Spg023974 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg023974
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Pectinesterase
Location	scaffold13: 1526688 .. 1527779 (-)
RNA-Seq Expression	Spg023974
Synteny	Spg023974

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTTTCTTTAAAGCTATACTCCTTCTTTCTTTCTTGGAGCTCTCATGTTGCCTTGTTTTCAATTCAACCGTCTCAAAAGACGGAACTGGCAATTTTGCTCGTGTAAGCGATGCCATTGCAGCTGCACCAAACTTGAGTTCCAGCCGGTTTTACATCCATGTAAAAGCCGGCACCTACAACGAACATGTAGAGATACCGGAAGACAAAACTTTCATAAGTTTGATCGGAGACGGTGCATCCAACACGGTGATCGTCGACGGTCGAAACAATCACGACGGTTCTTCGACTACTGACTCAGCTACATTTAGTAAGCTAGCTAAGCCCTTTTTTTTTTATTTAAAAATCTGATTTAGAATATAAATGAAAGTATGTTCAAAGTTGTTTGCTAATTGATTGATAATTAATTTTGCAGCTGTGAAGGGTGAGCATTTTATTGCCCAATTCATCACATTTGAAAATTCGGCCGGACCCAAAAAGGGGCAAGCTGTCGCCTTTCTTGATGAAGCTAACCACACTGCTTATTACAACTGTGTGTTCAAGGGCTATCAAGACACTCTCTTCGTACGAGGAAGATTTCAATTTTATAAGAACTGCAGTGTATATGGAAGTGTCGACTTTATCTTCGGAGAAGGTCTCGTAGTCTTCCAAGACTGTACTATATATGCTAGATTGTGGGGGAGCACCATGACGGCACAATCCAAATCCCAAGTACAAGACTTAAGTGGTTTTACATTTCAGAACTGCAACATAACGACATCGTCGGAGATGGGGTCAGACAAAGTAACATTGTCTCTTGGACGACCGTGGAGAAGCTACTCTACCGTTATTGTCATGCAGTCATTTCTGGATGCCGTAGTTGAACCACAAGGGTGGTCCAAATGGTCCGGGATACCAATAAATCTACTATTTTATGCCGAATACAACAACTATGGTCCAGGAGCGAACACTTCCAAACGGGTGAACTGGCCAGGGTATCACATTTTGTACACTGATGAGGAAGCATCGAGGTTCACGGTGGAAAATTTCATTAATGGACTACAGTGGCTGCCCGAAACTCAAATTCCATTTAGAGGTGGATTGCATGGCTAA

mRNA sequence

ATGTCTTTCTTTAAAGCTATACTCCTTCTTTCTTTCTTGGAGCTCTCATGTTGCCTTGTTTTCAATTCAACCGTCTCAAAAGACGGAACTGGCAATTTTGCTCGTGTAAGCGATGCCATTGCAGCTGCACCAAACTTGAGTTCCAGCCGGTTTTACATCCATGTAAAAGCCGGCACCTACAACGAACATGTAGAGATACCGGAAGACAAAACTTTCATAAGTTTGATCGGAGACGGTGCATCCAACACGGTGATCGTCGACGGTCGAAACAATCACGACGGTTCTTCGACTACTGACTCAGCTACATTTACTGTGAAGGGTGAGCATTTTATTGCCCAATTCATCACATTTGAAAATTCGGCCGGACCCAAAAAGGGGCAAGCTGTCGCCTTTCTTGATGAAGCTAACCACACTGCTTATTACAACTGTGTGTTCAAGGGCTATCAAGACACTCTCTTCGTACGAGGAAGATTTCAATTTTATAAGAACTGCAGTGTATATGGAAGTGTCGACTTTATCTTCGGAGAAGGTCTCGTAGTCTTCCAAGACTGTACTATATATGCTAGATTGTGGGGGAGCACCATGACGGCACAATCCAAATCCCAAGTACAAGACTTAAGTGGTTTTACATTTCAGAACTGCAACATAACGACATCGTCGGAGATGGGGTCAGACAAAGTAACATTGTCTCTTGGACGACCGTGGAGAAGCTACTCTACCGTTATTGTCATGCAGTCATTTCTGGATGCCGTAGTTGAACCACAAGGGTGGTCCAAATGGTCCGGGATACCAATAAATCTACTATTTTATGCCGAATACAACAACTATGGTCCAGGAGCGAACACTTCCAAACGGGTGAACTGGCCAGGGTATCACATTTTGTACACTGATGAGGAAGCATCGAGGTTCACGGTGGAAAATTTCATTAATGGACTACAGTGGCTGCCCGAAACTCAAATTCCATTTAGAGGTGGATTGCATGGCTAA

Coding sequence (CDS)

ATGTCTTTCTTTAAAGCTATACTCCTTCTTTCTTTCTTGGAGCTCTCATGTTGCCTTGTTTTCAATTCAACCGTCTCAAAAGACGGAACTGGCAATTTTGCTCGTGTAAGCGATGCCATTGCAGCTGCACCAAACTTGAGTTCCAGCCGGTTTTACATCCATGTAAAAGCCGGCACCTACAACGAACATGTAGAGATACCGGAAGACAAAACTTTCATAAGTTTGATCGGAGACGGTGCATCCAACACGGTGATCGTCGACGGTCGAAACAATCACGACGGTTCTTCGACTACTGACTCAGCTACATTTACTGTGAAGGGTGAGCATTTTATTGCCCAATTCATCACATTTGAAAATTCGGCCGGACCCAAAAAGGGGCAAGCTGTCGCCTTTCTTGATGAAGCTAACCACACTGCTTATTACAACTGTGTGTTCAAGGGCTATCAAGACACTCTCTTCGTACGAGGAAGATTTCAATTTTATAAGAACTGCAGTGTATATGGAAGTGTCGACTTTATCTTCGGAGAAGGTCTCGTAGTCTTCCAAGACTGTACTATATATGCTAGATTGTGGGGGAGCACCATGACGGCACAATCCAAATCCCAAGTACAAGACTTAAGTGGTTTTACATTTCAGAACTGCAACATAACGACATCGTCGGAGATGGGGTCAGACAAAGTAACATTGTCTCTTGGACGACCGTGGAGAAGCTACTCTACCGTTATTGTCATGCAGTCATTTCTGGATGCCGTAGTTGAACCACAAGGGTGGTCCAAATGGTCCGGGATACCAATAAATCTACTATTTTATGCCGAATACAACAACTATGGTCCAGGAGCGAACACTTCCAAACGGGTGAACTGGCCAGGGTATCACATTTTGTACACTGATGAGGAAGCATCGAGGTTCACGGTGGAAAATTTCATTAATGGACTACAGTGGCTGCCCGAAACTCAAATTCCATTTAGAGGTGGATTGCATGGCTAA

Protein sequence

MSFFKAILLLSFLELSCCLVFNSTVSKDGTGNFARVSDAIAAAPNLSSSRFYIHVKAGTYNEHVEIPEDKTFISLIGDGASNTVIVDGRNNHDGSSTTDSATFTVKGEHFIAQFITFENSAGPKKGQAVAFLDEANHTAYYNCVFKGYQDTLFVRGRFQFYKNCSVYGSVDFIFGEGLVVFQDCTIYARLWGSTMTAQSKSQVQDLSGFTFQNCNITTSSEMGSDKVTLSLGRPWRSYSTVIVMQSFLDAVVEPQGWSKWSGIPINLLFYAEYNNYGPGANTSKRVNWPGYHILYTDEEASRFTVENFINGLQWLPETQIPFRGGLHG

Homology

[Close]

BLAST of Spg023974 vs. NCBI nr
Match: XP_022148123.1 (probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia])

HSP 1 Score: 463.0 bits (1190), Expect = 2.1e-126
Identity = 223/332 (67.17%), Postives = 268/332 (80.72%), Query Frame = 0


Query: 1   MSFFK--AILLLSFLELSCCLV-FNSTVSKDGTGNFARVSDAIAAAPNLSSSRFYIHVKA 60
           +S+F     LLL  +E S CL+ FN+TVS DG+G++  +SDAIAAAPN  S RFYIHVK 
Sbjct: 6   LSYFMWLVFLLLHSVEFSHCLITFNATVSLDGSGDYVHISDAIAAAPNNGSIRFYIHVKP 65

Query: 61  GTYNEHVEIPEDKTFISLIGDGASNTVIVDGRNNHDGSSTTDSATFTVKGEHFIAQFITF 120
           G Y EH+E+PE KTFISLIGD AS TVIVD R+NH G STT SATFTVK +HF+AQF+TF
Sbjct: 66  GIYKEHIEVPELKTFISLIGDDASTTVIVDDRSNHAGFSTTSSATFTVKAKHFVAQFLTF 125

Query: 121 ENSAGPKKGQAVAFLDEANHTAYYNCVFKGYQDTLFVRGRFQFYKNCSVYGSVDFIFGEG 180
           +NSAGP+ GQAVA L EAN+TAYY CVF GYQDTL+VRG FQ +K C VYGS+DFIFG+G
Sbjct: 126 QNSAGPENGQAVAVLSEANYTAYYKCVFMGYQDTLYVRGAFQLFKECDVYGSIDFIFGDG 185

Query: 181 LVVFQDCTIYARLWGSTMTAQSKSQVQDLSGFTFQNCNITTSSEMGS--DKVTLSLGRPW 240
           L +FQDC +YAR+ G+T+TAQSKS     SGFTFQNCN+T S E+ S  D+VT+SLGRPW
Sbjct: 186 LAIFQDCNVYARILGTTITAQSKSYKDQRSGFTFQNCNVTVSPEIQSIKDQVTVSLGRPW 245

Query: 241 RSYSTVIVMQSFLDAVVEPQGWSKWSGIPINLLFYAEYNNYGPGANTSKRVNWPGYHILY 300
           R+YSTVI M+SFLD +VEP GWS+W   P+ LLFYAEYNN G GA+TS+RV WPGYH+L+
Sbjct: 246 RNYSTVIFMESFLDDIVEPGGWSEWPRTPVYLLFYAEYNNSGSGADTSQRVKWPGYHVLH 305

Query: 301 TDEEASRFTVENFINGLQWLPETQIPFRGGLH 328
            ++EASRFTVE FI+GL+WL ET IPFR GL+
Sbjct: 306 NEDEASRFTVEIFIDGLEWLSETGIPFRRGLY 337

[Close]

BLAST of Spg023974 vs. NCBI nr
Match: XP_022150332.1 (probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia])

HSP 1 Score: 437.6 bits (1124), Expect = 9.7e-119
Identity = 210/333 (63.06%), Postives = 258/333 (77.48%), Query Frame = 0


Query: 1   MSFFKAILLL---SFLELSCCLVFNSTVSKDGTGNFARVSDAIAAAPNLSSSRFYIHVKA 60
           + FF   LL+    F++ S C +FN+TVS DG+GNF RVSDAIAAAPN S++RFYIHVK 
Sbjct: 6   LGFFVVSLLVVINHFVKFSYCFMFNATVSPDGSGNFVRVSDAIAAAPNFSTTRFYIHVKP 65

Query: 61  GTYNEHVEIPEDKTFISLIGDGASNTVIVDGRNNHDGSSTTDSATFTVKGEHFIAQFITF 120
           G Y EHVE+P  KTFI+LIG+ AS T+IVD R+N  G ST  +AT  VKG++F+AQF+TF
Sbjct: 66  GIYKEHVEVPVQKTFIALIGEDASTTIIVDNRSNGSGFSTQTTATLIVKGQNFMAQFLTF 125

Query: 121 ENSAGPKKGQAVAFLDEANHTAYYNCVFKGYQDTLFVRGRFQFYKNCSVYGSVDFIFGEG 180
           ENSA  K+GQA+A +D+A HTAYY CVF  YQDTL+     QF+K C +YG +DFIFG G
Sbjct: 126 ENSAEQKEGQAIAVMDQAKHTAYYKCVFLSYQDTLYAGAVPQFFKECDIYGRIDFIFGNG 185

Query: 181 LVVFQDCTIYARLWGS--TMTAQSKSQVQDLSGFTFQNCNITTSSEMGS--DKVTLSLGR 240
           LVVFQDC IYAR+ G+   +TAQSK+ V+D SGF FQNCN+T S E+ S  D VT+ LGR
Sbjct: 186 LVVFQDCNIYARIPGTHIIVTAQSKNAVEDQSGFIFQNCNVTVSPEIASSKDNVTMFLGR 245

Query: 241 PWRSYSTVIVMQSFLDAVVEPQGWSKWSGIPINLLFYAEYNNYGPGANTSKRVNWPGYHI 300
           PWR YS VI M+SFLD VV+P+GW +W G P+NLLFYAEYNN GPGA+TS+RV+WPGYH+
Sbjct: 246 PWRDYSMVIFMESFLDRVVQPKGWLEWPGRPVNLLFYAEYNNRGPGADTSQRVDWPGYHV 305

Query: 301 LYTDEEASRFTVENFINGLQWLPETQIPFRGGL 327
           L+   E ++FTVENFING +WLPET IPFR GL
Sbjct: 306 LHNAMEVTKFTVENFINGTKWLPETGIPFRAGL 338

[Close]

BLAST of Spg023974 vs. NCBI nr
Match: XP_023002625.1 (probable pectinesterase/pectinesterase inhibitor 39 [Cucurbita maxima])

HSP 1 Score: 436.8 bits (1122), Expect = 1.6e-118
Identity = 208/330 (63.03%), Postives = 256/330 (77.58%), Query Frame = 0


Query: 1   MSFFKAILLLSFLELSCCLVFNSTVSKDGTGNFARVSDAIAAAPNLSSSRFYIHVKAGTY 60
           M F  +ILLL FL+ S    FNSTVS DGTGNF ++SDAI AAPN +++RFYIH+K G Y
Sbjct: 7   MFFTLSILLLHFLKFSSTFDFNSTVSLDGTGNFVKISDAIVAAPNFTTTRFYIHIKPGIY 66

Query: 61  NEHVEIPEDKTFISLIGDGASNTVIVDGRNNHDGSSTTDSATFTVKGEHFIAQFITFENS 120
           NEH+EIP  KTFISLIGD AS T+IVD R+   G ST +SAT TV G +F+A+F+TF+NS
Sbjct: 67  NEHIEIPSQKTFISLIGDNASTTIIVDNRSKATGFSTANSATLTVNGANFMAKFLTFQNS 126

Query: 121 AGPKKGQAVAFLDEANHTAYYNCVFKGYQDTLFVRGRFQFYKNCSVYGSVDFIFGEGLVV 180
           AGPK GQAVA LD+A HTAYY CVF GYQDTL+     QF+K C +YG+VDFIFG GLVV
Sbjct: 127 AGPKMGQAVAVLDQAKHTAYYKCVFLGYQDTLYAAAVPQFFKECDIYGTVDFIFGNGLVV 186

Query: 181 FQDCTIYARLWG--STMTAQSKSQVQDLSGFTFQNCNITTSSEMGS--DKVTLSLGRPWR 240
           FQDC IYARL    ST+TAQSK+ + + SGF FQNC +T S E+ S  DKV + LGRPWR
Sbjct: 187 FQDCNIYARLVDIQSTVTAQSKANLDEQSGFVFQNCTVTVSPEIASSRDKVLVFLGRPWR 246

Query: 241 SYSTVIVMQSFLDAVVEPQGWSKWSGIPINLLFYAEYNNYGPGANTSKRVNWPGYHILYT 300
           +YS V  ++SFLD VV+P+GW +W G+P+NLL+Y EYNN G GANTS+RV+WPG+H+L++
Sbjct: 247 AYSMVAFVESFLDNVVQPKGWLEWLGVPVNLLYYGEYNNRGGGANTSQRVDWPGFHVLHS 306

Query: 301 DEEASRFTVENFINGLQWLPETQIPFRGGL 327
             + ++FTVENFING  WLP+T IPFR GL
Sbjct: 307 LTDVAKFTVENFINGTTWLPQTTIPFRSGL 336

[Close]

BLAST of Spg023974 vs. NCBI nr
Match: XP_023538248.1 (probable pectinesterase/pectinesterase inhibitor 39 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 434.9 bits (1117), Expect = 6.3e-118
Identity = 208/330 (63.03%), Postives = 255/330 (77.27%), Query Frame = 0


Query: 1   MSFFKAILLLSFLELSCCLVFNSTVSKDGTGNFARVSDAIAAAPNLSSSRFYIHVKAGTY 60
           M F  +ILLL FL+ S    FNSTVS DGTGNF ++SDAI AAPN +++RFYIH+K G Y
Sbjct: 7   MFFTLSILLLHFLKFSNTFDFNSTVSLDGTGNFVKISDAIVAAPNFTTTRFYIHIKPGIY 66

Query: 61  NEHVEIPEDKTFISLIGDGASNTVIVDGRNNHDGSSTTDSATFTVKGEHFIAQFITFENS 120
           NEH+E+P  KTFISLIGD AS T+IVD R+   G ST +SAT TV G +F+A+F+TF+NS
Sbjct: 67  NEHIEVPFQKTFISLIGDNASTTIIVDNRSKATGFSTANSATLTVNGANFMAKFLTFQNS 126

Query: 121 AGPKKGQAVAFLDEANHTAYYNCVFKGYQDTLFVRGRFQFYKNCSVYGSVDFIFGEGLVV 180
           AGPK GQAVA LD+A HTAYY CVF GYQDTL+     QF+K C +YG+VDFIFG GLVV
Sbjct: 127 AGPKMGQAVAVLDQAKHTAYYKCVFLGYQDTLYAAAVPQFFKECDIYGTVDFIFGNGLVV 186

Query: 181 FQDCTIYARLWG--STMTAQSKSQVQDLSGFTFQNCNITTSSEMGS--DKVTLSLGRPWR 240
           FQDC IYARL    +T+TAQSK+ + + SGF FQNC +T S E+ S  DKV + LGRPWR
Sbjct: 187 FQDCNIYARLVDIHTTVTAQSKANLDEQSGFVFQNCTVTVSPEIASSRDKVLVFLGRPWR 246

Query: 241 SYSTVIVMQSFLDAVVEPQGWSKWSGIPINLLFYAEYNNYGPGANTSKRVNWPGYHILYT 300
           +YS V  ++SFLD VV+P+GW +W G+P+NLL+Y EYNN G GANTS+RV+WPG+H+L++
Sbjct: 247 AYSMVAFVESFLDNVVQPKGWLEWLGVPVNLLYYGEYNNRGGGANTSQRVDWPGFHVLHS 306

Query: 301 DEEASRFTVENFINGLQWLPETQIPFRGGL 327
             E + FTVENFING  WLPET IPFR GL
Sbjct: 307 LTEVANFTVENFINGTTWLPETTIPFRAGL 336

[Close]

BLAST of Spg023974 vs. NCBI nr
Match: XP_022951717.1 (probable pectinesterase/pectinesterase inhibitor 39 [Cucurbita moschata])

HSP 1 Score: 433.7 bits (1114), Expect = 1.4e-117
Identity = 207/330 (62.73%), Postives = 256/330 (77.58%), Query Frame = 0


Query: 1   MSFFKAILLLSFLELSCCLVFNSTVSKDGTGNFARVSDAIAAAPNLSSSRFYIHVKAGTY 60
           M F  +ILLL FL+ S    FNSTVS DGTGNF ++SDAI AAPN +++RFYIH+K G Y
Sbjct: 7   MFFTLSILLLHFLKFSNTFDFNSTVSLDGTGNFVKISDAIVAAPNFTTTRFYIHIKPGIY 66

Query: 61  NEHVEIPEDKTFISLIGDGASNTVIVDGRNNHDGSSTTDSATFTVKGEHFIAQFITFENS 120
           NEH+E+P  KTFISLIGD AS T+IVD R+   G ST++SAT TV G +F+A+F+TF+NS
Sbjct: 67  NEHIEVPFQKTFISLIGDNASTTIIVDNRSKAAGFSTSNSATLTVNGANFMAKFLTFQNS 126

Query: 121 AGPKKGQAVAFLDEANHTAYYNCVFKGYQDTLFVRGRFQFYKNCSVYGSVDFIFGEGLVV 180
           AGPK GQAVA LD+A HTAYY CVF GYQDTL+     QF+K C +YG+VDFIFG GLVV
Sbjct: 127 AGPKMGQAVAVLDQAKHTAYYKCVFLGYQDTLYAAAVPQFFKECDIYGTVDFIFGNGLVV 186

Query: 181 FQDCTIYARLWG--STMTAQSKSQVQDLSGFTFQNCNITTSSEMGS--DKVTLSLGRPWR 240
           FQDC IYARL    +T+TAQSK+ + + SGF FQNC +T S E+ S  DKV + LGRPWR
Sbjct: 187 FQDCNIYARLVDIHTTVTAQSKANLDEQSGFVFQNCTVTVSPEIASSRDKVLVFLGRPWR 246

Query: 241 SYSTVIVMQSFLDAVVEPQGWSKWSGIPINLLFYAEYNNYGPGANTSKRVNWPGYHILYT 300
           +YS V  ++SFLD VV+P+GW +W G+P+NLL+Y EYNN G GANTS+RV+WPG+H+L++
Sbjct: 247 AYSMVAFVESFLDNVVQPKGWLEWLGVPVNLLYYGEYNNRGGGANTSQRVDWPGFHVLHS 306

Query: 301 DEEASRFTVENFINGLQWLPETQIPFRGGL 327
             + + FTVENFING  WLPET IPFR GL
Sbjct: 307 LTDVANFTVENFINGTTWLPETTIPFRAGL 336

[Close]

BLAST of Spg023974 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 290.0 bits (741), Expect = 3.3e-77
Identity = 141/313 (45.05%), Postives = 201/313 (64.22%), Query Frame = 0


Query: 24  TVSKDGTGNFARVSDAIAAAPNL---SSSRFYIHVKAGTYNEHVEIPEDKTFISLIGDGA 83
           TV ++GTGNF  ++ AIAAAPN    S+  F I+V AG Y E+VE+P++K ++ +IGDG 
Sbjct: 247 TVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGI 306

Query: 84  SNTVIVDGRNNHDGSSTTDSATFTVKGEHFIAQFITFENSAGPKKGQAVAFLDEANHTAY 143
           + TVI   R+  DG +T +SATF + G +FI   IT  N+AGP KGQAVA     + + +
Sbjct: 307 NQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVF 366

Query: 144 YNCVFKGYQDTLFVRGRFQFYKNCSVYGSVDFIFGEGLVVFQDCTIYARL----WGSTMT 203
           Y+C F+ YQDTL+     QFY+ C VYG+VDFIFG   VV Q+C +Y R       + +T
Sbjct: 367 YSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVT 426

Query: 204 AQSKSQVQDLSGFTFQNCNITTSSEMGSDKVTLS--LGRPWRSYSTVIVMQSFLDAVVEP 263
           AQ ++     +G     C I  + ++ +   T+   LGRPW+ YS  +VMQ+++D  +EP
Sbjct: 427 AQGRTDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEP 486

Query: 264 QGWSKWSG-IPINLLFYAEYNNYGPGANTSKRVNWPGYHILYTDEEASRFTVENFINGLQ 323
            GW+ WSG   ++ L+YAEYNN GPG++T+ RV WPGYH++    +AS FTV NF+ G  
Sbjct: 487 SGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVI-NATDASNFTVTNFLVGEG 546

Query: 324 WLPETQIPFRGGL 327
           W+ +T +PF GGL
Sbjct: 547 WIGQTGVPFVGGL 558

[Close]

BLAST of Spg023974 vs. ExPASy Swiss-Prot
Match: O81415 (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3702 GN=PME39 PE=2 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 3.3e-77
Identity = 144/312 (46.15%), Postives = 198/312 (63.46%), Query Frame = 0


Query: 21  FNSTVSKDGTGNFARVSDAIAAAPNLSSSRFYIHVKAGTYNEHVEIPEDKTFISLIGDGA 80
           +N +V+ DGTGNF  ++DA+ AAPN+S +RF I++K G Y E+VE+P+ KT I  IGDG 
Sbjct: 220 YNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGI 279

Query: 81  SNTVIVDGRNNHDGSSTTDSATFTVKGEHFIAQFITFENSAGPKKGQAVAFLDEANHTAY 140
             TVI   R+  DG ST  + T  VKG+ +IA+ I+F NSAGP K QAVAF   ++H+A+
Sbjct: 280 GKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSAF 339

Query: 141 YNCVFKGYQDTLFVRGRFQFYKNCSVYGSVDFIFGEGLVVFQDCTIYARLWGS----TMT 200
           Y C F GYQDTL+V    QFY+ C +YG++DFIFG   VVFQ+ ++YAR          T
Sbjct: 340 YRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAFT 399

Query: 201 AQSKSQVQDLSGFTFQNCNITTSSEMGSDKVTLS--LGRPWRSYSTVIVMQSFLDAVVEP 260
           AQS++Q    +G +  NC I  + ++   K      LGRPWR YS  ++++SF+D ++ P
Sbjct: 400 AQSRNQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIHP 459

Query: 261 QGWSKW-SGIPINLLFYAEYNNYGPGANTSKRVNWPGYHILYTDEEASRFTVENFINGLQ 320
            GW +      +  L+Y EY N GPGAN +KRV WPG+  +    EA++FTV  FI+G  
Sbjct: 460 AGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGST 519

Query: 321 WLPETQIPFRGG 326
           WL  T IPF  G
Sbjct: 520 WLNSTGIPFSLG 531

[Close]

BLAST of Spg023974 vs. ExPASy Swiss-Prot
Match: Q43062 (Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica OX=3760 PE=2 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 7.3e-77
Identity = 140/309 (45.31%), Postives = 198/309 (64.08%), Query Frame = 0


Query: 22  NSTVSKDGTGNFARVSDAIAAAPNLSSSRFYIHVKAGTYNEHVEIPEDKTFISLIGDGAS 81
           ++ V++DGTGNF  V+DA+ AAP+ S  R+ I++K GTY E+VEI + K  + +IGDG  
Sbjct: 208 DAIVAQDGTGNFTNVTDAVLAAPDYSMRRYVIYIKRGTYKENVEIKKKKWNLMMIGDGMD 267

Query: 82  NTVIVDGRNNHDGSSTTDSATFTVKGEHFIAQFITFENSAGPKKGQAVAFLDEANHTAYY 141
            T+I   R+  DG +T  SATF V G  FIA+ ITFEN+AGP+K QAVA   +++ + +Y
Sbjct: 268 ATIISGNRSFVDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFY 327

Query: 142 NCVFKGYQDTLFVRGRFQFYKNCSVYGSVDFIFGEGLVVFQDCTIYARL----WGSTMTA 201
            C  +GYQDTL+     QFY++C + G+VDFIFG+  VVFQ+C I A+       +++TA
Sbjct: 328 RCNIRGYQDTLYTHTMRQFYRDCKISGTVDFIFGDATVVFQNCQILAKKGLPNQKNSITA 387

Query: 202 QSKSQVQDLSGFTFQNCNITTSSEMGSDKVTLS---LGRPWRSYSTVIVMQSFLDAVVEP 261
           Q +    + +G + Q CNIT  S++ +  V  +   LGRPW+ YS  ++MQSFL  V+ P
Sbjct: 388 QGRKDPNEPTGISIQFCNITADSDLEAASVNSTPTYLGRPWKLYSRTVIMQSFLSNVIRP 447

Query: 262 QGWSKWSG-IPINLLFYAEYNNYGPGANTSKRVNWPGYHILYTDEEASRFTVENFINGLQ 321
           +GW +W+G   +N LFY EY NYGPGA    RV WPGY +     +A  +TV  FI G  
Sbjct: 448 EGWLEWNGDFALNSLFYGEYMNYGPGAGLGSRVKWPGYQVFNESTQAKNYTVAQFIEGNL 507

Query: 322 WLPETQIPF 323
           WLP T + +
Sbjct: 508 WLPSTGVKY 516

[Close]

BLAST of Spg023974 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 1.2e-76
Identity = 136/311 (43.73%), Postives = 198/311 (63.67%), Query Frame = 0


Query: 22  NSTVSKDGTGNFARVSDAIAAAPNLSSSRFYIHVKAGTYNEHVEIPEDKTFISLIGDGAS 81
           N  V++DG+GN   + +A+AAA     SR+ I++KAGTYNE++E+      I  +GDG  
Sbjct: 202 NIVVAQDGSGNVKTIQEAVAAASRAGGSRYVIYIKAGTYNENIEVKLKN--IMFVGDGIG 261

Query: 82  NTVIVDGRNNHDGSSTTDSATFTVKGEHFIAQFITFENSAGPKKGQAVAFLDEANHTAYY 141
            T+I   ++   G++T  SAT  V G++FIA+ IT  N+AGP   QAVA    ++ + +Y
Sbjct: 262 KTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAGPNNHQAVALRSGSDLSVFY 321

Query: 142 NCVFKGYQDTLFVRGRFQFYKNCSVYGSVDFIFGEGLVVFQDCTIYARL---WGSTMTAQ 201
            C F+GYQDTL+V  + QFY+ C +YG+VDFIFG   VV Q+C I+AR      +T+TAQ
Sbjct: 322 RCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIFARKPPNRTNTLTAQ 381

Query: 202 SKSQVQDLSGFTFQNCNITTSSEMG--SDKVTLSLGRPWRSYSTVIVMQSFLDAVVEPQG 261
            ++     +G    NC +T +S++      V   LGRPW+ YS  + +++FLD+++ P G
Sbjct: 382 GRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINPAG 441

Query: 262 WSKWSG-IPINLLFYAEYNNYGPGANTSKRVNWPGYHILYTDEEASRFTVENFINGLQWL 321
           W +WSG   +N L+YAEY N GPG++T+ RV W GYH+L +  + S+FTV NFI G  WL
Sbjct: 442 WMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNSWL 501

Query: 322 PETQIPFRGGL 327
           P T +PF  GL
Sbjct: 502 PATNVPFTSGL 510

[Close]

BLAST of Spg023974 vs. ExPASy Swiss-Prot
Match: O48711 (Probable pectinesterase/pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PME12 PE=2 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 4.7e-76
Identity = 143/309 (46.28%), Postives = 199/309 (64.40%), Query Frame = 0


Query: 25  VSKDGTGNFARVSDAIAAAPNLSSSRFYIHVKAGTYNEHVEIPEDKTFISLIGDGASNTV 84
           V+ DGTGNF+ +++AI+ APN+S+ R  I+VK G Y+E+++IP  KT I LIGDG+  T 
Sbjct: 240 VAADGTGNFSTINEAISFAPNMSNDRVLIYVKEGVYDENIDIPIYKTNIVLIGDGSDVTF 299

Query: 85  IVDGRNNHDGSSTTDSATFTVKGEHFIAQFITFENSAGPKKGQAVAFLDEANHTAYYNCV 144
           I   R+  DG +T  SAT  V GE F+A+ I   N+AGP+K QAVA    A+  A Y CV
Sbjct: 300 ITGNRSVGDGWTTFRSATLAVSGEGFLARDIMITNTAGPEKHQAVALRVNADFVALYRCV 359

Query: 145 FKGYQDTLFVRGRFQFYKNCSVYGSVDFIFGEGLVVFQDCTIYARL----WGSTMTAQSK 204
             GYQDTL+     QFY+ C +YG++D+IFG   VVFQ C I ++L      + +TAQS+
Sbjct: 360 IDGYQDTLYTHSFRQFYRECDIYGTIDYIFGNAAVVFQGCNIVSKLPMPGQFTVITAQSR 419

Query: 205 SQVQDLSGFTFQNCNITTSSEM--GSDKVTLSLGRPWRSYSTVIVMQSFLDAVVEPQGWS 264
               + +G + QNC+I  S ++   S+KV   LGRPWR +S  +VM+S++D  ++  GWS
Sbjct: 420 DTQDEDTGISMQNCSILASEDLFNSSNKVKSYLGRPWREFSRTVVMESYIDEFIDGSGWS 479

Query: 265 KWS-GIPINLLFYAEYNNYGPGANTSKRVNWPGYHILYTDEEASRFTVENFINGLQWLPE 324
           KW+ G  ++ L+Y EYNN GPG+ T KRVNWPG+HI+   E+A  FT   FI G  WL  
Sbjct: 480 KWNGGEALDTLYYGEYNNNGPGSETVKRVNWPGFHIM-GYEDAFNFTATEFITGDGWLGS 539

Query: 325 TQIPFRGGL 327
           T  P+  G+
Sbjct: 540 TSFPYDNGI 547

[Close]

BLAST of Spg023974 vs. ExPASy TrEMBL
Match: A0A6J1D381 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111016876 PE=4 SV=1)

HSP 1 Score: 463.0 bits (1190), Expect = 1.0e-126
Identity = 223/332 (67.17%), Postives = 268/332 (80.72%), Query Frame = 0


Query: 1   MSFFK--AILLLSFLELSCCLV-FNSTVSKDGTGNFARVSDAIAAAPNLSSSRFYIHVKA 60
           +S+F     LLL  +E S CL+ FN+TVS DG+G++  +SDAIAAAPN  S RFYIHVK 
Sbjct: 6   LSYFMWLVFLLLHSVEFSHCLITFNATVSLDGSGDYVHISDAIAAAPNNGSIRFYIHVKP 65

Query: 61  GTYNEHVEIPEDKTFISLIGDGASNTVIVDGRNNHDGSSTTDSATFTVKGEHFIAQFITF 120
           G Y EH+E+PE KTFISLIGD AS TVIVD R+NH G STT SATFTVK +HF+AQF+TF
Sbjct: 66  GIYKEHIEVPELKTFISLIGDDASTTVIVDDRSNHAGFSTTSSATFTVKAKHFVAQFLTF 125

Query: 121 ENSAGPKKGQAVAFLDEANHTAYYNCVFKGYQDTLFVRGRFQFYKNCSVYGSVDFIFGEG 180
           +NSAGP+ GQAVA L EAN+TAYY CVF GYQDTL+VRG FQ +K C VYGS+DFIFG+G
Sbjct: 126 QNSAGPENGQAVAVLSEANYTAYYKCVFMGYQDTLYVRGAFQLFKECDVYGSIDFIFGDG 185

Query: 181 LVVFQDCTIYARLWGSTMTAQSKSQVQDLSGFTFQNCNITTSSEMGS--DKVTLSLGRPW 240
           L +FQDC +YAR+ G+T+TAQSKS     SGFTFQNCN+T S E+ S  D+VT+SLGRPW
Sbjct: 186 LAIFQDCNVYARILGTTITAQSKSYKDQRSGFTFQNCNVTVSPEIQSIKDQVTVSLGRPW 245

Query: 241 RSYSTVIVMQSFLDAVVEPQGWSKWSGIPINLLFYAEYNNYGPGANTSKRVNWPGYHILY 300
           R+YSTVI M+SFLD +VEP GWS+W   P+ LLFYAEYNN G GA+TS+RV WPGYH+L+
Sbjct: 246 RNYSTVIFMESFLDDIVEPGGWSEWPRTPVYLLFYAEYNNSGSGADTSQRVKWPGYHVLH 305

Query: 301 TDEEASRFTVENFINGLQWLPETQIPFRGGLH 328
            ++EASRFTVE FI+GL+WL ET IPFR GL+
Sbjct: 306 NEDEASRFTVEIFIDGLEWLSETGIPFRRGLY 337

[Close]

BLAST of Spg023974 vs. ExPASy TrEMBL
Match: A0A6J1DAG5 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018528 PE=4 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 4.7e-119
Identity = 210/333 (63.06%), Postives = 258/333 (77.48%), Query Frame = 0


Query: 1   MSFFKAILLL---SFLELSCCLVFNSTVSKDGTGNFARVSDAIAAAPNLSSSRFYIHVKA 60
           + FF   LL+    F++ S C +FN+TVS DG+GNF RVSDAIAAAPN S++RFYIHVK 
Sbjct: 6   LGFFVVSLLVVINHFVKFSYCFMFNATVSPDGSGNFVRVSDAIAAAPNFSTTRFYIHVKP 65

Query: 61  GTYNEHVEIPEDKTFISLIGDGASNTVIVDGRNNHDGSSTTDSATFTVKGEHFIAQFITF 120
           G Y EHVE+P  KTFI+LIG+ AS T+IVD R+N  G ST  +AT  VKG++F+AQF+TF
Sbjct: 66  GIYKEHVEVPVQKTFIALIGEDASTTIIVDNRSNGSGFSTQTTATLIVKGQNFMAQFLTF 125

Query: 121 ENSAGPKKGQAVAFLDEANHTAYYNCVFKGYQDTLFVRGRFQFYKNCSVYGSVDFIFGEG 180
           ENSA  K+GQA+A +D+A HTAYY CVF  YQDTL+     QF+K C +YG +DFIFG G
Sbjct: 126 ENSAEQKEGQAIAVMDQAKHTAYYKCVFLSYQDTLYAGAVPQFFKECDIYGRIDFIFGNG 185

Query: 181 LVVFQDCTIYARLWGS--TMTAQSKSQVQDLSGFTFQNCNITTSSEMGS--DKVTLSLGR 240
           LVVFQDC IYAR+ G+   +TAQSK+ V+D SGF FQNCN+T S E+ S  D VT+ LGR
Sbjct: 186 LVVFQDCNIYARIPGTHIIVTAQSKNAVEDQSGFIFQNCNVTVSPEIASSKDNVTMFLGR 245

Query: 241 PWRSYSTVIVMQSFLDAVVEPQGWSKWSGIPINLLFYAEYNNYGPGANTSKRVNWPGYHI 300
           PWR YS VI M+SFLD VV+P+GW +W G P+NLLFYAEYNN GPGA+TS+RV+WPGYH+
Sbjct: 246 PWRDYSMVIFMESFLDRVVQPKGWLEWPGRPVNLLFYAEYNNRGPGADTSQRVDWPGYHV 305

Query: 301 LYTDEEASRFTVENFINGLQWLPETQIPFRGGL 327
           L+   E ++FTVENFING +WLPET IPFR GL
Sbjct: 306 LHNAMEVTKFTVENFINGTKWLPETGIPFRAGL 338

[Close]

BLAST of Spg023974 vs. ExPASy TrEMBL
Match: A0A6J1KPG6 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111496427 PE=4 SV=1)

HSP 1 Score: 436.8 bits (1122), Expect = 8.0e-119
Identity = 208/330 (63.03%), Postives = 256/330 (77.58%), Query Frame = 0


Query: 1   MSFFKAILLLSFLELSCCLVFNSTVSKDGTGNFARVSDAIAAAPNLSSSRFYIHVKAGTY 60
           M F  +ILLL FL+ S    FNSTVS DGTGNF ++SDAI AAPN +++RFYIH+K G Y
Sbjct: 7   MFFTLSILLLHFLKFSSTFDFNSTVSLDGTGNFVKISDAIVAAPNFTTTRFYIHIKPGIY 66

Query: 61  NEHVEIPEDKTFISLIGDGASNTVIVDGRNNHDGSSTTDSATFTVKGEHFIAQFITFENS 120
           NEH+EIP  KTFISLIGD AS T+IVD R+   G ST +SAT TV G +F+A+F+TF+NS
Sbjct: 67  NEHIEIPSQKTFISLIGDNASTTIIVDNRSKATGFSTANSATLTVNGANFMAKFLTFQNS 126

Query: 121 AGPKKGQAVAFLDEANHTAYYNCVFKGYQDTLFVRGRFQFYKNCSVYGSVDFIFGEGLVV 180
           AGPK GQAVA LD+A HTAYY CVF GYQDTL+     QF+K C +YG+VDFIFG GLVV
Sbjct: 127 AGPKMGQAVAVLDQAKHTAYYKCVFLGYQDTLYAAAVPQFFKECDIYGTVDFIFGNGLVV 186

Query: 181 FQDCTIYARLWG--STMTAQSKSQVQDLSGFTFQNCNITTSSEMGS--DKVTLSLGRPWR 240
           FQDC IYARL    ST+TAQSK+ + + SGF FQNC +T S E+ S  DKV + LGRPWR
Sbjct: 187 FQDCNIYARLVDIQSTVTAQSKANLDEQSGFVFQNCTVTVSPEIASSRDKVLVFLGRPWR 246

Query: 241 SYSTVIVMQSFLDAVVEPQGWSKWSGIPINLLFYAEYNNYGPGANTSKRVNWPGYHILYT 300
           +YS V  ++SFLD VV+P+GW +W G+P+NLL+Y EYNN G GANTS+RV+WPG+H+L++
Sbjct: 247 AYSMVAFVESFLDNVVQPKGWLEWLGVPVNLLYYGEYNNRGGGANTSQRVDWPGFHVLHS 306

Query: 301 DEEASRFTVENFINGLQWLPETQIPFRGGL 327
             + ++FTVENFING  WLP+T IPFR GL
Sbjct: 307 LTDVAKFTVENFINGTTWLPQTTIPFRSGL 336

[Close]

BLAST of Spg023974 vs. ExPASy TrEMBL
Match: A0A6J1GIG0 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111454459 PE=4 SV=1)

HSP 1 Score: 433.7 bits (1114), Expect = 6.8e-118
Identity = 207/330 (62.73%), Postives = 256/330 (77.58%), Query Frame = 0


Query: 1   MSFFKAILLLSFLELSCCLVFNSTVSKDGTGNFARVSDAIAAAPNLSSSRFYIHVKAGTY 60
           M F  +ILLL FL+ S    FNSTVS DGTGNF ++SDAI AAPN +++RFYIH+K G Y
Sbjct: 7   MFFTLSILLLHFLKFSNTFDFNSTVSLDGTGNFVKISDAIVAAPNFTTTRFYIHIKPGIY 66

Query: 61  NEHVEIPEDKTFISLIGDGASNTVIVDGRNNHDGSSTTDSATFTVKGEHFIAQFITFENS 120
           NEH+E+P  KTFISLIGD AS T+IVD R+   G ST++SAT TV G +F+A+F+TF+NS
Sbjct: 67  NEHIEVPFQKTFISLIGDNASTTIIVDNRSKAAGFSTSNSATLTVNGANFMAKFLTFQNS 126

Query: 121 AGPKKGQAVAFLDEANHTAYYNCVFKGYQDTLFVRGRFQFYKNCSVYGSVDFIFGEGLVV 180
           AGPK GQAVA LD+A HTAYY CVF GYQDTL+     QF+K C +YG+VDFIFG GLVV
Sbjct: 127 AGPKMGQAVAVLDQAKHTAYYKCVFLGYQDTLYAAAVPQFFKECDIYGTVDFIFGNGLVV 186

Query: 181 FQDCTIYARLWG--STMTAQSKSQVQDLSGFTFQNCNITTSSEMGS--DKVTLSLGRPWR 240
           FQDC IYARL    +T+TAQSK+ + + SGF FQNC +T S E+ S  DKV + LGRPWR
Sbjct: 187 FQDCNIYARLVDIHTTVTAQSKANLDEQSGFVFQNCTVTVSPEIASSRDKVLVFLGRPWR 246

Query: 241 SYSTVIVMQSFLDAVVEPQGWSKWSGIPINLLFYAEYNNYGPGANTSKRVNWPGYHILYT 300
           +YS V  ++SFLD VV+P+GW +W G+P+NLL+Y EYNN G GANTS+RV+WPG+H+L++
Sbjct: 247 AYSMVAFVESFLDNVVQPKGWLEWLGVPVNLLYYGEYNNRGGGANTSQRVDWPGFHVLHS 306

Query: 301 DEEASRFTVENFINGLQWLPETQIPFRGGL 327
             + + FTVENFING  WLPET IPFR GL
Sbjct: 307 LTDVANFTVENFINGTTWLPETTIPFRAGL 336

[Close]

BLAST of Spg023974 vs. ExPASy TrEMBL
Match: A0A0A0L1M3 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_4G260430 PE=4 SV=1)

HSP 1 Score: 424.9 bits (1091), Expect = 3.1e-115
Identity = 205/329 (62.31%), Postives = 250/329 (75.99%), Query Frame = 0


Query: 1   MSFFKAILLLSFLELSCCLVFNSTVSKDGTGNFARVSDAIAAAPNLSSSRFYIHVKAGTY 60
           M F   +L++  +E S C  FNSTVS DGT NF +++DAIAAAPN S++RFYIHVK GTY
Sbjct: 1   MIFVLYLLVVVIVEFSDCFSFNSTVSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTY 60

Query: 61  NEHVEIPEDKTFISLIGDGASNTVIVDGRNNHDGSSTTDSATFTVKGEHFIAQFITFENS 120
            E +E+P +KT I+LIGD +S T+IV+ R+N  GSST  SAT TV G +F+AQF+TF+N+
Sbjct: 61  QEIIEVPYEKTCIALIGDDSSTTIIVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNT 120

Query: 121 AGPKKGQAVAFLDEANHTAYYNCVFKGYQDTLFVRGRFQFYKNCSVYGSVDFIFGEGLVV 180
           AGP +GQA+A LD+A HTAYY CVF GYQDTL+     QF+K C +YGSVDFIFG GLVV
Sbjct: 121 AGPNEGQAIALLDQATHTAYYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVV 180

Query: 181 FQDCTIYARLWG--STMTAQSKSQVQDLSGFTFQNCNITTSSEMGS--DKVTLSLGRPWR 240
           FQDC IYARL+    T+TAQSK  +  LSGF FQNC +T S E+ S  DKVT+ LGRPWR
Sbjct: 181 FQDCNIYARLFDVQITVTAQSKPSLNSLSGFIFQNCKVTVSPEIASSKDKVTVFLGRPWR 240

Query: 241 SYSTVIVMQSFLDAVVEPQGWSKWSGIPINLLFYAEYNNYGPGANTSKRVNWPGYHILYT 300
            YS V+ + SFLD VV+P+GW +W G+P NLL+YAEYNN G GANTS+RVNWPGYH L  
Sbjct: 241 MYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNN 300

Query: 301 DEEASRFTVENFINGLQWLPETQIPFRGG 326
            +E + FTVE FING QWLP+T IPFR G
Sbjct: 301 AKEVATFTVEIFINGTQWLPQTGIPFRAG 329

[Close]

BLAST of Spg023974 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 290.0 bits (741), Expect = 2.3e-78
Identity = 141/313 (45.05%), Postives = 201/313 (64.22%), Query Frame = 0


Query: 24  TVSKDGTGNFARVSDAIAAAPNL---SSSRFYIHVKAGTYNEHVEIPEDKTFISLIGDGA 83
           TV ++GTGNF  ++ AIAAAPN    S+  F I+V AG Y E+VE+P++K ++ +IGDG 
Sbjct: 247 TVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGI 306

Query: 84  SNTVIVDGRNNHDGSSTTDSATFTVKGEHFIAQFITFENSAGPKKGQAVAFLDEANHTAY 143
           + TVI   R+  DG +T +SATF + G +FI   IT  N+AGP KGQAVA     + + +
Sbjct: 307 NQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVF 366

Query: 144 YNCVFKGYQDTLFVRGRFQFYKNCSVYGSVDFIFGEGLVVFQDCTIYARL----WGSTMT 203
           Y+C F+ YQDTL+     QFY+ C VYG+VDFIFG   VV Q+C +Y R       + +T
Sbjct: 367 YSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVT 426

Query: 204 AQSKSQVQDLSGFTFQNCNITTSSEMGSDKVTLS--LGRPWRSYSTVIVMQSFLDAVVEP 263
           AQ ++     +G     C I  + ++ +   T+   LGRPW+ YS  +VMQ+++D  +EP
Sbjct: 427 AQGRTDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEP 486

Query: 264 QGWSKWSG-IPINLLFYAEYNNYGPGANTSKRVNWPGYHILYTDEEASRFTVENFINGLQ 323
            GW+ WSG   ++ L+YAEYNN GPG++T+ RV WPGYH++    +AS FTV NF+ G  
Sbjct: 487 SGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVI-NATDASNFTVTNFLVGEG 546

Query: 324 WLPETQIPFRGGL 327
           W+ +T +PF GGL
Sbjct: 547 WIGQTGVPFVGGL 558

[Close]

BLAST of Spg023974 vs. TAIR 10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 290.0 bits (741), Expect = 2.3e-78
Identity = 144/312 (46.15%), Postives = 198/312 (63.46%), Query Frame = 0


Query: 21  FNSTVSKDGTGNFARVSDAIAAAPNLSSSRFYIHVKAGTYNEHVEIPEDKTFISLIGDGA 80
           +N +V+ DGTGNF  ++DA+ AAPN+S +RF I++K G Y E+VE+P+ KT I  IGDG 
Sbjct: 220 YNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGI 279

Query: 81  SNTVIVDGRNNHDGSSTTDSATFTVKGEHFIAQFITFENSAGPKKGQAVAFLDEANHTAY 140
             TVI   R+  DG ST  + T  VKG+ +IA+ I+F NSAGP K QAVAF   ++H+A+
Sbjct: 280 GKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSAF 339

Query: 141 YNCVFKGYQDTLFVRGRFQFYKNCSVYGSVDFIFGEGLVVFQDCTIYARLWGS----TMT 200
           Y C F GYQDTL+V    QFY+ C +YG++DFIFG   VVFQ+ ++YAR          T
Sbjct: 340 YRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAFT 399

Query: 201 AQSKSQVQDLSGFTFQNCNITTSSEMGSDKVTLS--LGRPWRSYSTVIVMQSFLDAVVEP 260
           AQS++Q    +G +  NC I  + ++   K      LGRPWR YS  ++++SF+D ++ P
Sbjct: 400 AQSRNQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIHP 459

Query: 261 QGWSKW-SGIPINLLFYAEYNNYGPGANTSKRVNWPGYHILYTDEEASRFTVENFINGLQ 320
            GW +      +  L+Y EY N GPGAN +KRV WPG+  +    EA++FTV  FI+G  
Sbjct: 460 AGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGST 519

Query: 321 WLPETQIPFRGG 326
           WL  T IPF  G
Sbjct: 520 WLNSTGIPFSLG 531

[Close]

BLAST of Spg023974 vs. TAIR 10
Match: AT2G26440.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 286.2 bits (731), Expect = 3.4e-77
Identity = 143/309 (46.28%), Postives = 199/309 (64.40%), Query Frame = 0


Query: 25  VSKDGTGNFARVSDAIAAAPNLSSSRFYIHVKAGTYNEHVEIPEDKTFISLIGDGASNTV 84
           V+ DGTGNF+ +++AI+ APN+S+ R  I+VK G Y+E+++IP  KT I LIGDG+  T 
Sbjct: 240 VAADGTGNFSTINEAISFAPNMSNDRVLIYVKEGVYDENIDIPIYKTNIVLIGDGSDVTF 299

Query: 85  IVDGRNNHDGSSTTDSATFTVKGEHFIAQFITFENSAGPKKGQAVAFLDEANHTAYYNCV 144
           I   R+  DG +T  SAT  V GE F+A+ I   N+AGP+K QAVA    A+  A Y CV
Sbjct: 300 ITGNRSVGDGWTTFRSATLAVSGEGFLARDIMITNTAGPEKHQAVALRVNADFVALYRCV 359

Query: 145 FKGYQDTLFVRGRFQFYKNCSVYGSVDFIFGEGLVVFQDCTIYARL----WGSTMTAQSK 204
             GYQDTL+     QFY+ C +YG++D+IFG   VVFQ C I ++L      + +TAQS+
Sbjct: 360 IDGYQDTLYTHSFRQFYRECDIYGTIDYIFGNAAVVFQGCNIVSKLPMPGQFTVITAQSR 419

Query: 205 SQVQDLSGFTFQNCNITTSSEM--GSDKVTLSLGRPWRSYSTVIVMQSFLDAVVEPQGWS 264
               + +G + QNC+I  S ++   S+KV   LGRPWR +S  +VM+S++D  ++  GWS
Sbjct: 420 DTQDEDTGISMQNCSILASEDLFNSSNKVKSYLGRPWREFSRTVVMESYIDEFIDGSGWS 479

Query: 265 KWS-GIPINLLFYAEYNNYGPGANTSKRVNWPGYHILYTDEEASRFTVENFINGLQWLPE 324
           KW+ G  ++ L+Y EYNN GPG+ T KRVNWPG+HI+   E+A  FT   FI G  WL  
Sbjct: 480 KWNGGEALDTLYYGEYNNNGPGSETVKRVNWPGFHIM-GYEDAFNFTATEFITGDGWLGS 539

Query: 325 TQIPFRGGL 327
           T  P+  G+
Sbjct: 540 TSFPYDNGI 547

[Close]

BLAST of Spg023974 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 285.8 bits (730), Expect = 4.4e-77
Identity = 140/313 (44.73%), Postives = 200/313 (63.90%), Query Frame = 0


Query: 24  TVSKDGTGNFARVSDAIAAAPNL---SSSRFYIHVKAGTYNEHVEIPEDKTFISLIGDGA 83
           TVS+DGTGNF  ++ A+AAAPN    S+  F I+V AG Y E++ I ++K ++ +IGDG 
Sbjct: 266 TVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIAKNKRYMMMIGDGI 325

Query: 84  SNTVIVDGRNNHDGSSTTDSATFTVKGEHFIAQFITFENSAGPKKGQAVAFLDEANHTAY 143
           + TV+   R+  DG +T +SATF V   +F+A  ITF N+AGP+K QAVA    A+ + +
Sbjct: 326 NQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAGPEKHQAVALRSGADFSIF 385

Query: 144 YNCVFKGYQDTLFVRGRFQFYKNCSVYGSVDFIFGEGLVVFQDCTIYARL----WGSTMT 203
           Y+C F+ YQDTL+     QFY+ C VYG+VDFIFG   VVFQ+C +Y R       + +T
Sbjct: 386 YSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAIT 445

Query: 204 AQSKSQVQDLSGFTFQNCNITTSSEMGSDKVTLS--LGRPWRSYSTVIVMQSFLDAVVEP 263
           AQ +S     +G + QNC I  + ++ S   T+   LGRPW+ YS  + MQS++D  VEP
Sbjct: 446 AQGRSDPNQNTGTSIQNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVYMQSYIDGFVEP 505

Query: 264 QGWSKWSG-IPINLLFYAEYNNYGPGANTSKRVNWPGYHILYTDEEASRFTVENFINGLQ 323
            GW +W+G   ++ L+YAEYNN GPG+NT+ RV WPGYH++    +A+ FTV        
Sbjct: 506 VGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVI-NSTDAANFTVTGLFIEAD 565

Query: 324 WLPETQIPFRGGL 327
           W+ +T +P+  GL
Sbjct: 566 WIWKTGVPYTSGL 577

[Close]

BLAST of Spg023974 vs. TAIR 10
Match: AT1G23200.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 283.5 bits (724), Expect = 2.2e-76
Identity = 140/309 (45.31%), Postives = 196/309 (63.43%), Query Frame = 0


Query: 25  VSKDGTGNFARVSDAIAAAPNL--SSSRFYIHVKAGTYNEHVEIPEDKTFISLIGDGASN 84
           V+KDG+G++  +  A+ AA  L   + R  I+VKAG Y E+V I +    + +IGDG  +
Sbjct: 253 VAKDGSGHYTSIQQAVNAAAKLPRRNQRLVIYVKAGVYRENVVIKKSIKNVMVIGDGIDS 312

Query: 85  TVIVDGRNNHDGSSTTDSATFTVKGEHFIAQFITFENSAGPKKGQAVAFLDEANHTAYYN 144
           T++   RN  DG++T  SATF V G  FIAQ ITFEN+AGP+K QAVA    ++ + +Y 
Sbjct: 313 TIVTGNRNVQDGTTTFRSATFAVSGNGFIAQGITFENTAGPEKHQAVALRSSSDFSVFYA 372

Query: 145 CVFKGYQDTLFVRGRFQFYKNCSVYGSVDFIFGEGLVVFQDCTIYARL----WGSTMTAQ 204
           C FKGYQDTL++    QF +NC++YG+VDFIFG+   + Q+C IYAR       +T+TAQ
Sbjct: 373 CSFKGYQDTLYLHSSRQFLRNCNIYGTVDFIFGDATAILQNCNIYARKPMSGQKNTITAQ 432

Query: 205 SKSQVQDLSGFTFQNCNITTSSEMGSDKVTLSLGRPWRSYSTVIVMQSFLDAVVEPQGWS 264
           S+ +  + +GF  Q+  + T+SE         LGRPWRS+S  + M+  L A+V P GW 
Sbjct: 433 SRKEPDETTGFVIQSSTVATASE-------TYLGRPWRSHSRTVFMKCNLGALVSPAGWL 492

Query: 265 KWSG-IPINLLFYAEYNNYGPGANTSKRVNWPGYHILYTDEEASRFTVENFINGLQWLPE 324
            WSG   ++ L+Y EY N G GA+ S RV WPGYH++ T  EA +FTVENF++G  W+  
Sbjct: 493 PWSGSFALSTLYYGEYGNTGAGASVSGRVKWPGYHVIKTVTEAEKFTVENFLDGNYWITA 552

Query: 325 TQIPFRGGL 327
           T +P   GL
Sbjct: 553 TGVPVNDGL 554

The following BLAST results are available for this feature:

BLAST of Spg023974 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022148123.1	2.1e-126	67.17	probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia]	[more]
XP_022150332.1	9.7e-119	63.06	probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia]	[more]
XP_023002625.1	1.6e-118	63.03	probable pectinesterase/pectinesterase inhibitor 39 [Cucurbita maxima]	[more]
XP_023538248.1	6.3e-118	63.03	probable pectinesterase/pectinesterase inhibitor 39 [Cucurbita pepo subsp. pepo]	[more]
XP_022951717.1	1.4e-117	62.73	probable pectinesterase/pectinesterase inhibitor 39 [Cucurbita moschata]	[more]

BLAST of Spg023974 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22256	3.3e-77	45.05	Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3...	[more]
O81415	3.3e-77	46.15	Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3...	[more]
Q43062	7.3e-77	45.31	Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica OX=3760 PE=2 SV=...	[more]
O04887	1.2e-76	43.73	Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1	[more]
O48711	4.7e-76	46.28	Probable pectinesterase/pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Spg023974 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D381	1.0e-126	67.17	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111016876 PE=4 SV=1	[more]
A0A6J1DAG5	4.7e-119	63.06	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018528 PE=4 SV=1	[more]
A0A6J1KPG6	8.0e-119	63.03	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111496427 PE=4 SV=1	[more]
A0A6J1GIG0	6.8e-118	62.73	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111454459 PE=4 SV=1	[more]
A0A0A0L1M3	3.1e-115	62.31	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_4G260430 PE=4 SV=1	[more]

BLAST of Spg023974 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G47550.1	2.3e-78	45.05	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G02300.1	2.3e-78	46.15	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT2G26440.1	3.4e-77	46.28	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT1G02810.1	4.4e-77	44.73	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT1G23200.1	2.2e-76	45.31	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 23..311 e-value: 3.8E-105 score: 350.9
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 21..326 e-value: 5.2E-110 score: 369.8
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 21..323
None	No IPR available	PANTHER	PTHR31707:SF3	PECTINESTERASE/PECTINESTERASE INHIBITOR 3	coord: 21..323
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 166..175
IPR018040	Pectinesterase, Tyr active site	PROSITE	PS00800	PECTINESTERASE_1	coord: 47..66
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 23..324

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg023974.1	Spg023974.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity