Spg023860 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg023860
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Ent-kaurene oxidase
Location	scaffold13: 6237480 .. 6238272 (-)
RNA-Seq Expression	Spg023860
Synteny	Spg023860

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAGTTTGAACTTGATGTCTATTTGGTCCCTGAAGTTTAAAAAGTGACACTTTAGTCCCTGAGGTTTAAAAAATACTTCTAAATAGTCCATGAGTTAATTTGACCGTTAGTTGACTTAACGAAAAAATGATGTGGCATGATGAGGTGGCACATTTTAATTGAGGTGGCCAATTTAATAATTTTATATTATTTTTTTCTTTTTCTTTTTCTTTTTTTTTTCCTCTTTCTCTTTCTCTCTTCTCTTATCTTCCCTTCTTCCCTCTCTTTATCTCTCTGTTTTTGAATCATCGGTGGAGTTGATCAATTGGGGTTTTTAGGGAAAAATGATGTGAAGTTTTTGATCTAATGGATTTGAGAATGGTGGGTTTGCAGCGATTCCGGGAGTGCCGGTGCTGGGGAATTTGTTGCAGTTGAAGGAGAAGAAGCCTCACAAGACGTTTTCTCAGTGGGCCGAGACGTACGGTCCGGTTTATTCTATAAGAACCGGCGCTTCCACTGTGGTTGTTCTCAACACTGCCGAGGTCGCCAAAGAGGTTCAAACCACCGCCGTTTTCCGGCGAACTTTTGAACTCTGAATGAAAAAGTTGTCTCTGATTTTAATCGTACTCTGTTTTGGTCTTGATTCTTTAAGGCTATGGTGACGAGATATTCCTCCATTTCCAGTAGAAAGCTAAGCAAAGCCCTCACCATTTTGACTTCTGATAAATGCATGGTTGCTATGAGTGATTACAATGACTTTCACAAGTTGGTGAAGAGATATATATTGGCTAATCTCTTGGGTGTTAATGCTCAG

mRNA sequence

ATGGTGACGAGATATTCCTCCATTTCCAGTAGAAAGCTAAGCAAAGCCCTCACCATTTTGACTTCTGATAAATGCATGGTTGCTATGAGTGATTACAATGACTTTCACAAGTTGGTGAAGAGATATATATTGGCTAATCTCTTGGGTGTTAATGCTCAG

Coding sequence (CDS)

ATGGTGACGAGATATTCCTCCATTTCCAGTAGAAAGCTAAGCAAAGCCCTCACCATTTTGACTTCTGATAAATGCATGGTTGCTATGAGTGATTACAATGACTTTCACAAGTTGGTGAAGAGATATATATTGGCTAATCTCTTGGGTGTTAATGCTCAG

Protein sequence

MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ

Homology

[Close]

BLAST of Spg023860 vs. NCBI nr
Match: KAG6608504.1 (Ent-kaurene oxidase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 100.1 bits (248), Expect = 5.9e-18
Identity = 50/53 (94.34%), Postives = 52/53 (98.11%), Query Frame = 0


Query: 1   MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ 54
           MVTRYSSISSRKLSKALTILT+DKCMVAMSDYNDFHKLVKRYILAN+LG NAQ
Sbjct: 110 MVTRYSSISSRKLSKALTILTADKCMVAMSDYNDFHKLVKRYILANVLGANAQ 162

[Close]

BLAST of Spg023860 vs. NCBI nr
Match: KAG7037828.1 (Ent-kaurene oxidase, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 100.1 bits (248), Expect = 5.9e-18
Identity = 50/53 (94.34%), Postives = 52/53 (98.11%), Query Frame = 0


Query: 1   MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ 54
           MVTRYSSISSRKLSKALTILT+DKCMVAMSDYNDFHKLVKRYILAN+LG NAQ
Sbjct: 110 MVTRYSSISSRKLSKALTILTADKCMVAMSDYNDFHKLVKRYILANVLGANAQ 162

[Close]

BLAST of Spg023860 vs. NCBI nr
Match: XP_023524792.1 (ent-kaurene oxidase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 100.1 bits (248), Expect = 5.9e-18
Identity = 50/53 (94.34%), Postives = 52/53 (98.11%), Query Frame = 0


Query: 1   MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ 54
           MVTRYSSISSRKLSKALTILT+DKCMVAMSDYNDFHKLVKRYILAN+LG NAQ
Sbjct: 110 MVTRYSSISSRKLSKALTILTADKCMVAMSDYNDFHKLVKRYILANVLGANAQ 162

[Close]

BLAST of Spg023860 vs. NCBI nr
Match: XP_022941414.1 (ent-kaurene oxidase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 100.1 bits (248), Expect = 5.9e-18
Identity = 50/53 (94.34%), Postives = 52/53 (98.11%), Query Frame = 0


Query: 1   MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ 54
           MVTRYSSISSRKLSKALTILT+DKCMVAMSDYNDFHKLVKRYILAN+LG NAQ
Sbjct: 110 MVTRYSSISSRKLSKALTILTADKCMVAMSDYNDFHKLVKRYILANVLGANAQ 162

[Close]

BLAST of Spg023860 vs. NCBI nr
Match: XP_022982224.1 (ent-kaurene oxidase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 100.1 bits (248), Expect = 5.9e-18
Identity = 50/53 (94.34%), Postives = 52/53 (98.11%), Query Frame = 0


Query: 1   MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ 54
           MVTRYSSISSRKLSKALTILT+DKCMVAMSDYNDFHKLVKRYILAN+LG NAQ
Sbjct: 110 MVTRYSSISSRKLSKALTILTADKCMVAMSDYNDFHKLVKRYILANVLGANAQ 162

[Close]

BLAST of Spg023860 vs. ExPASy Swiss-Prot
Match: Q93ZB2 (Ent-kaurene oxidase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=KO PE=1 SV=2)

HSP 1 Score: 78.6 bits (192), Expect = 2.4e-14
Identity = 40/53 (75.47%), Postives = 45/53 (84.91%), Query Frame = 0


Query: 1   MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ 54
           MVTR+SSIS+RKLS ALT+LT DK MVA SDY+DFHKLVKR +L  LLG NAQ
Sbjct: 101 MVTRFSSISTRKLSNALTVLTCDKSMVATSDYDDFHKLVKRCLLNGLLGANAQ 153

[Close]

BLAST of Spg023860 vs. ExPASy Swiss-Prot
Match: Q5Z5R4 (Ent-kaurene oxidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A6 PE=1 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 7.0e-14
Identity = 36/53 (67.92%), Postives = 47/53 (88.68%), Query Frame = 0


Query: 1   MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ 54
           MV ++SSIS+RKLSKALT+LT DK MVA SDY DFHK+VKRY+++++LG +AQ
Sbjct: 98  MVAKFSSISTRKLSKALTVLTRDKSMVATSDYCDFHKMVKRYVMSSMLGTSAQ 150

[Close]

BLAST of Spg023860 vs. ExPASy Swiss-Prot
Match: C7J3A2 (Ent-kaurene oxidase-like protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A7 PE=5 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 7.0e-14
Identity = 36/53 (67.92%), Postives = 46/53 (86.79%), Query Frame = 0


Query: 1   MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ 54
           MV ++SSIS+RKLSKALT+LT DK MVA SDY DFHK+VKRY++++ LG +AQ
Sbjct: 109 MVAKFSSISTRKLSKALTVLTRDKSMVATSDYGDFHKMVKRYVMSSTLGTSAQ 161

[Close]

BLAST of Spg023860 vs. ExPASy Swiss-Prot
Match: A0A0G2RKY1 (Ent-kaurene oxidase OS=Salvia miltiorrhiza OX=226208 GN=KO PE=1 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 1.6e-13
Identity = 37/53 (69.81%), Postives = 46/53 (86.79%), Query Frame = 0


Query: 1   MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ 54
           M+T+YSSIS+RKLSKALTILTSDK +VAMSDYN+F+K  KR++L + LG  AQ
Sbjct: 105 MITKYSSISTRKLSKALTILTSDKSIVAMSDYNEFYKAAKRHLLTSTLGPTAQ 157

[Close]

BLAST of Spg023860 vs. ExPASy Swiss-Prot
Match: Q5Z5R7 (Ent-kaurene oxidase-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A19 PE=2 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 8.6e-12
Identity = 34/53 (64.15%), Postives = 44/53 (83.02%), Query Frame = 0


Query: 1   MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ 54
           MV ++SSIS+RKLSKALT+L+ DK MVA SD  DFHK+ KRYI+ ++LG +AQ
Sbjct: 97  MVAKFSSISTRKLSKALTVLSHDKSMVATSDSGDFHKMGKRYIMLSMLGTSAQ 149

[Close]

BLAST of Spg023860 vs. ExPASy TrEMBL
Match: A0A6J1J3Y4 (ent-kaurene oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111481118 PE=3 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 2.9e-18
Identity = 50/53 (94.34%), Postives = 52/53 (98.11%), Query Frame = 0


Query: 1   MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ 54
           MVTRYSSISSRKLSKALTILT+DKCMVAMSDYNDFHKLVKRYILAN+LG NAQ
Sbjct: 110 MVTRYSSISSRKLSKALTILTADKCMVAMSDYNDFHKLVKRYILANVLGANAQ 162

[Close]

BLAST of Spg023860 vs. ExPASy TrEMBL
Match: Q9FQY5 (Ent-kaurene oxidase OS=Cucurbita maxima OX=3661 GN=CYP701A1 PE=2 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 2.9e-18
Identity = 50/53 (94.34%), Postives = 52/53 (98.11%), Query Frame = 0


Query: 1   MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ 54
           MVTRYSSISSRKLSKALTILT+DKCMVAMSDYNDFHKLVKRYILAN+LG NAQ
Sbjct: 110 MVTRYSSISSRKLSKALTILTADKCMVAMSDYNDFHKLVKRYILANVLGANAQ 162

[Close]

BLAST of Spg023860 vs. ExPASy TrEMBL
Match: A0A6J1FN94 (ent-kaurene oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111446712 PE=3 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 2.9e-18
Identity = 50/53 (94.34%), Postives = 52/53 (98.11%), Query Frame = 0


Query: 1   MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ 54
           MVTRYSSISSRKLSKALTILT+DKCMVAMSDYNDFHKLVKRYILAN+LG NAQ
Sbjct: 110 MVTRYSSISSRKLSKALTILTADKCMVAMSDYNDFHKLVKRYILANVLGANAQ 162

[Close]

BLAST of Spg023860 vs. ExPASy TrEMBL
Match: A0A6J1CGM6 (ent-kaurene oxidase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111010641 PE=3 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 6.4e-18
Identity = 49/53 (92.45%), Postives = 51/53 (96.23%), Query Frame = 0


Query: 1   MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ 54
           MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHK+ KRYILAN+LG NAQ
Sbjct: 112 MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKMAKRYILANVLGANAQ 164

[Close]

BLAST of Spg023860 vs. ExPASy TrEMBL
Match: D5LXQ4 (Ent-kaurene oxidase OS=Momordica charantia OX=3673 GN=KO PE=2 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 6.4e-18
Identity = 49/53 (92.45%), Postives = 51/53 (96.23%), Query Frame = 0


Query: 1   MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ 54
           MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHK+ KRYILAN+LG NAQ
Sbjct: 112 MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKMAKRYILANVLGANAQ 164

[Close]

BLAST of Spg023860 vs. TAIR 10
Match: AT5G25900.1 (GA requiring 3 )

HSP 1 Score: 78.6 bits (192), Expect = 1.7e-15
Identity = 40/53 (75.47%), Postives = 45/53 (84.91%), Query Frame = 0


Query: 1   MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ 54
           MVTR+SSIS+RKLS ALT+LT DK MVA SDY+DFHKLVKR +L  LLG NAQ
Sbjct: 101 MVTRFSSISTRKLSNALTVLTCDKSMVATSDYDDFHKLVKRCLLNGLLGANAQ 153

The following BLAST results are available for this feature:

BLAST of Spg023860 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6608504.1	5.9e-18	94.34	Ent-kaurene oxidase, chloroplastic, partial [Cucurbita argyrosperma subsp. soror...	[more]
KAG7037828.1	5.9e-18	94.34	Ent-kaurene oxidase, chloroplastic, partial [Cucurbita argyrosperma subsp. argyr...	[more]
XP_023524792.1	5.9e-18	94.34	ent-kaurene oxidase, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]
XP_022941414.1	5.9e-18	94.34	ent-kaurene oxidase, chloroplastic-like [Cucurbita moschata]	[more]
XP_022982224.1	5.9e-18	94.34	ent-kaurene oxidase, chloroplastic-like [Cucurbita maxima]	[more]

BLAST of Spg023860 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93ZB2	2.4e-14	75.47	Ent-kaurene oxidase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=KO PE=1 SV...	[more]
Q5Z5R4	7.0e-14	67.92	Ent-kaurene oxidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A6 PE=1 ...	[more]
C7J3A2	7.0e-14	67.92	Ent-kaurene oxidase-like protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=C...	[more]
A0A0G2RKY1	1.6e-13	69.81	Ent-kaurene oxidase OS=Salvia miltiorrhiza OX=226208 GN=KO PE=1 SV=1	[more]
Q5Z5R7	8.6e-12	64.15	Ent-kaurene oxidase-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A19...	[more]

BLAST of Spg023860 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J3Y4	2.9e-18	94.34	ent-kaurene oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111481...	[more]
Q9FQY5	2.9e-18	94.34	Ent-kaurene oxidase OS=Cucurbita maxima OX=3661 GN=CYP701A1 PE=2 SV=1	[more]
A0A6J1FN94	2.9e-18	94.34	ent-kaurene oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1CGM6	6.4e-18	92.45	ent-kaurene oxidase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC11101064...	[more]
D5LXQ4	6.4e-18	92.45	Ent-kaurene oxidase OS=Momordica charantia OX=3673 GN=KO PE=2 SV=1	[more]

BLAST of Spg023860 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G25900.1	1.7e-15	75.47	GA requiring 3	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044225	Ent-kaurene oxidase, chloroplastic	PANTHER	PTHR47283	ENT-KAURENE OXIDASE, CHLOROPLASTIC	coord: 1..53

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg023860.1	Spg023860.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010241	ent-kaurene oxidation to kaurenoic acid
biological_process	GO:0009686	gibberellin biosynthetic process
cellular_component	GO:0009707	chloroplast outer membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0052615	ent-kaurene oxidase activity
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding