Spg025013 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg025013
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Malic enzyme
Location	scaffold12: 8358556 .. 8364196 (+)
RNA-Seq Expression	Spg025013
Synteny	Spg025013

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAACCAGGTAGAGAGAGAAACAGATCAGGGGGAAGAGTGAGAGAGAGAGGGATCAGAACAGAACAGATTTAGCAGGAGAAACCGGAGGAGAAAGCTTTCTTCCTTGAGAATCTCATTCTCCTTCCTCCTCTTGTCTTTCTCTCTCTCTCTCTTCACACCCTTACTTAAACAACCCCGTCATTTCTTCTTCTTTCCCACAATCACTTTTCGCCATGATGTCCTTGAACAGAACCACTTTCATGGTATTGCAATTCGAATCCATTGCTTTTATGACCTTCCCAGTCATGCTTTTGTATCTGGGTTCCTTTCAATTTTTGTTGATTTCTCTGATTTCTTCAAAGTTGCTCGTTTTTTTTTCCTCAGACCCAGATGGGTGTTTCTGGAGCTTGCTCCACTCCCTCCGGCACCGGCAGACGGCGGCCGCTGGCTCTGAAAGTGGTGTCCATGGACTCGAATGGCCGAGCCGGCGAGCGAAGCGGCAGTGCTGTGGTGGAGAACAACACTGTCAAAGAAATTGCCAAAGCTCCGTCGCCGGTGGTTGAGGTGGAAACCAAATCCACCGCCGGCACCTGGGCTCAGGATGTGTACGTTGAAGATTCTGCCGCCACGGAGGAACAAATCATGACACCTTGGAGCGTCTCTGTTGCTAGGTGAAGATCACGATCCCCATCTTTCCTATTCATCAGGATTCTTTCTTCTTTTCTTTTTGTTTAGGTTTGAATTGTGTAATTTATTAACTCTCTCTCTCTTTTTTTAACCTTGTGGCATCTTAATCATAAACCTTAATTTAGTTTTTAAATATTATAGGAATTAACTGATTCTATCATATTATATTGGAGTAAAGTCCTTAATATTGGCCTTTTCCTTTTGGGGGGTTATCTTTTTGTATCCTCTTTTTAATATTTACCTTAATTTTATATCTTGTGTTCTTCAACTTGAGTTTTGATGAGTGATGACTGAAGCTGTTATTCCTGATTTGAGCAAATCTTGTTTTGATCAGTGGCTTTGATGGTTTTTTAAACATATCATAGTGATCCAGCTTGCAGTTAGAAAAAACGAAAGGATTGACATAATTTTCCCCTTTTTTGAAAAAAATAAGAATCTTGAAGTCCATTTTGTGTTCATTCAAACTAACCATTTTTATCTGTTCTGTCATCGAAAAGGATCGGCATTAGTTTGGCTTTTGATTGTTTCCTTCGATGCATAACTTCCTCCCTTTATATCATTTCCAATCTAGATCGATCACTTATAAACTTCTGAATTGAATGATGTCTGTTCTTGATAATATTCTAGAGTAATATGTTATTTGTCCGAGTTTACGTTCCGTATAGTTGCTGAATTGTGATTAGCTCTGGCTTATTTGAACTCTGCAAACCTTTGAGAAATTTCTTTTCCAGTTAACATGTTACTTCTATCTCTTTTCTGCTGCCTTTTATTTTAACCGTTCATGGCTGATTTTTTTTTTCTTGTGTTGGTTTTGGCTGTAACCTTAGTGGATATAATTTATTGCGAGATCCACGCCACAACAAAGGGCTTGCGTTTACTGAGAAAGAAAGAGATGCTCACTACTTGCGCGGTCTTCTACCCCCGACAGTGGTTGCTCAAGATCTTCAGGTTATCCTTTCACTTGAGATACTGTTCAGGCCCATTATAAGTTTTACAATCCTAGTTTTTGATTATGAAAACTATTTGAAACTACTTCCAGGTAAAGAAGATGTTGCACAATATCCGTCAATATCAAGTGCCATTGCAGAAGTACATGGCAATGATGGATCTTCAGGTAGCCATTGTTGATCTCATTTTTAGTACTAGGAATTTCAAATGATTCGTTTTCAGATTTTTGGATTTGATGATCTTAATGCGAAATTGTTAACTTCATTCAACTATTGTTTGTTTAATTTGACTTATTTTATGATAAATTCAACAAACTGTTCTCTCTCTCAATTTATACTTTAATGATCAGATTCAGCACACTATATCCTATAACAGACCTTTGCTGTATCCTTTTTCAGGAGAGGAACGAAAAGCTGTTTTACAAGCTCCTTATAAATCATGTAGAAGAATTACTTCCAGTTGTCTATACACCAACCGTGGGTGAAGCTTGCCAGAAATATGGAAGCATCTTCAGGCAACCACAGGGTCTCTATATCAGTTTGAGAGAAAAGTATGACTGATTACGTCGTTTCAGGTTTCTCGACTTGTGATGCATTTCTTTCTTTGTTGTTGTGTTGAAATGGTTGAGTTTCTTTTGCAGGGGAAAGATCCTCGAGGTCTTGCGGAATTGGCCTGAGAAGAACATTCAAGTCATTGTAGTTACTGATGGGGAGAGAATTTTGGGGCTAGGGGATCTTGGATGCCAGGTTAATTCCATTTTCAACTCTTTCTTAAGGAAACTTGTTTAGAAGTATTTCAAATGATGATAGTCCACTGAAGCTTGAGATTGAGCAATGAATTCTTTCTTCTTTGTCTGTCATTGAACATAATAATCCTAACACAGAGCAAATCCATGTTAATGTTTCAGGGGATGGGCATACCCATTGGGAAACTCTCTTTATATACTGCACTTGGGGGTGTTCGTCCTTCAGCGGTGAGTACCTACATGATCTCTTCCTATTCTTCTATATAGTTTCTACTTATCTCAGCATATGCTTGATTTTTCAAATTAACCTTGCTTCATTTCTTTTTCTCGTTGGTTCACTAGTAAATTTTTCGGTTTTTTCAGTGCTTGCCCGTGACCATTGATGTTGGTACAAACAATGAGGAGTTGTTGAATGATGAGTTTTACATTGGGCTCAGGCAAAAGAGAGCTACTGGGCAGGTTTGTGACACCTTCTCCTCTCTCTCCCTCCCCCCCCCCCCCCCCCCCCCCCCGCGATGTATCTTGCATGTCTAGTTCAATCATCAATGTGACAAGTCATACTTATCGCATTCAAGTTTAAGTGGGATGATTTTTCTCAATGTAGGAATATGCTGAACTTATAGATGAATTCATGACTGCGGTCAAACAGTGTTATGGGGAGAAGCTCCTCGTTCAGGTATGTTGCTATATCTACGGTCTACACATATTCTGTGTAGTTAGCAAATCTATTCTTATATATATATATATATTACTCATTCTAATATTTTTCCATTTCAATTTCCACATTTTGAAGTTCGAAGACTTTGCAAACCACAATGCATTCGACTTGCTAGCAAAATACGGCGCAACTCATCTAGTTTTCAATGATGACATTCAGGTTAGAACCTTGGACTTTTCATTTTACTATTTGATTAACTTCAATCAACTTAACTCTAATGGCATTGTCATCGTTGAATCTCTCAGGGGACAGCATCTGTGGTACTTGCTGGGCTCATTGGAGCTTTAAAGTTCGTTGGTGGATCCTTAGCTGACCATAGGTTTTTATTCCTTGGTGCTGGAGAAGTAAGTCCAAATTGAATCATGTCTAACATCTTAAAAAAAGTTCCTCCTGATTTCGGCGTAATATATTAACGTTTGGTGTTGCCTTGTCTGTTTCAGGCCGGTACCGGAATTGCTGAACTCATAGCCCTTGAGATATCAAAACAGGTTAAAATGAATAGTTGTTTTTAGATTACCGATTTTCCTCCTACATTGCTCAGAATTCATTTAGTTAATCTTCATCTTAATTAACAAGAGCTAGTCCTTTGTGTAGACCAATACTCCGTTGGAAGAGACGCGCAAAAAAGTTTGGCTTGTGGATTCCAAGGTAACATATTCATCAAGCTTGAGGAGGTGTTCATCATTCATAAAATCTTCTGAGATTGTATTTCTGATCCCTTTTTTTGTTCTTTCCTCAGGGGCTGATTGTAAGTTCTAGGAAGGATACCCTGCAACATTTCAAGAAACCTTGGGCTCATGAACACAAACCTATTAACAAACTCATCGATGCGGTTAAGGTGTCAAATCTTTCTATTTACAGACAAATGCATATTCATAATTTGAACCAAGTCTTTAAGTTCCTTTATTTACTGTTTCACATCAGGAAATCAAACCAACCGTCTTGATTGGAACATCAGGCGTTGGAAGAACATTCACTAAAGATGTCGTGGAGACTATGGCCTCGATCAATGAGGTATTTAATCCATCTGTTGATTATTTCTTTATTTTTTTACTTTCAGAAGAGTGTATGCTGATCATGACTTTGGGTAACTTGTTGCAGAAACCTATCATTCTCTCTCTTTCCAACCCTACTTCCCAATCTGAGTGTACTGCTGAAGAAGCTTATAAATGGAGCCAGGTAATACAATGATTCAATTTCTTAACTTTCTCAGTTGTATCATAGGGGTGTACCCGAAACCGACTATAGGCTGCAGACTGATTTTGTATGACATTTTTCGGTTGTTGCGTTCAGGGTCGTGCCATTTTCGCAAGTGGAAGCCCATTTGACCCAGTTAAATACAAGGGGAAAGTCTTTGTGCCTGGCCAGGTCCTCACATTTCAATTCAATTCTGTCTAAGCTAAACACATATCATGATTTGGGTGTTAATATTATTCCTTGGTGATTTATTTTTAACAATTCAGGCAAACAATGCATACATCTTTCCTGGATTCGGTTTGGGGTTAATTATGTCAGGCACAATCCGCGTTCATGACGACATGCTCCTAGCAGCCTGTAAGTACTCAAAAGCCTATTAAGATCAAAATAGAGTACGGTATATTAGAAACTGCACTTATTTTCAAAGCTAAGATGGACTAGAGTCCATATCTATGTTTAAATCATCGTTTTTATTGAGTCGGGGTTCGATCCTTGTTTATTGATGTATGGTTTATTACGAGCAGCGGAAGCTTTGGCTGCACAAGTGACCCAAGACGACTTTGACAAAGGACTCATCTATCCTCCATTTACCAACATCAGAAAAATTTCAGCACATATTGCTGCTAGTGTGGCTGCAAAAGCTTATGAACTTGGTGAGTCTTCAACTTCATTTCCCTTTCCCAAACTTTGCATAGTGTTTCAATTTGCTAATCATTCTCTTACCATTTGAAATTCTGGCTTGTCTCATAGGTTTGGCCACTCGCCTCCCTCAACCTAAAGATCTGGTGAAATATGCTGAGAGCTGTATGTACAGTCCTAGCTATCAAAATTATCGGTAGATTAGTGACGTTTCTCTGCTGTAGCTATATGTCGTATCGTTCGTTTATTTTCTGATAATGTATCTTCAATTTATTTTACTTCGTTTTGTCATGTAAGTTATTTGGTGGTTTAGATATCAGCTATAGTTTGTATTAGTTGTGAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAGGAATGAGGGAAAAAAGAACTCTTACAAGTTTTTCATGAAATGGAATGGAAACTTGTGCTCAAAATGTGAAGTGGATTCATGATTTGTTGGTGGAGATTTCTGTTAAATGAAGCAGTAGGCAAAAACTCAGTAATTCACTAAAATTATTTCATAATAACCTGTGCATTTAATGTATATATTGCTTTATATTTTGTAGTAGTGTAGTAAAGCGTGGATACAGACACGTGAAATGGATATGATTCGAAATGGACATGGAAACACGTCATTTTTTTAAATCTAAGACATGGACACGTTTGGTAAAATATACATTTTTTAAAAAATATATATCATTTTTATACTAGAAGGAAATCCAA

mRNA sequence

ATGTATGGTTTATTACGAGCAGCGGAAGCTTTGGCTGCACAAGTGACCCAAGACGACTTTGACAAAGGACTCATCTATCCTCCATTTACCAACATCAGAAAAATTTCAGCACATATTGCTGCTAGTGTGGCTGCAAAAGCTTATGAACTTGGTTTGGCCACTCGCCTCCCTCAACCTAAAGATCTGGTGAAATATGCTGAGAGCTGTATGTACAGTCCTAGCTATCAAAATTATCGGTAG

Coding sequence (CDS)

ATGTATGGTTTATTACGAGCAGCGGAAGCTTTGGCTGCACAAGTGACCCAAGACGACTTTGACAAAGGACTCATCTATCCTCCATTTACCAACATCAGAAAAATTTCAGCACATATTGCTGCTAGTGTGGCTGCAAAAGCTTATGAACTTGGTTTGGCCACTCGCCTCCCTCAACCTAAAGATCTGGTGAAATATGCTGAGAGCTGTATGTACAGTCCTAGCTATCAAAATTATCGGTAG

Protein sequence

MYGLLRAAEALAAQVTQDDFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLVKYAESCMYSPSYQNYR

Homology

[Close]

BLAST of Spg025013 vs. NCBI nr
Match: XP_038900124.1 (NADP-dependent malic enzyme-like [Benincasa hispida])

HSP 1 Score: 147.5 bits (371), Expect = 4.8e-32
Identity = 72/76 (94.74%), Postives = 75/76 (98.68%), Query Frame = 0


Query: 4   LLRAAEALAAQVTQDDFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLV 63
           LL A+EALA+QV+QDDFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLV
Sbjct: 572 LLAASEALASQVSQDDFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLV 631

Query: 64  KYAESCMYSPSYQNYR 80
           KYAESCMYSPSYQNYR
Sbjct: 632 KYAESCMYSPSYQNYR 647

[Close]

BLAST of Spg025013 vs. NCBI nr
Match: XP_022153564.1 (NADP-dependent malic enzyme-like [Momordica charantia])

HSP 1 Score: 146.7 bits (369), Expect = 8.2e-32
Identity = 72/76 (94.74%), Postives = 74/76 (97.37%), Query Frame = 0


Query: 4   LLRAAEALAAQVTQDDFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLV 63
           LL A+EALA+QVTQDDFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDL 
Sbjct: 571 LLAASEALASQVTQDDFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLE 630

Query: 64  KYAESCMYSPSYQNYR 80
           KYAESCMYSPSYQNYR
Sbjct: 631 KYAESCMYSPSYQNYR 646

[Close]

BLAST of Spg025013 vs. NCBI nr
Match: XP_008447551.1 (PREDICTED: NADP-dependent malic enzyme [Cucumis melo] >XP_016900422.1 PREDICTED: NADP-dependent malic enzyme [Cucumis melo])

HSP 1 Score: 146.0 bits (367), Expect = 1.4e-31
Identity = 71/76 (93.42%), Postives = 75/76 (98.68%), Query Frame = 0


Query: 4   LLRAAEALAAQVTQDDFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLV 63
           LL A+EALA+QV+Q+DFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLV
Sbjct: 572 LLAASEALASQVSQEDFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLV 631

Query: 64  KYAESCMYSPSYQNYR 80
           KYAESCMYSPSYQNYR
Sbjct: 632 KYAESCMYSPSYQNYR 647

[Close]

BLAST of Spg025013 vs. NCBI nr
Match: TYK08505.1 (NADP-dependent malic enzyme [Cucumis melo var. makuwa])

HSP 1 Score: 146.0 bits (367), Expect = 1.4e-31
Identity = 71/76 (93.42%), Postives = 75/76 (98.68%), Query Frame = 0


Query: 4   LLRAAEALAAQVTQDDFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLV 63
           LL A+EALA+QV+Q+DFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLV
Sbjct: 560 LLAASEALASQVSQEDFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLV 619

Query: 64  KYAESCMYSPSYQNYR 80
           KYAESCMYSPSYQNYR
Sbjct: 620 KYAESCMYSPSYQNYR 635

[Close]

BLAST of Spg025013 vs. NCBI nr
Match: KAA0050846.1 (NADP-dependent malic enzyme [Cucumis melo var. makuwa])

HSP 1 Score: 146.0 bits (367), Expect = 1.4e-31
Identity = 71/76 (93.42%), Postives = 75/76 (98.68%), Query Frame = 0


Query: 4   LLRAAEALAAQVTQDDFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLV 63
           LL A+EALA+QV+Q+DFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLV
Sbjct: 598 LLAASEALASQVSQEDFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLV 657

Query: 64  KYAESCMYSPSYQNYR 80
           KYAESCMYSPSYQNYR
Sbjct: 658 KYAESCMYSPSYQNYR 673

[Close]

BLAST of Spg025013 vs. ExPASy Swiss-Prot
Match: P51615 (NADP-dependent malic enzyme OS=Vitis vinifera OX=29760 PE=2 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 1.6e-30
Identity = 64/76 (84.21%), Postives = 73/76 (96.05%), Query Frame = 0


Query: 4   LLRAAEALAAQVTQDDFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLV 63
           LL A+EALA QVTQ++FDKGLIYPPF+NIRKISAHIAA+VAAKAYELGLATRLPQP++LV
Sbjct: 516 LLAASEALARQVTQENFDKGLIYPPFSNIRKISAHIAANVAAKAYELGLATRLPQPENLV 575

Query: 64  KYAESCMYSPSYQNYR 80
           KYAESCMYSP Y++YR
Sbjct: 576 KYAESCMYSPVYRSYR 591

[Close]

BLAST of Spg025013 vs. ExPASy Swiss-Prot
Match: P36444 (NADP-dependent malic enzyme, chloroplastic OS=Flaveria pringlei OX=4226 GN=MODA PE=2 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 1.0e-29
Identity = 63/76 (82.89%), Postives = 69/76 (90.79%), Query Frame = 0


Query: 4   LLRAAEALAAQVTQDDFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLV 63
           LL A+EALA QVTQ+ FD GLIYPPFTNIRKISAHIAA VAAKAYELGLA+RLPQP++LV
Sbjct: 572 LLAASEALAEQVTQEHFDNGLIYPPFTNIRKISAHIAAKVAAKAYELGLASRLPQPENLV 631

Query: 64  KYAESCMYSPSYQNYR 80
            YAESCMYSP Y+NYR
Sbjct: 632 AYAESCMYSPKYRNYR 647

[Close]

BLAST of Spg025013 vs. ExPASy Swiss-Prot
Match: P12628 (NADP-dependent malic enzyme OS=Phaseolus vulgaris OX=3885 GN=ME1 PE=2 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 5.7e-28
Identity = 59/76 (77.63%), Postives = 71/76 (93.42%), Query Frame = 0


Query: 4   LLRAAEALAAQVTQDDFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLV 63
           LL A+EALAAQV+++++DKGLIYPPFTNIRKISA+IAA VAAKAY+LGLA+ L +PKDLV
Sbjct: 514 LLAASEALAAQVSEENYDKGLIYPPFTNIRKISANIAAKVAAKAYDLGLASHLKRPKDLV 573

Query: 64  KYAESCMYSPSYQNYR 80
           KYAESCMYSP Y++YR
Sbjct: 574 KYAESCMYSPGYRSYR 589

[Close]

BLAST of Spg025013 vs. ExPASy Swiss-Prot
Match: P22178 (NADP-dependent malic enzyme, chloroplastic OS=Flaveria trinervia OX=4227 GN=MOD1 PE=2 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 2.2e-27
Identity = 60/76 (78.95%), Postives = 68/76 (89.47%), Query Frame = 0


Query: 4   LLRAAEALAAQVTQDDFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLV 63
           LL A+EA A QVTQ+ FDKGLI+PPFT+IRKISAHIAA VAAKAYELGLA+RLPQP++LV
Sbjct: 573 LLAASEAPAEQVTQEHFDKGLIFPPFTSIRKISAHIAAKVAAKAYELGLASRLPQPENLV 632

Query: 64  KYAESCMYSPSYQNYR 80
            YAESCMYSP Y+ YR
Sbjct: 633 AYAESCMYSPKYRIYR 648

[Close]

BLAST of Spg025013 vs. ExPASy Swiss-Prot
Match: P16243 (NADP-dependent malic enzyme, chloroplastic OS=Zea mays OX=4577 GN=MOD1 PE=1 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 2.2e-27
Identity = 58/76 (76.32%), Postives = 68/76 (89.47%), Query Frame = 0


Query: 4   LLRAAEALAAQVTQDDFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLV 63
           LL A++ALA Q TQD+F+KG I+PPFT+IRKISAHIAA+VA KAYELGLATRLP P DLV
Sbjct: 561 LLAASKALADQATQDNFEKGSIFPPFTSIRKISAHIAAAVAGKAYELGLATRLPPPSDLV 620

Query: 64  KYAESCMYSPSYQNYR 80
           KYAE+CMY+P Y+NYR
Sbjct: 621 KYAENCMYTPVYRNYR 636

[Close]

BLAST of Spg025013 vs. ExPASy TrEMBL
Match: A0A6J1DJF8 (Malic enzyme OS=Momordica charantia OX=3673 GN=LOC111021036 PE=3 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 4.0e-32
Identity = 72/76 (94.74%), Postives = 74/76 (97.37%), Query Frame = 0


Query: 4   LLRAAEALAAQVTQDDFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLV 63
           LL A+EALA+QVTQDDFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDL 
Sbjct: 571 LLAASEALASQVTQDDFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLE 630

Query: 64  KYAESCMYSPSYQNYR 80
           KYAESCMYSPSYQNYR
Sbjct: 631 KYAESCMYSPSYQNYR 646

[Close]

BLAST of Spg025013 vs. ExPASy TrEMBL
Match: A0A1S4DWR5 (Malic enzyme OS=Cucumis melo OX=3656 GN=LOC103489969 PE=3 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 6.8e-32
Identity = 71/76 (93.42%), Postives = 75/76 (98.68%), Query Frame = 0


Query: 4   LLRAAEALAAQVTQDDFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLV 63
           LL A+EALA+QV+Q+DFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLV
Sbjct: 572 LLAASEALASQVSQEDFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLV 631

Query: 64  KYAESCMYSPSYQNYR 80
           KYAESCMYSPSYQNYR
Sbjct: 632 KYAESCMYSPSYQNYR 647

[Close]

BLAST of Spg025013 vs. ExPASy TrEMBL
Match: A0A5D3CAD9 (Malic enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold323G00330 PE=3 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 6.8e-32
Identity = 71/76 (93.42%), Postives = 75/76 (98.68%), Query Frame = 0


Query: 4   LLRAAEALAAQVTQDDFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLV 63
           LL A+EALA+QV+Q+DFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLV
Sbjct: 560 LLAASEALASQVSQEDFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLV 619

Query: 64  KYAESCMYSPSYQNYR 80
           KYAESCMYSPSYQNYR
Sbjct: 620 KYAESCMYSPSYQNYR 635

[Close]

BLAST of Spg025013 vs. ExPASy TrEMBL
Match: A0A5A7U9H4 (NADP-dependent malic enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold404G001150 PE=3 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 6.8e-32
Identity = 71/76 (93.42%), Postives = 75/76 (98.68%), Query Frame = 0


Query: 4   LLRAAEALAAQVTQDDFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLV 63
           LL A+EALA+QV+Q+DFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLV
Sbjct: 598 LLAASEALASQVSQEDFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLV 657

Query: 64  KYAESCMYSPSYQNYR 80
           KYAESCMYSPSYQNYR
Sbjct: 658 KYAESCMYSPSYQNYR 673

[Close]

BLAST of Spg025013 vs. ExPASy TrEMBL
Match: A0A0A0LAT4 (Malic enzyme OS=Cucumis sativus OX=3659 GN=Csa_3G444620 PE=3 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 6.8e-32
Identity = 71/76 (93.42%), Postives = 75/76 (98.68%), Query Frame = 0


Query: 4   LLRAAEALAAQVTQDDFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLV 63
           LL A+EALA+QV+Q+DFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLV
Sbjct: 572 LLAASEALASQVSQEDFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLV 631

Query: 64  KYAESCMYSPSYQNYR 80
           KYAESCMYSPSYQNYR
Sbjct: 632 KYAESCMYSPSYQNYR 647

[Close]

BLAST of Spg025013 vs. TAIR 10
Match: AT5G25880.1 (NADP-malic enzyme 3 )

HSP 1 Score: 118.2 bits (295), Expect = 2.9e-27
Identity = 57/76 (75.00%), Postives = 67/76 (88.16%), Query Frame = 0


Query: 4   LLRAAEALAAQVTQDDFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLV 63
           LL A+EALA+QVT+++F  GLIYPPF NIRKISA+IAASV AK YELGLA+ LP+PKDLV
Sbjct: 513 LLAASEALASQVTEENFANGLIYPPFANIRKISANIAASVGAKTYELGLASNLPRPKDLV 572

Query: 64  KYAESCMYSPSYQNYR 80
           K AESCMYSP Y+N+R
Sbjct: 573 KMAESCMYSPVYRNFR 588

[Close]

BLAST of Spg025013 vs. TAIR 10
Match: AT1G79750.1 (NADP-malic enzyme 4 )

HSP 1 Score: 111.7 bits (278), Expect = 2.7e-25
Identity = 54/76 (71.05%), Postives = 66/76 (86.84%), Query Frame = 0


Query: 4   LLRAAEALAAQVTQDDFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLV 63
           LL A+EALA ++ ++ ++KG+IYPPF NIRKISA IAA VAAKAYELGLATRLPQPK+L 
Sbjct: 571 LLAASEALAEELMEEHYEKGMIYPPFRNIRKISARIAAKVAAKAYELGLATRLPQPKELE 630

Query: 64  KYAESCMYSPSYQNYR 80
           + AES MYSPSY++YR
Sbjct: 631 QCAESSMYSPSYRSYR 646

[Close]

BLAST of Spg025013 vs. TAIR 10
Match: AT5G11670.1 (NADP-malic enzyme 2 )

HSP 1 Score: 110.9 bits (276), Expect = 4.6e-25
Identity = 54/76 (71.05%), Postives = 66/76 (86.84%), Query Frame = 0


Query: 4   LLRAAEALAAQVTQDDFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLV 63
           LL A+EALAAQVT++ +  GLIYPPF+NIR+ISA+IAA VAAK Y+LGLA+ LP+ KDLV
Sbjct: 513 LLAASEALAAQVTEEHYANGLIYPPFSNIREISANIAACVAAKTYDLGLASNLPRAKDLV 572

Query: 64  KYAESCMYSPSYQNYR 80
           K+AES MYSP Y+NYR
Sbjct: 573 KFAESSMYSPVYRNYR 588

[Close]

BLAST of Spg025013 vs. TAIR 10
Match: AT2G19900.1 (NADP-malic enzyme 1 )

HSP 1 Score: 109.0 bits (271), Expect = 1.8e-24
Identity = 53/76 (69.74%), Postives = 67/76 (88.16%), Query Frame = 0


Query: 4   LLRAAEALAAQVTQDDFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLV 63
           LL AAEALA QV++++++KG+IYP F++IRKISA IAA+VA KAYELGLA RLP+PKD+V
Sbjct: 506 LLAAAEALAGQVSKENYEKGMIYPSFSSIRKISAQIAANVATKAYELGLAGRLPRPKDIV 565

Query: 64  KYAESCMYSPSYQNYR 80
           K AES MYSP+Y+ YR
Sbjct: 566 KCAESSMYSPTYRLYR 581

[Close]

BLAST of Spg025013 vs. TAIR 10
Match: AT2G13560.1 (NAD-dependent malic enzyme 1 )

HSP 1 Score: 48.5 bits (114), Expect = 2.8e-06
Identity = 32/84 (38.10%), Postives = 50/84 (59.52%), Query Frame = 0


Query: 3   GLLRAA-EALAAQVTQDDFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLA-------T 62
           G+L+AA E LAA +++++  +G+IYPP + IR I+  IAA+V  +A E  L         
Sbjct: 532 GMLQAASECLAAYMSEEEVLEGIIYPPISRIRDITKRIAAAVIKEAIEEDLVEGYREMDA 591

Query: 63  RLPQPKD---LVKYAESCMYSPSY 76
           R  Q  D   L++Y E+ M++P Y
Sbjct: 592 REIQKLDEEGLMEYVENNMWNPEY 615

The following BLAST results are available for this feature:

BLAST of Spg025013 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038900124.1	4.8e-32	94.74	NADP-dependent malic enzyme-like [Benincasa hispida]	[more]
XP_022153564.1	8.2e-32	94.74	NADP-dependent malic enzyme-like [Momordica charantia]	[more]
XP_008447551.1	1.4e-31	93.42	PREDICTED: NADP-dependent malic enzyme [Cucumis melo] >XP_016900422.1 PREDICTED:...	[more]
TYK08505.1	1.4e-31	93.42	NADP-dependent malic enzyme [Cucumis melo var. makuwa]	[more]
KAA0050846.1	1.4e-31	93.42	NADP-dependent malic enzyme [Cucumis melo var. makuwa]	[more]

BLAST of Spg025013 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P51615	1.6e-30	84.21	NADP-dependent malic enzyme OS=Vitis vinifera OX=29760 PE=2 SV=1	[more]
P36444	1.0e-29	82.89	NADP-dependent malic enzyme, chloroplastic OS=Flaveria pringlei OX=4226 GN=MODA ...	[more]
P12628	5.7e-28	77.63	NADP-dependent malic enzyme OS=Phaseolus vulgaris OX=3885 GN=ME1 PE=2 SV=1	[more]
P22178	2.2e-27	78.95	NADP-dependent malic enzyme, chloroplastic OS=Flaveria trinervia OX=4227 GN=MOD1...	[more]
P16243	2.2e-27	76.32	NADP-dependent malic enzyme, chloroplastic OS=Zea mays OX=4577 GN=MOD1 PE=1 SV=1	[more]

BLAST of Spg025013 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DJF8	4.0e-32	94.74	Malic enzyme OS=Momordica charantia OX=3673 GN=LOC111021036 PE=3 SV=1	[more]
A0A1S4DWR5	6.8e-32	93.42	Malic enzyme OS=Cucumis melo OX=3656 GN=LOC103489969 PE=3 SV=1	[more]
A0A5D3CAD9	6.8e-32	93.42	Malic enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold323G00330 P...	[more]
A0A5A7U9H4	6.8e-32	93.42	NADP-dependent malic enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf...	[more]
A0A0A0LAT4	6.8e-32	93.42	Malic enzyme OS=Cucumis sativus OX=3659 GN=Csa_3G444620 PE=3 SV=1	[more]

BLAST of Spg025013 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G25880.1	2.9e-27	75.00	NADP-malic enzyme 3	[more]
AT1G79750.1	2.7e-25	71.05	NADP-malic enzyme 4	[more]
AT5G11670.1	4.6e-25	71.05	NADP-malic enzyme 2	[more]
AT2G19900.1	1.8e-24	69.74	NADP-malic enzyme 1	[more]
AT2G13560.1	2.8e-06	38.10	NAD-dependent malic enzyme 1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012302	Malic enzyme, NAD-binding	PFAM	PF03949	Malic_M	coord: 4..48 e-value: 6.3E-8 score: 32.2
None	No IPR available	GENE3D	3.40.50.720		coord: 3..79 e-value: 1.2E-21 score: 79.4
None	No IPR available	PANTHER	PTHR23406:SF76	NADP-DEPENDENT MALIC ENZYME 4, CHLOROPLASTIC	coord: 4..79
None	No IPR available	PANTHER	PTHR23406	MALIC ENZYME-RELATED	coord: 4..79
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 5..78

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg025013.1	Spg025013.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006108	malate metabolic process
biological_process	GO:0006090	pyruvate metabolic process
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0004471	malate dehydrogenase (decarboxylating) (NAD+) activity
molecular_function	GO:0004473	malate dehydrogenase (decarboxylating) (NADP+) activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0051287	NAD binding