Spg025258 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg025258 vs. NCBI nr
Match: XP_022153121.1 (transport and Golgi organization 2 homolog [Momordica charantia] >XP_022153123.1 transport and Golgi organization 2 homolog [Momordica charantia] >XP_022153124.1 transport and Golgi organization 2 homolog [Momordica charantia] >XP_022153125.1 transport and Golgi organization 2 homolog [Momordica charantia] >XP_022153126.1 transport and Golgi organization 2 homolog [Momordica charantia])

HSP 1 Score: 417.5 bits (1072), Expect = 8.5e-113
Identity = 205/271 (75.65%), Postives = 223/271 (82.29%), Query Frame = 0

Query: 25  MCIAAFVWQ-------------------PTKPVSWWEDGEIAGGRDEVAGGTWLGCSRKG 84
           MCIAAF+WQ                   PT+P SWWEDGEIAGGRDEVAGGTWL CSRKG
Sbjct: 1   MCIAAFLWQSHPLYSLILLHNRDELLNRPTRPASWWEDGEIAGGRDEVAGGTWLACSRKG 60

Query: 85  RVAFVTNVLELHVLPEAKSRGNLPVLFLQSTKSPKEFAEEIKAEAYQYNGFNLILIDICS 144
           RV+FVTNVLELH LPEAKSRGNLP+LFLQS KSPKEFAEEI+AEAYQYNGFNL++ DI S
Sbjct: 61  RVSFVTNVLELHTLPEAKSRGNLPLLFLQSRKSPKEFAEEIRAEAYQYNGFNLVVADISS 120

Query: 145 KSMVYITNRSKGDHILIQEVPPGIHVLSNAELDSPWHKAERLRLKFKEQLRTFDEGDIPE 204
            +MVYI+NRS+GD I+IQEVPPG+HVLSNAELDSPWHKA+RLRLKF EQL   D G+IP 
Sbjct: 121 NTMVYISNRSEGDRIVIQEVPPGLHVLSNAELDSPWHKAQRLRLKFNEQLHKCDGGEIPV 180

Query: 205 KEMIERLMRDTTKADESKLPHIWSLDWEFNLSPVFVQFQTPM-----GSYGTRSTTVLTI 264
           KEMIERLMRDT KADESKLPHI SLDWE +LSP+FVQ QT       GS+GTRSTTVLTI
Sbjct: 181 KEMIERLMRDTVKADESKLPHICSLDWELDLSPIFVQIQTSQFFNIRGSFGTRSTTVLTI 240

Query: 265 STSGRAKFYETYLDKDTWKEKTLSYRIEQWN 272
           S +G  KFYETYLD DTWKEKTLSYRIEQWN
Sbjct: 241 SATGNVKFYETYLDNDTWKEKTLSYRIEQWN 271

BLAST of Spg025258 vs. NCBI nr
Match: XP_008462211.1 (PREDICTED: transport and Golgi organization 2 homolog [Cucumis melo] >XP_008462220.1 PREDICTED: transport and Golgi organization 2 homolog [Cucumis melo])

HSP 1 Score: 401.7 bits (1031), Expect = 4.9e-108
Identity = 195/263 (74.14%), Postives = 218/263 (82.89%), Query Frame = 0

Query: 25  MCIAAFVWQ-------------------PTKPVSWWEDGEIAGGRDEVAGGTWLGCSRKG 84
           MCIAAFVWQ                   PTKP+SWWED EIAGGRDEV GGTWLGCSRKG
Sbjct: 1   MCIAAFVWQSHSLYSLILFQNRDEYHNRPTKPMSWWEDHEIAGGRDEVGGGTWLGCSRKG 60

Query: 85  RVAFVTNVLELHVLPEAKSRGNLPVLFLQSTKSPKEFAEEIKAEAYQYNGFNLILIDICS 144
           +VAFVTNVLE H +PEAKSRGNLP+LFLQSTKSPKEFAEEIKAEAY+YNGFNL++ DI +
Sbjct: 61  KVAFVTNVLEPHEIPEAKSRGNLPLLFLQSTKSPKEFAEEIKAEAYRYNGFNLVVADIST 120

Query: 145 KSMVYITNRSKGDHILIQEVPPGIHVLSNAELDSPWHKAERLRLKFKEQLRTFDEGDIPE 204
           K+MVYI+NR+KGDHILIQEVPPG+HVLSNAELDS WHK +RLRLKF EQLR + EG+IPE
Sbjct: 121 KTMVYISNRTKGDHILIQEVPPGLHVLSNAELDSAWHKVQRLRLKFNEQLRIYGEGEIPE 180

Query: 205 KEMIERLMRDTTKADESKLPHIWSLDWEFNLSPVFVQFQTPMGSYGTRSTTVLTISTSGR 264
           K M+ERLMRD  KADESKLPHI S DWE+N S VFVQFQTP+G +GTRST+VLTI+ +G 
Sbjct: 181 KAMVERLMRDHVKADESKLPHISSPDWEYNTSSVFVQFQTPLGCFGTRSTSVLTITKTGM 240

Query: 265 AKFYETYLDKDTWKEKTLSYRIE 269
             FYETYL+  TWKEKTLSY IE
Sbjct: 241 VHFYETYLETGTWKEKTLSYFIE 263

BLAST of Spg025258 vs. NCBI nr
Match: XP_011654181.1 (transport and Golgi organization 2 homolog [Cucumis sativus] >XP_011654185.1 transport and Golgi organization 2 homolog [Cucumis sativus] >XP_031739931.1 transport and Golgi organization 2 homolog [Cucumis sativus] >XP_031739936.1 transport and Golgi organization 2 homolog [Cucumis sativus] >XP_031739942.1 transport and Golgi organization 2 homolog [Cucumis sativus] >XP_031739946.1 transport and Golgi organization 2 homolog [Cucumis sativus] >XP_031739952.1 transport and Golgi organization 2 homolog [Cucumis sativus] >XP_031739957.1 transport and Golgi organization 2 homolog [Cucumis sativus] >XP_031739963.1 transport and Golgi organization 2 homolog [Cucumis sativus] >XP_031739965.1 transport and Golgi organization 2 homolog [Cucumis sativus])

HSP 1 Score: 393.7 bits (1010), Expect = 1.3e-105
Identity = 191/263 (72.62%), Postives = 216/263 (82.13%), Query Frame = 0

Query: 25  MCIAAFVWQ-------------------PTKPVSWWEDGEIAGGRDEVAGGTWLGCSRKG 84
           MCIAAFVWQ                   PTKP+SWWED EIAGGRDEV GGTWLGCSRKG
Sbjct: 1   MCIAAFVWQSHSLYSLILFQNRDEYHNRPTKPMSWWEDNEIAGGRDEVGGGTWLGCSRKG 60

Query: 85  RVAFVTNVLELHVLPEAKSRGNLPVLFLQSTKSPKEFAEEIKAEAYQYNGFNLILIDICS 144
           +VAFVTNVLE H +P+AKSRG+LP+LFLQSTKSPKEFAEEIKAEAYQYNGFNLI+ DI +
Sbjct: 61  KVAFVTNVLEPHEIPDAKSRGHLPLLFLQSTKSPKEFAEEIKAEAYQYNGFNLIVADIST 120

Query: 145 KSMVYITNRSKGDHILIQEVPPGIHVLSNAELDSPWHKAERLRLKFKEQLRTFDEGDIPE 204
           K+M+YI+NR+KGDHILIQEVPPG+HVLSNAELD+ WHK +RLRLKF EQL  + EG+IPE
Sbjct: 121 KTMIYISNRTKGDHILIQEVPPGLHVLSNAELDTAWHKVQRLRLKFNEQLCIYAEGEIPE 180

Query: 205 KEMIERLMRDTTKADESKLPHIWSLDWEFNLSPVFVQFQTPMGSYGTRSTTVLTISTSGR 264
           KEM+ERLMRD  KADESKLP I S DWE+N S VFVQFQTP+G +GTRST+VLTI+ +G 
Sbjct: 181 KEMVERLMRDHVKADESKLPRISSPDWEYNTSSVFVQFQTPLGCFGTRSTSVLTITKTGM 240

Query: 265 AKFYETYLDKDTWKEKTLSYRIE 269
             FYETYL+  TWKEK LSY IE
Sbjct: 241 VHFYETYLETGTWKEKALSYFIE 263

BLAST of Spg025258 vs. NCBI nr
Match: KAA0052070.1 (transport and Golgi organization 2-like protein [Cucumis melo var. makuwa] >TYK00685.1 transport and Golgi organization 2-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 381.3 bits (978), Expect = 6.8e-102
Identity = 185/254 (72.83%), Postives = 209/254 (82.28%), Query Frame = 0

Query: 25  MCIAAFVWQ-------------------PTKPVSWWEDGEIAGGRDEVAGGTWLGCSRKG 84
           MCIAAFVWQ                   PTKP+SWWED EIAGGRDEV GGTWLGCSRKG
Sbjct: 1   MCIAAFVWQSHSLYSLILFQNRDEYHNRPTKPMSWWEDHEIAGGRDEVGGGTWLGCSRKG 60

Query: 85  RVAFVTNVLELHVLPEAKSRGNLPVLFLQSTKSPKEFAEEIKAEAYQYNGFNLILIDICS 144
           +VAFVTNVLE H +PEAKSRGNLP+LFLQSTKSPKEFAEEIKAEAY+YNGFNL++ DI +
Sbjct: 61  KVAFVTNVLEPHEIPEAKSRGNLPLLFLQSTKSPKEFAEEIKAEAYRYNGFNLVVADIST 120

Query: 145 KSMVYITNRSKGDHILIQEVPPGIHVLSNAELDSPWHKAERLRLKFKEQLRTFDEGDIPE 204
           K+MVYI+NR+KGDHILIQEVPPG+HVLSNAELDS WHK +RLRLKF EQLR + EG+IPE
Sbjct: 121 KTMVYISNRTKGDHILIQEVPPGLHVLSNAELDSAWHKVQRLRLKFNEQLRIYGEGEIPE 180

Query: 205 KEMIERLMRDTTKADESKLPHIWSLDWEFNLSPVFVQFQTPMGSYGTRSTTVLTISTSGR 260
           K M+ERLMRD  KADESKLPHI S DWE+N S VFVQFQTP+G +GTRST+VLTI+ +G 
Sbjct: 181 KAMVERLMRDHVKADESKLPHISSPDWEYNTSSVFVQFQTPLGCFGTRSTSVLTITKTGM 240

BLAST of Spg025258 vs. NCBI nr
Match: XP_018809036.1 (transport and Golgi organization 2 homolog [Juglans regia] >KAF5462240.1 hypothetical protein F2P56_018263 [Juglans regia])

HSP 1 Score: 368.2 bits (944), Expect = 5.9e-98
Identity = 176/264 (66.67%), Postives = 207/264 (78.41%), Query Frame = 0

Query: 25  MCIAAFVWQ-------------------PTKPVSWWEDGEIAGGRDEVAGGTWLGCSRKG 84
           MCIAAF WQ                   PTK V+WWE G+I GGRDEVAGGTWL CSR G
Sbjct: 1   MCIAAFAWQVHPLFPFLVFQNRDEYHNRPTKQVAWWEGGDILGGRDEVAGGTWLACSRGG 60

Query: 85  RVAFVTNVLELHVLPEAKSRGNLPVLFLQSTKSPKEFAEEIKAEAYQYNGFNLILIDICS 144
           RVAF+TNVLELH+LPEAKSRG+LPVLFL+STK+PKEFAEE+K EA+QYNGFNLI+ DI S
Sbjct: 61  RVAFLTNVLELHILPEAKSRGDLPVLFLESTKTPKEFAEELKTEAHQYNGFNLIVADISS 120

Query: 145 KSMVYITNRSKGDHILIQEVPPGIHVLSNAELDSPWHKAERLRLKFKEQLRTFDEGDIPE 204
           KSMVY++NR KG+ I IQEV PGIHVLSNA+LDSPWHKA+RL   FKEQL  + +G++P 
Sbjct: 121 KSMVYVSNRPKGEPIAIQEVSPGIHVLSNAKLDSPWHKAQRLGPSFKEQLCKYGKGEVPV 180

Query: 205 KEMIERLMRDTTKADESKLPHIWSLDWEFNLSPVFVQFQTPMGSYGTRSTTVLTISTSGR 264
           K+M++ LMRDT KADESKLPHI SLDWEFNLS +FV+  TP+G YGTRST  LT+   G 
Sbjct: 181 KDMLKMLMRDTVKADESKLPHICSLDWEFNLSSIFVEVDTPLGPYGTRSTAALTVKACGE 240

Query: 265 AKFYETYLDKDTWKEKTLSYRIEQ 270
             F+E +LDK+TWKEKT+SY I +
Sbjct: 241 VSFFEMFLDKNTWKEKTVSYYIRR 264

BLAST of Spg025258 vs. ExPASy Swiss-Prot
Match: P54797 (Transport and Golgi organization 2 homolog OS=Mus musculus OX=10090 GN=Tango2 PE=1 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 3.8e-23
Identity = 78/256 (30.47%), Postives = 131/256 (51.17%), Query Frame = 0

Query: 30  FVWQPTKPVSWW-EDGEIAGGRD---EVAGGTWLGCSRKGRVAFVTNVLELHVLPEAKSR 89
           F  +P+K   +W  + EI  G D     AGGTWLG S +G++  +TN L+    P+A+ R
Sbjct: 29  FYNRPSKLADFWGNNSEILSGLDMEEGKAGGTWLGISTRGKLGALTNYLQPRQEPDARGR 88

Query: 90  GNLPVLFLQSTKSPKEFAEEIKAEAYQYNGFNLILIDICSKS---MVYITNRSKGDHILI 149
           G L   FL S      + +++  E + YNGFN+I  D+ +     + Y  NR + + I++
Sbjct: 89  GELVSHFLTSDMDSLSYLKKVSTEGHLYNGFNIIAADLSTSKGDVVCYYGNRGEPEPIVL 148

Query: 150 QEVPPGIHVLSNAELDSPWHKAERLRLKFKEQL--RTFDEGDIPEKEMIERLMRDTTKAD 209
               PG + LSNA L++PW K     L F +QL     ++ +   K+++   + D    +
Sbjct: 149 ---TPGTYGLSNALLETPWKK-----LCFGKQLFMEAVEQSEALPKDVLVTQLLDVLNNE 208

Query: 210 ESKLPH--IWSLDWEFNLSPVFVQFQT---PMGSYGTRSTTVLTISTSGRAKFYE-TYLD 269
           E++LP   I     E+ + P+  ++        SYGTR+ T++ +  +G   F E + LD
Sbjct: 209 EAQLPDPAIEDQGQEY-VQPILNKYAAVWCRCASYGTRTNTIILVDANGHVTFTERSMLD 268

BLAST of Spg025258 vs. ExPASy Swiss-Prot
Match: Q29RZ5 (Transport and Golgi organization protein 2 homolog OS=Bos taurus OX=9913 GN=TANGO2 PE=2 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 6.1e-21
Identity = 75/257 (29.18%), Postives = 129/257 (50.19%), Query Frame = 0

Query: 30  FVWQPTKPVSWW-EDGEIAGGRD---EVAGGTWLGCSRKGRVAFVTNVLELHVLPEAKSR 89
           F  +P +   +W  + E+  G D      GGTWLG S +G++A +TN L+  +   A+ R
Sbjct: 29  FYHRPARAADFWGSNNEVLSGLDMEEGKEGGTWLGISTRGKLAALTNYLQPRLNRNARGR 88

Query: 90  GNLPVLFLQSTKSPKEFAEEIKAEAYQYNGFNLILIDICSKS---MVYITNRSKGDHILI 149
           G L   FL S      + +++ AE + YNGFNLI  D+ ++    + Y  NR + + +++
Sbjct: 89  GELVAQFLTSDMDSLSYLKKVSAEGHLYNGFNLIAADLSAEKGDVICYYGNRGEREPVVL 148

Query: 150 QEVPPGIHVLSNAELDSPWHKAERLRLKFKEQ--LRTFDEG-DIPEKEMIERLMRDTTKA 209
               PG + LSNA L++PW K     L F +Q  L   + G ++P   ++ +L+      
Sbjct: 149 ---APGTYGLSNALLETPWRK-----LCFGKQLFLEAVERGRELPRDALVAQLLA-VLSN 208

Query: 210 DESKLPH--IWSLDWEFNLSPVFVQFQ---TPMGSYGTRSTTVLTISTSGRAKFYETYL- 269
           DE++LP   I +   E+ + P+  ++         YGTR++TV+ +   G   F E  + 
Sbjct: 209 DEAQLPDPAIEAQGREY-VRPILSKYAAVCVRCPDYGTRTSTVILVDADGHVTFTERSML 268

BLAST of Spg025258 vs. ExPASy Swiss-Prot
Match: Q6ICL3 (Transport and Golgi organization protein 2 homolog OS=Homo sapiens OX=9606 GN=TANGO2 PE=2 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 3.0e-20
Identity = 75/256 (29.30%), Postives = 127/256 (49.61%), Query Frame = 0

Query: 30  FVWQPTKPVSWW-EDGEIAGGRD---EVAGGTWLGCSRKGRVAFVTNVLELHVLPEAKSR 89
           F  +P+K   +W  + EI  G D      GGTWLG S +G++A +TN L+  +  +A+ R
Sbjct: 29  FYSRPSKLADFWGNNNEILSGLDMEEGKEGGTWLGISTRGKLAALTNYLQPQLDWQARGR 88

Query: 90  GNLPVLFLQSTKSPKEFAEEIKAEAYQYNGFNLILIDICSKS---MVYITNRSKGDHILI 149
           G L   FL +      + +++  E + YNGFNLI  D+ +     + Y  NR + D I++
Sbjct: 89  GELVTHFLTTDVDSLSYLKKVSMEGHLYNGFNLIAADLSTAKGDVICYYGNRGEPDPIVL 148

Query: 150 QEVPPGIHVLSNAELDSPWHKAERLRLKFKEQ--LRTFDEGDIPEKEMIERLMRDTTKAD 209
               PG + LSNA L++PW K     L F +Q  L   +      K+++   + D    +
Sbjct: 149 ---TPGTYGLSNALLETPWRK-----LCFGKQLFLEAVERSQALPKDVLIASLLDVLNNE 208

Query: 210 ESKLPH--IWSLDWEFNLSPVFVQFQ---TPMGSYGTRSTTVLTISTSGRAKFYE-TYLD 269
           E++LP   I     E+ + P+  ++         YGTR+ T++ +   G   F E + +D
Sbjct: 209 EAQLPDPAIEDQGGEY-VQPMLSKYAAVCVRCPGYGTRTNTIILVDADGHVTFTERSMMD 268

BLAST of Spg025258 vs. ExPASy TrEMBL
Match: A0A6J1DFX0 (transport and Golgi organization 2 homolog OS=Momordica charantia OX=3673 GN=LOC111020699 PE=4 SV=1)

HSP 1 Score: 417.5 bits (1072), Expect = 4.1e-113
Identity = 205/271 (75.65%), Postives = 223/271 (82.29%), Query Frame = 0

Query: 25  MCIAAFVWQ-------------------PTKPVSWWEDGEIAGGRDEVAGGTWLGCSRKG 84
           MCIAAF+WQ                   PT+P SWWEDGEIAGGRDEVAGGTWL CSRKG
Sbjct: 1   MCIAAFLWQSHPLYSLILLHNRDELLNRPTRPASWWEDGEIAGGRDEVAGGTWLACSRKG 60

Query: 85  RVAFVTNVLELHVLPEAKSRGNLPVLFLQSTKSPKEFAEEIKAEAYQYNGFNLILIDICS 144
           RV+FVTNVLELH LPEAKSRGNLP+LFLQS KSPKEFAEEI+AEAYQYNGFNL++ DI S
Sbjct: 61  RVSFVTNVLELHTLPEAKSRGNLPLLFLQSRKSPKEFAEEIRAEAYQYNGFNLVVADISS 120

Query: 145 KSMVYITNRSKGDHILIQEVPPGIHVLSNAELDSPWHKAERLRLKFKEQLRTFDEGDIPE 204
            +MVYI+NRS+GD I+IQEVPPG+HVLSNAELDSPWHKA+RLRLKF EQL   D G+IP 
Sbjct: 121 NTMVYISNRSEGDRIVIQEVPPGLHVLSNAELDSPWHKAQRLRLKFNEQLHKCDGGEIPV 180

Query: 205 KEMIERLMRDTTKADESKLPHIWSLDWEFNLSPVFVQFQTPM-----GSYGTRSTTVLTI 264
           KEMIERLMRDT KADESKLPHI SLDWE +LSP+FVQ QT       GS+GTRSTTVLTI
Sbjct: 181 KEMIERLMRDTVKADESKLPHICSLDWELDLSPIFVQIQTSQFFNIRGSFGTRSTTVLTI 240

Query: 265 STSGRAKFYETYLDKDTWKEKTLSYRIEQWN 272
           S +G  KFYETYLD DTWKEKTLSYRIEQWN
Sbjct: 241 SATGNVKFYETYLDNDTWKEKTLSYRIEQWN 271

BLAST of Spg025258 vs. ExPASy TrEMBL
Match: A0A1S3CGF4 (transport and Golgi organization 2 homolog OS=Cucumis melo OX=3656 GN=LOC103500621 PE=4 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 2.4e-108
Identity = 195/263 (74.14%), Postives = 218/263 (82.89%), Query Frame = 0

Query: 25  MCIAAFVWQ-------------------PTKPVSWWEDGEIAGGRDEVAGGTWLGCSRKG 84
           MCIAAFVWQ                   PTKP+SWWED EIAGGRDEV GGTWLGCSRKG
Sbjct: 1   MCIAAFVWQSHSLYSLILFQNRDEYHNRPTKPMSWWEDHEIAGGRDEVGGGTWLGCSRKG 60

Query: 85  RVAFVTNVLELHVLPEAKSRGNLPVLFLQSTKSPKEFAEEIKAEAYQYNGFNLILIDICS 144
           +VAFVTNVLE H +PEAKSRGNLP+LFLQSTKSPKEFAEEIKAEAY+YNGFNL++ DI +
Sbjct: 61  KVAFVTNVLEPHEIPEAKSRGNLPLLFLQSTKSPKEFAEEIKAEAYRYNGFNLVVADIST 120

Query: 145 KSMVYITNRSKGDHILIQEVPPGIHVLSNAELDSPWHKAERLRLKFKEQLRTFDEGDIPE 204
           K+MVYI+NR+KGDHILIQEVPPG+HVLSNAELDS WHK +RLRLKF EQLR + EG+IPE
Sbjct: 121 KTMVYISNRTKGDHILIQEVPPGLHVLSNAELDSAWHKVQRLRLKFNEQLRIYGEGEIPE 180

Query: 205 KEMIERLMRDTTKADESKLPHIWSLDWEFNLSPVFVQFQTPMGSYGTRSTTVLTISTSGR 264
           K M+ERLMRD  KADESKLPHI S DWE+N S VFVQFQTP+G +GTRST+VLTI+ +G 
Sbjct: 181 KAMVERLMRDHVKADESKLPHISSPDWEYNTSSVFVQFQTPLGCFGTRSTSVLTITKTGM 240

Query: 265 AKFYETYLDKDTWKEKTLSYRIE 269
             FYETYL+  TWKEKTLSY IE
Sbjct: 241 VHFYETYLETGTWKEKTLSYFIE 263

BLAST of Spg025258 vs. ExPASy TrEMBL
Match: A0A0A0LSJ0 (Sterile domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G144330 PE=4 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 3.4e-107
Identity = 193/265 (72.83%), Postives = 218/265 (82.26%), Query Frame = 0

Query: 23  GTMCIAAFVWQ-------------------PTKPVSWWEDGEIAGGRDEVAGGTWLGCSR 82
           GTMCIAAFVWQ                   PTKP+SWWED EIAGGRDEV GGTWLGCSR
Sbjct: 137 GTMCIAAFVWQSHSLYSLILFQNRDEYHNRPTKPMSWWEDNEIAGGRDEVGGGTWLGCSR 196

Query: 83  KGRVAFVTNVLELHVLPEAKSRGNLPVLFLQSTKSPKEFAEEIKAEAYQYNGFNLILIDI 142
           KG+VAFVTNVLE H +P+AKSRG+LP+LFLQSTKSPKEFAEEIKAEAYQYNGFNLI+ DI
Sbjct: 197 KGKVAFVTNVLEPHEIPDAKSRGHLPLLFLQSTKSPKEFAEEIKAEAYQYNGFNLIVADI 256

Query: 143 CSKSMVYITNRSKGDHILIQEVPPGIHVLSNAELDSPWHKAERLRLKFKEQLRTFDEGDI 202
            +K+M+YI+NR+KGDHILIQEVPPG+HVLSNAELD+ WHK +RLRLKF EQL  + EG+I
Sbjct: 257 STKTMIYISNRTKGDHILIQEVPPGLHVLSNAELDTAWHKVQRLRLKFNEQLCIYAEGEI 316

Query: 203 PEKEMIERLMRDTTKADESKLPHIWSLDWEFNLSPVFVQFQTPMGSYGTRSTTVLTISTS 262
           PEKEM+ERLMRD  KADESKLP I S DWE+N S VFVQFQTP+G +GTRST+VLTI+ +
Sbjct: 317 PEKEMVERLMRDHVKADESKLPRISSPDWEYNTSSVFVQFQTPLGCFGTRSTSVLTITKT 376

Query: 263 GRAKFYETYLDKDTWKEKTLSYRIE 269
           G   FYETYL+  TWKEK LSY IE
Sbjct: 377 GMVHFYETYLETGTWKEKALSYFIE 401

BLAST of Spg025258 vs. ExPASy TrEMBL
Match: A0A5A7UA61 (Transport and Golgi organization 2-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1371G00250 PE=4 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 3.3e-102
Identity = 185/254 (72.83%), Postives = 209/254 (82.28%), Query Frame = 0

Query: 25  MCIAAFVWQ-------------------PTKPVSWWEDGEIAGGRDEVAGGTWLGCSRKG 84
           MCIAAFVWQ                   PTKP+SWWED EIAGGRDEV GGTWLGCSRKG
Sbjct: 1   MCIAAFVWQSHSLYSLILFQNRDEYHNRPTKPMSWWEDHEIAGGRDEVGGGTWLGCSRKG 60

Query: 85  RVAFVTNVLELHVLPEAKSRGNLPVLFLQSTKSPKEFAEEIKAEAYQYNGFNLILIDICS 144
           +VAFVTNVLE H +PEAKSRGNLP+LFLQSTKSPKEFAEEIKAEAY+YNGFNL++ DI +
Sbjct: 61  KVAFVTNVLEPHEIPEAKSRGNLPLLFLQSTKSPKEFAEEIKAEAYRYNGFNLVVADIST 120

Query: 145 KSMVYITNRSKGDHILIQEVPPGIHVLSNAELDSPWHKAERLRLKFKEQLRTFDEGDIPE 204
           K+MVYI+NR+KGDHILIQEVPPG+HVLSNAELDS WHK +RLRLKF EQLR + EG+IPE
Sbjct: 121 KTMVYISNRTKGDHILIQEVPPGLHVLSNAELDSAWHKVQRLRLKFNEQLRIYGEGEIPE 180

Query: 205 KEMIERLMRDTTKADESKLPHIWSLDWEFNLSPVFVQFQTPMGSYGTRSTTVLTISTSGR 260
           K M+ERLMRD  KADESKLPHI S DWE+N S VFVQFQTP+G +GTRST+VLTI+ +G 
Sbjct: 181 KAMVERLMRDHVKADESKLPHISSPDWEYNTSSVFVQFQTPLGCFGTRSTSVLTITKTGM 240

BLAST of Spg025258 vs. ExPASy TrEMBL
Match: A0A2I4DPG2 (transport and Golgi organization 2 homolog OS=Juglans regia OX=51240 GN=LOC108982189 PE=4 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 2.9e-98
Identity = 176/264 (66.67%), Postives = 207/264 (78.41%), Query Frame = 0

Query: 25  MCIAAFVWQ-------------------PTKPVSWWEDGEIAGGRDEVAGGTWLGCSRKG 84
           MCIAAF WQ                   PTK V+WWE G+I GGRDEVAGGTWL CSR G
Sbjct: 1   MCIAAFAWQVHPLFPFLVFQNRDEYHNRPTKQVAWWEGGDILGGRDEVAGGTWLACSRGG 60

Query: 85  RVAFVTNVLELHVLPEAKSRGNLPVLFLQSTKSPKEFAEEIKAEAYQYNGFNLILIDICS 144
           RVAF+TNVLELH+LPEAKSRG+LPVLFL+STK+PKEFAEE+K EA+QYNGFNLI+ DI S
Sbjct: 61  RVAFLTNVLELHILPEAKSRGDLPVLFLESTKTPKEFAEELKTEAHQYNGFNLIVADISS 120

Query: 145 KSMVYITNRSKGDHILIQEVPPGIHVLSNAELDSPWHKAERLRLKFKEQLRTFDEGDIPE 204
           KSMVY++NR KG+ I IQEV PGIHVLSNA+LDSPWHKA+RL   FKEQL  + +G++P 
Sbjct: 121 KSMVYVSNRPKGEPIAIQEVSPGIHVLSNAKLDSPWHKAQRLGPSFKEQLCKYGKGEVPV 180

Query: 205 KEMIERLMRDTTKADESKLPHIWSLDWEFNLSPVFVQFQTPMGSYGTRSTTVLTISTSGR 264
           K+M++ LMRDT KADESKLPHI SLDWEFNLS +FV+  TP+G YGTRST  LT+   G 
Sbjct: 181 KDMLKMLMRDTVKADESKLPHICSLDWEFNLSSIFVEVDTPLGPYGTRSTAALTVKACGE 240

Query: 265 AKFYETYLDKDTWKEKTLSYRIEQ 270
             F+E +LDK+TWKEKT+SY I +
Sbjct: 241 VSFFEMFLDKNTWKEKTVSYYIRR 264

BLAST of Spg025258 vs. TAIR 10
Match: AT4G38260.1 (Protein of unknown function (DUF833) )

HSP 1 Score: 251.1 bits (640), Expect = 9.9e-67
Identity = 129/271 (47.60%), Postives = 170/271 (62.73%), Query Frame = 0

Query: 25  MCIAAFVWQ-------------------PTKPVSWWEDGEIAGGRDEVAGGTWLGCSRKG 84
           MCIA F+WQ                    T+ + WWEDGE  GGRD V GGTWLGC+R G
Sbjct: 1   MCIAVFLWQSHPLYPFLLFLNRDEDHNRATEALRWWEDGETVGGRDLVGGGTWLGCTRHG 60

Query: 85  RVAFVTNVLELHVLPEAKSRGNLPVLFLQSTKSPKEFAEEIKAEAYQYNGFNLILIDICS 144
           R+AF+TN  E    P AKSRG+LP+ +LQS KSP EFAEEI+ E   YNGFNL++  + S
Sbjct: 61  RLAFLTNFKEASSFPAAKSRGDLPLRYLQSEKSPAEFAEEIQDEISLYNGFNLVVAHVLS 120

Query: 145 KSMVYITNRSKGDHILIQEVPPGIHVLSNAELDSPWHKAERLRLKFKEQLRTFDEGDIPE 204
           KSM+YITNR      L+ +V PGIHVLSNA LDSPW K  RLR  F++ L     G+ P 
Sbjct: 121 KSMIYITNRPPHGDKLVTQVSPGIHVLSNANLDSPWPKCLRLREGFQQLLAENGSGEFPV 180

Query: 205 KEMIERLMRDTTKADESKLPHIWSLDWEFNLSPVFVQFQTPMGSYGTRSTTVLTIST--- 264
           K M+E +M +T K +E++LPH+++ + E++LS +FV  Q P G YGTRS + + + +   
Sbjct: 181 KTMVEEVMTNTVKDEETELPHVFTPETEYHLSSIFVDMQRPTGRYGTRSISAIIVKSHGD 240

Query: 265 ---SGRAKFYETYLDK-DTWKEKTLSYRIEQ 270
               G   FYE +L++ D+WKE T  + I Q
Sbjct: 241 GGGDGEICFYERHLEEGDSWKEHTQQFVIIQ 271

BLAST of Spg025258 vs. TAIR 10
Match: AT1G20740.1 (Protein of unknown function (DUF833) )

HSP 1 Score: 94.7 bits (234), Expect = 1.2e-19
Identity = 76/237 (32.07%), Postives = 116/237 (48.95%), Query Frame = 0

Query: 42  EDGEIAGGRDEVAGGTWLGCSRKGRVAFVTNVLELHVLPEAKSRG--NLPVLFLQSTKSP 101
           E  +I  GR     GTWLG S +GRVAF   ++E   +   +  G  +  + FL+S +SP
Sbjct: 46  ETDQILSGRCPETDGTWLGISTRGRVAF---LVEAGTINRDRFNGAESRTLEFLESNESP 105

Query: 102 KEFAEEIKAEAYQYN---GFNLILIDICSKSMVYITNRSKGDH--ILIQEVPPGIHVLSN 161
           ++FA+   A+  +      F+LI+ DI S SM+YI+     D+  +  + V PG+H LS+
Sbjct: 106 EDFAKSSAADYIRNKNTAAFHLIVADIASNSMLYISKPRFSDYGIVYTEPVGPGVHTLSS 165

Query: 162 AELDSP-WHKAERLRLKFKEQLRTFDEGDIPEKEMIERLMRDTTKADESKLPHIWSLDWE 221
           A LDS   ++  R+R  F E +   +   +P    I  +M D  KA ES L         
Sbjct: 166 AGLDSDVGYRDLRMRHSFCEMI---NRERLPPIRDIAEIMYDPVKAYESVL--------- 225

Query: 222 FNLSPV-FVQFQTPMGSYGTRSTTVLTISTSGRAKFYETYLD--KDTWKEKTLSYRI 268
             LS + FV  +     YGTR TT L +  +    F+E Y +   D W +   ++ I
Sbjct: 226 --LSSIFFVDMKIGYEHYGTRITTALVVKRTKEVLFFERYREIFNDDWDDHDFAFTI 265

BLAST of Spg025258 vs. TAIR 10
Match: AT1G20680.2 (Protein of unknown function (DUF833) )

HSP 1 Score: 92.4 bits (228), Expect = 5.9e-19
Identity = 73/241 (30.29%), Postives = 121/241 (50.21%), Query Frame = 0

Query: 39  SWWEDGEIAGGRDEVAGGTWLGCSRKGRVAFVTNVLELHVLPEAKSRGNL-PVLFLQSTK 98
           SW  +G+I  GR +   GTW G ++ GRVAF+ N   L    ++ S   L PV FL+   
Sbjct: 34  SWSRNGQILSGRCKANNGTWFGITKGGRVAFLVNTSLLLDRVKSYSGSELYPVRFLEGNM 93

Query: 99  SPKEFAEEIKA---EAYQYNGFNLILIDICSKSMVYITNRS--KGDHILIQEVPPGIHVL 158
           SP++FA E+K    E  + + ++L++ D+ S SMV+I   S  K D ++I+ VP G+H L
Sbjct: 94  SPEQFANEVKVHEKETNERHAYSLVVADMTSSSMVHILKPSDTKSD-VVIETVPFGVHTL 153

Query: 159 SNAE-LDSPWHKAERLRLKFKEQLRTFDEGDIPEKEMIE---RLMRDTTKADESKLPHIW 218
           S+ E LDS     + L  +   Q+   + G++ +++M E   R M D     ++   H  
Sbjct: 154 SSYEGLDSTDSARDLLLRRLFTQM-VGNLGNVQQRQMEEIAGRFMYDAQAGRDAVFYH-- 213

Query: 219 SLDWEFNLSPVFVQFQTPMGSYGTRSTTVLTISTSGRAKFYETYLDKD-TWKEKTLSYRI 269
           S D   N        +     +GT STT L +  +     +E Y++++  W     ++ I
Sbjct: 214 SRDEHPN-------GKLGTQRFGTTSTTALVVKRTREVMLFEKYMEQNGAWNTNNFAFNI 263

BLAST of Spg025258 vs. TAIR 10
Match: AT1G20730.1 (Protein of unknown function (DUF833) )

HSP 1 Score: 89.4 bits (220), Expect = 5.0e-18
Identity = 73/237 (30.80%), Postives = 115/237 (48.52%), Query Frame = 0

Query: 41  WEDGEIAGGRDEVAGGTWLGCSRKGRVAFVTNV--LELHVLPEAKSRGNLPVLFLQSTKS 100
           W++ ++  GR     GTWLG S +GRVAF+     +    +  A+ R    + FL+S  S
Sbjct: 36  WDESQVLSGRCAETDGTWLGISIRGRVAFLVEAGPVNRDRIIGAECR---TLDFLKSNLS 95

Query: 101 PKEFAEEIKAEAYQYNG--FNLILIDICSKSMVYI--TNRSKGDHILIQEVPPGIHVLSN 160
           P++FA+ +  ++ +  G  ++LI+ DI S SM YI   + S+   +  + V PG+H LS+
Sbjct: 96  PEDFADSLALDSGRNTGIAYHLIVADIVSNSMFYIYKPSLSEDGMVYTEPVGPGVHTLSS 155

Query: 161 AELDSP-WHKAERLRLKFKEQLRTFDEGDIPEKEMIERLMRDTTKADESKLPHIWSLDWE 220
           A LDS   H+  RL+  F E++    E   P   + E +M DT +A              
Sbjct: 156 AGLDSEVGHRDLRLKRYFSERINR--ELPEPISGLAEEVMYDTVEAINGD---------- 215

Query: 221 FNLSPVF-VQFQTPMGSYGTRSTTVLTISTSGRAKFYETYLDK--DTWKEKTLSYRI 268
             LS +F V        YGTR TT L +  + + +F+E Y  +  D W      + I
Sbjct: 216 -PLSSIFVVDTLIENEHYGTRCTTALVVRRTMQVRFFERYRARFNDDWNVHDFRFTI 256

BLAST of Spg025258 vs. TAIR 10
Match: AT1G20680.1 (Protein of unknown function (DUF833) )

HSP 1 Score: 82.4 bits (202), Expect = 6.1e-16
Identity = 70/240 (29.17%), Postives = 116/240 (48.33%), Query Frame = 0

Query: 39  SWWEDGEIAGGRDEVAGGTWLGCSRKGRVAFVTNVLELHVLPEAKSRGNLPVLFLQSTKS 98
           SW  +G+I  GR +   GTW G ++ GRVAF+ N   L  L   KS     +       S
Sbjct: 42  SWSRNGQILSGRCKANNGTWFGITKGGRVAFLVNTSLL--LDRVKSYSGSEL--YPGNMS 101

Query: 99  PKEFAEEIKA---EAYQYNGFNLILIDICSKSMVYITNRS--KGDHILIQEVPPGIHVLS 158
           P++FA E+K    E  + + ++L++ D+ S SMV+I   S  K D ++I+ VP G+H LS
Sbjct: 102 PEQFANEVKVHEKETNERHAYSLVVADMTSSSMVHILKPSDTKSD-VVIETVPFGVHTLS 161

Query: 159 NAE-LDSPWHKAERLRLKFKEQLRTFDEGDIPEKEMIE---RLMRDTTKADESKLPHIWS 218
           + E LDS     + L  +   Q+   + G++ +++M E   R M D     ++   H  S
Sbjct: 162 SYEGLDSTDSARDLLLRRLFTQM-VGNLGNVQQRQMEEIAGRFMYDAQAGRDAVFYH--S 221

Query: 219 LDWEFNLSPVFVQFQTPMGSYGTRSTTVLTISTSGRAKFYETYLDKD-TWKEKTLSYRIE 269
            D   N        +     +GT STT L +  +     +E Y++++  W     ++ I+
Sbjct: 222 RDEHPN-------GKLGTQRFGTTSTTALVVKRTREVMLFEKYMEQNGAWNTNNFAFNIQ 266