Spg023987 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg023987
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionNADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-B
Locationscaffold13: 4937950 .. 4938462 (+)
RNA-Seq ExpressionSpg023987
SyntenySpg023987
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTGTTTCGTTCTCGACCTTTTGTATCTTCCTCTCTATGGTTGGTTAATAAGTTTAGATACTCTTTTGCTGCAAAAAATGAAGTTCACAATGGCATACAGCATATACAGGACATTGGCCTAAATATAAAGTATATTTTGTACTTACCGTTGGTATCTCTTGTAGGGCACTGAAAGAAGAAAAATATGCAGCCCGTCGTGCAATATTGCCTGTGCTTCAAGCTGAGGAAGATGAAAGGTAAAATGAATGATTTTCTATGTAGGCTGCTCTCAATCTTCCACTAAACTGGTTGAAACCATTTGGATCCCTGGACGGTTTTAACAAAGCTTAACATCATTTTCTAACCATGTCTGTGATCACTTTCCAGATTCGTCAAAGAGTGGAAGAGTTACCTCGAATATGAGGCTGAAGTCATGAAGGATGTGCCTGGTTGGAAAGTTGGTGAAAGTGTCTACAACTCCGGAAAATGGATGCCTCCAGCAACTGGTGAGCTACGACCTGAAGTCTGGTGA

mRNA sequence

ATGCTGTTTCGTTCTCGACCTTTTGTATCTTCCTCTCTATGGGCACTGAAAGAAGAAAAATATGCAGCCCGTCGTGCAATATTGCCTGTGCTTCAAGCTGAGGAAGATGAAAGATTCGTCAAAGAGTGGAAGAGTTACCTCGAATATGAGGCTGAAGTCATGAAGGATGTGCCTGGTTGGAAAGTTGGTGAAAGTGTCTACAACTCCGGAAAATGGATGCCTCCAGCAACTGGTGAGCTACGACCTGAAGTCTGGTGA

Coding sequence (CDS)

ATGCTGTTTCGTTCTCGACCTTTTGTATCTTCCTCTCTATGGGCACTGAAAGAAGAAAAATATGCAGCCCGTCGTGCAATATTGCCTGTGCTTCAAGCTGAGGAAGATGAAAGATTCGTCAAAGAGTGGAAGAGTTACCTCGAATATGAGGCTGAAGTCATGAAGGATGTGCCTGGTTGGAAAGTTGGTGAAAGTGTCTACAACTCCGGAAAATGGATGCCTCCAGCAACTGGTGAGCTACGACCTGAAGTCTGGTGA

Protein sequence

MLFRSRPFVSSSLWALKEEKYAARRAILPVLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMPPATGELRPEVW
Homology
BLAST of Spg023987 vs. NCBI nr
Match: XP_008460702.1 (PREDICTED: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-B [Cucumis melo])

HSP 1 Score: 150.2 bits (378), Expect = 8.0e-33
Identity = 71/71 (100.00%), Postives = 71/71 (100.00%), Query Frame = 0

Query: 15  ALKEEKYAARRAILPVLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMP 74
           ALKEEKYAARRAILPVLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMP
Sbjct: 73  ALKEEKYAARRAILPVLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMP 132

Query: 75  PATGELRPEVW 86
           PATGELRPEVW
Sbjct: 133 PATGELRPEVW 143

BLAST of Spg023987 vs. NCBI nr
Match: KAA0031969.1 (NADH dehydrogenase (ubiquinone) 1 alpha subcomplex subunit 13-B [Cucumis melo var. makuwa] >TYK16789.1 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex subunit 13-B [Cucumis melo var. makuwa])

HSP 1 Score: 150.2 bits (378), Expect = 8.0e-33
Identity = 71/71 (100.00%), Postives = 71/71 (100.00%), Query Frame = 0

Query: 15  ALKEEKYAARRAILPVLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMP 74
           ALKEEKYAARRAILPVLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMP
Sbjct: 67  ALKEEKYAARRAILPVLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMP 126

Query: 75  PATGELRPEVW 86
           PATGELRPEVW
Sbjct: 127 PATGELRPEVW 137

BLAST of Spg023987 vs. NCBI nr
Match: XP_004147172.1 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-B [Cucumis sativus] >KGN61547.1 hypothetical protein Csa_006591 [Cucumis sativus])

HSP 1 Score: 150.2 bits (378), Expect = 8.0e-33
Identity = 71/71 (100.00%), Postives = 71/71 (100.00%), Query Frame = 0

Query: 15  ALKEEKYAARRAILPVLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMP 74
           ALKEEKYAARRAILPVLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMP
Sbjct: 73  ALKEEKYAARRAILPVLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMP 132

Query: 75  PATGELRPEVW 86
           PATGELRPEVW
Sbjct: 133 PATGELRPEVW 143

BLAST of Spg023987 vs. NCBI nr
Match: XP_023006332.1 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-B-like [Cucurbita maxima])

HSP 1 Score: 149.1 bits (375), Expect = 1.8e-32
Identity = 70/71 (98.59%), Postives = 71/71 (100.00%), Query Frame = 0

Query: 15  ALKEEKYAARRAILPVLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMP 74
           ALKEEKYAARRAILP+LQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMP
Sbjct: 73  ALKEEKYAARRAILPMLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMP 132

Query: 75  PATGELRPEVW 86
           PATGELRPEVW
Sbjct: 133 PATGELRPEVW 143

BLAST of Spg023987 vs. NCBI nr
Match: XP_022959289.1 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-B [Cucurbita moschata] >XP_023549201.1 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-B [Cucurbita pepo subsp. pepo] >XP_038876329.1 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-B [Benincasa hispida] >KAG6574882.1 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-B, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013454.1 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 149.1 bits (375), Expect = 1.8e-32
Identity = 70/71 (98.59%), Postives = 71/71 (100.00%), Query Frame = 0

Query: 15  ALKEEKYAARRAILPVLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMP 74
           ALKEEKYAARRAILPVLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGE+VYNSGKWMP
Sbjct: 73  ALKEEKYAARRAILPVLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGENVYNSGKWMP 132

Query: 75  PATGELRPEVW 86
           PATGELRPEVW
Sbjct: 133 PATGELRPEVW 143

BLAST of Spg023987 vs. ExPASy Swiss-Prot
Match: O49313 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-B OS=Arabidopsis thaliana OX=3702 GN=At2g33220 PE=2 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 3.7e-33
Identity = 64/71 (90.14%), Postives = 69/71 (97.18%), Query Frame = 0

Query: 15  ALKEEKYAARRAILPVLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMP 74
           ALKEEKYAARRAILP+LQAEEDERFV EWK YLEYEA+VMKDVPGWKVGE+VYNSG+WMP
Sbjct: 73  ALKEEKYAARRAILPILQAEEDERFVSEWKKYLEYEADVMKDVPGWKVGENVYNSGRWMP 132

Query: 75  PATGELRPEVW 86
           PATGELRP+VW
Sbjct: 133 PATGELRPDVW 143

BLAST of Spg023987 vs. ExPASy Swiss-Prot
Match: Q8RWA7 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-A OS=Arabidopsis thaliana OX=3702 GN=MEE4 PE=1 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 1.1e-32
Identity = 63/71 (88.73%), Postives = 68/71 (95.77%), Query Frame = 0

Query: 15  ALKEEKYAARRAILPVLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMP 74
           ALKEEKYAARR ILP+LQAEEDERFV EWK YLEYEA+VMKDVPGWKVGE+VYNSG+WMP
Sbjct: 73  ALKEEKYAARRTILPILQAEEDERFVSEWKKYLEYEADVMKDVPGWKVGENVYNSGRWMP 132

Query: 75  PATGELRPEVW 86
           PATGELRP+VW
Sbjct: 133 PATGELRPDVW 143

BLAST of Spg023987 vs. ExPASy Swiss-Prot
Match: Q95KV7 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 OS=Bos taurus OX=9913 GN=NDUFA13 PE=1 SV=3)

HSP 1 Score: 73.9 bits (180), Expect = 9.5e-13
Identity = 35/65 (53.85%), Postives = 49/65 (75.38%), Query Frame = 0

Query: 16  LKEEKYAARRAILPVLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMPP 75
           L+ E + AR A++P+LQAE+D R ++  +  LE EA VMKDVPGWKVGESV+++ +W+ P
Sbjct: 60  LQIEDFEARIALMPLLQAEKDRRVLQMLRENLEEEATVMKDVPGWKVGESVFHTTRWVTP 119

Query: 76  ATGEL 81
             GEL
Sbjct: 120 MMGEL 124

BLAST of Spg023987 vs. ExPASy Swiss-Prot
Match: Q0MQ89 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 OS=Gorilla gorilla gorilla OX=9595 GN=NDUFA13 PE=2 SV=3)

HSP 1 Score: 72.8 bits (177), Expect = 2.1e-12
Identity = 35/65 (53.85%), Postives = 48/65 (73.85%), Query Frame = 0

Query: 16  LKEEKYAARRAILPVLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMPP 75
           L+ E + AR A+LP+LQAE D R ++  +  LE EA +MKDVP WKVGESV+++ +W+PP
Sbjct: 60  LQIEDFEARIALLPLLQAETDRRTLQMLRENLEEEAIIMKDVPDWKVGESVFHTTRWVPP 119

Query: 76  ATGEL 81
             GEL
Sbjct: 120 LIGEL 124

BLAST of Spg023987 vs. ExPASy Swiss-Prot
Match: Q9P0J0 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 OS=Homo sapiens OX=9606 GN=NDUFA13 PE=1 SV=3)

HSP 1 Score: 72.8 bits (177), Expect = 2.1e-12
Identity = 35/65 (53.85%), Postives = 48/65 (73.85%), Query Frame = 0

Query: 16  LKEEKYAARRAILPVLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMPP 75
           L+ E + AR A+LP+LQAE D R ++  +  LE EA +MKDVP WKVGESV+++ +W+PP
Sbjct: 60  LQIEDFEARIALLPLLQAETDRRTLQMLRENLEEEAIIMKDVPDWKVGESVFHTTRWVPP 119

Query: 76  ATGEL 81
             GEL
Sbjct: 120 LIGEL 124

BLAST of Spg023987 vs. ExPASy TrEMBL
Match: A0A5D3D2M4 (NADH dehydrogenase (Ubiquinone) 1 alpha subcomplex subunit 13-B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold96G00520 PE=3 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 3.9e-33
Identity = 71/71 (100.00%), Postives = 71/71 (100.00%), Query Frame = 0

Query: 15  ALKEEKYAARRAILPVLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMP 74
           ALKEEKYAARRAILPVLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMP
Sbjct: 67  ALKEEKYAARRAILPVLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMP 126

Query: 75  PATGELRPEVW 86
           PATGELRPEVW
Sbjct: 127 PATGELRPEVW 137

BLAST of Spg023987 vs. ExPASy TrEMBL
Match: A0A0A0LLE8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G169710 PE=3 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 3.9e-33
Identity = 71/71 (100.00%), Postives = 71/71 (100.00%), Query Frame = 0

Query: 15  ALKEEKYAARRAILPVLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMP 74
           ALKEEKYAARRAILPVLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMP
Sbjct: 73  ALKEEKYAARRAILPVLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMP 132

Query: 75  PATGELRPEVW 86
           PATGELRPEVW
Sbjct: 133 PATGELRPEVW 143

BLAST of Spg023987 vs. ExPASy TrEMBL
Match: A0A1S3CD20 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-B OS=Cucumis melo OX=3656 GN=LOC103499471 PE=3 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 3.9e-33
Identity = 71/71 (100.00%), Postives = 71/71 (100.00%), Query Frame = 0

Query: 15  ALKEEKYAARRAILPVLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMP 74
           ALKEEKYAARRAILPVLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMP
Sbjct: 73  ALKEEKYAARRAILPVLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMP 132

Query: 75  PATGELRPEVW 86
           PATGELRPEVW
Sbjct: 133 PATGELRPEVW 143

BLAST of Spg023987 vs. ExPASy TrEMBL
Match: A0A6J1KIP8 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-B OS=Cucurbita maxima OX=3661 GN=LOC111494802 PE=3 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 8.6e-33
Identity = 70/71 (98.59%), Postives = 71/71 (100.00%), Query Frame = 0

Query: 15  ALKEEKYAARRAILPVLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMP 74
           ALKEEKY+ARRAILPVLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMP
Sbjct: 73  ALKEEKYSARRAILPVLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMP 132

Query: 75  PATGELRPEVW 86
           PATGELRPEVW
Sbjct: 133 PATGELRPEVW 143

BLAST of Spg023987 vs. ExPASy TrEMBL
Match: A0A6J1KVK1 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-B-like OS=Cucurbita maxima OX=3661 GN=LOC111499103 PE=3 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 8.6e-33
Identity = 70/71 (98.59%), Postives = 71/71 (100.00%), Query Frame = 0

Query: 15  ALKEEKYAARRAILPVLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMP 74
           ALKEEKYAARRAILP+LQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMP
Sbjct: 73  ALKEEKYAARRAILPMLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMP 132

Query: 75  PATGELRPEVW 86
           PATGELRPEVW
Sbjct: 133 PATGELRPEVW 143

BLAST of Spg023987 vs. TAIR 10
Match: AT2G33220.1 (GRIM-19 protein )

HSP 1 Score: 141.7 bits (356), Expect = 2.6e-34
Identity = 64/71 (90.14%), Postives = 69/71 (97.18%), Query Frame = 0

Query: 15  ALKEEKYAARRAILPVLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMP 74
           ALKEEKYAARRAILP+LQAEEDERFV EWK YLEYEA+VMKDVPGWKVGE+VYNSG+WMP
Sbjct: 73  ALKEEKYAARRAILPILQAEEDERFVSEWKKYLEYEADVMKDVPGWKVGENVYNSGRWMP 132

Query: 75  PATGELRPEVW 86
           PATGELRP+VW
Sbjct: 133 PATGELRPDVW 143

BLAST of Spg023987 vs. TAIR 10
Match: AT1G04630.1 (GRIM-19 protein )

HSP 1 Score: 140.2 bits (352), Expect = 7.7e-34
Identity = 63/71 (88.73%), Postives = 68/71 (95.77%), Query Frame = 0

Query: 15  ALKEEKYAARRAILPVLQAEEDERFVKEWKSYLEYEAEVMKDVPGWKVGESVYNSGKWMP 74
           ALKEEKYAARR ILP+LQAEEDERFV EWK YLEYEA+VMKDVPGWKVGE+VYNSG+WMP
Sbjct: 73  ALKEEKYAARRTILPILQAEEDERFVSEWKKYLEYEADVMKDVPGWKVGENVYNSGRWMP 132

Query: 75  PATGELRPEVW 86
           PATGELRP+VW
Sbjct: 133 PATGELRPDVW 143

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008460702.18.0e-33100.00PREDICTED: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-B [Cucu... [more]
KAA0031969.18.0e-33100.00NADH dehydrogenase (ubiquinone) 1 alpha subcomplex subunit 13-B [Cucumis melo va... [more]
XP_004147172.18.0e-33100.00NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-B [Cucumis sativus... [more]
XP_023006332.11.8e-3298.59NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-B-like [Cucurbita ... [more]
XP_022959289.11.8e-3298.59NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-B [Cucurbita mosch... [more]
Match NameE-valueIdentityDescription
O493133.7e-3390.14NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-B OS=Arabidopsis t... [more]
Q8RWA71.1e-3288.73NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-A OS=Arabidopsis t... [more]
Q95KV79.5e-1353.85NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 OS=Bos taurus OX=9... [more]
Q0MQ892.1e-1253.85NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 OS=Gorilla gorilla... [more]
Q9P0J02.1e-1253.85NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 OS=Homo sapiens OX... [more]
Match NameE-valueIdentityDescription
A0A5D3D2M43.9e-33100.00NADH dehydrogenase (Ubiquinone) 1 alpha subcomplex subunit 13-B OS=Cucumis melo ... [more]
A0A0A0LLE83.9e-33100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G169710 PE=3 SV=1[more]
A0A1S3CD203.9e-33100.00NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-B OS=Cucumis melo ... [more]
A0A6J1KIP88.6e-3398.59NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-B OS=Cucurbita max... [more]
A0A6J1KVK18.6e-3398.59NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-B-like OS=Cucurbit... [more]
Match NameE-valueIdentityDescription
AT2G33220.12.6e-3490.14GRIM-19 protein [more]
AT1G04630.17.7e-3488.73GRIM-19 protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009346GRIM-19PFAMPF06212GRIM-19coord: 16..80
e-value: 2.3E-22
score: 79.4
IPR009346GRIM-19PANTHERPTHR12966NADH DEHYDROGENASE UBIQUINONE 1 ALPHA SUBCOMPLEX SUBUNIT 13coord: 15..85

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg023987.1Spg023987.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0022900 electron transport chain
cellular_component GO:0005747 mitochondrial respiratory chain complex I
molecular_function GO:0008137 NADH dehydrogenase (ubiquinone) activity