAM 600
Query: 601 GSGHVNPNKAMDPGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLDLNYPS 660
GSGHVNPNKA+DPGL+YDMGI+DY NLLCALNYTKNQIQTITRSTSN+CE P LDLNYPS
Sbjct: 601 GSGHVNPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKPLLDLNYPS 660
Query: 661 FIIIVNASDSKATR-EISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKN 701
FII VNASDS+ R EISREF R VTNIGEKG TY+AK+ M GL VTV+P KLKFK KN
Sbjct: 661 FIITVNASDSQTGRTEISREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKN 720
BLAST of Spg025294 vs. NCBI nr
Match:
KAG7029306.1 (Subtilisin-like protease SBT1.9, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1104.7 bits (2856), Expect = 0.0e+00
Identity = 583/775 (75.23%), Postives = 632/775 (81.55%), Query Frame = 0
Query: 1 MAAHMCMYFWFSLMAIFWLPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSV-LDN 60
MAAHM M+FW SLM IF T T+T+NYIVHMDLAAMP FA+HHSWYSATLSSV LD
Sbjct: 1 MAAHMRMFFWVSLMVIFLARSTLTQTDNYIVHMDLAAMPNSFATHHSWYSATLSSVLLDT 60
Query: 61 SRLGTMASSSSSSLSSKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDT 120
S L T SSSSSS SKLIH YKHAINGF+A+LTPS+L+AL+ SPGYVSSVRDSSV+ DT
Sbjct: 61 SPLRTTTSSSSSS-PSKLIHCYKHAINGFTANLTPSQLDALKNSPGYVSSVRDSSVRADT 120
Query: 121 THSSHFLGLSSNSGLLPISNYGSDVIIGVVDTGVWPESGSFNDDGMFEIPSRWKGECESG 180
THSS+FL LS NSGLLPISNYGSDVIIG VDTGVWPES SFNDDG+ +IPSRWKGECESG
Sbjct: 121 THSSNFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESG 180
Query: 181 AHFSGSLCNKKLIGARFFNKGLIAKFPNITISMNSTRDTNGHGTHTSTTAAGSYVKEASF 240
HF+ SLCNKKLIG RFFNKGLIAKFPN+TISMNSTRDTNGHGTHTSTTAAGSYVKEASF
Sbjct: 181 THFNASLCNKKLIGGRFFNKGLIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASF 240
Query: 241 FGYGLGIARGVAPRARVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVDGVPLY 300
FGYG G ARGVAPRARVAIYKA+W EGN VSDVIAAID AI DGVDVISLSLG+DGVPLY
Sbjct: 241 FGYGQGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLY 300
Query: 301 EDPIAIATYAAMERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFG-------- 360
EDP+AIAT+AAMERGIFVATSAGN GPQ GTVH+GAPWVLNVAAGTMDRDFG
Sbjct: 301 EDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNG 360
Query: 361 ------------------------------------------------------------ 420
Sbjct: 361 VSVLGSSLFPLNLAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVE 420
Query: 421 --EVAVGVFISNISDWDNLIQTPFPSIFLSPYHGNIIKDYIKGNSDPKARVNFHKTILGT 480
++AVGVFISNISDWDNLIQT FPSIFL+ YHGN+IKDYIK +S+PKARVNFHKTI+GT
Sbjct: 421 SAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGT 480
Query: 481 KPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSG 540
KPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDV SRPIYSK+NVLSG
Sbjct: 481 KPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKYNVLSG 540
Query: 541 TSMSCPHAAGVAALLKGAHPRWSLAAIRSAMMTTADVLDNTQTPIKDLGDRNKLATPLAM 600
TSM+CPHAAGVAALLKGAHP WS AAIRSAMMTTAD++DNTQT IKDLG++NK+ATPLAM
Sbjct: 541 TSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAM 600
Query: 601 GSGHVNPNKAMDPGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLDLNYPS 660
GSGHVNPNKA+DPGL+YDMGI+DY NLLCALNYTKNQIQTITRSTSN+CE P LDLNYPS
Sbjct: 601 GSGHVNPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKPLLDLNYPS 660
Query: 661 FIIIVNASDSKATR-EISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKN 701
FII VNASDS+ R EISREF R VTNIGEKG TY+AK+ M GL VTV+P KLKFK KN
Sbjct: 661 FIITVNASDSQTGRTEISREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKN 720
BLAST of Spg025294 vs. NCBI nr
Match:
XP_022996869.1 (subtilisin-like protease SBT1.9 [Cucurbita maxima])
HSP 1 Score: 1095.9 bits (2833), Expect = 0.0e+00
Identity = 578/768 (75.26%), Postives = 625/768 (81.38%), Query Frame = 0
Query: 1 MAAHMCMYFWFSLMAIFWLPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSV-LDN 60
MAAHM M+F SLM I T TET+NYIVHMDLAAMPKPFA+HHSWYSATLSSV LD
Sbjct: 1 MAAHMRMFFCLSLMVILLARSTLTETDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDT 60
Query: 61 SRLGTMASSSSSSLSSKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDT 120
S L T +SSSSSL SKLIH YKHAINGF+A+LTPS+L+AL+ SPGYVSSVRDSSV+ DT
Sbjct: 61 SPLRT-TTSSSSSLPSKLIHCYKHAINGFTANLTPSQLDALKNSPGYVSSVRDSSVRADT 120
Query: 121 THSSHFLGLSSNSGLLPISNYGSDVIIGVVDTGVWPESGSFNDDGMFEIPSRWKGECESG 180
THSS+FL L+ NSGLLPISNYGSDVIIG VDTGVWPES SFND+ + +IPSRWKGECESG
Sbjct: 121 THSSNFLALTPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDEAISKIPSRWKGECESG 180
Query: 181 AHFSGSLCNKKLIGARFFNKGLIAKFPNITISMNSTRDTNGHGTHTSTTAAGSYVKEASF 240
HF+ SLCNKKLIG RFFNKGLIAKFPN+TISMNSTRDTNGHGTHTSTTAAGSYVKEASF
Sbjct: 181 THFNASLCNKKLIGGRFFNKGLIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASF 240
Query: 241 FGYGLGIARGVAPRARVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVDGVPLY 300
FGYG G ARGVAPRARVAIYKA+W EGN VSDVIAAID AI DGVDVISLSLG+DGVPLY
Sbjct: 241 FGYGQGTARGVAPRARVAIYKAIWKEGNVVSDVIAAIDQAISDGVDVISLSLGLDGVPLY 300
Query: 301 EDPIAIATYAAMERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFG-------- 360
EDP+AIAT+AAMERGIFVATSAGN GPQ GTVH+GAPWVLNVAAGTMDRDFG
Sbjct: 301 EDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLNNG 360
Query: 361 ------------------------------------------------------------ 420
Sbjct: 361 VSVLGSSLFVLNSAIVLSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVQ 420
Query: 421 --EVAVGVFISNISDWDNLIQTPFPSIFLSPYHGNIIKDYIKGNSDPKARVNFHKTILGT 480
++AVGVFISNISDWDNLIQT FPSIFL+ YHGN+IKDYI +S+PKARVNFHKTI+GT
Sbjct: 421 SAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIMRSSNPKARVNFHKTIIGT 480
Query: 481 KPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSG 540
KPAPSVARYSSRGPSESCP VLKPDIMAPGDAILASWPQ VAATDV SRPIYSKFNVLSG
Sbjct: 481 KPAPSVARYSSRGPSESCPLVLKPDIMAPGDAILASWPQKVAATDVYSRPIYSKFNVLSG 540
Query: 541 TSMSCPHAAGVAALLKGAHPRWSLAAIRSAMMTTADVLDNTQTPIKDLGDRNKLATPLAM 600
TSM+CPHAAGVAALLKGAHP WS AAIRSAMMTTADV+DNTQT IKDLG++NK+ATPLAM
Sbjct: 541 TSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADVVDNTQTLIKDLGNKNKVATPLAM 600
Query: 601 GSGHVNPNKAMDPGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLDLNYPS 660
GSGHVNPNKA+DPGL+YD+GI+DY NLLCALNYTKNQIQTITRS SN+CE PSLDLNYPS
Sbjct: 601 GSGHVNPNKAIDPGLIYDIGIEDYTNLLCALNYTKNQIQTITRSPSNDCEKPSLDLNYPS 660
Query: 661 FIIIVNASDSKA-TREISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKN 694
FII VNASDS++ REISREF R VTNIGEKG TY+AK+ M GL VTV+PK LKFK KN
Sbjct: 661 FIITVNASDSESGRREISREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPKMLKFKRKN 720
BLAST of Spg025294 vs. ExPASy Swiss-Prot
Match:
O82777 (Subtilisin-like protease SBT3 OS=Solanum lycopersicum OX=4081 GN=sbt3 PE=1 SV=1)
HSP 1 Score: 612.8 bits (1579), Expect = 4.7e-174
Identity = 343/767 (44.72%), Postives = 470/767 (61.28%), Query Frame = 0
Query: 4 HMCMYFWFSLMAIFWLPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSVLDNSRLG 63
H+ ++ W +F + YIVH+D + MP F HH W+S+T+ S+ +
Sbjct: 5 HLLLFSWALSAHLF---LALAQRSTYIVHLDKSLMPNVFTDHHHWHSSTIDSI----KAS 64
Query: 64 TMASSSSSSLSSKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSS 123
+S + KL++SY + ++GFSA L+ EL AL+K PG++S+ +D +V+ TTH+S
Sbjct: 65 VPSSVDRFHSAPKLVYSYDNVLHGFSAVLSKDELAALKKLPGFISAYKDRTVEPHTTHTS 124
Query: 124 HFLGLSSNSGLLPISNYGSDVIIGVVDTGVWPESGSFNDDGMFEIPSRWKGECESGAHFS 183
FL L+ +SGL P S G DVI+ V+D+G+WPES SF DDGM EIP RWKG C+ G F+
Sbjct: 125 DFLKLNPSSGLWPASGLGQDVIVAVLDSGIWPESASFQDDGMPEIPKRWKGICKPGTQFN 184
Query: 184 GSLCNKKLIGARFFNKGLIAKFPNITISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYG 243
S+CN+KLIGA +FNKG++A P + I+MNS RDT+GHGTH ++ AG++ K S FGY
Sbjct: 185 ASMCNRKLIGANYFNKGILANDPTVNITMNSARDTDGHGTHCASITAGNFAKGVSHFGYA 244
Query: 244 LGIARGVAPRARVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVDGVPLYEDPI 303
G ARGVAPRAR+A+YK + EG + SD+IAA+D A+ DGVD+IS+S G +PLYED I
Sbjct: 245 PGTARGVAPRARLAVYKFSFNEGTFTSDLIAAMDQAVADGVDMISISYGYRFIPLYEDAI 304
Query: 304 AIATYAAMERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDF-GEVAVG------ 363
+IA++ AM +G+ V+ SAGN GP +G+++NG+PW+L VA+G DR F G + +G
Sbjct: 305 SIASFGAMMKGVLVSASAGNRGPGIGSLNNGSPWILCVASGHTDRTFAGTLTLGNGLKIR 364
Query: 364 ----------------------------VFISNISDWDNLI------------------- 423
+S + + +N I
Sbjct: 365 GWSLFPARAFVRDSPVIYNKTLSDCSSEELLSQVENPENTIVICDDNGDFSDQMRIITRA 424
Query: 424 ------------------QTPFPSIFLSPYHGNIIKDYIKGNSDPKARVNFHKTILGTKP 483
P P + ++ G + +Y+K + P A + F +T L TKP
Sbjct: 425 RLKAAIFISEDPGVFRSATFPNPGVVVNKKEGKQVINYVKNSVTPTATITFQETYLDTKP 484
Query: 484 APSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVL-SGT 543
AP VA S+RGPS S + KPDI+APG ILA++P NV AT + + + S +L SGT
Sbjct: 485 APVVAASSARGPSRSYLGISKPDILAPGVLILAAYPPNVFATSIGTNILLSTDYILESGT 544
Query: 544 SMSCPHAAGVAALLKGAHPRWSLAAIRSAMMTTADVLDNTQTPIKDLGDRNKLATPLAMG 603
SM+ PHAAG+AA+LK AHP WS +AIRSAMMTTAD LDNT+ PIKD D NK ATPL MG
Sbjct: 545 SMAAPHAAGIAAMLKAAHPEWSPSAIRSAMMTTADPLDNTRKPIKD-SDNNKAATPLDMG 604
Query: 604 SGHVNPNKAMDPGLVYDMGIQDYVNLLCALNYTKNQIQTITRST-SNNCENPSLDLNYPS 663
+GHV+PN+A+DPGLVYD QDYVNLLC+LN+T+ Q +TI RS+ S+NC NPS DLNYPS
Sbjct: 605 AGHVDPNRALDPGLVYDATPQDYVNLLCSLNFTEEQFKTIARSSASHNCSNPSADLNYPS 664
Query: 664 FIIIVNASDSKATREISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKND 692
FI + + + E ++F R VTN+G+ TYKAKL A ++V P+ L FK KN+
Sbjct: 665 FIALYSIEGNFTLLE--QKFKRTVTNVGKGAATYKAKLKAPKNSTISVSPQILVFKNKNE 724
BLAST of Spg025294 vs. ExPASy Swiss-Prot
Match:
Q9FHA4 (Subtilisin-like protease SBT1.9 OS=Arabidopsis thaliana OX=3702 GN=SBT1.9 PE=2 SV=1)
HSP 1 Score: 556.2 bits (1432), Expect = 5.2e-157
Identity = 322/761 (42.31%), Postives = 442/761 (58.08%), Query Frame = 0
Query: 13 LMAIFWLPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSVLDNSRLGTMASSSSSS 72
L+ F++ ET YI+HMDL+A P PF+ H SW+S TL+SV+ N +
Sbjct: 9 LVFSFFVAIVTAETSPYIIHMDLSAKPLPFSDHRSWFSTTLTSVITNRK----------- 68
Query: 73 LSSKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSNS 132
K+I++Y +++GFSA LT SEL+ L+ PGYVS +D V++ TT S F+GL+S S
Sbjct: 69 --PKIIYAYTDSVHGFSAVLTNSELQRLKHKPGYVSFTKDLPVKLHTTFSPKFIGLNSTS 128
Query: 133 GLLPISNYGSDVIIGVVDTGVWPESGSFNDDGMFEIPSRWKGECESGAHFSGSLCNKKLI 192
G P+SNYG+ ++IG++DTG+WP+S SF+DDG+ +PS+WKG CE S SLCNKKLI
Sbjct: 129 GTWPVSNYGAGIVIGIIDTGIWPDSPSFHDDGVGSVPSKWKGACEFN---SSSLCNKKLI 188
Query: 193 GARFFNKGLIAKFPNITIS----MNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGLGIAR 252
GA+ FNKGL A P++ + +S DT GHGTH + AAG++VK AS+F Y G A
Sbjct: 189 GAKVFNKGLFANNPDLRETKIGQYSSPYDTIGHGTHVAAIAAGNHVKNASYFSYAQGTAS 248
Query: 253 GVAPRARVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGV--------DGVPLYE 312
G+AP A +AIYKA W EG Y SDVIAAID AI DGV VISLSLG+ DG L
Sbjct: 249 GIAPHAHLAIYKAAWEEGIYSSDVIAAIDQAIRDGVHVISLSLGLSFEDDDDNDGFGLEN 308
Query: 313 DPIAIATYAAMERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDF---------- 372
DPIA+A++AA+++G+FV TS GN+GP ++ NGAPW++ V AGT+ R F
Sbjct: 309 DPIAVASFAAIQKGVFVVTSGGNDGPYYWSLINGAPWIMTVGAGTIGRQFQGTLTFGNRV 368
Query: 373 -------------------------------------------------------GEVAV 432
G AV
Sbjct: 369 SFSFPSLFPGEFPSVQFPVTYIESGSVENKTLANRIVVCNENINIGSKLHQIRSTGAAAV 428
Query: 433 GVFISNISDWDNLIQTPFPSIFLSPYHGNIIKDYIKGN-SDPKARVNFHKTILGTKPAPS 492
+ + + + I+ FP F+ H I+ Y N ++ A++ F KT++GTKPAP
Sbjct: 429 VLITDKLLEEQDTIKFQFPVAFIGSKHRETIESYASSNKNNATAKLEFRKTVIGTKPAPE 488
Query: 493 VARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSC 552
V YSSRGP S P +LKPDI+APG IL++WP T + P++S FN+L+GTSM+
Sbjct: 489 VGTYSSRGPFTSFPQILKPDILAPGTLILSAWPSVEQITGTRALPLFSGFNLLTGTSMAA 548
Query: 553 PHAAGVAALLKGAHPRWSLAAIRSAMMTTADVLDNTQTPIKDLGDRNKLATPLAMGSGHV 612
PH AGVAAL+K HP WS +AI+SA+MTTA LDN PLA+G+GHV
Sbjct: 549 PHVAGVAALIKQVHPNWSPSAIKSAIMTTALTLDN----------------PLAVGAGHV 608
Query: 613 NPNKAMDPGLVYDMGIQDYVNLLC-ALNYTKNQIQTITRST-SNNCENPSLDLNYPSFII 672
+ NK ++PGL+YD QD++N LC ++ I ITRS S+ C+ PS LNYPS I
Sbjct: 609 STNKVLNPGLIYDTTPQDFINFLCHEAKQSRKLINIITRSNISDACKKPSPYLNYPSIIA 668
Query: 673 IVNASDSKATREISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKNDKLS 692
+ S + F R +TN+GE +Y ++ + GL V V+PKKL F KN+KLS
Sbjct: 669 YFTSDQSS-----PKIFKRTLTNVGEAKRSYIVRVRGLKGLNVVVEPKKLMFSEKNEKLS 728
BLAST of Spg025294 vs. ExPASy Swiss-Prot
Match:
Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)
HSP 1 Score: 503.8 bits (1296), Expect = 3.1e-141
Identity = 298/751 (39.68%), Postives = 418/751 (55.66%), Query Frame = 0
Query: 24 TETENYIVHMDLAAMPKPFASHHSWYSATLSSVLDNSRLGTMASSSSSSLSSKLIHSYKH 83
T + YI+ ++ + P+ F +HH WY++ L+S S L+++Y
Sbjct: 25 TAKKTYIIRVNHSDKPESFLTHHDWYTSQLNS------------------ESSLLYTYTT 84
Query: 84 AINGFSASLTPSELEALQKSPGYVSSV-RDSSVQVDTTHSSHFLGLSSNSGLLPISNYGS 143
+ +GFSA L +E ++L S + + D + TT + FLGL+S G+ + + +
Sbjct: 85 SFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSN 144
Query: 144 DVIIGVVDTGVWPESGSFNDDGMFEIPSRWKGECESGAHFSGSLCNKKLIGARFFNKGL- 203
VIIGV+DTGVWPES SF+D M EIPS+WKGECESG+ F LCNKKLIGAR F+KG
Sbjct: 145 GVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQ 204
Query: 204 IAKFPNITISMNST--RDTNGHGTHTSTTAAGSYVKEASFFGYGLGIARGVAPRARVAIY 263
+A + S RD +GHGTHTSTTAAGS V+ ASF GY G ARG+A RARVA Y
Sbjct: 205 MASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATY 264
Query: 264 KAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVDGVPLYEDPIAIATYAAMERGIFVAT 323
K W+ G + SD++AA+D AILDGVDV+SLSLG P Y D IAI ++AMERG+FV+
Sbjct: 265 KVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSC 324
Query: 324 SAGNNGPQLGTVHNGAPWVLNVAAGTMDRDF----------------------------- 383
SAGN+GP +V N APWV+ V AGT+DRDF
Sbjct: 325 SAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLE 384
Query: 384 -------------------------GEVAV---------------------GVFISN-IS 443
G++ V G+ ++N +
Sbjct: 385 LVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAA 444
Query: 444 DWDNLIQTP--FPSIFLSPYHGNIIKDYIKGNSDPKARVNFHKTILGTKPAPSVARYSSR 503
+ L+ P+I + G+++++Y+K +S P A + F T+L KP+P VA +SSR
Sbjct: 445 SGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSR 504
Query: 504 GPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSCPHAAGVA 563
GP+ P +LKPD++ PG ILA W + T ++ ++FN++SGTSMSCPH +G+A
Sbjct: 505 GPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLA 564
Query: 564 ALLKGAHPRWSLAAIRSAMMTTADVLDNTQTPIKDLGDRNKLATPLAMGSGHVNPNKAMD 623
LLK AHP WS +AI+SA+MTTA VLDNT P+ D D N L+ P A GSGHV+P KA+
Sbjct: 565 GLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAAD-NSLSNPYAHGSGHVDPQKALS 624
Query: 624 PGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLD---LNYPSFIIIVNASD 683
PGLVYD+ ++Y+ LC+L+YT + I I + S NC D LNYPSF ++
Sbjct: 625 PGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSVLFGG-- 684
Query: 684 SKATREISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKNDKLSFKLKI- 687
+ + R +TR VTN+G + YK + + ++VKP KL FK +K + +
Sbjct: 685 ----KRVVR-YTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFV 744
BLAST of Spg025294 vs. ExPASy Swiss-Prot
Match:
O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)
HSP 1 Score: 484.2 bits (1245), Expect = 2.5e-135
Identity = 294/752 (39.10%), Postives = 421/752 (55.98%), Query Frame = 0
Query: 29 YIVHMDLAAMPKPFASHHSWYSATLSSVLDNSRLGTMASSSSSSLSSKLIHSYKHAINGF 88
YIVHM + MP F H +WY ++L S+ D S++L+++Y++AI+GF
Sbjct: 32 YIVHMAKSQMPSSFDLHSNWYDSSLRSISD---------------SAELLYTYENAIHGF 91
Query: 89 SASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSNSG-LLPISNYGSDVIIG 148
S LT E ++L PG +S + + ++ TT + FLGL ++ L P + SDV++G
Sbjct: 92 STRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSDVVVG 151
Query: 149 VVDTGVWPESGSFNDDGMFEIPSRWKGECESGAHFSGSLCNKKLIGARFFNKGLIAKFPN 208
V+DTGVWPES S++D+G IPS WKG CE+G +F+ SLCN+KLIGARFF +G +
Sbjct: 152 VLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGP 211
Query: 209 ITIS--MNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGLGIARGVAPRARVAIYKAVWAE 268
I S S RD +GHGTHTS+TAAGS V+ AS GY G ARG+APRARVA+YK W
Sbjct: 212 IDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLG 271
Query: 269 GNYVSDVIAAIDHAILDGVDVISLSLGVDGVPLYEDPIAIATYAAMERGIFVATSAGNNG 328
G + SD++AAID AI D V+V+S+SLG Y D +AI +AAMERGI V+ SAGN G
Sbjct: 272 GCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAG 331
Query: 329 PQLGTVHNGAPWVLNVAAGTMDRDFGEVA------------------------------- 388
P ++ N APW+ V AGT+DRDF +A
Sbjct: 332 PSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGN 391
Query: 389 ----------------------------------------------VGVFISN-ISDWDN 448
VG+ ++N ++ +
Sbjct: 392 ASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEE 451
Query: 449 LIQTP--FPSIFLSPYHGNIIKDYIKGNSDPKARVNFHKTILGTKPAPSVARYSSRGPSE 508
L+ P+ + G+II+ Y+ + +P A ++ T++G KP+P VA +SSRGP+
Sbjct: 452 LVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNS 511
Query: 509 SCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSCPHAAGVAALLK 568
P +LKPD++APG ILA+W T + S +FN++SGTSMSCPH +G+AALLK
Sbjct: 512 ITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLK 571
Query: 569 GAHPRWSLAAIRSAMMTTADVLDNTQTPIKDLGDRNKLATPLAMGSGHVNPNKAMDPGLV 628
HP WS AAIRSA+MTTA P+ D+ K +TP G+GHV+P A +PGL+
Sbjct: 572 SVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIA-TGKPSTPFDHGAGHVSPTTATNPGLI 631
Query: 629 YDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPS-----LDLNYPSFIIIVNASDSK 688
YD+ +DY+ LCALNYT QI++++R N +PS DLNYPSF + V+
Sbjct: 632 YDLTTEDYLGFLCALNYTSPQIRSVSR--RNYTCDPSKSYSVADLNYPSFAVNVDGVG-- 691
Query: 689 ATREISREFTRMVTNIGEKGTTYKAKLIA-MDGLRVTVKPKKLKFKWKNDKLSFKL--KI 690
+ ++TR VT++G G TY K+ + G++++V+P L FK N+K S+ + +
Sbjct: 692 -----AYKYTRTVTSVGGAG-TYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTV 751
BLAST of Spg025294 vs. ExPASy Swiss-Prot
Match:
Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)
HSP 1 Score: 482.6 bits (1241), Expect = 7.3e-135
Identity = 306/791 (38.69%), Postives = 429/791 (54.24%), Query Frame = 0
Query: 5 MCMYFWFSLMAIFWLPFTFTETEN---YIVHMDLAAMPKPFASHHSWYSATLSSVLDNSR 64
M +F+F + P + + N YIVH+D A P F +H WY+++L+S+
Sbjct: 1 MAFFFYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASL----- 60
Query: 65 LGTMASSSSSSLSSKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTH 124
+S +IH+Y +GFSA LT + L P +S + + + TT
Sbjct: 61 ---------TSSPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTR 120
Query: 125 SSHFLGLSS--NSGLLPISNYGSDVIIGVVDTGVWPESGSFNDDGMFEIPSRWKGECESG 184
S FLGL S +GLL S++GSD++IGV+DTGVWPE SF+D G+ +P +WKG+C +
Sbjct: 121 SPEFLGLRSTDKAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIAS 180
Query: 185 AHFSGSLCNKKLIGARFFNKGLIAK--FPNITISMNSTRDTNGHGTHTSTTAAGSYVKEA 244
F S CN+KL+GARFF G A N T S RD++GHGTHT++ +AG YV A
Sbjct: 181 QDFPESACNRKLVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPA 240
Query: 245 SFFGYGLGIARGVAPRARVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVDGVP 304
S GY G+A G+AP+AR+A YK W G Y SD++AA D A+ DGVDVISLS+G VP
Sbjct: 241 STLGYAHGVAAGMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVP 300
Query: 305 LYEDPIAIATYAAMERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDF------- 364
Y D IAI + A++RGIFV+ SAGN GP TV N APW+ V AGT+DRDF
Sbjct: 301 YYLDAIAIGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLG 360
Query: 365 ------------------------------------------------------------ 424
Sbjct: 361 NGKMISGVSVYGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDR 420
Query: 425 --------GEV-----AVGVFISN-ISDWDNLIQT--PFPSIFLSPYHGNIIKDYI---- 484
GE+ +G+ I+N + D + L+ P+ + G+ I+ YI
Sbjct: 421 GINSRATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESS 480
Query: 485 --KGNSDPKARVNFHKTILGTKPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQ 544
+ + P A + F T LG +PAP VA +S+RGP+ P +LKPD++APG ILA+WP
Sbjct: 481 KSRSSKHPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPD 540
Query: 545 NVAATDVNSRPIYSKFNVLSGTSMSCPHAAGVAALLKGAHPRWSLAAIRSAMMTTADVLD 604
+ + V S ++FN+LSGTSM+CPH +G+AALLK AHP WS AAIRSA++TTA +D
Sbjct: 541 RIGPSGVTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVD 600
Query: 605 NTQTPIKDLGDRNKLATPLAMGSGHVNPNKAMDPGLVYDMGIQDYVNLLCALNYTKNQIQ 664
N+ P+ D N ++ + GSGHV+P KAMDPGLVYD+ DY+N LC NYT+ I
Sbjct: 601 NSGEPMMDESTGN-TSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIV 660
Query: 665 TITRSTSNNCENPSL-----DLNYPSFIIIVNASDSKATREISREFTRMVTNIGEKGTTY 690
TITR + +C+ +LNYPSF ++ ++S F R VTN+G+ + Y
Sbjct: 661 TITRRQA-DCDGARRAGHVGNLNYPSFSVVF---QQYGESKMSTHFIRTVTNVGDSDSVY 720
BLAST of Spg025294 vs. ExPASy TrEMBL
Match:
A0A6J1HH07 (subtilisin-like protease SBT1.9 OS=Cucurbita moschata OX=3662 GN=LOC111462861 PE=3 SV=1)
HSP 1 Score: 1110.9 bits (2872), Expect = 0.0e+00
Identity = 583/775 (75.23%), Postives = 634/775 (81.81%), Query Frame = 0
Query: 1 MAAHMCMYFWFSLMAIFWLPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSV-LDN 60
MAAHM M+FW SLM IF T T+T+NYIVHMDLAAMPKPFA+HHSWYSATLSSV LD
Sbjct: 1 MAAHMRMFFWVSLMVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDT 60
Query: 61 SRLGTMASSSSSSLSSKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDT 120
S L T SSSSSSL SKLIH YKHAINGF+ASLTPS+L+AL+ SPGYVSS+RDSSV+ DT
Sbjct: 61 SPLRTTTSSSSSSLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSSVRADT 120
Query: 121 THSSHFLGLSSNSGLLPISNYGSDVIIGVVDTGVWPESGSFNDDGMFEIPSRWKGECESG 180
THSS+FL LS NSGLLPISNYGSDVIIG VDTGVWPES SFNDDG+ +IPSRWKGECESG
Sbjct: 121 THSSNFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESG 180
Query: 181 AHFSGSLCNKKLIGARFFNKGLIAKFPNITISMNSTRDTNGHGTHTSTTAAGSYVKEASF 240
HF+ SLCNKKLIG RFFNKGLI+KFPN+TISMNSTRDTNGHGTHTSTTAAGSYVKEASF
Sbjct: 181 THFNASLCNKKLIGGRFFNKGLISKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASF 240
Query: 241 FGYGLGIARGVAPRARVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVDGVPLY 300
FGYG G ARGVAPRARVAIYKA+W EGN VSDVIAAID AI DGVDVISLSLG+DGVPLY
Sbjct: 241 FGYGQGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLY 300
Query: 301 EDPIAIATYAAMERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFG-------- 360
EDP+AIAT+AAMERGIFVATSAGN GPQ GTVH+GAPWVLNVAAGTMDRDFG
Sbjct: 301 EDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNG 360
Query: 361 ------------------------------------------------------------ 420
Sbjct: 361 VSVLGSSLFPLNSAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVE 420
Query: 421 --EVAVGVFISNISDWDNLIQTPFPSIFLSPYHGNIIKDYIKGNSDPKARVNFHKTILGT 480
++AVGVFISNISDWDNLIQT FPSIFL+ YHGN+IKDYIK +S+PKARVNFHKTI+GT
Sbjct: 421 SAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGT 480
Query: 481 KPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSG 540
KPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDV SRPIYSKFNVLSG
Sbjct: 481 KPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNVLSG 540
Query: 541 TSMSCPHAAGVAALLKGAHPRWSLAAIRSAMMTTADVLDNTQTPIKDLGDRNKLATPLAM 600
TSM+CPHAAGVAALLKGAHP WS AAIRSAMMTTAD++DNTQT IKDLG++NK+ATPLAM
Sbjct: 541 TSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAM 600
Query: 601 GSGHVNPNKAMDPGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLDLNYPS 660
GSGHVNPNKA+DPGL+YDMGI+DY NLLCALNYTKNQIQTITRSTSN+CE LDLNYPS
Sbjct: 601 GSGHVNPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKALLDLNYPS 660
Query: 661 FIIIVNASDSKATR-EISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKN 701
FI+ VNASDS+ R E+SREF R VTNIGEKG TY+AK+ M GL VTV+P KLKFK KN
Sbjct: 661 FIMTVNASDSQTGRTEMSREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKN 720
BLAST of Spg025294 vs. ExPASy TrEMBL
Match:
A0A6J1K9W2 (subtilisin-like protease SBT1.9 OS=Cucurbita maxima OX=3661 GN=LOC111491986 PE=3 SV=1)
HSP 1 Score: 1095.9 bits (2833), Expect = 0.0e+00
Identity = 578/768 (75.26%), Postives = 625/768 (81.38%), Query Frame = 0
Query: 1 MAAHMCMYFWFSLMAIFWLPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSV-LDN 60
MAAHM M+F SLM I T TET+NYIVHMDLAAMPKPFA+HHSWYSATLSSV LD
Sbjct: 1 MAAHMRMFFCLSLMVILLARSTLTETDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDT 60
Query: 61 SRLGTMASSSSSSLSSKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDT 120
S L T +SSSSSL SKLIH YKHAINGF+A+LTPS+L+AL+ SPGYVSSVRDSSV+ DT
Sbjct: 61 SPLRT-TTSSSSSLPSKLIHCYKHAINGFTANLTPSQLDALKNSPGYVSSVRDSSVRADT 120
Query: 121 THSSHFLGLSSNSGLLPISNYGSDVIIGVVDTGVWPESGSFNDDGMFEIPSRWKGECESG 180
THSS+FL L+ NSGLLPISNYGSDVIIG VDTGVWPES SFND+ + +IPSRWKGECESG
Sbjct: 121 THSSNFLALTPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDEAISKIPSRWKGECESG 180
Query: 181 AHFSGSLCNKKLIGARFFNKGLIAKFPNITISMNSTRDTNGHGTHTSTTAAGSYVKEASF 240
HF+ SLCNKKLIG RFFNKGLIAKFPN+TISMNSTRDTNGHGTHTSTTAAGSYVKEASF
Sbjct: 181 THFNASLCNKKLIGGRFFNKGLIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASF 240
Query: 241 FGYGLGIARGVAPRARVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVDGVPLY 300
FGYG G ARGVAPRARVAIYKA+W EGN VSDVIAAID AI DGVDVISLSLG+DGVPLY
Sbjct: 241 FGYGQGTARGVAPRARVAIYKAIWKEGNVVSDVIAAIDQAISDGVDVISLSLGLDGVPLY 300
Query: 301 EDPIAIATYAAMERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFG-------- 360
EDP+AIAT+AAMERGIFVATSAGN GPQ GTVH+GAPWVLNVAAGTMDRDFG
Sbjct: 301 EDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLNNG 360
Query: 361 ------------------------------------------------------------ 420
Sbjct: 361 VSVLGSSLFVLNSAIVLSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVQ 420
Query: 421 --EVAVGVFISNISDWDNLIQTPFPSIFLSPYHGNIIKDYIKGNSDPKARVNFHKTILGT 480
++AVGVFISNISDWDNLIQT FPSIFL+ YHGN+IKDYI +S+PKARVNFHKTI+GT
Sbjct: 421 SAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIMRSSNPKARVNFHKTIIGT 480
Query: 481 KPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSG 540
KPAPSVARYSSRGPSESCP VLKPDIMAPGDAILASWPQ VAATDV SRPIYSKFNVLSG
Sbjct: 481 KPAPSVARYSSRGPSESCPLVLKPDIMAPGDAILASWPQKVAATDVYSRPIYSKFNVLSG 540
Query: 541 TSMSCPHAAGVAALLKGAHPRWSLAAIRSAMMTTADVLDNTQTPIKDLGDRNKLATPLAM 600
TSM+CPHAAGVAALLKGAHP WS AAIRSAMMTTADV+DNTQT IKDLG++NK+ATPLAM
Sbjct: 541 TSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADVVDNTQTLIKDLGNKNKVATPLAM 600
Query: 601 GSGHVNPNKAMDPGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLDLNYPS 660
GSGHVNPNKA+DPGL+YD+GI+DY NLLCALNYTKNQIQTITRS SN+CE PSLDLNYPS
Sbjct: 601 GSGHVNPNKAIDPGLIYDIGIEDYTNLLCALNYTKNQIQTITRSPSNDCEKPSLDLNYPS 660
Query: 661 FIIIVNASDSKA-TREISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKN 694
FII VNASDS++ REISREF R VTNIGEKG TY+AK+ M GL VTV+PK LKFK KN
Sbjct: 661 FIITVNASDSESGRREISREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPKMLKFKRKN 720
BLAST of Spg025294 vs. ExPASy TrEMBL
Match:
A0A5A7V589 (Subtilisin-like protease SBT1.9 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00840 PE=3 SV=1)
HSP 1 Score: 1058.9 bits (2737), Expect = 9.1e-306
Identity = 546/758 (72.03%), Postives = 604/758 (79.68%), Query Frame = 0
Query: 7 MYFWFSLMAIFWLPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSVL-DNSRLGTM 66
+YFWFSL IFWL TET NYIVHM+ AAMPKPFAS HSWYSAT+SSVL +S +
Sbjct: 5 LYFWFSLTPIFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSSSSSS 64
Query: 67 ASSSSSSLSSKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHF 126
+SSSSSS SKLIH+Y HAI+GF ASLTPS+LEAL+ SPGY+SSV DSSV VDTTHSSHF
Sbjct: 65 SSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSHF 124
Query: 127 LGLSSNSGLLPISNYGSDVIIGVVDTGVWPESGSFNDDGMFEIPSRWKGECESGAHFSGS 186
LGLSSN GLLPIS YGSDVIIG VDTG+WP+S SFNDDGM EIPSRWKGECES HF+ S
Sbjct: 125 LGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRWKGECESSTHFNVS 184
Query: 187 LCNKKLIGARFFNKGLIAKFPNITISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGLG 246
CN KLIGARFFNKGLIA+FPN TISMNSTRDT GHGTHTST AAGSYVKEASFFGYG G
Sbjct: 185 FCNNKLIGARFFNKGLIAQFPNATISMNSTRDTIGHGTHTSTIAAGSYVKEASFFGYGRG 244
Query: 247 IARGVAPRARVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVDGVPLYEDPIAI 306
ARGVAPRARVAIYKA+W EGN VSDV+AAID AI DGVDVISLS+G+DG+PLY DP++I
Sbjct: 245 TARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIGIDGIPLYNDPVSI 304
Query: 307 ATYAAMERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFG-------------- 366
AT+AA+ERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFG
Sbjct: 305 ATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVSVLGS 364
Query: 367 --------------------------------------------------------EVAV 426
+V +
Sbjct: 365 SLFPLNTTMGLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTAKVTL 424
Query: 427 GVFISNISDWDNLIQTPFPSIFLSPYHGNIIKDYIKGNSDPKARVNFHKTILGTKPAPSV 486
G+FISNI DWDNLIQTPFPSIF++PYHGNIIKDYI +SDPKA VNFHKTILGTKPAP+V
Sbjct: 425 GIFISNIFDWDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNFHKTILGTKPAPTV 484
Query: 487 ARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSCP 546
ARYSSRGPS+SCPFVLKPDIMAPGDAILASWPQN+ A DVNS PIY+KFNV+SGTSMSCP
Sbjct: 485 ARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYTKFNVISGTSMSCP 544
Query: 547 HAAGVAALLKGAHPRWSLAAIRSAMMTTADVLDNTQTPIKDLGDRNKLATPLAMGSGHVN 606
HAAGVAALLKGAHP+WS AAIRSAMMTTADV+DNTQT IKD+G+ NK ATPLAMGSGHVN
Sbjct: 545 HAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNKFATPLAMGSGHVN 604
Query: 607 PNKAMDPGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLDLNYPSFIIIVN 666
PNKA+DP L+YD+GIQDYVN+LCALNYT+NQI+ ITRS SNNCENPSLDLNYPSFIII N
Sbjct: 605 PNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFIIIAN 664
Query: 667 ASDSK-ATREISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKNDKLSFK 693
+SDSK R+I EF R +T IGE TY+AKL M G +V VKP+ L+FK KN KLSF+
Sbjct: 665 SSDSKTGKRKILGEFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRILEFKRKNQKLSFE 724
BLAST of Spg025294 vs. ExPASy TrEMBL
Match:
A0A1S3BBC3 (subtilisin-like protease SBT1.9 OS=Cucumis melo OX=3656 GN=LOC103487840 PE=3 SV=1)
HSP 1 Score: 1058.9 bits (2737), Expect = 9.1e-306
Identity = 546/758 (72.03%), Postives = 604/758 (79.68%), Query Frame = 0
Query: 7 MYFWFSLMAIFWLPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSVL-DNSRLGTM 66
+YFWFSL IFWL TET NYIVHM+ AAMPKPFAS HSWYSAT+SSVL +S +
Sbjct: 5 LYFWFSLTPIFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSSSSSS 64
Query: 67 ASSSSSSLSSKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHF 126
+SSSSSS SKLIH+Y HAI+GF ASLTPS+LEAL+ SPGY+SSV DSSV VDTTHSSHF
Sbjct: 65 SSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSHF 124
Query: 127 LGLSSNSGLLPISNYGSDVIIGVVDTGVWPESGSFNDDGMFEIPSRWKGECESGAHFSGS 186
LGLSSN GLLPIS YGSDVIIG VDTG+WP+S SFNDDGM EIPSRWKGECES HF+ S
Sbjct: 125 LGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRWKGECESSTHFNVS 184
Query: 187 LCNKKLIGARFFNKGLIAKFPNITISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGLG 246
CN KLIGARFFNKGLIA+FPN TISMNSTRDT GHGTHTST AAGSYVKEASFFGYG G
Sbjct: 185 FCNNKLIGARFFNKGLIAQFPNATISMNSTRDTIGHGTHTSTIAAGSYVKEASFFGYGRG 244
Query: 247 IARGVAPRARVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVDGVPLYEDPIAI 306
ARGVAPRARVAIYKA+W EGN VSDV+AAID AI DGVDVISLS+G+DG+PLY DP++I
Sbjct: 245 TARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIGIDGIPLYNDPVSI 304
Query: 307 ATYAAMERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFG-------------- 366
AT+AA+ERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFG
Sbjct: 305 ATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVSVLGS 364
Query: 367 --------------------------------------------------------EVAV 426
+V +
Sbjct: 365 SLFPLNTTMGLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTAKVTL 424
Query: 427 GVFISNISDWDNLIQTPFPSIFLSPYHGNIIKDYIKGNSDPKARVNFHKTILGTKPAPSV 486
G+FISNI DWDNLIQTPFPSIF++PYHGNIIKDYI +SDPKA VNFHKTILGTKPAP+V
Sbjct: 425 GIFISNIFDWDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNFHKTILGTKPAPTV 484
Query: 487 ARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSCP 546
ARYSSRGPS+SCPFVLKPDIMAPGDAILASWPQN+ A DVNS PIY+KFNV+SGTSMSCP
Sbjct: 485 ARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYTKFNVISGTSMSCP 544
Query: 547 HAAGVAALLKGAHPRWSLAAIRSAMMTTADVLDNTQTPIKDLGDRNKLATPLAMGSGHVN 606
HAAGVAALLKGAHP+WS AAIRSAMMTTADV+DNTQT IKD+G+ NK ATPLAMGSGHVN
Sbjct: 545 HAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNKFATPLAMGSGHVN 604
Query: 607 PNKAMDPGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLDLNYPSFIIIVN 666
PNKA+DP L+YD+GIQDYVN+LCALNYT+NQI+ ITRS SNNCENPSLDLNYPSFIII N
Sbjct: 605 PNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFIIIAN 664
Query: 667 ASDSK-ATREISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKNDKLSFK 693
+SDSK R+I EF R +T IGE TY+AKL M G +V VKP+ L+FK KN KLSF+
Sbjct: 665 SSDSKTGKRKILGEFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRILEFKRKNQKLSFE 724
BLAST of Spg025294 vs. ExPASy TrEMBL
Match:
A0A6J1BPR9 (subtilisin-like protease SBT1.9 OS=Momordica charantia OX=3673 GN=LOC111004540 PE=3 SV=1)
HSP 1 Score: 1053.5 bits (2723), Expect = 3.8e-304
Identity = 554/777 (71.30%), Postives = 612/777 (78.76%), Query Frame = 0
Query: 4 HMCMYFWFSLMAIFW--LPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSVLDNSR 63
H C YFWF +AIFW LP TET+NYIVHMDLAAMPKPFA+HH+WYSATLSSVLDN R
Sbjct: 8 HNCFYFWFLFIAIFWFLLPSASTETDNYIVHMDLAAMPKPFATHHTWYSATLSSVLDNPR 67
Query: 64 LGTMASSSSSSLSSKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTH 123
LG L SKLIH+Y HAINGFSAS+TPSEL+ALQKSPGYVSSV DSS+QVDTTH
Sbjct: 68 LG--------MLPSKLIHTYNHAINGFSASITPSELKALQKSPGYVSSVPDSSLQVDTTH 127
Query: 124 SSHFLGLSSNSGLLPISNYGSDVIIGVVDTGVWPESGSFNDDGMFEIPSRWKGECESGAH 183
SSHFLGLSS+SG+L +SNYGSDVIIG VDTGVW ES SF+D+GM EIPSRWKG+CESG H
Sbjct: 128 SSHFLGLSSDSGVL-LSNYGSDVIIGFVDTGVWAESESFSDEGMPEIPSRWKGKCESGTH 187
Query: 184 FSGSLCNKKLIGARFFNKGLIAKFPNITISMNSTRDTNGHGTHTSTTAAGSYVKEASFFG 243
CNKKLIGARFFN+GLIAKFPN+TISMN T DTNGHGTHT+ TAAG YVK+ASFFG
Sbjct: 188 -----CNKKLIGARFFNRGLIAKFPNVTISMNYTNDTNGHGTHTAATAAGGYVKDASFFG 247
Query: 244 YGLGIARGVAPRARVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVDGVPLYED 303
YG G ARGVAPRARVAIYKA+W EGNYVSDVIAAID AILD VDV+SLSLG+DG LYED
Sbjct: 248 YGRGTARGVAPRARVAIYKAIWEEGNYVSDVIAAIDQAILDSVDVLSLSLGLDGALLYED 307
Query: 304 PIAIATYAAMERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFG---------- 363
P+AIAT+AA+ERGI V TSAGN GPQLGTVHNGAPWVLNVAAGTMDRDFG
Sbjct: 308 PVAIATFAAVERGILVTTSAGNKGPQLGTVHNGAPWVLNVAAGTMDRDFGGTIRLGNGVS 367
Query: 364 ------------------------------------------------------------ 423
Sbjct: 368 VLGSSLFPLNSGMATSKLPLVFMGGCYNLKKLKKVGHKIVVCEDKDEYNSYSLSLQVDNV 427
Query: 424 ---EVAVGVFISNISDWDNLIQTPFPSIFLSPYHGNIIKDYIKGNSDPKARVNFHKTILG 483
+V VGVFISN+SDWDN +QT FPSIFLSP +GNIIK YI+ +S+PKARV+FHKTI G
Sbjct: 428 ETAKVGVGVFISNLSDWDNSVQTSFPSIFLSPSNGNIIKHYIRTSSNPKARVSFHKTIFG 487
Query: 484 TKPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLS 543
KPAPSVARYSSRGP+ESCPFVLKPDIMAPGDAILASWP VAATDVNSRPIYSKFNVLS
Sbjct: 488 RKPAPSVARYSSRGPAESCPFVLKPDIMAPGDAILASWPLKVAATDVNSRPIYSKFNVLS 547
Query: 544 GTSMSCPHAAGVAALLKGAHPRWSLAAIRSAMMTTADVLDNTQTPIKDLGDRNKLATPLA 603
GTSMSCPHAAGVAALLK AHPRWS AAIRSAMMTTADV+DNTQTPIKDLG++NKLATPLA
Sbjct: 548 GTSMSCPHAAGVAALLKAAHPRWSPAAIRSAMMTTADVVDNTQTPIKDLGEKNKLATPLA 607
Query: 604 MGSGHVNPNKAMDPGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLDLNYP 663
MGSGHVNPNKA+DPGL+YDM IQDYVN+LCALNY KNQIQTITRS SNNC+NPSLDLNYP
Sbjct: 608 MGSGHVNPNKAVDPGLIYDMEIQDYVNVLCALNYNKNQIQTITRSASNNCKNPSLDLNYP 667
Query: 664 SFIIIVNASDSKATREISREFTRMVTNIGEKGTTYKAKLIAM--DGLRVTVKPKKLKFKW 700
SFI+IVNASDS EISREF R VTN+ E+ TTY+AKL M +G+RVTVKP KL+FK
Sbjct: 668 SFIVIVNASDSNTEMEISREFKRTVTNVVEEATTYEAKLTPMNGNGVRVTVKPHKLEFKG 727
BLAST of Spg025294 vs. TAIR 10
Match:
AT5G67090.1 (Subtilisin-like serine endopeptidase family protein )
HSP 1 Score: 556.2 bits (1432), Expect = 3.7e-158
Identity = 322/761 (42.31%), Postives = 442/761 (58.08%), Query Frame = 0
Query: 13 LMAIFWLPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSVLDNSRLGTMASSSSSS 72
L+ F++ ET YI+HMDL+A P PF+ H SW+S TL+SV+ N +
Sbjct: 9 LVFSFFVAIVTAETSPYIIHMDLSAKPLPFSDHRSWFSTTLTSVITNRK----------- 68
Query: 73 LSSKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSNS 132
K+I++Y +++GFSA LT SEL+ L+ PGYVS +D V++ TT S F+GL+S S
Sbjct: 69 --PKIIYAYTDSVHGFSAVLTNSELQRLKHKPGYVSFTKDLPVKLHTTFSPKFIGLNSTS 128
Query: 133 GLLPISNYGSDVIIGVVDTGVWPESGSFNDDGMFEIPSRWKGECESGAHFSGSLCNKKLI 192
G P+SNYG+ ++IG++DTG+WP+S SF+DDG+ +PS+WKG CE S SLCNKKLI
Sbjct: 129 GTWPVSNYGAGIVIGIIDTGIWPDSPSFHDDGVGSVPSKWKGACEFN---SSSLCNKKLI 188
Query: 193 GARFFNKGLIAKFPNITIS----MNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGLGIAR 252
GA+ FNKGL A P++ + +S DT GHGTH + AAG++VK AS+F Y G A
Sbjct: 189 GAKVFNKGLFANNPDLRETKIGQYSSPYDTIGHGTHVAAIAAGNHVKNASYFSYAQGTAS 248
Query: 253 GVAPRARVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGV--------DGVPLYE 312
G+AP A +AIYKA W EG Y SDVIAAID AI DGV VISLSLG+ DG L
Sbjct: 249 GIAPHAHLAIYKAAWEEGIYSSDVIAAIDQAIRDGVHVISLSLGLSFEDDDDNDGFGLEN 308
Query: 313 DPIAIATYAAMERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDF---------- 372
DPIA+A++AA+++G+FV TS GN+GP ++ NGAPW++ V AGT+ R F
Sbjct: 309 DPIAVASFAAIQKGVFVVTSGGNDGPYYWSLINGAPWIMTVGAGTIGRQFQGTLTFGNRV 368
Query: 373 -------------------------------------------------------GEVAV 432
G AV
Sbjct: 369 SFSFPSLFPGEFPSVQFPVTYIESGSVENKTLANRIVVCNENINIGSKLHQIRSTGAAAV 428
Query: 433 GVFISNISDWDNLIQTPFPSIFLSPYHGNIIKDYIKGN-SDPKARVNFHKTILGTKPAPS 492
+ + + + I+ FP F+ H I+ Y N ++ A++ F KT++GTKPAP
Sbjct: 429 VLITDKLLEEQDTIKFQFPVAFIGSKHRETIESYASSNKNNATAKLEFRKTVIGTKPAPE 488
Query: 493 VARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSC 552
V YSSRGP S P +LKPDI+APG IL++WP T + P++S FN+L+GTSM+
Sbjct: 489 VGTYSSRGPFTSFPQILKPDILAPGTLILSAWPSVEQITGTRALPLFSGFNLLTGTSMAA 548
Query: 553 PHAAGVAALLKGAHPRWSLAAIRSAMMTTADVLDNTQTPIKDLGDRNKLATPLAMGSGHV 612
PH AGVAAL+K HP WS +AI+SA+MTTA LDN PLA+G+GHV
Sbjct: 549 PHVAGVAALIKQVHPNWSPSAIKSAIMTTALTLDN----------------PLAVGAGHV 608
Query: 613 NPNKAMDPGLVYDMGIQDYVNLLC-ALNYTKNQIQTITRST-SNNCENPSLDLNYPSFII 672
+ NK ++PGL+YD QD++N LC ++ I ITRS S+ C+ PS LNYPS I
Sbjct: 609 STNKVLNPGLIYDTTPQDFINFLCHEAKQSRKLINIITRSNISDACKKPSPYLNYPSIIA 668
Query: 673 IVNASDSKATREISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKNDKLS 692
+ S + F R +TN+GE +Y ++ + GL V V+PKKL F KN+KLS
Sbjct: 669 YFTSDQSS-----PKIFKRTLTNVGEAKRSYIVRVRGLKGLNVVVEPKKLMFSEKNEKLS 728
BLAST of Spg025294 vs. TAIR 10
Match:
AT2G05920.1 (Subtilase family protein )
HSP 1 Score: 503.8 bits (1296), Expect = 2.2e-142
Identity = 298/751 (39.68%), Postives = 418/751 (55.66%), Query Frame = 0
Query: 24 TETENYIVHMDLAAMPKPFASHHSWYSATLSSVLDNSRLGTMASSSSSSLSSKLIHSYKH 83
T + YI+ ++ + P+ F +HH WY++ L+S S L+++Y
Sbjct: 25 TAKKTYIIRVNHSDKPESFLTHHDWYTSQLNS------------------ESSLLYTYTT 84
Query: 84 AINGFSASLTPSELEALQKSPGYVSSV-RDSSVQVDTTHSSHFLGLSSNSGLLPISNYGS 143
+ +GFSA L +E ++L S + + D + TT + FLGL+S G+ + + +
Sbjct: 85 SFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSN 144
Query: 144 DVIIGVVDTGVWPESGSFNDDGMFEIPSRWKGECESGAHFSGSLCNKKLIGARFFNKGL- 203
VIIGV+DTGVWPES SF+D M EIPS+WKGECESG+ F LCNKKLIGAR F+KG
Sbjct: 145 GVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQ 204
Query: 204 IAKFPNITISMNST--RDTNGHGTHTSTTAAGSYVKEASFFGYGLGIARGVAPRARVAIY 263
+A + S RD +GHGTHTSTTAAGS V+ ASF GY G ARG+A RARVA Y
Sbjct: 205 MASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATY 264
Query: 264 KAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVDGVPLYEDPIAIATYAAMERGIFVAT 323
K W+ G + SD++AA+D AILDGVDV+SLSLG P Y D IAI ++AMERG+FV+
Sbjct: 265 KVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSC 324
Query: 324 SAGNNGPQLGTVHNGAPWVLNVAAGTMDRDF----------------------------- 383
SAGN+GP +V N APWV+ V AGT+DRDF
Sbjct: 325 SAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLE 384
Query: 384 -------------------------GEVAV---------------------GVFISN-IS 443
G++ V G+ ++N +
Sbjct: 385 LVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAA 444
Query: 444 DWDNLIQTP--FPSIFLSPYHGNIIKDYIKGNSDPKARVNFHKTILGTKPAPSVARYSSR 503
+ L+ P+I + G+++++Y+K +S P A + F T+L KP+P VA +SSR
Sbjct: 445 SGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSR 504
Query: 504 GPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSCPHAAGVA 563
GP+ P +LKPD++ PG ILA W + T ++ ++FN++SGTSMSCPH +G+A
Sbjct: 505 GPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLA 564
Query: 564 ALLKGAHPRWSLAAIRSAMMTTADVLDNTQTPIKDLGDRNKLATPLAMGSGHVNPNKAMD 623
LLK AHP WS +AI+SA+MTTA VLDNT P+ D D N L+ P A GSGHV+P KA+
Sbjct: 565 GLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAAD-NSLSNPYAHGSGHVDPQKALS 624
Query: 624 PGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLD---LNYPSFIIIVNASD 683
PGLVYD+ ++Y+ LC+L+YT + I I + S NC D LNYPSF ++
Sbjct: 625 PGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSVLFGG-- 684
Query: 684 SKATREISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKNDKLSFKLKI- 687
+ + R +TR VTN+G + YK + + ++VKP KL FK +K + +
Sbjct: 685 ----KRVVR-YTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFV 744
BLAST of Spg025294 vs. TAIR 10
Match:
AT5G67360.1 (Subtilase family protein )
HSP 1 Score: 484.2 bits (1245), Expect = 1.8e-136
Identity = 294/752 (39.10%), Postives = 421/752 (55.98%), Query Frame = 0
Query: 29 YIVHMDLAAMPKPFASHHSWYSATLSSVLDNSRLGTMASSSSSSLSSKLIHSYKHAINGF 88
YIVHM + MP F H +WY ++L S+ D S++L+++Y++AI+GF
Sbjct: 32 YIVHMAKSQMPSSFDLHSNWYDSSLRSISD---------------SAELLYTYENAIHGF 91
Query: 89 SASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSNSG-LLPISNYGSDVIIG 148
S LT E ++L PG +S + + ++ TT + FLGL ++ L P + SDV++G
Sbjct: 92 STRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSDVVVG 151
Query: 149 VVDTGVWPESGSFNDDGMFEIPSRWKGECESGAHFSGSLCNKKLIGARFFNKGLIAKFPN 208
V+DTGVWPES S++D+G IPS WKG CE+G +F+ SLCN+KLIGARFF +G +
Sbjct: 152 VLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGP 211
Query: 209 ITIS--MNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGLGIARGVAPRARVAIYKAVWAE 268
I S S RD +GHGTHTS+TAAGS V+ AS GY G ARG+APRARVA+YK W
Sbjct: 212 IDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLG 271
Query: 269 GNYVSDVIAAIDHAILDGVDVISLSLGVDGVPLYEDPIAIATYAAMERGIFVATSAGNNG 328
G + SD++AAID AI D V+V+S+SLG Y D +AI +AAMERGI V+ SAGN G
Sbjct: 272 GCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAG 331
Query: 329 PQLGTVHNGAPWVLNVAAGTMDRDFGEVA------------------------------- 388
P ++ N APW+ V AGT+DRDF +A
Sbjct: 332 PSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGN 391
Query: 389 ----------------------------------------------VGVFISN-ISDWDN 448
VG+ ++N ++ +
Sbjct: 392 ASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEE 451
Query: 449 LIQTP--FPSIFLSPYHGNIIKDYIKGNSDPKARVNFHKTILGTKPAPSVARYSSRGPSE 508
L+ P+ + G+II+ Y+ + +P A ++ T++G KP+P VA +SSRGP+
Sbjct: 452 LVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNS 511
Query: 509 SCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSCPHAAGVAALLK 568
P +LKPD++APG ILA+W T + S +FN++SGTSMSCPH +G+AALLK
Sbjct: 512 ITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLK 571
Query: 569 GAHPRWSLAAIRSAMMTTADVLDNTQTPIKDLGDRNKLATPLAMGSGHVNPNKAMDPGLV 628
HP WS AAIRSA+MTTA P+ D+ K +TP G+GHV+P A +PGL+
Sbjct: 572 SVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIA-TGKPSTPFDHGAGHVSPTTATNPGLI 631
Query: 629 YDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPS-----LDLNYPSFIIIVNASDSK 688
YD+ +DY+ LCALNYT QI++++R N +PS DLNYPSF + V+
Sbjct: 632 YDLTTEDYLGFLCALNYTSPQIRSVSR--RNYTCDPSKSYSVADLNYPSFAVNVDGVG-- 691
Query: 689 ATREISREFTRMVTNIGEKGTTYKAKLIA-MDGLRVTVKPKKLKFKWKNDKLSFKL--KI 690
+ ++TR VT++G G TY K+ + G++++V+P L FK N+K S+ + +
Sbjct: 692 -----AYKYTRTVTSVGGAG-TYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTV 751
BLAST of Spg025294 vs. TAIR 10
Match:
AT3G14240.1 (Subtilase family protein )
HSP 1 Score: 482.6 bits (1241), Expect = 5.2e-136
Identity = 306/791 (38.69%), Postives = 429/791 (54.24%), Query Frame = 0
Query: 5 MCMYFWFSLMAIFWLPFTFTETEN---YIVHMDLAAMPKPFASHHSWYSATLSSVLDNSR 64
M +F+F + P + + N YIVH+D A P F +H WY+++L+S+
Sbjct: 1 MAFFFYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASL----- 60
Query: 65 LGTMASSSSSSLSSKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTH 124
+S +IH+Y +GFSA LT + L P +S + + + TT
Sbjct: 61 ---------TSSPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTR 120
Query: 125 SSHFLGLSS--NSGLLPISNYGSDVIIGVVDTGVWPESGSFNDDGMFEIPSRWKGECESG 184
S FLGL S +GLL S++GSD++IGV+DTGVWPE SF+D G+ +P +WKG+C +
Sbjct: 121 SPEFLGLRSTDKAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIAS 180
Query: 185 AHFSGSLCNKKLIGARFFNKGLIAK--FPNITISMNSTRDTNGHGTHTSTTAAGSYVKEA 244
F S CN+KL+GARFF G A N T S RD++GHGTHT++ +AG YV A
Sbjct: 181 QDFPESACNRKLVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPA 240
Query: 245 SFFGYGLGIARGVAPRARVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVDGVP 304
S GY G+A G+AP+AR+A YK W G Y SD++AA D A+ DGVDVISLS+G VP
Sbjct: 241 STLGYAHGVAAGMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVP 300
Query: 305 LYEDPIAIATYAAMERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDF------- 364
Y D IAI + A++RGIFV+ SAGN GP TV N APW+ V AGT+DRDF
Sbjct: 301 YYLDAIAIGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLG 360
Query: 365 ------------------------------------------------------------ 424
Sbjct: 361 NGKMISGVSVYGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDR 420
Query: 425 --------GEV-----AVGVFISN-ISDWDNLIQT--PFPSIFLSPYHGNIIKDYI---- 484
GE+ +G+ I+N + D + L+ P+ + G+ I+ YI
Sbjct: 421 GINSRATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESS 480
Query: 485 --KGNSDPKARVNFHKTILGTKPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQ 544
+ + P A + F T LG +PAP VA +S+RGP+ P +LKPD++APG ILA+WP
Sbjct: 481 KSRSSKHPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPD 540
Query: 545 NVAATDVNSRPIYSKFNVLSGTSMSCPHAAGVAALLKGAHPRWSLAAIRSAMMTTADVLD 604
+ + V S ++FN+LSGTSM+CPH +G+AALLK AHP WS AAIRSA++TTA +D
Sbjct: 541 RIGPSGVTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVD 600
Query: 605 NTQTPIKDLGDRNKLATPLAMGSGHVNPNKAMDPGLVYDMGIQDYVNLLCALNYTKNQIQ 664
N+ P+ D N ++ + GSGHV+P KAMDPGLVYD+ DY+N LC NYT+ I
Sbjct: 601 NSGEPMMDESTGN-TSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIV 660
Query: 665 TITRSTSNNCENPSL-----DLNYPSFIIIVNASDSKATREISREFTRMVTNIGEKGTTY 690
TITR + +C+ +LNYPSF ++ ++S F R VTN+G+ + Y
Sbjct: 661 TITRRQA-DCDGARRAGHVGNLNYPSFSVVF---QQYGESKMSTHFIRTVTNVGDSDSVY 720
BLAST of Spg025294 vs. TAIR 10
Match:
AT4G34980.1 (subtilisin-like serine protease 2 )
HSP 1 Score: 480.7 bits (1236), Expect = 2.0e-135
Identity = 303/780 (38.85%), Postives = 421/780 (53.97%), Query Frame = 0
Query: 12 SLMAIFWLPFTFTE------TENYIVHMDLAAMPKPFASHHSWYSATLSSVLDNSRLGTM 71
+++ + +L F F + +I +D +MP F +H+ WYS +
Sbjct: 5 TIVLLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAE---------- 64
Query: 72 ASSSSSSLSSKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHF 131
S+++H Y +GFSA +TP E + L+ P ++ D ++ TT S F
Sbjct: 65 --------ESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQF 124
Query: 132 LGLSSNSGLLPISNYGSDVIIGVVDTGVWPESGSFNDDGMFEIPSRWKGECESGAHFSGS 191
LGL + GL S+YGSDVIIGV DTG+WPE SF+D + IP RW+G CESGA FS
Sbjct: 125 LGLQNQKGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPR 184
Query: 192 LCNKKLIGARFFNKGLIAKF---PNITISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGY 251
CN+K+IGARFF KG A N T+ S RD +GHGTHTS+TAAG + +AS GY
Sbjct: 185 NCNRKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGY 244
Query: 252 GLGIARGVAPRARVAIYKAVWAE-GNYVSDVIAAIDHAILDGVDVISLSL-GVDGV--PL 311
G+A+GVAP+AR+A YK W + G SD++AA D A+ DGVDVIS+S+ G DG+ P
Sbjct: 245 ASGVAKGVAPKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPY 304
Query: 312 YEDPIAIATYAAMERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDF-------- 371
Y DPIAI +Y A +GIFV++SAGN GP +V N APWV V A T+DR+F
Sbjct: 305 YLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGD 364
Query: 372 -----------------------------------------------GEV---------- 431
G++
Sbjct: 365 GHRLRGVSLYAGVPLNGRMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPR 424
Query: 432 -----------AVGVFISN-ISDWDNLIQTP--FPSIFLSPYHGNIIKDYIKGNSDPKAR 491
VG+ ++N S+ + L+ P+ + G+ IK Y + +P A
Sbjct: 425 VAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIAS 484
Query: 492 VNFHKTILGTKPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRP 551
++F TI+G KPAP +A +S RGP+ P +LKPD++APG ILA+W V T + S P
Sbjct: 485 IDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDP 544
Query: 552 IYSKFNVLSGTSMSCPHAAGVAALLKGAHPRWSLAAIRSAMMTTADVLDNTQTPIKDLGD 611
++FN+LSGTSM+CPH +G AALLK AHP WS A IRSAMMTT +++DN+ + D
Sbjct: 545 RKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLID-ES 604
Query: 612 RNKLATPLAMGSGHVNPNKAMDPGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSN--N 671
K ATP GSGH+N +AM+PGLVYD+ DY+ LC++ Y IQ ITR+
Sbjct: 605 TGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPT 664
Query: 672 CENPSL-DLNYPSFIIIVNASDSKATREISREFTRMVTNIGEKGTTYKAKLIAMDGLRVT 692
PS +LNYPS I + +S+ R TN+G+ Y+A++ + G+ VT
Sbjct: 665 TRKPSPGNLNYPS---ITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVT 724
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_023546329.1 | 0.0e+00 | 75.91 | subtilisin-like protease SBT1.9 [Cucurbita pepo subsp. pepo] | [more] |
| XP_022962414.1 | 0.0e+00 | 75.23 | subtilisin-like protease SBT1.9 [Cucurbita moschata] | [more] |
| KAG6598336.1 | 0.0e+00 | 75.35 | Subtilisin-like protease 3, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
| KAG7029306.1 | 0.0e+00 | 75.23 | Subtilisin-like protease SBT1.9, partial [Cucurbita argyrosperma subsp. argyrosp... | [more] |
| XP_022996869.1 | 0.0e+00 | 75.26 | subtilisin-like protease SBT1.9 [Cucurbita maxima] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| O82777 | 4.7e-174 | 44.72 | Subtilisin-like protease SBT3 OS=Solanum lycopersicum OX=4081 GN=sbt3 PE=1 SV=1 | [more] |
| Q9FHA4 | 5.2e-157 | 42.31 | Subtilisin-like protease SBT1.9 OS=Arabidopsis thaliana OX=3702 GN=SBT1.9 PE=2 S... | [more] |
| Q9ZUF6 | 3.1e-141 | 39.68 | Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 S... | [more] |
| O65351 | 2.5e-135 | 39.10 | Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... | [more] |
| Q9LUM3 | 7.3e-135 | 38.69 | Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 S... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1HH07 | 0.0e+00 | 75.23 | subtilisin-like protease SBT1.9 OS=Cucurbita moschata OX=3662 GN=LOC111462861 PE... | [more] |
| A0A6J1K9W2 | 0.0e+00 | 75.26 | subtilisin-like protease SBT1.9 OS=Cucurbita maxima OX=3661 GN=LOC111491986 PE=3... | [more] |
| A0A5A7V589 | 9.1e-306 | 72.03 | Subtilisin-like protease SBT1.9 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... | [more] |
| A0A1S3BBC3 | 9.1e-306 | 72.03 | subtilisin-like protease SBT1.9 OS=Cucumis melo OX=3656 GN=LOC103487840 PE=3 SV=... | [more] |
| A0A6J1BPR9 | 3.8e-304 | 71.30 | subtilisin-like protease SBT1.9 OS=Momordica charantia OX=3673 GN=LOC111004540 P... | [more] |
