Spg024762 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg024762
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionchaperone protein dnaJ 49
Locationscaffold12: 9325486 .. 9326544 (-)
RNA-Seq ExpressionSpg024762
SyntenySpg024762
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGGGAATAAGGACGAAGCTTTAAGATGTATCCGAATAGCGGAAGAAGCAATTGCGTCGGGAAATAAAGAGCGAGCATTGCGATTTATTAAAATTGCTCGGCGCCTTAATCAGAGTTTGCAAGTTGATGAGCTTTTGACGGCGTGCGAGGAGATCGATTCGGGATTACCTTCATCTACCTCCAATGAGAAACGCGCGGGAAAAGTTGACAGTGTTTCTGGTTCTGTGAAGCATGTTGATGGTTTGAATGGGGAACGTAATTACACTCTAGAACACGTTCAATTGGTTAGGCAGATAAAAACAACCAAGGACTATTACAGAATTCTTGGTGTGGAGAAAACTAGTTCGGCTGAAGAGATTAAGAGGGCTTATAAGAAATTGTCCTTGAAAGTTCACCCGGATAAGAACAAGGCACCTGGTTCAGATGAAGCATTCAAGAAATTATCCAAGGCTTTCATGTGTTTGAGTGACGATTCTTCAAGAAGGCAATATGACCACACGGCTCTGGTTGACCAATATGAGTACAATCAACAACATAATGTAAGACATAGGAGAAGGAGAACGGGTCATGATTTGTTTGAAGAAAATTTTGATCCTGATGAGATATTCAGGGCGTTCTTTGGCCAGGGGAATATGTTTCAGACGAGTCGTGCTTATACTTATAGAACTGGAGGTGGGGGAAGCCAACACAGGGCAGAGTCTGATGGGGGAGGCCCCAACTTGTTGATTTTTCTTCTAATGTTACCATTCTTGTTAATTTTCTTGTTGGCTTATTTGCCCTTTCCAGACCCGGACTATTCTTTGCATAAAAATCTATCCTATACCATTCCTATGGCTACTGAGAAACTTGGAGTGGAGTTTTTTGTCAGATCATCAGATTTTGATGTAAGATATCCTCTTGGAAGCCCAGCTCGAGCTGAAATAGAGAATAGTGTGATAAGGGATTATAGAAATATGGTTTGGCGTTATTGCCATGTAGAACTCCAGAGACGCCAGTGGAATAGAAATCTCCCAACTCCTCATTGTGAGAAGCTGAATAATCTCGGGTCAGCATAG

mRNA sequence

ATGGATGGGAATAAGGACGAAGCTTTAAGATGTATCCGAATAGCGGAAGAAGCAATTGCGTCGGGAAATAAAGAGCGAGCATTGCGATTTATTAAAATTGCTCGGCGCCTTAATCAGAGTTTGCAAGTTGATGAGCTTTTGACGGCGTGCGAGGAGATCGATTCGGGATTACCTTCATCTACCTCCAATGAGAAACGCGCGGGAAAAGTTGACAGTGTTTCTGGTTCTGTGAAGCATGTTGATGGTTTGAATGGGGAACGTAATTACACTCTAGAACACGTTCAATTGGTTAGGCAGATAAAAACAACCAAGGACTATTACAGAATTCTTGGTGTGGAGAAAACTAGTTCGGCTGAAGAGATTAAGAGGGCTTATAAGAAATTGTCCTTGAAAGTTCACCCGGATAAGAACAAGGCACCTGGTTCAGATGAAGCATTCAAGAAATTATCCAAGGCTTTCATGTGTTTGAGTGACGATTCTTCAAGAAGGCAATATGACCACACGGCTCTGGTTGACCAATATGAGTACAATCAACAACATAATGTAAGACATAGGAGAAGGAGAACGGGTCATGATTTGTTTGAAGAAAATTTTGATCCTGATGAGATATTCAGGGCGTTCTTTGGCCAGGGGAATATGTTTCAGACGAGTCGTGCTTATACTTATAGAACTGGAGGTGGGGGAAGCCAACACAGGGCAGAGTCTGATGGGGGAGGCCCCAACTTGTTGATTTTTCTTCTAATGTTACCATTCTTGTTAATTTTCTTGTTGGCTTATTTGCCCTTTCCAGACCCGGACTATTCTTTGCATAAAAATCTATCCTATACCATTCCTATGGCTACTGAGAAACTTGGAGTGGAGTTTTTTGTCAGATCATCAGATTTTGATGTAAGATATCCTCTTGGAAGCCCAGCTCGAGCTGAAATAGAGAATAGTGTGATAAGGGATTATAGAAATATGGTTTGGCGTTATTGCCATGTAGAACTCCAGAGACGCCAGTGGAATAGAAATCTCCCAACTCCTCATTGTGAGAAGCTGAATAATCTCGGGTCAGCATAG

Coding sequence (CDS)

ATGGATGGGAATAAGGACGAAGCTTTAAGATGTATCCGAATAGCGGAAGAAGCAATTGCGTCGGGAAATAAAGAGCGAGCATTGCGATTTATTAAAATTGCTCGGCGCCTTAATCAGAGTTTGCAAGTTGATGAGCTTTTGACGGCGTGCGAGGAGATCGATTCGGGATTACCTTCATCTACCTCCAATGAGAAACGCGCGGGAAAAGTTGACAGTGTTTCTGGTTCTGTGAAGCATGTTGATGGTTTGAATGGGGAACGTAATTACACTCTAGAACACGTTCAATTGGTTAGGCAGATAAAAACAACCAAGGACTATTACAGAATTCTTGGTGTGGAGAAAACTAGTTCGGCTGAAGAGATTAAGAGGGCTTATAAGAAATTGTCCTTGAAAGTTCACCCGGATAAGAACAAGGCACCTGGTTCAGATGAAGCATTCAAGAAATTATCCAAGGCTTTCATGTGTTTGAGTGACGATTCTTCAAGAAGGCAATATGACCACACGGCTCTGGTTGACCAATATGAGTACAATCAACAACATAATGTAAGACATAGGAGAAGGAGAACGGGTCATGATTTGTTTGAAGAAAATTTTGATCCTGATGAGATATTCAGGGCGTTCTTTGGCCAGGGGAATATGTTTCAGACGAGTCGTGCTTATACTTATAGAACTGGAGGTGGGGGAAGCCAACACAGGGCAGAGTCTGATGGGGGAGGCCCCAACTTGTTGATTTTTCTTCTAATGTTACCATTCTTGTTAATTTTCTTGTTGGCTTATTTGCCCTTTCCAGACCCGGACTATTCTTTGCATAAAAATCTATCCTATACCATTCCTATGGCTACTGAGAAACTTGGAGTGGAGTTTTTTGTCAGATCATCAGATTTTGATGTAAGATATCCTCTTGGAAGCCCAGCTCGAGCTGAAATAGAGAATAGTGTGATAAGGGATTATAGAAATATGGTTTGGCGTTATTGCCATGTAGAACTCCAGAGACGCCAGTGGAATAGAAATCTCCCAACTCCTCATTGTGAGAAGCTGAATAATCTCGGGTCAGCATAG

Protein sequence

MDGNKDEALRCIRIAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIDSGLPSSTSNEKRAGKVDSVSGSVKHVDGLNGERNYTLEHVQLVRQIKTTKDYYRILGVEKTSSAEEIKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDSSRRQYDHTALVDQYEYNQQHNVRHRRRRTGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTGGGGSQHRAESDGGGPNLLIFLLMLPFLLIFLLAYLPFPDPDYSLHKNLSYTIPMATEKLGVEFFVRSSDFDVRYPLGSPARAEIENSVIRDYRNMVWRYCHVELQRRQWNRNLPTPHCEKLNNLGSA
Homology
BLAST of Spg024762 vs. NCBI nr
Match: XP_038874732.1 (chaperone protein dnaJ 49 [Benincasa hispida] >XP_038874733.1 chaperone protein dnaJ 49 [Benincasa hispida] >XP_038874734.1 chaperone protein dnaJ 49 [Benincasa hispida])

HSP 1 Score: 634.4 bits (1635), Expect = 5.8e-178
Identity = 314/352 (89.20%), Postives = 333/352 (94.60%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIDSGLPSS 60
           MDGNKDEALRCIRIAEEAIASGNKERALRFIKIARRLNQSLQVDELLT CEEI SG PSS
Sbjct: 1   MDGNKDEALRCIRIAEEAIASGNKERALRFIKIARRLNQSLQVDELLTVCEEIGSGSPSS 60

Query: 61  TSNEKRAGKVDSVSGSVKHVDGLNGERNYTLEHVQLVRQIKTTKDYYRILGVEKTSSAEE 120
           + +EKRAGKV+S SGSVKHVDGL+GERNY++EHVQL+RQIKTT+DYYRILGVEKTSSAEE
Sbjct: 61  SFDEKRAGKVESASGSVKHVDGLSGERNYSIEHVQLIRQIKTTEDYYRILGVEKTSSAEE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDSSRRQYDHTALVDQYEYNQQH 180
           IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDD+ RRQYDHT LV QYEYNQQH
Sbjct: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVAQYEYNQQH 180

Query: 181 NVRHRRRRTGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTGGGGSQHRAESDGGGP 240
           NVRHRRRRTGHDLFEENFDPDEIFRAFFGQGNMFQTSR+YTYRTGG  SQ R ES GGGP
Sbjct: 181 NVRHRRRRTGHDLFEENFDPDEIFRAFFGQGNMFQTSRSYTYRTGGARSQQRTESAGGGP 240

Query: 241 NLLIFLLMLPFLLIFLLAYLPFPDPDYSLHKNLSYTIPMATEKLGVEFFVRSSDFDVRYP 300
           +LLI LLMLPFLLI LLAY+PFP+PDY+LHKNLSY+IP+ TEK GVEFFV+SSDFDVRYP
Sbjct: 241 SLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYSIPVITEKHGVEFFVKSSDFDVRYP 300

Query: 301 LGSPARAEIENSVIRDYRNMVWRYCHVELQRRQWNRNLPTPHCEKLNNLGSA 353
           LGSP RAEIENSV+RDYRNMVWRYCHVELQRRQWN+NLPTPHCEKLNNLG A
Sbjct: 301 LGSPGRAEIENSVLRDYRNMVWRYCHVELQRRQWNKNLPTPHCEKLNNLGVA 352

BLAST of Spg024762 vs. NCBI nr
Match: XP_022138829.1 (chaperone protein dnaJ 49 [Momordica charantia] >XP_022138830.1 chaperone protein dnaJ 49 [Momordica charantia])

HSP 1 Score: 616.7 bits (1589), Expect = 1.2e-172
Identity = 305/348 (87.64%), Postives = 329/348 (94.54%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIDSGLPSS 60
           MDGNKDEALRCI IAE AIASGNK RALRFIKIARRLN+SLQVDELL ACEE+DSG P+S
Sbjct: 1   MDGNKDEALRCIHIAEAAIASGNKARALRFIKIARRLNRSLQVDELLKACEEVDSGSPTS 60

Query: 61  TSNEKRAGKVDSVSGSVKHVDGLNGERNYTLEHVQLVRQIKTTKDYYRILGVEKTSSAEE 120
           +S+EKRAGKV+SV GS KHV+GLNGERNYT EHV+L+RQI TTKDYYRILGVEKTSSAEE
Sbjct: 61  SSDEKRAGKVESVPGSAKHVEGLNGERNYTTEHVRLIRQINTTKDYYRILGVEKTSSAEE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDSSRRQYDHTALVDQYEYNQQH 180
           IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDD+SRRQYDHT LVDQYEYNQQH
Sbjct: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTDLVDQYEYNQQH 180

Query: 181 NVRHRRRRTGHDLFEENFDPDEIFRAFFGQGNMF-QTSRAYTYRTGGGGSQHRAESDGGG 240
           NVR RRRRTGHDLFEENFDP+EIFRAFFGQGNMF  +SRAYTYRTGGGGSQ RAES+GGG
Sbjct: 181 NVRQRRRRTGHDLFEENFDPEEIFRAFFGQGNMFHSSSRAYTYRTGGGGSQQRAESNGGG 240

Query: 241 PNLLIFLLMLPFLLIFLLAYLPFPDPDYSLHKNLSYTIPMATEKLGVEFFVRSSDFDVRY 300
           PNLLI LLMLPFLLIFLLAYLPFP+PDYSLH NLSYTIPMATEK GVEF+V+SSDFDV+Y
Sbjct: 241 PNLLILLLMLPFLLIFLLAYLPFPEPDYSLHTNLSYTIPMATEKHGVEFYVKSSDFDVKY 300

Query: 301 PLGSPARAEIENSVIRDYRNMVWRYCHVELQRRQWNRNLPTPHCEKLN 348
           P GS ARAEIEN+V+RDY++MVWRYCH+ELQRRQWN+NLPTPHCEK+N
Sbjct: 301 PPGSLARAEIENTVLRDYKSMVWRYCHIELQRRQWNKNLPTPHCEKMN 348

BLAST of Spg024762 vs. NCBI nr
Match: XP_023547565.1 (chaperone protein dnaJ 49 [Cucurbita pepo subsp. pepo] >XP_023547566.1 chaperone protein dnaJ 49 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 614.4 bits (1583), Expect = 6.2e-172
Identity = 307/352 (87.22%), Postives = 331/352 (94.03%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIDSGLPSS 60
           MDGNKDEALRCIRIAEEAIASG+KERAL+FIKIA RLN+SL+V+ELL+ACE+IDS  PSS
Sbjct: 1   MDGNKDEALRCIRIAEEAIASGSKERALKFIKIACRLNRSLEVNELLSACEDIDSKSPSS 60

Query: 61  TSNEKRAGKVDSVSGSVKHVDGLNGERNYTLEHVQLVRQIKTTKDYYRILGVEKTSSAEE 120
           +S+ KRAGKV SVSGS  HVDGLNGERNY++EHVQL+RQIKT KDYY+ILGVEKTSSAEE
Sbjct: 61  SSDGKRAGKVHSVSGSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILGVEKTSSAEE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDSSRRQYDHTALVDQYEYNQQH 180
           IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDD+SRRQYDHTALVDQYEYNQQH
Sbjct: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALVDQYEYNQQH 180

Query: 181 NVRHRRRRTGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTGGGGSQHRAESDGGGP 240
           NV  RRRRTG DLFEENFDPDEIFRAFFGQGNMFQTSRAYTY TGG  SQ R ESDGGGP
Sbjct: 181 NV--RRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGP 240

Query: 241 NLLIFLLMLPFLLIFLLAYLPFPDPDYSLHKNLSYTIPMATEKLGVEFFVRSSDFDVRYP 300
           NLLIFLLMLPFLLI LLAY+PFP+PDYSLHKNLSY+IPMATEK GVEFFV+SSDFD RYP
Sbjct: 241 NLLIFLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHGVEFFVKSSDFDERYP 300

Query: 301 LGSPARAEIENSVIRDYRNMVWRYCHVELQRRQWNRNLPTPHCEKLNNLGSA 353
           LGS AR+EIE+SVIRDY+NMVWRYCHVELQRR+WN+NLPTPHCEK+NNLG A
Sbjct: 301 LGSGARSEIESSVIRDYKNMVWRYCHVELQRRKWNKNLPTPHCEKMNNLGFA 350

BLAST of Spg024762 vs. NCBI nr
Match: XP_016902270.1 (PREDICTED: chaperone protein dnaJ 49-like [Cucumis melo] >XP_016902271.1 PREDICTED: chaperone protein dnaJ 49-like [Cucumis melo] >XP_016902272.1 PREDICTED: chaperone protein dnaJ 49-like [Cucumis melo])

HSP 1 Score: 613.2 bits (1580), Expect = 1.4e-171
Identity = 304/352 (86.36%), Postives = 327/352 (92.90%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIDSGLPSS 60
           MDGNKDEALRCIRIAEE+IASGNKERALRFIKIARRLNQSLQ+DELLTACEE+ SG    
Sbjct: 1   MDGNKDEALRCIRIAEESIASGNKERALRFIKIARRLNQSLQIDELLTACEEMGSG---- 60

Query: 61  TSNEKRAGKVDSVSGSVKHVDGLNGERNYTLEHVQLVRQIKTTKDYYRILGVEKTSSAEE 120
           +S EKRAGK +SVSGSVKHVDGLNGERNY++EHVQL+RQIKTT DYY ILGVEKTSSAEE
Sbjct: 61  SSAEKRAGKGESVSGSVKHVDGLNGERNYSMEHVQLIRQIKTTMDYYGILGVEKTSSAEE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDSSRRQYDHTALVDQYEYNQQH 180
           IKRAY+KLSLKVHPDKNKAPGS+EAFKKLSKAF CLSDD+ RR+YD TALVDQYEYNQQH
Sbjct: 121 IKRAYRKLSLKVHPDKNKAPGSEEAFKKLSKAFSCLSDDTLRRRYDQTALVDQYEYNQQH 180

Query: 181 NVRHRRRRTGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTGGGGSQHRAESDGGGP 240
           NVRHRRRR GHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTGG GSQ R ESDGGGP
Sbjct: 181 NVRHRRRRNGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTGGAGSQQRTESDGGGP 240

Query: 241 NLLIFLLMLPFLLIFLLAYLPFPDPDYSLHKNLSYTIPMATEKLGVEFFVRSSDFDVRYP 300
           + LI LLMLPFLLI LLAY+PFP+P+Y+LHKNLSY+IPMATEK GVEFFV+SSDF+VRYP
Sbjct: 241 SFLIILLMLPFLLICLLAYMPFPEPEYALHKNLSYSIPMATEKHGVEFFVKSSDFNVRYP 300

Query: 301 LGSPARAEIENSVIRDYRNMVWRYCHVELQRRQWNRNLPTPHCEKLNNLGSA 353
           LGSP R EIENSV+RDYRNMVWRYCH+ELQRRQWN+NLPTPHCEKLN L  A
Sbjct: 301 LGSPGRVEIENSVLRDYRNMVWRYCHIELQRRQWNKNLPTPHCEKLNTLAVA 348

BLAST of Spg024762 vs. NCBI nr
Match: KAG6575070.1 (Chaperone protein dnaJ 49, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 612.1 bits (1577), Expect = 3.1e-171
Identity = 307/352 (87.22%), Postives = 328/352 (93.18%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIDSGLPSS 60
           MDGNKDEALRCIRIAEEAIASGNKERAL+FIKIA RLN+SL VDELL+ACEEID   PSS
Sbjct: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLGVDELLSACEEIDLKSPSS 60

Query: 61  TSNEKRAGKVDSVSGSVKHVDGLNGERNYTLEHVQLVRQIKTTKDYYRILGVEKTSSAEE 120
           +S+ KRAGKV SVSGS  HVDGLNGERNY++EHVQL+RQIKT KDYY+ILGVE+TSSAEE
Sbjct: 61  SSDGKRAGKVHSVSGSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILGVERTSSAEE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDSSRRQYDHTALVDQYEYNQQH 180
           IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDD+SRRQYDHTALVDQYEYNQQH
Sbjct: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALVDQYEYNQQH 180

Query: 181 NVRHRRRRTGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTGGGGSQHRAESDGGGP 240
           NV  RRRRTG DLFEENFDPDEIFRAFFGQGNMFQTSRAYTY TGG  SQ R ESDGGGP
Sbjct: 181 NV--RRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGP 240

Query: 241 NLLIFLLMLPFLLIFLLAYLPFPDPDYSLHKNLSYTIPMATEKLGVEFFVRSSDFDVRYP 300
           NLLI LLMLPFLLI LLAY+PFP+PDYSLHKNLSY+IPMATEK GVEFFV+SSDFD RYP
Sbjct: 241 NLLILLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHGVEFFVKSSDFDERYP 300

Query: 301 LGSPARAEIENSVIRDYRNMVWRYCHVELQRRQWNRNLPTPHCEKLNNLGSA 353
           LGS AR+EIE+SVIRDY+NMVWRYCHVELQRR+WN+NLPTPHCEK+NNLG A
Sbjct: 301 LGSGARSEIESSVIRDYKNMVWRYCHVELQRRKWNKNLPTPHCEKMNNLGFA 350

BLAST of Spg024762 vs. ExPASy Swiss-Prot
Match: Q9FH28 (Chaperone protein dnaJ 49 OS=Arabidopsis thaliana OX=3702 GN=ATJ49 PE=2 SV=2)

HSP 1 Score: 347.8 bits (891), Expect = 1.4e-94
Identity = 186/354 (52.54%), Postives = 255/354 (72.03%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIDSGLPSS 60
           MDGNKD+A RC+RIAE+AI SG+KERAL+FI +A+RLN SL VDEL+ AC+ +DS   +S
Sbjct: 1   MDGNKDDASRCLRIAEDAIVSGDKERALKFINMAKRLNPSLSVDELVAACDNLDSVSRNS 60

Query: 61  TSNEKRAGKVDSVSGSVKHVDGLNGERNYTLEHVQLVRQIKTTKDYYRILGVEKTSSAEE 120
           + +E    K+ ++ G    ++   G+  YT E+V LVR I    DYY ILG+EK  S +E
Sbjct: 61  SVSE----KLKTMDGDDDKLE--TGKMKYTEENVDLVRNIIRNNDYYAILGLEKNCSVDE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDSSRRQYDHTALVDQYEYNQQH 180
           I++AY+KLSLKVHPDKNKAPGS+EAFKK+SKAF CLSD +SRRQ+D   +VD++++ Q+ 
Sbjct: 121 IRKAYRKLSLKVHPDKNKAPGSEEAFKKVSKAFTCLSDGNSRRQFDQVGIVDEFDHVQRR 180

Query: 181 NVRHRRR-RTGHDLFEENFDPDEIFRAFFG-QGNMFQTSRAYTYRTGGGGSQHRAES-DG 240
           N R RRR  T +D F++ FDP+EIFR  FG Q  +F+ S A  YRT    +Q R E  + 
Sbjct: 181 NRRPRRRYNTRNDFFDDEFDPEEIFRTVFGQQREVFRASHA--YRTRQPRNQFREEEINV 240

Query: 241 GGPNLLIFLLMLPFLLIFLLAYLPFPDPDYSLHKNLSYTIPMATEKLGVEFFVRS-SDFD 300
            GP+ L  + +LPF L+ LLAYLPF +PDYSLHKN SY IP  T+   + F+VRS S FD
Sbjct: 241 AGPSCLTIIQILPFFLLLLLAYLPFSEPDYSLHKNQSYQIPKTTQNTEISFYVRSASAFD 300

Query: 301 VRYPLGSPARAEIENSVIRDYRNMVWRYCHVELQRRQWNRNLPTPHCEKLNNLG 351
            ++PL S ARA +E +VI++Y++ +++ C +ELQ+R+WN+ +PTPHC +L + G
Sbjct: 301 EKFPLSSSARANLEGNVIKEYKHFLFQSCRIELQKRRWNKKIPTPHCIELQDRG 346

BLAST of Spg024762 vs. ExPASy Swiss-Prot
Match: Q7ZXQ8 (DnaJ homolog subfamily B member 14 OS=Xenopus laevis OX=8355 GN=dnajb14 PE=2 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 1.2e-40
Identity = 119/350 (34.00%), Postives = 177/350 (50.57%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIDSGLPSS 60
           M+ N+DEA RC+RI + AI +G+KE+A RF   A RL  S +   LL A E+ D+    +
Sbjct: 1   MESNRDEAERCVRIGKAAIEAGDKEKARRFFSKAERLYPSSEARVLLDALEKNDTA--GN 60

Query: 61  TSNEKRAGKVDSVSGSVKHVDGLNGERNYTLEHVQLVRQIKTTKDYYRILGVEKTSSAEE 120
               ++  K      + K   G  G + +T + V  V++IK  K YY +LGV   +  E+
Sbjct: 61  GPQSEKMSKSTEQPKAEKDSSGDTG-KGHTQDQVDGVQRIKKCKTYYEVLGVSPDAGEED 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDSSRRQYDHTALVDQYEYNQQH 180
           +K+AY+KL+LK HPDKN APG+ EAFKK+  A+  LS+   R+QYD T   D        
Sbjct: 121 LKKAYRKLALKFHPDKNHAPGATEAFKKIGNAYAVLSNPEKRKQYDLTGSED-------- 180

Query: 181 NVRHRRRRTGHDL---FEENFDPDEIFRAFFGQGNMFQTSRAYTYRTG------------ 240
           NV++  R  G D    FE +  P+++F  FFG G  F +   +T+  G            
Sbjct: 181 NVQNNHRNGGFDYHRGFEADITPEDLFNMFFGGG--FPSGSVHTFSNGRTRYSHHQHHHH 240

Query: 241 GGGSQHRAESDGGGPNLLIFLLMLPFLLIFLLAYLPFPDPDYSLHKNLSYTIPMATEKLG 300
            G  +    +DGG    +  + ++  +L+ LL+ L   +P YSL+     TI   TE L 
Sbjct: 241 SGHDREEERADGGFSMFIQLMPIIVLILVSLLSQLMVSNPPYSLYPRSGQTIKRVTENLQ 300

Query: 301 VEFFVRSSDFDVRY--PLGSPARAEIENSVIRDYRNMVWRYCHVELQRRQ 334
           + ++V S DF   Y   L       IE   + + RN  WR    E Q++Q
Sbjct: 301 ISYYV-SKDFKSEYNGMLLQKLEKNIEEDYVANVRNNCWR----ERQQKQ 332

BLAST of Spg024762 vs. ExPASy Swiss-Prot
Match: Q149L6 (DnaJ homolog subfamily B member 14 OS=Mus musculus OX=10090 GN=Dnajb14 PE=2 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 2.9e-39
Identity = 122/348 (35.06%), Postives = 182/348 (52.30%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIDSGLPSS 60
           M+GN+DEA +C++IA EA+++GN+++A RF++ A +L        LL    +  S   SS
Sbjct: 1   MEGNRDEAEKCVQIAREALSAGNRDKAQRFLQKAEKLYPLPAARALLEIIMKNGSTAGSS 60

Query: 61  TSNEKRAGKVDSVSGSVKHVDGLNGE----RNYTLEHVQLVRQIKTTKDYYRILGVEKTS 120
           T   K  G  D    S    DG +G     + YT + V+ V  I   K+YY +LGV K +
Sbjct: 61  THCRKPPGSSDQSKPSCGK-DGTSGAGEGGKVYTKDQVEGVLSINKCKNYYEVLGVTKDA 120

Query: 121 SAEEIKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDSSRRQYDHTALVDQYEY 180
             E++K+AY+KL+LK HPDKN APG+ +AFKK+  A+  LS+   R+QYD T   +Q   
Sbjct: 121 GDEDLKKAYRKLALKFHPDKNHAPGATDAFKKIGNAYAVLSNPEKRKQYDLTGSEEQ-AC 180

Query: 181 NQQHNVRHRRRRTGHDLFEENFDPDEIFRAFFGQG------NMFQTSR-AYTYRTGGGGS 240
           N Q+N R    R      E +  P+++F  FFG G      + F   R AY+++     S
Sbjct: 181 NHQNNGRFNFHRG----CEADITPEDLFNIFFGGGFPSGSVHSFSNGRAAYSHQHQHRHS 240

Query: 241 QHRAESDGGGPNLLIFLLMLP---FLLIFLLAYLPFPDPDYSLH--KNLSYTIPMATEKL 300
            H  E +       +F+ ++P    +L+ LL+ L   +P YSL+       TI M TE L
Sbjct: 241 GHEREEERADGGFSVFIQLMPIIVLILVSLLSQLMVSNPPYSLYPRSGSGQTIKMQTENL 300

Query: 301 GVEFFVRSSDFDVRYPLGSPARAEIENSVIRDYRNMVWRYCHVELQRR 333
           GV ++V S DF   Y        ++E SV  DY   +   C  E Q++
Sbjct: 301 GVVYYV-SKDFKSEYK--GTLLQKVEKSVEEDYVTNIRNNCWKERQQK 339

BLAST of Spg024762 vs. ExPASy Swiss-Prot
Match: Q0IIE8 (DnaJ homolog subfamily B member 14 OS=Bos taurus OX=9913 GN=DNAJB14 PE=2 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 1.4e-38
Identity = 121/349 (34.67%), Postives = 180/349 (51.58%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIDSGLPSS 60
           M+GN+DEA +C+ IA EA+ +GN+E+A RF++ A +L        LL    +  S   +S
Sbjct: 1   MEGNRDEAEKCVEIAREALNAGNREKAQRFLQKAEKLYPLPSARALLEIIMKNGSTAGNS 60

Query: 61  TSNEKRAGKVD-SVSGSVKHVDGLNGE--RNYTLEHVQLVRQIKTTKDYYRILGVEKTSS 120
               K +G  D S     K     +GE  + YT + V  V  I   K+YY +LGV K + 
Sbjct: 61  PHCRKPSGGGDQSKPNCTKDSSSGSGESGKGYTKDQVDGVLSINKCKNYYEVLGVTKDAG 120

Query: 121 AEEIKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDSSRRQYDHTALVDQYEYN 180
            E++K+AY+KL+LK HPDKN APG+ +AFKK+  A+  LS+   R+QYD T   +Q   N
Sbjct: 121 DEDLKKAYRKLALKFHPDKNHAPGATDAFKKIGNAYAVLSNPEKRKQYDLTGNEEQ-ACN 180

Query: 181 QQHNVRHRRRRTGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTGGGG--------- 240
           QQ+N R    R      E +  P+++F  FFG G  F +   +++  G  G         
Sbjct: 181 QQNNGRFNFHRG----CEADITPEDLFNIFFGGG--FPSGSVHSFSNGRAGYSNQHQHRH 240

Query: 241 SQHRAESDGGGPNLLIFLLMLP---FLLIFLLAYLPFPDPDYSLH--KNLSYTIPMATEK 300
           S H  E + G     +F+ ++P    +L+ LL+ L   +P YSL+       TI M TE 
Sbjct: 241 SGHEREEERGDGGFSVFIQLMPIIVLILVSLLSQLMVSNPPYSLYPRSGSGQTIKMQTEN 300

Query: 301 LGVEFFVRSSDFDVRYPLGSPARAEIENSVIRDYRNMVWRYCHVELQRR 333
           LGV ++V + DF   Y        ++E SV  DY   +   C  E Q++
Sbjct: 301 LGVIYYV-NKDFKNEYK--GMLLQKVEKSVEEDYVTNIRNNCWKERQQK 339

BLAST of Spg024762 vs. ExPASy Swiss-Prot
Match: Q28I38 (DnaJ homolog subfamily B member 14 OS=Xenopus tropicalis OX=8364 GN=dnajb14 PE=2 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 1.4e-38
Identity = 114/342 (33.33%), Postives = 169/342 (49.42%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIDS--GLP 60
           M+ N+DEA RC+RIA+ AI +G+KE+A RF+  A RL  S +   LL A E+ D+    P
Sbjct: 1   MESNRDEAERCVRIAKAAIEAGDKEKAKRFLSKAERLYPSSEARALLQAFEKNDTAGNGP 60

Query: 61  SSTSNEKRAGKVDSVSGSVKHVDGLNGERNYTLEHVQLVRQIKTTKDYYRILGVEKTSSA 120
            S    K   +  +   S        G + +T + +  V++IK  K YY +LGV   +  
Sbjct: 61  QSAKMAKGTEQPKAEKDSNASASSDTG-KGHTQDQLDGVQRIKKCKTYYEVLGVSTDAGE 120

Query: 121 EEIKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDSSRRQYDHTALVDQYEYNQ 180
           E++K+AY+KL+LK HPDKN APG+ EAFKK+  A+  LS+   R+QYD T   DQ + N 
Sbjct: 121 EDLKKAYRKLALKFHPDKNHAPGATEAFKKIGNAYAVLSNPEKRKQYDLTGSEDQMQNN- 180

Query: 181 QHNVRHRRRRTGHDL---FEENFDPDEIFRAFFGQGNMFQTSRAYTYRTG---------- 240
                   R  G D    FE +  P+++F  FFG G  F +   +T+  G          
Sbjct: 181 -------HRNGGFDYHRGFEADITPEDLFNMFFGGG--FPSGSVHTFSNGRARYSHHQHH 240

Query: 241 --GGGSQHRAESDGGGPNLLIFLLMLPFLLIFLLAYLPFPDPDYSLHKNLSYTIPMATEK 300
              G  +    +DGG    +  + ++  +L+ LL+     +P YSL+          TE 
Sbjct: 241 HHSGHDREDERADGGFSMFIQLMPIIVLILVSLLSQFMVSNPPYSLYPRSGQATKRVTEN 300

Query: 301 LGVEFFVRSSDFDVRYP--LGSPARAEIENSVIRDYRNMVWR 324
           L + ++V S DF   Y   L       IE   + + RN  WR
Sbjct: 301 LQIAYYV-SKDFQSEYSGILLQKLEKNIEEDYVANVRNNCWR 330

BLAST of Spg024762 vs. ExPASy TrEMBL
Match: A0A6J1CB72 (chaperone protein dnaJ 49 OS=Momordica charantia OX=3673 GN=LOC111009903 PE=4 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 6.0e-173
Identity = 305/348 (87.64%), Postives = 329/348 (94.54%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIDSGLPSS 60
           MDGNKDEALRCI IAE AIASGNK RALRFIKIARRLN+SLQVDELL ACEE+DSG P+S
Sbjct: 1   MDGNKDEALRCIHIAEAAIASGNKARALRFIKIARRLNRSLQVDELLKACEEVDSGSPTS 60

Query: 61  TSNEKRAGKVDSVSGSVKHVDGLNGERNYTLEHVQLVRQIKTTKDYYRILGVEKTSSAEE 120
           +S+EKRAGKV+SV GS KHV+GLNGERNYT EHV+L+RQI TTKDYYRILGVEKTSSAEE
Sbjct: 61  SSDEKRAGKVESVPGSAKHVEGLNGERNYTTEHVRLIRQINTTKDYYRILGVEKTSSAEE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDSSRRQYDHTALVDQYEYNQQH 180
           IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDD+SRRQYDHT LVDQYEYNQQH
Sbjct: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTDLVDQYEYNQQH 180

Query: 181 NVRHRRRRTGHDLFEENFDPDEIFRAFFGQGNMF-QTSRAYTYRTGGGGSQHRAESDGGG 240
           NVR RRRRTGHDLFEENFDP+EIFRAFFGQGNMF  +SRAYTYRTGGGGSQ RAES+GGG
Sbjct: 181 NVRQRRRRTGHDLFEENFDPEEIFRAFFGQGNMFHSSSRAYTYRTGGGGSQQRAESNGGG 240

Query: 241 PNLLIFLLMLPFLLIFLLAYLPFPDPDYSLHKNLSYTIPMATEKLGVEFFVRSSDFDVRY 300
           PNLLI LLMLPFLLIFLLAYLPFP+PDYSLH NLSYTIPMATEK GVEF+V+SSDFDV+Y
Sbjct: 241 PNLLILLLMLPFLLIFLLAYLPFPEPDYSLHTNLSYTIPMATEKHGVEFYVKSSDFDVKY 300

Query: 301 PLGSPARAEIENSVIRDYRNMVWRYCHVELQRRQWNRNLPTPHCEKLN 348
           P GS ARAEIEN+V+RDY++MVWRYCH+ELQRRQWN+NLPTPHCEK+N
Sbjct: 301 PPGSLARAEIENTVLRDYKSMVWRYCHIELQRRQWNKNLPTPHCEKMN 348

BLAST of Spg024762 vs. ExPASy TrEMBL
Match: A0A1S4E2R1 (chaperone protein dnaJ 49-like OS=Cucumis melo OX=3656 GN=LOC103497850 PE=4 SV=1)

HSP 1 Score: 613.2 bits (1580), Expect = 6.7e-172
Identity = 304/352 (86.36%), Postives = 327/352 (92.90%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIDSGLPSS 60
           MDGNKDEALRCIRIAEE+IASGNKERALRFIKIARRLNQSLQ+DELLTACEE+ SG    
Sbjct: 1   MDGNKDEALRCIRIAEESIASGNKERALRFIKIARRLNQSLQIDELLTACEEMGSG---- 60

Query: 61  TSNEKRAGKVDSVSGSVKHVDGLNGERNYTLEHVQLVRQIKTTKDYYRILGVEKTSSAEE 120
           +S EKRAGK +SVSGSVKHVDGLNGERNY++EHVQL+RQIKTT DYY ILGVEKTSSAEE
Sbjct: 61  SSAEKRAGKGESVSGSVKHVDGLNGERNYSMEHVQLIRQIKTTMDYYGILGVEKTSSAEE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDSSRRQYDHTALVDQYEYNQQH 180
           IKRAY+KLSLKVHPDKNKAPGS+EAFKKLSKAF CLSDD+ RR+YD TALVDQYEYNQQH
Sbjct: 121 IKRAYRKLSLKVHPDKNKAPGSEEAFKKLSKAFSCLSDDTLRRRYDQTALVDQYEYNQQH 180

Query: 181 NVRHRRRRTGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTGGGGSQHRAESDGGGP 240
           NVRHRRRR GHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTGG GSQ R ESDGGGP
Sbjct: 181 NVRHRRRRNGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTGGAGSQQRTESDGGGP 240

Query: 241 NLLIFLLMLPFLLIFLLAYLPFPDPDYSLHKNLSYTIPMATEKLGVEFFVRSSDFDVRYP 300
           + LI LLMLPFLLI LLAY+PFP+P+Y+LHKNLSY+IPMATEK GVEFFV+SSDF+VRYP
Sbjct: 241 SFLIILLMLPFLLICLLAYMPFPEPEYALHKNLSYSIPMATEKHGVEFFVKSSDFNVRYP 300

Query: 301 LGSPARAEIENSVIRDYRNMVWRYCHVELQRRQWNRNLPTPHCEKLNNLGSA 353
           LGSP R EIENSV+RDYRNMVWRYCH+ELQRRQWN+NLPTPHCEKLN L  A
Sbjct: 301 LGSPGRVEIENSVLRDYRNMVWRYCHIELQRRQWNKNLPTPHCEKLNTLAVA 348

BLAST of Spg024762 vs. ExPASy TrEMBL
Match: A0A6J1H4U8 (chaperone protein dnaJ 49 OS=Cucurbita moschata OX=3662 GN=LOC111460072 PE=4 SV=1)

HSP 1 Score: 611.7 bits (1576), Expect = 1.9e-171
Identity = 305/352 (86.65%), Postives = 330/352 (93.75%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIDSGLPSS 60
           MDGNKDEALRCIRIAEEAIASGNKERAL+FIKIA RLN+SL+V+ELL+ACE+IDS  PSS
Sbjct: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLEVNELLSACEDIDSKSPSS 60

Query: 61  TSNEKRAGKVDSVSGSVKHVDGLNGERNYTLEHVQLVRQIKTTKDYYRILGVEKTSSAEE 120
           +S+ KRAGKV SVSGS  HVDGLNGERNY++EHVQL+RQIKT KDYY+ILGVEKTSSAEE
Sbjct: 61  SSDGKRAGKVHSVSGSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILGVEKTSSAEE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDSSRRQYDHTALVDQYEYNQQH 180
           IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLS+D+SRRQYDHTALVDQYEYNQQH
Sbjct: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSNDTSRRQYDHTALVDQYEYNQQH 180

Query: 181 NVRHRRRRTGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTGGGGSQHRAESDGGGP 240
           NV  RRRRTG DLFEENFDPDEIFRAFFGQGNMFQTSRAYTY TGG  SQ R ESDGGGP
Sbjct: 181 NV--RRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGP 240

Query: 241 NLLIFLLMLPFLLIFLLAYLPFPDPDYSLHKNLSYTIPMATEKLGVEFFVRSSDFDVRYP 300
           NLLI LLMLPFLLI LLAY+PFP+PDYSLHKNLSY+IPMATEK GVEFFV+SSDFD RYP
Sbjct: 241 NLLILLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHGVEFFVKSSDFDERYP 300

Query: 301 LGSPARAEIENSVIRDYRNMVWRYCHVELQRRQWNRNLPTPHCEKLNNLGSA 353
           LGS AR+EIE+SVIRDY+NMVWRYCH+ELQRR+WN+NLPTPHCEK+NNLG A
Sbjct: 301 LGSGARSEIESSVIRDYKNMVWRYCHIELQRRKWNKNLPTPHCEKMNNLGFA 350

BLAST of Spg024762 vs. ExPASy TrEMBL
Match: A0A6J1KXF3 (chaperone protein dnaJ 49 OS=Cucurbita maxima OX=3661 GN=LOC111499080 PE=4 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 7.4e-171
Identity = 305/352 (86.65%), Postives = 328/352 (93.18%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIDSGLPSS 60
           MDGNKDEALRCIRIAEEAIASGNKERAL+FIKIA RLN+SL+VDELL+ACEEIDS  PSS
Sbjct: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLEVDELLSACEEIDSKSPSS 60

Query: 61  TSNEKRAGKVDSVSGSVKHVDGLNGERNYTLEHVQLVRQIKTTKDYYRILGVEKTSSAEE 120
           +S+ KR GKV SVSGS  HVDGLNGERNY++E+VQL+RQIKT KDYY+ILGVEKTSSAEE
Sbjct: 61  SSDGKRTGKVHSVSGSANHVDGLNGERNYSIENVQLIRQIKTAKDYYKILGVEKTSSAEE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDSSRRQYDHTALVDQYEYNQQH 180
           IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDD+SRRQYDHTALVDQYEYNQQH
Sbjct: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALVDQYEYNQQH 180

Query: 181 NVRHRRRRTGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTGGGGSQHRAESDGGGP 240
           NV  RRRRTG DLFEENFDPDEIFRAFFGQGNMFQTSRAYTY TGG  SQ R ESDGGGP
Sbjct: 181 NV--RRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGP 240

Query: 241 NLLIFLLMLPFLLIFLLAYLPFPDPDYSLHKNLSYTIPMATEKLGVEFFVRSSDFDVRYP 300
           NLL+ LLMLPFLLI LLAY+PFP+PDYSLHKNLSY IPMATEK GVEFFV+SSDFD RYP
Sbjct: 241 NLLVLLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYIIPMATEKHGVEFFVKSSDFDERYP 300

Query: 301 LGSPARAEIENSVIRDYRNMVWRYCHVELQRRQWNRNLPTPHCEKLNNLGSA 353
           LGS AR+EIE+SVIRDY++MVWRYCHVELQRR+WN+NLPTPHCEK+NNLG A
Sbjct: 301 LGSRARSEIESSVIRDYKSMVWRYCHVELQRRKWNKNLPTPHCEKMNNLGFA 350

BLAST of Spg024762 vs. ExPASy TrEMBL
Match: A0A0A0KG46 (J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G306340 PE=4 SV=1)

HSP 1 Score: 607.1 bits (1564), Expect = 4.8e-170
Identity = 302/352 (85.80%), Postives = 323/352 (91.76%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIDSGLPSS 60
           MDGNKDEALRCIRIAEE+IASGNKERALRFIKIARRLNQS+QVDELL ACEEI SG    
Sbjct: 1   MDGNKDEALRCIRIAEESIASGNKERALRFIKIARRLNQSVQVDELLAACEEIGSG---- 60

Query: 61  TSNEKRAGKVDSVSGSVKHVDGLNGERNYTLEHVQLVRQIKTTKDYYRILGVEKTSSAEE 120
           +S EKRAGK +SVSGSVKH DGLNGERNY++EHVQL+RQIKTTKDYY ILGVEKTSSAEE
Sbjct: 61  SSEEKRAGKGESVSGSVKHGDGLNGERNYSMEHVQLIRQIKTTKDYYGILGVEKTSSAEE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDSSRRQYDHTALVDQYEYNQQH 180
           IKRAY+KLSLKVHPDKNKAPGS+EAFKKLSKAF CLSDD+ RRQYDHT LVDQYEYNQQH
Sbjct: 121 IKRAYRKLSLKVHPDKNKAPGSEEAFKKLSKAFSCLSDDTLRRQYDHTPLVDQYEYNQQH 180

Query: 181 NVRHRRRRTGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTGGGGSQHRAESDGGGP 240
           NVR RRRR GHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTGG GSQ R ES GGGP
Sbjct: 181 NVRQRRRRNGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTGGAGSQQRTESYGGGP 240

Query: 241 NLLIFLLMLPFLLIFLLAYLPFPDPDYSLHKNLSYTIPMATEKLGVEFFVRSSDFDVRYP 300
           N LI LLMLPFLLI LLAY+PFP+P+Y+LHK+LSY+IPMATEK GVEFFV+SSDFD RYP
Sbjct: 241 NFLIILLMLPFLLICLLAYMPFPEPEYALHKSLSYSIPMATEKHGVEFFVKSSDFDERYP 300

Query: 301 LGSPARAEIENSVIRDYRNMVWRYCHVELQRRQWNRNLPTPHCEKLNNLGSA 353
           LGSP R E+ENSV+RDYRNMVWRYCH+ELQRRQWN+NLPTPHCEKLN L  A
Sbjct: 301 LGSPGRVELENSVLRDYRNMVWRYCHIELQRRQWNKNLPTPHCEKLNTLAVA 348

BLAST of Spg024762 vs. TAIR 10
Match: AT5G49060.1 (Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) )

HSP 1 Score: 347.8 bits (891), Expect = 1.0e-95
Identity = 186/354 (52.54%), Postives = 255/354 (72.03%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIDSGLPSS 60
           MDGNKD+A RC+RIAE+AI SG+KERAL+FI +A+RLN SL VDEL+ AC+ +DS   +S
Sbjct: 1   MDGNKDDASRCLRIAEDAIVSGDKERALKFINMAKRLNPSLSVDELVAACDNLDSVSRNS 60

Query: 61  TSNEKRAGKVDSVSGSVKHVDGLNGERNYTLEHVQLVRQIKTTKDYYRILGVEKTSSAEE 120
           + +E    K+ ++ G    ++   G+  YT E+V LVR I    DYY ILG+EK  S +E
Sbjct: 61  SVSE----KLKTMDGDDDKLE--TGKMKYTEENVDLVRNIIRNNDYYAILGLEKNCSVDE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDSSRRQYDHTALVDQYEYNQQH 180
           I++AY+KLSLKVHPDKNKAPGS+EAFKK+SKAF CLSD +SRRQ+D   +VD++++ Q+ 
Sbjct: 121 IRKAYRKLSLKVHPDKNKAPGSEEAFKKVSKAFTCLSDGNSRRQFDQVGIVDEFDHVQRR 180

Query: 181 NVRHRRR-RTGHDLFEENFDPDEIFRAFFG-QGNMFQTSRAYTYRTGGGGSQHRAES-DG 240
           N R RRR  T +D F++ FDP+EIFR  FG Q  +F+ S A  YRT    +Q R E  + 
Sbjct: 181 NRRPRRRYNTRNDFFDDEFDPEEIFRTVFGQQREVFRASHA--YRTRQPRNQFREEEINV 240

Query: 241 GGPNLLIFLLMLPFLLIFLLAYLPFPDPDYSLHKNLSYTIPMATEKLGVEFFVRS-SDFD 300
            GP+ L  + +LPF L+ LLAYLPF +PDYSLHKN SY IP  T+   + F+VRS S FD
Sbjct: 241 AGPSCLTIIQILPFFLLLLLAYLPFSEPDYSLHKNQSYQIPKTTQNTEISFYVRSASAFD 300

Query: 301 VRYPLGSPARAEIENSVIRDYRNMVWRYCHVELQRRQWNRNLPTPHCEKLNNLG 351
            ++PL S ARA +E +VI++Y++ +++ C +ELQ+R+WN+ +PTPHC +L + G
Sbjct: 301 EKFPLSSSARANLEGNVIKEYKHFLFQSCRIELQKRRWNKKIPTPHCIELQDRG 346

BLAST of Spg024762 vs. TAIR 10
Match: AT3G57340.1 (Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) )

HSP 1 Score: 239.6 bits (610), Expect = 3.9e-63
Identity = 140/368 (38.04%), Postives = 212/368 (57.61%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIDSGLPSS 60
           MDGNKD+AL+C++I + A+ +G++ RAL+F+  ARRL+ +L +D+L++      S  P S
Sbjct: 1   MDGNKDDALKCLKICKSAMEAGDRPRALKFLAKARRLDPNLPIDDLVSELNNNKSDEPGS 60

Query: 61  TSNEKRAGKVDSVSGSVK--------HVDGLNGERNYTLEHVQLVRQIKTTKDYYRILGV 120
             +   A   DS + S +             +   +YT E + +VR+IK+ KDYY ILG+
Sbjct: 61  AKSPGSAAAKDSSNSSDRPSLRQRGSSTTSSSSSMSYTEEQISIVRKIKSKKDYYEILGL 120

Query: 121 EKTSSAEEIKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDSSRRQYDHTALVD 180
           E   S +++++AY+KLSLKVHPDKN+APGS+EAFK +SKAF CLS+D +R++YD +   D
Sbjct: 121 ESNCSVDDVRKAYRKLSLKVHPDKNQAPGSEEAFKSVSKAFQCLSNDEARKKYDVSG-SD 180

Query: 181 QYEYNQQHNVRHRRRRTGHDLFEENFDPDEIFRAFFGQGNM----FQTSRAYTYRTGGGG 240
           +  Y  + + R      G+  +E+ FDP+EIFR+FFG G         + A       G 
Sbjct: 181 EPIYQPRRSARSNGFNGGY-YYEDEFDPNEIFRSFFGGGGFGGGGMPPATAQFRSFNFGA 240

Query: 241 SQHRAESDGGGP----NLLIFLLMLPFLLIFLLAYLPFPDPDYSLHKNLSYTIPMATEKL 300
           ++ R  ++   P    N  I L +LP + I LL ++P   P Y L     Y     T+K 
Sbjct: 241 TRQRTANNNQAPDAGFNARILLQLLPVVFILLLNFMPSSQPVYQLSATYPYQYKFTTQK- 300

Query: 301 GVEFFVRSSDFDVRYPLGSPARAEIENSVIRDYRNMVWRYCHVELQRRQWNRNLPTPHCE 353
           GV +FV+SS F+  YP  S  R  +E  V RDY +++ + C  E+QR+QW     TPHC+
Sbjct: 301 GVNYFVKSSKFEQDYPRDSNDRHTLEEQVERDYVSILSQNCRYEMQRKQWGFVRETPHCD 360

BLAST of Spg024762 vs. TAIR 10
Match: AT3G57340.2 (Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) )

HSP 1 Score: 239.6 bits (610), Expect = 3.9e-63
Identity = 140/368 (38.04%), Postives = 212/368 (57.61%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALRFIKIARRLNQSLQVDELLTACEEIDSGLPSS 60
           MDGNKD+AL+C++I + A+ +G++ RAL+F+  ARRL+ +L +D+L++      S  P S
Sbjct: 1   MDGNKDDALKCLKICKSAMEAGDRPRALKFLAKARRLDPNLPIDDLVSELNNNKSDEPGS 60

Query: 61  TSNEKRAGKVDSVSGSVK--------HVDGLNGERNYTLEHVQLVRQIKTTKDYYRILGV 120
             +   A   DS + S +             +   +YT E + +VR+IK+ KDYY ILG+
Sbjct: 61  AKSPGSAAAKDSSNSSDRPSLRQRGSSTTSSSSSMSYTEEQISIVRKIKSKKDYYEILGL 120

Query: 121 EKTSSAEEIKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDSSRRQYDHTALVD 180
           E   S +++++AY+KLSLKVHPDKN+APGS+EAFK +SKAF CLS+D +R++YD +   D
Sbjct: 121 ESNCSVDDVRKAYRKLSLKVHPDKNQAPGSEEAFKSVSKAFQCLSNDEARKKYDVSG-SD 180

Query: 181 QYEYNQQHNVRHRRRRTGHDLFEENFDPDEIFRAFFGQGNM----FQTSRAYTYRTGGGG 240
           +  Y  + + R      G+  +E+ FDP+EIFR+FFG G         + A       G 
Sbjct: 181 EPIYQPRRSARSNGFNGGY-YYEDEFDPNEIFRSFFGGGGFGGGGMPPATAQFRSFNFGA 240

Query: 241 SQHRAESDGGGP----NLLIFLLMLPFLLIFLLAYLPFPDPDYSLHKNLSYTIPMATEKL 300
           ++ R  ++   P    N  I L +LP + I LL ++P   P Y L     Y     T+K 
Sbjct: 241 TRQRTANNNQAPDAGFNARILLQLLPVVFILLLNFMPSSQPVYQLSATYPYQYKFTTQK- 300

Query: 301 GVEFFVRSSDFDVRYPLGSPARAEIENSVIRDYRNMVWRYCHVELQRRQWNRNLPTPHCE 353
           GV +FV+SS F+  YP  S  R  +E  V RDY +++ + C  E+QR+QW     TPHC+
Sbjct: 301 GVNYFVKSSKFEQDYPRDSNDRHTLEEQVERDYVSILSQNCRYEMQRKQWGFVRETPHCD 360

BLAST of Spg024762 vs. TAIR 10
Match: AT5G05750.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 194.1 bits (492), Expect = 1.9e-49
Identity = 113/283 (39.93%), Postives = 173/283 (61.13%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALRFIKIARRLNQSLQVDELLT---------ACE 60
           MDGNKD+AL+C++I ++AI +G++ RAL+F++ ARRL+ +L +D L++         A E
Sbjct: 1   MDGNKDDALKCLKIGKDAIEAGDRSRALKFLEKARRLDPNLPIDGLVSDLKKQSDEPAAE 60

Query: 61  EIDSGLPSSTSNEKRAGKVDSVSGSVKHVDGLNGERNYTLEHVQLVRQIKTTKDYYRILG 120
           E   G  ++ S++          GS     G +   + T E   +VR+IK+ KDYY ILG
Sbjct: 61  EDSPGSAANESSKPSDRPSLRQRGSSSSAAGSSSSSSSTEEQRTIVREIKSKKDYYEILG 120

Query: 121 VEKTSSAEEIKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDSSRRQYDHTALV 180
           ++   S E+++++Y+KLSLKVHPDKNKAPGS+EAFK +SKAF CLS++ +RR+YD +   
Sbjct: 121 LKSNCSVEDLRKSYRKLSLKVHPDKNKAPGSEEAFKSVSKAFQCLSNEDTRRKYDGSG-S 180

Query: 181 DQYEYNQQHNVRHRRRRTGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTGGGGSQH 240
           D+  Y  + + R      G   +++ FD DEIFR+FFG G M   +  +     GGG++ 
Sbjct: 181 DEPAYQPRRDARRNNGFNG--FYDDEFDADEIFRSFFGGGEMNPATTQFRSFNFGGGTRT 240

Query: 241 RAESDGGGPNLLIFLLMLPFLLIFLLAYLPFPDPDYSLHKNLS 275
             ++   G N  + L +LP + I LL +LP P P YSL   ++
Sbjct: 241 ANQASDTGFNPRVLLQILPVVFILLLNFLPSPQPIYSLSHRIT 280

BLAST of Spg024762 vs. TAIR 10
Match: AT2G22360.1 (DNAJ heat shock family protein )

HSP 1 Score: 70.9 bits (172), Expect = 2.4e-12
Identity = 32/67 (47.76%), Postives = 45/67 (67.16%), Query Frame = 0

Query: 100 IKTTKDYYRILGVEKTSSAEEIKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDD 159
           ++   DYY +LGV K ++  EIK AY+KL+   HPD NK PG++E FK++S A+  LSDD
Sbjct: 81  VRADADYYSVLGVSKNATKAEIKSAYRKLARNYHPDVNKDPGAEEKFKEISNAYEVLSDD 140

Query: 160 SSRRQYD 167
             +  YD
Sbjct: 141 EKKSLYD 147

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038874732.15.8e-17889.20chaperone protein dnaJ 49 [Benincasa hispida] >XP_038874733.1 chaperone protein ... [more]
XP_022138829.11.2e-17287.64chaperone protein dnaJ 49 [Momordica charantia] >XP_022138830.1 chaperone protei... [more]
XP_023547565.16.2e-17287.22chaperone protein dnaJ 49 [Cucurbita pepo subsp. pepo] >XP_023547566.1 chaperone... [more]
XP_016902270.11.4e-17186.36PREDICTED: chaperone protein dnaJ 49-like [Cucumis melo] >XP_016902271.1 PREDICT... [more]
KAG6575070.13.1e-17187.22Chaperone protein dnaJ 49, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q9FH281.4e-9452.54Chaperone protein dnaJ 49 OS=Arabidopsis thaliana OX=3702 GN=ATJ49 PE=2 SV=2[more]
Q7ZXQ81.2e-4034.00DnaJ homolog subfamily B member 14 OS=Xenopus laevis OX=8355 GN=dnajb14 PE=2 SV=... [more]
Q149L62.9e-3935.06DnaJ homolog subfamily B member 14 OS=Mus musculus OX=10090 GN=Dnajb14 PE=2 SV=1[more]
Q0IIE81.4e-3834.67DnaJ homolog subfamily B member 14 OS=Bos taurus OX=9913 GN=DNAJB14 PE=2 SV=1[more]
Q28I381.4e-3833.33DnaJ homolog subfamily B member 14 OS=Xenopus tropicalis OX=8364 GN=dnajb14 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1CB726.0e-17387.64chaperone protein dnaJ 49 OS=Momordica charantia OX=3673 GN=LOC111009903 PE=4 SV... [more]
A0A1S4E2R16.7e-17286.36chaperone protein dnaJ 49-like OS=Cucumis melo OX=3656 GN=LOC103497850 PE=4 SV=1[more]
A0A6J1H4U81.9e-17186.65chaperone protein dnaJ 49 OS=Cucurbita moschata OX=3662 GN=LOC111460072 PE=4 SV=... [more]
A0A6J1KXF37.4e-17186.65chaperone protein dnaJ 49 OS=Cucurbita maxima OX=3661 GN=LOC111499080 PE=4 SV=1[more]
A0A0A0KG464.8e-17085.80J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G306340 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G49060.11.0e-9552.54Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) [more]
AT3G57340.13.9e-6338.04Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) [more]
AT3G57340.23.9e-6338.04Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) [more]
AT5G05750.11.9e-4939.93DNAJ heat shock N-terminal domain-containing protein [more]
AT2G22360.12.4e-1247.76DNAJ heat shock family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001623DnaJ domainPRINTSPR00625JDOMAINcoord: 125..140
score: 51.05
coord: 107..125
score: 51.32
coord: 141..161
score: 31.84
IPR001623DnaJ domainSMARTSM00271dnaj_3coord: 104..161
e-value: 9.4E-24
score: 94.9
IPR001623DnaJ domainPFAMPF00226DnaJcoord: 105..166
e-value: 2.3E-23
score: 82.1
IPR001623DnaJ domainPROSITEPS50076DNAJ_2coord: 105..169
score: 19.414032
IPR001623DnaJ domainCDDcd06257DnaJcoord: 105..158
e-value: 2.76288E-20
score: 81.0525
IPR015399Domain of unknown function DUF1977, DnaJ-likePFAMPF09320DUF1977coord: 263..333
e-value: 4.9E-8
score: 33.2
IPR036869Chaperone J-domain superfamilyGENE3D1.10.287.110DnaJ domaincoord: 102..220
e-value: 9.8E-29
score: 102.1
IPR036869Chaperone J-domain superfamilySUPERFAMILY46565Chaperone J-domaincoord: 101..213
NoneNo IPR availablePANTHERPTHR43908AT29763P-RELATEDcoord: 1..350
NoneNo IPR availablePANTHERPTHR43908:SF5CHAPERONE PROTEIN DNAJ 49coord: 1..350

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg024762.1Spg024762.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071218 cellular response to misfolded protein
biological_process GO:0051085 chaperone cofactor-dependent protein refolding
biological_process GO:0030433 ubiquitin-dependent ERAD pathway
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0030544 Hsp70 protein binding