Spg025177 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg025177
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	alpha-dioxygenase 1-like
Location	scaffold13: 41874921 .. 41879985 (+)
RNA-Seq Expression	Spg025177
Synteny	Spg025177

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCACAGTTGGAGGAGTTGGAAAGAGAGAGAAAATGTTATCTGAGTTAATGAAACCCATTACAGCTCTTTTACTCCAACACTTAGTACATAATTAAAGATTTCCATCAACTTAGCTGACTATATTGTTTGTTGATGCAGATTGTGCAATATTTTTAAAAGTTGGGGGTTTGGTATAAATTACCAGTGGCTTTGGGGCTACTTCATTTATCAATTCGGCGACATCTTTATCAACGATATAACTTGTTCAACGTCGGCAGAATCCCCACCGGAGTTAGGTTTAAGGCCGTTGATTTTCCTTACAGAACCGCCGACGGCAGGTATAACGATCCTTTTAACGAAGCCGCTGGTGCCTATGGATCCTTCTTTGGCAGAAACATCCTCCCTCTCGATCAAACCAACAAAGTATATCTCCCTCATTTTAAAAAAGTCTTCAACATCCCGTTTGATAAATTTTAGTTTATAAACCCTACTTTTCTATCTACGCGAGTTTATAAACATCCTCCCTCTCAATCAAACCAAAAAAACAAGTGTTTGTTAACGTTTTTGTAGCTACTAAGTCCGGACCCAATGGTGGTTGCGGAAGAAGAAAGTTCATAGATACAGGGAAGCAATTCAATATGATTGCTGTTTCTTGGATTCAATTCATGATTCATGATTGGATTGATCATCTAGAGGATACCAAACAGGTCCAACATTATATATAGTGATTAATGTTCCGTTCAGTTTACTAGTGATTATATTATTATGATTTAAATAAACAAATGAGAGAATATTTGTGCTAATTAAGTAAAAAGTAAGGAAAACGAAGTGTGTATGAGTTTATTTGCAGAAGGAATATCCAGAAGTTGAAGAGGAAGAGCTATATCGGCATGCGAGGCTTGTAACTTCTGCAGTGATTGCTAAGCTTCACACCATAGATTGGAGCGTTCAGCTTACCAAACACTCATACTTTGCTTGCCGGACTCCGTGCCAATTGGTAAAAAACCCTTTTTCAACCTCTTTTTTCATTTTTCAAAAGACAAAAAAGAAAACCATATAATTCATTAATTAATAAATGGTTACTCCGAGTATAACTCAATTCTTTAAAAATATGTCATCAATCAAAAAGTTAAAGATTCGAAATTTCATCCACCATGTCATTGTGAACTAAAAAAAATCATTGATTTAATTTTAGGACGTCCAACTAAATTAATTAAAATTCTTCTAATCCTATAAATAGTTGGTTTAAAATAATTTTTCTCCAAACATTTTAAAAGCACCAATAAAATGTGTTGATTTCAGTGAGGTTGAGAGTTCTTTTGAAGTAAATTAAAAGATTGGCTAGAATTCAAACTTTTAAAACATTTAATTAATAATGTAATTGAAATAAACCACACTTGGATAGCTTTTTAATATAATATATCGTCCTTCTTTGTTAAAGGTATGGAATTTTGGGAAAGAAATTCAAGGACACATTTGGACACATCGGGGGATCCATACTCGGAGGTTTAGTGGGCTTGAAGAAACCTAATAATCACAACATCCCTTATTCCATGACCGAAGAATTTACGAGTGTTTATCGAATGCACTCCCTCTTACCTGATGATTTTCATTTAAGAGATGTTTCCATTGATCCAGATCACAATAAATCTCCACCATTAATTCACAAGTACCTCCCCCTATCCCTCAATCATTTTTGTTTTTGTTCTTGTTTCTTTTAGGTTCATTAATAGTTTTTTATATATAAAAATTGAAGTGTCATTATAAAATCGTAAATCATGTAAATTCATTATTACTAATAAAAAAGTTTTGAGTTATTTTTTTGTATTGATGATTTTTCATTTTTTAAAAAGGCCACCTATGAAAACTTTGATTTTTTTTTTTTTGGTTCGGATAGAGTGCCAATGGTAAACATGGTGGGCCTTAAGGGAGAGCAAGCATCAAAGGAAATGGGCTTCACGGCCCTGATCGTTTCCATGGGCCACCAAGCCAGTGGAGCCCTACAGCTTTGGAATTATCCGCTGTGGCTGAGGAATCTTACAGCTCATGACGTGGACGGCAAAGATAGGCCCGACCACGTTGACCTGGGTGCCCTCGATGGTTGACTGACTAGAACCCTTCATCTCTCACTTGATTTAGTTTGCACCGTTGATCTGGCGGAGTTTGATTATTGTGTTTTTGACATATACAATTTTGCAGTTTACAGAGATAGGGAGAGGAATGTTGGTAGGTATAATGGCTTCCGTAGGGGATTGCTGCTGAAACCAATCTCCAGATGGGAAGATCGGACGGATGACAATGAGGCGATTGAAGCTCTCGGTGAAGTGTACGGTGATGATGTGGAGGAGCTAGCTTGATATTCTTGTAGGTCTGATGGCAGAGAAGAAAATGAAGGGCTTCGCCATTAGTGAGACTGGTTTTATGATTTTCTTGTTGATGGCTTCCAGGTAGAGGTATTTGATTGGGTTTTCGTTTATATTAAATGATGAATAATGGAAAATTAAGAATAAGAATAAAGCTGAGAATAATGCAGGAGGATAGAGGCAGATAGATTCTTCACAAGCCATTTCAATGAAGAGACATACACAAAGAAGGGATTGGAATGGGTGAAGAGTACAGAGAGGTTGAAGGATGTGATCCTGACATCTCAAACAAATGGATAAACTCATCAACTGCTTTCTCAGTTTGGGACTCTCCTCCCAACAAACCCAACCCTATCCCAATTTACCTTCGAATTCCACATTGAATCTTTAATTTTAAACCACCCAAATGTTTTTCTTCAACCCTCTCATAAGTCATATCATCATCTTTAAATTTGGTCCTAGCTAGCTACCTATTTTCTTCTTGTTTCATAATTAAGTTTGGCCTTATTCTATTGTCATGTTTTCATTGAACAATAATACTTGGTGCTTAACCATCTACTTTTCCCTCTCTAATATCATACTCTCCCTCTCAAAGTTAACTACAACAAATTTGATTTGGGCCAGGATAAATAAATACATTTTTATTCCTTTCGGATCTTAACGTATTCATATTAAGTATATATATTTTTTTTTTTTAAAAAAAAAAAGTTGGAAATGTATTAAACATGCTGAAGTATTAACATAGAGCCATGCACTAAAATTGACATCATCACTTATTCCCCGGTGTGGATCGATATTTATACTTGATAGCCCAGAAAAGGTAGTGCAAGAAATTGAAGCCACTGAGGGCGCACATAAGCCAGTAGAAGTTATCAAGTCGATAGTGATTGATATCGTGGCCAGACAGCCAAGGCGAGTGGCCAGATTTGCCCGTAACCCGGTTCACCACCGACACAATCACCGAGCTGAGATAATACCCAACCGCCAAAGAAGCCCACGTCAGCGCCGTCGCCAAAGACCTCATGCCAGCAGGAGCCTCCGTGAAGAAAAACTCCAACAAACCCGCCAACGTGAACAGATCCGCGGAGCCCAGAAACAAATACTGAAACGCGATCCAAAGGAAGGTAATGGGCAGCGGGTGGGTGCCAGTGCCAGTGCCACTGCCCGGGTGGGCATCGGCGACGGCCTTGCGCTTGAGCTCGACGAGGGCGGCGACGGCCATGGCGAGGACGGAGAGGAGGAGGCCGACGCCGATGCGTTGGAGGTGGGTGATGCCGGACTCCGAGTGGGTGAGGGATCGGGCGAAGGGGATGATGAGGTGGTCGTAGAGAGGGGCGAGGATGATGATGAAGAGCACTGGGAACACAGGTAGAGAGGCCGGGGGGACTTTGAGGGAGCCGATCTTAGTGTTCATCGTCGAGGCTTGCTCCACGGAGAAGGTGGAGAGCTGAGCCAGGCAGGCATTCAGGATGATCGTGCAGCCGAAGATGGGGAGGACTTTCAGCACCATTTTGACGTCTTCTAGTTGTTGGGTTGTGCAGTTTAGAGATGGGTGGAAGGCTGGGTTTTGGACGGCTCTGTTCAGGAATTTGAGGCTTTGGGTTGGAGCCGTGGTGGTGATGATTTCTGGCTCGTTGTTCAGCTTTGGGGCTGCTGTGGTGGAGGATGGGCTTGTTATTGCCATGTTTGCCACTGCGTTTGTCGGTGGTTTCGTGCTGCTGCAGCGGTTGAATGTTGCTGCTAGTAATACCTGAGAGGCTGAGATTAATTAAACACGTACCGTACCGTACGACATTATATTCAAAATTGAATCTTTTTAATTAGTAAAATTGAGAATGTAGTTACCTTAAGAATGGTAGTAAGAGGGCTGCCAGTGGGGATCTTGTTACGATAAAAAGAGGATCCAGCAAGGAAGAGAGGAATAGAGAGGAAAATGCTGAGAGTGGAGATTCCAAATCCCCATTGCCAGCCCAAGTTGTCTTCAATCCAAACCACGAGAGTGACGGCAACGAGCGCTCCGCAAGAGAGGCTGAAGACGAAGTAGTTGAAGAAAGTGGACCTCCTCCTCCTCCCCTGTGGGCTGGCTTCGTCGAACTGCTCTGCCCCATGCGACGGCAGGGACCCCTTTATGCCGCCCACCCCCACCGCCACTAGGTACAGCCCAATGAACAGCACCGCCGCCTCCCTCCCCGCCACTTCCCTGCACGCCTTCGGGTTGCTCTCCTCGCATGATGGTGGCATTAGTGATGGCACCTTTGCTTGCACTGTCAGGATCACCAACCCCTTTTTCCCAATTCCATGTGCAAATGTACACAACAACAATAATCATCAGCATATAAAAACAAATAGATGGAATAGGAGAAATTATTGTGTGGTTTTATAGCCTTAATTAAATAAAAAGGATGGAAAACGACAAGTTGGGAAATTGAGTTGGTGGAGCTGAAAACGAGATATAAAGAAAAGGGAAAAGAAAGCTTTATTTGGTTTGACACAAACGGATGAGTTCCAATGTCGTAACACACACACTCGTGGATGACATTATGCATTCACGCCAATGTGGGGCCCTTCTATCTTACACTTTACAAAAAGAAACCGAGCACCAAAGTTTTTTTTCTTTCTTTCTTTTTCCTCTTTTTCTTTCATGGCTAAATATTTTATCATTTTCCTCCATATAGACTCTACGTCATGCAATTTGTAAACTTCTCCTCCTATCTTATATGGAA

mRNA sequence

ATGATTGCTGTTTCTTGGATTCAATTCATGATTCATGATTGGATTGATCATCTAGAGGATACCAAACAGAAGGAATATCCAGAAGTTGAAGAGGAAGAGCTATATCGGCATGCGAGGCTTGTAACTTCTGCAGTGATTGCTAAGCTTCACACCATAGATTGGAGCGTTCAGCTTACCAAACACTCATACTTTGCTTGCCGGACTCCGTGCCAATTGGTATGGAATTTTGGGAAAGAAATTCAAGGACACATTTGGACACATCGGGGGATCCATACTCGGAGAGTGCCAATGGTAAACATGGTGGGCCTTAAGGGAGAGCAAGCATCAAAGGAAATGGGCTTCACGGCCCTGATCGTTTCCATGGGCCACCAAGCCAGTGGAGCCCTACAGCTTTGGAATTATCCGCTGTGGCTGAGGAATCTTACAGCTCATGACGTGGACGGCAAAGATAGGCCCGACCACGTTGACCTGGGTGCCCTCGATGTTTACAGAGATAGGGAGAGGAATGTTGGTAGGTATAATGGCTTCCGTAGGGGATTGCTGCTGAAACCAATCTCCAGATGGGAAGATCGGACGGATGACAATGAGGCGATTGAAGCTCTCGGTGAAGTGTACGGTGATGATGTGGAGGAGCTAGCTTGA

Coding sequence (CDS)

ATGATTGCTGTTTCTTGGATTCAATTCATGATTCATGATTGGATTGATCATCTAGAGGATACCAAACAGAAGGAATATCCAGAAGTTGAAGAGGAAGAGCTATATCGGCATGCGAGGCTTGTAACTTCTGCAGTGATTGCTAAGCTTCACACCATAGATTGGAGCGTTCAGCTTACCAAACACTCATACTTTGCTTGCCGGACTCCGTGCCAATTGGTATGGAATTTTGGGAAAGAAATTCAAGGACACATTTGGACACATCGGGGGATCCATACTCGGAGAGTGCCAATGGTAAACATGGTGGGCCTTAAGGGAGAGCAAGCATCAAAGGAAATGGGCTTCACGGCCCTGATCGTTTCCATGGGCCACCAAGCCAGTGGAGCCCTACAGCTTTGGAATTATCCGCTGTGGCTGAGGAATCTTACAGCTCATGACGTGGACGGCAAAGATAGGCCCGACCACGTTGACCTGGGTGCCCTCGATGTTTACAGAGATAGGGAGAGGAATGTTGGTAGGTATAATGGCTTCCGTAGGGGATTGCTGCTGAAACCAATCTCCAGATGGGAAGATCGGACGGATGACAATGAGGCGATTGAAGCTCTCGGTGAAGTGTACGGTGATGATGTGGAGGAGCTAGCTTGA

Protein sequence

MIAVSWIQFMIHDWIDHLEDTKQKEYPEVEEEELYRHARLVTSAVIAKLHTIDWSVQLTKHSYFACRTPCQLVWNFGKEIQGHIWTHRGIHTRRVPMVNMVGLKGEQASKEMGFTALIVSMGHQASGALQLWNYPLWLRNLTAHDVDGKDRPDHVDLGALDVYRDRERNVGRYNGFRRGLLLKPISRWEDRTDDNEAIEALGEVYGDDVEELA

Homology

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BLAST of Spg025177 vs. NCBI nr
Match: XP_022150068.1 (alpha-dioxygenase 1-like [Momordica charantia])

HSP 1 Score: 239.2 bits (609), Expect = 3.3e-59
Identity = 132/247 (53.44%), Postives = 157/247 (63.56%), Query Frame = 0


Query: 20  DTKQKEYPEVEEEELYRHARLVTSAVIAKLHTIDWSVQLTKHSYFACRTPCQLVWNFGKE 79
           DT +KE PE+E+E+LYR+ARLVTSAVIAK+HTIDW+V+L K                GK+
Sbjct: 287 DTLKKEDPELEDEDLYRYARLVTSAVIAKIHTIDWTVELLKTDTLLAGMRGNWYGLLGKK 346

Query: 80  IQ---GHIW-------------THRGI--------------HT----------------- 139
            +   GH+               + GI              H+                 
Sbjct: 347 FKDTFGHVGGSIFGGLVGLKKPNNHGIPYSLTEEFASVYRMHSLLPDDFYLRDVTIDPHR 406

Query: 140 -------RRVPMVNMVGLKGEQASKEMGFTALIVSMGHQASGALQLWNYPLWLRNLTAHD 199
                   +VPM NM+G  GE+ASK+MGFTAL+VSMGHQASGAL+LWNYPLWLR+LT HD
Sbjct: 407 NKSPPLINKVPMGNMIGHNGEEASKKMGFTALLVSMGHQASGALELWNYPLWLRDLTPHD 466

Query: 200 VDGKDRPDHVDLGALDVYRDRERNVGRYNGFRRGLLLKPISRWEDRTDDNEAIEALGEVY 213
           VDGKDRPDHVDL AL+VYRDRER V RYN FRRGLLL PIS+WED TDD+EAIE L +VY
Sbjct: 467 VDGKDRPDHVDLAALEVYRDRERKVARYNDFRRGLLLIPISKWEDLTDDDEAIEVLRDVY 526

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BLAST of Spg025177 vs. NCBI nr
Match: KAE8021580.1 (hypothetical protein FH972_007457 [Carpinus fangiana])

HSP 1 Score: 239.2 bits (609), Expect = 3.3e-59
Identity = 120/193 (62.18%), Postives = 138/193 (71.50%), Query Frame = 0


Query: 20  DTKQKEYPEVEEEELYRHARLVTSAVIAKLHTIDWSVQLTKHSYFACRTPCQLVWNFGKE 79
           DT +KEYPE  +EELYRHARLVTSAVIAK+HTIDW+V+L K                   
Sbjct: 292 DTLKKEYPEFGDEELYRHARLVTSAVIAKIHTIDWTVELLKTDTLLA------------- 351

Query: 80  IQGHIWTHRGIHTRRVPMVNMVGLKGEQASKEMGFTALIVSMGHQASGALQLWNYPLWLR 139
                    G+    VP+ N++G+KGE+   E+GFT  +VSMGHQASGAL+LWNYP WLR
Sbjct: 352 ---------GMRANWVPLANLIGIKGEKVLAEIGFTKQLVSMGHQASGALELWNYPTWLR 411

Query: 140 NLTAHDVDGKDRPDHVDLGALDVYRDRERNVGRYNGFRRGLLLKPISRWEDRTDDNEAIE 199
           +L   DVDG DRPDHVDL AL+VYRDRERNV RYN FRR LLL PIS+W+D TDDNEAI 
Sbjct: 412 DLIPQDVDGHDRPDHVDLPALEVYRDRERNVARYNDFRRALLLIPISKWKDLTDDNEAIR 462

Query: 200 ALGEVYGDDVEEL 213
           AL EVYGDDVEEL
Sbjct: 472 ALEEVYGDDVEEL 462

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BLAST of Spg025177 vs. NCBI nr
Match: XP_023526956.1 (alpha-dioxygenase 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 237.7 bits (605), Expect = 9.5e-59
Identity = 128/247 (51.82%), Postives = 149/247 (60.32%), Query Frame = 0


Query: 20  DTKQKEYPEVEEEELYRHARLVTSAVIAKLHTIDWSVQLTKHSYFAC------------- 79
           D  +KE P++E+E+LYRHARLVTSAVIAK+HTIDW+V+L K                   
Sbjct: 292 DALKKENPDLEDEDLYRHARLVTSAVIAKVHTIDWTVELLKTDTLLAGMRGNWYGLLGKK 351

Query: 80  -------------------------RTPCQLVWNFGKEIQGHIWTHRGIHTR-------- 139
                                      P  L   F    + H       H R        
Sbjct: 352 FKDTFGHVGGAILGGLVGLKKPDNHGVPYSLTEEFASVYRMHSLLPDDFHCRDVSVEPDH 411

Query: 140 --------RVPMVNMVGLKGEQASKEMGFTALIVSMGHQASGALQLWNYPLWLRNLTAHD 199
                    VPM NM+GLKGEQASKEMGFTAL+VSMGHQ+SGAL+LWNYP+WLR+L AHD
Sbjct: 412 NKSPPLIETVPMGNMIGLKGEQASKEMGFTALLVSMGHQSSGALELWNYPVWLRDLIAHD 471

Query: 200 VDGKDRPDHVDLGALDVYRDRERNVGRYNGFRRGLLLKPISRWEDRTDDNEAIEALGEVY 213
           +DGKDRPDHVDL AL++YRDRER V RYN FRRGL L PIS+WED TDD EAIE L +VY
Sbjct: 472 MDGKDRPDHVDLAALEIYRDRERKVARYNDFRRGLFLIPISKWEDLTDDKEAIEVLRDVY 531

[Close]

BLAST of Spg025177 vs. NCBI nr
Match: XP_042961862.1 (alpha-dioxygenase 2-like isoform X4 [Carya illinoinensis])

HSP 1 Score: 237.3 bits (604), Expect = 1.2e-58
Identity = 124/268 (46.27%), Postives = 158/268 (58.96%), Query Frame = 0


Query: 1   MIAVSWIQFMIHDWIDHLEDTKQKEYPEVEEEELYRHARLVTSAVIAKLHTIDWSVQLTK 60
           MIA SWIQFMIHDWIDHLEDT+Q  Y ++++E+LYRHARLVTSAVIAK+HTIDW+V+L +
Sbjct: 149 MIACSWIQFMIHDWIDHLEDTEQVHYSDLDDEQLYRHARLVTSAVIAKIHTIDWTVELLR 208

Query: 61  HSYFACRTPCQLVWNFGKEIQ---GHI----------------------WTHRGIHTRR- 120
                           GK+ +   GHI                       T   +   R 
Sbjct: 209 TDTLLAGMRINWYGFLGKKFKDTFGHILGPILSGLVGLRKPIDHGVPYSLTEEFVSVYRM 268

Query: 121 ------------------------------VPMVNMVGLKGEQASKEMGFTALIVSMGHQ 180
                                         VPM  +VG +GE+    +G   ++VSMGHQ
Sbjct: 269 HSLLPDKFFLRDITYSSTSDQDKCPPVLEEVPMRELVGTEGERKLSMIGMEQMLVSMGHQ 328

Query: 181 ASGALQLWNYPLWLRNLTAHDVDGKDRPDHVDLGALDVYRDRERNVGRYNGFRRGLLLKP 213
           A GA+ LWNYP W+RNL AHD++G+DRPD VD+  +++YRDRER V RYN FRR LL+ P
Sbjct: 329 ACGAITLWNYPSWMRNLVAHDINGEDRPDQVDMATMEIYRDRERGVARYNEFRRNLLMIP 388

[Close]

BLAST of Spg025177 vs. NCBI nr
Match: KAF3965926.1 (hypothetical protein CMV_009930 [Castanea mollissima])

HSP 1 Score: 236.9 bits (603), Expect = 1.6e-58
Identity = 121/266 (45.49%), Postives = 160/266 (60.15%), Query Frame = 0


Query: 1   MIAVSWIQFMIHDWIDHLEDTKQKEYPEVEEEELYRHARLVTSAVIAKLHTIDWSVQLTK 60
           MIA SWIQF++HDW+DHLEDT+Q  YP++++E+LYRHARLVT+AVIAK+HTIDW+V+L K
Sbjct: 87  MIACSWIQFIVHDWVDHLEDTEQVHYPDLDDEQLYRHARLVTAAVIAKIHTIDWTVELLK 146

Query: 61  HSYFACRTPCQLVWNFGKEIQ---GHI--------------------------------- 120
                           GK+ +   GH+                                 
Sbjct: 147 TDTLLVGMRVNWYGLLGKKFKDLFGHLLGPILSGLVGLKKPRDHGVPYSLTEEFLSVYRM 206

Query: 121 -------WTHRGIHT-----------RRVPMVNMVGLKGEQASKEMGFTALIVSMGHQAS 180
                  +  R + +             VPM  +VG++GE+    +G   ++VS+GHQA 
Sbjct: 207 HSLLPDTFVLRDVTSSTTEEKCPPILHEVPMWELVGIEGERKLSTIGMDQMLVSLGHQAC 266

Query: 181 GALQLWNYPLWLRNLTAHDVDGKDRPDHVDLGALDVYRDRERNVGRYNGFRRGLLLKPIS 213
           GAL LWNYP W+RNL AHD++G+DRPD VD+  L++YRDRER V RYN FRR L++ PIS
Sbjct: 267 GALTLWNYPSWMRNLVAHDINGEDRPDPVDMATLEIYRDRERGVARYNEFRRNLMMIPIS 326

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BLAST of Spg025177 vs. ExPASy Swiss-Prot
Match: Q9C9U3 (Alpha-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=DOX2 PE=2 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 3.9e-47
Identity = 98/247 (39.68%), Postives = 137/247 (55.47%), Query Frame = 0


Query: 20  DTKQKEYPEVEEEELYRHARLVTSAVIAKLHTIDWSVQLTKHSYFACRTPCQLVWNFGKE 79
           D  ++ YP+ ++E+LYR ARLVT+AVIAK+HTIDW+++L K               FGK+
Sbjct: 280 DMLKERYPDFDDEKLYRTARLVTAAVIAKVHTIDWTIELLKTDTLTAGMRINWYGFFGKK 339

Query: 80  IQGHIWTHRG------------------------------IH------------------ 139
           ++  +    G                              +H                  
Sbjct: 340 VKDMVGARFGPLFSGLVGLKKPNDHGVPYSLTEEFVSVYRMHCLLPETLILRDMNSENVD 399

Query: 140 ------TRRVPMVNMVGLKGEQASKEMGFTALIVSMGHQASGALQLWNYPLWLRNLTAHD 199
                  R +PM  ++G K  + + ++GF  L+VSMGHQ+ GAL LWNYP W+RNL A D
Sbjct: 400 KENPAIEREIPMTELIGKKAGEKASKLGFEQLLVSMGHQSCGALTLWNYPNWMRNLVAQD 459

Query: 200 VDGKDRPDHVDLGALDVYRDRERNVGRYNGFRRGLLLKPISRWEDRTDDNEAIEALGEVY 213
           +DG+DRP  +D+ AL++YRDRER V RYN FR+ LL+ PIS+WE+ TDD EAI+ L EVY
Sbjct: 460 IDGEDRPHLIDMAALEIYRDRERGVPRYNEFRKNLLMSPISKWEELTDDEEAIKVLREVY 519

[Close]

BLAST of Spg025177 vs. ExPASy Swiss-Prot
Match: Q9SGH6 (Alpha-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=DOX1 PE=1 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 1.9e-46
Identity = 102/247 (41.30%), Postives = 137/247 (55.47%), Query Frame = 0


Query: 20  DTKQKEYPEVEEEELYRHARLVTSAVIAKLHTIDWSVQLTKHSYFAC------------- 79
           D  + E  ++E+E+LYR+ARLVTSAV+AK+HTIDW+VQL K                   
Sbjct: 288 DALKDEDDDLEDEDLYRYARLVTSAVVAKIHTIDWTVQLLKTDTLLAGMRANWYGLLGKK 347

Query: 80  -------------------------RTPCQLVWNFGKEIQGHIWTHRGIH---------- 139
                                      P  L  +F    + H      +H          
Sbjct: 348 FKDSFGHAGSSILGGVVGMKKPQNHGVPYSLTEDFTSVYRMHSLLPDQLHILDIDDVPGT 407

Query: 140 ------TRRVPMVNMVGLKGEQASKEMGFTALIVSMGHQASGALQLWNYPLWLRNLTAHD 199
                  + + M +++G KGE+    +GFT L+VSMGHQASGAL+L NYP+WLR++  HD
Sbjct: 408 NKSLPLIQEISMRDLIGRKGEETMSHIGFTKLMVSMGHQASGALELMNYPMWLRDIVPHD 467

Query: 200 VDGKDRPDHVDLGALDVYRDRERNVGRYNGFRRGLLLKPISRWEDRTDDNEAIEALGEVY 213
            +G+ RPDHVDL AL++YRDRER+V RYN FRR + + PI++WED T+D EAIE L +VY
Sbjct: 468 PNGQARPDHVDLAALEIYRDRERSVPRYNEFRRSMFMIPITKWEDLTEDEEAIEVLDDVY 527

[Close]

BLAST of Spg025177 vs. ExPASy TrEMBL
Match: A0A5N6QX97 (Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_007457 PE=4 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 1.6e-59
Identity = 120/193 (62.18%), Postives = 138/193 (71.50%), Query Frame = 0


Query: 20  DTKQKEYPEVEEEELYRHARLVTSAVIAKLHTIDWSVQLTKHSYFACRTPCQLVWNFGKE 79
           DT +KEYPE  +EELYRHARLVTSAVIAK+HTIDW+V+L K                   
Sbjct: 292 DTLKKEYPEFGDEELYRHARLVTSAVIAKIHTIDWTVELLKTDTLLA------------- 351

Query: 80  IQGHIWTHRGIHTRRVPMVNMVGLKGEQASKEMGFTALIVSMGHQASGALQLWNYPLWLR 139
                    G+    VP+ N++G+KGE+   E+GFT  +VSMGHQASGAL+LWNYP WLR
Sbjct: 352 ---------GMRANWVPLANLIGIKGEKVLAEIGFTKQLVSMGHQASGALELWNYPTWLR 411

Query: 140 NLTAHDVDGKDRPDHVDLGALDVYRDRERNVGRYNGFRRGLLLKPISRWEDRTDDNEAIE 199
           +L   DVDG DRPDHVDL AL+VYRDRERNV RYN FRR LLL PIS+W+D TDDNEAI 
Sbjct: 412 DLIPQDVDGHDRPDHVDLPALEVYRDRERNVARYNDFRRALLLIPISKWKDLTDDNEAIR 462

Query: 200 ALGEVYGDDVEEL 213
           AL EVYGDDVEEL
Sbjct: 472 ALEEVYGDDVEEL 462

[Close]

BLAST of Spg025177 vs. ExPASy TrEMBL
Match: A0A6J1D8G8 (alpha-dioxygenase 1-like OS=Momordica charantia OX=3673 GN=LOC111018335 PE=4 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 1.6e-59
Identity = 132/247 (53.44%), Postives = 157/247 (63.56%), Query Frame = 0


Query: 20  DTKQKEYPEVEEEELYRHARLVTSAVIAKLHTIDWSVQLTKHSYFACRTPCQLVWNFGKE 79
           DT +KE PE+E+E+LYR+ARLVTSAVIAK+HTIDW+V+L K                GK+
Sbjct: 287 DTLKKEDPELEDEDLYRYARLVTSAVIAKIHTIDWTVELLKTDTLLAGMRGNWYGLLGKK 346

Query: 80  IQ---GHIW-------------THRGI--------------HT----------------- 139
            +   GH+               + GI              H+                 
Sbjct: 347 FKDTFGHVGGSIFGGLVGLKKPNNHGIPYSLTEEFASVYRMHSLLPDDFYLRDVTIDPHR 406

Query: 140 -------RRVPMVNMVGLKGEQASKEMGFTALIVSMGHQASGALQLWNYPLWLRNLTAHD 199
                   +VPM NM+G  GE+ASK+MGFTAL+VSMGHQASGAL+LWNYPLWLR+LT HD
Sbjct: 407 NKSPPLINKVPMGNMIGHNGEEASKKMGFTALLVSMGHQASGALELWNYPLWLRDLTPHD 466

Query: 200 VDGKDRPDHVDLGALDVYRDRERNVGRYNGFRRGLLLKPISRWEDRTDDNEAIEALGEVY 213
           VDGKDRPDHVDL AL+VYRDRER V RYN FRRGLLL PIS+WED TDD+EAIE L +VY
Sbjct: 467 VDGKDRPDHVDLAALEVYRDRERKVARYNDFRRGLLLIPISKWEDLTDDDEAIEVLRDVY 526

[Close]

BLAST of Spg025177 vs. ExPASy TrEMBL
Match: A0A6J1F353 (alpha-dioxygenase 1 OS=Cucurbita moschata OX=3662 GN=LOC111441943 PE=4 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 1.3e-58
Identity = 127/247 (51.42%), Postives = 149/247 (60.32%), Query Frame = 0


Query: 20  DTKQKEYPEVEEEELYRHARLVTSAVIAKLHTIDWSVQLTKHSYFAC------------- 79
           D  +KE P++E+E+LYRHARLVTSAVIAK+HTIDW+V+L K                   
Sbjct: 292 DALKKEDPDLEDEDLYRHARLVTSAVIAKVHTIDWTVELLKTDTLLAGMRGNWYGLLGKK 351

Query: 80  -------------------------RTPCQLVWNFGKEIQGHIWTHRGIHTR-------- 139
                                      P  L   F    + H       H R        
Sbjct: 352 FKDTFGHVGGAILGGLVGLKKPDNHGVPYSLTEEFTSVYRMHPLLPDDFHRRDVSVEPDH 411

Query: 140 --------RVPMVNMVGLKGEQASKEMGFTALIVSMGHQASGALQLWNYPLWLRNLTAHD 199
                    VPM NM+GLKGEQASKEMGFTAL+VSMGHQ+SGAL+LWNYP+WLR+L AHD
Sbjct: 412 NKSPPLIETVPMGNMIGLKGEQASKEMGFTALLVSMGHQSSGALELWNYPVWLRDLIAHD 471

Query: 200 VDGKDRPDHVDLGALDVYRDRERNVGRYNGFRRGLLLKPISRWEDRTDDNEAIEALGEVY 213
           +DGKDRPDHVDL AL++YRDRER + RYN FRRGL L PIS+WED TDD EAIE L +VY
Sbjct: 472 MDGKDRPDHVDLAALEIYRDRERKIARYNDFRRGLFLIPISKWEDLTDDKEAIEVLRDVY 531

[Close]

BLAST of Spg025177 vs. ExPASy TrEMBL
Match: A0A7J9CQM1 (Uncharacterized protein OS=Gossypium gossypioides OX=34282 GN=Gogos_001916 PE=4 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 2.3e-58
Identity = 138/300 (46.00%), Postives = 163/300 (54.33%), Query Frame = 0


Query: 1   MIAVSWIQFMIHDWIDHLEDTKQ---------------------------------KEYP 60
           MIA SWIQFMIHDWIDH+E+T Q                                 KEYP
Sbjct: 22  MIAASWIQFMIHDWIDHMENTNQQVELIAPKEVANKCPLSSFKFYPTKEFPTGFYDKEYP 81

Query: 61  EVEEEELYRHARLVTSAVIAKLHTIDWSVQLTKHSYFACRTPCQLVWNFGKEIQ---GHI 120
           ++ +EELYR+ARLVTSAVIAK+HTIDW+V+L K                GK+ +   GH+
Sbjct: 82  DLNDEELYRYARLVTSAVIAKIHTIDWTVELLKTDMLLAGMRANWYGLLGKKFKDTFGHV 141

Query: 121 W-------------------------------THR----GIHTR---------------- 180
                                            H+     IH R                
Sbjct: 142 GGSSLGGLVGLRKPVNHGVPYSLTEEFTSVYRLHQLLPDSIHLRNINVAPGPNKSPPLLE 201

Query: 181 RVPMVNMVGLKGEQASKEMGFTALIVSMGHQASGALQLWNYPLWLRNLTAHDVDGKDRPD 213
            VPM +++G KGE+   ++GFT   VSMGHQA GAL+LWNYP WLR+L A DVDGKDRPD
Sbjct: 202 EVPMPDLIGHKGEKTLSQIGFTRQFVSMGHQACGALELWNYPSWLRDLVAQDVDGKDRPD 261

[Close]

BLAST of Spg025177 vs. ExPASy TrEMBL
Match: A0A067LKZ8 (Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_09644 PE=4 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 1.1e-57
Identity = 138/329 (41.95%), Postives = 165/329 (50.15%), Query Frame = 0


Query: 1   MIAVSWIQFMIHDWIDHLEDTKQ------------------------------------- 60
           MIA SWIQFMIHDWIDHLEDT Q                                     
Sbjct: 157 MIAASWIQFMIHDWIDHLEDTNQIELTAPKEVASQCPLSSFKDGSAIYGSNAERLHKVRT 216

Query: 61  --------------------------KEYPEVEEEELYRHARLVTSAVIAKLHTIDWSVQ 120
                                     KEYP++ +EELYRHARLVTSAVIAK+HTIDW+V+
Sbjct: 217 FKDGKLKISENGLLLHDQDGIALSGDKEYPDLSDEELYRHARLVTSAVIAKIHTIDWTVE 276

Query: 121 LTKHSYFACRTPCQLVWNFGKEIQ---GHI------------------------------ 180
           L K                GK+ +   GH+                              
Sbjct: 277 LLKTDTLLAGMRANWYGLLGKKFKDTFGHVGGASLGGLVGLKKPENHGVPYSLTEEFVSV 336

Query: 181 ----------WTHRGIHT-----------RRVPMVNMVGLKGEQASKEMGFTALIVSMGH 213
                        R I T           + +PM N++G KGE+  +E+GFT  +VSMGH
Sbjct: 337 YRMHSLMPDHLALRDISTTPGSNKSLPLIKEIPMENLIGHKGEKVLEEIGFTKQMVSMGH 396

[Close]

BLAST of Spg025177 vs. TAIR 10
Match: AT1G73680.1 (alpha dioxygenase )

HSP 1 Score: 189.5 bits (480), Expect = 2.8e-48
Identity = 98/247 (39.68%), Postives = 137/247 (55.47%), Query Frame = 0


Query: 20  DTKQKEYPEVEEEELYRHARLVTSAVIAKLHTIDWSVQLTKHSYFACRTPCQLVWNFGKE 79
           D  ++ YP+ ++E+LYR ARLVT+AVIAK+HTIDW+++L K               FGK+
Sbjct: 280 DMLKERYPDFDDEKLYRTARLVTAAVIAKVHTIDWTIELLKTDTLTAGMRINWYGFFGKK 339

Query: 80  IQGHIWTHRG------------------------------IH------------------ 139
           ++  +    G                              +H                  
Sbjct: 340 VKDMVGARFGPLFSGLVGLKKPNDHGVPYSLTEEFVSVYRMHCLLPETLILRDMNSENVD 399

Query: 140 ------TRRVPMVNMVGLKGEQASKEMGFTALIVSMGHQASGALQLWNYPLWLRNLTAHD 199
                  R +PM  ++G K  + + ++GF  L+VSMGHQ+ GAL LWNYP W+RNL A D
Sbjct: 400 KENPAIEREIPMTELIGKKAGEKASKLGFEQLLVSMGHQSCGALTLWNYPNWMRNLVAQD 459

Query: 200 VDGKDRPDHVDLGALDVYRDRERNVGRYNGFRRGLLLKPISRWEDRTDDNEAIEALGEVY 213
           +DG+DRP  +D+ AL++YRDRER V RYN FR+ LL+ PIS+WE+ TDD EAI+ L EVY
Sbjct: 460 IDGEDRPHLIDMAALEIYRDRERGVPRYNEFRKNLLMSPISKWEELTDDEEAIKVLREVY 519

[Close]

BLAST of Spg025177 vs. TAIR 10
Match: AT1G73680.2 (alpha dioxygenase )

HSP 1 Score: 189.5 bits (480), Expect = 2.8e-48
Identity = 98/247 (39.68%), Postives = 137/247 (55.47%), Query Frame = 0


Query: 20  DTKQKEYPEVEEEELYRHARLVTSAVIAKLHTIDWSVQLTKHSYFACRTPCQLVWNFGKE 79
           D  ++ YP+ ++E+LYR ARLVT+AVIAK+HTIDW+++L K               FGK+
Sbjct: 289 DMLKERYPDFDDEKLYRTARLVTAAVIAKVHTIDWTIELLKTDTLTAGMRINWYGFFGKK 348

Query: 80  IQGHIWTHRG------------------------------IH------------------ 139
           ++  +    G                              +H                  
Sbjct: 349 VKDMVGARFGPLFSGLVGLKKPNDHGVPYSLTEEFVSVYRMHCLLPETLILRDMNSENVD 408

Query: 140 ------TRRVPMVNMVGLKGEQASKEMGFTALIVSMGHQASGALQLWNYPLWLRNLTAHD 199
                  R +PM  ++G K  + + ++GF  L+VSMGHQ+ GAL LWNYP W+RNL A D
Sbjct: 409 KENPAIEREIPMTELIGKKAGEKASKLGFEQLLVSMGHQSCGALTLWNYPNWMRNLVAQD 468

Query: 200 VDGKDRPDHVDLGALDVYRDRERNVGRYNGFRRGLLLKPISRWEDRTDDNEAIEALGEVY 213
           +DG+DRP  +D+ AL++YRDRER V RYN FR+ LL+ PIS+WE+ TDD EAI+ L EVY
Sbjct: 469 IDGEDRPHLIDMAALEIYRDRERGVPRYNEFRKNLLMSPISKWEELTDDEEAIKVLREVY 528

[Close]

BLAST of Spg025177 vs. TAIR 10
Match: AT3G01420.1 (Peroxidase superfamily protein )

HSP 1 Score: 187.2 bits (474), Expect = 1.4e-47
Identity = 102/247 (41.30%), Postives = 137/247 (55.47%), Query Frame = 0


Query: 20  DTKQKEYPEVEEEELYRHARLVTSAVIAKLHTIDWSVQLTKHSYFAC------------- 79
           D  + E  ++E+E+LYR+ARLVTSAV+AK+HTIDW+VQL K                   
Sbjct: 288 DALKDEDDDLEDEDLYRYARLVTSAVVAKIHTIDWTVQLLKTDTLLAGMRANWYGLLGKK 347

Query: 80  -------------------------RTPCQLVWNFGKEIQGHIWTHRGIH---------- 139
                                      P  L  +F    + H      +H          
Sbjct: 348 FKDSFGHAGSSILGGVVGMKKPQNHGVPYSLTEDFTSVYRMHSLLPDQLHILDIDDVPGT 407

Query: 140 ------TRRVPMVNMVGLKGEQASKEMGFTALIVSMGHQASGALQLWNYPLWLRNLTAHD 199
                  + + M +++G KGE+    +GFT L+VSMGHQASGAL+L NYP+WLR++  HD
Sbjct: 408 NKSLPLIQEISMRDLIGRKGEETMSHIGFTKLMVSMGHQASGALELMNYPMWLRDIVPHD 467

Query: 200 VDGKDRPDHVDLGALDVYRDRERNVGRYNGFRRGLLLKPISRWEDRTDDNEAIEALGEVY 213
            +G+ RPDHVDL AL++YRDRER+V RYN FRR + + PI++WED T+D EAIE L +VY
Sbjct: 468 PNGQARPDHVDLAALEIYRDRERSVPRYNEFRRSMFMIPITKWEDLTEDEEAIEVLDDVY 527

HSP 2 Score: 50.4 bits (119), Expect = 2.0e-06
Identity = 22/25 (88.00%), Postives = 22/25 (88.00%), Query Frame = 0


Query: 1   MIAVSWIQFMIHDWIDHLEDTKQKE 26
           MIA SWIQFMIHDWIDHLEDT Q E
Sbjct: 152 MIAASWIQFMIHDWIDHLEDTHQIE 176

The following BLAST results are available for this feature:

BLAST of Spg025177 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150068.1	3.3e-59	53.44	alpha-dioxygenase 1-like [Momordica charantia]	[more]
KAE8021580.1	3.3e-59	62.18	hypothetical protein FH972_007457 [Carpinus fangiana]	[more]
XP_023526956.1	9.5e-59	51.82	alpha-dioxygenase 1 [Cucurbita pepo subsp. pepo]	[more]
XP_042961862.1	1.2e-58	46.27	alpha-dioxygenase 2-like isoform X4 [Carya illinoinensis]	[more]
KAF3965926.1	1.6e-58	45.49	hypothetical protein CMV_009930 [Castanea mollissima]	[more]

BLAST of Spg025177 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C9U3	3.9e-47	39.68	Alpha-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=DOX2 PE=2 SV=1	[more]
Q9SGH6	1.9e-46	41.30	Alpha-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=DOX1 PE=1 SV=1	[more]

BLAST of Spg025177 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5N6QX97	1.6e-59	62.18	Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_007457 PE=4 SV=1	[more]
A0A6J1D8G8	1.6e-59	53.44	alpha-dioxygenase 1-like OS=Momordica charantia OX=3673 GN=LOC111018335 PE=4 SV=...	[more]
A0A6J1F353	1.3e-58	51.42	alpha-dioxygenase 1 OS=Cucurbita moschata OX=3662 GN=LOC111441943 PE=4 SV=1	[more]
A0A7J9CQM1	2.3e-58	46.00	Uncharacterized protein OS=Gossypium gossypioides OX=34282 GN=Gogos_001916 PE=4 ...	[more]
A0A067LKZ8	1.1e-57	41.95	Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_09644 PE=4 SV=1	[more]

BLAST of Spg025177 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G73680.1	2.8e-48	39.68	alpha dioxygenase	[more]
AT1G73680.2	2.8e-48	39.68	alpha dioxygenase	[more]
AT3G01420.1	1.4e-47	41.30	Peroxidase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR037120	Haem peroxidase domain superfamily, animal type	GENE3D	1.10.640.10	Haem peroxidase domain superfamily, animal type	coord: 20..212 e-value: 8.1E-31 score: 108.9
IPR019791	Haem peroxidase, animal-type	PFAM	PF03098	An_peroxidase	coord: 94..210 e-value: 1.3E-16 score: 60.3
IPR019791	Haem peroxidase, animal-type	PROSITE	PS50292	PEROXIDASE_3	coord: 1..213 score: 20.754139
None	No IPR available	PANTHER	PTHR11903:SF27	ALPHA-DIOXYGENASE 1-LIKE	coord: 92..212 coord: 1..25 coord: 20..62
None	No IPR available	PANTHER	PTHR11903	PROSTAGLANDIN G/H SYNTHASE	coord: 92..212 coord: 1..25 coord: 20..62
IPR013087	Zinc finger C2H2-type	PROSITE	PS00028	ZINC_FINGER_C2H2_1	coord: 66..87
IPR010255	Haem peroxidase superfamily	SUPERFAMILY	48113	Heme-dependent peroxidases	coord: 18..209

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg025177.1	Spg025177.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006979	response to oxidative stress
molecular_function	GO:0020037	heme binding
molecular_function	GO:0004601	peroxidase activity