Spg025018 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg025018 vs. NCBI nr
Match: XP_038876934.1 (mitochondrial-processing peptidase subunit alpha-like [Benincasa hispida])

HSP 1 Score: 954.1 bits (2465), Expect = 4.7e-274
Identity = 492/505 (97.43%), Postives = 500/505 (99.01%), Query Frame = 0

Query: 1   MYRIAGSRLRSLKNHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLQTPLSG 60
           M+RIAGSRLRSLKNH ANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPL+TPLSG
Sbjct: 1   MHRIAGSRLRSLKNHTANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLETPLSG 60

Query: 61  ITLPPPLPDHVEPSKTKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETPVSSGASHL 120
           ITLPPPLPDHVEPSKTKITTLPNGVRIASETSSNPAASIGIYL+ GSIYETPVSSGASHL
Sbjct: 61  ITLPPPLPDHVEPSKTKITTLPNGVRIASETSSNPAASIGIYLNSGSIYETPVSSGASHL 120

Query: 121 LERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVDCVR 180
           LERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVDCVR
Sbjct: 121 LERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVDCVR 180

Query: 181 NPVFLDWEINEELQKLKAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDG 240
           NPVFLDWEINEELQKLKAE+GEL+NNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDG
Sbjct: 181 NPVFLDWEINEELQKLKAEIGELANNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDG 240

Query: 241 TILEEFIAENYTAPRMVLAASGVEHEELLSIAEPLLSDLPKVPSPKVPESAYVGGDYRRQ 300
           TILEEFIAENYTAPRMVLAASGVEHEELLSIAEPLLSDLPKVP+ KVPESAYVGGDYRRQ
Sbjct: 241 TILEEFIAENYTAPRMVLAASGVEHEELLSIAEPLLSDLPKVPNVKVPESAYVGGDYRRQ 300

Query: 301 ADCGSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNE 360
           ADCGSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNE
Sbjct: 301 ADCGSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNE 360

Query: 361 YQQIQSFSAFNSIFNNTGLFGIYASTGSDFVAQAVDIAASELISIATPGQVSEVQLNRAK 420
           YQQIQSFSAFNSIFNNTGLFGIYASTGS+FVAQAVDIA SELISIATPGQVSEVQLNRAK
Sbjct: 361 YQQIQSFSAFNSIFNNTGLFGIYASTGSNFVAQAVDIATSELISIATPGQVSEVQLNRAK 420

Query: 421 ESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVISSP 480
           ESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVISSP
Sbjct: 421 ESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVISSP 480

Query: 481 LTMGSYGDVINVPTYESVNSKFHRK 506
           LTM SYGDVINVPTYESVNS+FHRK
Sbjct: 481 LTMASYGDVINVPTYESVNSRFHRK 505

BLAST of Spg025018 vs. NCBI nr
Match: XP_023527682.1 (mitochondrial-processing peptidase subunit alpha-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 951.0 bits (2457), Expect = 4.0e-273
Identity = 489/503 (97.22%), Postives = 497/503 (98.81%), Query Frame = 0

Query: 1   MYRIAGSRLRSLKNHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLQTPLSG 60
           M+RIAGSRLRSLKNHAANLGASRFATS AVAARPTSGGFFSWLTGERSSSLPPL+TPLSG
Sbjct: 1   MHRIAGSRLRSLKNHAANLGASRFATSGAVAARPTSGGFFSWLTGERSSSLPPLETPLSG 60

Query: 61  ITLPPPLPDHVEPSKTKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETPVSSGASHL 120
           ITLPPPLPD+VEPSKTKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETPVSSGASHL
Sbjct: 61  ITLPPPLPDYVEPSKTKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETPVSSGASHL 120

Query: 121 LERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVDCVR 180
           LERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTY PEMVELLVDCVR
Sbjct: 121 LERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTYSPEMVELLVDCVR 180

Query: 181 NPVFLDWEINEELQKLKAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDG 240
           NPVFLDWEINEELQKLKAE+ ELSNNPQGLLLEAIHSAGYSGALANPLVAPE+ALSRLDG
Sbjct: 181 NPVFLDWEINEELQKLKAEIAELSNNPQGLLLEAIHSAGYSGALANPLVAPEYALSRLDG 240

Query: 241 TILEEFIAENYTAPRMVLAASGVEHEELLSIAEPLLSDLPKVPSPKVPESAYVGGDYRRQ 300
           TILEEFIA+NYTAPRMVLAASGVEHEELLSIAEPLLSDLPKVPSPKVPESAYVGGDYRRQ
Sbjct: 241 TILEEFIADNYTAPRMVLAASGVEHEELLSIAEPLLSDLPKVPSPKVPESAYVGGDYRRQ 300

Query: 301 ADCGSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNE 360
           ADCGSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNE
Sbjct: 301 ADCGSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNE 360

Query: 361 YQQIQSFSAFNSIFNNTGLFGIYASTGSDFVAQAVDIAASELISIATPGQVSEVQLNRAK 420
           YQQIQSFSAFNSIFNNTGLFGIYASTGSDFVA AVDIAASELISIATPGQVSEVQLNRAK
Sbjct: 361 YQQIQSFSAFNSIFNNTGLFGIYASTGSDFVAHAVDIAASELISIATPGQVSEVQLNRAK 420

Query: 421 ESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVISSP 480
           ESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKV+SSP
Sbjct: 421 ESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVMSSP 480

Query: 481 LTMGSYGDVINVPTYESVNSKFH 504
           LTM SYGDVINVPTYESVN +FH
Sbjct: 481 LTMASYGDVINVPTYESVNRRFH 503

BLAST of Spg025018 vs. NCBI nr
Match: XP_022948871.1 (mitochondrial-processing peptidase subunit alpha-like [Cucurbita moschata])

HSP 1 Score: 948.3 bits (2450), Expect = 2.6e-272
Identity = 490/505 (97.03%), Postives = 497/505 (98.42%), Query Frame = 0

Query: 1   MYRIAGSRLRSLKNHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLQTPLSG 60
           M+RIAGSRLR LKNHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPL+TPLS 
Sbjct: 1   MHRIAGSRLRLLKNHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLETPLSD 60

Query: 61  ITLPPPLPDHVEPSKTKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETPVSSGASHL 120
           ITLPPPLPDHVEPSKTKITTLPNG RIASETSSNPAASIGIYLDCGSIYETP SSGASHL
Sbjct: 61  ITLPPPLPDHVEPSKTKITTLPNGARIASETSSNPAASIGIYLDCGSIYETPSSSGASHL 120

Query: 121 LERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVDCVR 180
           LERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVDCVR
Sbjct: 121 LERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVDCVR 180

Query: 181 NPVFLDWEINEELQKLKAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDG 240
           NPVFLDWEI EELQKLKAE+GELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDG
Sbjct: 181 NPVFLDWEIKEELQKLKAEIGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDG 240

Query: 241 TILEEFIAENYTAPRMVLAASGVEHEELLSIAEPLLSDLPKVPSPKVPESAYVGGDYRRQ 300
           TILEEFIAENYTAPRMVLAASGVEHEELLSIAEPLLSDLPKVPS KVPESAYVGGDYRRQ
Sbjct: 241 TILEEFIAENYTAPRMVLAASGVEHEELLSIAEPLLSDLPKVPSLKVPESAYVGGDYRRQ 300

Query: 301 ADCGSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNE 360
           ADCGSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNE
Sbjct: 301 ADCGSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNE 360

Query: 361 YQQIQSFSAFNSIFNNTGLFGIYASTGSDFVAQAVDIAASELISIATPGQVSEVQLNRAK 420
           YQQIQSFSAFNSIFN+TGLFGIYASTGS+FVAQAVDIAASELISIATPGQVS+VQLNRAK
Sbjct: 361 YQQIQSFSAFNSIFNDTGLFGIYASTGSNFVAQAVDIAASELISIATPGQVSQVQLNRAK 420

Query: 421 ESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVISSP 480
           ESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVISSP
Sbjct: 421 ESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVISSP 480

Query: 481 LTMGSYGDVINVPTYESVNSKFHRK 506
           LTM SYGDVINVPTYESVNS+FH K
Sbjct: 481 LTMASYGDVINVPTYESVNSRFHSK 505

BLAST of Spg025018 vs. NCBI nr
Match: KAA0050857.1 (mitochondrial-processing peptidase subunit alpha-like [Cucumis melo var. makuwa] >TYK08495.1 mitochondrial-processing peptidase subunit alpha-like [Cucumis melo var. makuwa])

HSP 1 Score: 947.2 bits (2447), Expect = 5.8e-272
Identity = 486/505 (96.24%), Postives = 497/505 (98.42%), Query Frame = 0

Query: 1   MYRIAGSRLRSLKNHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLQTPLSG 60
           M+RIAGSRLRSLKNHAANLGASRFATSSAVA RPTSGGFFSWLTGERSSSLPP++ PLSG
Sbjct: 1   MHRIAGSRLRSLKNHAANLGASRFATSSAVATRPTSGGFFSWLTGERSSSLPPMEIPLSG 60

Query: 61  ITLPPPLPDHVEPSKTKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETPVSSGASHL 120
           I LPPPLPDHVEPSKTKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETP+SSGASHL
Sbjct: 61  INLPPPLPDHVEPSKTKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETPLSSGASHL 120

Query: 121 LERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVDCVR 180
           LERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVDCVR
Sbjct: 121 LERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVDCVR 180

Query: 181 NPVFLDWEINEELQKLKAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDG 240
           NPVFLDWEINEELQKLKAE+GEL+NNPQGLLLEA+HSAGYSGALANPLVAPEFALSRLDG
Sbjct: 181 NPVFLDWEINEELQKLKAEIGELANNPQGLLLEAVHSAGYSGALANPLVAPEFALSRLDG 240

Query: 241 TILEEFIAENYTAPRMVLAASGVEHEELLSIAEPLLSDLPKVPSPKVPESAYVGGDYRRQ 300
           TILEEFIAENYTAPRMVLAASGV+HEELLSIAEPLLSDLPKVP  KVPESAYVGGDYRRQ
Sbjct: 241 TILEEFIAENYTAPRMVLAASGVDHEELLSIAEPLLSDLPKVPGVKVPESAYVGGDYRRQ 300

Query: 301 ADCGSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNE 360
           ADCGSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNE
Sbjct: 301 ADCGSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNE 360

Query: 361 YQQIQSFSAFNSIFNNTGLFGIYASTGSDFVAQAVDIAASELISIATPGQVSEVQLNRAK 420
           YQQIQSFSAFNSIFNNTGLFGIYA TGS+FVA+AVDIAASELISIATPGQVS+VQLNRAK
Sbjct: 361 YQQIQSFSAFNSIFNNTGLFGIYACTGSNFVAKAVDIAASELISIATPGQVSQVQLNRAK 420

Query: 421 ESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVISSP 480
           ESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVISSP
Sbjct: 421 ESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVISSP 480

Query: 481 LTMGSYGDVINVPTYESVNSKFHRK 506
           LTM SYGDVINVPTYESVNS FHRK
Sbjct: 481 LTMASYGDVINVPTYESVNSLFHRK 505

BLAST of Spg025018 vs. NCBI nr
Match: XP_022980477.1 (mitochondrial-processing peptidase subunit alpha-like [Cucurbita maxima])

HSP 1 Score: 945.3 bits (2442), Expect = 2.2e-271
Identity = 487/503 (96.82%), Postives = 496/503 (98.61%), Query Frame = 0

Query: 1   MYRIAGSRLRSLKNHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLQTPLSG 60
           M+RIAGSRLRSLKNHAANLGASRFATS AVAARPTSGGFFSWLTGERSSSLPPL+TPLSG
Sbjct: 1   MHRIAGSRLRSLKNHAANLGASRFATSGAVAARPTSGGFFSWLTGERSSSLPPLETPLSG 60

Query: 61  ITLPPPLPDHVEPSKTKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETPVSSGASHL 120
           ITLPPPLPD+VEPSKTKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETPVSSGASHL
Sbjct: 61  ITLPPPLPDYVEPSKTKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETPVSSGASHL 120

Query: 121 LERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVDCVR 180
           LERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTY PEMVELLVDCVR
Sbjct: 121 LERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTYSPEMVELLVDCVR 180

Query: 181 NPVFLDWEINEELQKLKAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDG 240
           NPVFLDWEI+EELQKLKAE+ ELSNNPQGLLLEAIHSAGYSGALANPLVAPE+ALSRLDG
Sbjct: 181 NPVFLDWEIDEELQKLKAEIAELSNNPQGLLLEAIHSAGYSGALANPLVAPEYALSRLDG 240

Query: 241 TILEEFIAENYTAPRMVLAASGVEHEELLSIAEPLLSDLPKVPSPKVPESAYVGGDYRRQ 300
           TILEEFIA+NYTAPRMVLAASGVEHEELLSIAEPLLSDLPKVPSPKVPESAYVGGDYRRQ
Sbjct: 241 TILEEFIADNYTAPRMVLAASGVEHEELLSIAEPLLSDLPKVPSPKVPESAYVGGDYRRQ 300

Query: 301 ADCGSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNE 360
           AD GSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNE
Sbjct: 301 ADSGSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNE 360

Query: 361 YQQIQSFSAFNSIFNNTGLFGIYASTGSDFVAQAVDIAASELISIATPGQVSEVQLNRAK 420
           YQQIQSFSAFNSIFNNTGLFGIYASTGSDFVA AVDIAASELISIATPGQVSEVQLNRAK
Sbjct: 361 YQQIQSFSAFNSIFNNTGLFGIYASTGSDFVAHAVDIAASELISIATPGQVSEVQLNRAK 420

Query: 421 ESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVISSP 480
           ESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKV+SSP
Sbjct: 421 ESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVMSSP 480

Query: 481 LTMGSYGDVINVPTYESVNSKFH 504
           LTM SYGDVINVPTYESVN +FH
Sbjct: 481 LTMASYGDVINVPTYESVNRRFH 503

BLAST of Spg025018 vs. ExPASy Swiss-Prot
Match: P29677 (Mitochondrial-processing peptidase subunit alpha OS=Solanum tuberosum OX=4113 GN=MPP PE=1 SV=1)

HSP 1 Score: 706.8 bits (1823), Expect = 1.7e-202
Identity = 349/505 (69.11%), Postives = 424/505 (83.96%), Query Frame = 0

Query: 1   MYRIAGSRLRSLKNHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLQTPLSG 60
           MYR A SRL SLK    N   +RF++S+AVA +P SGG FSW+TG+ SSS+ PL  PL+ 
Sbjct: 1   MYRCASSRLSSLKARQGNRVLTRFSSSAAVATKP-SGGLFSWITGDTSSSVTPLDFPLND 60

Query: 61  ITLPPPLPDHVEPSKTKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETPVSSGASHL 120
           + L PPLPD+VEP+KT+ITTL NG+++ASE S NPAASIG+Y+DCGSIYETP S GA+HL
Sbjct: 61  VKLSPPLPDYVEPAKTQITTLANGLKVASEASVNPAASIGLYVDCGSIYETPASYGATHL 120

Query: 121 LERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVDCVR 180
           LER+AFKST NRSHLRIVRE+EAIGGN  ASASRE M YT+DALKTYVP+MVE+L DCVR
Sbjct: 121 LERMAFKSTLNRSHLRIVREIEAIGGNVTASASREHMIYTYDALKTYVPQMVEMLADCVR 180

Query: 181 NPVFLDWEINEELQKLKAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDG 240
           NP FLDWE+ E+L+K+KAE+ E S NPQ LLLEA+HSAGY+G   N L+A E  ++RL+ 
Sbjct: 181 NPAFLDWEVKEQLEKVKAEISEYSKNPQHLLLEAVHSAGYAGPYGNSLMATEATINRLNS 240

Query: 241 TILEEFIAENYTAPRMVLAASGVEHEELLSIAEPLLSDLPKVPSPKVPESAYVGGDYRRQ 300
           T+LEEF+AENYTAPRMVLAASGVEHEE L +AEPLLSDLPKV + + P+  YVGGDYR Q
Sbjct: 241 TVLEEFVAENYTAPRMVLAASGVEHEEFLKVAEPLLSDLPKVATIEEPKPVYVGGDYRCQ 300

Query: 301 ADCGSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNE 360
           AD   TH ALAFEVPGGW  EK+++ LTVLQMLMGGGGSFSAGGPGKGM+SRLYLRVLN+
Sbjct: 301 ADAEMTHFALAFEVPGGWMSEKESMTLTVLQMLMGGGGSFSAGGPGKGMYSRLYLRVLNQ 360

Query: 361 YQQIQSFSAFNSIFNNTGLFGIYASTGSDFVAQAVDIAASELISIATPGQVSEVQLNRAK 420
           Y QI +FSAF+SI+NNTGLFGI  +T SDF  QAVD+A  ELI++A P +V +VQLNRAK
Sbjct: 361 YPQIHAFSAFSSIYNNTGLFGIQGTTSSDFGPQAVDVAVKELIAVANPSEVDQVQLNRAK 420

Query: 421 ESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVISSP 480
           ++TKSA+LMNLESRM+ SEDIGRQ+LTYGER PVEHFLKA+D+++  DI ++ QK+ISSP
Sbjct: 421 QATKSAILMNLESRMVASEDIGRQLLTYGERNPVEHFLKAIDAVSAKDIASVVQKLISSP 480

Query: 481 LTMGSYGDVINVPTYESVNSKFHRK 506
           LTM SYGDV+++P+Y++V+S+F  K
Sbjct: 481 LTMASYGDVLSLPSYDAVSSRFRSK 504

BLAST of Spg025018 vs. ExPASy Swiss-Prot
Match: O04308 (Probable mitochondrial-processing peptidase subunit alpha-2, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MPPalpha2 PE=1 SV=1)

HSP 1 Score: 670.2 bits (1728), Expect = 1.8e-191
Identity = 341/505 (67.52%), Postives = 410/505 (81.19%), Query Frame = 0

Query: 1   MYRIAGSRLRSLK---NHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLQTP 60
           MYR A SR ++LK   NH  N  ASR+A+SSAVA   TS    SWL+G  SSSLP +  P
Sbjct: 1   MYRTAASRAKALKGILNH--NFRASRYASSSAVA---TSSSSSSWLSGGYSSSLPSMNIP 60

Query: 61  LSGITLPPPLPDHVEPSKTKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETPVSSGA 120
           L+G++LPPPL DHVEPSK K TTLPNG+ IA+E S NPAASIG+Y+DCGSIYETP   GA
Sbjct: 61  LAGVSLPPPLSDHVEPSKLKTTTLPNGLTIATEMSPNPAASIGLYVDCGSIYETPQFRGA 120

Query: 121 SHLLERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVD 180
           +HLLER+AFKST NRSH R+VRE+EAIGGNT ASASREQMGYT DALKTYVPEMVE+L+D
Sbjct: 121 THLLERMAFKSTLNRSHFRLVREIEAIGGNTSASASREQMGYTIDALKTYVPEMVEVLID 180

Query: 181 CVRNPVFLDWEINEELQKLKAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSR 240
            VRNP FLDWE+NEEL+K+K E+GE + NP G LLEA+HSAGYSGALANPL APE A++ 
Sbjct: 181 SVRNPAFLDWEVNEELRKVKVEIGEFATNPMGFLLEAVHSAGYSGALANPLYAPESAITG 240

Query: 241 LDGTILEEFIAENYTAPRMVLAASGVEHEELLSIAEPLLSDLPKVPSPKVPESAYVGGDY 300
           L G +LE F+ ENYTA RMVLAASGV+HEELL + EPLLSDLP VP P  P+S YVGGD+
Sbjct: 241 LTGEVLENFVFENYTASRMVLAASGVDHEELLKVVEPLLSDLPNVPRPAEPKSQYVGGDF 300

Query: 301 RRQADCGSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRV 360
           R+     +TH ALAFEVP GW+ EK+AI+ TVLQMLMGGGGSFSAGGPGKGMHS LYLR+
Sbjct: 301 RQHTGGEATHFALAFEVP-GWNNEKEAIIATVLQMLMGGGGSFSAGGPGKGMHSWLYLRL 360

Query: 361 LNEYQQIQSFSAFNSIFNNTGLFGIYASTGSDFVAQAVDIAASELISIATPGQVSEVQLN 420
           LN++QQ QS +AF S+FNNTGLFGIY  T  +F +Q +++ ASE+ ++A  G+V++  L+
Sbjct: 361 LNQHQQFQSCTAFTSVFNNTGLFGIYGCTSPEFASQGIELVASEMNAVA-DGKVNQKHLD 420

Query: 421 RAKESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVI 480
           RAK +TKSA+LMNLESRMI +EDIGRQILTYGERKPV+ FLK VD +TL DI +   KVI
Sbjct: 421 RAKAATKSAILMNLESRMIAAEDIGRQILTYGERKPVDQFLKTVDQLTLKDIADFTSKVI 480

Query: 481 SSPLTMGSYGDVINVPTYESVNSKF 503
           + PLTM ++GDV+NVP+Y+SV+ +F
Sbjct: 481 TKPLTMATFGDVLNVPSYDSVSKRF 498

BLAST of Spg025018 vs. ExPASy Swiss-Prot
Match: Q9ZU25 (Probable mitochondrial-processing peptidase subunit alpha-1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MPPalpha1 PE=1 SV=1)

HSP 1 Score: 653.7 bits (1685), Expect = 1.7e-186
Identity = 335/504 (66.47%), Postives = 406/504 (80.56%), Query Frame = 0

Query: 1   MYRIAGSRLRSLKN-HAANLGASRFATSSAVAARPTS-GGFFSWLTGERSSSLPPLQTPL 60
           MYR A SR R+LK     +L  +R+A+SSAVA   +S   + SWL+G   ++L  L  PL
Sbjct: 1   MYRTAASRARALKGVLTRSLRPARYASSSAVAETSSSTPAYLSWLSGGSRAALTSLDMPL 60

Query: 61  SGITLPPPLPDHVEPSKTKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETPVSSGAS 120
            G++LPPPL D VEPSK +ITTLPNG++IASET+ NPAASIG+Y+DCGSIYE P   GA+
Sbjct: 61  QGVSLPPPLADKVEPSKLQITTLPNGLKIASETTPNPAASIGLYVDCGSIYEAPYFHGAT 120

Query: 121 HLLERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVDC 180
           HLLER+AFKST NR+H R+VRE+EAIGGNT ASASREQM YT DALKTYVPEMVE+L+D 
Sbjct: 121 HLLERMAFKSTLNRTHFRLVREIEAIGGNTSASASREQMSYTIDALKTYVPEMVEVLIDS 180

Query: 181 VRNPVFLDWEINEELQKLKAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRL 240
           VRNP FLDWE+NEEL+K+K E+ EL+ NP G LLEAIHSAGYSG LA+PL APE AL RL
Sbjct: 181 VRNPAFLDWEVNEELRKMKVEIAELAKNPMGFLLEAIHSAGYSGPLASPLYAPESALDRL 240

Query: 241 DGTILEEFIAENYTAPRMVLAASGVEHEELLSIAEPLLSDLPKVPSPKVPESAYVGGDYR 300
           +G +LEEF+ EN+TA RMVLAASGVEHEELL +AEPL SDLP VP    P+S YVGGD+R
Sbjct: 241 NGELLEEFMTENFTAARMVLAASGVEHEELLKVAEPLTSDLPNVPPQLAPKSQYVGGDFR 300

Query: 301 RQADCGSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVL 360
           +     +TH A+AFEVP GW+ EK+A+  TVLQMLMGGGGSFSAGGPGKGMHS LY RVL
Sbjct: 301 QHTGGEATHFAVAFEVP-GWNNEKEAVTATVLQMLMGGGGSFSAGGPGKGMHSWLYRRVL 360

Query: 361 NEYQQIQSFSAFNSIFNNTGLFGIYASTGSDFVAQAVDIAASELISIATPGQVSEVQLNR 420
           NEYQ++QS +AF SIFN+TGLFGIY  +   F A+A+++AA EL  +A  G+V++  L+R
Sbjct: 361 NEYQEVQSCTAFTSIFNDTGLFGIYGCSSPQFAAKAIELAAKELKDVA-GGKVNQAHLDR 420

Query: 421 AKESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVIS 480
           AK +TKSAVLMNLESRMI +EDIGRQILTYGERKPV+ FLK+VD +TL DI +   KVIS
Sbjct: 421 AKAATKSAVLMNLESRMIAAEDIGRQILTYGERKPVDQFLKSVDQLTLKDIADFTSKVIS 480

Query: 481 SPLTMGSYGDVINVPTYESVNSKF 503
            PLTMGS+GDV+ VP+Y++++SKF
Sbjct: 481 KPLTMGSFGDVLAVPSYDTISSKF 502

BLAST of Spg025018 vs. ExPASy Swiss-Prot
Match: P97997 (Mitochondrial-processing peptidase subunit alpha OS=Blastocladiella emersonii OX=4808 PE=3 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 9.8e-65
Identity = 147/428 (34.35%), Postives = 234/428 (54.67%), Query Frame = 0

Query: 76  TKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETPVSSGASHLLERLAFKSTTNRSHL 135
           T +T LP+G+R+A+  S++  A++G+Y+D G IYET +  G SH +  LAFKST   +  
Sbjct: 15  TCMTRLPSGIRVATAPSNSHFAAVGVYVDAGPIYETSIDRGVSHFVSSLAFKSTHGATES 74

Query: 136 RIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVDCVRNPVFLDWEINEELQK 195
           ++++ +  +GGN   +A+RE + Y    L   +P  V+LL D    P   + EI E    
Sbjct: 75  QVLKTMAGLGGNLFCTATRESILYQGSVLHHDLPRTVQLLADTTLRPALTEEEIAERRAT 134

Query: 196 LKAELGELSNNPQGLLLEAIHSAGYSG-ALANPLVAPEFALSRLDGTILEEFIAENYTAP 255
           +  E  +L + P   + E +H+  + G  L N +         +    + E+ A      
Sbjct: 135 IAFEAEDLHSRPDAFIGEMMHAVAFGGRGLGNSIFCEPQRARNMTSDTIREYFATYLHPS 194

Query: 256 RMVLAASGVEHEELLSIAE----PLLSDLPKVPSPKVPESAYVGGDYR----------RQ 315
           RMV+A +GV H EL+ +      P  +  P   +    E+AYVGG ++            
Sbjct: 195 RMVVAGTGVAHAELVDLVSKAFVPSSTRAPSSVTHSDIETAYVGGSHQLVIPKPPPTHPN 254

Query: 316 ADCGSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNE 375
            +   THV +AF VP   H   D   ++ LQ+LMGGGG+FSAGGPGKGM+SRLY  VLN 
Sbjct: 255 YEQTLTHVQVAFPVPPFTH--PDMFPVSTLQVLMGGGGAFSAGGPGKGMYSRLYTNVLNR 314

Query: 376 YQQIQSFSAFNSIFNNTGLFGIYASTGSDFVAQAVDIAASELISIATPGQVSEVQLNRAK 435
           Y+ ++S +AF   +++T LFGI AS    F     ++ A E + +A    +S+ ++ RAK
Sbjct: 315 YRWMESCAAFQHAYSSTSLFGISASCVPSFNPHLCNVLAGEFVHMAR--NLSDEEVARAK 374

Query: 436 ESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVISSP 489
              KS++LMNLES++I  EDIGRQ+L   +R      +  + ++T +D+  +A+ +++ P
Sbjct: 375 NQLKSSLLMNLESQVITVEDIGRQVLAQNQRLEPLELVNNISAVTRDDLVRVAEALVAKP 434

BLAST of Spg025018 vs. ExPASy Swiss-Prot
Match: P23955 (Mitochondrial-processing peptidase subunit alpha OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=mpp PE=1 SV=2)

HSP 1 Score: 239.6 bits (610), Expect = 7.8e-62
Identity = 157/485 (32.37%), Postives = 244/485 (50.31%), Query Frame = 0

Query: 72  EPS-KTKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETPVSSGASHLLERLAFKSTT 131
           EP+ +  ITTL NGVR+ASE   +  + +G+Y+D GS YE     GASH+++RLAFKST+
Sbjct: 47  EPTERDNITTLSNGVRVASEDLPDAFSGVGVYIDAGSRYENDYVRGASHIMDRLAFKSTS 106

Query: 132 NRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVDCVRNPVFLDWEIN 191
            R+   ++  VE +GGN   ++SRE M Y        +P  VEL+ + +R+P   D E+ 
Sbjct: 107 TRTADEMLETVEKLGGNIQCASSRESMMYQAATFNKAIPTAVELMAETIRDPKLTDEELE 166

Query: 192 EELQKLKAELGELSNNPQGLLLEAIHSAGY-SGALANPLVAPEFALSRLDGTILEEFIAE 251
            ++   + E+ E+ +  + +L E +H A +    L NPL+ P+  L  ++  +++ +   
Sbjct: 167 GQIMTAQYEVNEIWSKAELILPELVHMAAFKDNTLGNPLLCPKERLDYINRDVIQTYRDA 226

Query: 252 NYTAPRMVLAASGVEHEELLSIAEPLLSD------------------------------- 311
            Y   R+V+A +GV HE  + +AE    D                               
Sbjct: 227 FYRPERLVVAFAGVPHERAVKLAEKYFGDMKASDAPGLSRTGSETSVDSLVSESSEASSE 286

Query: 312 ---------------LPKVPSPK------------VPESA--------YVGGDYRRQADC 371
                          L K+ SPK            VP S         Y GG     +  
Sbjct: 287 SSSSSSDSSESSGGLLSKLFSPKAKKATPNPFLTRVPISTEDLTRPAHYTGGFLTLPSQP 346

Query: 372 GS--------THVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYL 431
                     TH+ LAFE  G    + D   L  LQ L+GGGGSFSAGGPGKGM+SRLY 
Sbjct: 347 PPLNPNLPTFTHIQLAFE--GLAISDDDIYALATLQTLLGGGGSFSAGGPGKGMYSRLYT 406

Query: 432 RVLNEYQQIQSFSAFNSIFNNTGLFGIYASTGSDFVAQAVDIAASELISIATP---GQVS 478
            VLN++  ++S  AFN  + ++GLFGI AS         + +   EL ++ T      + 
Sbjct: 407 NVLNQHGWVESCVAFNHSYTDSGLFGIAASCYPGRTLPMLQVMCRELHALTTDHGYSALG 466

BLAST of Spg025018 vs. ExPASy TrEMBL
Match: A0A6J1GAE8 (Alpha-MPP OS=Cucurbita moschata OX=3662 GN=LOC111452402 PE=3 SV=1)

HSP 1 Score: 948.3 bits (2450), Expect = 1.3e-272
Identity = 490/505 (97.03%), Postives = 497/505 (98.42%), Query Frame = 0

Query: 1   MYRIAGSRLRSLKNHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLQTPLSG 60
           M+RIAGSRLR LKNHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPL+TPLS 
Sbjct: 1   MHRIAGSRLRLLKNHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLETPLSD 60

Query: 61  ITLPPPLPDHVEPSKTKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETPVSSGASHL 120
           ITLPPPLPDHVEPSKTKITTLPNG RIASETSSNPAASIGIYLDCGSIYETP SSGASHL
Sbjct: 61  ITLPPPLPDHVEPSKTKITTLPNGARIASETSSNPAASIGIYLDCGSIYETPSSSGASHL 120

Query: 121 LERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVDCVR 180
           LERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVDCVR
Sbjct: 121 LERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVDCVR 180

Query: 181 NPVFLDWEINEELQKLKAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDG 240
           NPVFLDWEI EELQKLKAE+GELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDG
Sbjct: 181 NPVFLDWEIKEELQKLKAEIGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDG 240

Query: 241 TILEEFIAENYTAPRMVLAASGVEHEELLSIAEPLLSDLPKVPSPKVPESAYVGGDYRRQ 300
           TILEEFIAENYTAPRMVLAASGVEHEELLSIAEPLLSDLPKVPS KVPESAYVGGDYRRQ
Sbjct: 241 TILEEFIAENYTAPRMVLAASGVEHEELLSIAEPLLSDLPKVPSLKVPESAYVGGDYRRQ 300

Query: 301 ADCGSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNE 360
           ADCGSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNE
Sbjct: 301 ADCGSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNE 360

Query: 361 YQQIQSFSAFNSIFNNTGLFGIYASTGSDFVAQAVDIAASELISIATPGQVSEVQLNRAK 420
           YQQIQSFSAFNSIFN+TGLFGIYASTGS+FVAQAVDIAASELISIATPGQVS+VQLNRAK
Sbjct: 361 YQQIQSFSAFNSIFNDTGLFGIYASTGSNFVAQAVDIAASELISIATPGQVSQVQLNRAK 420

Query: 421 ESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVISSP 480
           ESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVISSP
Sbjct: 421 ESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVISSP 480

Query: 481 LTMGSYGDVINVPTYESVNSKFHRK 506
           LTM SYGDVINVPTYESVNS+FH K
Sbjct: 481 LTMASYGDVINVPTYESVNSRFHSK 505

BLAST of Spg025018 vs. ExPASy TrEMBL
Match: A0A5A7U8Y8 (Alpha-MPP OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold323G00220 PE=3 SV=1)

HSP 1 Score: 947.2 bits (2447), Expect = 2.8e-272
Identity = 486/505 (96.24%), Postives = 497/505 (98.42%), Query Frame = 0

Query: 1   MYRIAGSRLRSLKNHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLQTPLSG 60
           M+RIAGSRLRSLKNHAANLGASRFATSSAVA RPTSGGFFSWLTGERSSSLPP++ PLSG
Sbjct: 1   MHRIAGSRLRSLKNHAANLGASRFATSSAVATRPTSGGFFSWLTGERSSSLPPMEIPLSG 60

Query: 61  ITLPPPLPDHVEPSKTKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETPVSSGASHL 120
           I LPPPLPDHVEPSKTKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETP+SSGASHL
Sbjct: 61  INLPPPLPDHVEPSKTKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETPLSSGASHL 120

Query: 121 LERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVDCVR 180
           LERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVDCVR
Sbjct: 121 LERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVDCVR 180

Query: 181 NPVFLDWEINEELQKLKAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDG 240
           NPVFLDWEINEELQKLKAE+GEL+NNPQGLLLEA+HSAGYSGALANPLVAPEFALSRLDG
Sbjct: 181 NPVFLDWEINEELQKLKAEIGELANNPQGLLLEAVHSAGYSGALANPLVAPEFALSRLDG 240

Query: 241 TILEEFIAENYTAPRMVLAASGVEHEELLSIAEPLLSDLPKVPSPKVPESAYVGGDYRRQ 300
           TILEEFIAENYTAPRMVLAASGV+HEELLSIAEPLLSDLPKVP  KVPESAYVGGDYRRQ
Sbjct: 241 TILEEFIAENYTAPRMVLAASGVDHEELLSIAEPLLSDLPKVPGVKVPESAYVGGDYRRQ 300

Query: 301 ADCGSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNE 360
           ADCGSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNE
Sbjct: 301 ADCGSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNE 360

Query: 361 YQQIQSFSAFNSIFNNTGLFGIYASTGSDFVAQAVDIAASELISIATPGQVSEVQLNRAK 420
           YQQIQSFSAFNSIFNNTGLFGIYA TGS+FVA+AVDIAASELISIATPGQVS+VQLNRAK
Sbjct: 361 YQQIQSFSAFNSIFNNTGLFGIYACTGSNFVAKAVDIAASELISIATPGQVSQVQLNRAK 420

Query: 421 ESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVISSP 480
           ESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVISSP
Sbjct: 421 ESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVISSP 480

Query: 481 LTMGSYGDVINVPTYESVNSKFHRK 506
           LTM SYGDVINVPTYESVNS FHRK
Sbjct: 481 LTMASYGDVINVPTYESVNSLFHRK 505

BLAST of Spg025018 vs. ExPASy TrEMBL
Match: A0A6J1IRH2 (Alpha-MPP OS=Cucurbita maxima OX=3661 GN=LOC111479857 PE=3 SV=1)

HSP 1 Score: 945.3 bits (2442), Expect = 1.1e-271
Identity = 487/503 (96.82%), Postives = 496/503 (98.61%), Query Frame = 0

Query: 1   MYRIAGSRLRSLKNHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLQTPLSG 60
           M+RIAGSRLRSLKNHAANLGASRFATS AVAARPTSGGFFSWLTGERSSSLPPL+TPLSG
Sbjct: 1   MHRIAGSRLRSLKNHAANLGASRFATSGAVAARPTSGGFFSWLTGERSSSLPPLETPLSG 60

Query: 61  ITLPPPLPDHVEPSKTKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETPVSSGASHL 120
           ITLPPPLPD+VEPSKTKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETPVSSGASHL
Sbjct: 61  ITLPPPLPDYVEPSKTKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETPVSSGASHL 120

Query: 121 LERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVDCVR 180
           LERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTY PEMVELLVDCVR
Sbjct: 121 LERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTYSPEMVELLVDCVR 180

Query: 181 NPVFLDWEINEELQKLKAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDG 240
           NPVFLDWEI+EELQKLKAE+ ELSNNPQGLLLEAIHSAGYSGALANPLVAPE+ALSRLDG
Sbjct: 181 NPVFLDWEIDEELQKLKAEIAELSNNPQGLLLEAIHSAGYSGALANPLVAPEYALSRLDG 240

Query: 241 TILEEFIAENYTAPRMVLAASGVEHEELLSIAEPLLSDLPKVPSPKVPESAYVGGDYRRQ 300
           TILEEFIA+NYTAPRMVLAASGVEHEELLSIAEPLLSDLPKVPSPKVPESAYVGGDYRRQ
Sbjct: 241 TILEEFIADNYTAPRMVLAASGVEHEELLSIAEPLLSDLPKVPSPKVPESAYVGGDYRRQ 300

Query: 301 ADCGSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNE 360
           AD GSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNE
Sbjct: 301 ADSGSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNE 360

Query: 361 YQQIQSFSAFNSIFNNTGLFGIYASTGSDFVAQAVDIAASELISIATPGQVSEVQLNRAK 420
           YQQIQSFSAFNSIFNNTGLFGIYASTGSDFVA AVDIAASELISIATPGQVSEVQLNRAK
Sbjct: 361 YQQIQSFSAFNSIFNNTGLFGIYASTGSDFVAHAVDIAASELISIATPGQVSEVQLNRAK 420

Query: 421 ESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVISSP 480
           ESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKV+SSP
Sbjct: 421 ESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVMSSP 480

Query: 481 LTMGSYGDVINVPTYESVNSKFH 504
           LTM SYGDVINVPTYESVN +FH
Sbjct: 481 LTMASYGDVINVPTYESVNRRFH 503

BLAST of Spg025018 vs. ExPASy TrEMBL
Match: A0A6J1GUC8 (Alpha-MPP OS=Cucurbita moschata OX=3662 GN=LOC111457624 PE=3 SV=1)

HSP 1 Score: 944.5 bits (2440), Expect = 1.8e-271
Identity = 486/503 (96.62%), Postives = 495/503 (98.41%), Query Frame = 0

Query: 1   MYRIAGSRLRSLKNHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLQTPLSG 60
           M+RIAGSRLRSLKNHAANLGASRFATS AVAARPTSGGFFSWLTGERSSS PPL+ PLSG
Sbjct: 1   MHRIAGSRLRSLKNHAANLGASRFATSGAVAARPTSGGFFSWLTGERSSSPPPLEIPLSG 60

Query: 61  ITLPPPLPDHVEPSKTKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETPVSSGASHL 120
           ITLPPPLPD+VEPSKTKI+TLPNGVRIASETSSNPAASIGIYLDCGSIYETPVSSGASHL
Sbjct: 61  ITLPPPLPDYVEPSKTKISTLPNGVRIASETSSNPAASIGIYLDCGSIYETPVSSGASHL 120

Query: 121 LERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVDCVR 180
           LERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTY PEMVELLVDCVR
Sbjct: 121 LERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTYSPEMVELLVDCVR 180

Query: 181 NPVFLDWEINEELQKLKAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDG 240
           NPVFLDWEINEELQKLKAE+ ELSNNPQGLLLEAIHSAGYSGALANPLVAPE+ALSRLDG
Sbjct: 181 NPVFLDWEINEELQKLKAEIAELSNNPQGLLLEAIHSAGYSGALANPLVAPEYALSRLDG 240

Query: 241 TILEEFIAENYTAPRMVLAASGVEHEELLSIAEPLLSDLPKVPSPKVPESAYVGGDYRRQ 300
           TILEEFIA+NYTAPRMVLAASGVEHEELLSIAEPLLSDLPKVPSPKVPESAYVGGDYRRQ
Sbjct: 241 TILEEFIADNYTAPRMVLAASGVEHEELLSIAEPLLSDLPKVPSPKVPESAYVGGDYRRQ 300

Query: 301 ADCGSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNE 360
           ADCGSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNE
Sbjct: 301 ADCGSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNE 360

Query: 361 YQQIQSFSAFNSIFNNTGLFGIYASTGSDFVAQAVDIAASELISIATPGQVSEVQLNRAK 420
           YQQIQSFSAFNSIFNNTGLFGIYASTGSDFVA AVDIAASELISIATPGQVSEVQLNRAK
Sbjct: 361 YQQIQSFSAFNSIFNNTGLFGIYASTGSDFVAHAVDIAASELISIATPGQVSEVQLNRAK 420

Query: 421 ESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVISSP 480
           ESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKV+SSP
Sbjct: 421 ESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVMSSP 480

Query: 481 LTMGSYGDVINVPTYESVNSKFH 504
           LTM SYGDVINVPTYESVN +FH
Sbjct: 481 LTMASYGDVINVPTYESVNRRFH 503

BLAST of Spg025018 vs. ExPASy TrEMBL
Match: A0A1S3BIC6 (Alpha-MPP OS=Cucumis melo OX=3656 GN=LOC103489980 PE=3 SV=1)

HSP 1 Score: 944.5 bits (2440), Expect = 1.8e-271
Identity = 484/505 (95.84%), Postives = 496/505 (98.22%), Query Frame = 0

Query: 1   MYRIAGSRLRSLKNHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLQTPLSG 60
           M+RIAGSRLRSLKNHAANLGASRFATS+AVA RPTSGGFFSWLTGERSSSLPP++ PLSG
Sbjct: 1   MHRIAGSRLRSLKNHAANLGASRFATSTAVATRPTSGGFFSWLTGERSSSLPPMEIPLSG 60

Query: 61  ITLPPPLPDHVEPSKTKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETPVSSGASHL 120
           I LPPPLPDHVEPSKTKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETP+SSGASHL
Sbjct: 61  INLPPPLPDHVEPSKTKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETPLSSGASHL 120

Query: 121 LERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVDCVR 180
           LERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVDCVR
Sbjct: 121 LERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVDCVR 180

Query: 181 NPVFLDWEINEELQKLKAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRLDG 240
           NPVFLDWEINEELQKLKAE+GEL+NNPQGLLLEA+HSAGYSGALANPLVAPEFALSRLDG
Sbjct: 181 NPVFLDWEINEELQKLKAEIGELANNPQGLLLEAVHSAGYSGALANPLVAPEFALSRLDG 240

Query: 241 TILEEFIAENYTAPRMVLAASGVEHEELLSIAEPLLSDLPKVPSPKVPESAYVGGDYRRQ 300
           TILEEFIAENYTAPRMVLAASGV+HEELLSIAEPLLSDLPKVP  KVPESAYVGGDYRRQ
Sbjct: 241 TILEEFIAENYTAPRMVLAASGVDHEELLSIAEPLLSDLPKVPGVKVPESAYVGGDYRRQ 300

Query: 301 ADCGSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNE 360
           ADCGSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNE
Sbjct: 301 ADCGSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVLNE 360

Query: 361 YQQIQSFSAFNSIFNNTGLFGIYASTGSDFVAQAVDIAASELISIATPGQVSEVQLNRAK 420
           YQQIQSFSAFNSIFNNTGLFGIYA TGS+FVA+AVDIAASELISIATPGQVS+VQLNRAK
Sbjct: 361 YQQIQSFSAFNSIFNNTGLFGIYACTGSNFVAKAVDIAASELISIATPGQVSQVQLNRAK 420

Query: 421 ESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVISSP 480
           ESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVISSP
Sbjct: 421 ESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVISSP 480

Query: 481 LTMGSYGDVINVPTYESVNSKFHRK 506
           LTM SYGDVINVPTYESVN  FHRK
Sbjct: 481 LTMASYGDVINVPTYESVNGLFHRK 505

BLAST of Spg025018 vs. TAIR 10
Match: AT3G16480.1 (mitochondrial processing peptidase alpha subunit )

HSP 1 Score: 670.2 bits (1728), Expect = 1.3e-192
Identity = 341/505 (67.52%), Postives = 410/505 (81.19%), Query Frame = 0

Query: 1   MYRIAGSRLRSLK---NHAANLGASRFATSSAVAARPTSGGFFSWLTGERSSSLPPLQTP 60
           MYR A SR ++LK   NH  N  ASR+A+SSAVA   TS    SWL+G  SSSLP +  P
Sbjct: 1   MYRTAASRAKALKGILNH--NFRASRYASSSAVA---TSSSSSSWLSGGYSSSLPSMNIP 60

Query: 61  LSGITLPPPLPDHVEPSKTKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETPVSSGA 120
           L+G++LPPPL DHVEPSK K TTLPNG+ IA+E S NPAASIG+Y+DCGSIYETP   GA
Sbjct: 61  LAGVSLPPPLSDHVEPSKLKTTTLPNGLTIATEMSPNPAASIGLYVDCGSIYETPQFRGA 120

Query: 121 SHLLERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVD 180
           +HLLER+AFKST NRSH R+VRE+EAIGGNT ASASREQMGYT DALKTYVPEMVE+L+D
Sbjct: 121 THLLERMAFKSTLNRSHFRLVREIEAIGGNTSASASREQMGYTIDALKTYVPEMVEVLID 180

Query: 181 CVRNPVFLDWEINEELQKLKAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSR 240
            VRNP FLDWE+NEEL+K+K E+GE + NP G LLEA+HSAGYSGALANPL APE A++ 
Sbjct: 181 SVRNPAFLDWEVNEELRKVKVEIGEFATNPMGFLLEAVHSAGYSGALANPLYAPESAITG 240

Query: 241 LDGTILEEFIAENYTAPRMVLAASGVEHEELLSIAEPLLSDLPKVPSPKVPESAYVGGDY 300
           L G +LE F+ ENYTA RMVLAASGV+HEELL + EPLLSDLP VP P  P+S YVGGD+
Sbjct: 241 LTGEVLENFVFENYTASRMVLAASGVDHEELLKVVEPLLSDLPNVPRPAEPKSQYVGGDF 300

Query: 301 RRQADCGSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRV 360
           R+     +TH ALAFEVP GW+ EK+AI+ TVLQMLMGGGGSFSAGGPGKGMHS LYLR+
Sbjct: 301 RQHTGGEATHFALAFEVP-GWNNEKEAIIATVLQMLMGGGGSFSAGGPGKGMHSWLYLRL 360

Query: 361 LNEYQQIQSFSAFNSIFNNTGLFGIYASTGSDFVAQAVDIAASELISIATPGQVSEVQLN 420
           LN++QQ QS +AF S+FNNTGLFGIY  T  +F +Q +++ ASE+ ++A  G+V++  L+
Sbjct: 361 LNQHQQFQSCTAFTSVFNNTGLFGIYGCTSPEFASQGIELVASEMNAVA-DGKVNQKHLD 420

Query: 421 RAKESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVI 480
           RAK +TKSA+LMNLESRMI +EDIGRQILTYGERKPV+ FLK VD +TL DI +   KVI
Sbjct: 421 RAKAATKSAILMNLESRMIAAEDIGRQILTYGERKPVDQFLKTVDQLTLKDIADFTSKVI 480

Query: 481 SSPLTMGSYGDVINVPTYESVNSKF 503
           + PLTM ++GDV+NVP+Y+SV+ +F
Sbjct: 481 TKPLTMATFGDVLNVPSYDSVSKRF 498

BLAST of Spg025018 vs. TAIR 10
Match: AT1G51980.1 (Insulinase (Peptidase family M16) protein )

HSP 1 Score: 653.7 bits (1685), Expect = 1.2e-187
Identity = 335/504 (66.47%), Postives = 406/504 (80.56%), Query Frame = 0

Query: 1   MYRIAGSRLRSLKN-HAANLGASRFATSSAVAARPTS-GGFFSWLTGERSSSLPPLQTPL 60
           MYR A SR R+LK     +L  +R+A+SSAVA   +S   + SWL+G   ++L  L  PL
Sbjct: 1   MYRTAASRARALKGVLTRSLRPARYASSSAVAETSSSTPAYLSWLSGGSRAALTSLDMPL 60

Query: 61  SGITLPPPLPDHVEPSKTKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETPVSSGAS 120
            G++LPPPL D VEPSK +ITTLPNG++IASET+ NPAASIG+Y+DCGSIYE P   GA+
Sbjct: 61  QGVSLPPPLADKVEPSKLQITTLPNGLKIASETTPNPAASIGLYVDCGSIYEAPYFHGAT 120

Query: 121 HLLERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVDC 180
           HLLER+AFKST NR+H R+VRE+EAIGGNT ASASREQM YT DALKTYVPEMVE+L+D 
Sbjct: 121 HLLERMAFKSTLNRTHFRLVREIEAIGGNTSASASREQMSYTIDALKTYVPEMVEVLIDS 180

Query: 181 VRNPVFLDWEINEELQKLKAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRL 240
           VRNP FLDWE+NEEL+K+K E+ EL+ NP G LLEAIHSAGYSG LA+PL APE AL RL
Sbjct: 181 VRNPAFLDWEVNEELRKMKVEIAELAKNPMGFLLEAIHSAGYSGPLASPLYAPESALDRL 240

Query: 241 DGTILEEFIAENYTAPRMVLAASGVEHEELLSIAEPLLSDLPKVPSPKVPESAYVGGDYR 300
           +G +LEEF+ EN+TA RMVLAASGVEHEELL +AEPL SDLP VP    P+S YVGGD+R
Sbjct: 241 NGELLEEFMTENFTAARMVLAASGVEHEELLKVAEPLTSDLPNVPPQLAPKSQYVGGDFR 300

Query: 301 RQADCGSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVL 360
           +     +TH A+AFEVP GW+ EK+A+  TVLQMLMGGGGSFSAGGPGKGMHS LY RVL
Sbjct: 301 QHTGGEATHFAVAFEVP-GWNNEKEAVTATVLQMLMGGGGSFSAGGPGKGMHSWLYRRVL 360

Query: 361 NEYQQIQSFSAFNSIFNNTGLFGIYASTGSDFVAQAVDIAASELISIATPGQVSEVQLNR 420
           NEYQ++QS +AF SIFN+TGLFGIY  +   F A+A+++AA EL  +A  G+V++  L+R
Sbjct: 361 NEYQEVQSCTAFTSIFNDTGLFGIYGCSSPQFAAKAIELAAKELKDVA-GGKVNQAHLDR 420

Query: 421 AKESTKSAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVIS 480
           AK +TKSAVLMNLESRMI +EDIGRQILTYGERKPV+ FLK+VD +TL DI +   KVIS
Sbjct: 421 AKAATKSAVLMNLESRMIAAEDIGRQILTYGERKPVDQFLKSVDQLTLKDIADFTSKVIS 480

Query: 481 SPLTMGSYGDVINVPTYESVNSKF 503
            PLTMGS+GDV+ VP+Y++++SKF
Sbjct: 481 KPLTMGSFGDVLAVPSYDTISSKF 502

BLAST of Spg025018 vs. TAIR 10
Match: AT1G51980.2 (Insulinase (Peptidase family M16) protein )

HSP 1 Score: 584.7 bits (1506), Expect = 7.0e-167
Identity = 303/452 (67.04%), Postives = 363/452 (80.31%), Query Frame = 0

Query: 1   MYRIAGSRLRSLKN-HAANLGASRFATSSAVAARPTS-GGFFSWLTGERSSSLPPLQTPL 60
           MYR A SR R+LK     +L  +R+A+SSAVA   +S   + SWL+G   ++L  L  PL
Sbjct: 1   MYRTAASRARALKGVLTRSLRPARYASSSAVAETSSSTPAYLSWLSGGSRAALTSLDMPL 60

Query: 61  SGITLPPPLPDHVEPSKTKITTLPNGVRIASETSSNPAASIGIYLDCGSIYETPVSSGAS 120
            G++LPPPL D VEPSK +ITTLPNG++IASET+ NPAASIG+Y+DCGSIYE P   GA+
Sbjct: 61  QGVSLPPPLADKVEPSKLQITTLPNGLKIASETTPNPAASIGLYVDCGSIYEAPYFHGAT 120

Query: 121 HLLERLAFKSTTNRSHLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVDC 180
           HLLER+AFKST NR+H R+VRE+EAIGGNT ASASREQM YT DALKTYVPEMVE+L+D 
Sbjct: 121 HLLERMAFKSTLNRTHFRLVREIEAIGGNTSASASREQMSYTIDALKTYVPEMVEVLIDS 180

Query: 181 VRNPVFLDWEINEELQKLKAELGELSNNPQGLLLEAIHSAGYSGALANPLVAPEFALSRL 240
           VRNP FLDWE+NEEL+K+K E+ EL+ NP G LLEAIHSAGYSG LA+PL APE AL RL
Sbjct: 181 VRNPAFLDWEVNEELRKMKVEIAELAKNPMGFLLEAIHSAGYSGPLASPLYAPESALDRL 240

Query: 241 DGTILEEFIAENYTAPRMVLAASGVEHEELLSIAEPLLSDLPKVPSPKVPESAYVGGDYR 300
           +G +LEEF+ EN+TA RMVLAASGVEHEELL +AEPL SDLP VP    P+S YVGGD+R
Sbjct: 241 NGELLEEFMTENFTAARMVLAASGVEHEELLKVAEPLTSDLPNVPPQLAPKSQYVGGDFR 300

Query: 301 RQADCGSTHVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRVL 360
           +     +TH A+AFEVP GW+ EK+A+  TVLQMLMGGGGSFSAGGPGKGMHS LY RVL
Sbjct: 301 QHTGGEATHFAVAFEVP-GWNNEKEAVTATVLQMLMGGGGSFSAGGPGKGMHSWLYRRVL 360

Query: 361 NEYQQIQSFSAFNSIFNNTGLFGIYASTGSDFVAQAVDIAASELISIATPGQVSEVQLNR 420
           NEYQ++QS +AF SIFN+TGLFGIY  +   F A+A+++AA EL  +A  G+V++  L+R
Sbjct: 361 NEYQEVQSCTAFTSIFNDTGLFGIYGCSSPQFAAKAIELAAKELKDVA-GGKVNQAHLDR 420

Query: 421 AKESTKSAVLMNLESRMIVSEDIGRQILTYGE 451
           AK +TKSAVLMNLESRMI +EDIGRQILTYGE
Sbjct: 421 AKAATKSAVLMNLESRMIAAEDIGRQILTYGE 450

BLAST of Spg025018 vs. TAIR 10
Match: AT3G02090.1 (Insulinase (Peptidase family M16) protein )

HSP 1 Score: 200.7 bits (509), Expect = 2.9e-51
Identity = 135/442 (30.54%), Postives = 240/442 (54.30%), Query Frame = 0

Query: 75  KTKITTLPNGVRIASETS-SNPAASIGIYLDCGSIYETPVSSGASHLLERLAFKSTTNRS 134
           +T++TTLPNG+R+A+E++ S   A++G+++D GS +E+  ++G +H LE + FK T  R+
Sbjct: 96  ETRVTTLPNGLRVATESNLSAKTATVGVWIDAGSRFESDETNGTAHFLEHMIFKGTDRRT 155

Query: 135 HLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVDCVRNPVFLDWEINEEL 194
              +  E+E IGG+  A  SREQ  Y    L + V + +++L D ++N  F +  IN E 
Sbjct: 156 VRALEEEIEDIGGHLNAYTSREQTTYYAKVLDSNVNQALDVLADILQNSKFEEQRINRER 215

Query: 195 QKLKAELGELSNNPQGLLLEAIHSAGYS-GALANPLVAPEFALSRLDGTILEEFIAENYT 254
             +  E+ E+      ++L+ +H+  +    L   ++ P   +  +    L+ +I  +YT
Sbjct: 216 DVILREMQEVEGQTDEVVLDHLHATAFQYTPLGRTILGPAQNVKSITREDLQNYIKTHYT 275

Query: 255 APRMVLAASG-VEHEELLSIAEPLLSDLPKVPSPKV-----PESAYVGGDYRR-QADCGS 314
           A RMV+AA+G V+HEE++   + L + L   P+          +++ G + R    D   
Sbjct: 276 ASRMVIAAAGAVKHEEVVEQVKKLFTKLSSDPTTTSQLVANEPASFTGSEVRMIDDDLPL 335

Query: 315 THVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRV-LNEYQQI 374
              A+AFE  G    + D++ L V+Q ++G     + GG GK + S L  RV +NE    
Sbjct: 336 AQFAVAFE--GASWTDPDSVALMVMQTMLGSWNK-NVGG-GKHVGSDLTQRVAINEI--A 395

Query: 375 QSFSAFNSIFNNTGLFGIYASTGSDFVAQAVDIAASELISIATPGQVSEVQLNRAKESTK 434
           +S  AFN+ + +TGLFG+YA   +D +         E+  +A   +VS+  + RA+   K
Sbjct: 396 ESIMAFNTNYKDTGLFGVYAVAKADCLDDLSYAIMYEVTKLAY--RVSDADVTRARNQLK 455

Query: 435 SAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVI-SSPLTM 494
           S++L++++    ++EDIGRQ+LTYG R P       +D++  + +  +A K I    + +
Sbjct: 456 SSLLLHMDGTSPIAEDIGRQLLTYGRRIPTAELFARIDAVDASTVKRVANKYIYDKDIAI 515

Query: 495 GSYGDVINVPTYESVNSKFHRK 506
            + G + ++P Y    +KF R+
Sbjct: 516 SAIGPIQDLPDY----NKFRRR 525

BLAST of Spg025018 vs. TAIR 10
Match: AT3G02090.2 (Insulinase (Peptidase family M16) protein )

HSP 1 Score: 195.3 bits (495), Expect = 1.2e-49
Identity = 129/413 (31.23%), Postives = 226/413 (54.72%), Query Frame = 0

Query: 75  KTKITTLPNGVRIASETS-SNPAASIGIYLDCGSIYETPVSSGASHLLERLAFKSTTNRS 134
           +T++TTLPNG+R+A+E++ S   A++G+++D GS +E+  ++G +H LE + FK T  R+
Sbjct: 96  ETRVTTLPNGLRVATESNLSAKTATVGVWIDAGSRFESDETNGTAHFLEHMIFKGTDRRT 155

Query: 135 HLRIVREVEAIGGNTGASASREQMGYTFDALKTYVPEMVELLVDCVRNPVFLDWEINEEL 194
              +  E+E IGG+  A  SREQ  Y    L + V + +++L D ++N  F +  IN E 
Sbjct: 156 VRALEEEIEDIGGHLNAYTSREQTTYYAKVLDSNVNQALDVLADILQNSKFEEQRINRER 215

Query: 195 QKLKAELGELSNNPQGLLLEAIHSAGYS-GALANPLVAPEFALSRLDGTILEEFIAENYT 254
             +  E+ E+      ++L+ +H+  +    L   ++ P   +  +    L+ +I  +YT
Sbjct: 216 DVILREMQEVEGQTDEVVLDHLHATAFQYTPLGRTILGPAQNVKSITREDLQNYIKTHYT 275

Query: 255 APRMVLAASG-VEHEELLSIAEPLLSDLPKVPSPKV-----PESAYVGGDYRR-QADCGS 314
           A RMV+AA+G V+HEE++   + L + L   P+          +++ G + R    D   
Sbjct: 276 ASRMVIAAAGAVKHEEVVEQVKKLFTKLSSDPTTTSQLVANEPASFTGSEVRMIDDDLPL 335

Query: 315 THVALAFEVPGGWHKEKDAIVLTVLQMLMGGGGSFSAGGPGKGMHSRLYLRV-LNEYQQI 374
              A+AFE  G    + D++ L V+Q ++G     + GG GK + S L  RV +NE    
Sbjct: 336 AQFAVAFE--GASWTDPDSVALMVMQTMLGSWNK-NVGG-GKHVGSDLTQRVAINEI--A 395

Query: 375 QSFSAFNSIFNNTGLFGIYASTGSDFVAQAVDIAASELISIATPGQVSEVQLNRAKESTK 434
           +S  AFN+ + +TGLFG+YA   +D +         E+  +A   +VS+  + RA+   K
Sbjct: 396 ESIMAFNTNYKDTGLFGVYAVAKADCLDDLSYAIMYEVTKLAY--RVSDADVTRARNQLK 455

Query: 435 SAVLMNLESRMIVSEDIGRQILTYGERKPVEHFLKAVDSITLNDITNIAQKVI 478
           S++L++++    ++EDIGRQ+LTYG R P       +D++  + +  +A K I
Sbjct: 456 SSLLLHMDGTSPIAEDIGRQLLTYGRRIPTAELFARIDAVDASTVKRVANKYI 500