Spg025082 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg025082
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Pectinesterase
Location	scaffold12: 9638380 .. 9640173 (-)
RNA-Seq Expression	Spg025082
Synteny	Spg025082

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGTCGAACGGATTTGCCAATGCCGAAGGTGACCAGAAGAAGAAAAAGATGGTCATAATAAGCGTCTCTTCTCTATTCCTCGTGGCAATGGTCGTCGCCGTTACCGTCGGCGTTAACAACACACCCCATGTAGGGAACACCGATGACGGCGGAGCCGCCGAGATTTCCACATCCACGAAAGCCATCCAAGCCATTTGTCAACCAACAGATTACAAAGAAACCTGCGAGCAGGGCCTCATGGCCGAAGCCGGTAACACAACGGACCCCAAGGAGCTCGTAAAAGCCGGCTTCAAAGTCGCCACCGCATATATCAACTCGGCGATTAAAAATTCCACCACTCTCCAGGAGCTGGCCAAAGACCCAAGAACCAACCAAGCCCTTCAGAACTGCCGCGAGCTTCTAGAATACGCCATCGACGATTTAACCCAGTCTTTCGATAGAATCGGCACCTTCGAAATGACGAAACTCGACGAGTTCGTCAACGATTTGAAGATCTGGCTGAGTGGGGCCCTCACCTACGAGCAAACTTGTTTGGACGGATTCGAAAACACAACCGGTGATGCCGGTGAGAAAATGCAGAAGTTTTTGAAGTCCGCTCAGCAAATGACGACTAACGGTCTTGCGATCGTAACTGAATTGTCTACTATTATGGCGTCAATGATGCCGGGAATGACGGGTGTTGGACGGCGGCTGTTGGACGAGGGAGTGCCGTCGTGGGTGAGCGACGGAAAGAGGAGGCTGATGCAGGCGGGACCGGGGGGGCTGAAGCCGGACGTGGTGGTGGCTCAAGATGGAAGTGGAAAATACAAGACGATCAACGCGGCTTTGGTTGATGTGCCGAAGAAGAGTAACAAGACATTTGTGATTTATGTCAAGGCTGGAATTTATAAGGAAATCGTTGTTGTTCCTAAGGCTTTGACTCATGTTACAATGTACGGGGATGGCCCAACCAAGACTGTCGTCACTGGTAGTTTGAACTTCATCGATGGCATTCAAACTTTCAAGACCGCAACCTTCTGTAAGTCCACATTCTTCAGTACTCTTTGTATTCTTTTATATATGTTGAAACCTAATGAATCCGAATTTATGTTTGAAACAGCTGTGGTCGGAAGTGATTTCTTCGCGAAAGACATGGGATTCGTGAACAGTGCAGGAGCGGCAAAGCACCAAGCCGTGGCGTTACGAGTCCAATCCGACAGATCGATCTTCTACAACTGTCAAATGGATGGGTATCAAGACACGCTCTACGCCCATGCCCACCGTCAGTTCTACCGTGACTGCTCGATCAGTGGCACCATTGACTTCGTCTTCGGCAACTCCGCCGCCATCTTCCAGAACTGCAAACTAATCGTCCGAAAGCCAATGGATAACCAACAATGCATCGTGACGGCTCAAGGGCGGATGAACCGCCGCGAACCGACCGCCCTAGTGTTCCAAAACTGCCACTTCATGGGCGACCCGGCTTACTTGCCATTCAAGGCCATCAACAAGGCTTATTTGGGGCGACCTTGGAAGGCATATTCACGAACCCTCATCATGGGTTCTCAAATCGACGATTTGATCCAGCCCGAGGGATGGTTGCCGTGGGGTGGCGATTTTGGGCTGACCACATTGTTCTACGCGGAGGTTCAGAACAAGGGACCAGGTGCCGATCTGAGCAAGAGAGTGAAGTGGCGAGGAATTAAAACTATTACGCCAGATCACGCCGCTAAATTCACTCCCAGGGTATTTATCGACGGCGATGCTTGGATTAAAGCCAAGGGAATTCCGTATGCTTCTGGAATGATGTAA

mRNA sequence

ATGTCGTCGAACGGATTTGCCAATGCCGAAGGTGACCAGAAGAAGAAAAAGATGGTCATAATAAGCGTCTCTTCTCTATTCCTCGTGGCAATGGTCGTCGCCGTTACCGTCGGCGTTAACAACACACCCCATGTAGGGAACACCGATGACGGCGGAGCCGCCGAGATTTCCACATCCACGAAAGCCATCCAAGCCATTTGTCAACCAACAGATTACAAAGAAACCTGCGAGCAGGGCCTCATGGCCGAAGCCGGTAACACAACGGACCCCAAGGAGCTCGTAAAAGCCGGCTTCAAAGTCGCCACCGCATATATCAACTCGGCGATTAAAAATTCCACCACTCTCCAGGAGCTGGCCAAAGACCCAAGAACCAACCAAGCCCTTCAGAACTGCCGCGAGCTTCTAGAATACGCCATCGACGATTTAACCCAGTCTTTCGATAGAATCGGCACCTTCGAAATGACGAAACTCGACGAGTTCGTCAACGATTTGAAGATCTGGCTGAGTGGGGCCCTCACCTACGAGCAAACTTGTTTGGACGGATTCGAAAACACAACCGGTGATGCCGGTGAGAAAATGCAGAAGTTTTTGAAGTCCGCTCAGCAAATGACGACTAACGGTCTTGCGATCGTAACTGAATTGTCTACTATTATGGCGTCAATGATGCCGGGAATGACGGGTGTTGGACGGCGGCTGTTGGACGAGGGAGTGCCGTCGTGGGTGAGCGACGGAAAGAGGAGGCTGATGCAGGCGGGACCGGGGGGGCTGAAGCCGGACGTGGTGGTGGCTCAAGATGGAAGTGGAAAATACAAGACGATCAACGCGGCTTTGGTTGATGTGCCGAAGAAGAGTAACAAGACATTTGTGATTTATGTCAAGGCTGGAATTTATAAGGAAATCGTTGTTGTTCCTAAGGCTTTGACTCATGTTACAATGTACGGGGATGGCCCAACCAAGACTGTCGTCACTGGTAGTTTGAACTTCATCGATGGCATTCAAACTTTCAAGACCGCAACCTTCTGTAAGTCCACATTCTTCAGTACTCTTTGTATTCTTTTATATATGTTGAAACCTAATGAATCCGAATTTATGTTTGAAACAGCTGTGGTCGGAAGTGATTTCTTCGCGAAAGACATGGGATTCGTGAACAGTGCAGGAGCGGCAAAGCACCAAGCCGTGGCGTTACGAGTCCAATCCGACAGATCGATCTTCTACAACTGTCAAATGGATGGGTATCAAGACACGCTCTACGCCCATGCCCACCGTCAGTTCTACCGTGACTGCTCGATCAGTGGCACCATTGACTTCGTCTTCGGCAACTCCGCCGCCATCTTCCAGAACTGCAAACTAATCGTCCGAAAGCCAATGGATAACCAACAATGCATCGTGACGGCTCAAGGGCGGATGAACCGCCGCGAACCGACCGCCCTAGTGTTCCAAAACTGCCACTTCATGGGCGACCCGGCTTACTTGCCATTCAAGGCCATCAACAAGGCTTATTTGGGGCGACCTTGGAAGGCATATTCACGAACCCTCATCATGGGTTCTCAAATCGACGATTTGATCCAGCCCGAGGGATGGTTGCCGTGGGGTGGCGATTTTGGGCTGACCACATTGTTCTACGCGGAGGTTCAGAACAAGGGACCAGGTGCCGATCTGAGCAAGAGAGTGAAGTGGCGAGGAATTAAAACTATTACGCCAGATCACGCCGCTAAATTCACTCCCAGGGTATTTATCGACGGCGATGCTTGGATTAAAGCCAAGGGAATTCCGTATGCTTCTGGAATGATGTAA

Coding sequence (CDS)

ATGTCGTCGAACGGATTTGCCAATGCCGAAGGTGACCAGAAGAAGAAAAAGATGGTCATAATAAGCGTCTCTTCTCTATTCCTCGTGGCAATGGTCGTCGCCGTTACCGTCGGCGTTAACAACACACCCCATGTAGGGAACACCGATGACGGCGGAGCCGCCGAGATTTCCACATCCACGAAAGCCATCCAAGCCATTTGTCAACCAACAGATTACAAAGAAACCTGCGAGCAGGGCCTCATGGCCGAAGCCGGTAACACAACGGACCCCAAGGAGCTCGTAAAAGCCGGCTTCAAAGTCGCCACCGCATATATCAACTCGGCGATTAAAAATTCCACCACTCTCCAGGAGCTGGCCAAAGACCCAAGAACCAACCAAGCCCTTCAGAACTGCCGCGAGCTTCTAGAATACGCCATCGACGATTTAACCCAGTCTTTCGATAGAATCGGCACCTTCGAAATGACGAAACTCGACGAGTTCGTCAACGATTTGAAGATCTGGCTGAGTGGGGCCCTCACCTACGAGCAAACTTGTTTGGACGGATTCGAAAACACAACCGGTGATGCCGGTGAGAAAATGCAGAAGTTTTTGAAGTCCGCTCAGCAAATGACGACTAACGGTCTTGCGATCGTAACTGAATTGTCTACTATTATGGCGTCAATGATGCCGGGAATGACGGGTGTTGGACGGCGGCTGTTGGACGAGGGAGTGCCGTCGTGGGTGAGCGACGGAAAGAGGAGGCTGATGCAGGCGGGACCGGGGGGGCTGAAGCCGGACGTGGTGGTGGCTCAAGATGGAAGTGGAAAATACAAGACGATCAACGCGGCTTTGGTTGATGTGCCGAAGAAGAGTAACAAGACATTTGTGATTTATGTCAAGGCTGGAATTTATAAGGAAATCGTTGTTGTTCCTAAGGCTTTGACTCATGTTACAATGTACGGGGATGGCCCAACCAAGACTGTCGTCACTGGTAGTTTGAACTTCATCGATGGCATTCAAACTTTCAAGACCGCAACCTTCTGTAAGTCCACATTCTTCAGTACTCTTTGTATTCTTTTATATATGTTGAAACCTAATGAATCCGAATTTATGTTTGAAACAGCTGTGGTCGGAAGTGATTTCTTCGCGAAAGACATGGGATTCGTGAACAGTGCAGGAGCGGCAAAGCACCAAGCCGTGGCGTTACGAGTCCAATCCGACAGATCGATCTTCTACAACTGTCAAATGGATGGGTATCAAGACACGCTCTACGCCCATGCCCACCGTCAGTTCTACCGTGACTGCTCGATCAGTGGCACCATTGACTTCGTCTTCGGCAACTCCGCCGCCATCTTCCAGAACTGCAAACTAATCGTCCGAAAGCCAATGGATAACCAACAATGCATCGTGACGGCTCAAGGGCGGATGAACCGCCGCGAACCGACCGCCCTAGTGTTCCAAAACTGCCACTTCATGGGCGACCCGGCTTACTTGCCATTCAAGGCCATCAACAAGGCTTATTTGGGGCGACCTTGGAAGGCATATTCACGAACCCTCATCATGGGTTCTCAAATCGACGATTTGATCCAGCCCGAGGGATGGTTGCCGTGGGGTGGCGATTTTGGGCTGACCACATTGTTCTACGCGGAGGTTCAGAACAAGGGACCAGGTGCCGATCTGAGCAAGAGAGTGAAGTGGCGAGGAATTAAAACTATTACGCCAGATCACGCCGCTAAATTCACTCCCAGGGTATTTATCGACGGCGATGCTTGGATTAAAGCCAAGGGAATTCCGTATGCTTCTGGAATGATGTAA

Protein sequence

MSSNGFANAEGDQKKKKMVIISVSSLFLVAMVVAVTVGVNNTPHVGNTDDGGAAEISTSTKAIQAICQPTDYKETCEQGLMAEAGNTTDPKELVKAGFKVATAYINSAIKNSTTLQELAKDPRTNQALQNCRELLEYAIDDLTQSFDRIGTFEMTKLDEFVNDLKIWLSGALTYEQTCLDGFENTTGDAGEKMQKFLKSAQQMTTNGLAIVTELSTIMASMMPGMTGVGRRLLDEGVPSWVSDGKRRLMQAGPGGLKPDVVVAQDGSGKYKTINAALVDVPKKSNKTFVIYVKAGIYKEIVVVPKALTHVTMYGDGPTKTVVTGSLNFIDGIQTFKTATFCKSTFFSTLCILLYMLKPNESEFMFETAVVGSDFFAKDMGFVNSAGAAKHQAVALRVQSDRSIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTIDFVFGNSAAIFQNCKLIVRKPMDNQQCIVTAQGRMNRREPTALVFQNCHFMGDPAYLPFKAINKAYLGRPWKAYSRTLIMGSQIDDLIQPEGWLPWGGDFGLTTLFYAEVQNKGPGADLSKRVKWRGIKTITPDHAAKFTPRVFIDGDAWIKAKGIPYASGMM

Homology

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BLAST of Spg025082 vs. NCBI nr
Match: XP_008458409.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 [Cucumis melo])

HSP 1 Score: 948.7 bits (2451), Expect = 2.3e-272
Identity = 474/603 (78.61%), Postives = 522/603 (86.57%), Query Frame = 0


Query: 3   SNGFANAEGDQKKKKMVIISVSSLFLVAMVVAVTVGVNNTPHV--GNTDDGG--AAEIST 62
           SNGFANAEGD+KKKK+ IISVSSL LVAMVVAVTVG+NN P+   G  D G     EIS+
Sbjct: 2   SNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGINNAPYEEHGEGDQGSKTTPEISS 61

Query: 63  STKAIQAICQPTDYKETCEQGLMAEAGNTTDPKELVKAGFKVATAYINSAIKNSTTLQEL 122
           STKAIQAICQPTDYKETCE+ LMAEAGNTTDPKELVK GFK+AT  +N AI+NSTTLQEL
Sbjct: 62  STKAIQAICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAIRNSTTLQEL 121

Query: 123 AKDPRTNQALQNCRELLEYAIDDLTQSFDRIGTFEMTKLDEFVNDLKIWLSGALTYEQTC 182
           +KDPRTNQALQNCRELLEYAIDDL +SF+RIGTF+M+KLDEFV DLKIWLSGALTYEQTC
Sbjct: 122 SKDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQTC 181

Query: 183 LDGFENTTGDAGEKMQKFLKSAQQMTTNGLAIVTELSTIMASM-MPGMTGVGRRLL---D 242
           LDGFENTTGDAG +MQ+FLKSAQ+MTTNGL IV ELSTI+ S+ +PGMT  GRRLL   D
Sbjct: 182 LDGFENTTGDAGTRMQEFLKSAQEMTTNGLGIVNELSTILGSLQIPGMT--GRRLLEDDD 241

Query: 243 EGVPSWVSDGKRRLMQAGPGGLKPDVVVAQDGSGKYKTINAALVDVPKKSNKTFVIYVKA 302
             +PSW+SDGKRRLMQAG  G+KPD++VAQDGSGKYKTINAAL DVP KSNKTFVIYVKA
Sbjct: 242 HEMPSWISDGKRRLMQAGAAGMKPDLIVAQDGSGKYKTINAALADVPLKSNKTFVIYVKA 301

Query: 303 GIYKEIVVVPKALTHVTMYGDGPTKTVVTGSLNFIDGIQTFKTATFCKSTFFSTLCILLY 362
           GIYKEIVV+PK +TH+TMYGDGPTKTVVTGSLNFIDGIQTFKTATF              
Sbjct: 302 GIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATF-------------- 361

Query: 363 MLKPNESEFMFETAVVGSDFFAKDMGFVNSAGAAKHQAVALRVQSDRSIFYNCQMDGYQD 422
                        + +G++F+A+DMGF N+AG+AKHQAVALRVQSDRSIFYNCQ+DGYQD
Sbjct: 362 -------------SAIGANFYARDMGFENTAGSAKHQAVALRVQSDRSIFYNCQIDGYQD 421

Query: 423 TLYAHAHRQFYRDCSISGTIDFVFGNSAAIFQNCKLIVRKPMDNQQCIVTAQGRMNRREP 482
           TLYAHAHRQFYRDC+ISGTIDFVFGN+A  FQNCKL+VRKPMDNQQCIVTA GRMNR+EP
Sbjct: 422 TLYAHAHRQFYRDCTISGTIDFVFGNAATNFQNCKLVVRKPMDNQQCIVTAHGRMNRKEP 481

Query: 483 TALVFQNCHFMGDPAYLPFKAINKAYLGRPWKAYSRTLIMGSQIDDLIQPEGWLPWGGDF 542
           TAL+FQ CHFMGDPAYLPFK+INKAYLGRPWK YSRT+I+GSQIDD+IQPEGWLPW GDF
Sbjct: 482 TALIFQGCHFMGDPAYLPFKSINKAYLGRPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDF 541

Query: 543 GLTTLFYAEVQNKGPGADLSKRVKWRGIKTITPDHAAKFTPRVFIDGDAWIKAKGIPYAS 598
           GL TLFYAEVQNKGPGA  +KRVKWRGIK ITP HAA FTPR FIDGDAWI AKGIPY+S
Sbjct: 542 GLNTLFYAEVQNKGPGAVETKRVKWRGIKHITPQHAADFTPRRFIDGDAWIPAKGIPYSS 575

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BLAST of Spg025082 vs. NCBI nr
Match: XP_004150503.1 (probable pectinesterase/pectinesterase inhibitor 21 [Cucumis sativus] >KGN47423.1 hypothetical protein Csa_021732 [Cucumis sativus])

HSP 1 Score: 940.3 bits (2429), Expect = 8.4e-270
Identity = 473/604 (78.31%), Postives = 521/604 (86.26%), Query Frame = 0


Query: 3   SNGFANAEGDQKKKKMVIISVSSLFLVAMVVAVTVGVNNTPH---VGNTDDGG--AAEIS 62
           SNGFANAEGD+KKKK+ IISVSSL LVAMVVAVTVGVNN PH    G+ + G     EI+
Sbjct: 2   SNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGEEHGDDNHGSETTPEIT 61

Query: 63  TSTKAIQAICQPTDYKETCEQGLMAEAGNTTDPKELVKAGFKVATAYINSAIKNSTTLQE 122
           +STKAIQAICQPTDYKETCE+ L AEAGNTTDPKELVK GFK+AT  +N AIKNSTTL+E
Sbjct: 62  SSTKAIQAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAIKNSTTLKE 121

Query: 123 LAKDPRTNQALQNCRELLEYAIDDLTQSFDRIGTFEMTKLDEFVNDLKIWLSGALTYEQT 182
           LAKDPRTNQALQNCRELLEYAIDDL QSF+RIGTF+M+KLD+FV DLKIWLSGALTYEQT
Sbjct: 122 LAKDPRTNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQT 181

Query: 183 CLDGFENTTGDAGEKMQKFLKSAQQMTTNGLAIVTELSTIMASM-MPGMTGVGRRLL--- 242
           CLDGFENTTGDAG +MQ+FLKSAQQMTTNGL IV ELSTI+ S+ +PGM+  GRRLL   
Sbjct: 182 CLDGFENTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQLPGMS--GRRLLEDD 241

Query: 243 DEGVPSWVSDGKRRLMQAGPGGLKPDVVVAQDGSGKYKTINAALVDVPKKSNKTFVIYVK 302
           D  +PSWVSDGKRRLMQAG   +KPD+VVAQDGSGKYKTINAAL DVP KSNKTFVI+VK
Sbjct: 242 DREMPSWVSDGKRRLMQAGAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIHVK 301

Query: 303 AGIYKEIVVVPKALTHVTMYGDGPTKTVVTGSLNFIDGIQTFKTATFCKSTFFSTLCILL 362
           AGIYKEIVV+PK +TH+TMYGDGPTKTVVTGSLNFIDGIQTFKTATF             
Sbjct: 302 AGIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATF------------- 361

Query: 363 YMLKPNESEFMFETAVVGSDFFAKDMGFVNSAGAAKHQAVALRVQSDRSIFYNCQMDGYQ 422
                         + +G++F+A+DMGF N+AGAAKHQAVALRVQSDRSIF+NCQ+DGYQ
Sbjct: 362 --------------SAIGANFYARDMGFENTAGAAKHQAVALRVQSDRSIFFNCQIDGYQ 421

Query: 423 DTLYAHAHRQFYRDCSISGTIDFVFGNSAAIFQNCKLIVRKPMDNQQCIVTAQGRMNRRE 482
           DTLYAHAHRQFYRDC+ISGTIDFVFGN+A  FQNCKL+VRKP+DNQQCIVTA GR+NR+E
Sbjct: 422 DTLYAHAHRQFYRDCTISGTIDFVFGNAATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKE 481

Query: 483 PTALVFQNCHFMGDPAYLPFKAINKAYLGRPWKAYSRTLIMGSQIDDLIQPEGWLPWGGD 542
           PTAL+FQ+CHFMGDPAYLPFKAINKAYLGRPWK YSRT+I+GS IDD+IQPEGWLPW GD
Sbjct: 482 PTALIFQSCHFMGDPAYLPFKAINKAYLGRPWKEYSRTIIIGSTIDDIIQPEGWLPWMGD 541

Query: 543 FGLTTLFYAEVQNKGPGADLSKRVKWRGIKTITPDHAAKFTPRVFIDGDAWIKAKGIPYA 598
           FGL TLFYAEVQNKG GAD SKRVKWRGIK ITP HAA FTPR FIDGDAWI AKGIPY+
Sbjct: 542 FGLNTLFYAEVQNKGAGADESKRVKWRGIKHITPQHAADFTPRRFIDGDAWIPAKGIPYS 576

[Close]

BLAST of Spg025082 vs. NCBI nr
Match: XP_038875843.1 (probable pectinesterase/pectinesterase inhibitor 21 [Benincasa hispida])

HSP 1 Score: 938.7 bits (2425), Expect = 2.4e-269
Identity = 468/601 (77.87%), Postives = 519/601 (86.36%), Query Frame = 0


Query: 3   SNGFANAEGDQKKKKMVIISVSSLFLVAMVVAVTVGVNNTP--HVGNTDDGGAAEISTST 62
           SNGFANAEGD+KKKKM IISVSSL LVAMVVAVTVGVNN P    G+   G   EIS++T
Sbjct: 2   SNGFANAEGDKKKKKMAIISVSSLILVAMVVAVTVGVNNAPKEEHGDGHGGETHEISSTT 61

Query: 63  KAIQAICQPTDYKETCEQGLMAEAGNTTDPKELVKAGFKVATAYINSAIKNSTTLQELAK 122
           KAIQAICQPTDYK+ CE+ LMAEAGNTTDPKEL K G ++AT  +N AI+NSTTLQELAK
Sbjct: 62  KAIQAICQPTDYKDVCEKNLMAEAGNTTDPKELAKVGLRIATRNLNEAIRNSTTLQELAK 121

Query: 123 DPRTNQALQNCRELLEYAIDDLTQSFDRIGTFEMTKLDEFVNDLKIWLSGALTYEQTCLD 182
           DPRTNQALQNCRELLEYAIDDL +SF+RIGTF+M+KLDEFV DLKIWLSGALTYEQTCLD
Sbjct: 122 DPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQTCLD 181

Query: 183 GFENTTGDAGEKMQKFLKSAQQMTTNGLAIVTELSTIMASM-MPGMTGVGRRLLDEG--- 242
           GFENTTGDAG +MQ+FLKS+QQMTTNGLAIV ELS ++ S+ +PGMT  GRRLL++    
Sbjct: 182 GFENTTGDAGVRMQEFLKSSQQMTTNGLAIVNELSGMLGSLQIPGMT--GRRLLEDDADE 241

Query: 243 VPSWVSDGKRRLMQAGPGGLKPDVVVAQDGSGKYKTINAALVDVPKKSNKTFVIYVKAGI 302
           +PSWVS+GKRRLMQA    +KPD+VVAQDGSGKYKTINAAL DVP KSNKTFVIYVKAGI
Sbjct: 242 MPSWVSEGKRRLMQAAAASMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIYVKAGI 301

Query: 303 YKEIVVVPKALTHVTMYGDGPTKTVVTGSLNFIDGIQTFKTATFCKSTFFSTLCILLYML 362
           YKEIVV+PKA+TH+TMYGDGPTKT+VTGSLNFIDGIQTFKTATF                
Sbjct: 302 YKEIVVIPKAMTHLTMYGDGPTKTIVTGSLNFIDGIQTFKTATF---------------- 361

Query: 363 KPNESEFMFETAVVGSDFFAKDMGFVNSAGAAKHQAVALRVQSDRSIFYNCQMDGYQDTL 422
                      A +G++F+A+DMGF N+AGAAKHQAVALRVQSDRSIFYNC++DGYQDTL
Sbjct: 362 -----------AAIGANFYARDMGFENTAGAAKHQAVALRVQSDRSIFYNCKIDGYQDTL 421

Query: 423 YAHAHRQFYRDCSISGTIDFVFGNSAAIFQNCKLIVRKPMDNQQCIVTAQGRMNRREPTA 482
           YAHAHRQFYRDC ISGTIDFVFGN+A IFQNCKL+VRKPMDNQQCIVTA GR+NR+EPTA
Sbjct: 422 YAHAHRQFYRDCIISGTIDFVFGNAATIFQNCKLVVRKPMDNQQCIVTAHGRLNRKEPTA 481

Query: 483 LVFQNCHFMGDPAYLPFKAINKAYLGRPWKAYSRTLIMGSQIDDLIQPEGWLPWGGDFGL 542
           L+FQ+CHFMGDPAYLPFK INKAYLGRPWK YSRT+I+GSQIDD+IQPEGWLPW GDFGL
Sbjct: 482 LIFQSCHFMGDPAYLPFKNINKAYLGRPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGL 541

Query: 543 TTLFYAEVQNKGPGADLSKRVKWRGIKTITPDHAAKFTPRVFIDGDAWIKAKGIPYASGM 598
            TLFYAEVQNKGPGAD SKRVKWRGI+ ITP HAA +TPR FIDGDAWI AKGIPY+SGM
Sbjct: 542 NTLFYAEVQNKGPGADESKRVKWRGIRHITPQHAADYTPRRFIDGDAWIPAKGIPYSSGM 573

[Close]

BLAST of Spg025082 vs. NCBI nr
Match: XP_023549205.1 (probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 902.9 bits (2332), Expect = 1.5e-258
Identity = 448/608 (73.68%), Postives = 504/608 (82.89%), Query Frame = 0


Query: 3   SNGFANAEGDQKKKKMVIISVSSLFLVAMVVAVTVGVNNTPHVGNTDDGGAA-------- 62
           SNGF +AEGD+KKKK+ +ISVSSL LVAMVVAVTVGVNN P     D GG          
Sbjct: 2   SNGF-DAEGDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDHG 61

Query: 63  ----EISTSTKAIQAICQPTDYKETCEQGLMAEAGNTTDPKELVKAGFKVATAYINSAIK 122
               ++S+STKAI+ ICQPTDYKETCE+ LM EAGNTTDPKELVKAGFK+A   +N+AI+
Sbjct: 62  DDGEKVSSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAIR 121

Query: 123 NSTTLQELAKDPRTNQALQNCRELLEYAIDDLTQSFDRIGTFEMTKLDEFVNDLKIWLSG 182
           NSTTLQELA+DPR NQALQNCRELLEYAIDDL  S+ RIGTF ++K +EFVNDLKIWLS 
Sbjct: 122 NSTTLQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLSA 181

Query: 183 ALTYEQTCLDGFENTTGDAGEKMQKFLKSAQQMTTNGLAIVTELSTIMASM-MPGMTGVG 242
           ALTYEQTCLDGFENTTGD+GE+M++FLKSAQ+MTTNGL IV+ELS I+ ++  P  T  G
Sbjct: 182 ALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSNILGTLHAPAAT--G 241

Query: 243 RRLLDEGVPSWVSDGKRRLMQAGPGGLKPDVVVAQDGSGKYKTINAALVDVPKKSNKTFV 302
           RRLLD  +P WVSDGKRRLMQ G G +KPD+VVAQDGSGKYKTIN ALVDVP KSNKTFV
Sbjct: 242 RRLLDTDMPLWVSDGKRRLMQMGTGAIKPDLVVAQDGSGKYKTINEALVDVPMKSNKTFV 301

Query: 303 IYVKAGIYKEIVVVPKALTHVTMYGDGPTKTVVTGSLNFIDGIQTFKTATFCKSTFFSTL 362
           IYVKAGIYKEIVV+PK +TH+TMYGDGPTK+VVTG+LNF+DGIQTFKTATF         
Sbjct: 302 IYVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATF--------- 361

Query: 363 CILLYMLKPNESEFMFETAVVGSDFFAKDMGFVNSAGAAKHQAVALRVQSDRSIFYNCQM 422
                             A +G++FF +DMGFVN+AG  KHQAVALRVQSDR+I YNCQ+
Sbjct: 362 ------------------AAIGANFFVRDMGFVNTAGPTKHQAVALRVQSDRAIIYNCQI 421

Query: 423 DGYQDTLYAHAHRQFYRDCSISGTIDFVFGNSAAIFQNCKLIVRKPMDNQQCIVTAQGRM 482
           DGYQDTLYAHA+RQFYRDC ISGTIDFVFGNSAAIFQNCKL+VRKP+DNQQCIVTA GR+
Sbjct: 422 DGYQDTLYAHANRQFYRDCIISGTIDFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRL 481

Query: 483 NRREPTALVFQNCHFMGDPAYLPFKAINKAYLGRPWKAYSRTLIMGSQIDDLIQPEGWLP 542
           NRREPTAL+FQ CHFMGDPAYLPFK +NKAYLGRPWK YSRT+IMGSQIDDLIQPEGWLP
Sbjct: 482 NRREPTALIFQGCHFMGDPAYLPFKKVNKAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLP 541

Query: 543 WGGDFGLTTLFYAEVQNKGPGADLSKRVKWRGIKTITPDHAAKFTPRVFIDGDAWIKAKG 598
           W GDF L TLFYAEVQN+GPGAD S RVKWRGI+ ITP HAA +T R FIDGDAWI  +G
Sbjct: 542 WMGDFALNTLFYAEVQNRGPGADQSHRVKWRGIRHITPQHAAAYTARRFIDGDAWIPVRG 579

[Close]

BLAST of Spg025082 vs. NCBI nr
Match: XP_022959432.1 (probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita moschata])

HSP 1 Score: 899.0 bits (2322), Expect = 2.1e-257
Identity = 445/607 (73.31%), Postives = 501/607 (82.54%), Query Frame = 0


Query: 3   SNGFANAEGDQKKKKMVIISVSSLFLVAMVVAVTVGVNNTPHVGNTDDGG---------- 62
           SNGF +AE D+KKKK+ +ISVSSL LVAMVVAVTVGVNN P     D GG          
Sbjct: 2   SNGF-DAESDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDHG 61

Query: 63  --AAEISTSTKAIQAICQPTDYKETCEQGLMAEAGNTTDPKELVKAGFKVATAYINSAIK 122
             A +IS+STKAI+ ICQPTDYKETCE+ LM EAGNTTDPKELVKAGFK+A   +N+AI+
Sbjct: 62  DDAEKISSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAIR 121

Query: 123 NSTTLQELAKDPRTNQALQNCRELLEYAIDDLTQSFDRIGTFEMTKLDEFVNDLKIWLSG 182
           NSTTLQELA+DPR NQALQNCRELLEYAIDDL  S+ RIGTF ++K +EFVNDLKIWLS 
Sbjct: 122 NSTTLQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLSA 181

Query: 183 ALTYEQTCLDGFENTTGDAGEKMQKFLKSAQQMTTNGLAIVTELSTIMASMMPGMTGVGR 242
           ALTYEQTCLDGFENTTGD+GE+M++FLKSAQ+MTTNGL IV+ELS I+ + +      GR
Sbjct: 182 ALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSKILGT-LDAPAATGR 241

Query: 243 RLLDEGVPSWVSDGKRRLMQAGPGGLKPDVVVAQDGSGKYKTINAALVDVPKKSNKTFVI 302
           RLLD  +P WVSDGKRRLMQ G G +KPD+VVAQDGSGKYKTIN ALV VP KSNKTFVI
Sbjct: 242 RLLDTDIPLWVSDGKRRLMQVGTGAIKPDLVVAQDGSGKYKTINEALVHVPMKSNKTFVI 301

Query: 303 YVKAGIYKEIVVVPKALTHVTMYGDGPTKTVVTGSLNFIDGIQTFKTATFCKSTFFSTLC 362
           YVKAGIYKEIVV+PK +TH+TMYGDGPTK+VVTG+LNF+DGIQTFKTATF          
Sbjct: 302 YVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATF---------- 361

Query: 363 ILLYMLKPNESEFMFETAVVGSDFFAKDMGFVNSAGAAKHQAVALRVQSDRSIFYNCQMD 422
                            A +G++FF +DMGFVN+AG  KHQAVALRVQSDR+I YNCQ+D
Sbjct: 362 -----------------AAIGANFFVRDMGFVNTAGPTKHQAVALRVQSDRAIIYNCQID 421

Query: 423 GYQDTLYAHAHRQFYRDCSISGTIDFVFGNSAAIFQNCKLIVRKPMDNQQCIVTAQGRMN 482
           GYQDTLYAHA+RQFYRDC ISGTIDFVFGNSAAIFQNCKL+VRKP+DNQQCIVTA GR+N
Sbjct: 422 GYQDTLYAHANRQFYRDCIISGTIDFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLN 481

Query: 483 RREPTALVFQNCHFMGDPAYLPFKAINKAYLGRPWKAYSRTLIMGSQIDDLIQPEGWLPW 542
           RREPTAL+FQ CHFMGDP+YLPFK +NKAYLGRPWK YSRT+IMGSQIDDLIQPEGWLPW
Sbjct: 482 RREPTALIFQGCHFMGDPSYLPFKKVNKAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPW 541

Query: 543 GGDFGLTTLFYAEVQNKGPGADLSKRVKWRGIKTITPDHAAKFTPRVFIDGDAWIKAKGI 598
            GDF L TLFYAEVQN+GPGAD S RVKWRGI+ ITP HAA +T R FIDGDAWI  +G+
Sbjct: 542 MGDFALNTLFYAEVQNRGPGADQSHRVKWRGIRHITPQHAAAYTARRFIDGDAWIPVRGV 579

[Close]

BLAST of Spg025082 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 644.0 bits (1660), Expect = 1.6e-183
Identity = 313/588 (53.23%), Postives = 417/588 (70.92%), Query Frame = 0


Query: 13  QKKKKMVIISVSSLFLVAMVVAVTVGVNNTPHVGNTDDGGAAEISTSTKAIQAICQPTDY 72
           ++K++ ++I++SS+ L++MVVAVTVGV+   H G  D  G AE++ S KA++ +C PTDY
Sbjct: 10  KRKRRYIVITISSVLLISMVVAVTVGVSLNKHDG--DSKGKAEVNASVKAVKDVCAPTDY 69

Query: 73  KETCEQGLMAEAGNTTDPKELVKAGFKVATAYINSAIKNSTTLQELAKDPRTNQALQNCR 132
           ++TCE  L+    NTTDP ELVK  F V    I  A K S T+ EL KD RT  AL  C+
Sbjct: 70  RKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSRTRMALDQCK 129

Query: 133 ELLEYAIDDLTQSFDRIGTFEMTKLDEFVNDLKIWLSGALTYEQTCLDGFENTTGDAGEK 192
           EL++YA+D+L+ SF+ +G FE   LDE + +L+IWLS A+++E+TCL+GF+ T G+AGE 
Sbjct: 130 ELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGET 189

Query: 193 MQKFLKSAQQMTTNGLAIVTELSTIMASM-MPGMTGVGRRLLDEGVPSWVSDGKRRLMQA 252
           M+K LK+A ++T NGLAI++E+S  +  M +PG+    RRLL EG PSWV    R+L+QA
Sbjct: 190 MKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLN--SRRLLAEGFPSWVDQRGRKLLQA 249

Query: 253 GP--GGLKPDVVVAQDGSGKYKTINAALVDVPKKSNKTFVIYVKAGIYKEIVVVPKALTH 312
                 +KPD+VVAQDGSG+YKTIN AL  VPKK N TFV+++KAG+YKE V V K ++H
Sbjct: 250 AAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTMSH 309

Query: 313 VTMYGDGPTKTVVTGSLNFIDGIQTFKTATFCKSTFFSTLCILLYMLKPNESEFMFETAV 372
           +   GDGP KT+++G+ N+ DGI T++TAT                            A+
Sbjct: 310 LVFIGDGPDKTIISGNKNYKDGITTYRTAT---------------------------VAI 369

Query: 373 VGSDFFAKDMGFVNSAGAAKHQAVALRVQSDRSIFYNCQMDGYQDTLYAHAHRQFYRDCS 432
           VG+ F AK++GF N+AGA KHQAVA+RVQSD SIF+NC+ DGYQDTLY H+HRQF+RDC+
Sbjct: 370 VGNYFIAKNIGFENTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCT 429

Query: 433 ISGTIDFVFGNSAAIFQNCKLIVRKPMDNQQCIVTAQGRMNRREPTALVFQNCHFMGDPA 492
           ISGTIDF+FG++AA+FQNC L+VRKP+ NQ C +TA GR + RE T  VFQ C   G+P 
Sbjct: 430 ISGTIDFLFGDAAAVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPD 489

Query: 493 YLPFKAINKAYLGRPWKAYSRTLIMGSQIDDLIQPEGWLPWGGDFGLTTLFYAEVQNKGP 552
           YL  K  +KAYLGRPWK YSRT+IM + I D +QP+GW PW GDFGL TLFY+EVQN GP
Sbjct: 490 YLAVKETSKAYLGRPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGP 549

Query: 553 GADLSKRVKWRGIKTITPDHAAKFTPRVFIDGDAWIKAKGIPYASGMM 598
           G+ L+ RV W GIKT++ +   KFTP  +I GD WI  KG+PY +G++
Sbjct: 550 GSALANRVTWAGIKTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGLL 566

[Close]

BLAST of Spg025082 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 9.8e-181
Identity = 316/588 (53.74%), Postives = 410/588 (69.73%), Query Frame = 0


Query: 10  EGDQKKKKMVIISVSSLFLVAMVVAVTVGVNNTPHVGNTDDGGAAEISTSTKAIQAICQP 69
           E  ++KK+ VIIS+SS+ L++MVVAVT+GV+    V  +D+ G  EI+TS KAI+ +C P
Sbjct: 8   EDAKRKKRYVIISISSVLLISMVVAVTIGVS----VNKSDNAGDEEITTSVKAIKDVCAP 67

Query: 70  TDYKETCEQGLMAEAGNTTDPKELVKAGFKVATAYINSAIKNSTTLQELAKDPRTNQALQ 129
           TDYKETCE  L  +A +T+DP ELVK  F      I+   K S T+ EL KDPR   AL 
Sbjct: 68  TDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPRAKMALD 127

Query: 130 NCRELLEYAIDDLTQSFDRIGTFEMTKLDEFVNDLKIWLSGALTYEQTCLDGFENTTGDA 189
            C+EL++YAI +L++SF+ +G FE  K+DE +  L+IWLS  +++EQTCLDGF+ T G+A
Sbjct: 128 QCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNA 187

Query: 190 GEKMQKFLKSAQQMTTNGLAIVTELSTIMASM-MPGMTGVGRRLLDEGVPSWVSDGKRRL 249
           GE ++K LK+A Q+T NGLA+VTE+S  +  M +P M    RRLL +  PSW+    RRL
Sbjct: 188 GETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMN--SRRLLSQEFPSWMDARARRL 247

Query: 250 MQAGPGGLKPDVVVAQDGSGKYKTINAALVDVPKKSNKTFVIYVKAGIYKEIVVVPKALT 309
           + A    +KPD+VVAQDGSG+YKTIN AL  VPKK N TFV+++K GIYKE V V +++T
Sbjct: 248 LNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSMT 307

Query: 310 HVTMYGDGPTKTVVTGSLNFIDGIQTFKTATFCKSTFFSTLCILLYMLKPNESEFMFETA 369
           H+   GDGP KTV++GS ++ DGI T+KTAT                            A
Sbjct: 308 HLVFIGDGPDKTVISGSKSYKDGITTYKTAT---------------------------VA 367

Query: 370 VVGSDFFAKDMGFVNSAGAAKHQAVALRVQSDRSIFYNCQMDGYQDTLYAHAHRQFYRDC 429
           +VG  F AK++ F N+AGA KHQAVA+RV +D SIFYNC+ DGYQDTLYAH+HRQFYRDC
Sbjct: 368 IVGDHFIAKNIAFENTAGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDC 427

Query: 430 SISGTIDFVFGNSAAIFQNCKLIVRKPMDNQQCIVTAQGRMNRREPTALVFQNCHFMGDP 489
           +ISGTIDF+FG++AA+FQNC L+VRKP+ NQ C +TA GR + RE T  V Q C  +G+P
Sbjct: 428 TISGTIDFLFGDAAAVFQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEP 487

Query: 490 AYLPFKAINKAYLGRPWKAYSRTLIMGSQIDDLIQPEGWLPWGGDFGLTTLFYAEVQNKG 549
            YL  K  +K YLGRPWK YSRT+IM + I D + PEGW PW G+FGL TLFY+EVQN G
Sbjct: 488 DYLAVKEQSKTYLGRPWKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTG 547

Query: 550 PGADLSKRVKWRGIKTITPDHAAKFTPRVFIDGDAWIKAKGIPYASGM 597
           PGA ++KRV W GIK ++ +   KFTP  +I GDAWI  KG+PY  G+
Sbjct: 548 PGAAITKRVTWPGIKKLSDEEILKFTPAQYIQGDAWIPGKGVPYILGL 562

[Close]

BLAST of Spg025082 vs. ExPASy Swiss-Prot
Match: Q9FJ21 (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3702 GN=PME58 PE=2 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 3.4e-165
Identity = 286/593 (48.23%), Postives = 398/593 (67.12%), Query Frame = 0


Query: 10  EGDQKKKKMVIISVSSLFLVAMVVAVTVGVNNTPHVGNTDDGGAAEISTSTKAIQAICQP 69
           +G+ KKKK +I  V +  LV MVVA  VG+  + +  +++     +I T+T A++A+C P
Sbjct: 4   DGELKKKKCIIAGVITALLVLMVVA--VGITTSRNTSHSEKIVPVQIKTATTAVEAVCAP 63

Query: 70  TDYKETCEQGLMAEAGNTTDPKELVKAGFKVATAYINSAIKNSTT--LQELAKDPRTNQA 129
           TDYKETC   LM  + ++T P +L+K GF V    I  +IK ++     + A D  T  A
Sbjct: 64  TDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGA 123

Query: 130 LQNCRELLEYAIDDLTQSFDRIGTFEMTKLDEFVNDLKIWLSGALTYEQTCLDGFENTTG 189
           L+ C +L+  A DDL +  D    F + ++++FV DL++WLSG++ Y+QTC+D FE T  
Sbjct: 124 LELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNS 183

Query: 190 DAGEKMQKFLKSAQQMTTNGLAIVTELSTIMASM-MPGMTG----VGRRLL--DEGVPSW 249
              + MQK  K+++++T+NGLA++T +S ++    + G+TG      R+LL  ++G+PSW
Sbjct: 184 KLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSAEDGIPSW 243

Query: 250 VSDGKRRLMQAGPGGLKPDVVVAQDGSGKYKTINAALVDVPKKSNKTFVIYVKAGIYKEI 309
           V    RRLM A  GG+K +VVVA DGSG+YKTIN AL  VPK + K FVIY+K G+Y E 
Sbjct: 244 VGPNTRRLM-ATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVYNEK 303

Query: 310 VVVPKALTHVTMYGDGPTKTVVTGSLNFIDG-IQTFKTATFCKSTFFSTLCILLYMLKPN 369
           V V K +THVT  GDGPTKT +TGSLN+  G ++T+ TAT                    
Sbjct: 304 VDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTAT-------------------- 363

Query: 370 ESEFMFETAVVGSDFFAKDMGFVNSAGAAKHQAVALRVQSDRSIFYNCQMDGYQDTLYAH 429
                   A+ G +F AK++GF N+AG   HQAVALRV +D ++FYNCQ+DGYQDTLY H
Sbjct: 364 -------VAINGDNFTAKNIGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVH 423

Query: 430 AHRQFYRDCSISGTIDFVFGNSAAIFQNCKLIVRKPMDNQQCIVTAQGRMNRREPTALVF 489
           +HRQF+RDC++SGT+DF+FG+   + QNC ++VRKPM +Q C++TAQGR ++RE T LV 
Sbjct: 424 SHRQFFRDCTVSGTVDFIFGDGIVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVL 483

Query: 490 QNCHFMGDPAYLPFKAINKAYLGRPWKAYSRTLIMGSQIDDLIQPEGWLPWGGDFGLTTL 549
           QNCH  G+PAY+P K+INKAYLGRPWK +SRT+IMG+ IDD+I P GWLPW GDF L TL
Sbjct: 484 QNCHITGEPAYIPVKSINKAYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTL 543

Query: 550 FYAEVQNKGPGADLSKRVKWRGIKTITPDHAAKFTPRVFIDGDAWIKAKGIPY 593
           +YAE +N GPG++ ++RVKW GIK ++P  A +FTP  F+ G+ WI    +PY
Sbjct: 544 YYAEYENNGPGSNQAQRVKWPGIKKLSPKQALRFTPARFLRGNLWIPPNRVPY 566

[Close]

BLAST of Spg025082 vs. ExPASy Swiss-Prot
Match: Q8GXA1 (Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3702 GN=PME23 PE=2 SV=3)

HSP 1 Score: 527.3 bits (1357), Expect = 2.2e-148
Identity = 275/599 (45.91%), Postives = 386/599 (64.44%), Query Frame = 0


Query: 9   AEGDQKKKKMVIISVSSLFLVAMVVAVTVGVNNTPHVGNTDDGGAAEISTSTKAIQAICQ 68
           ++GD+KKK +V  SVS  FLV MVV+V V  +   H    D+     I  +TKA+QA+C 
Sbjct: 3   SDGDKKKKFIVAGSVSG-FLVIMVVSVAVVTSK--HSPKDDEN---HIRKTTKAVQAVCA 62

Query: 69  PTDYKETCEQGLMAEAGNTTDPKELVKAGFKVATAYINSAIKNST--TLQELAKDPRTNQ 128
           PTD+K+TC   LM  + ++ DP +L+K GFKV    IN +++ ++     +  K+P    
Sbjct: 63  PTDFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKG 122

Query: 129 ALQNCRELLEYAIDDLTQSFDRIGTFEMTKLDEFVNDLKIWLSGALTYEQTCLDGFENTT 188
           A + C +L+  AIDDL +  D    F + +++ FV DL++WLSG++ ++QTC+D F    
Sbjct: 123 AFELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIK 182

Query: 189 GDAGEKMQKFLKSAQQMTTNGLAIVTELSTIMA-SMMPGMTGV----GRRLL--DEGVPS 248
            +  + M K  K+++++++N LA+VT +ST++  S + G+TG      R+LL  ++ +P+
Sbjct: 183 SNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLSTEDSIPT 242

Query: 249 WVSDGKRRLMQA---GPGGLKPDVVVAQDGSGKYKTINAALVDVPKKSNKTFVIYVKAGI 308
           WV    RRLM A   GPG +K + VVAQDG+G++KTI  AL  VPK +   F+I++K GI
Sbjct: 243 WVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGNKVPFIIHIKEGI 302

Query: 309 YKEIVVVPKALTHVTMYGDGPTKTVVTGSLNF-IDGIQTFKTATFCKSTFFSTLCILLYM 368
           YKE V V K + HVT  GDGP KT++TGSLNF I  ++TF TAT                
Sbjct: 303 YKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATI--------------- 362

Query: 369 LKPNESEFMFETAVVGSDFFAKDMGFVNSAGAAKHQAVALRVQSDRSIFYNCQMDGYQDT 428
                        + G  F AK++G  N+AG    QAVALRV +D ++F++CQ+DG+QDT
Sbjct: 363 ------------TIEGDHFTAKNIGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDT 422

Query: 429 LYAHAHRQFYRDCSISGTIDFVFGNSAAIFQNCKLIVRKPMDNQQCIVTAQGRMNRREPT 488
           LY H+HRQFYRDC++SGT+DF+FG++  I QNCK++VRKP   Q C+VTAQGR N RE T
Sbjct: 423 LYVHSHRQFYRDCTVSGTVDFIFGDAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVREST 482

Query: 489 ALVFQNCHFMGDPAYLPFKAINKAYLGRPWKAYSRTLIMGSQIDDLIQPEGWLPWGGDFG 548
            LV   CH  GDPAY+P K++NKAYLGRPWK +SRT+IM + IDD+I P GWLPW GDF 
Sbjct: 483 GLVLHGCHITGDPAYIPMKSVNKAYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFA 542

Query: 549 LTTLFYAEVQNKGPGADLSKRVKWRGIKTITPDHAAKFTPRVFIDGDAWIKAKGIPYAS 595
           L TL+YAE  N GPG++ ++RVKW GIK +TP  A  +T   F+ GD WI    +PY +
Sbjct: 543 LKTLYYAEHMNTGPGSNQAQRVKWPGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

[Close]

BLAST of Spg025082 vs. ExPASy Swiss-Prot
Match: Q42920 (Pectinesterase/pectinesterase inhibitor OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 2.3e-129
Identity = 237/477 (49.69%), Postives = 312/477 (65.41%), Query Frame = 0


Query: 128 LQNCRELLEYAIDDLTQSFDRIGTFEMTKLDEFVNDLKIWLSGALTYEQTCLDGFENTTG 187
           ++ C E+L+YA+D + +S   +  F+  KL E+  DLK+WL+G L+++QTCLDGF NTT 
Sbjct: 1   MEICNEVLDYAVDGIHKSVGTLDQFDFHKLSEYAFDLKVWLTGTLSHQQTCLDGFANTTT 60

Query: 188 DAGEKMQKFLKSAQQMTTNGLAIVTELSTIMASMMPGMTGVGRRLL-DEGVPSWVSDGKR 247
            AGE M K LK++ ++++N + ++  +S I+         V RRLL D+G+PSWV+DG R
Sbjct: 61  KAGETMTKVLKTSMELSSNAIDMMDAVSRILKGFDTSQYSVSRRLLSDDGIPSWVNDGHR 120

Query: 248 RLMQAGPGGLKPDVVVAQDGSGKYKTINAALVDVPKKSNKTFVIYVKAGIYKEIVVVPKA 307
           RL+ AG G ++P+ VVAQDGSG++KT+  AL  VP K+   FVI+VKAG+YKE V V K 
Sbjct: 121 RLL-AG-GNVQPNAVVAQDGSGQFKTLTDALKTVPPKNAVPFVIHVKAGVYKETVNVAKE 180

Query: 308 LTHVTMYGDGPTKTVVTGSLNFIDGIQTFKTATFCKSTFFSTLCILLYMLKPNESEFMFE 367
           + +VT+ GDGPTKT  TGSLN+ DGI T+ TATF                          
Sbjct: 181 MNYVTVIGDGPTKTKFTGSLNYADGINTYNTATF-------------------------- 240

Query: 368 TAVVGSDFFAKDMGFVNSAGAAKHQAVALRVQSDRSIFYNCQMDGYQDTLYAHAHRQFYR 427
             V G++F AKD+GF N+AG  KHQAVALRV +D++IFYNCQMDG+QDTLY  + RQFYR
Sbjct: 241 -GVNGANFMAKDIGFENTAGTGKHQAVALRVTADQAIFYNCQMDGFQDTLYVQSQRQFYR 300

Query: 428 DCSISGTIDFVFGNSAAIFQNCKLIVRKPMDNQQCIVTAQGRMNRREPTALVFQNCHFMG 487
           DCSISGTIDFVFG    +FQNCKL+ R P   QQC+VTA GR  +   +ALVFQ+ HF G
Sbjct: 301 DCSISGTIDFVFGERFGVFQNCKLVCRLPAKGQQCLVTAGGREKQNSASALVFQSSHFTG 360

Query: 488 DPAYLPFKAINKAYLGRPWKAYSRTLIMGSQIDDLIQPEGWLPWGGDFGLTTLFYAEVQN 547
           +PA L       +YLGRPWK YS+ +IM S ID +  PEG++PW G     T  Y E  N
Sbjct: 361 EPA-LTSVTPKVSYLGRPWKQYSKVVIMDSTIDAIFVPEGYMPWMGSAFKETCTYYEYNN 420

Query: 548 KGPGADLSKRVKWRGIKTITPDHAAKFTPRVFID------GDAWIKAKGIPYASGMM 598
           KGPGAD + RVKW G+K +T + AA++ P  F +       D WI   G+PY+ G M
Sbjct: 421 KGPGADTNLRVKWHGVKVLTSNVAAEYYPGKFFEIVNATARDTWIVKSGVPYSLGPM 447

[Close]

BLAST of Spg025082 vs. ExPASy TrEMBL
Match: A0A1S3C7B6 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497833 PE=3 SV=1)

HSP 1 Score: 948.7 bits (2451), Expect = 1.1e-272
Identity = 474/603 (78.61%), Postives = 522/603 (86.57%), Query Frame = 0


Query: 3   SNGFANAEGDQKKKKMVIISVSSLFLVAMVVAVTVGVNNTPHV--GNTDDGG--AAEIST 62
           SNGFANAEGD+KKKK+ IISVSSL LVAMVVAVTVG+NN P+   G  D G     EIS+
Sbjct: 2   SNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGINNAPYEEHGEGDQGSKTTPEISS 61

Query: 63  STKAIQAICQPTDYKETCEQGLMAEAGNTTDPKELVKAGFKVATAYINSAIKNSTTLQEL 122
           STKAIQAICQPTDYKETCE+ LMAEAGNTTDPKELVK GFK+AT  +N AI+NSTTLQEL
Sbjct: 62  STKAIQAICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAIRNSTTLQEL 121

Query: 123 AKDPRTNQALQNCRELLEYAIDDLTQSFDRIGTFEMTKLDEFVNDLKIWLSGALTYEQTC 182
           +KDPRTNQALQNCRELLEYAIDDL +SF+RIGTF+M+KLDEFV DLKIWLSGALTYEQTC
Sbjct: 122 SKDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQTC 181

Query: 183 LDGFENTTGDAGEKMQKFLKSAQQMTTNGLAIVTELSTIMASM-MPGMTGVGRRLL---D 242
           LDGFENTTGDAG +MQ+FLKSAQ+MTTNGL IV ELSTI+ S+ +PGMT  GRRLL   D
Sbjct: 182 LDGFENTTGDAGTRMQEFLKSAQEMTTNGLGIVNELSTILGSLQIPGMT--GRRLLEDDD 241

Query: 243 EGVPSWVSDGKRRLMQAGPGGLKPDVVVAQDGSGKYKTINAALVDVPKKSNKTFVIYVKA 302
             +PSW+SDGKRRLMQAG  G+KPD++VAQDGSGKYKTINAAL DVP KSNKTFVIYVKA
Sbjct: 242 HEMPSWISDGKRRLMQAGAAGMKPDLIVAQDGSGKYKTINAALADVPLKSNKTFVIYVKA 301

Query: 303 GIYKEIVVVPKALTHVTMYGDGPTKTVVTGSLNFIDGIQTFKTATFCKSTFFSTLCILLY 362
           GIYKEIVV+PK +TH+TMYGDGPTKTVVTGSLNFIDGIQTFKTATF              
Sbjct: 302 GIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATF-------------- 361

Query: 363 MLKPNESEFMFETAVVGSDFFAKDMGFVNSAGAAKHQAVALRVQSDRSIFYNCQMDGYQD 422
                        + +G++F+A+DMGF N+AG+AKHQAVALRVQSDRSIFYNCQ+DGYQD
Sbjct: 362 -------------SAIGANFYARDMGFENTAGSAKHQAVALRVQSDRSIFYNCQIDGYQD 421

Query: 423 TLYAHAHRQFYRDCSISGTIDFVFGNSAAIFQNCKLIVRKPMDNQQCIVTAQGRMNRREP 482
           TLYAHAHRQFYRDC+ISGTIDFVFGN+A  FQNCKL+VRKPMDNQQCIVTA GRMNR+EP
Sbjct: 422 TLYAHAHRQFYRDCTISGTIDFVFGNAATNFQNCKLVVRKPMDNQQCIVTAHGRMNRKEP 481

Query: 483 TALVFQNCHFMGDPAYLPFKAINKAYLGRPWKAYSRTLIMGSQIDDLIQPEGWLPWGGDF 542
           TAL+FQ CHFMGDPAYLPFK+INKAYLGRPWK YSRT+I+GSQIDD+IQPEGWLPW GDF
Sbjct: 482 TALIFQGCHFMGDPAYLPFKSINKAYLGRPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDF 541

Query: 543 GLTTLFYAEVQNKGPGADLSKRVKWRGIKTITPDHAAKFTPRVFIDGDAWIKAKGIPYAS 598
           GL TLFYAEVQNKGPGA  +KRVKWRGIK ITP HAA FTPR FIDGDAWI AKGIPY+S
Sbjct: 542 GLNTLFYAEVQNKGPGAVETKRVKWRGIKHITPQHAADFTPRRFIDGDAWIPAKGIPYSS 575

[Close]

BLAST of Spg025082 vs. ExPASy TrEMBL
Match: A0A0A0KD24 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G318700 PE=3 SV=1)

HSP 1 Score: 940.3 bits (2429), Expect = 4.0e-270
Identity = 473/604 (78.31%), Postives = 521/604 (86.26%), Query Frame = 0


Query: 3   SNGFANAEGDQKKKKMVIISVSSLFLVAMVVAVTVGVNNTPH---VGNTDDGG--AAEIS 62
           SNGFANAEGD+KKKK+ IISVSSL LVAMVVAVTVGVNN PH    G+ + G     EI+
Sbjct: 2   SNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGEEHGDDNHGSETTPEIT 61

Query: 63  TSTKAIQAICQPTDYKETCEQGLMAEAGNTTDPKELVKAGFKVATAYINSAIKNSTTLQE 122
           +STKAIQAICQPTDYKETCE+ L AEAGNTTDPKELVK GFK+AT  +N AIKNSTTL+E
Sbjct: 62  SSTKAIQAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAIKNSTTLKE 121

Query: 123 LAKDPRTNQALQNCRELLEYAIDDLTQSFDRIGTFEMTKLDEFVNDLKIWLSGALTYEQT 182
           LAKDPRTNQALQNCRELLEYAIDDL QSF+RIGTF+M+KLD+FV DLKIWLSGALTYEQT
Sbjct: 122 LAKDPRTNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQT 181

Query: 183 CLDGFENTTGDAGEKMQKFLKSAQQMTTNGLAIVTELSTIMASM-MPGMTGVGRRLL--- 242
           CLDGFENTTGDAG +MQ+FLKSAQQMTTNGL IV ELSTI+ S+ +PGM+  GRRLL   
Sbjct: 182 CLDGFENTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQLPGMS--GRRLLEDD 241

Query: 243 DEGVPSWVSDGKRRLMQAGPGGLKPDVVVAQDGSGKYKTINAALVDVPKKSNKTFVIYVK 302
           D  +PSWVSDGKRRLMQAG   +KPD+VVAQDGSGKYKTINAAL DVP KSNKTFVI+VK
Sbjct: 242 DREMPSWVSDGKRRLMQAGAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIHVK 301

Query: 303 AGIYKEIVVVPKALTHVTMYGDGPTKTVVTGSLNFIDGIQTFKTATFCKSTFFSTLCILL 362
           AGIYKEIVV+PK +TH+TMYGDGPTKTVVTGSLNFIDGIQTFKTATF             
Sbjct: 302 AGIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATF------------- 361

Query: 363 YMLKPNESEFMFETAVVGSDFFAKDMGFVNSAGAAKHQAVALRVQSDRSIFYNCQMDGYQ 422
                         + +G++F+A+DMGF N+AGAAKHQAVALRVQSDRSIF+NCQ+DGYQ
Sbjct: 362 --------------SAIGANFYARDMGFENTAGAAKHQAVALRVQSDRSIFFNCQIDGYQ 421

Query: 423 DTLYAHAHRQFYRDCSISGTIDFVFGNSAAIFQNCKLIVRKPMDNQQCIVTAQGRMNRRE 482
           DTLYAHAHRQFYRDC+ISGTIDFVFGN+A  FQNCKL+VRKP+DNQQCIVTA GR+NR+E
Sbjct: 422 DTLYAHAHRQFYRDCTISGTIDFVFGNAATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKE 481

Query: 483 PTALVFQNCHFMGDPAYLPFKAINKAYLGRPWKAYSRTLIMGSQIDDLIQPEGWLPWGGD 542
           PTAL+FQ+CHFMGDPAYLPFKAINKAYLGRPWK YSRT+I+GS IDD+IQPEGWLPW GD
Sbjct: 482 PTALIFQSCHFMGDPAYLPFKAINKAYLGRPWKEYSRTIIIGSTIDDIIQPEGWLPWMGD 541

Query: 543 FGLTTLFYAEVQNKGPGADLSKRVKWRGIKTITPDHAAKFTPRVFIDGDAWIKAKGIPYA 598
           FGL TLFYAEVQNKG GAD SKRVKWRGIK ITP HAA FTPR FIDGDAWI AKGIPY+
Sbjct: 542 FGLNTLFYAEVQNKGAGADESKRVKWRGIKHITPQHAADFTPRRFIDGDAWIPAKGIPYS 576

[Close]

BLAST of Spg025082 vs. ExPASy TrEMBL
Match: A0A6J1H4H8 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111460411 PE=3 SV=1)

HSP 1 Score: 899.0 bits (2322), Expect = 1.0e-257
Identity = 445/607 (73.31%), Postives = 501/607 (82.54%), Query Frame = 0


Query: 3   SNGFANAEGDQKKKKMVIISVSSLFLVAMVVAVTVGVNNTPHVGNTDDGG---------- 62
           SNGF +AE D+KKKK+ +ISVSSL LVAMVVAVTVGVNN P     D GG          
Sbjct: 2   SNGF-DAESDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDHG 61

Query: 63  --AAEISTSTKAIQAICQPTDYKETCEQGLMAEAGNTTDPKELVKAGFKVATAYINSAIK 122
             A +IS+STKAI+ ICQPTDYKETCE+ LM EAGNTTDPKELVKAGFK+A   +N+AI+
Sbjct: 62  DDAEKISSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAIR 121

Query: 123 NSTTLQELAKDPRTNQALQNCRELLEYAIDDLTQSFDRIGTFEMTKLDEFVNDLKIWLSG 182
           NSTTLQELA+DPR NQALQNCRELLEYAIDDL  S+ RIGTF ++K +EFVNDLKIWLS 
Sbjct: 122 NSTTLQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLSA 181

Query: 183 ALTYEQTCLDGFENTTGDAGEKMQKFLKSAQQMTTNGLAIVTELSTIMASMMPGMTGVGR 242
           ALTYEQTCLDGFENTTGD+GE+M++FLKSAQ+MTTNGL IV+ELS I+ + +      GR
Sbjct: 182 ALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSKILGT-LDAPAATGR 241

Query: 243 RLLDEGVPSWVSDGKRRLMQAGPGGLKPDVVVAQDGSGKYKTINAALVDVPKKSNKTFVI 302
           RLLD  +P WVSDGKRRLMQ G G +KPD+VVAQDGSGKYKTIN ALV VP KSNKTFVI
Sbjct: 242 RLLDTDIPLWVSDGKRRLMQVGTGAIKPDLVVAQDGSGKYKTINEALVHVPMKSNKTFVI 301

Query: 303 YVKAGIYKEIVVVPKALTHVTMYGDGPTKTVVTGSLNFIDGIQTFKTATFCKSTFFSTLC 362
           YVKAGIYKEIVV+PK +TH+TMYGDGPTK+VVTG+LNF+DGIQTFKTATF          
Sbjct: 302 YVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATF---------- 361

Query: 363 ILLYMLKPNESEFMFETAVVGSDFFAKDMGFVNSAGAAKHQAVALRVQSDRSIFYNCQMD 422
                            A +G++FF +DMGFVN+AG  KHQAVALRVQSDR+I YNCQ+D
Sbjct: 362 -----------------AAIGANFFVRDMGFVNTAGPTKHQAVALRVQSDRAIIYNCQID 421

Query: 423 GYQDTLYAHAHRQFYRDCSISGTIDFVFGNSAAIFQNCKLIVRKPMDNQQCIVTAQGRMN 482
           GYQDTLYAHA+RQFYRDC ISGTIDFVFGNSAAIFQNCKL+VRKP+DNQQCIVTA GR+N
Sbjct: 422 GYQDTLYAHANRQFYRDCIISGTIDFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLN 481

Query: 483 RREPTALVFQNCHFMGDPAYLPFKAINKAYLGRPWKAYSRTLIMGSQIDDLIQPEGWLPW 542
           RREPTAL+FQ CHFMGDP+YLPFK +NKAYLGRPWK YSRT+IMGSQIDDLIQPEGWLPW
Sbjct: 482 RREPTALIFQGCHFMGDPSYLPFKKVNKAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPW 541

Query: 543 GGDFGLTTLFYAEVQNKGPGADLSKRVKWRGIKTITPDHAAKFTPRVFIDGDAWIKAKGI 598
            GDF L TLFYAEVQN+GPGAD S RVKWRGI+ ITP HAA +T R FIDGDAWI  +G+
Sbjct: 542 MGDFALNTLFYAEVQNRGPGADQSHRVKWRGIRHITPQHAAAYTARRFIDGDAWIPVRGV 579

[Close]

BLAST of Spg025082 vs. ExPASy TrEMBL
Match: A0A6J1KZN0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111499042 PE=3 SV=1)

HSP 1 Score: 896.0 bits (2314), Expect = 8.7e-257
Identity = 449/629 (71.38%), Postives = 505/629 (80.29%), Query Frame = 0


Query: 3   SNGFANAEGDQKKKKMVIISVSSLFLVAMVVAVTVGVNNTPHVGNTDDGGAAE------- 62
           SNGF +AEGD+KKKK+ +ISVSSL LVAMVVAVTVGVNN PH    D GG  +       
Sbjct: 2   SNGF-DAEGDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPHEDLDDGGGGGDLHDHGDD 61

Query: 63  --------------------------ISTSTKAIQAICQPTDYKETCEQGLMAEAGNTTD 122
                                     IS+STKAI+ ICQPTDYKETCE+ LM EAGNTTD
Sbjct: 62  GGDVHDHGDYGGGGGDAHDHGDDGEKISSSTKAIKTICQPTDYKETCEESLMVEAGNTTD 121

Query: 123 PKELVKAGFKVATAYINSAIKNSTTLQELAKDPRTNQALQNCRELLEYAIDDLTQSFDRI 182
            KELVKAGFK+A   +N+AI+NSTTLQELAKDPR NQALQNCRELLEYAIDDL  S+ RI
Sbjct: 122 LKELVKAGFKIAVEKLNAAIRNSTTLQELAKDPRANQALQNCRELLEYAIDDLHDSYHRI 181

Query: 183 GTFEMTKLDEFVNDLKIWLSGALTYEQTCLDGFENTTGDAGEKMQKFLKSAQQMTTNGLA 242
           GTF ++K +EFVNDLKIWLS ALTYEQTCLDGFENTTGD+GE+M++FLKSAQ+MTTNGL 
Sbjct: 182 GTFHVSKFEEFVNDLKIWLSAALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLG 241

Query: 243 IVTELSTIMASM-MPGMTGVGRRLLDEGVPSWVSDGKRRLMQAGPGGLKPDVVVAQDGSG 302
           IV+ELS I+ ++  P  T  GRRLLD  +P WVSDGKRRLMQ   G +KPD+VVAQDGSG
Sbjct: 242 IVSELSNILGTLHEPAAT--GRRLLDTDMPLWVSDGKRRLMQLETGAIKPDLVVAQDGSG 301

Query: 303 KYKTINAALVDVPKKSNKTFVIYVKAGIYKEIVVVPKALTHVTMYGDGPTKTVVTGSLNF 362
           KYKTIN ALVDVP KSNKTFVIYVKAGIYKEIVV+PK +TH+TMYGDGPTK+VVTG+LNF
Sbjct: 302 KYKTINEALVDVPMKSNKTFVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNF 361

Query: 363 IDGIQTFKTATFCKSTFFSTLCILLYMLKPNESEFMFETAVVGSDFFAKDMGFVNSAGAA 422
           +DGIQTFKTATF                           A +G++FF +DMGFVNSAG  
Sbjct: 362 VDGIQTFKTATF---------------------------AAIGANFFVRDMGFVNSAGPT 421

Query: 423 KHQAVALRVQSDRSIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTIDFVFGNSAAIFQNC 482
           KHQAVALR+QSDR+I YNCQ+DG+QDTLYAHA+RQFYRDC+ISGTIDFVFGNSAAIFQNC
Sbjct: 422 KHQAVALRIQSDRAIIYNCQIDGFQDTLYAHANRQFYRDCTISGTIDFVFGNSAAIFQNC 481

Query: 483 KLIVRKPMDNQQCIVTAQGRMNRREPTALVFQNCHFMGDPAYLPFKAINKAYLGRPWKAY 542
           KL+VRKP+DNQQCIVTA GR+NRREPTAL+FQ CHFMGDPAYLPFK +NKAYLGRPWK Y
Sbjct: 482 KLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPAYLPFKKVNKAYLGRPWKQY 541

Query: 543 SRTLIMGSQIDDLIQPEGWLPWGGDFGLTTLFYAEVQNKGPGADLSKRVKWRGIKTITPD 598
           SRT+IMGSQIDDLIQPEGWLPW GDFGL TLFYAEVQN+GPGAD S RVKWRGI+ ITP 
Sbjct: 542 SRTIIMGSQIDDLIQPEGWLPWMGDFGLNTLFYAEVQNRGPGADQSHRVKWRGIRHITPQ 600

[Close]

BLAST of Spg025082 vs. ExPASy TrEMBL
Match: A0A6J1ER80 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111436828 PE=3 SV=1)

HSP 1 Score: 882.5 bits (2279), Expect = 1.0e-252
Identity = 438/600 (73.00%), Postives = 496/600 (82.67%), Query Frame = 0


Query: 3   SNGFANAEGDQKKKKMVIISVSSLFLVAMVVAVTVGVNNTPH----VGNTDDGGAAEIST 62
           SNGF +AEGD +KKKM ++ VSSLFLVAMVVAVTVGVNN+ H    +GN    GA EIS+
Sbjct: 2   SNGF-DAEGDNEKKKMALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGNGHGDGAGEISS 61

Query: 63  STKAIQAICQPTDYKETCEQGLMAEAGNTTDPKELVKAGFKVATAYINSAIKNSTTLQEL 122
           STKAIQ IC+PTDYKETCE+ LM+E  NTTDP+ELVK GFKV    +NSAI+NSTTLQ L
Sbjct: 62  STKAIQTICRPTDYKETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQAL 121

Query: 123 AKDPRTNQALQNCRELLEYAIDDLTQSFDRIGTFEMTKLDEFVNDLKIWLSGALTYEQTC 182
           AK+PR NQALQNCRELLEYAIDD+T SF+RIGTF+M KLD+FV DL +WLSGALTYEQTC
Sbjct: 122 AKEPRANQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTC 181

Query: 183 LDGFENTTGDAGEKMQKFLKSAQQMTTNGLAIVTELSTIMASMMPGMTGVGRRLL-DEGV 242
           LDGFENTTGDAGE+M+KFLKSAQ+MT+NGLAIVTEL  ++ S+    +  GRRLL  + V
Sbjct: 182 LDGFENTTGDAGEQMKKFLKSAQEMTSNGLAIVTELKNMLGSLQG--SKAGRRLLAGDEV 241

Query: 243 PSWVSDGKRRLMQAGPGGLKPDVVVAQDGSGKYKTINAALVDVPKKSNKTFVIYVKAGIY 302
           PSWVSD KRRLMQ     + P+VVVAQDGSGKYKTIN ALV+V KKSN TFVIYVKAGIY
Sbjct: 242 PSWVSDEKRRLMQVPTAAITPNVVVAQDGSGKYKTINEALVEVLKKSNITFVIYVKAGIY 301

Query: 303 KEIVVVPKALTHVTMYGDGPTKTVVTGSLNFIDGIQTFKTATFCKSTFFSTLCILLYMLK 362
           KE V++PKA+THVTMYGDGPTKTVV+GSLNF+DGIQTFKTATF                 
Sbjct: 302 KETVLIPKAMTHVTMYGDGPTKTVVSGSLNFVDGIQTFKTATF----------------- 361

Query: 363 PNESEFMFETAVVGSDFFAKDMGFVNSAGAAKHQAVALRVQSDRSIFYNCQMDGYQDTLY 422
                     AVVG++FF +DMGF N+AGA KHQAVALRVQSDRSIF+NC MDG+QDTLY
Sbjct: 362 ----------AVVGANFFCRDMGFENTAGATKHQAVALRVQSDRSIFFNCHMDGHQDTLY 421

Query: 423 AHAHRQFYRDCSISGTIDFVFGNSAAIFQNCKLIVRKPMDNQQCIVTAQGRMNRREPTAL 482
           A  HRQFYRDC +SGTIDFVFGNSAA+FQNC L+VRKP+ NQQCIVTAQGR+  REPT L
Sbjct: 422 AQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCNLVVRKPLANQQCIVTAQGRLENREPTGL 481

Query: 483 VFQNCHFMGDPAYLPFKAINKAYLGRPWKAYSRTLIMGSQIDDLIQPEGWLPWGGDFGLT 542
           VFQNCHFMGDPAYLPFKA+NKAYLGRPWK +SRT+IMGS+IDDLIQPEGWLPW GDFGL 
Sbjct: 482 VFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFSRTIIMGSEIDDLIQPEGWLPWMGDFGLN 541

Query: 543 TLFYAEVQNKGPGADLSKRVKWRGIKTITPDHAAKFTPRVFIDGDAWIKAKGIPYASGMM 598
           TLFYAE QNKGPGADL+ RVKW+GIKT+TP  AA FTP++FIDGD WI   G+PY+ GMM
Sbjct: 542 TLFYAEFQNKGPGADLTNRVKWQGIKTLTPQQAADFTPKIFIDGDDWILNTGVPYSPGMM 571

[Close]

BLAST of Spg025082 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 644.0 bits (1660), Expect = 1.2e-184
Identity = 313/588 (53.23%), Postives = 417/588 (70.92%), Query Frame = 0


Query: 13  QKKKKMVIISVSSLFLVAMVVAVTVGVNNTPHVGNTDDGGAAEISTSTKAIQAICQPTDY 72
           ++K++ ++I++SS+ L++MVVAVTVGV+   H G  D  G AE++ S KA++ +C PTDY
Sbjct: 10  KRKRRYIVITISSVLLISMVVAVTVGVSLNKHDG--DSKGKAEVNASVKAVKDVCAPTDY 69

Query: 73  KETCEQGLMAEAGNTTDPKELVKAGFKVATAYINSAIKNSTTLQELAKDPRTNQALQNCR 132
           ++TCE  L+    NTTDP ELVK  F V    I  A K S T+ EL KD RT  AL  C+
Sbjct: 70  RKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSRTRMALDQCK 129

Query: 133 ELLEYAIDDLTQSFDRIGTFEMTKLDEFVNDLKIWLSGALTYEQTCLDGFENTTGDAGEK 192
           EL++YA+D+L+ SF+ +G FE   LDE + +L+IWLS A+++E+TCL+GF+ T G+AGE 
Sbjct: 130 ELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGET 189

Query: 193 MQKFLKSAQQMTTNGLAIVTELSTIMASM-MPGMTGVGRRLLDEGVPSWVSDGKRRLMQA 252
           M+K LK+A ++T NGLAI++E+S  +  M +PG+    RRLL EG PSWV    R+L+QA
Sbjct: 190 MKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLN--SRRLLAEGFPSWVDQRGRKLLQA 249

Query: 253 GP--GGLKPDVVVAQDGSGKYKTINAALVDVPKKSNKTFVIYVKAGIYKEIVVVPKALTH 312
                 +KPD+VVAQDGSG+YKTIN AL  VPKK N TFV+++KAG+YKE V V K ++H
Sbjct: 250 AAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTMSH 309

Query: 313 VTMYGDGPTKTVVTGSLNFIDGIQTFKTATFCKSTFFSTLCILLYMLKPNESEFMFETAV 372
           +   GDGP KT+++G+ N+ DGI T++TAT                            A+
Sbjct: 310 LVFIGDGPDKTIISGNKNYKDGITTYRTAT---------------------------VAI 369

Query: 373 VGSDFFAKDMGFVNSAGAAKHQAVALRVQSDRSIFYNCQMDGYQDTLYAHAHRQFYRDCS 432
           VG+ F AK++GF N+AGA KHQAVA+RVQSD SIF+NC+ DGYQDTLY H+HRQF+RDC+
Sbjct: 370 VGNYFIAKNIGFENTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCT 429

Query: 433 ISGTIDFVFGNSAAIFQNCKLIVRKPMDNQQCIVTAQGRMNRREPTALVFQNCHFMGDPA 492
           ISGTIDF+FG++AA+FQNC L+VRKP+ NQ C +TA GR + RE T  VFQ C   G+P 
Sbjct: 430 ISGTIDFLFGDAAAVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPD 489

Query: 493 YLPFKAINKAYLGRPWKAYSRTLIMGSQIDDLIQPEGWLPWGGDFGLTTLFYAEVQNKGP 552
           YL  K  +KAYLGRPWK YSRT+IM + I D +QP+GW PW GDFGL TLFY+EVQN GP
Sbjct: 490 YLAVKETSKAYLGRPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGP 549

Query: 553 GADLSKRVKWRGIKTITPDHAAKFTPRVFIDGDAWIKAKGIPYASGMM 598
           G+ L+ RV W GIKT++ +   KFTP  +I GD WI  KG+PY +G++
Sbjct: 550 GSALANRVTWAGIKTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGLL 566

[Close]

BLAST of Spg025082 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 634.8 bits (1636), Expect = 7.0e-182
Identity = 316/588 (53.74%), Postives = 410/588 (69.73%), Query Frame = 0


Query: 10  EGDQKKKKMVIISVSSLFLVAMVVAVTVGVNNTPHVGNTDDGGAAEISTSTKAIQAICQP 69
           E  ++KK+ VIIS+SS+ L++MVVAVT+GV+    V  +D+ G  EI+TS KAI+ +C P
Sbjct: 8   EDAKRKKRYVIISISSVLLISMVVAVTIGVS----VNKSDNAGDEEITTSVKAIKDVCAP 67

Query: 70  TDYKETCEQGLMAEAGNTTDPKELVKAGFKVATAYINSAIKNSTTLQELAKDPRTNQALQ 129
           TDYKETCE  L  +A +T+DP ELVK  F      I+   K S T+ EL KDPR   AL 
Sbjct: 68  TDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPRAKMALD 127

Query: 130 NCRELLEYAIDDLTQSFDRIGTFEMTKLDEFVNDLKIWLSGALTYEQTCLDGFENTTGDA 189
            C+EL++YAI +L++SF+ +G FE  K+DE +  L+IWLS  +++EQTCLDGF+ T G+A
Sbjct: 128 QCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNA 187

Query: 190 GEKMQKFLKSAQQMTTNGLAIVTELSTIMASM-MPGMTGVGRRLLDEGVPSWVSDGKRRL 249
           GE ++K LK+A Q+T NGLA+VTE+S  +  M +P M    RRLL +  PSW+    RRL
Sbjct: 188 GETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMN--SRRLLSQEFPSWMDARARRL 247

Query: 250 MQAGPGGLKPDVVVAQDGSGKYKTINAALVDVPKKSNKTFVIYVKAGIYKEIVVVPKALT 309
           + A    +KPD+VVAQDGSG+YKTIN AL  VPKK N TFV+++K GIYKE V V +++T
Sbjct: 248 LNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSMT 307

Query: 310 HVTMYGDGPTKTVVTGSLNFIDGIQTFKTATFCKSTFFSTLCILLYMLKPNESEFMFETA 369
           H+   GDGP KTV++GS ++ DGI T+KTAT                            A
Sbjct: 308 HLVFIGDGPDKTVISGSKSYKDGITTYKTAT---------------------------VA 367

Query: 370 VVGSDFFAKDMGFVNSAGAAKHQAVALRVQSDRSIFYNCQMDGYQDTLYAHAHRQFYRDC 429
           +VG  F AK++ F N+AGA KHQAVA+RV +D SIFYNC+ DGYQDTLYAH+HRQFYRDC
Sbjct: 368 IVGDHFIAKNIAFENTAGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDC 427

Query: 430 SISGTIDFVFGNSAAIFQNCKLIVRKPMDNQQCIVTAQGRMNRREPTALVFQNCHFMGDP 489
           +ISGTIDF+FG++AA+FQNC L+VRKP+ NQ C +TA GR + RE T  V Q C  +G+P
Sbjct: 428 TISGTIDFLFGDAAAVFQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEP 487

Query: 490 AYLPFKAINKAYLGRPWKAYSRTLIMGSQIDDLIQPEGWLPWGGDFGLTTLFYAEVQNKG 549
            YL  K  +K YLGRPWK YSRT+IM + I D + PEGW PW G+FGL TLFY+EVQN G
Sbjct: 488 DYLAVKEQSKTYLGRPWKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTG 547

Query: 550 PGADLSKRVKWRGIKTITPDHAAKFTPRVFIDGDAWIKAKGIPYASGM 597
           PGA ++KRV W GIK ++ +   KFTP  +I GDAWI  KG+PY  G+
Sbjct: 548 PGAAITKRVTWPGIKKLSDEEILKFTPAQYIQGDAWIPGKGVPYILGL 562

[Close]

BLAST of Spg025082 vs. TAIR 10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 583.2 bits (1502), Expect = 2.4e-166
Identity = 286/593 (48.23%), Postives = 398/593 (67.12%), Query Frame = 0


Query: 10  EGDQKKKKMVIISVSSLFLVAMVVAVTVGVNNTPHVGNTDDGGAAEISTSTKAIQAICQP 69
           +G+ KKKK +I  V +  LV MVVA  VG+  + +  +++     +I T+T A++A+C P
Sbjct: 4   DGELKKKKCIIAGVITALLVLMVVA--VGITTSRNTSHSEKIVPVQIKTATTAVEAVCAP 63

Query: 70  TDYKETCEQGLMAEAGNTTDPKELVKAGFKVATAYINSAIKNSTT--LQELAKDPRTNQA 129
           TDYKETC   LM  + ++T P +L+K GF V    I  +IK ++     + A D  T  A
Sbjct: 64  TDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGA 123

Query: 130 LQNCRELLEYAIDDLTQSFDRIGTFEMTKLDEFVNDLKIWLSGALTYEQTCLDGFENTTG 189
           L+ C +L+  A DDL +  D    F + ++++FV DL++WLSG++ Y+QTC+D FE T  
Sbjct: 124 LELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNS 183

Query: 190 DAGEKMQKFLKSAQQMTTNGLAIVTELSTIMASM-MPGMTG----VGRRLL--DEGVPSW 249
              + MQK  K+++++T+NGLA++T +S ++    + G+TG      R+LL  ++G+PSW
Sbjct: 184 KLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSAEDGIPSW 243

Query: 250 VSDGKRRLMQAGPGGLKPDVVVAQDGSGKYKTINAALVDVPKKSNKTFVIYVKAGIYKEI 309
           V    RRLM A  GG+K +VVVA DGSG+YKTIN AL  VPK + K FVIY+K G+Y E 
Sbjct: 244 VGPNTRRLM-ATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVYNEK 303

Query: 310 VVVPKALTHVTMYGDGPTKTVVTGSLNFIDG-IQTFKTATFCKSTFFSTLCILLYMLKPN 369
           V V K +THVT  GDGPTKT +TGSLN+  G ++T+ TAT                    
Sbjct: 304 VDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTAT-------------------- 363

Query: 370 ESEFMFETAVVGSDFFAKDMGFVNSAGAAKHQAVALRVQSDRSIFYNCQMDGYQDTLYAH 429
                   A+ G +F AK++GF N+AG   HQAVALRV +D ++FYNCQ+DGYQDTLY H
Sbjct: 364 -------VAINGDNFTAKNIGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVH 423

Query: 430 AHRQFYRDCSISGTIDFVFGNSAAIFQNCKLIVRKPMDNQQCIVTAQGRMNRREPTALVF 489
           +HRQF+RDC++SGT+DF+FG+   + QNC ++VRKPM +Q C++TAQGR ++RE T LV 
Sbjct: 424 SHRQFFRDCTVSGTVDFIFGDGIVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVL 483

Query: 490 QNCHFMGDPAYLPFKAINKAYLGRPWKAYSRTLIMGSQIDDLIQPEGWLPWGGDFGLTTL 549
           QNCH  G+PAY+P K+INKAYLGRPWK +SRT+IMG+ IDD+I P GWLPW GDF L TL
Sbjct: 484 QNCHITGEPAYIPVKSINKAYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTL 543

Query: 550 FYAEVQNKGPGADLSKRVKWRGIKTITPDHAAKFTPRVFIDGDAWIKAKGIPY 593
           +YAE +N GPG++ ++RVKW GIK ++P  A +FTP  F+ G+ WI    +PY
Sbjct: 544 YYAEYENNGPGSNQAQRVKWPGIKKLSPKQALRFTPARFLRGNLWIPPNRVPY 566

[Close]

BLAST of Spg025082 vs. TAIR 10
Match: AT3G06830.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 527.3 bits (1357), Expect = 1.6e-149
Identity = 275/599 (45.91%), Postives = 386/599 (64.44%), Query Frame = 0


Query: 9   AEGDQKKKKMVIISVSSLFLVAMVVAVTVGVNNTPHVGNTDDGGAAEISTSTKAIQAICQ 68
           ++GD+KKK +V  SVS  FLV MVV+V V  +   H    D+     I  +TKA+QA+C 
Sbjct: 3   SDGDKKKKFIVAGSVSG-FLVIMVVSVAVVTSK--HSPKDDEN---HIRKTTKAVQAVCA 62

Query: 69  PTDYKETCEQGLMAEAGNTTDPKELVKAGFKVATAYINSAIKNST--TLQELAKDPRTNQ 128
           PTD+K+TC   LM  + ++ DP +L+K GFKV    IN +++ ++     +  K+P    
Sbjct: 63  PTDFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKG 122

Query: 129 ALQNCRELLEYAIDDLTQSFDRIGTFEMTKLDEFVNDLKIWLSGALTYEQTCLDGFENTT 188
           A + C +L+  AIDDL +  D    F + +++ FV DL++WLSG++ ++QTC+D F    
Sbjct: 123 AFELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIK 182

Query: 189 GDAGEKMQKFLKSAQQMTTNGLAIVTELSTIMA-SMMPGMTGV----GRRLL--DEGVPS 248
            +  + M K  K+++++++N LA+VT +ST++  S + G+TG      R+LL  ++ +P+
Sbjct: 183 SNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLSTEDSIPT 242

Query: 249 WVSDGKRRLMQA---GPGGLKPDVVVAQDGSGKYKTINAALVDVPKKSNKTFVIYVKAGI 308
           WV    RRLM A   GPG +K + VVAQDG+G++KTI  AL  VPK +   F+I++K GI
Sbjct: 243 WVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGNKVPFIIHIKEGI 302

Query: 309 YKEIVVVPKALTHVTMYGDGPTKTVVTGSLNF-IDGIQTFKTATFCKSTFFSTLCILLYM 368
           YKE V V K + HVT  GDGP KT++TGSLNF I  ++TF TAT                
Sbjct: 303 YKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATI--------------- 362

Query: 369 LKPNESEFMFETAVVGSDFFAKDMGFVNSAGAAKHQAVALRVQSDRSIFYNCQMDGYQDT 428
                        + G  F AK++G  N+AG    QAVALRV +D ++F++CQ+DG+QDT
Sbjct: 363 ------------TIEGDHFTAKNIGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDT 422

Query: 429 LYAHAHRQFYRDCSISGTIDFVFGNSAAIFQNCKLIVRKPMDNQQCIVTAQGRMNRREPT 488
           LY H+HRQFYRDC++SGT+DF+FG++  I QNCK++VRKP   Q C+VTAQGR N RE T
Sbjct: 423 LYVHSHRQFYRDCTVSGTVDFIFGDAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVREST 482

Query: 489 ALVFQNCHFMGDPAYLPFKAINKAYLGRPWKAYSRTLIMGSQIDDLIQPEGWLPWGGDFG 548
            LV   CH  GDPAY+P K++NKAYLGRPWK +SRT+IM + IDD+I P GWLPW GDF 
Sbjct: 483 GLVLHGCHITGDPAYIPMKSVNKAYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFA 542

Query: 549 LTTLFYAEVQNKGPGADLSKRVKWRGIKTITPDHAAKFTPRVFIDGDAWIKAKGIPYAS 595
           L TL+YAE  N GPG++ ++RVKW GIK +TP  A  +T   F+ GD WI    +PY +
Sbjct: 543 LKTLYYAEHMNTGPGSNQAQRVKWPGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

[Close]

BLAST of Spg025082 vs. TAIR 10
Match: AT4G15980.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 453.4 bits (1165), Expect = 2.9e-127
Identity = 269/726 (37.05%), Postives = 372/726 (51.24%), Query Frame = 0


Query: 17  KMVIISVSSLFLVAMVVAVTVGVNNTPHVGNTDDGGAAEISTSTKAIQAICQPTDYKETC 76
           K V++ V++L ++AMV+ V     N+P     +    +++  +   +  IC  TDYK+ C
Sbjct: 3   KYVLLGVTAL-IMAMVICVEANDGNSPSRKMEEVHRESKLMITKTTVSIICASTDYKQDC 62

Query: 77  EQGLMAEAGNTTDPKELVKAGFKVATAYINSAI-KNSTTLQELA-KDPRTNQALQNCREL 136
              L      + DP+ L+++ F +A   I S I +    L+  A  D  T +AL  CREL
Sbjct: 63  TTSLATV--RSPDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADMHTREALNTCREL 122

Query: 137 LEYAIDDLTQSFDRIGTFEMTKLDEFVNDLKIWLSGALTYEQTCLDGFENTTGDAGEKMQ 196
           ++ AIDDL ++ D+   F  T+L +FV DL +WLSG++TY+QTC+DGFE    +A   M+
Sbjct: 123 MDDAIDDLRKTRDKFRGFLFTRLSDFVEDLCVWLSGSITYQQTCIDGFEGIDSEAAVMME 182

Query: 197 KFLKSAQQMTTNGLAIVTELSTIM-ASMMP-----------GMTGVG------------- 256
           + ++  Q +T+NGLAI   L  ++ A  +P           G+ G G             
Sbjct: 183 RVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILGSGSSRDESVGSSQDS 242

Query: 257 ------------------------------------------------------------ 316
                                                                       
Sbjct: 243 PPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQQMESSEDTPKKSAFS 302

Query: 317 -----------------------------------------------------------R 376
                                                                      R
Sbjct: 303 GNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPLRKLNPLNKLDSLKDR 362

Query: 377 RLLDEG-VPSWVSDGKRRLMQAGP--GGLKPDVVVAQDGSGKYKTINAALVDVPKKSNKT 436
            L +EG  P WV+   RRL+   P   G+K +VVVA+DGSGK KTI  AL  VP K+ K 
Sbjct: 363 HLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKTIAQALAMVPMKNTKK 422

Query: 437 FVIYVKAGIYKEIVVVPKALTHVTMYGDGPTKTVVTGSLNFI-DGIQTFKTATFCKSTFF 496
           FVI++K G+YKE V V K + HV   GDGPTKTV+TG + F+ D + T++TA+       
Sbjct: 423 FVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQVGTYRTAS------- 482

Query: 497 STLCILLYMLKPNESEFMFETAVVGSDFFAKDMGFVNSAGAAKHQAVALRVQSDRSIFYN 556
                                AV G  F AKD+GF N+AGAA+HQAVALRV +D ++F+N
Sbjct: 483 --------------------VAVNGDYFMAKDIGFENTAGAARHQAVALRVSADFAVFFN 542

Query: 557 CQMDGYQDTLYAHAHRQFYRDCSISGTIDFVFGNSAAIFQNCKLIVRKPMDNQQCIVTAQ 593
           C M+GYQDTLY H HRQFYR+C +SGTIDFVFG++ A+FQNC+ ++R+PM++QQCIVTAQ
Sbjct: 543 CHMNGYQDTLYVHTHRQFYRNCRVSGTIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQ 602

The following BLAST results are available for this feature:

BLAST of Spg025082 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008458409.1	2.3e-272	78.61	PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 [Cucumis melo]	[more]
XP_004150503.1	8.4e-270	78.31	probable pectinesterase/pectinesterase inhibitor 21 [Cucumis sativus] >KGN47423....	[more]
XP_038875843.1	2.4e-269	77.87	probable pectinesterase/pectinesterase inhibitor 21 [Benincasa hispida]	[more]
XP_023549205.1	1.5e-258	73.68	probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita pepo subsp. pepo]	[more]
XP_022959432.1	2.1e-257	73.31	probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita moschata]	[more]

BLAST of Spg025082 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GX86	1.6e-183	53.23	Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3...	[more]
Q3E8Z8	9.8e-181	53.74	Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3...	[more]
Q9FJ21	3.4e-165	48.23	Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3...	[more]
Q8GXA1	2.2e-148	45.91	Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3...	[more]
Q42920	2.3e-129	49.69	Pectinesterase/pectinesterase inhibitor OS=Medicago sativa OX=3879 PE=2 SV=1	[more]

BLAST of Spg025082 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3C7B6	1.1e-272	78.61	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497833 PE=3 SV=1	[more]
A0A0A0KD24	4.0e-270	78.31	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G318700 PE=3 SV=1	[more]
A0A6J1H4H8	1.0e-257	73.31	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111460411 PE=3 SV=1	[more]
A0A6J1KZN0	8.7e-257	71.38	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111499042 PE=3 SV=1	[more]
A0A6J1ER80	1.0e-252	73.00	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111436828 PE=3 SV=1	[more]

BLAST of Spg025082 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G05610.1	1.2e-184	53.23	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G27870.1	7.0e-182	53.74	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G49180.1	2.4e-166	48.23	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G06830.1	1.6e-149	45.91	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G15980.1	2.9e-127	37.05	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 58..210 e-value: 9.2E-37 score: 138.1
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 62..210 e-value: 3.8E-27 score: 95.5
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 58..214 e-value: 1.3E-24 score: 84.5
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 259..342 e-value: 2.2E-28 score: 98.8 coord: 368..582 e-value: 3.6E-102 score: 341.1
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 57..215 e-value: 5.0E-34 score: 119.5
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 61..214
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 254..596 e-value: 1.5E-113 score: 381.4
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 20..340 coord: 367..595
None	No IPR available	PANTHER	PTHR31707:SF131	PECTINESTERASE	coord: 20..340 coord: 367..595
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 65..216 e-value: 1.31243E-45 score: 156.444
IPR018040	Pectinesterase, Tyr active site	PROSITE	PS00800	PECTINESTERASE_1	coord: 284..303
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 430..439
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 257..594

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg025082.1	Spg025082.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity