Spg025222 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg025222
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	RING-type E3 ubiquitin transferase
Location	scaffold13: 40360378 .. 40361736 (+)
RNA-Seq Expression	Spg025222
Synteny	Spg025222

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCGATGTACCAGCCCTCTACTTGGAGACAAGGGGATCCCAAGCTCGTTGTGGGTTGCGGAGCGCAGATTCTAGATCTGGAAACTGTCGTCAAAGATGGGATTCTCGGCGGCGGCGGCGGCGGTGGGCTCATCTCCGGCGGTGGTGCTGAGAAATTGGATCTGAAGGAGATGATTGAGGAGCTGGAATCATCGATAGAGGTCCCATCGGTGTTCATTTGCCCAATTTCGCTCGAGCCGATGCAAGATCCAGTGACCCTTTGCTCCGGGCAGACCTACGAGCGGTCCAACATTCTGAAATGGTTTTCTTTAGGCCATTTAACTTGCCCCACAACAATGCAAGAGCTTTGGGACGATTCCGTCACACCGAATCGGACCCTTCACCAGTTGATTCACACCTGGTTTTTGCAGAAGTACTTGGCATTGAAGAAGAGATCAGAGGACGTTCAGGGCAGAGTTTTAGAGCTTTTGGATTCGTTGAAGAAGGTCAAGGGCCAAGCTAGGGTTCAGTCCCTTAAAGATCTCCGACATGTCGTCGCCACCCATTCCTCCGCCAAGAAAATGGTGGTCGAAAACGGCGGCGTCGAGCTAATCTCCTCTCTTTTGGGTTCTTTCACTTCTCACGCAGTTGGGTCCGAAGCCGTTGGGATCCTCGTCAATTTGGATCTCGATTCGGAGTTGAAGAAGAATCTGATTCAACCCACGAGAATGTCCCTAATTGCAGATATTTTAAACGAAGGGTCCATCGAAACCAAGATCAATTGCACGAAATTCATCAAGATGTTGATTGAGGGCGACGATTTAAGAACAGAGAACGTTTCAAGTTTGAGTCTACTCGTCGGATTGTTGAGAATGGTGAAGGATCAGAGACACCCAAATGGAGTAACCGCCGGACTCAGTTTACTCCGAACGCTCTGTTCAAACGAATCAATCAGAAAGCAACTCGTAAGCATCGGCGCCGTCGCGAAACTCGGCGGCGCCCTCTCCGGTTTAAACGTCGATTGCTTGGAATCGGCGCTTTATATTCTCGATGTTTTGTCGTCCCTTCCAGAGGGGAGATTAGCGTTGAAAGATTGCGAAGATACGATTAGGAATTTGGTGAAATTGTTGATGAAAGTATCTGAGATTTGTACTCAATTTGCGTTGTCGATTTTGTGGGCGGTTTTCAAGCTAGCGCCGGAGGAATGTGCGGTGCTCGCGGTGGAGGCGGGATTGGCGGCGAAGCTGCTGCTTGTGATACAAAGTGGGTGCAATAAAGAGCTAAAACAGAGATCTTCTGAGCTATTGAAATTGTGTAGTCTAAATTACCCAGATTCTATCTTCATTTCTAAATGTAAGCTTACGAGAACTATACAATAA

mRNA sequence

ATGCCGATGTACCAGCCCTCTACTTGGAGACAAGGGGATCCCAAGCTCGTTGTGGGTTGCGGAGCGCAGATTCTAGATCTGGAAACTGTCGTCAAAGATGGGATTCTCGGCGGCGGCGGCGGCGGTGGGCTCATCTCCGGCGGTGGTGCTGAGAAATTGGATCTGAAGGAGATGATTGAGGAGCTGGAATCATCGATAGAGGTCCCATCGGTGTTCATTTGCCCAATTTCGCTCGAGCCGATGCAAGATCCAGTGACCCTTTGCTCCGGGCAGACCTACGAGCGGTCCAACATTCTGAAATGGTTTTCTTTAGGCCATTTAACTTGCCCCACAACAATGCAAGAGCTTTGGGACGATTCCGTCACACCGAATCGGACCCTTCACCAGTTGATTCACACCTGGTTTTTGCAGAAGTACTTGGCATTGAAGAAGAGATCAGAGGACGTTCAGGGCAGAGTTTTAGAGCTTTTGGATTCGTTGAAGAAGGTCAAGGGCCAAGCTAGGGTTCAGTCCCTTAAAGATCTCCGACATGTCGTCGCCACCCATTCCTCCGCCAAGAAAATGGTGGTCGAAAACGGCGGCGTCGAGCTAATCTCCTCTCTTTTGGGTTCTTTCACTTCTCACGCAGTTGGGTCCGAAGCCGTTGGGATCCTCGTCAATTTGGATCTCGATTCGGAGTTGAAGAAGAATCTGATTCAACCCACGAGAATGTCCCTAATTGCAGATATTTTAAACGAAGGGTCCATCGAAACCAAGATCAATTGCACGAAATTCATCAAGATGTTGATTGAGGGCGACGATTTAAGAACAGAGAACGTTTCAAGTTTGAGTCTACTCGTCGGATTGTTGAGAATGGTGAAGGATCAGAGACACCCAAATGGAGTAACCGCCGGACTCAGTTTACTCCGAACGCTCTGTTCAAACGAATCAATCAGAAAGCAACTCGTAAGCATCGGCGCCGTCGCGAAACTCGGCGGCGCCCTCTCCGGTTTAAACGTCGATTGCTTGGAATCGGCGCTTTATATTCTCGATGTTTTGTCGTCCCTTCCAGAGGGGAGATTAGCGTTGAAAGATTGCGAAGATACGATTAGGAATTTGGTGAAATTGTTGATGAAAGTATCTGAGATTTGTACTCAATTTGCGTTGTCGATTTTGTGGGCGGTTTTCAAGCTAGCGCCGGAGGAATGTGCGGTGCTCGCGGTGGAGGCGGGATTGGCGGCGAAGCTGCTGCTTGTGATACAAAGTGGGTGCAATAAAGAGCTAAAACAGAGATCTTCTGAGCTATTGAAATTGTGTAGTCTAAATTACCCAGATTCTATCTTCATTTCTAAATGTAAGCTTACGAGAACTATACAATAA

Coding sequence (CDS)

ATGCCGATGTACCAGCCCTCTACTTGGAGACAAGGGGATCCCAAGCTCGTTGTGGGTTGCGGAGCGCAGATTCTAGATCTGGAAACTGTCGTCAAAGATGGGATTCTCGGCGGCGGCGGCGGCGGTGGGCTCATCTCCGGCGGTGGTGCTGAGAAATTGGATCTGAAGGAGATGATTGAGGAGCTGGAATCATCGATAGAGGTCCCATCGGTGTTCATTTGCCCAATTTCGCTCGAGCCGATGCAAGATCCAGTGACCCTTTGCTCCGGGCAGACCTACGAGCGGTCCAACATTCTGAAATGGTTTTCTTTAGGCCATTTAACTTGCCCCACAACAATGCAAGAGCTTTGGGACGATTCCGTCACACCGAATCGGACCCTTCACCAGTTGATTCACACCTGGTTTTTGCAGAAGTACTTGGCATTGAAGAAGAGATCAGAGGACGTTCAGGGCAGAGTTTTAGAGCTTTTGGATTCGTTGAAGAAGGTCAAGGGCCAAGCTAGGGTTCAGTCCCTTAAAGATCTCCGACATGTCGTCGCCACCCATTCCTCCGCCAAGAAAATGGTGGTCGAAAACGGCGGCGTCGAGCTAATCTCCTCTCTTTTGGGTTCTTTCACTTCTCACGCAGTTGGGTCCGAAGCCGTTGGGATCCTCGTCAATTTGGATCTCGATTCGGAGTTGAAGAAGAATCTGATTCAACCCACGAGAATGTCCCTAATTGCAGATATTTTAAACGAAGGGTCCATCGAAACCAAGATCAATTGCACGAAATTCATCAAGATGTTGATTGAGGGCGACGATTTAAGAACAGAGAACGTTTCAAGTTTGAGTCTACTCGTCGGATTGTTGAGAATGGTGAAGGATCAGAGACACCCAAATGGAGTAACCGCCGGACTCAGTTTACTCCGAACGCTCTGTTCAAACGAATCAATCAGAAAGCAACTCGTAAGCATCGGCGCCGTCGCGAAACTCGGCGGCGCCCTCTCCGGTTTAAACGTCGATTGCTTGGAATCGGCGCTTTATATTCTCGATGTTTTGTCGTCCCTTCCAGAGGGGAGATTAGCGTTGAAAGATTGCGAAGATACGATTAGGAATTTGGTGAAATTGTTGATGAAAGTATCTGAGATTTGTACTCAATTTGCGTTGTCGATTTTGTGGGCGGTTTTCAAGCTAGCGCCGGAGGAATGTGCGGTGCTCGCGGTGGAGGCGGGATTGGCGGCGAAGCTGCTGCTTGTGATACAAAGTGGGTGCAATAAAGAGCTAAAACAGAGATCTTCTGAGCTATTGAAATTGTGTAGTCTAAATTACCCAGATTCTATCTTCATTTCTAAATGTAAGCTTACGAGAACTATACAATAA

Protein sequence

MPMYQPSTWRQGDPKLVVGCGAQILDLETVVKDGILGGGGGGGLISGGGAEKLDLKEMIEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDSVTPNRTLHQLIHTWFLQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHVVATHSSAKKMVVENGGVELISSLLGSFTSHAVGSEAVGILVNLDLDSELKKNLIQPTRMSLIADILNEGSIETKINCTKFIKMLIEGDDLRTENVSSLSLLVGLLRMVKDQRHPNGVTAGLSLLRTLCSNESIRKQLVSIGAVAKLGGALSGLNVDCLESALYILDVLSSLPEGRLALKDCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQSGCNKELKQRSSELLKLCSLNYPDSIFISKCKLTRTIQ

Homology

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BLAST of Spg025222 vs. NCBI nr
Match: XP_038884660.1 (U-box domain-containing protein 31-like [Benincasa hispida])

HSP 1 Score: 827.8 bits (2137), Expect = 4.6e-236
Identity = 430/452 (95.13%), Postives = 440/452 (97.35%), Query Frame = 0


Query: 1   MPMYQPSTWRQGDPKLVVGCGAQILDLETVVKDGILGGGGGGGLISGGGAEKLDLKEMIE 60
           MPMYQP+TWR+GDPKLVVGCGAQILDLETVVKDGIL  GGGGG+I GGG EKLDLK+MIE
Sbjct: 1   MPMYQPATWREGDPKLVVGCGAQILDLETVVKDGIL--GGGGGIICGGG-EKLDLKDMIE 60

Query: 61  ELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDS 120
           ELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDS
Sbjct: 61  ELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDS 120

Query: 121 VTPNRTLHQLIHTWFLQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHVVA 180
           VTPNRTLHQLIHTWF QKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHVV 
Sbjct: 121 VTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHVVT 180

Query: 181 THSSAKKMVVENGGVELISSLLGSFTSHAVGSEAVGILVNLDLDSELKKNLIQPTRMSLI 240
           THSSAKKMVV+NGGV LISSLLGSFTSHAVGSEAVGILVNLDLDSELKKNLIQPTR+SLI
Sbjct: 181 THSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLDSELKKNLIQPTRISLI 240

Query: 241 ADILNEGSIETKINCTKFIKMLIEGDDLRTENVSSLSLLVGLLRMVKDQRHPNGVTAGLS 300
           ADILNEGSIETKINCTKFIKMLI G+DLRTENVSSLSLLVGLLRMVKDQRHPNGVTAGLS
Sbjct: 241 ADILNEGSIETKINCTKFIKMLIVGEDLRTENVSSLSLLVGLLRMVKDQRHPNGVTAGLS 300

Query: 301 LLRTLCSNESIRKQLVSIGAVAKLGGALSGLNVDCLESALYILDVLSSLPEGRLALKDCE 360
           LLRTLCSNE IRKQLVSIGAVAKLGGALS LN DCLESALYILDVLSSLPEGRLALK+CE
Sbjct: 301 LLRTLCSNEPIRKQLVSIGAVAKLGGALSNLNTDCLESALYILDVLSSLPEGRLALKNCE 360

Query: 361 DTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQSGCNKE 420
           DTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEEC+VLAVEAGLAAKLLLVIQSGCN E
Sbjct: 361 DTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECSVLAVEAGLAAKLLLVIQSGCNPE 420

Query: 421 LKQRSSELLKLCSLNYPDSIFISKCKLTRTIQ 453
           LKQRS+ELLKLCSLNYPDSIFISKCKLTRTIQ
Sbjct: 421 LKQRSAELLKLCSLNYPDSIFISKCKLTRTIQ 449

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BLAST of Spg025222 vs. NCBI nr
Match: XP_031736908.1 (U-box domain-containing protein 30 isoform X2 [Cucumis sativus])

HSP 1 Score: 814.7 bits (2103), Expect = 4.0e-232
Identity = 425/457 (93.00%), Postives = 437/457 (95.62%), Query Frame = 0


Query: 1   MPMYQP-STWRQGDPKLVVGCGAQILDLETVVKDGILGGGG----GGGLISGGGAEKLDL 60
           MPMYQP +TWR+GDPKLVVGCG QILDLETVVKDGILGGGG    GGG   GGG EKLDL
Sbjct: 1   MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGG--GGGGGEKLDL 60

Query: 61  KEMIEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE 120
           KEMIEELESS+EVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE
Sbjct: 61  KEMIEELESSVEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE 120

Query: 121 LWDDSVTPNRTLHQLIHTWFLQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL 180
           LWDDSVTPNRTLHQLIHTWF QKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL
Sbjct: 121 LWDDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL 180

Query: 181 RHVVATHSSAKKMVVENGGVELISSLLGSFTSHAVGSEAVGILVNLDLDSELKKNLIQPT 240
           RH+V THSSAKKMVV+NGGV LISSLLGSFTSHAVGSEAVGILVNLDL+SELKKNLIQPT
Sbjct: 181 RHIVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLNSELKKNLIQPT 240

Query: 241 RMSLIADILNEGSIETKINCTKFIKMLIEGDDLRTENVSSLSLLVGLLRMVKDQRHPNGV 300
           R+SLIADILNEGSIETKINCTKF KMLI G+DL+TE+VSSLSLLVGLLRMVKDQRHPNGV
Sbjct: 241 RISLIADILNEGSIETKINCTKFFKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNGV 300

Query: 301 TAGLSLLRTLCSNESIRKQLVSIGAVAKLGGALSGLNVDCLESALYILDVLSSLPEGRLA 360
           TAGLSLLRTLCSNE IRKQLVSIGAVAKLGG LS LN DCLESALYILDVLSSLPEGRLA
Sbjct: 301 TAGLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNTDCLESALYILDVLSSLPEGRLA 360

Query: 361 LKDCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS 420
           LK+CEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS
Sbjct: 361 LKNCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS 420

Query: 421 GCNKELKQRSSELLKLCSLNYPDSIFISKCKLTRTIQ 453
           GCN ELKQRS+ELLKLCSLNYPDSIFISKCKLTRTIQ
Sbjct: 421 GCNPELKQRSAELLKLCSLNYPDSIFISKCKLTRTIQ 455

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BLAST of Spg025222 vs. NCBI nr
Match: XP_011649559.1 (U-box domain-containing protein 30 isoform X1 [Cucumis sativus] >KAE8652062.1 hypothetical protein Csa_018635 [Cucumis sativus])

HSP 1 Score: 814.7 bits (2103), Expect = 4.0e-232
Identity = 425/457 (93.00%), Postives = 437/457 (95.62%), Query Frame = 0


Query: 1   MPMYQP-STWRQGDPKLVVGCGAQILDLETVVKDGILGGGG----GGGLISGGGAEKLDL 60
           MPMYQP +TWR+GDPKLVVGCG QILDLETVVKDGILGGGG    GGG   GGG EKLDL
Sbjct: 24  MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGG--GGGGGEKLDL 83

Query: 61  KEMIEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE 120
           KEMIEELESS+EVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE
Sbjct: 84  KEMIEELESSVEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE 143

Query: 121 LWDDSVTPNRTLHQLIHTWFLQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL 180
           LWDDSVTPNRTLHQLIHTWF QKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL
Sbjct: 144 LWDDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL 203

Query: 181 RHVVATHSSAKKMVVENGGVELISSLLGSFTSHAVGSEAVGILVNLDLDSELKKNLIQPT 240
           RH+V THSSAKKMVV+NGGV LISSLLGSFTSHAVGSEAVGILVNLDL+SELKKNLIQPT
Sbjct: 204 RHIVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLNSELKKNLIQPT 263

Query: 241 RMSLIADILNEGSIETKINCTKFIKMLIEGDDLRTENVSSLSLLVGLLRMVKDQRHPNGV 300
           R+SLIADILNEGSIETKINCTKF KMLI G+DL+TE+VSSLSLLVGLLRMVKDQRHPNGV
Sbjct: 264 RISLIADILNEGSIETKINCTKFFKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNGV 323

Query: 301 TAGLSLLRTLCSNESIRKQLVSIGAVAKLGGALSGLNVDCLESALYILDVLSSLPEGRLA 360
           TAGLSLLRTLCSNE IRKQLVSIGAVAKLGG LS LN DCLESALYILDVLSSLPEGRLA
Sbjct: 324 TAGLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNTDCLESALYILDVLSSLPEGRLA 383

Query: 361 LKDCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS 420
           LK+CEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS
Sbjct: 384 LKNCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS 443

Query: 421 GCNKELKQRSSELLKLCSLNYPDSIFISKCKLTRTIQ 453
           GCN ELKQRS+ELLKLCSLNYPDSIFISKCKLTRTIQ
Sbjct: 444 GCNPELKQRSAELLKLCSLNYPDSIFISKCKLTRTIQ 478

[Close]

BLAST of Spg025222 vs. NCBI nr
Match: XP_008444531.1 (PREDICTED: U-box domain-containing protein 30-like [Cucumis melo] >KAA0060963.1 U-box domain-containing protein 30-like [Cucumis melo var. makuwa])

HSP 1 Score: 812.4 bits (2097), Expect = 2.0e-231
Identity = 426/457 (93.22%), Postives = 437/457 (95.62%), Query Frame = 0


Query: 1   MPMYQP-STWRQGDPKLVVGCGAQILDLETVVKDGILGGGG----GGGLISGGGAEKLDL 60
           MPMYQP +TWR+GDPKLVVGCG QILDLETVVKDGILGGGG    GGG   GGG EKLDL
Sbjct: 1   MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGG--GGGGGEKLDL 60

Query: 61  KEMIEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE 120
           KEMIEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE
Sbjct: 61  KEMIEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE 120

Query: 121 LWDDSVTPNRTLHQLIHTWFLQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL 180
           LWDDSVTPNRTLHQLIHTWF QKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL
Sbjct: 121 LWDDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL 180

Query: 181 RHVVATHSSAKKMVVENGGVELISSLLGSFTSHAVGSEAVGILVNLDLDSELKKNLIQPT 240
           R +V THSSAKKMVV+NGGV LISSLLGSFTSHAVGSEAVGILVNLDL+SELKKNLIQPT
Sbjct: 181 RLIVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLNSELKKNLIQPT 240

Query: 241 RMSLIADILNEGSIETKINCTKFIKMLIEGDDLRTENVSSLSLLVGLLRMVKDQRHPNGV 300
           R+SLIADILNEGSIETKINCTKFIKMLI G+DL+TE+VSSLSLLVGLLRMVKDQRHPNGV
Sbjct: 241 RISLIADILNEGSIETKINCTKFIKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNGV 300

Query: 301 TAGLSLLRTLCSNESIRKQLVSIGAVAKLGGALSGLNVDCLESALYILDVLSSLPEGRLA 360
           TAGLSLLRTLCSNE IRKQLVSIGAVAKLGG LS LN DCLESALYILDVLSSLPEGRLA
Sbjct: 301 TAGLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNADCLESALYILDVLSSLPEGRLA 360

Query: 361 LKDCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS 420
           LK+CEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS
Sbjct: 361 LKNCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS 420

Query: 421 GCNKELKQRSSELLKLCSLNYPDSIFISKCKLTRTIQ 453
           GCN ELKQRS+ELLKLCSLNYPDSIFISKCKLTRTIQ
Sbjct: 421 GCNPELKQRSAELLKLCSLNYPDSIFISKCKLTRTIQ 455

[Close]

BLAST of Spg025222 vs. NCBI nr
Match: XP_022962495.1 (U-box domain-containing protein 31-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 791.6 bits (2043), Expect = 3.6e-225
Identity = 411/452 (90.93%), Postives = 427/452 (94.47%), Query Frame = 0


Query: 1   MPMYQPSTWRQGDPKLVVGCGAQILDLETVVKDGILGGGGGGGLISGGGAEKLDLKEMIE 60
           MP+Y PS   Q DPKL+VGCGA ILDL+TVVKDGILGGGGGG + + GGA+KLDLKEMIE
Sbjct: 1   MPIYHPSICTQRDPKLLVGCGAHILDLQTVVKDGILGGGGGGVIAADGGAQKLDLKEMIE 60

Query: 61  ELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDS 120
           ELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDS
Sbjct: 61  ELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDS 120

Query: 121 VTPNRTLHQLIHTWFLQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHVVA 180
           VTPNRTLHQLIHTWF QKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHVV 
Sbjct: 121 VTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHVVT 180

Query: 181 THSSAKKMVVENGGVELISSLLGSFTSHAVGSEAVGILVNLDLDSELKKNLIQPTRMSLI 240
            HSSAKKMVV+NGGV LISSLLGSFTSHAVGSE VGILVNLDL+SELKKNLIQPTR+SLI
Sbjct: 181 AHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEGVGILVNLDLNSELKKNLIQPTRISLI 240

Query: 241 ADILNEGSIETKINCTKFIKMLIEGDDLRTENVSSLSLLVGLLRMVKDQRHPNGVTAGLS 300
           ADILNEGSIETKINCTKFIKML EGD    ENVSSLSLLVGLLRMVKDQRHPNGVTA L+
Sbjct: 241 ADILNEGSIETKINCTKFIKMLTEGDASTIENVSSLSLLVGLLRMVKDQRHPNGVTAALA 300

Query: 301 LLRTLCSNESIRKQLVSIGAVAKLGGALSGLNVDCLESALYILDVLSSLPEGRLALKDCE 360
           LLRTLCSNESIRKQLV IGAVAKLGGALSGLN + LESALYILDVLSSLPEGRLALK+CE
Sbjct: 301 LLRTLCSNESIRKQLVGIGAVAKLGGALSGLNAESLESALYILDVLSSLPEGRLALKNCE 360

Query: 361 DTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQSGCNKE 420
           DT+RNLVKLLMKVSEICT+FALSILWAVFKLAPEEC+VLAVEAGLAAKLLLVIQSG N E
Sbjct: 361 DTVRNLVKLLMKVSEICTRFALSILWAVFKLAPEECSVLAVEAGLAAKLLLVIQSGFNPE 420

Query: 421 LKQRSSELLKLCSLNYPDSIFISKCKLTRTIQ 453
           LKQRS+ELLKLCSLNYPDSIFISKCKLTRTIQ
Sbjct: 421 LKQRSAELLKLCSLNYPDSIFISKCKLTRTIQ 452

[Close]

BLAST of Spg025222 vs. ExPASy Swiss-Prot
Match: Q058P4 (U-box domain-containing protein 30 OS=Arabidopsis thaliana OX=3702 GN=PUB30 PE=1 SV=1)

HSP 1 Score: 550.4 bits (1417), Expect = 1.8e-155
Identity = 290/452 (64.16%), Postives = 356/452 (78.76%), Query Frame = 0


Query: 1   MPMYQPSTWRQGDPKLVVGCGAQILDLETVVKDGILGGGGGGGLISGGGAEKLDLKEMIE 60
           MPM+QP   R G      G   Q+LDL+T VKDG+LGG  GGG+  G   EKLDLK+MI+
Sbjct: 1   MPMFQPLK-RDGLIGFEGGGDGQVLDLDTAVKDGVLGGVNGGGV--GVVDEKLDLKKMIK 60

Query: 61  ELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDS 120
           EL+   ++PSVFICPISLEPMQDPVTLC+GQTYER NI KWF+LGHLTCPTTMQELWDD+
Sbjct: 61  ELDLQ-DIPSVFICPISLEPMQDPVTLCTGQTYERLNIHKWFNLGHLTCPTTMQELWDDT 120

Query: 121 VTPNRTLHQLIHTWFLQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHVVA 180
           VTPN+TLH LI+TWF QKY+ +KKRSEDVQGR +E+L +LKK KGQARV +L +L+ +V 
Sbjct: 121 VTPNKTLHHLIYTWFSQKYVLMKKRSEDVQGRAIEILGTLKKAKGQARVHALSELKQIVI 180

Query: 181 THSSAKKMVVENGGVELISSLLGSFTSHAVGSEAVGILVNLDLDSELKKNLIQPTRMSLI 240
            H  A+K VVE GGV +ISSLLG FTSHAVGSE V ILV+LDLDS+ K  L+QP ++SLI
Sbjct: 181 AHLMARKTVVEEGGVSVISSLLGPFTSHAVGSEVVAILVSLDLDSDSKSGLMQPAKVSLI 240

Query: 241 ADILNEGSIETKINCTKFIKMLIEGDDLRTENVSSLSLLVGLLRMVKDQRHPNGVTAGLS 300
            D+LN+GS ETKINC + I+ L+E    R E VSS SLLVGL+R+VKD+RH NGV+  L 
Sbjct: 241 VDMLNDGSNETKINCARLIRGLVEEKGFRAELVSSHSLLVGLMRLVKDKRHRNGVSPALR 300

Query: 301 LLRTLCSNESIRKQLVSIGAVAKLGGALSGLNVDCLESALYILDVLSSLPEGRLALKDCE 360
           LL+ +  ++ +R  +VSIGAV +L   L  L+ +CLE AL++LD L +  EGR+A+KD  
Sbjct: 301 LLKPISVHKQVRSLMVSIGAVPQLVDILPSLDPECLELALFVLDALCTDVEGRVAVKDSA 360

Query: 361 DTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQSGCNKE 420
           +TI   V++LM+VSE CT +ALSILW+V KLAPEEC+ LAVE GLAAKLLLVIQSGC+  
Sbjct: 361 NTIPYTVRVLMRVSENCTNYALSILWSVCKLAPEECSPLAVEVGLAAKLLLVIQSGCDAA 420

Query: 421 LKQRSSELLKLCSLNYPDSIFISKCKLTRTIQ 453
           LKQRS+ELLKLCSL+Y D++FISKCKLTRTIQ
Sbjct: 421 LKQRSAELLKLCSLHYSDTMFISKCKLTRTIQ 448

[Close]

BLAST of Spg025222 vs. ExPASy Swiss-Prot
Match: Q9FHN9 (U-box domain-containing protein 31 OS=Arabidopsis thaliana OX=3702 GN=PUB31 PE=2 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 2.0e-154
Identity = 292/453 (64.46%), Postives = 356/453 (78.59%), Query Frame = 0


Query: 1   MPMYQPSTWRQGDPKLVVGCGAQILDLETVVKDGILGGGGGGGL-ISGGGAEKLDLKEMI 60
           MPM+QPS  + G      G    ILDL + VKDG+LGGG G  L +     +KLDLKEMI
Sbjct: 1   MPMFQPS--KNG------GFDGHILDLHSAVKDGVLGGGDGKFLVVVTDEKKKLDLKEMI 60

Query: 61  EELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDD 120
            ELE   E+PSVFICPISLEPMQDPVTLC+GQTYERSNILKWF++GH TCPTTMQELWDD
Sbjct: 61  SELELP-EIPSVFICPISLEPMQDPVTLCTGQTYERSNILKWFNIGHCTCPTTMQELWDD 120

Query: 121 SVTPNRTLHQLIHTWFLQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHVV 180
            VTPN+TLHQLI+TWF QKY+ +KKRSEDVQGR +E+L +L+K KG+A+V +L +L+ VV
Sbjct: 121 LVTPNKTLHQLIYTWFSQKYVLMKKRSEDVQGRAIEILGTLRKAKGKAKVHALSELKQVV 180

Query: 181 ATHSSAKKMVVENGGVELISSLLGSFTSHAVGSEAVGILVNLDLDSELKKNLIQPTRMSL 240
             H+ AKK VV+ GGV +ISSLL  FTSHAVGSEA+ ILVNL+LDS+ K  L+QP R+SL
Sbjct: 181 MAHAIAKKTVVDEGGVFVISSLLSPFTSHAVGSEAIAILVNLELDSDSKAGLMQPARVSL 240

Query: 241 IADILNEGSIETKINCTKFIKMLIEGDDLRTENVSSLSLLVGLLRMVKDQRHPNGVTAGL 300
           + D+LN+GSIETKINC + I  L+E    R E VSS SLLVGL+R+VKD+R  NGV+  L
Sbjct: 241 MVDMLNDGSIETKINCARLIGRLVEEKGFRAELVSSHSLLVGLMRLVKDRRRRNGVSPAL 300

Query: 301 SLLRTLCSNESIRKQLVSIGAVAKLGGALSGLNVDCLESALYILDVLSSLPEGRLALKDC 360
           +LL+++  ++ +R  LV IGAV +L   L  L+V+CLESAL++LD L    EGR+ALKD 
Sbjct: 301 TLLKSVSVHKQVRNLLVRIGAVPQLVDVLPCLDVECLESALFVLDSLCLESEGRIALKDS 360

Query: 361 EDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQSGCNK 420
            +TI + V+LLMKVSE CT +A+SILW+V KLA EEC+ LAVE GLAAKLLLVIQSGC+ 
Sbjct: 361 VNTIPHTVRLLMKVSEKCTNYAISILWSVCKLASEECSSLAVEVGLAAKLLLVIQSGCDP 420

Query: 421 ELKQRSSELLKLCSLNYPDSIFISKCKLTRTIQ 453
            LKQRS+ELLKLCSL+Y DS+FISKCKLTRTIQ
Sbjct: 421 ALKQRSAELLKLCSLHYSDSMFISKCKLTRTIQ 444

[Close]

BLAST of Spg025222 vs. ExPASy Swiss-Prot
Match: Q10PI9 (U-box domain-containing protein 75 OS=Oryza sativa subsp. japonica OX=39947 GN=TUD1 PE=1 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 6.2e-135
Identity = 251/451 (55.65%), Postives = 337/451 (74.72%), Query Frame = 0


Query: 20  CGAQILDLETVVKDGILGGGG--GGGLISGGGAEKLDLKEMIEELESSIE------VPSV 79
           CG Q+LD++T +KDGILGGG   G      GG + ++L++M++EL+++ +      VP+V
Sbjct: 9   CGGQVLDIDTALKDGILGGGPELGDAAAGDGGKQPVELRKMMDELDAAGDGGGDEAVPAV 68

Query: 80  FICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDSVTPNRTLHQLI 139
           FICPISLEPM DPVTLC+GQTYE +NI +W +LGH TCPTTMQELWD +  PN TL QLI
Sbjct: 69  FICPISLEPMVDPVTLCTGQTYESANISRWLALGHRTCPTTMQELWDVTPIPNTTLRQLI 128

Query: 140 HTWFLQKYLALKKRSEDVQGRVLELLDSLK--------KVKGQARVQSLKDLRHVVATHS 199
             WF ++Y   KKRS D  GR  EL+ +L+         +KGQARV +L++LR + A H 
Sbjct: 129 AAWFSRRYTRFKKRSADFHGRAAELVHALRGTAVPKRQPLKGQARVAALRELRSLAAAHQ 188

Query: 200 SAKKMVVENGGVELISSLLGSFTSHAVGSEAVGILVN-LDLDSELKKNLIQPTRMSLIAD 259
           S  K + E GGV L++SLLG FTSHAVGSEAV ILV+ + LD++ K  L+QP ++SL+ D
Sbjct: 189 SVTKAIAEAGGVGLLTSLLGPFTSHAVGSEAVAILVSGVPLDADAKAALMQPAKVSLLVD 248

Query: 260 ILNEGSIETKINCTKFIKMLIEGDDLRTENVSSLSLLVGLLRMVKDQRHPNGVTAGLSLL 319
           +LNEG+++TKINC + I++L+E    R + V+SLSLLVG++R+V+D+RHP+GV AGL LL
Sbjct: 249 MLNEGAVDTKINCVRLIRILMEEKGFRPDTVASLSLLVGVMRLVRDKRHPDGVAAGLELL 308

Query: 320 RTLCS-NESIRKQLVSIGAVAKLGGALSGLNVDCLESALYILDVLSSLPEGRLALKDCED 379
            ++C+ ++  R  +VSIGAV +L   L  L  +C+E AL ILD L+++PEGR+ALKDC  
Sbjct: 309 NSICAVHKPARSLIVSIGAVPQLVELLPELPTECVEPALDILDALAAVPEGRIALKDCPR 368

Query: 380 TIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQSGCNKEL 439
           TI N V+LLM+VSE CT+ ALS+LW V ++APEECA  A++AGL AKLLLVIQSGC  EL
Sbjct: 369 TITNAVRLLMRVSEACTRRALSMLWVVCRMAPEECAPAALDAGLGAKLLLVIQSGCGPEL 428

Query: 440 KQRSSELLKLCSLNYPDSIFISKCKLTRTIQ 453
           KQ++SELLKLC++N   ++FISKCKLT+TIQ
Sbjct: 429 KQQASELLKLCTMNCTSTVFISKCKLTKTIQ 459

[Close]

BLAST of Spg025222 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 1.6e-37
Identity = 126/400 (31.50%), Postives = 205/400 (51.25%), Query Frame = 0


Query: 59  IEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLG-HLTCPTTMQELW 118
           IE L+  I++P  F CPISLE MQDPVT+C+GQTY+R++I  W S+G + TCP T   L 
Sbjct: 5   IEPLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS 64

Query: 119 DDSVTPNRTLHQLIHTWFLQ------KYLALKKRSEDVQGRVLELLDSLKKVKG-----Q 178
           D ++ PN TL +LI  W +       + +   K+  D    V  LL     + G     +
Sbjct: 65  DFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTS-VRALLSQASAITGTHVSVR 124

Query: 179 ARVQSLKDLRHVVATHSSAKKMVVENGGVE-LISSLLGSFTSHAVGSEAVGILVNLDL-- 238
           +R  +L+ LR         + ++  +   E LI  L    TS  + SE++ +LV L +  
Sbjct: 125 SRAAALRRLRGFARDSDKNRVLIAAHNATEILIKILFSETTSSELVSESLALLVMLPITE 184

Query: 239 DSELKKNLIQPTRMSLIADILNEGSIETKINCTKFIKMLIEGD---DLRTENVSSLSLLV 298
            ++       P R+  +  +L + SIET++N    I+++  G    DL+    +S S+  
Sbjct: 185 PNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESVFE 244

Query: 299 GLLRMVKDQ-RHPNGVTAGLSLLRTLCSNESIRKQLVSIGAVAKLGGALSGLNVDC-LES 358
           G+L ++++       +  G+  L  LCS +S R   ++ GA   L   L+     C  E 
Sbjct: 245 GVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCDTER 304

Query: 359 ALYILDVLSSLPEGRLALKDCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAV 418
           AL  +++L   PEG  A  +   T+  LVK +++VS+  T++A   L A+   A E    
Sbjct: 305 ALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALC-TAEERWRE 364

Query: 419 LAVEAGLAAKLLLVIQSGCNKELKQRSSELLKLCSLNYPD 439
            A  AG+  +LLL++QS C +  K+++ +LLKL   ++PD
Sbjct: 365 EAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSWPD 402

[Close]

BLAST of Spg025222 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 3.9e-36
Identity = 119/404 (29.46%), Postives = 208/404 (51.49%), Query Frame = 0


Query: 59  IEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWD 118
           +E L+  I++P  F CPISL+ M DPVT+ +GQTY+R++I  W ++G+ TCP T   L D
Sbjct: 5   LEPLDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTTCPVTRVALSD 64

Query: 119 DSVTPNRTLHQLIHTWF-------LQKYLALKKRSEDVQGRVLELLDSLKKVKG-----Q 178
            ++ PN TL +LI  W        +++    K+ ++ +   V  LL     + G     +
Sbjct: 65  FTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPIS--VRSLLSQASAITGTHVSVR 124

Query: 179 ARVQSLKDLRHVVATHSSAKKMVVENGGVELISSLL------GSFTSHAVGSEAVGILVN 238
           +R  +++ LR +       + ++  +   E++  +L       S +S  V SE++ +LV 
Sbjct: 125 SRAAAIRRLRGLARDSEKNRVLIAGHNAREILVRILFADIETTSLSSELV-SESLALLVL 184

Query: 239 LDL-DSELKKNLIQPTRMSLIADILNEGSIETKINCTKFIKMLIEGD---DLRTENVSSL 298
           L + ++E +     P+R+  +  +L + SIE ++N    I+M++ G    DL+     S 
Sbjct: 185 LHMTETECEAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSD 244

Query: 299 SLLVGLLRMVKDQ-RHPNGVTAGLSLLRTLCSNESIRKQLVSIGAVAKLGGALSGLNVDC 358
           S+  G+L ++K+       +  G+  +  LC  +  R   +S GA   L   L+     C
Sbjct: 245 SIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADFDRC 304

Query: 359 -LESALYILDVLSSLPEGRLALKDCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPE 418
             E  L  +++L  LPEG  A  +   T+  +VK +++VS+  T++A   L A+   A E
Sbjct: 305 DTERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALC-TAEE 364

Query: 419 ECAVLAVEAGLAAKLLLVIQSGCNKELKQRSSELLKLCSLNYPD 439
            C   A  AGL  +LLL++QS C +  K+++  LLKL   ++PD
Sbjct: 365 RCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDSWPD 404

[Close]

BLAST of Spg025222 vs. ExPASy TrEMBL
Match: A0A0A0LKY4 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_2G354100 PE=4 SV=1)

HSP 1 Score: 814.7 bits (2103), Expect = 1.9e-232
Identity = 425/457 (93.00%), Postives = 437/457 (95.62%), Query Frame = 0


Query: 1   MPMYQP-STWRQGDPKLVVGCGAQILDLETVVKDGILGGGG----GGGLISGGGAEKLDL 60
           MPMYQP +TWR+GDPKLVVGCG QILDLETVVKDGILGGGG    GGG   GGG EKLDL
Sbjct: 1   MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGG--GGGGGEKLDL 60

Query: 61  KEMIEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE 120
           KEMIEELESS+EVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE
Sbjct: 61  KEMIEELESSVEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE 120

Query: 121 LWDDSVTPNRTLHQLIHTWFLQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL 180
           LWDDSVTPNRTLHQLIHTWF QKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL
Sbjct: 121 LWDDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL 180

Query: 181 RHVVATHSSAKKMVVENGGVELISSLLGSFTSHAVGSEAVGILVNLDLDSELKKNLIQPT 240
           RH+V THSSAKKMVV+NGGV LISSLLGSFTSHAVGSEAVGILVNLDL+SELKKNLIQPT
Sbjct: 181 RHIVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLNSELKKNLIQPT 240

Query: 241 RMSLIADILNEGSIETKINCTKFIKMLIEGDDLRTENVSSLSLLVGLLRMVKDQRHPNGV 300
           R+SLIADILNEGSIETKINCTKF KMLI G+DL+TE+VSSLSLLVGLLRMVKDQRHPNGV
Sbjct: 241 RISLIADILNEGSIETKINCTKFFKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNGV 300

Query: 301 TAGLSLLRTLCSNESIRKQLVSIGAVAKLGGALSGLNVDCLESALYILDVLSSLPEGRLA 360
           TAGLSLLRTLCSNE IRKQLVSIGAVAKLGG LS LN DCLESALYILDVLSSLPEGRLA
Sbjct: 301 TAGLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNTDCLESALYILDVLSSLPEGRLA 360

Query: 361 LKDCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS 420
           LK+CEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS
Sbjct: 361 LKNCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS 420

Query: 421 GCNKELKQRSSELLKLCSLNYPDSIFISKCKLTRTIQ 453
           GCN ELKQRS+ELLKLCSLNYPDSIFISKCKLTRTIQ
Sbjct: 421 GCNPELKQRSAELLKLCSLNYPDSIFISKCKLTRTIQ 455

[Close]

BLAST of Spg025222 vs. ExPASy TrEMBL
Match: A0A5A7UYA8 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00980 PE=4 SV=1)

HSP 1 Score: 812.4 bits (2097), Expect = 9.6e-232
Identity = 426/457 (93.22%), Postives = 437/457 (95.62%), Query Frame = 0


Query: 1   MPMYQP-STWRQGDPKLVVGCGAQILDLETVVKDGILGGGG----GGGLISGGGAEKLDL 60
           MPMYQP +TWR+GDPKLVVGCG QILDLETVVKDGILGGGG    GGG   GGG EKLDL
Sbjct: 1   MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGG--GGGGGEKLDL 60

Query: 61  KEMIEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE 120
           KEMIEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE
Sbjct: 61  KEMIEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE 120

Query: 121 LWDDSVTPNRTLHQLIHTWFLQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL 180
           LWDDSVTPNRTLHQLIHTWF QKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL
Sbjct: 121 LWDDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL 180

Query: 181 RHVVATHSSAKKMVVENGGVELISSLLGSFTSHAVGSEAVGILVNLDLDSELKKNLIQPT 240
           R +V THSSAKKMVV+NGGV LISSLLGSFTSHAVGSEAVGILVNLDL+SELKKNLIQPT
Sbjct: 181 RLIVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLNSELKKNLIQPT 240

Query: 241 RMSLIADILNEGSIETKINCTKFIKMLIEGDDLRTENVSSLSLLVGLLRMVKDQRHPNGV 300
           R+SLIADILNEGSIETKINCTKFIKMLI G+DL+TE+VSSLSLLVGLLRMVKDQRHPNGV
Sbjct: 241 RISLIADILNEGSIETKINCTKFIKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNGV 300

Query: 301 TAGLSLLRTLCSNESIRKQLVSIGAVAKLGGALSGLNVDCLESALYILDVLSSLPEGRLA 360
           TAGLSLLRTLCSNE IRKQLVSIGAVAKLGG LS LN DCLESALYILDVLSSLPEGRLA
Sbjct: 301 TAGLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNADCLESALYILDVLSSLPEGRLA 360

Query: 361 LKDCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS 420
           LK+CEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS
Sbjct: 361 LKNCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS 420

Query: 421 GCNKELKQRSSELLKLCSLNYPDSIFISKCKLTRTIQ 453
           GCN ELKQRS+ELLKLCSLNYPDSIFISKCKLTRTIQ
Sbjct: 421 GCNPELKQRSAELLKLCSLNYPDSIFISKCKLTRTIQ 455

[Close]

BLAST of Spg025222 vs. ExPASy TrEMBL
Match: A0A1S3BA20 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103487822 PE=4 SV=1)

HSP 1 Score: 812.4 bits (2097), Expect = 9.6e-232
Identity = 426/457 (93.22%), Postives = 437/457 (95.62%), Query Frame = 0


Query: 1   MPMYQP-STWRQGDPKLVVGCGAQILDLETVVKDGILGGGG----GGGLISGGGAEKLDL 60
           MPMYQP +TWR+GDPKLVVGCG QILDLETVVKDGILGGGG    GGG   GGG EKLDL
Sbjct: 1   MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGG--GGGGGEKLDL 60

Query: 61  KEMIEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE 120
           KEMIEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE
Sbjct: 61  KEMIEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE 120

Query: 121 LWDDSVTPNRTLHQLIHTWFLQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL 180
           LWDDSVTPNRTLHQLIHTWF QKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL
Sbjct: 121 LWDDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL 180

Query: 181 RHVVATHSSAKKMVVENGGVELISSLLGSFTSHAVGSEAVGILVNLDLDSELKKNLIQPT 240
           R +V THSSAKKMVV+NGGV LISSLLGSFTSHAVGSEAVGILVNLDL+SELKKNLIQPT
Sbjct: 181 RLIVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLNSELKKNLIQPT 240

Query: 241 RMSLIADILNEGSIETKINCTKFIKMLIEGDDLRTENVSSLSLLVGLLRMVKDQRHPNGV 300
           R+SLIADILNEGSIETKINCTKFIKMLI G+DL+TE+VSSLSLLVGLLRMVKDQRHPNGV
Sbjct: 241 RISLIADILNEGSIETKINCTKFIKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNGV 300

Query: 301 TAGLSLLRTLCSNESIRKQLVSIGAVAKLGGALSGLNVDCLESALYILDVLSSLPEGRLA 360
           TAGLSLLRTLCSNE IRKQLVSIGAVAKLGG LS LN DCLESALYILDVLSSLPEGRLA
Sbjct: 301 TAGLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNADCLESALYILDVLSSLPEGRLA 360

Query: 361 LKDCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS 420
           LK+CEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS
Sbjct: 361 LKNCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS 420

Query: 421 GCNKELKQRSSELLKLCSLNYPDSIFISKCKLTRTIQ 453
           GCN ELKQRS+ELLKLCSLNYPDSIFISKCKLTRTIQ
Sbjct: 421 GCNPELKQRSAELLKLCSLNYPDSIFISKCKLTRTIQ 455

[Close]

BLAST of Spg025222 vs. ExPASy TrEMBL
Match: A0A6J1HFA1 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111462914 PE=4 SV=1)

HSP 1 Score: 791.6 bits (2043), Expect = 1.8e-225
Identity = 411/452 (90.93%), Postives = 427/452 (94.47%), Query Frame = 0


Query: 1   MPMYQPSTWRQGDPKLVVGCGAQILDLETVVKDGILGGGGGGGLISGGGAEKLDLKEMIE 60
           MP+Y PS   Q DPKL+VGCGA ILDL+TVVKDGILGGGGGG + + GGA+KLDLKEMIE
Sbjct: 1   MPIYHPSICTQRDPKLLVGCGAHILDLQTVVKDGILGGGGGGVIAADGGAQKLDLKEMIE 60

Query: 61  ELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDS 120
           ELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDS
Sbjct: 61  ELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDS 120

Query: 121 VTPNRTLHQLIHTWFLQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHVVA 180
           VTPNRTLHQLIHTWF QKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHVV 
Sbjct: 121 VTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHVVT 180

Query: 181 THSSAKKMVVENGGVELISSLLGSFTSHAVGSEAVGILVNLDLDSELKKNLIQPTRMSLI 240
            HSSAKKMVV+NGGV LISSLLGSFTSHAVGSE VGILVNLDL+SELKKNLIQPTR+SLI
Sbjct: 181 AHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEGVGILVNLDLNSELKKNLIQPTRISLI 240

Query: 241 ADILNEGSIETKINCTKFIKMLIEGDDLRTENVSSLSLLVGLLRMVKDQRHPNGVTAGLS 300
           ADILNEGSIETKINCTKFIKML EGD    ENVSSLSLLVGLLRMVKDQRHPNGVTA L+
Sbjct: 241 ADILNEGSIETKINCTKFIKMLTEGDASTIENVSSLSLLVGLLRMVKDQRHPNGVTAALA 300

Query: 301 LLRTLCSNESIRKQLVSIGAVAKLGGALSGLNVDCLESALYILDVLSSLPEGRLALKDCE 360
           LLRTLCSNESIRKQLV IGAVAKLGGALSGLN + LESALYILDVLSSLPEGRLALK+CE
Sbjct: 301 LLRTLCSNESIRKQLVGIGAVAKLGGALSGLNAESLESALYILDVLSSLPEGRLALKNCE 360

Query: 361 DTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQSGCNKE 420
           DT+RNLVKLLMKVSEICT+FALSILWAVFKLAPEEC+VLAVEAGLAAKLLLVIQSG N E
Sbjct: 361 DTVRNLVKLLMKVSEICTRFALSILWAVFKLAPEECSVLAVEAGLAAKLLLVIQSGFNPE 420

Query: 421 LKQRSSELLKLCSLNYPDSIFISKCKLTRTIQ 453
           LKQRS+ELLKLCSLNYPDSIFISKCKLTRTIQ
Sbjct: 421 LKQRSAELLKLCSLNYPDSIFISKCKLTRTIQ 452

[Close]

BLAST of Spg025222 vs. ExPASy TrEMBL
Match: A0A6J1K7W0 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492478 PE=4 SV=1)

HSP 1 Score: 787.3 bits (2032), Expect = 3.3e-224
Identity = 409/452 (90.49%), Postives = 425/452 (94.03%), Query Frame = 0


Query: 1   MPMYQPSTWRQGDPKLVVGCGAQILDLETVVKDGILGGGGGGGLISGGGAEKLDLKEMIE 60
           MP+Y PS   Q DPKL+V CGA ILDL+TVVKDGILGGGGGG + + GGA+KLDLKEMIE
Sbjct: 1   MPIYHPSICTQRDPKLLVDCGAHILDLQTVVKDGILGGGGGGVIATDGGAQKLDLKEMIE 60

Query: 61  ELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDS 120
           ELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDS
Sbjct: 61  ELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDS 120

Query: 121 VTPNRTLHQLIHTWFLQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHVVA 180
           VTPNRTLHQLIHTWF QKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHVV 
Sbjct: 121 VTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHVVT 180

Query: 181 THSSAKKMVVENGGVELISSLLGSFTSHAVGSEAVGILVNLDLDSELKKNLIQPTRMSLI 240
            HSS KKMVV+NGGV LISSLLGSFTSHAVGSE VGILVNLDL+SELKKNLIQPTR+SLI
Sbjct: 181 AHSSVKKMVVDNGGVALISSLLGSFTSHAVGSEGVGILVNLDLNSELKKNLIQPTRISLI 240

Query: 241 ADILNEGSIETKINCTKFIKMLIEGDDLRTENVSSLSLLVGLLRMVKDQRHPNGVTAGLS 300
           ADILNEGSIETKINCTKFIKML EGD    ENVSSLSLLVGLLRMVKDQRHPNGVTA L+
Sbjct: 241 ADILNEGSIETKINCTKFIKMLTEGDASTIENVSSLSLLVGLLRMVKDQRHPNGVTAALT 300

Query: 301 LLRTLCSNESIRKQLVSIGAVAKLGGALSGLNVDCLESALYILDVLSSLPEGRLALKDCE 360
           LLRTLCSNESIRKQLV IGAVAKLGGALSGLN + LESALYILDVLSSLPEGRLALK+CE
Sbjct: 301 LLRTLCSNESIRKQLVGIGAVAKLGGALSGLNAESLESALYILDVLSSLPEGRLALKNCE 360

Query: 361 DTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQSGCNKE 420
           DT+RNLVKLLMKVSEICT+FALSILWAVFKLAPEEC+VLAVEAGLAAKLLLVIQSG N E
Sbjct: 361 DTVRNLVKLLMKVSEICTRFALSILWAVFKLAPEECSVLAVEAGLAAKLLLVIQSGFNPE 420

Query: 421 LKQRSSELLKLCSLNYPDSIFISKCKLTRTIQ 453
           LKQRS+ELLKLCSLNYPDSIFISKCKLTRTIQ
Sbjct: 421 LKQRSAELLKLCSLNYPDSIFISKCKLTRTIQ 452

[Close]

BLAST of Spg025222 vs. TAIR 10
Match: AT3G49810.1 (ARM repeat superfamily protein )

HSP 1 Score: 550.4 bits (1417), Expect = 1.3e-156
Identity = 290/452 (64.16%), Postives = 356/452 (78.76%), Query Frame = 0


Query: 1   MPMYQPSTWRQGDPKLVVGCGAQILDLETVVKDGILGGGGGGGLISGGGAEKLDLKEMIE 60
           MPM+QP   R G      G   Q+LDL+T VKDG+LGG  GGG+  G   EKLDLK+MI+
Sbjct: 1   MPMFQPLK-RDGLIGFEGGGDGQVLDLDTAVKDGVLGGVNGGGV--GVVDEKLDLKKMIK 60

Query: 61  ELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDS 120
           EL+   ++PSVFICPISLEPMQDPVTLC+GQTYER NI KWF+LGHLTCPTTMQELWDD+
Sbjct: 61  ELDLQ-DIPSVFICPISLEPMQDPVTLCTGQTYERLNIHKWFNLGHLTCPTTMQELWDDT 120

Query: 121 VTPNRTLHQLIHTWFLQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHVVA 180
           VTPN+TLH LI+TWF QKY+ +KKRSEDVQGR +E+L +LKK KGQARV +L +L+ +V 
Sbjct: 121 VTPNKTLHHLIYTWFSQKYVLMKKRSEDVQGRAIEILGTLKKAKGQARVHALSELKQIVI 180

Query: 181 THSSAKKMVVENGGVELISSLLGSFTSHAVGSEAVGILVNLDLDSELKKNLIQPTRMSLI 240
            H  A+K VVE GGV +ISSLLG FTSHAVGSE V ILV+LDLDS+ K  L+QP ++SLI
Sbjct: 181 AHLMARKTVVEEGGVSVISSLLGPFTSHAVGSEVVAILVSLDLDSDSKSGLMQPAKVSLI 240

Query: 241 ADILNEGSIETKINCTKFIKMLIEGDDLRTENVSSLSLLVGLLRMVKDQRHPNGVTAGLS 300
            D+LN+GS ETKINC + I+ L+E    R E VSS SLLVGL+R+VKD+RH NGV+  L 
Sbjct: 241 VDMLNDGSNETKINCARLIRGLVEEKGFRAELVSSHSLLVGLMRLVKDKRHRNGVSPALR 300

Query: 301 LLRTLCSNESIRKQLVSIGAVAKLGGALSGLNVDCLESALYILDVLSSLPEGRLALKDCE 360
           LL+ +  ++ +R  +VSIGAV +L   L  L+ +CLE AL++LD L +  EGR+A+KD  
Sbjct: 301 LLKPISVHKQVRSLMVSIGAVPQLVDILPSLDPECLELALFVLDALCTDVEGRVAVKDSA 360

Query: 361 DTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQSGCNKE 420
           +TI   V++LM+VSE CT +ALSILW+V KLAPEEC+ LAVE GLAAKLLLVIQSGC+  
Sbjct: 361 NTIPYTVRVLMRVSENCTNYALSILWSVCKLAPEECSPLAVEVGLAAKLLLVIQSGCDAA 420

Query: 421 LKQRSSELLKLCSLNYPDSIFISKCKLTRTIQ 453
           LKQRS+ELLKLCSL+Y D++FISKCKLTRTIQ
Sbjct: 421 LKQRSAELLKLCSLHYSDTMFISKCKLTRTIQ 448

[Close]

BLAST of Spg025222 vs. TAIR 10
Match: AT5G65920.1 (ARM repeat superfamily protein )

HSP 1 Score: 547.0 bits (1408), Expect = 1.5e-155
Identity = 292/453 (64.46%), Postives = 356/453 (78.59%), Query Frame = 0


Query: 1   MPMYQPSTWRQGDPKLVVGCGAQILDLETVVKDGILGGGGGGGL-ISGGGAEKLDLKEMI 60
           MPM+QPS  + G      G    ILDL + VKDG+LGGG G  L +     +KLDLKEMI
Sbjct: 1   MPMFQPS--KNG------GFDGHILDLHSAVKDGVLGGGDGKFLVVVTDEKKKLDLKEMI 60

Query: 61  EELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDD 120
            ELE   E+PSVFICPISLEPMQDPVTLC+GQTYERSNILKWF++GH TCPTTMQELWDD
Sbjct: 61  SELELP-EIPSVFICPISLEPMQDPVTLCTGQTYERSNILKWFNIGHCTCPTTMQELWDD 120

Query: 121 SVTPNRTLHQLIHTWFLQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHVV 180
            VTPN+TLHQLI+TWF QKY+ +KKRSEDVQGR +E+L +L+K KG+A+V +L +L+ VV
Sbjct: 121 LVTPNKTLHQLIYTWFSQKYVLMKKRSEDVQGRAIEILGTLRKAKGKAKVHALSELKQVV 180

Query: 181 ATHSSAKKMVVENGGVELISSLLGSFTSHAVGSEAVGILVNLDLDSELKKNLIQPTRMSL 240
             H+ AKK VV+ GGV +ISSLL  FTSHAVGSEA+ ILVNL+LDS+ K  L+QP R+SL
Sbjct: 181 MAHAIAKKTVVDEGGVFVISSLLSPFTSHAVGSEAIAILVNLELDSDSKAGLMQPARVSL 240

Query: 241 IADILNEGSIETKINCTKFIKMLIEGDDLRTENVSSLSLLVGLLRMVKDQRHPNGVTAGL 300
           + D+LN+GSIETKINC + I  L+E    R E VSS SLLVGL+R+VKD+R  NGV+  L
Sbjct: 241 MVDMLNDGSIETKINCARLIGRLVEEKGFRAELVSSHSLLVGLMRLVKDRRRRNGVSPAL 300

Query: 301 SLLRTLCSNESIRKQLVSIGAVAKLGGALSGLNVDCLESALYILDVLSSLPEGRLALKDC 360
           +LL+++  ++ +R  LV IGAV +L   L  L+V+CLESAL++LD L    EGR+ALKD 
Sbjct: 301 TLLKSVSVHKQVRNLLVRIGAVPQLVDVLPCLDVECLESALFVLDSLCLESEGRIALKDS 360

Query: 361 EDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQSGCNK 420
            +TI + V+LLMKVSE CT +A+SILW+V KLA EEC+ LAVE GLAAKLLLVIQSGC+ 
Sbjct: 361 VNTIPHTVRLLMKVSEKCTNYAISILWSVCKLASEECSSLAVEVGLAAKLLLVIQSGCDP 420

Query: 421 ELKQRSSELLKLCSLNYPDSIFISKCKLTRTIQ 453
            LKQRS+ELLKLCSL+Y DS+FISKCKLTRTIQ
Sbjct: 421 ALKQRSAELLKLCSLHYSDSMFISKCKLTRTIQ 444

[Close]

BLAST of Spg025222 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 158.7 bits (400), Expect = 1.1e-38
Identity = 126/400 (31.50%), Postives = 205/400 (51.25%), Query Frame = 0


Query: 59  IEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLG-HLTCPTTMQELW 118
           IE L+  I++P  F CPISLE MQDPVT+C+GQTY+R++I  W S+G + TCP T   L 
Sbjct: 5   IEPLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS 64

Query: 119 DDSVTPNRTLHQLIHTWFLQ------KYLALKKRSEDVQGRVLELLDSLKKVKG-----Q 178
           D ++ PN TL +LI  W +       + +   K+  D    V  LL     + G     +
Sbjct: 65  DFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTS-VRALLSQASAITGTHVSVR 124

Query: 179 ARVQSLKDLRHVVATHSSAKKMVVENGGVE-LISSLLGSFTSHAVGSEAVGILVNLDL-- 238
           +R  +L+ LR         + ++  +   E LI  L    TS  + SE++ +LV L +  
Sbjct: 125 SRAAALRRLRGFARDSDKNRVLIAAHNATEILIKILFSETTSSELVSESLALLVMLPITE 184

Query: 239 DSELKKNLIQPTRMSLIADILNEGSIETKINCTKFIKMLIEGD---DLRTENVSSLSLLV 298
            ++       P R+  +  +L + SIET++N    I+++  G    DL+    +S S+  
Sbjct: 185 PNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESVFE 244

Query: 299 GLLRMVKDQ-RHPNGVTAGLSLLRTLCSNESIRKQLVSIGAVAKLGGALSGLNVDC-LES 358
           G+L ++++       +  G+  L  LCS +S R   ++ GA   L   L+     C  E 
Sbjct: 245 GVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCDTER 304

Query: 359 ALYILDVLSSLPEGRLALKDCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAV 418
           AL  +++L   PEG  A  +   T+  LVK +++VS+  T++A   L A+   A E    
Sbjct: 305 ALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALC-TAEERWRE 364

Query: 419 LAVEAGLAAKLLLVIQSGCNKELKQRSSELLKLCSLNYPD 439
            A  AG+  +LLL++QS C +  K+++ +LLKL   ++PD
Sbjct: 365 EAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSWPD 402

[Close]

BLAST of Spg025222 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 154.1 bits (388), Expect = 2.7e-37
Identity = 119/404 (29.46%), Postives = 208/404 (51.49%), Query Frame = 0


Query: 59  IEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWD 118
           +E L+  I++P  F CPISL+ M DPVT+ +GQTY+R++I  W ++G+ TCP T   L D
Sbjct: 5   LEPLDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTTCPVTRVALSD 64

Query: 119 DSVTPNRTLHQLIHTWF-------LQKYLALKKRSEDVQGRVLELLDSLKKVKG-----Q 178
            ++ PN TL +LI  W        +++    K+ ++ +   V  LL     + G     +
Sbjct: 65  FTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPIS--VRSLLSQASAITGTHVSVR 124

Query: 179 ARVQSLKDLRHVVATHSSAKKMVVENGGVELISSLL------GSFTSHAVGSEAVGILVN 238
           +R  +++ LR +       + ++  +   E++  +L       S +S  V SE++ +LV 
Sbjct: 125 SRAAAIRRLRGLARDSEKNRVLIAGHNAREILVRILFADIETTSLSSELV-SESLALLVL 184

Query: 239 LDL-DSELKKNLIQPTRMSLIADILNEGSIETKINCTKFIKMLIEGD---DLRTENVSSL 298
           L + ++E +     P+R+  +  +L + SIE ++N    I+M++ G    DL+     S 
Sbjct: 185 LHMTETECEAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSD 244

Query: 299 SLLVGLLRMVKDQ-RHPNGVTAGLSLLRTLCSNESIRKQLVSIGAVAKLGGALSGLNVDC 358
           S+  G+L ++K+       +  G+  +  LC  +  R   +S GA   L   L+     C
Sbjct: 245 SIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADFDRC 304

Query: 359 -LESALYILDVLSSLPEGRLALKDCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPE 418
             E  L  +++L  LPEG  A  +   T+  +VK +++VS+  T++A   L A+   A E
Sbjct: 305 DTERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALC-TAEE 364

Query: 419 ECAVLAVEAGLAAKLLLVIQSGCNKELKQRSSELLKLCSLNYPD 439
            C   A  AGL  +LLL++QS C +  K+++  LLKL   ++PD
Sbjct: 365 RCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDSWPD 404

[Close]

BLAST of Spg025222 vs. TAIR 10
Match: AT5G64660.1 (CYS, MET, PRO, and GLY protein 2 )

HSP 1 Score: 142.1 bits (357), Expect = 1.1e-33
Identity = 120/398 (30.15%), Postives = 202/398 (50.75%), Query Frame = 0


Query: 66  IEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDSVTPNR 125
           I VP+ F CPISL+ M+ PV+LC+G TY+R++I +W   G+ TCP TMQ L +    PNR
Sbjct: 8   ITVPTFFRCPISLDVMKSPVSLCTGVTYDRASIQRWLDGGNNTCPATMQILQNKDFIPNR 67

Query: 126 TLHQLIHTWFLQKYLALKKRSEDVQGRVL-ELLDSLKKVKGQARVQSLKDLRHVVATHSS 185
           TL +LI  W           S ++      E+ D++ +VK +   +  +D R V++    
Sbjct: 68  TLQRLIEIWSDSVRRRTCVESAELAAPTRDEIADAIDRVKIE---KEERDDREVLSKIVR 127

Query: 186 AKKMVVENGG------------VELISSLLGSFTSHA---VGSEAVGILVNLD---LDSE 245
             +   +N G            V+LI+ +    TS A   V  EAV IL  +     D  
Sbjct: 128 FGRESDDNRGFLAGKDDFVKLLVDLINQVDFETTSAAKSLVVQEAVKILSTIRSKVSDRR 187

Query: 246 LKKNLIQPT---RMSLIADILNEGSIETKINCTKFIKMLIEGDDLRTENVSSLSLLVGLL 305
              NLI      R+S+I  +   G++E KI+C   ++ +    + +        L+  L+
Sbjct: 188 RFSNLILTNGRDRLSVIVYLFKTGNVELKIDCAGLLEFIAVDAESKLLIAERDGLITELM 247

Query: 306 RMVKDQRHPNGVTAGLSLLRTLCSNESIRKQLVS---IGAVAK-LGGALSGLNVDCLESA 365
           + +      + + + LS L  + S + ++  L+    IG V K L  + S L+V   E  
Sbjct: 248 KSISKDSDLSLIESSLSCLIAISSPKRVKLNLLREKLIGDVTKLLSDSTSSLSVSVTEKC 307

Query: 366 LYILDVLSSLPEGRLALKDCE-DTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAV 425
           L +L++L+S  EGR  +   + + ++ +VK LMKVS   T+ A+++LW+V  L  E+ A+
Sbjct: 308 LKLLEILASTKEGRSEICGGDGECLKTVVKKLMKVSTAATEHAVTVLWSVSYLFKEDKAL 367

Query: 426 LAV-EAGLAAKLLLVIQSGCNKELKQRSSELLKLCSLN 436
            AV       K+LL++QS C+  +++  ++LLK+  +N
Sbjct: 368 EAVTSVNGVTKILLLLQSNCSPAVRRMLTDLLKVFKVN 402

The following BLAST results are available for this feature:

BLAST of Spg025222 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038884660.1	4.6e-236	95.13	U-box domain-containing protein 31-like [Benincasa hispida]	[more]
XP_031736908.1	4.0e-232	93.00	U-box domain-containing protein 30 isoform X2 [Cucumis sativus]	[more]
XP_011649559.1	4.0e-232	93.00	U-box domain-containing protein 30 isoform X1 [Cucumis sativus] >KAE8652062.1 hy...	[more]
XP_008444531.1	2.0e-231	93.22	PREDICTED: U-box domain-containing protein 30-like [Cucumis melo] >KAA0060963.1 ...	[more]
XP_022962495.1	3.6e-225	90.93	U-box domain-containing protein 31-like isoform X1 [Cucurbita moschata]	[more]

BLAST of Spg025222 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q058P4	1.8e-155	64.16	U-box domain-containing protein 30 OS=Arabidopsis thaliana OX=3702 GN=PUB30 PE=1...	[more]
Q9FHN9	2.0e-154	64.46	U-box domain-containing protein 31 OS=Arabidopsis thaliana OX=3702 GN=PUB31 PE=2...	[more]
Q10PI9	6.2e-135	55.65	U-box domain-containing protein 75 OS=Oryza sativa subsp. japonica OX=39947 GN=T...	[more]
Q9LT79	1.6e-37	31.50	U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2...	[more]
Q9FXA4	3.9e-36	29.46	U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2...	[more]

BLAST of Spg025222 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LKY4	1.9e-232	93.00	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_2G354100 PE...	[more]
A0A5A7UYA8	9.6e-232	93.22	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C...	[more]
A0A1S3BA20	9.6e-232	93.22	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103487822 PE=4 ...	[more]
A0A6J1HFA1	1.8e-225	90.93	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111462914...	[more]
A0A6J1K7W0	3.3e-224	90.49	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492478 P...	[more]

BLAST of Spg025222 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G49810.1	1.3e-156	64.16	ARM repeat superfamily protein	[more]
AT5G65920.1	1.5e-155	64.46	ARM repeat superfamily protein	[more]
AT3G19380.1	1.1e-38	31.50	plant U-box 25	[more]
AT1G49780.1	2.7e-37	29.46	plant U-box 26	[more]
AT5G64660.1	1.1e-33	30.15	CYS, MET, PRO, and GLY protein 2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003613	U box domain	SMART	SM00504	Ubox_2	coord: 71..134 e-value: 2.1E-30 score: 117.0
IPR003613	U box domain	PFAM	PF04564	U-box	coord: 68..138 e-value: 4.0E-16 score: 59.0
IPR003613	U box domain	PROSITE	PS51698	U_BOX	coord: 67..141 score: 40.046249
IPR011989	Armadillo-like helical	GENE3D	1.25.10.10		coord: 141..449 e-value: 2.6E-20 score: 74.4
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 67..140 e-value: 1.7E-24 score: 87.6
None	No IPR available	PANTHER	PTHR22849:SF142	U-BOX DOMAIN-CONTAINING PROTEIN 30	coord: 39..451
None	No IPR available	PANTHER	PTHR22849	WDSAM1 PROTEIN	coord: 39..451
None	No IPR available	CDD	cd16664	RING-Ubox_PUB	coord: 72..114 e-value: 1.30805E-24 score: 93.8374
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 64..138
IPR016024	Armadillo-type fold	SUPERFAMILY	48371	ARM repeat	coord: 157..439

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg025222.1	Spg025222.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0004842	ubiquitin-protein transferase activity