Spg023921 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg023921
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	cytochrome b5-like
Location	scaffold13: 5422322 .. 5426353 (-)
RNA-Seq Expression	Spg023921
Synteny	Spg023921

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAATTTAGATGTTTATTAAGTAAAAAAATTAAATTTAAAACCTCAATAAACACAAAAATGTGTACTAAAAAACACACAAACACAAAAATGAGATTTCCCAACATTGTTAAACACTTTGATGGAAATTTCAAACTCCTGAAAGTTGAAACACATACCTTAGCCAAAGCGAGTTTTGCTCAGTAGTAATTGACATGTACTATCGATTTCATCGACCATGAGGTTAGAGGTTCGAATCCCCCACTCCAAATGTTGATGTACTAGGGAAAAAAAAAAAAAAAAACTACCTTCCAATCCAAGTGTCGGCTGTTTGGACGCAAACCAAGAAACCGCTAGCGGCCAATAACTCACCCACCGCCCACCACCACAATTCAAATCCAATTCAATGCTTCCCCTTCACCCACATTGAAGCATTTACTCGCTTCCCTTTCCCCCCTTTATTTCTGTTCCGCCCTCACTGGTTGCTGCACTGCCCTTTTGCTTCACCTTCTCTGACCCTCAGCCCTTTTTGCTTCGTTTCTCCGCCGTCTCCGGGCAGATTGGCAAATGGGTAGCGACGGAAAGGTCTTCACATTGGCTGAAGTCTCTCAGCACAACAACCGTAAGGACTGTTGGTTGATCATCAATGCCAAGGTTTTTCTTCATACCCTTTTGTTTGTTTTTGGAGTTTCTTCTTTCAGATGCCTGATCATGTGTGGAGGAGTTTGATTCTGTGAATTTGTTTGTTCTTGTTCTGTTTTTTGTTTTCTATTGAAATCCCACATGCATTTTGGTCAGACGGACCCATTGTTTTTTCACAGTTGAATCGATGTGTTTAAACTGCTGCACTGTGAGTTTTGCTTCTGATGAACTGTATAGGCATGGGTAATGAAGGAATTTAGTTGCAGGTTATAGTGTACACAGAACATTTCCTTTACTTCTTTTGAGGCATGCAAAGAGATTCAATCCTTGGAGAGTGATGTTTCATCCTTATCAAGGTTTGGATGATTGGTTCTTTGGTCGCAGTTTTAAGCCAATTAAGTGGCTCTTAGTGTAGCAATTGCTGGTAATGGGGTAAGGCTCAAATGCCTCAGTTACATGGCTTGTTTAAATTGGAAAGCGTGAGTTTCAGAGTTTGTGTAATATGTGTTTGTAGATTATGGAAAGGAATGAATTGTTTGAATGTGGATTCATGTGACGTATGCTTGGTTTAGGGGCTTGTGATCCATTAAAGTTAATACATAGTGGATTTTATAAGCCCGAGTATGTGTAACCTATAGCCCATGAGATCAACAAATTTGTAGATTAACATAAACAAACTCACATGATAACAAACATAGGTTTGGCATCTTAATTCATAATTATACATGATTATACAAACTTAGGCCCTATTTGATAAACATTTAGTTTTTGTTTTTAATTTTTGATAATTAAGCTTATAAACATTACTTCCACCTATAAGTTTCTTTGTTTTGTTATCTACCTTCTTTCCATGTTTTCAAAAACCAAGTCCAGTAGTTTTCAAAAACCTGTTTTTGTTTATGGAAACCAACTAGAATTTAAATGTGTCATTCACAAAGATGAAAGCTATTGTAAGGAAATTGAGAAAACAAACATAATTCTAAAAAAACGAAAAACCAAATAATTATCAAACAGAGTCTTAATTACTTCTAAGTCAAACATTCCTTGCTCTGTGATAAGAATACCATTAGGAATGTAATTAGAGTATTAAGGGTATATTAGTAATTAGCTAGGGAGTTTGTTAGAGTATTTGGTTATAATTTATAAATAGAGGGAGTGGGAAAGGAGAAAGTTAGACACTGATTTAGTCCGTTCAAAGCTTGAGTGAGAGTGCTCAAGGAATGGAGGGTCCAAGTACTTAGAATTACTAGGTTTATTCTATAGCTTTTTACCTATTATGTTTCAATATCATCTTAGTTCTATTTAGTTTCTATCACTCTGCTCAGCTCACTGAGTTTATAAGACATGTTTGGAGATTTGAGATTTATGGAATTTAAAATGGACTGCGTTTAGTATTTTGAATGGCCTTATTTTGTCTTAGGACCGGGATCTCGTTGAAGACATAGAATCTGAGGTTTCTTTATATTTTTTTTAGAATTTAACGACTACTGAGGTTTCTTTATATTTGGCCATCCTTTTATGTAAAACAAATATCCTAGTTGATCGCTGTATTGGGTACTGCTTGATCCGTGTGATTCCCATATTTTCTATCAATGATCTGAAAATAATATCAGTTTAGGAAAACTTTGTGTGTTTTACATCTAAAAGTGGTTAATACGAGGAATGCTGATACAGAAAATGACCTCCAACTCTCATTACAATTTACTAGATCTCAATTTATGTTTGTGTGCGAATGCTCTTCTTTACTGAGGGCATTTTTGCTTCTTCAGTGACTGCTGGTAAATTTTATGCATACCTTGTGAGAACAGGACTCCAATGGGGGGTTGGACATCCAACACCTTGTTCAAACTTTACCTATAAAAAATTAGGTGTCTGGAGCTGACCCGGTCGGTTAATGTCGGCCACTGCAGCCCTTATCTACTATATAGATTTTGTTCATTCTACTGGTGCTATTTGATGACTTCTTCCTTTGCATCCATTGTGTTATCTGAAAGCAAAGTTATGATGCCTCACAATCTATTTAGGTTCTGCTGAATCTGCACATTCCATTGATATTCCACAGCCTGCTTTTTCGGTGTTAAAAATCTCTTACTGGGCGCCATACTCCTTTCATTTTCCTCCCTCCCTTGCTAATCTTCAAGGACGCTTCATCCAGTCCGAGGCCAAATCCAGGACATAATAAGGGCTTAGAATATTTTCAGAAAACAAGGATTTTAGAATATTAACTTTCTCACCGAGGGCCTTGAGCCTAGGCCTTGCTAGTGGTATCTCTAAACATCCAAGTCCTTACCACTAGAGCCATCCAAGGTAAAATCAGAAATTTACTGAACCCGAATTAATGATTCTAAGCTCTTGTTGGCTGTACTTGTTCATCATTGTAAATTGTAATATAAACTCTGCTTTCTTTCTCATTGATGTAATGTGAAATCTCTTTCTTGATCCACAATATCAGAAAGGGATGAAGATAGTGTAGGAATGCTTGAGACCCATGATCTTTAAACCAACTCAAGTTTCTTTAAGTGAAGAGTGAGAAGCAGGAAGACAAACATGAAAGAGTGCGAATCCTCAAATGAACTCGATTCCTACTCGGAAGCGAACATATAGAATTGCACAAACTCAATGAACCATGAATGATATCTTCAAAGAATTTTTTTTTTAGGATTTCTATTATGGTTTTCTTCTGAAATTTCTCAAAGAATAGTAAAAGAAAGTTAAATCATTCGTTCACCCCGGAATGCGTACAGCGAGCTTTTAGGCTAAGTGAGTCATGCTCAGATTATATATTTGATTGTTATTGATTTAGCTTGAAATTATCATTTTGTCTGTCTTGATCCCAACAGGTTTATAATGTAACCGAGTTCTTGGAAGACCATTCCGGTGGCGACGAGGTTTTGTTGTCAGGCACGGGTAATAAATCTTCATGTTGTATAGATTTAGTTCTGTGCATCGGTTTACCTAGCGTCAAATCTCCATCGCAACAGGTAAGGATGCAACGGATGATTTTGAAGATGTTGGTCATAGCGAGAGCGCAAGAGAAATGATGAACAAATACTACGTTGGAGAGATCGACACCTCGACGATCCCAGAAAGGTCGACATACACAGCTCCAAAGCAGCCTCACTACAATCAGGATAAGACAACGGAGTTCATCGTCAAGCTCCTCCAGTTCCTGGTTCCTCTCGCAATCTTGGGTATAGCAGTCGGTATCCGCTTTTACAGCAAATCAACCTGAGGTTCTTGTTGAGATATTAGGTGGTTGAGTCTCATTGTCAATTACAAACTGCTGCATTGTCTCTGTGAAACTTAGAAATTAATGTTGTTTTAGTTGAGAAGAATTCAAGATGGTTTTAGGACCTGTTTTCTGAATGGTAAAGTTAATGTAATCATGTAGTTGGTGATTTCATCAGTATTTT

mRNA sequence

ATGGGTAGCGACGGAAAGGTCTTCACATTGGCTGAAGTCTCTCAGCACAACAACCGTAAGGACTGTTGGTTGATCATCAATGCCAAGGACCGGGATCTCGTTGAAGACATAGAATCTGAGGTTTATAATGTAACCGAGTTCTTGGAAGACCATTCCGGTGGCGACGAGGTTTTGTTGTCAGGCACGGGTAAGGATGCAACGGATGATTTTGAAGATGTTGGTCATAGCGAGAGCGCAAGAGAAATGATGAACAAATACTACGTTGGAGAGATCGACACCTCGACGATCCCAGAAAGGTCGACATACACAGCTCCAAAGCAGCCTCACTACAATCAGGATAAGACAACGGAGTTCATCGTCAAGCTCCTCCAGTTCCTGGTTCCTCTCGCAATCTTGGGTATAGCAGTCGGTATCCGCTTTTACAGCAAATCAACCTGA

Coding sequence (CDS)

ATGGGTAGCGACGGAAAGGTCTTCACATTGGCTGAAGTCTCTCAGCACAACAACCGTAAGGACTGTTGGTTGATCATCAATGCCAAGGACCGGGATCTCGTTGAAGACATAGAATCTGAGGTTTATAATGTAACCGAGTTCTTGGAAGACCATTCCGGTGGCGACGAGGTTTTGTTGTCAGGCACGGGTAAGGATGCAACGGATGATTTTGAAGATGTTGGTCATAGCGAGAGCGCAAGAGAAATGATGAACAAATACTACGTTGGAGAGATCGACACCTCGACGATCCCAGAAAGGTCGACATACACAGCTCCAAAGCAGCCTCACTACAATCAGGATAAGACAACGGAGTTCATCGTCAAGCTCCTCCAGTTCCTGGTTCCTCTCGCAATCTTGGGTATAGCAGTCGGTATCCGCTTTTACAGCAAATCAACCTGA

Protein sequence

MGSDGKVFTLAEVSQHNNRKDCWLIINAKDRDLVEDIESEVYNVTEFLEDHSGGDEVLLSGTGKDATDDFEDVGHSESAREMMNKYYVGEIDTSTIPERSTYTAPKQPHYNQDKTTEFIVKLLQFLVPLAILGIAVGIRFYSKST

Homology

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BLAST of Spg023921 vs. NCBI nr
Match: XP_023006481.1 (cytochrome b5-like [Cucurbita maxima])

HSP 1 Score: 242.7 bits (618), Expect = 2.0e-60
Identity = 123/145 (84.83%), Postives = 125/145 (86.21%), Query Frame = 0


Query: 1   MGSDGKVFTLAEVSQHNNRKDCWLIINAKDRDLVEDIESEVYNVTEFLEDHSGGDEVLLS 60
           MG  GKVFTLAEVSQHN R+DCWLIIN K           VYNVTEFLEDH GGDEVLLS
Sbjct: 1   MGGHGKVFTLAEVSQHNTRQDCWLIINGK-----------VYNVTEFLEDHPGGDEVLLS 60

Query: 61  GTGKDATDDFEDVGHSESAREMMNKYYVGEIDTSTIPERSTYTAPKQPHYNQDKTTEFIV 120
           GTGKDATDDFEDVGHSESARE MNKYYVGEID STIPER TYTA KQPHYNQDKTTEFIV
Sbjct: 61  GTGKDATDDFEDVGHSESARESMNKYYVGEIDISTIPERLTYTASKQPHYNQDKTTEFIV 120

Query: 121 KLLQFLVPLAILGIAVGIRFYSKST 146
           KLLQFLVPLAILG+AVGIRFYSKST
Sbjct: 121 KLLQFLVPLAILGLAVGIRFYSKST 134

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BLAST of Spg023921 vs. NCBI nr
Match: XP_022959033.1 (cytochrome b5-like [Cucurbita moschata])

HSP 1 Score: 241.5 bits (615), Expect = 4.5e-60
Identity = 122/145 (84.14%), Postives = 125/145 (86.21%), Query Frame = 0


Query: 1   MGSDGKVFTLAEVSQHNNRKDCWLIINAKDRDLVEDIESEVYNVTEFLEDHSGGDEVLLS 60
           MG  GKVFTLAEVSQHN R+DCWLIIN K           VYNVTEFLEDH GGDEVLLS
Sbjct: 1   MGGHGKVFTLAEVSQHNTRQDCWLIINGK-----------VYNVTEFLEDHPGGDEVLLS 60

Query: 61  GTGKDATDDFEDVGHSESAREMMNKYYVGEIDTSTIPERSTYTAPKQPHYNQDKTTEFIV 120
           GTGKDATDDFEDVGHSESARE MNKYYVG+ID STIPER TYTA KQPHYNQDKTTEFIV
Sbjct: 61  GTGKDATDDFEDVGHSESARETMNKYYVGDIDISTIPERLTYTASKQPHYNQDKTTEFIV 120

Query: 121 KLLQFLVPLAILGIAVGIRFYSKST 146
           KLLQFLVPLAILG+AVGIRFYSKST
Sbjct: 121 KLLQFLVPLAILGLAVGIRFYSKST 134

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BLAST of Spg023921 vs. NCBI nr
Match: XP_008457249.1 (PREDICTED: cytochrome b5-like [Cucumis melo] >KAA0033284.1 cytochrome b5-like [Cucumis melo var. makuwa] >TYK14823.1 cytochrome b5-like [Cucumis melo var. makuwa])

HSP 1 Score: 241.1 bits (614), Expect = 5.9e-60
Identity = 118/145 (81.38%), Postives = 127/145 (87.59%), Query Frame = 0


Query: 1   MGSDGKVFTLAEVSQHNNRKDCWLIINAKDRDLVEDIESEVYNVTEFLEDHSGGDEVLLS 60
           MG DGK++TLAEVSQHNN KDCWLIIN K           VYNVTEFLEDH GGDEVLLS
Sbjct: 1   MGGDGKLYTLAEVSQHNNPKDCWLIINGK-----------VYNVTEFLEDHPGGDEVLLS 60

Query: 61  GTGKDATDDFEDVGHSESAREMMNKYYVGEIDTSTIPERSTYTAPKQPHYNQDKTTEFIV 120
           GTGKDATDDFEDVGHSESAREMM+KYY+GEID STIPE+ TYT PKQPHYNQDKTTEFI+
Sbjct: 61  GTGKDATDDFEDVGHSESAREMMSKYYIGEIDISTIPEQLTYTGPKQPHYNQDKTTEFII 120

Query: 121 KLLQFLVPLAILGIAVGIRFYSKST 146
           KLLQFLVPLAILG+AVGIRFY+KS+
Sbjct: 121 KLLQFLVPLAILGLAVGIRFYNKSS 134

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BLAST of Spg023921 vs. NCBI nr
Match: KAG6574866.1 (Cytochrome b5 isoform B, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013438.1 Cytochrome b5 isoform B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 240.4 bits (612), Expect = 1.0e-59
Identity = 121/145 (83.45%), Postives = 125/145 (86.21%), Query Frame = 0


Query: 1   MGSDGKVFTLAEVSQHNNRKDCWLIINAKDRDLVEDIESEVYNVTEFLEDHSGGDEVLLS 60
           MG  GKVFTLAEVSQHN R+DCWLIIN K           VYNVTEFLEDH GGDEVLL+
Sbjct: 1   MGGHGKVFTLAEVSQHNTRQDCWLIINGK-----------VYNVTEFLEDHPGGDEVLLT 60

Query: 61  GTGKDATDDFEDVGHSESAREMMNKYYVGEIDTSTIPERSTYTAPKQPHYNQDKTTEFIV 120
           GTGKDATDDFEDVGHSESARE MNKYYVG+ID STIPER TYTA KQPHYNQDKTTEFIV
Sbjct: 61  GTGKDATDDFEDVGHSESARETMNKYYVGDIDISTIPERLTYTASKQPHYNQDKTTEFIV 120

Query: 121 KLLQFLVPLAILGIAVGIRFYSKST 146
           KLLQFLVPLAILG+AVGIRFYSKST
Sbjct: 121 KLLQFLVPLAILGLAVGIRFYSKST 134

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BLAST of Spg023921 vs. NCBI nr
Match: XP_038906915.1 (cytochrome b5-like [Benincasa hispida])

HSP 1 Score: 239.6 bits (610), Expect = 1.7e-59
Identity = 121/145 (83.45%), Postives = 125/145 (86.21%), Query Frame = 0


Query: 1   MGSDGKVFTLAEVSQHNNRKDCWLIINAKDRDLVEDIESEVYNVTEFLEDHSGGDEVLLS 60
           M  D K+FTLAEVSQHNNRKDCWLIIN K           VYNVTEFLEDH GGDEVLLS
Sbjct: 1   MDGDRKLFTLAEVSQHNNRKDCWLIINGK-----------VYNVTEFLEDHPGGDEVLLS 60

Query: 61  GTGKDATDDFEDVGHSESAREMMNKYYVGEIDTSTIPERSTYTAPKQPHYNQDKTTEFIV 120
           GTGKDATDDFEDVGHSESAREMM+KY VGEID STIPERSTYT  KQPHYNQDKTTEFI+
Sbjct: 61  GTGKDATDDFEDVGHSESAREMMSKYSVGEIDISTIPERSTYTTSKQPHYNQDKTTEFII 120

Query: 121 KLLQFLVPLAILGIAVGIRFYSKST 146
           KLLQFLVPLAILG+AVGIRFYSKST
Sbjct: 121 KLLQFLVPLAILGLAVGIRFYSKST 134

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BLAST of Spg023921 vs. ExPASy Swiss-Prot
Match: O48845 (Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 3.5e-55
Identity = 103/144 (71.53%), Postives = 119/144 (82.64%), Query Frame = 0


Query: 1   MGSDGKVFTLAEVSQHNNRKDCWLIINAKDRDLVEDIESEVYNVTEFLEDHSGGDEVLLS 60
           MG + K+FTL+EVS+HN   DCW++IN K           VYNVT+FLEDH GGD+VLLS
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGK-----------VYNVTKFLEDHPGGDDVLLS 60

Query: 61  GTGKDATDDFEDVGHSESAREMMNKYYVGEIDTSTIPERSTYTAPKQPHYNQDKTTEFIV 120
            TGKDATDDFEDVGHSESAREMM +YYVGEID +TIP++  YT PKQPHYNQDKT+EFI+
Sbjct: 61  STGKDATDDFEDVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFII 120

Query: 121 KLLQFLVPLAILGIAVGIRFYSKS 145
           KLLQFLVPLAILG+AVGIR Y+KS
Sbjct: 121 KLLQFLVPLAILGLAVGIRIYTKS 133

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BLAST of Spg023921 vs. ExPASy Swiss-Prot
Match: P49099 (Cytochrome b5, seed isoform OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 1.9e-53
Identity = 102/145 (70.34%), Postives = 115/145 (79.31%), Query Frame = 0


Query: 1   MGSDGKVFTLAEVSQHNNRKDCWLIINAKDRDLVEDIESEVYNVTEFLEDHSGGDEVLLS 60
           MG   KVFTLAEVS HNN KDCWLII+ K           VYNVT+FLEDH GG EVLLS
Sbjct: 1   MGGQSKVFTLAEVSNHNNAKDCWLIISGK-----------VYNVTKFLEDHPGGGEVLLS 60

Query: 61  GTGKDATDDFEDVGHSESAREMMNKYYVGEIDTSTIPERSTYTAPKQPHYNQDKTTEFIV 120
            TGKDATDDFED+GHS SAR M+++YYVG+ID+STIP +  YT PKQPHYNQDKTTEFIV
Sbjct: 61  ATGKDATDDFEDIGHSSSARAMLDEYYVGDIDSSTIPTKVKYTPPKQPHYNQDKTTEFIV 120

Query: 121 KLLQFLVPLAILGIAVGIRFYSKST 146
           KLLQFLVPL ILG+A G+ FY+K +
Sbjct: 121 KLLQFLVPLIILGVAFGVHFYTKQS 134

[Close]

BLAST of Spg023921 vs. ExPASy Swiss-Prot
Match: P49098 (Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 1.9e-53
Identity = 100/145 (68.97%), Postives = 119/145 (82.07%), Query Frame = 0


Query: 1   MGSDGKVFTLAEVSQHNNRKDCWLIINAKDRDLVEDIESEVYNVTEFLEDHSGGDEVLLS 60
           MG + KVFTLAEVSQHNN KDCWL+I+ K           VY+VT+FL+DH GGDEVLLS
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGK-----------VYDVTKFLDDHPGGDEVLLS 60

Query: 61  GTGKDATDDFEDVGHSESAREMMNKYYVGEIDTSTIPERSTYTAPKQPHYNQDKTTEFIV 120
            TGKDATDDFEDVGHS SAR M+++YYVG+ID++TIP ++ YT P QPHYNQDKT+EF+V
Sbjct: 61  ATGKDATDDFEDVGHSSSARAMLDEYYVGDIDSATIPTKTKYTPPNQPHYNQDKTSEFVV 120

Query: 121 KLLQFLVPLAILGIAVGIRFYSKST 146
           KLLQFLVPL ILG+A GIRFY+K +
Sbjct: 121 KLLQFLVPLIILGVAFGIRFYTKQS 134

[Close]

BLAST of Spg023921 vs. ExPASy Swiss-Prot
Match: P49100 (Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 198.7 bits (504), Expect = 4.4e-50
Identity = 96/139 (69.06%), Postives = 113/139 (81.29%), Query Frame = 0


Query: 6   KVFTLAEVSQHNNRKDCWLIINAKDRDLVEDIESEVYNVTEFLEDHSGGDEVLLSGTGKD 65
           KV+TL EV++HN++ DCWLII  K           VYNV++FLEDH GGD+VLLS TGKD
Sbjct: 7   KVYTLEEVAKHNSKDDCWLIIGGK-----------VYNVSKFLEDHPGGDDVLLSSTGKD 66

Query: 66  ATDDFEDVGHSESAREMMNKYYVGEIDTSTIPERSTYTAPKQPHYNQDKTTEFIVKLLQF 125
           ATDDFEDVGHS +AR MM++YYVG+IDTSTIP R+ Y  PKQPHYNQDKT EFI+K+LQF
Sbjct: 67  ATDDFEDVGHSTTARAMMDEYYVGDIDTSTIPARTKYVPPKQPHYNQDKTPEFIIKILQF 126

Query: 126 LVPLAILGIAVGIRFYSKS 145
           LVPLAILG+AV IR Y+KS
Sbjct: 127 LVPLAILGLAVAIRIYTKS 134

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BLAST of Spg023921 vs. ExPASy Swiss-Prot
Match: O04354 (Cytochrome b5 OS=Borago officinalis OX=13363 PE=2 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 5.7e-50
Identity = 95/141 (67.38%), Postives = 114/141 (80.85%), Query Frame = 0


Query: 5   GKVFTLAEVSQHNNRKDCWLIINAKDRDLVEDIESEVYNVTEFLEDHSGGDEVLLSGTGK 64
           GK+FTLAEV+QHNN KDCWLIIN K           VY+VT+FLEDH GGD+VLLS TGK
Sbjct: 2   GKIFTLAEVAQHNNSKDCWLIINGK-----------VYDVTKFLEDHPGGDDVLLSATGK 61

Query: 65  DATDDFEDVGHSESAREMMNKYYVGEIDTSTIPERSTYTAPKQPHYNQDKTTEFIVKLLQ 124
           DATDDFED+GHS SA+ M+++YYVG+ID+S+IP +  YT PKQP YN DKT EF++KLLQ
Sbjct: 62  DATDDFEDIGHSSSAKAMLDEYYVGDIDSSSIPSQVKYTPPKQPLYNPDKTREFVIKLLQ 121

Query: 125 FLVPLAILGIAVGIRFYSKST 146
           FLVPL IL  A+GIRFY+KS+
Sbjct: 122 FLVPLVILAGAIGIRFYTKSS 131

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BLAST of Spg023921 vs. ExPASy TrEMBL
Match: A0A6J1KXV9 (cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111499200 PE=3 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 9.8e-61
Identity = 123/145 (84.83%), Postives = 125/145 (86.21%), Query Frame = 0


Query: 1   MGSDGKVFTLAEVSQHNNRKDCWLIINAKDRDLVEDIESEVYNVTEFLEDHSGGDEVLLS 60
           MG  GKVFTLAEVSQHN R+DCWLIIN K           VYNVTEFLEDH GGDEVLLS
Sbjct: 1   MGGHGKVFTLAEVSQHNTRQDCWLIINGK-----------VYNVTEFLEDHPGGDEVLLS 60

Query: 61  GTGKDATDDFEDVGHSESAREMMNKYYVGEIDTSTIPERSTYTAPKQPHYNQDKTTEFIV 120
           GTGKDATDDFEDVGHSESARE MNKYYVGEID STIPER TYTA KQPHYNQDKTTEFIV
Sbjct: 61  GTGKDATDDFEDVGHSESARESMNKYYVGEIDISTIPERLTYTASKQPHYNQDKTTEFIV 120

Query: 121 KLLQFLVPLAILGIAVGIRFYSKST 146
           KLLQFLVPLAILG+AVGIRFYSKST
Sbjct: 121 KLLQFLVPLAILGLAVGIRFYSKST 134

[Close]

BLAST of Spg023921 vs. ExPASy TrEMBL
Match: A0A6J1H4S5 (cytochrome b5-like OS=Cucurbita moschata OX=3662 GN=LOC111460140 PE=3 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 2.2e-60
Identity = 122/145 (84.14%), Postives = 125/145 (86.21%), Query Frame = 0


Query: 1   MGSDGKVFTLAEVSQHNNRKDCWLIINAKDRDLVEDIESEVYNVTEFLEDHSGGDEVLLS 60
           MG  GKVFTLAEVSQHN R+DCWLIIN K           VYNVTEFLEDH GGDEVLLS
Sbjct: 1   MGGHGKVFTLAEVSQHNTRQDCWLIINGK-----------VYNVTEFLEDHPGGDEVLLS 60

Query: 61  GTGKDATDDFEDVGHSESAREMMNKYYVGEIDTSTIPERSTYTAPKQPHYNQDKTTEFIV 120
           GTGKDATDDFEDVGHSESARE MNKYYVG+ID STIPER TYTA KQPHYNQDKTTEFIV
Sbjct: 61  GTGKDATDDFEDVGHSESARETMNKYYVGDIDISTIPERLTYTASKQPHYNQDKTTEFIV 120

Query: 121 KLLQFLVPLAILGIAVGIRFYSKST 146
           KLLQFLVPLAILG+AVGIRFYSKST
Sbjct: 121 KLLQFLVPLAILGLAVGIRFYSKST 134

[Close]

BLAST of Spg023921 vs. ExPASy TrEMBL
Match: A0A5D3CSF5 (Cytochrome b5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold472G00160 PE=3 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 2.8e-60
Identity = 118/145 (81.38%), Postives = 127/145 (87.59%), Query Frame = 0


Query: 1   MGSDGKVFTLAEVSQHNNRKDCWLIINAKDRDLVEDIESEVYNVTEFLEDHSGGDEVLLS 60
           MG DGK++TLAEVSQHNN KDCWLIIN K           VYNVTEFLEDH GGDEVLLS
Sbjct: 1   MGGDGKLYTLAEVSQHNNPKDCWLIINGK-----------VYNVTEFLEDHPGGDEVLLS 60

Query: 61  GTGKDATDDFEDVGHSESAREMMNKYYVGEIDTSTIPERSTYTAPKQPHYNQDKTTEFIV 120
           GTGKDATDDFEDVGHSESAREMM+KYY+GEID STIPE+ TYT PKQPHYNQDKTTEFI+
Sbjct: 61  GTGKDATDDFEDVGHSESAREMMSKYYIGEIDISTIPEQLTYTGPKQPHYNQDKTTEFII 120

Query: 121 KLLQFLVPLAILGIAVGIRFYSKST 146
           KLLQFLVPLAILG+AVGIRFY+KS+
Sbjct: 121 KLLQFLVPLAILGLAVGIRFYNKSS 134

[Close]

BLAST of Spg023921 vs. ExPASy TrEMBL
Match: A0A1S3C5R9 (cytochrome b5-like OS=Cucumis melo OX=3656 GN=LOC103496974 PE=3 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 2.8e-60
Identity = 118/145 (81.38%), Postives = 127/145 (87.59%), Query Frame = 0


Query: 1   MGSDGKVFTLAEVSQHNNRKDCWLIINAKDRDLVEDIESEVYNVTEFLEDHSGGDEVLLS 60
           MG DGK++TLAEVSQHNN KDCWLIIN K           VYNVTEFLEDH GGDEVLLS
Sbjct: 1   MGGDGKLYTLAEVSQHNNPKDCWLIINGK-----------VYNVTEFLEDHPGGDEVLLS 60

Query: 61  GTGKDATDDFEDVGHSESAREMMNKYYVGEIDTSTIPERSTYTAPKQPHYNQDKTTEFIV 120
           GTGKDATDDFEDVGHSESAREMM+KYY+GEID STIPE+ TYT PKQPHYNQDKTTEFI+
Sbjct: 61  GTGKDATDDFEDVGHSESAREMMSKYYIGEIDISTIPEQLTYTGPKQPHYNQDKTTEFII 120

Query: 121 KLLQFLVPLAILGIAVGIRFYSKST 146
           KLLQFLVPLAILG+AVGIRFY+KS+
Sbjct: 121 KLLQFLVPLAILGLAVGIRFYNKSS 134

[Close]

BLAST of Spg023921 vs. ExPASy TrEMBL
Match: A0A0A0LHS9 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G138200 PE=3 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 8.3e-60
Identity = 118/145 (81.38%), Postives = 125/145 (86.21%), Query Frame = 0


Query: 1   MGSDGKVFTLAEVSQHNNRKDCWLIINAKDRDLVEDIESEVYNVTEFLEDHSGGDEVLLS 60
           MG DGK+FT AEVSQHNN KDCWLIIN K           VYNVTEFLEDH GGDEVLLS
Sbjct: 1   MGGDGKLFTFAEVSQHNNPKDCWLIINGK-----------VYNVTEFLEDHPGGDEVLLS 60

Query: 61  GTGKDATDDFEDVGHSESAREMMNKYYVGEIDTSTIPERSTYTAPKQPHYNQDKTTEFIV 120
           GTGKDATDDFEDVGHSESARE M+KYYVGEID ST+PER TYT PKQPHYNQDKTTEFI+
Sbjct: 61  GTGKDATDDFEDVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFII 120

Query: 121 KLLQFLVPLAILGIAVGIRFYSKST 146
           KLLQFLVPLAILG+AVGIRFY+KS+
Sbjct: 121 KLLQFLVPLAILGLAVGIRFYNKSS 134

[Close]

BLAST of Spg023921 vs. TAIR 10
Match: AT2G32720.1 (cytochrome B5 isoform B )

HSP 1 Score: 215.7 bits (548), Expect = 2.5e-56
Identity = 103/144 (71.53%), Postives = 119/144 (82.64%), Query Frame = 0


Query: 1   MGSDGKVFTLAEVSQHNNRKDCWLIINAKDRDLVEDIESEVYNVTEFLEDHSGGDEVLLS 60
           MG + K+FTL+EVS+HN   DCW++IN K           VYNVT+FLEDH GGD+VLLS
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGK-----------VYNVTKFLEDHPGGDDVLLS 60

Query: 61  GTGKDATDDFEDVGHSESAREMMNKYYVGEIDTSTIPERSTYTAPKQPHYNQDKTTEFIV 120
            TGKDATDDFEDVGHSESAREMM +YYVGEID +TIP++  YT PKQPHYNQDKT+EFI+
Sbjct: 61  STGKDATDDFEDVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFII 120

Query: 121 KLLQFLVPLAILGIAVGIRFYSKS 145
           KLLQFLVPLAILG+AVGIR Y+KS
Sbjct: 121 KLLQFLVPLAILGLAVGIRIYTKS 133

[Close]

BLAST of Spg023921 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 186.4 bits (472), Expect = 1.6e-47
Identity = 88/143 (61.54%), Postives = 112/143 (78.32%), Query Frame = 0


Query: 1   MGSDGKVFTLAEVSQHNNRKDCWLIINAKDRDLVEDIESEVYNVTEFLEDHSGGDEVLLS 60
           M SD KV +  EVS+HN  KDCWLII+ K           VY+VT F++DH GGDEVLLS
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGK-----------VYDVTPFMDDHPGGDEVLLS 60

Query: 61  GTGKDATDDFEDVGHSESAREMMNKYYVGEIDTSTIPERSTYTAPKQPHYNQDKTTEFIV 120
            TGKDAT+DFEDVGHS++AR+MM+KY++GEID+S++P   TY AP+QP YNQDKT EFI+
Sbjct: 61  STGKDATNDFEDVGHSDTARDMMDKYFIGEIDSSSVPATRTYVAPQQPAYNQDKTPEFII 120

Query: 121 KLLQFLVPLAILGIAVGIRFYSK 144
           K+LQFLVP+ ILG+A+ +R Y+K
Sbjct: 121 KILQFLVPILILGLALVVRHYTK 132

[Close]

BLAST of Spg023921 vs. TAIR 10
Match: AT5G48810.1 (cytochrome B5 isoform D )

HSP 1 Score: 177.6 bits (449), Expect = 7.4e-45
Identity = 83/146 (56.85%), Postives = 114/146 (78.08%), Query Frame = 0


Query: 1   MGSDGKVFTLAEVSQHNNRKDCWLIINAKDRDLVEDIESEVYNVTEFLEDHSGGDEVLLS 60
           MG DGKVFTL+EVSQH++ KDCW++I+ K           VY+VT+FL+DH GGDEV+L+
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGK-----------VYDVTKFLDDHPGGDEVILT 60

Query: 61  GTGKDATDDFEDVGHSESAREMMNKYYVGEIDTSTIPERSTYTAPKQPH--YNQDKTTEF 120
            TGKDATDDFEDVGHS +A+ M+++YYVG+IDT+T+P ++ +  P        QDK+++F
Sbjct: 61  STGKDATDDFEDVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAVATQDKSSDF 120

Query: 121 IVKLLQFLVPLAILGIAVGIRFYSKS 145
           ++KLLQFLVPL ILG+A GIR+Y+K+
Sbjct: 121 VIKLLQFLVPLLILGLAFGIRYYTKT 135

[Close]

BLAST of Spg023921 vs. TAIR 10
Match: AT2G46650.1 (cytochrome B5 isoform C )

HSP 1 Score: 115.2 bits (287), Expect = 4.5e-26
Identity = 57/133 (42.86%), Postives = 86/133 (64.66%), Query Frame = 0


Query: 12  EVSQHNNRKDCWLIINAKDRDLVEDIESEVYNVTEFLEDHSGGDEVLLSGTGKDATDDFE 71
           +V++H  + DCW++I+ K           VY+++ F+++H GGD VLL+ TGKDA+ DFE
Sbjct: 9   DVAKHKCKNDCWILIHGK-----------VYDISTFMDEHPGGDNVLLAVTGKDASIDFE 68

Query: 72  DVGHSESAREMMNKYYVGEIDTSTIPERSTYTAPKQPHYNQDKTT--EFIVKLLQFLVPL 131
           DV HS+ A+E+M KY +G++D ST+P    Y  P +      +TT  E   KLL +L+PL
Sbjct: 69  DVNHSKDAKELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTKEESGKKLLIYLIPL 128

Query: 132 AILGIAVGIRFYS 143
            ILG+A  +RFY+
Sbjct: 129 LILGVAFALRFYN 130

[Close]

BLAST of Spg023921 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 114.4 bits (285), Expect = 7.7e-26
Identity = 56/146 (38.36%), Postives = 94/146 (64.38%), Query Frame = 0


Query: 6   KVFTLAEVSQHNNRKDCWLIINAKDRDLVEDIESEVYNVTEFLEDHSGGDEVLLSGTGKD 65
           K++++ E + HN + DCW++I+ K           VY+V+ ++++H GGD+VLL+  GKD
Sbjct: 6   KLYSMEEAATHNKQDDCWVVIDGK-----------VYDVSSYMDEHPGGDDVLLAVAGKD 65

Query: 66  ATDDFEDVGHSESAREMMNKYYVGEIDTSTIPERSTYTAPKQPHYNQDKTTEFIVKLLQ- 125
           ATDDFED GHS+ ARE+M KY++GE+D S++PE      P+   Y +D+  + + KL   
Sbjct: 66  ATDDFEDAGHSKDARELMEKYFIGELDESSLPE-----IPELKIYKKDQPQDSVQKLFDL 125

Query: 126 ----FLVPLAILGIAVGIR-FYSKST 146
               ++VP++I+ I+V +   +S+ T
Sbjct: 126 TKQYWVVPVSIITISVAVSVLFSRKT 135

The following BLAST results are available for this feature:

BLAST of Spg023921 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023006481.1	2.0e-60	84.83	cytochrome b5-like [Cucurbita maxima]	[more]
XP_022959033.1	4.5e-60	84.14	cytochrome b5-like [Cucurbita moschata]	[more]
XP_008457249.1	5.9e-60	81.38	PREDICTED: cytochrome b5-like [Cucumis melo] >KAA0033284.1 cytochrome b5-like [C...	[more]
KAG6574866.1	1.0e-59	83.45	Cytochrome b5 isoform B, partial [Cucurbita argyrosperma subsp. sororia] >KAG701...	[more]
XP_038906915.1	1.7e-59	83.45	cytochrome b5-like [Benincasa hispida]	[more]

BLAST of Spg023921 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O48845	3.5e-55	71.53	Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1	[more]
P49099	1.9e-53	70.34	Cytochrome b5, seed isoform OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
P49098	1.9e-53	68.97	Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
P49100	4.4e-50	69.06	Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2	[more]
O04354	5.7e-50	67.38	Cytochrome b5 OS=Borago officinalis OX=13363 PE=2 SV=1	[more]

BLAST of Spg023921 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KXV9	9.8e-61	84.83	cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111499200 PE=3 SV=1	[more]
A0A6J1H4S5	2.2e-60	84.14	cytochrome b5-like OS=Cucurbita moschata OX=3662 GN=LOC111460140 PE=3 SV=1	[more]
A0A5D3CSF5	2.8e-60	81.38	Cytochrome b5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold472G0...	[more]
A0A1S3C5R9	2.8e-60	81.38	cytochrome b5-like OS=Cucumis melo OX=3656 GN=LOC103496974 PE=3 SV=1	[more]
A0A0A0LHS9	8.3e-60	81.38	Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ...	[more]

BLAST of Spg023921 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G32720.1	2.5e-56	71.53	cytochrome B5 isoform B	[more]
AT5G53560.1	1.6e-47	61.54	cytochrome B5 isoform E	[more]
AT5G48810.1	7.4e-45	56.85	cytochrome B5 isoform D	[more]
AT2G46650.1	4.5e-26	42.86	cytochrome B5 isoform C	[more]
AT1G26340.1	7.7e-26	38.36	cytochrome B5 isoform A	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 51..65 score: 46.87 coord: 66..73 score: 48.11 coord: 41..51 score: 61.02 coord: 79..91 score: 39.16
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 8..92 e-value: 2.2E-28 score: 110.3
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 9..91 e-value: 9.8E-20 score: 70.5
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 5..92 score: 26.68
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 2..97 e-value: 2.1E-31 score: 109.7
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 6..99
None	No IPR available	PIRSR	PIRSR005149-50	PIRSR005149-50	coord: 6..103 e-value: 8.1E-15 score: 52.4
None	No IPR available	PANTHER	PTHR19359:SF110	BNAC04G43300D PROTEIN	coord: 1..143
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 1..143

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg023921.1	Spg023921.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043447	alkane biosynthetic process
biological_process	GO:0042761	very long-chain fatty acid biosynthetic process
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0043231	intracellular membrane-bounded organelle
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding