Spg023844 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg023844
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionbasic 7S globulin-like
Locationscaffold13: 2601114 .. 2607007 (+)
RNA-Seq ExpressionSpg023844
SyntenySpg023844
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTGGACATATCTGAGTTTGGAGTGGTTGTGGCGGACGTTTGAGGCGGTGCGGCTTCAGCTTCTGTCGGCGAACGGCGAATATGGTGGCTCACGAGGCGGCGAGAGTGGCGGTAGACGAAGGAGAAGAGGGGATCTGGTTAGAGGACTGTCCGAATGGCCTTCTTCCGACTGTCCAAAGAGATATGAGTGGGCCTGGTTTGTGGGCTTCTGATTCTATGTAACTCTGTTTGTTGATTCTTTCTTCAAAAAAAATGAATAATTGTTTTTTTTTTTTTTTTGAGAAGTGGATAATTGTGTTCCTTAATTTCGAATTTTGTTCTTTTTAGATTCAAAAAATGATACAATTTAAGTCTTAGAATATGATTGACTTATAAAGTTGTCGAATGACGTGATAAATTTTATTATCATCCTACTTTTTTTTTTTATTATAAAAGTCAAATATATTATAAATAATCAGTAATTGATAAATATAAAATATTAAATACTGGATTATTCAAAAATAACTTTTAAGTCATTAACCGAAAATCCATAAAAAAATAATAACAAATTTATAATTAATAGAATCATTTTTTAATATAGTACAAGTGCAATCAAACAAATTTGTTATTTGAAATACTTTTGAAAAAAATGTTAGACATAATATTTTTTGTAAATTTTTTTTTCCAAAAGTGTTTAAACTAAAGGTATTTCAAACAAATTCTAAGGCCCCGTTTGATAACCATTTGGTTTTTTGTTTTTGGTTTTTGAAAATTATACTTGTTTTTTCCCAATTTTCTTATCATGATTTGCATCTTTGTCAATGACACATTTAACTTCCTAGCCAAATTCTAAAAACAAAAACAAGTTTTTGGAAACTACTTTTTTTTAGTTTCCAAAATTTGGCTTGGTTTTTGAAAACATAAGAAGAAGGTAGGTAACAAAATCAAGAAACTTATGTGTAGGAGTAGTGTTTTTAAGCTTAATTTTCAAAAACCAAATGGTTATCAAACGGGGCCTAAGTAATTTGTCTAAGTAACAAATGAAAAGTAAATACTCTTTGTTTTAGTTCAACAAATATGAATTGAAGATTGAAGTTTGAACCTCAACAAACATATTATGTCTCGTGGACGAGAGAGATATGAGAAACTTTTTCTATTGTCTTTTTTAAAGTTCCTTTCATCCTCTGCATATCTTTCCAAACTAGTGCCAATCATGATAAATGGGAAAATAAAATAAATCTCTAAATTTGGTGGAAAAATTGTCAATGTCTTATATTTTCTTCCAAAAAAATCCTCAACTTCAATCGATCCACATACTCTCCATCGTTGCTTTGGGAATGAGAGTTGCACATAAACTAAAGTGGTGAAGTGGTTGGAGGTGAGTTAAAATGAAATGATGGAGAATTTGACGGCTTGGAAATCTAAGTGTTGTTCGACTTGGGGTAAGCTGTGGGTTATAATAGATGTAGGTTGAAATGACGAGAGCTGAGGTAACAATGATGTTTGAGATTAATCAAACATGATTTTGTCGAGAATTGAAGAATGATAGGGTACTTCTTTCTTTACAAGGCTCGTGGAATAGTGTATTCACTTAGATTGAAAAAAAAAACCATATACTTGATATTTAATAAAGATAGTGATCAAAGCCAAATAATAATAACAATAATAATGTAAAAGGACTTGTTACAAAGGAATATTCTTAGAAGGGAATCAAATATGCCTTTTATAGAAGTGATGTTGAATTAAGCATGATCACACATGAAAAGGCACAACACGCATTATTGGTCTGATGGTCTAGTTAGAGGTGGTCGTCTGTCGGTCAGTGTTGGTTTTAGCAGAAAACCAACAGTTTTGGAGGTTGTCTGCTGCCTACTAACCAATTGGTCGATTTGATCGATTTTCTAGTTTTTTGATACCTAATTTGTTGAGACAATGTTGGTGGTGGTGGTGTATATTTTCGAGGTAGGTAGAAAAGGAAGGAAGACAAGATGGAAAAGAGGAAAACGGTGGTCACGGCAATAAGAGACAATGGTGGCTACTCAACGGTAAGGACATACGGTAGTGGCACAGTGGCAAGGAGAGATGGAGAGAAAGAATAAGACAAGAATCATATGAGATGGGGAGGGAGGGTAGTCACAGTCGACAATCATGGTAAAAGAGACCCAAAATGTAAGATTAGGAGGAAGAAGGAGAGGGCAGAAGTAAAAATGCTTAAGGTTAAACTTGAAATTGCCACAAACGATGACATTTTGGCAAACATAGGGGCAATTTTATTTTTTATTATATAGTCGGTCGGTCAGTTGGTTTGGTCCAGATTTCATAGACCAACTATCATCCTTTTTTTTTTTTAATAAGACCGACTCCGACCGATTAAGTCATAAAAATGGTCAACTAACTTTAATTCGATCAATAGTTTCAAAAAAAAACTTTAGTTCGATCAATTTTGATTGGTCAAACTTATTTTTAGTCTAATTTTGTTCACCTCTAAGTTTAATAAAAACATTGTATACTATAGAAACAAACATATTGCAAATACAATTTGTCTAACGAAAATATAAAAACATACAAATTAGTAAAGCAAGACATTCTATGAAACCAAGCATCAAACAAAAATCATCCTAGAAACGGTTTATTATGATTCTAAAAGCCTCCAATACATAGGAGGAATCTAATATTCAGGGAGGATTCAAGCTAACAAATCAAACTAAACTAACAAACAACCCACTAAAACAAAATTACAAAATAACTCTCACATAATATACTACGTTAAGATTACTAACATCATTCCCAAGGCTAAGATTGGATTATGGAAAATCATCAAAAACCTCATACCTACTAAAGACAATTTAATCAGAAAGGGGATGGACATTAACCCTTTGTGTGATTTGTGCAGGTGCTCGAGAGAAGATGCGAGTCATATCTTTTGGGATTGCAAGCAGGTAAAGAGTAGTTGGCCAGAGCTATTCCCAAAGCTTATGAATTGTTCTGATATTTGTAGGAATGAGCTCAATGTAGCCGAGCGTTGGGAGGTGATATCAAGATTGGTCGATAAAGAAGATTTGGAGCAGATTGCGATTGCGATGTGGACAACTTGGAGCTTTAGATACAAGATTACGATCAACAATGACAAGATTGGCATCTACAAGTTAGAGATGTGGATTAAAAGAAACCTCAAGGAGCAAAGAAAACGTCTATCGAACCTGAAGGATCCCAAGTTGGAGAGCCTCATGAATCAGGAAGGTTGGACCCCCCTGAGGACTGTTTGGAGATGAATTCTGATGCATCTTGGAACGAAGACTCGAAAACTAGGGGAATTGACTGGATTGTTCGTGATTCATCAGGATCTCTAATAGAAGCGGGATGCAAGCAAATTGGGAGAAGATGACAAATTAAATCCATGGAAGTTCTAGCGATTAAAGAGGGATTGAAGGCCTACATCTCGAGGAATCAAAGAAGCAACGTACCTTTAATTGTCGAAGCGGATGCAATGGAGGTCGTCAAGTCGCTCAACTTCGAGTCCGTGGACATCTCAGATACCGAGTTGATCGTGAACGAAGTGATTTCCCTAACGTCGAAAGCAGGGGTGATTTACTTTGCCAAATGCCCGAGAAAGGGCAATCAAATAGCGCATTCTCTCGCACGTGCGGCGACAGGGTTACCGTCGGCCCCTTCCCTGCCGTCCGGTGTTGTCGGTAGTTTTTGTGATTCGTTATTTTTTGGTTCGTCTTCCATGTTGGAAGTCGATTTTGTTGGGAGGGATTCTCATATCCGAGTGTGGTTGTCCTCTTTAATTGAGGATATTGGTGTAACTACCCCCTTTTCTCATTAATGAATTTTCAGTTTAAAAAGAAAGATTACTTAAAAGAGCTAAACAGTGGTTAAATGACATTTTCACACATGGTTTATTTGCAACCATCTATGGGTATTACATTATTTTTTATGAAACAAAATGAAAAACAAACTCAAAGCAAAATTTCAGCACATATCAAATAATTAAACCGCACGCAAGTCGAACATTTCCTACCGTGCTGCTTGTTGAGTCTTGCACAACAAGATATGTGCCATTAGATATCACATTTTGATGAATAAGTGAATTTTGTTGTATCGTTAAACCGTTATAAGTAAACCTCATAAAAAAAACATAAGTAAAATATTTGACGTTAAAAAATATACCACGTCAAAAAGTAAAATCTTTGACACTAAATTCTCAAATACAACAAATATATAAGAAAAATCCAAATAAATGTATACCAAAAATACTCCCATGGTTTATGTTCTATTCTAGAATGAAGGTGTGACACATCACACACACATGACCCACAATTTCATTTGGCACATGGATTTGTTAAAAACCATTCCACCATATTTTAAATTTAAAAAAAAAATAAAAAATTGCCCAAAGAAGATAGACATAAAGATAGCTCAAATTCATTTCCACCTCAGCCTCCCATAATTTTTCTTCCTAATATCTCTTTCACTTTTTTTTTTTTTTTTTAATTTATACAAAATTTTTATAAATATATTTCCTCTAATAAACTGCTCCACTTGGCCCTTATCCAATTCCCACACACATTCCAATCTCTCTCTCTCTCTCTCTCTCTTTCTCTCTTTCTCTCTCTAACCTTATTCCCAAACCGCGGCGAACCTCCGCCGACTGTACCAAATTTCCATGGCTGTTCCCCAATACCCTTCTCTTCTTCTTCTTCTCTGTTTCTTTACCCTCTTCCATGGCGGAACCTTCTCTCTCGTCATTCCGGTCACCAAAGATCCCGTTACTGATCAGTATTTGGCCGCAGTCCACCATGGCTCTCCGATCAAGCCGACCCATCTCGCCGTCGATCTCGGCGGGCCGTCTCTCTGGATGGCCTGTGGCGGATCTTCCTCCAGCCGCCCGATTCCGAGGCGGTCGATTCAGTGCGTTGCGGCCGCGGGCGGTGGTGGTGGATCTGGGTCTGGAGGCGGCGGCGCGTGTGATGTAGTTGCTGGGAATCCGTTTGGGGCTTTGGTAGGGAAGGCCGTGCTGGTTGAGGATACGGTGGCGGTGAGGTCTTTCGACCGGTCAACGGCGGCGGTGATTGTTGCAGTTCATTCTTGTGCTCCGAGGTTTCTTTTGCAGGGTTTGGCTAAATCTGCCAGGGGGATTTTAGGGCTTGGCCGGAATCAGAACTCGCTTCCGGCTCAGATCGCGACGGAGCTCGGTTTTCACCGGCGGTTTTCTGTTTGTCTCTCGTCGACGAACGGCGTCGTCTTTCCGGACAGTGGAAGTCAAGACTCTGTTTATGGATCTGAGATTTCGAGTTCTTTGACTTACACGCCGATTTTGACTAAGAAAATCGACGGCTCGCAGTCGCCGGAGTATTTCATCAACGTTAAGGCCATCAAAGTCGACGGTAACCGTCTCGATCTGAACAAATCGTTACTCGATTTGGACGGCGGCGAAGGCGTCGGAGGGACAAGACTGAGCACGGTGGTTCCGTACACCGCACTAGAGAGTTCGATCTTTAAATCTCTGACGGCAGCGTTCAGGGCGGCCGCGGCGGCGATGAACATAAAAGAAGTGGCTCCGGTGGCGCCGTTCGAGGCGTGTTTTGCGGCGGAGAACATGGAGATGACGGCGACGGGGCCGAAAGTGCCGGAGATTGAACTGATTTTGCAGAGCGAGATGGTGGGATGGAAAATCGCCGGCAGAAATTCGATGGTGAAGGTGAACGATGAAACTTTCTGTTTAGGGTTTGTGGACGGCGGATTGAAGCCGAAAAACGCCGTCGTTTTGGGAGGTTATCAAATGGAAGATATCGTCCTTGACTTCGACATGGGAACTTCGATGCTCGGATTCAGCTCGTCTTTGTTGCAGAGGAAGAGATCTTGCTCGGAATTCTCGCCGGAAAATTTACTGAAAAAAACAGGATGA

mRNA sequence

ATGGAGTGGACATATCTGAGTTTGGAGTGGTTGTGGCGGACGTTTGAGGCGGTGCGGCTTCAGCTTCTGTCGGCGAACGGCGAATATGGTGGCTCACGAGGCGGCGAGAGTGGCGGTAGACGAAGGAGAAGAGGGGATCTGGTTAGAGGACTGTCCGAATGGCCTTCTTCCGACTGTCCAAAGAGATATGAGTGGGCCTGGTTTGTAGAAAAGGAAGGAAGACAAGATGGAAAAGAGGAAAACGGTGGTCACGGCAATAAGAGACAATGGTGGCTACTCAACGGTAAGGACATACGGTGCTCGAGAGAAGATGCGAGTCATATCTTTTGGGATTGCAAGCAGGTAAAGAGTAGTTGGCCAGAGCTATTCCCAAAGCTTATGAATTGTTCTGATATTTGTAGGAATGAGCTCAATGTAGCCGAGCGTTGGGAGGTGATATCAAGATTGGTCGATAAAGAAGATTTGGAGCAGATTGCGATTGCGATGTGGACAACTTGGAGCTTTAGATACAAGATTACGATCAACAATGACAAGATTGGCATCTACAAGTTAGAGATGTGGATTAAAAGAAACCTCAAGGAGCAAAGAAAACGTCTATCGAACCTGAAGGATCCCAAGTTGGAGAGCCTCATGAATCAGGAAGCGGATGCAATGGAGGTCGTCAAGTCGCTCAACTTCGAGTCCGTGGACATCTCAGATACCGAGTTGATCGTGAACGAAGTGATTTCCCTAACGTCGAAAGCAGGGGTGATTTACTTTGCCAAATGCCCGAGAAAGGGCAATCAAATAGCGCATTCTCTCGCACGTGCGGCGACAGGGTTACCGTCGGCCCCTTCCCTGCCGTCCGGTGTTGTCGATCCCGTTACTGATCAGTATTTGGCCGCAGTCCACCATGGCTCTCCGATCAAGCCGACCCATCTCGCCGTCGATCTCGGCGGGCCGTCTCTCTGGATGGCCTGTGGCGGATCTTCCTCCAGCCGCCCGATTCCGAGGCGGTCGATTCAGTGCGTTGCGGCCGCGGGCGGTGGTGGTGGATCTGGGTCTGGAGGCGGCGGCGCGTGTGATGTAGTTGCTGGGAATCCGTTTGGGGCTTTGGTAGGGAAGGCCGTGCTGGTTGAGGATACGGTGGCGGTGAGGTCTTTCGACCGGTCAACGGCGGCGGTGATTGTTGCAGTTCATTCTTGTGCTCCGAGGTTTCTTTTGCAGGGTTTGGCTAAATCTGCCAGGGGGATTTTAGGGCTTGGCCGGAATCAGAACTCGCTTCCGGCTCAGATCGCGACGGAGCTCGGTTTTCACCGGCGGTTTTCTGTTTGTCTCTCGTCGACGAACGGCGTCGTCTTTCCGGACAGTGGAAGTCAAGACTCTGTTTATGGATCTGAGATTTCGAGTTCTTTGACTTACACGCCGATTTTGACTAAGAAAATCGACGGCTCGCAGTCGCCGGAGTATTTCATCAACGTTAAGGCCATCAAAGTCGACGGTAACCGTCTCGATCTGAACAAATCGTTACTCGATTTGGACGGCGGCGAAGGCGTCGGAGGGACAAGACTGAGCACGGTGGTTCCGTACACCGCACTAGAGAGTTCGATCTTTAAATCTCTGACGGCAGCGTTCAGGGCGGCCGCGGCGGCGATGAACATAAAAGAAGTGGCTCCGGTGGCGCCGTTCGAGGCGTGTTTTGCGGCGGAGAACATGGAGATGACGGCGACGGGGCCGAAAGTGCCGGAGATTGAACTGATTTTGCAGAGCGAGATGGTGGGATGGAAAATCGCCGGCAGAAATTCGATGGTGAAGGTGAACGATGAAACTTTCTGTTTAGGGTTTGTGGACGGCGGATTGAAGCCGAAAAACGCCGTCGTTTTGGGAGGTTATCAAATGGAAGATATCGTCCTTGACTTCGACATGGGAACTTCGATGCTCGGATTCAGCTCGTCTTTGTTGCAGAGGAAGAGATCTTGCTCGGAATTCTCGCCGGAAAATTTACTGAAAAAAACAGGATGA

Coding sequence (CDS)

ATGGAGTGGACATATCTGAGTTTGGAGTGGTTGTGGCGGACGTTTGAGGCGGTGCGGCTTCAGCTTCTGTCGGCGAACGGCGAATATGGTGGCTCACGAGGCGGCGAGAGTGGCGGTAGACGAAGGAGAAGAGGGGATCTGGTTAGAGGACTGTCCGAATGGCCTTCTTCCGACTGTCCAAAGAGATATGAGTGGGCCTGGTTTGTAGAAAAGGAAGGAAGACAAGATGGAAAAGAGGAAAACGGTGGTCACGGCAATAAGAGACAATGGTGGCTACTCAACGGTAAGGACATACGGTGCTCGAGAGAAGATGCGAGTCATATCTTTTGGGATTGCAAGCAGGTAAAGAGTAGTTGGCCAGAGCTATTCCCAAAGCTTATGAATTGTTCTGATATTTGTAGGAATGAGCTCAATGTAGCCGAGCGTTGGGAGGTGATATCAAGATTGGTCGATAAAGAAGATTTGGAGCAGATTGCGATTGCGATGTGGACAACTTGGAGCTTTAGATACAAGATTACGATCAACAATGACAAGATTGGCATCTACAAGTTAGAGATGTGGATTAAAAGAAACCTCAAGGAGCAAAGAAAACGTCTATCGAACCTGAAGGATCCCAAGTTGGAGAGCCTCATGAATCAGGAAGCGGATGCAATGGAGGTCGTCAAGTCGCTCAACTTCGAGTCCGTGGACATCTCAGATACCGAGTTGATCGTGAACGAAGTGATTTCCCTAACGTCGAAAGCAGGGGTGATTTACTTTGCCAAATGCCCGAGAAAGGGCAATCAAATAGCGCATTCTCTCGCACGTGCGGCGACAGGGTTACCGTCGGCCCCTTCCCTGCCGTCCGGTGTTGTCGATCCCGTTACTGATCAGTATTTGGCCGCAGTCCACCATGGCTCTCCGATCAAGCCGACCCATCTCGCCGTCGATCTCGGCGGGCCGTCTCTCTGGATGGCCTGTGGCGGATCTTCCTCCAGCCGCCCGATTCCGAGGCGGTCGATTCAGTGCGTTGCGGCCGCGGGCGGTGGTGGTGGATCTGGGTCTGGAGGCGGCGGCGCGTGTGATGTAGTTGCTGGGAATCCGTTTGGGGCTTTGGTAGGGAAGGCCGTGCTGGTTGAGGATACGGTGGCGGTGAGGTCTTTCGACCGGTCAACGGCGGCGGTGATTGTTGCAGTTCATTCTTGTGCTCCGAGGTTTCTTTTGCAGGGTTTGGCTAAATCTGCCAGGGGGATTTTAGGGCTTGGCCGGAATCAGAACTCGCTTCCGGCTCAGATCGCGACGGAGCTCGGTTTTCACCGGCGGTTTTCTGTTTGTCTCTCGTCGACGAACGGCGTCGTCTTTCCGGACAGTGGAAGTCAAGACTCTGTTTATGGATCTGAGATTTCGAGTTCTTTGACTTACACGCCGATTTTGACTAAGAAAATCGACGGCTCGCAGTCGCCGGAGTATTTCATCAACGTTAAGGCCATCAAAGTCGACGGTAACCGTCTCGATCTGAACAAATCGTTACTCGATTTGGACGGCGGCGAAGGCGTCGGAGGGACAAGACTGAGCACGGTGGTTCCGTACACCGCACTAGAGAGTTCGATCTTTAAATCTCTGACGGCAGCGTTCAGGGCGGCCGCGGCGGCGATGAACATAAAAGAAGTGGCTCCGGTGGCGCCGTTCGAGGCGTGTTTTGCGGCGGAGAACATGGAGATGACGGCGACGGGGCCGAAAGTGCCGGAGATTGAACTGATTTTGCAGAGCGAGATGGTGGGATGGAAAATCGCCGGCAGAAATTCGATGGTGAAGGTGAACGATGAAACTTTCTGTTTAGGGTTTGTGGACGGCGGATTGAAGCCGAAAAACGCCGTCGTTTTGGGAGGTTATCAAATGGAAGATATCGTCCTTGACTTCGACATGGGAACTTCGATGCTCGGATTCAGCTCGTCTTTGTTGCAGAGGAAGAGATCTTGCTCGGAATTCTCGCCGGAAAATTTACTGAAAAAAACAGGATGA

Protein sequence

MEWTYLSLEWLWRTFEAVRLQLLSANGEYGGSRGGESGGRRRRRGDLVRGLSEWPSSDCPKRYEWAWFVEKEGRQDGKEENGGHGNKRQWWLLNGKDIRCSREDASHIFWDCKQVKSSWPELFPKLMNCSDICRNELNVAERWEVISRLVDKEDLEQIAIAMWTTWSFRYKITINNDKIGIYKLEMWIKRNLKEQRKRLSNLKDPKLESLMNQEADAMEVVKSLNFESVDISDTELIVNEVISLTSKAGVIYFAKCPRKGNQIAHSLARAATGLPSAPSLPSGVVDPVTDQYLAAVHHGSPIKPTHLAVDLGGPSLWMACGGSSSSRPIPRRSIQCVAAAGGGGGSGSGGGGACDVVAGNPFGALVGKAVLVEDTVAVRSFDRSTAAVIVAVHSCAPRFLLQGLAKSARGILGLGRNQNSLPAQIATELGFHRRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKIDGSQSPEYFINVKAIKVDGNRLDLNKSLLDLDGGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNIKEVAPVAPFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDETFCLGFVDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLKKTG
Homology
BLAST of Spg023844 vs. NCBI nr
Match: KAG7026255.1 (Basic 7S globulin, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 641.3 bits (1653), Expect = 8.9e-180
Identity = 339/380 (89.21%), Postives = 354/380 (93.16%), Query Frame = 0

Query: 286 DPVTDQYLAAVHHGSPIKPTHLAVDLGGPSLWMACGGSSSSRPIPRRSIQCVAAAGGGGG 345
           D VT+QYLA+V+HGSPIKP HLAVDLGGPSLWMACG S+SSR I  RSIQCVAA GGG  
Sbjct: 34  DSVTNQYLASVYHGSPIKPIHLAVDLGGPSLWMACGSSTSSRTIQSRSIQCVAATGGGSR 93

Query: 346 SGSGGGGACDVVAGNPFGALVGKAVLVEDTVAVRSFDRSTAAVIVAVHSCAPRFLLQGLA 405
           SGS GGGAC+VVAGNPFGALVGKA+LVEDTVAVR+ +RSTAAVIVAVHSCAPRFLLQGLA
Sbjct: 94  SGS-GGGACEVVAGNPFGALVGKALLVEDTVAVRALERSTAAVIVAVHSCAPRFLLQGLA 153

Query: 406 KSARGILGLGRNQNSLPAQIATELGFHRRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSL 465
           KSA+GILGLGRNQ SLPAQIATELGFHRRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSL
Sbjct: 154 KSAKGILGLGRNQISLPAQIATELGFHRRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSL 213

Query: 466 TYTPILTKKIDGSQSPEYFINVKAIKVDGNRLDLNKSLLDLDGGEGVGGTRLSTVVPYTA 525
           TYTPILTK IDGSQS EYFINVKAIKVDGNRLDLNKSLLDLDG +G GGTRLSTVVPYTA
Sbjct: 214 TYTPILTKIIDGSQSAEYFINVKAIKVDGNRLDLNKSLLDLDGEDG-GGTRLSTVVPYTA 273

Query: 526 LESSIFKSLTAAFRAAAAAMNIKEVAPVAPFEACFAAENMEMTATGPKVPEIELILQSEM 585
           LESSIFKSLTAAFR AA AMN+KEVAPVA FEACF++ENME+TA GPKVP IELILQSEM
Sbjct: 274 LESSIFKSLTAAFREAARAMNMKEVAPVASFEACFSSENMEITAAGPKVPMIELILQSEM 333

Query: 586 VGWKIAGRNSMVKVNDETFCLGFVDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSS 645
           V WKI GRNSMVKVNDETFCL  VDGGLKPKNAVVLGGYQMEDIVLDFDMG+SMLGFSSS
Sbjct: 334 VRWKIDGRNSMVKVNDETFCLAIVDGGLKPKNAVVLGGYQMEDIVLDFDMGSSMLGFSSS 393

Query: 646 LLQRKRSCSEFSPENLLKKT 666
           LLQRKRSCSEFSPENLLK T
Sbjct: 394 LLQRKRSCSEFSPENLLKST 411

BLAST of Spg023844 vs. NCBI nr
Match: XP_022959401.1 (basic 7S globulin-like [Cucurbita moschata])

HSP 1 Score: 640.6 bits (1651), Expect = 1.5e-179
Identity = 335/381 (87.93%), Postives = 350/381 (91.86%), Query Frame = 0

Query: 286 DPVTDQYLAAVHHGSPIKPTHLAVDLGGPSLWMACGGSSSSRPIPRRSIQCVAAAGGGGG 345
           D VT+QYLA ++HGSPIKP HLAVDLGG  LWMACGGSSSSRPI  RSIQC+AA GGG  
Sbjct: 36  DSVTNQYLATLYHGSPIKPVHLAVDLGGQFLWMACGGSSSSRPILSRSIQCIAAVGGGRS 95

Query: 346 SGSGGGGACDVVAGNPFGALVGKAVLVEDTVAVRSFDRSTAAVIVAVHSCAPRFLLQGLA 405
             S  G ACDV+AGNPFG L GKAVLVEDTVAVRS DRSTAAVIVA+HSCAPRFLLQGLA
Sbjct: 96  GSS--GSACDVIAGNPFGDLEGKAVLVEDTVAVRSLDRSTAAVIVALHSCAPRFLLQGLA 155

Query: 406 KSARGILGLGRNQNSLPAQIATELGFHRRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSL 465
           +SA+G+LGLGRNQ SLPAQIATELGFHRRFSVCLSSTNGVVFPDSG+ DSVYGSEISSSL
Sbjct: 156 ESAKGVLGLGRNQISLPAQIATELGFHRRFSVCLSSTNGVVFPDSGNLDSVYGSEISSSL 215

Query: 466 TYTPILTKKIDGSQSPEYFINVKAIKVDGNRLDLNKSLLDLDGGEGVGGTRLSTVVPYTA 525
           TYTPILTKKID SQSPEYFINVKAIKVDGNRLDLNKSLLDLDGG G GGTRLSTVVPYT 
Sbjct: 216 TYTPILTKKIDASQSPEYFINVKAIKVDGNRLDLNKSLLDLDGGSG-GGTRLSTVVPYTT 275

Query: 526 LESSIFKSLTAAFRAAAAAMNIKEVAPVAPFEACFAAENMEMTATGPKVPEIELILQSEM 585
           LESSIF SLTAAFRAAAAAMN+KEVAPVAPFEACF +ENMEMTATGPKVPEIELILQSEM
Sbjct: 276 LESSIFNSLTAAFRAAAAAMNMKEVAPVAPFEACFESENMEMTATGPKVPEIELILQSEM 335

Query: 586 VGWKIAGRNSMVKVNDETFCLGFVDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSS 645
           VGWKI GRNSMVKVNDET CLGFVDGGLKP+NAVVLGGYQMEDIVLDFDMGTSMLGFSSS
Sbjct: 336 VGWKINGRNSMVKVNDETLCLGFVDGGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSS 395

Query: 646 LLQRKRSCSEFSPENLLKKTG 667
           LLQRK+SCSEFSPENL  + G
Sbjct: 396 LLQRKKSCSEFSPENLPIRAG 413

BLAST of Spg023844 vs. NCBI nr
Match: KAG7013583.1 (Basic 7S globulin, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 639.0 bits (1647), Expect = 4.4e-179
Identity = 336/384 (87.50%), Postives = 350/384 (91.15%), Query Frame = 0

Query: 286 DPVTDQYLAAVHHGSPIKPTHLAVDLGGPSLWMACGGSSSSRPIPRRSIQCVAAAGGGGG 345
           D VT+QYLA ++HGSPIKP HLAVDLGG  LWMACGGSSSSRPI  RSIQC+AA GGG  
Sbjct: 35  DSVTNQYLATLYHGSPIKPVHLAVDLGGRFLWMACGGSSSSRPILSRSIQCIAAVGGGRS 94

Query: 346 SGSGGGGACDVVAGNPFGALVGKAVLVEDTVAVRSFDRSTAAVIVAVHSCAPRFLLQGLA 405
             S  G ACDV+AGNPFG L GKAVLVEDTVAVRS DRSTAAVIVA+HSCAPRFLLQGLA
Sbjct: 95  GSS--GSACDVIAGNPFGDLEGKAVLVEDTVAVRSLDRSTAAVIVALHSCAPRFLLQGLA 154

Query: 406 KSARGILGLGRNQNSLPAQIATELGFHRRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSL 465
           KSA+G+LGLGRNQ SLPAQIATELGFHRRFSVCLSSTNGVVFPDSG+ DSVYGSEISSSL
Sbjct: 155 KSAKGVLGLGRNQISLPAQIATELGFHRRFSVCLSSTNGVVFPDSGNLDSVYGSEISSSL 214

Query: 466 TYTPILTKKIDGSQSPEYFINVKAIKVDGNRLDLNKSLLDLD---GGEGVGGTRLSTVVP 525
           TYTPILTKKID SQSPEYFINVKAIKVDGNRLDLNKSLLDLD   GG G GGTRLSTVVP
Sbjct: 215 TYTPILTKKIDASQSPEYFINVKAIKVDGNRLDLNKSLLDLDGRSGGGGGGGTRLSTVVP 274

Query: 526 YTALESSIFKSLTAAFRAAAAAMNIKEVAPVAPFEACFAAENMEMTATGPKVPEIELILQ 585
           YT LESSIF SLTAAFRAAAAAMN+KEVAPVAPFEACF +ENMEMTATGPKVPEIELILQ
Sbjct: 275 YTTLESSIFNSLTAAFRAAAAAMNMKEVAPVAPFEACFESENMEMTATGPKVPEIELILQ 334

Query: 586 SEMVGWKIAGRNSMVKVNDETFCLGFVDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGF 645
           SEMVGWKI GRNSMVKVNDET CLGFVDGGLKP+NAVVLGGYQMEDIVLDFDMGTSMLGF
Sbjct: 335 SEMVGWKINGRNSMVKVNDETLCLGFVDGGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGF 394

Query: 646 SSSLLQRKRSCSEFSPENLLKKTG 667
           SSSLLQRK+SCSEFSPENL  + G
Sbjct: 395 SSSLLQRKKSCSEFSPENLPIRAG 416

BLAST of Spg023844 vs. NCBI nr
Match: XP_022930452.1 (basic 7S globulin-like [Cucurbita moschata])

HSP 1 Score: 638.3 bits (1645), Expect = 7.6e-179
Identity = 338/380 (88.95%), Postives = 352/380 (92.63%), Query Frame = 0

Query: 286 DPVTDQYLAAVHHGSPIKPTHLAVDLGGPSLWMACGGSSSSRPIPRRSIQCVAAAGGGGG 345
           D VT+QYLA+V+HGSPIKP HL VDLGGPSLWMACG S+SSR I  RSIQCVAA GGG  
Sbjct: 33  DSVTNQYLASVYHGSPIKPIHLVVDLGGPSLWMACGSSTSSRTIQSRSIQCVAATGGGSR 92

Query: 346 SGSGGGGACDVVAGNPFGALVGKAVLVEDTVAVRSFDRSTAAVIVAVHSCAPRFLLQGLA 405
           SGS GGGAC+VVAGNPFGALVGKA+LVEDTVAVR+ +RSTAAVIVAVHSCAPRFLLQGLA
Sbjct: 93  SGS-GGGACEVVAGNPFGALVGKALLVEDTVAVRALERSTAAVIVAVHSCAPRFLLQGLA 152

Query: 406 KSARGILGLGRNQNSLPAQIATELGFHRRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSL 465
           KSA+GILGLGRNQ SLPAQIATELGFHRRFSVCLSSTNGVVFPDS SQDSVYGSEISSSL
Sbjct: 153 KSAKGILGLGRNQISLPAQIATELGFHRRFSVCLSSTNGVVFPDSRSQDSVYGSEISSSL 212

Query: 466 TYTPILTKKIDGSQSPEYFINVKAIKVDGNRLDLNKSLLDLDGGEGVGGTRLSTVVPYTA 525
           TYTPILTK IDGSQS EYFINVKAIKVDGNRLDLNKSLLDLDG +G GGTRLSTVVPYTA
Sbjct: 213 TYTPILTKIIDGSQSAEYFINVKAIKVDGNRLDLNKSLLDLDGEDG-GGTRLSTVVPYTA 272

Query: 526 LESSIFKSLTAAFRAAAAAMNIKEVAPVAPFEACFAAENMEMTATGPKVPEIELILQSEM 585
           LESSIFKSLTAAFR AA AMN+KEVAPVA FEACF++ENMEMTA GPKVP IELILQSEM
Sbjct: 273 LESSIFKSLTAAFREAARAMNMKEVAPVASFEACFSSENMEMTAAGPKVPMIELILQSEM 332

Query: 586 VGWKIAGRNSMVKVNDETFCLGFVDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSS 645
           V WKI GRNSMVKVNDETFCL  VDGGLKPKNAVVLGGYQMEDIVLDFDMG+SMLGFSSS
Sbjct: 333 VRWKIDGRNSMVKVNDETFCLAIVDGGLKPKNAVVLGGYQMEDIVLDFDMGSSMLGFSSS 392

Query: 646 LLQRKRSCSEFSPENLLKKT 666
           LLQRKRSCSEFSPENLLK T
Sbjct: 393 LLQRKRSCSEFSPENLLKST 410

BLAST of Spg023844 vs. NCBI nr
Match: KAG6593913.1 (putative aspartic proteinase GIP2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 638.3 bits (1645), Expect = 7.6e-179
Identity = 337/380 (88.68%), Postives = 353/380 (92.89%), Query Frame = 0

Query: 286 DPVTDQYLAAVHHGSPIKPTHLAVDLGGPSLWMACGGSSSSRPIPRRSIQCVAAAGGGGG 345
           D VT+QYLA+V+HGSPI+P HLAVDLGGPSLWMACG S+SSR I  RSIQCVAA GGG  
Sbjct: 34  DSVTNQYLASVYHGSPIRPIHLAVDLGGPSLWMACGSSTSSRTIQSRSIQCVAATGGGSR 93

Query: 346 SGSGGGGACDVVAGNPFGALVGKAVLVEDTVAVRSFDRSTAAVIVAVHSCAPRFLLQGLA 405
           SGS GGGAC+VVAGNPFGALVGKA+LVEDTVAVR+ +RS AAVIVAVHSCAPRFLLQGLA
Sbjct: 94  SGS-GGGACEVVAGNPFGALVGKALLVEDTVAVRALERSAAAVIVAVHSCAPRFLLQGLA 153

Query: 406 KSARGILGLGRNQNSLPAQIATELGFHRRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSL 465
           KSA+GILGLGRNQ SLPAQIATELGFHRRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSL
Sbjct: 154 KSAKGILGLGRNQISLPAQIATELGFHRRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSL 213

Query: 466 TYTPILTKKIDGSQSPEYFINVKAIKVDGNRLDLNKSLLDLDGGEGVGGTRLSTVVPYTA 525
           TYTPILTK IDGSQS EYFINVKAIKVDGNRLDLNKSLLDLDG +G GGTRLSTVVPYTA
Sbjct: 214 TYTPILTKIIDGSQSAEYFINVKAIKVDGNRLDLNKSLLDLDGEDG-GGTRLSTVVPYTA 273

Query: 526 LESSIFKSLTAAFRAAAAAMNIKEVAPVAPFEACFAAENMEMTATGPKVPEIELILQSEM 585
           LESSIFKSLTAAFR AA AMN+KEVAPVA FEACF++ENME+TA GPKVP IELILQSEM
Sbjct: 274 LESSIFKSLTAAFREAARAMNMKEVAPVASFEACFSSENMEITAAGPKVPMIELILQSEM 333

Query: 586 VGWKIAGRNSMVKVNDETFCLGFVDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSS 645
           V WKI GRNSMVKVNDETFCL  VDGGLKPKNAVVLGGYQMEDIVLDFDMG+SMLGFSSS
Sbjct: 334 VRWKIDGRNSMVKVNDETFCLAIVDGGLKPKNAVVLGGYQMEDIVLDFDMGSSMLGFSSS 393

Query: 646 LLQRKRSCSEFSPENLLKKT 666
           LLQRKRSCSEFSPENLLK T
Sbjct: 394 LLQRKRSCSEFSPENLLKST 411

BLAST of Spg023844 vs. ExPASy Swiss-Prot
Match: P0DO21 (Probable aspartic proteinase GIP2 OS=Nicotiana benthamiana OX=4100 GN=GIP2 PE=1 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 6.2e-67
Identity = 161/408 (39.46%), Postives = 232/408 (56.86%), Query Frame = 0

Query: 281 PSGVVDPVTD-----QYLAAVHHGSPIKPTHLAVDLGGPSLWMACGG---SSSSRPIPRR 340
           P G++ P+T      QYL  +   +P+ P  L +DLGG  LW+ C     SS+ RP   R
Sbjct: 31  PKGLILPITKDALTLQYLTQIQQRTPLVPVSLTLDLGGQFLWVDCDQGYVSSTYRPARCR 90

Query: 341 SIQCVAAAGGGGGSG--------SGGGGACDVVAGNPFGALVGKAVLVEDTVAVRSFDRS 400
           S QC + AG G G G              C ++  N          L  DTV V+S +  
Sbjct: 91  SAQC-SLAGAGSGCGQCFSPPKPGCNNNTCGLLPDNTITRTATSGELASDTVQVQSSNGK 150

Query: 401 TAAVIVA----VHSCAPRFLLQGLAKSARGILGLGRNQNSLPAQIATELGFHRRFSVCLS 460
                V+    +  C   FLL+GLA   +G+ GLGR + SLP+Q + E  F R+F+VCLS
Sbjct: 151 NPGRHVSDKDFLFVCGSTFLLEGLASGVKGMAGLGRTRISLPSQFSAEFSFPRKFAVCLS 210

Query: 461 S---TNGVVFPDSGSQDSVYGSEISSS-LTYTPILTKKIDGSQ-------SPEYFINVKA 520
           S   + GVV    G    +   E +++  +YTP+    +  +        S EYFI VK+
Sbjct: 211 SSTNSKGVVLFGDGPYTFLPNREFANNDFSYTPLFINPVSTASAFSSREPSSEYFIGVKS 270

Query: 521 IKVDGNRLDLNKSLLDLDGGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNIKE 580
           IK++   + +N +LL +D  +GVGGT++STV PYT LE+SI+ ++T  F      +NI  
Sbjct: 271 IKINEKVVPINTTLLSID-NQGVGGTKISTVNPYTILETSIYNAVTNFF--VKELVNITR 330

Query: 581 VAPVAPFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDETFCLGFV 640
           VA VAPF ACF + N+  T  GP VP I+L+LQ+E V W+I G NSMV+V++   CLGFV
Sbjct: 331 VASVAPFRACFDSRNIASTRVGPAVPSIDLVLQNENVFWRIFGANSMVQVSENVLCLGFV 390

Query: 641 DGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFS 658
           DGG+ P+ ++V+GGY +ED +L FD+  S LGF+SS+L R+ +C+ F+
Sbjct: 391 DGGVSPRTSIVVGGYTIEDNLLQFDLARSRLGFTSSILFRQTTCANFN 434

BLAST of Spg023844 vs. ExPASy Swiss-Prot
Match: I1JNS6 (Probable aspartic proteinase GIP1 OS=Glycine max OX=3847 GN=GIP1 PE=1 SV=2)

HSP 1 Score: 218.0 bits (554), Expect = 3.2e-55
Identity = 143/395 (36.20%), Postives = 221/395 (55.95%), Query Frame = 0

Query: 286 DPVTDQYLAAVHHGSPIKPTHLAVDLGGPSLWMACGG---SSSSRPIPRRSIQCVAAAGG 345
           D  T  Y  +V   +P++PT L + LG    W+ C     SSSS  IP  +  C +    
Sbjct: 35  DDTTQLYTLSVFLKTPLQPTKLHLHLGSSLSWVLCDSTYTSSSSHHIPCNTPLCNSFP-- 94

Query: 346 GGGSGSGGGGACDVVAGNPF--GALVGKAVLVEDTVAVRSFDRSTAAVIVA--VHSCAPR 405
              + S     C +   NP     L+  A++  D++A+ ++D S++ V+++  + SCA  
Sbjct: 95  -SNACSNNSSLCALFPENPVTRNTLLDTALI--DSLALPTYDASSSLVLISDFIFSCATA 154

Query: 406 FLLQGLAKSARGILGLGRNQNSLPAQIATELGFHRRFSVCL---SSTNGVVFPDSGSQDS 465
            LLQGLA +A G+  LGR+  SLPAQI+T L   R F++CL   S+  G     S +   
Sbjct: 155 HLLQGLAANALGLASLGRSNYSLPAQISTSLTSPRSFTLCLPASSANTGAAIFASTASSF 214

Query: 466 VYGSEISSSLTYTPILTKKI------DGSQ-SPEYFINVKAIKVDGNRLDLNKSLLDLDG 525
           ++ S+I   LTYT ++   +      D  Q S EYFIN+ +IK++G  L +N S+L +D 
Sbjct: 215 LFSSKI--DLTYTQLIVNPVADTVVTDNPQPSDEYFINLTSIKINGKPLYINSSILTVD- 274

Query: 526 GEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNIKEVAPVAPFEACFAAENMEMT 585
             G GGT++ST  PYT LE+SI++     F   ++A N+     V PF  C+ A ++  T
Sbjct: 275 QTGFGGTKISTAEPYTVLETSIYRLFVQRFVNESSAFNLTVTEAVEPFGVCYPAGDLTET 334

Query: 586 ATGPKVPEIELILQSEMVGWKIAGRNSMVKV---NDETFCLGFVDGGLKPKNAVVLGGYQ 645
             GP VP ++L++ SE V W+I G NSMV+V     + +CLGFVDGG + +  +V+GG+Q
Sbjct: 335 RVGPAVPTVDLVMHSEDVFWRIFGGNSMVRVAKGGVDVWCLGFVDGGTRGRTPIVIGGHQ 394

Query: 646 MEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPEN 661
           +ED ++ FD+ ++  GF+S+LL +   CS     N
Sbjct: 395 LEDNLMQFDLDSNRFGFTSTLLLQDAKCSNLKVNN 421

BLAST of Spg023844 vs. ExPASy Swiss-Prot
Match: P82952 (Gamma conglutin 1 OS=Prunus dulcis OX=3755 GN=Cgamma1 PE=1 SV=2)

HSP 1 Score: 190.7 bits (483), Expect = 5.5e-47
Identity = 134/391 (34.27%), Postives = 201/391 (51.41%), Query Frame = 0

Query: 286 DPVTDQYLAAVHHGSPIKPTHLAVDLGGPSLWMACGG---SSSSRPIPRRSIQCVAAAGG 345
           D  T+ ++  +H  +P+      +DL G  L + C     SS+ +     S QC  A   
Sbjct: 45  DRATNLHVVQIHKRTPLVQFPFVIDLTGRFLSVNCENQYTSSTYKAPVCHSSQCARANSH 104

Query: 346 GGGSGSGG-------GGACDVVAGNPFGALVGKAVLVEDTVAVRSFDRSTAAVIVA---- 405
              + S           AC ++  NP      +  L ED + + S   S+   +V     
Sbjct: 105 TCRTCSSSKTRPGCHTNACGLLTTNPVTQQSAQGELAEDVLKIPSTQGSSPGPMVTYPHF 164

Query: 406 VHSCAPRFLLQ-GLAKSARGILGLGRNQNSLPAQIATELGFHRRFSVCLSST---NGVVF 465
           + +CAP  +LQ GL K+ +G+ GLG +  SLP Q+A+  GF  +F+VCL+S+   NG VF
Sbjct: 165 LFACAPSNILQKGLPKNVQGVAGLGHSPISLPYQLASHFGFPPKFAVCLTSSPGKNGAVF 224

Query: 466 PDSGSQDSVYGSEISSSLTYTPILTKKIDGSQSPEYFINVKAIKVDGNRLDLNKSLLDLD 525
              G      G ++S  LTY P         Q  EY+INV++ K+       N ++L   
Sbjct: 225 FGEGPYFMKPGIDVSRQLTYAPFTI-----GQQGEYYINVQSFKI-------NNAMLPSI 284

Query: 526 GGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNIKEVAPVAPFEACFAAENMEM 585
              G GG  +ST  PYT L++ IF++L   F        +  V PVAPF ACF A  +  
Sbjct: 285 PKGGFGGAMISTTTPYTTLQTPIFRALNQLF--MNQLRGVPHVKPVAPFGACFDANRIPT 344

Query: 586 TATGPKVPEIELILQSEM-VGWKIAGRNSMVKVNDETFCLGFVDGGLKPKNAVVLGGYQM 645
           +  GP VP I+L+L ++  + W+I G N+M++      CL FVDGG++PK  +V+G  Q+
Sbjct: 345 SKMGPTVPSIDLVLDNKKNIMWRIFGANAMIQPRPGVMCLAFVDGGMRPKAPIVIGTQQL 404

Query: 646 EDIVLDFDMGTSMLGFSSSLLQRKRSCSEFS 658
           ED +L FD+  S LGFSSSLL R+ +C+ F+
Sbjct: 405 EDNLLQFDLMNSRLGFSSSLLFRRTNCANFN 421

BLAST of Spg023844 vs. ExPASy Swiss-Prot
Match: Q9FSH9 (Gamma conglutin 1 OS=Lupinus albus OX=3870 GN=Cgamma PE=1 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 7.4e-36
Identity = 127/418 (30.38%), Postives = 197/418 (47.13%), Query Frame = 0

Query: 275 PSAPSLPSGVVDPVTDQYLAAVHHGSPIKPTHL-----AVDLGGPSLWMACGG--SSSSR 334
           P++ S P+ +V P+       +H G+ +K T L      +DL G  LW+ C    SSS+ 
Sbjct: 39  PTSSSKPNLLVLPIQQDASTKLHWGNILKRTPLMQVPVLLDLNGKHLWVTCSQHYSSSTY 98

Query: 335 PIP---------RRSIQCVAAAGGGGGSGSGGGGACDVVAGNPFGALVGKAVLVEDTVAV 394
             P           + QC                 C +++ NP     G   L +D +A+
Sbjct: 99  QAPFCHSTQCSRANTHQCFTCTDSTTSRPGCHNNTCGLISSNPVTQESGLGELAQDVLAL 158

Query: 395 RSFDRSTAAVIVAV----HSCAPRFLLQ-GLAKSARGILGLGRNQNSLPAQIATELGFHR 454
            S   S    +V +     SCAP FL Q GL  + +G LGLG    SLP Q+ +  G  R
Sbjct: 159 HSTHGSKLGSLVKIPQFLFSCAPTFLTQKGLPNNVQGALGLGHAPISLPNQLFSHFGLKR 218

Query: 455 RFSVCLSS---TNGVV----FPDSGSQDSVYGS-EISSSLTYTPILTKKIDGSQSPEYFI 514
           +F++CLSS   +NG +      D  + + ++ S ++   + YTP+       S+  EYFI
Sbjct: 219 QFTMCLSSYPTSNGAILFGDINDPNNNNYIHNSLDVLHDMVYTPLTI-----SKQGEYFI 278

Query: 515 NVKAIKVDGNRL--DLNKSLLDLDGGEG------VGGTRLSTVVPYTALESSIFKSLTAA 574
            V AI+V+ + +    N S+              +GG  ++T  PYT L  SIF+  T  
Sbjct: 279 QVSAIRVNKHMVIPTKNPSMFPSSSSSSYHESSEIGGAMITTTNPYTVLRHSIFEVFTQV 338

Query: 575 FRAAAAAMNIKEVAPVAPFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSMV 634
           F  A       +V  V PF  C+  + +        VP ++LI+    V W+I+G N MV
Sbjct: 339 F--ANNVPKQAQVKAVGPFGLCYDTKKI-----SGGVPSVDLIMDKSDVVWRISGENLMV 398

Query: 635 KVNDETFCLGFVDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGF-SSSLLQRKRSCS 655
           +  D   CLGFVDGG+  +  + LG +Q+E+ ++ FD+  S +GF ++SL    +SCS
Sbjct: 399 QAQDGVSCLGFVDGGVHTRAGIALGTHQLEENLVVFDLARSRVGFNTNSLKSHGKSCS 444

BLAST of Spg023844 vs. ExPASy Swiss-Prot
Match: Q42369 (Gamma conglutin 1 OS=Lupinus angustifolius OX=3871 GN=LOC109345795 PE=1 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 4.1e-34
Identity = 123/400 (30.75%), Postives = 187/400 (46.75%), Query Frame = 0

Query: 286 DPVTDQYLAAVHHGSPIKPTHLAVDLGGPSLWMACGG--SSSSRPIP---------RRSI 345
           D  T  + A +H  +P+    L +DL G  LW+ C    SSS+   P           + 
Sbjct: 54  DASTGLHWANIHKRTPLMQVPLLLDLNGKHLWVTCSQHYSSSTYQAPFCHSTQCSRANTH 113

Query: 346 QCVAAAGGGGGSGSGGGGACDVVAGNPFGALVGKAVLVEDTVAVRSFDRSTAAVIVAV-- 405
           QC                 C +++ NP     G   L +D +A+ S   S    +V V  
Sbjct: 114 QCFTCTDSTTTRPGCHNNTCGLLSSNPVTQESGLGELAQDVLAIHSTHGSKLGPMVKVPQ 173

Query: 406 --HSCAPRFLLQ-GLAKSARGILGLGRNQNSLPAQIATELGFHRRFSVCL---SSTNGVV 465
              SCAP FL Q GL  + +G LGLG+   SL  Q+ +  G  R+FSVCL   S++NG +
Sbjct: 174 FLFSCAPSFLAQKGLPNNVQGALGLGQAPISLQNQLFSHFGLKRQFSVCLSRYSTSNGAI 233

Query: 466 ----FPDSGSQDSVYGS-EISSSLTYTPILTKKIDGSQSPEYFINVKAIKVDGNRL---- 525
                 D  + + ++ S ++   L YTP+       S+  EYFI V AI+V+ + +    
Sbjct: 234 LFGDINDPNNNNYIHNSLDVLHDLVYTPLTI-----SKQGEYFIQVNAIRVNKHLVIPTK 293

Query: 526 --DLNKSLLDLDGGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNIKEVAPVAP 585
              ++ S     G   +GG  ++T  PYT L  SIF+  T  F  A       +V  V P
Sbjct: 294 NPFISPSSTSYHGSGEIGGALITTTHPYTVLSHSIFEVFTQVF--ANNMPKQAQVKAVGP 353

Query: 586 FEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDETFCLGFVDGGLKP 645
           F  C+ +  +   A     P ++LIL      W+I+  N MV+  D   CLGFVDGG+  
Sbjct: 354 FGLCYDSRKISGGA-----PSVDLILDKNDAVWRISSENFMVQAQDGVSCLGFVDGGVHA 413

Query: 646 KNAVVLGGYQMEDIVLDFDMGTSMLGF-SSSLLQRKRSCS 655
           +  + LG + +E+ ++ FD+  S +GF S+SL    ++CS
Sbjct: 414 RAGIALGAHHLEENLVVFDLERSRVGFNSNSLKSYGKTCS 441

BLAST of Spg023844 vs. ExPASy TrEMBL
Match: A0A6J1H4R8 (basic 7S globulin-like OS=Cucurbita moschata OX=3662 GN=LOC111460386 PE=4 SV=1)

HSP 1 Score: 640.6 bits (1651), Expect = 7.4e-180
Identity = 335/381 (87.93%), Postives = 350/381 (91.86%), Query Frame = 0

Query: 286 DPVTDQYLAAVHHGSPIKPTHLAVDLGGPSLWMACGGSSSSRPIPRRSIQCVAAAGGGGG 345
           D VT+QYLA ++HGSPIKP HLAVDLGG  LWMACGGSSSSRPI  RSIQC+AA GGG  
Sbjct: 36  DSVTNQYLATLYHGSPIKPVHLAVDLGGQFLWMACGGSSSSRPILSRSIQCIAAVGGGRS 95

Query: 346 SGSGGGGACDVVAGNPFGALVGKAVLVEDTVAVRSFDRSTAAVIVAVHSCAPRFLLQGLA 405
             S  G ACDV+AGNPFG L GKAVLVEDTVAVRS DRSTAAVIVA+HSCAPRFLLQGLA
Sbjct: 96  GSS--GSACDVIAGNPFGDLEGKAVLVEDTVAVRSLDRSTAAVIVALHSCAPRFLLQGLA 155

Query: 406 KSARGILGLGRNQNSLPAQIATELGFHRRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSL 465
           +SA+G+LGLGRNQ SLPAQIATELGFHRRFSVCLSSTNGVVFPDSG+ DSVYGSEISSSL
Sbjct: 156 ESAKGVLGLGRNQISLPAQIATELGFHRRFSVCLSSTNGVVFPDSGNLDSVYGSEISSSL 215

Query: 466 TYTPILTKKIDGSQSPEYFINVKAIKVDGNRLDLNKSLLDLDGGEGVGGTRLSTVVPYTA 525
           TYTPILTKKID SQSPEYFINVKAIKVDGNRLDLNKSLLDLDGG G GGTRLSTVVPYT 
Sbjct: 216 TYTPILTKKIDASQSPEYFINVKAIKVDGNRLDLNKSLLDLDGGSG-GGTRLSTVVPYTT 275

Query: 526 LESSIFKSLTAAFRAAAAAMNIKEVAPVAPFEACFAAENMEMTATGPKVPEIELILQSEM 585
           LESSIF SLTAAFRAAAAAMN+KEVAPVAPFEACF +ENMEMTATGPKVPEIELILQSEM
Sbjct: 276 LESSIFNSLTAAFRAAAAAMNMKEVAPVAPFEACFESENMEMTATGPKVPEIELILQSEM 335

Query: 586 VGWKIAGRNSMVKVNDETFCLGFVDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSS 645
           VGWKI GRNSMVKVNDET CLGFVDGGLKP+NAVVLGGYQMEDIVLDFDMGTSMLGFSSS
Sbjct: 336 VGWKINGRNSMVKVNDETLCLGFVDGGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSS 395

Query: 646 LLQRKRSCSEFSPENLLKKTG 667
           LLQRK+SCSEFSPENL  + G
Sbjct: 396 LLQRKKSCSEFSPENLPIRAG 413

BLAST of Spg023844 vs. ExPASy TrEMBL
Match: A0A6J1EQJ3 (basic 7S globulin-like OS=Cucurbita moschata OX=3662 GN=LOC111436893 PE=4 SV=1)

HSP 1 Score: 638.3 bits (1645), Expect = 3.7e-179
Identity = 338/380 (88.95%), Postives = 352/380 (92.63%), Query Frame = 0

Query: 286 DPVTDQYLAAVHHGSPIKPTHLAVDLGGPSLWMACGGSSSSRPIPRRSIQCVAAAGGGGG 345
           D VT+QYLA+V+HGSPIKP HL VDLGGPSLWMACG S+SSR I  RSIQCVAA GGG  
Sbjct: 33  DSVTNQYLASVYHGSPIKPIHLVVDLGGPSLWMACGSSTSSRTIQSRSIQCVAATGGGSR 92

Query: 346 SGSGGGGACDVVAGNPFGALVGKAVLVEDTVAVRSFDRSTAAVIVAVHSCAPRFLLQGLA 405
           SGS GGGAC+VVAGNPFGALVGKA+LVEDTVAVR+ +RSTAAVIVAVHSCAPRFLLQGLA
Sbjct: 93  SGS-GGGACEVVAGNPFGALVGKALLVEDTVAVRALERSTAAVIVAVHSCAPRFLLQGLA 152

Query: 406 KSARGILGLGRNQNSLPAQIATELGFHRRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSL 465
           KSA+GILGLGRNQ SLPAQIATELGFHRRFSVCLSSTNGVVFPDS SQDSVYGSEISSSL
Sbjct: 153 KSAKGILGLGRNQISLPAQIATELGFHRRFSVCLSSTNGVVFPDSRSQDSVYGSEISSSL 212

Query: 466 TYTPILTKKIDGSQSPEYFINVKAIKVDGNRLDLNKSLLDLDGGEGVGGTRLSTVVPYTA 525
           TYTPILTK IDGSQS EYFINVKAIKVDGNRLDLNKSLLDLDG +G GGTRLSTVVPYTA
Sbjct: 213 TYTPILTKIIDGSQSAEYFINVKAIKVDGNRLDLNKSLLDLDGEDG-GGTRLSTVVPYTA 272

Query: 526 LESSIFKSLTAAFRAAAAAMNIKEVAPVAPFEACFAAENMEMTATGPKVPEIELILQSEM 585
           LESSIFKSLTAAFR AA AMN+KEVAPVA FEACF++ENMEMTA GPKVP IELILQSEM
Sbjct: 273 LESSIFKSLTAAFREAARAMNMKEVAPVASFEACFSSENMEMTAAGPKVPMIELILQSEM 332

Query: 586 VGWKIAGRNSMVKVNDETFCLGFVDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSS 645
           V WKI GRNSMVKVNDETFCL  VDGGLKPKNAVVLGGYQMEDIVLDFDMG+SMLGFSSS
Sbjct: 333 VRWKIDGRNSMVKVNDETFCLAIVDGGLKPKNAVVLGGYQMEDIVLDFDMGSSMLGFSSS 392

Query: 646 LLQRKRSCSEFSPENLLKKT 666
           LLQRKRSCSEFSPENLLK T
Sbjct: 393 LLQRKRSCSEFSPENLLKST 410

BLAST of Spg023844 vs. ExPASy TrEMBL
Match: A0A0A0KHJ8 (Basic 7S globulin 2 small subunit OS=Cucumis sativus OX=3659 GN=Csa_6G237600 PE=4 SV=1)

HSP 1 Score: 637.1 bits (1642), Expect = 8.2e-179
Identity = 334/384 (86.98%), Postives = 351/384 (91.41%), Query Frame = 0

Query: 286 DPVTDQYLAAVHHGSPIKPTHLAVDLGGPSLWMACGGSSSSRPIPRRSIQCVAAAGGGGG 345
           D +T+QY+A V HGSPIKP HLAVDLGG SLWMACGGSSSSR IP RSIQC+AA  GGG 
Sbjct: 33  DSLTNQYVATVFHGSPIKPVHLAVDLGGQSLWMACGGSSSSRSIPSRSIQCIAAT-GGGR 92

Query: 346 SGSGGGGACDVVAGNPFGALVGKAVLVEDTVAVRSFDRSTAAVIVAVHSCAPRFLLQGLA 405
           SGS  GGACDV+AGNPFG L GKA+LVEDTVAVRS DRSTAAVIVA+HSCAPRFLLQGLA
Sbjct: 93  SGS-VGGACDVIAGNPFGDLEGKAILVEDTVAVRSLDRSTAAVIVALHSCAPRFLLQGLA 152

Query: 406 KSARGILGLGRNQNSLPAQIATELGFHRRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSL 465
           KS +G+LGLGRNQ SLPAQIATELG HRRFS+CLSSTNGVVFPDSGSQDSVYGSEISSSL
Sbjct: 153 KSVKGVLGLGRNQISLPAQIATELGSHRRFSLCLSSTNGVVFPDSGSQDSVYGSEISSSL 212

Query: 466 TYTPILTKKIDGSQSPEYFINVKAIKVDGNRLDLNKSLLDLDG---GEGVGGTRLSTVVP 525
           TYTPILTKKID  QSPEYFINVKAIKVDGNRLDLNKSLLDL+G   GEG GGTRLSTVVP
Sbjct: 213 TYTPILTKKIDALQSPEYFINVKAIKVDGNRLDLNKSLLDLEGVGDGEGGGGTRLSTVVP 272

Query: 526 YTALESSIFKSLTAAFRAAAAAMNIKEVAPVAPFEACFAAENMEMTATGPKVPEIELILQ 585
           YT LESSIF SLTAAFRAAAAAMN+KEVAPVAPFE CF +ENMEMTA GPKVPEIELILQ
Sbjct: 273 YTVLESSIFNSLTAAFRAAAAAMNMKEVAPVAPFEVCFESENMEMTAAGPKVPEIELILQ 332

Query: 586 SEMVGWKIAGRNSMVKVNDETFCLGFVDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGF 645
           SEMVGWKI GRNSMVKVNDE +CLGFVDGGLKP+NA+VLGGYQMEDIVLDFDMGTSMLGF
Sbjct: 333 SEMVGWKIYGRNSMVKVNDEAYCLGFVDGGLKPRNAIVLGGYQMEDIVLDFDMGTSMLGF 392

Query: 646 SSSLLQRKRSCSEFSPENLLKKTG 667
           SSSLLQRKRSCSEFSP NLL+ TG
Sbjct: 393 SSSLLQRKRSCSEFSPVNLLESTG 414

BLAST of Spg023844 vs. ExPASy TrEMBL
Match: A0A6J1L1S1 (basic 7S globulin-like OS=Cucurbita maxima OX=3661 GN=LOC111499071 PE=4 SV=1)

HSP 1 Score: 632.1 bits (1629), Expect = 2.6e-177
Identity = 332/381 (87.14%), Postives = 347/381 (91.08%), Query Frame = 0

Query: 286 DPVTDQYLAAVHHGSPIKPTHLAVDLGGPSLWMACGGSSSSRPIPRRSIQCVAAAGGGGG 345
           D VT+QYLA ++HGS IKP HLAVDLGG  LWMACGGSSSSRPI  RSIQC+AA GGG  
Sbjct: 34  DSVTNQYLATLYHGSQIKPVHLAVDLGGRFLWMACGGSSSSRPILSRSIQCIAAVGGGRS 93

Query: 346 SGSGGGGACDVVAGNPFGALVGKAVLVEDTVAVRSFDRSTAAVIVAVHSCAPRFLLQGLA 405
             S  G ACDV+AGNPFG L GKAVLVEDTVAVRS DRSTAAVIVA+HSCAPRFLLQGLA
Sbjct: 94  GSS--GSACDVIAGNPFGDLEGKAVLVEDTVAVRSLDRSTAAVIVALHSCAPRFLLQGLA 153

Query: 406 KSARGILGLGRNQNSLPAQIATELGFHRRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSL 465
           KSA+G+LGLGRNQ SLPAQIATELGFHRRFSVCLSSTNGVVFPD+G+ DSVYGSEISSSL
Sbjct: 154 KSAKGVLGLGRNQISLPAQIATELGFHRRFSVCLSSTNGVVFPDTGNLDSVYGSEISSSL 213

Query: 466 TYTPILTKKIDGSQSPEYFINVKAIKVDGNRLDLNKSLLDLDGGEGVGGTRLSTVVPYTA 525
           TYTPILTKK D SQSPEYFINVKAIKVDGNRLDLNKSLLDLDGG G GGTRLSTVVPYT 
Sbjct: 214 TYTPILTKKTDASQSPEYFINVKAIKVDGNRLDLNKSLLDLDGGGG-GGTRLSTVVPYTT 273

Query: 526 LESSIFKSLTAAFRAAAAAMNIKEVAPVAPFEACFAAENMEMTATGPKVPEIELILQSEM 585
           L SSIF SLTAAFRAAAAAMN+KEVAPVAPFEACF +ENMEMTATGPKVPEIELILQSEM
Sbjct: 274 LGSSIFNSLTAAFRAAAAAMNMKEVAPVAPFEACFESENMEMTATGPKVPEIELILQSEM 333

Query: 586 VGWKIAGRNSMVKVNDETFCLGFVDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSS 645
           VGWKI GRNSMVKVNDET CLGFVDGGLKP+NAVVLGGYQMEDIVLDFDMGTSMLGFSSS
Sbjct: 334 VGWKINGRNSMVKVNDETLCLGFVDGGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSS 393

Query: 646 LLQRKRSCSEFSPENLLKKTG 667
           LLQRK+SCSEFSPENL  + G
Sbjct: 394 LLQRKKSCSEFSPENLPIRAG 411

BLAST of Spg023844 vs. ExPASy TrEMBL
Match: A0A6J1KM09 (basic 7S globulin-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111494504 PE=4 SV=1)

HSP 1 Score: 626.3 bits (1614), Expect = 1.4e-175
Identity = 333/380 (87.63%), Postives = 349/380 (91.84%), Query Frame = 0

Query: 286 DPVTDQYLAAVHHGSPIKPTHLAVDLGGPSLWMACGGSSSSRPIPRRSIQCVAAAGGGGG 345
           D VT+QYLA+V+HGSPIKP HLAVDLGGPSLWMACG S+SSR I  RSIQCVAA G G  
Sbjct: 33  DSVTNQYLASVYHGSPIKPIHLAVDLGGPSLWMACGSSTSSRTIQSRSIQCVAATGDGSR 92

Query: 346 SGSGGGGACDVVAGNPFGALVGKAVLVEDTVAVRSFDRSTAAVIVAVHSCAPRFLLQGLA 405
           SGS GGGAC+VVA NPFGALVGKA+LVEDTVAVR+  RSTAAVIVAVHSCAPRFLLQGLA
Sbjct: 93  SGS-GGGACEVVARNPFGALVGKALLVEDTVAVRALARSTAAVIVAVHSCAPRFLLQGLA 152

Query: 406 KSARGILGLGRNQNSLPAQIATELGFHRRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSL 465
           KSA+GILGLGRNQ SLPAQIATELGFHRRFSVCLSSTNGVVFP+SGSQDSVYGSEIS+SL
Sbjct: 153 KSAKGILGLGRNQISLPAQIATELGFHRRFSVCLSSTNGVVFPESGSQDSVYGSEISNSL 212

Query: 466 TYTPILTKKIDGSQSPEYFINVKAIKVDGNRLDLNKSLLDLDGGEGVGGTRLSTVVPYTA 525
           TYTPILTK IDGSQS EYFINVKAIKVDGNRL L+KSLLDLD  +G GGTRLSTVVPYTA
Sbjct: 213 TYTPILTKIIDGSQSAEYFINVKAIKVDGNRLVLDKSLLDLDSEDG-GGTRLSTVVPYTA 272

Query: 526 LESSIFKSLTAAFRAAAAAMNIKEVAPVAPFEACFAAENMEMTATGPKVPEIELILQSEM 585
           LESSIFKSLTAAFR AA AMN+KEVAPVA FE CFA+ENMEMTATGPKVP+IELILQSEM
Sbjct: 273 LESSIFKSLTAAFREAARAMNMKEVAPVASFEVCFASENMEMTATGPKVPKIELILQSEM 332

Query: 586 VGWKIAGRNSMVKVNDETFCLGFVDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSS 645
           V WK  GRNSMVKVNDETFCL  VDGGLKPKNAVVLGGYQMEDIVLDFDMG+SMLGFSSS
Sbjct: 333 VRWKFDGRNSMVKVNDETFCLAIVDGGLKPKNAVVLGGYQMEDIVLDFDMGSSMLGFSSS 392

Query: 646 LLQRKRSCSEFSPENLLKKT 666
           LLQRKRSCSEFSPENLLK T
Sbjct: 393 LLQRKRSCSEFSPENLLKST 410

BLAST of Spg023844 vs. TAIR 10
Match: AT1G03220.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 257.3 bits (656), Expect = 3.4e-68
Identity = 162/409 (39.61%), Postives = 236/409 (57.70%), Query Frame = 0

Query: 278 PSLPSGVVDPVTD-----QYLAAVHHGSPIKPTHLAVDLGGPSLWMAC--GGSSSSRPIP 337
           P  P  ++ PVT      QY   ++  +P+ P  +  DLGG  LW+ C  G  SS+   P
Sbjct: 25  PFRPKALLLPVTKDQSTLQYTTVINQRTPLVPASVVFDLGGRELWVDCDKGYVSSTYQSP 84

Query: 338 RRSIQCVAAAGGGGGSGSGG--------GGACDVVAGNPFGALVGKAVLVE---DTVAVR 397
           R    C +A     GS S G        G + +   G P   + G A   E   D V+++
Sbjct: 85  R----CNSAVCSRAGSTSCGTCFSPPRPGCSNNTCGGIPDNTVTGTATSGEFALDVVSIQ 144

Query: 398 SFDRSTAAVIV----AVHSCAPRFLLQGLAKSARGILGLGRNQNSLPAQIATELGFHRRF 457
           S + S    +V     +  C   FLL+GLAK   G+ G+GR+   LP+Q A    FHR+F
Sbjct: 145 STNGSNPGRVVKIPNLIFDCGATFLLKGLAKGTVGMAGMGRHNIGLPSQFAAAFSFHRKF 204

Query: 458 SVCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKI-------DGSQSPEYFINVK 517
           +VCL+S  GV F  +G    + G +I SSL  TP+L   +        G +S EYFI V 
Sbjct: 205 AVCLTSGKGVAFFGNGPYVFLPGIQI-SSLQTTPLLINPVSTASAFSQGEKSSEYFIGVT 264

Query: 518 AIKVDGNRLDLNKSLLDLDGGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNIK 577
           AI++    + +N +LL ++   G+GGT++S+V PYT LESSI+ + T+ F   AAA +IK
Sbjct: 265 AIQIVEKTVPINPTLLKINASTGIGGTKISSVNPYTVLESSIYNAFTSEFVKQAAARSIK 324

Query: 578 EVAPVAPFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDETFCLGF 637
            VA V PF ACF+ +N+ +T  G  VPEIEL+L S+ V W+I G NSMV V+D+  CLGF
Sbjct: 325 RVASVKPFGACFSTKNVGVTRLGYAVPEIELVLHSKDVVWRIFGANSMVSVSDDVICLGF 384

Query: 638 VDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFS 658
           VDGG+  + +VV+GG+Q+ED +++FD+ ++  GFSS+LL R+ +C+ F+
Sbjct: 385 VDGGVNARTSVVIGGFQLEDNLIEFDLASNKFGFSSTLLGRQTNCANFN 428

BLAST of Spg023844 vs. TAIR 10
Match: AT1G03230.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 250.0 bits (637), Expect = 5.4e-66
Identity = 161/405 (39.75%), Postives = 232/405 (57.28%), Query Frame = 0

Query: 275 PSAPSLPSGVVDPVTDQYLAAVHHGSPIKPTHLAVDLGGPSLWMACGGSSSSRPIPRRSI 334
           P A  LP    DP T QY   ++  +P+ P  +  DLGG   W+ C     S     RS 
Sbjct: 29  PKALLLPV-TKDPSTLQYTTVINQRTPLVPASVVFDLGGREFWVDCDQGYVSTTY--RSP 88

Query: 335 QCVAAAGGGGGSGSGG--------GGACDVVAGNPFGALVGKAVLVE---DTVAVRSFDR 394
           +C +A     GS + G        G + +     P  ++ G A   E   D V+++S + 
Sbjct: 89  RCNSAVCSRAGSIACGTCFSPPRPGCSNNTCGAFPDNSITGWATSGEFALDVVSIQSTNG 148

Query: 395 STAAVIV----AVHSCAPRFLLQGLAKSARGILGLGRNQNSLPAQIATELGFHRRFSVCL 454
           S     V     + SC    LL+GLAK A G+ G+GR+   LP Q A    F+R+F+VCL
Sbjct: 149 SNPGRFVKIPNLIFSCGSTSLLKGLAKGAVGMAGMGRHNIGLPLQFAAAFSFNRKFAVCL 208

Query: 455 SSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKK-------IDGSQSPEYFINVKAIKV 514
           +S  GV F  +G    + G +I S L  TP+L            G +SPEYFI V AIK+
Sbjct: 209 TSGRGVAFFGNGPYVFLPGIQI-SRLQKTPLLINPGTTVFEFSKGEKSPEYFIGVTAIKI 268

Query: 515 DGNRLDLNKSLLDLDGGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNIKEVAP 574
               L ++ +LL ++   G+GGT++S+V PYT LESSI+K+ T+ F   AAA +IK VA 
Sbjct: 269 VEKTLPIDPTLLKINASTGIGGTKISSVNPYTVLESSIYKAFTSEFIRQAAARSIKRVAS 328

Query: 575 VAPFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDETFCLGFVDGG 634
           V PF ACF+ +N+ +T  G  VPEI+L+L S+ V W+I G NSMV V+D+  CLGFVDGG
Sbjct: 329 VKPFGACFSTKNVGVTRLGYAVPEIQLVLHSKDVVWRIFGANSMVSVSDDVICLGFVDGG 388

Query: 635 LKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFS 658
           + P  +VV+GG+Q+ED +++FD+ ++  GFSS+LL R+ +C+ F+
Sbjct: 389 VNPGASVVIGGFQLEDNLIEFDLASNKFGFSSTLLGRQTNCANFN 429

BLAST of Spg023844 vs. TAIR 10
Match: AT5G19120.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 211.1 bits (536), Expect = 2.8e-54
Identity = 154/387 (39.79%), Postives = 200/387 (51.68%), Query Frame = 0

Query: 282 SGVVDPV-----TDQYLAAVHHGSPIKPTHLAVDLGGPSLWMACGG---SSSSRPIPRRS 341
           +GVV PV     T QYLA +  G    P  L VDL G  LW  C     SSS   I   S
Sbjct: 30  NGVVFPVVKDLPTGQYLAQIRLGDSPDPVKLVVDLAGSILWFDCSSRHVSSSRNLISGSS 89

Query: 342 IQCVAAAGG------GGGSGSGGGGACDVVAGNPFGALVGKAVLVEDTVAVRSFDRSTAA 401
             C+ A  G         S       C+++  N    +  +  L  D ++V S   ++  
Sbjct: 90  SGCLKAKVGNERVSSSSSSRKDQNADCELLVKNDAFGITARGELFSDVMSVGSV--TSPG 149

Query: 402 VIVAVHSCAPRFLLQGLAKSARGILGLGRNQNSLPAQIATELGFHRRFSVCLSSTNGVVF 461
            +  + +C P +LL+GLA  A+G++GLGR Q SLP+Q+A E    RR +V LS  NGVV 
Sbjct: 150 TVDLLFACTPPWLLRGLASGAQGVMGLGRAQISLPSQLAAETNERRRLTVYLSPLNGVV- 209

Query: 462 PDSGSQDSVYGSEISSSLTYTPILTKKIDGSQSPEYFINVKAIKVDGNRLDLNKSLLDLD 521
             + S + V+G   S SL YTP+LT       S  Y INVK+I+V+G +L +        
Sbjct: 210 -STSSVEEVFGVAASRSLVYTPLLT-----GSSGNYVINVKSIRVNGEKLSV-------- 269

Query: 522 GGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNIKEVAPVAPFEACFAAENMEM 581
             EG     LSTVVPYT LESSI+K    A+  A AA     V PVAPF  CF ++    
Sbjct: 270 --EGPLAVELSTVVPYTILESSIYKVFAEAY--AKAAGEATSVPPVAPFGLCFTSD---- 329

Query: 582 TATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDETFCLGFVDGGLKPKNAVVLGGYQME 641
                  P ++L LQSEMV W+I G+N MV V     C G VDGG    N +V+GG Q+E
Sbjct: 330 ----VDFPAVDLALQSEMVRWRIHGKNLMVDVGGGVRCSGIVDGGSSRVNPIVMGGLQLE 383

Query: 642 DIVLDFDMGTSMLGFSSSLLQRKRSCS 655
             +LDFD+G SM+GF     QR RS S
Sbjct: 390 GFILDFDLGNSMMGFG----QRTRSDS 383

BLAST of Spg023844 vs. TAIR 10
Match: AT5G19110.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 162.5 bits (410), Expect = 1.1e-39
Identity = 129/380 (33.95%), Postives = 185/380 (48.68%), Query Frame = 0

Query: 289 TDQYLAAVHHGSPIK-PTHLAVDLGGPSLWMAC---GGSSSSRPIPRRSIQCVAAAGGGG 348
           T+ +    + GS  K P +L +DLG    W+ C      SS R +  +S  C +  G G 
Sbjct: 36  TNLFYTTFNVGSAAKSPVNLLLDLGTNLTWLDCRKLKSLSSLRLVTCQSSTCKSIPGNG- 95

Query: 349 GSGSGGGGACDVVAGNPFG---ALVGKAVLVEDTVAVRSFDRSTAAVIVAVH----SCAP 408
                 G +C     NP G    + G+  +V+D  ++ + D       V+V     SCA 
Sbjct: 96  ----CAGKSCLYKQPNPLGQNPVVTGR--VVQDRASLYTTDGGKFLSQVSVRHFTFSCAG 155

Query: 409 RFLLQGLAKSARGILGLGRNQNSLPAQIATELGFHRRFSVCLSSTNGVVFPDSGSQDSVY 468
              LQGL     G+L L    +S   Q+ +      +FS+CL S+    F  +G     +
Sbjct: 156 EKALQGLPPPVDGVLALSPGSSSFTKQVTSAFNVIPKFSLCLPSSGTGHFYIAGIH--YF 215

Query: 469 GSEISSSLTYTPILTKKIDGSQSPEYFINVKAIKVDGNRLDLNKSLLDLDGGEGVGGTRL 528
               +SS    P     I G+ S +Y I VK+I V G  L LN  LL        GG +L
Sbjct: 216 IPPFNSSDNPIPRTLTPIKGTDSGDYLITVKSIYVGGTALKLNPDLL-------TGGAKL 275

Query: 529 STVVPYTALESSIFKSLTAAFRAAAAAMNIKEVAPVAPFEACFAAENMEMTAT-GPKVPE 588
           STVV YT L++ I+ +L  +F   A AM I +V  VAPF+ CF +       T GP VP 
Sbjct: 276 STVVHYTVLQTDIYNALAQSFTLKAKAMGIAKVPSVAPFKHCFDSRTAGKNLTAGPNVPV 335

Query: 589 IELILQSEM--VGWKIAGRNSMVKVNDETFCLGFVDGGLKPKNAVVLGGYQMEDIVLDFD 648
           IE+ L   +  V W   G N++VKV +   CL F+DGG  PK+ +V+G +Q++D +L+FD
Sbjct: 336 IEIGLPGRIGEVKWGFYGANTVVKVKETVMCLAFIDGGKTPKDLMVIGTHQLQDHMLEFD 395

Query: 649 MGTSMLGFSSSLLQRKRSCS 655
              ++L FS SLL    SCS
Sbjct: 396 FSGTVLAFSESLLLHNTSCS 399

BLAST of Spg023844 vs. TAIR 10
Match: AT5G19100.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 138.7 bits (348), Expect = 1.8e-32
Identity = 89/250 (35.60%), Postives = 133/250 (53.20%), Query Frame = 0

Query: 412 LGLGRNQNSLPAQIATELGFHRRFSVCLSST------NGVVFPDSGSQDSV-YGSEISSS 471
           +GL     S+P+Q+ +      + ++CL ST      NG ++   G    + Y  ++S  
Sbjct: 160 IGLANTHLSIPSQLISMYQLPHKIALCLPSTERSQSHNGDLWIGKGEYYYLPYDKDVSKI 219

Query: 472 LTYTPILTKKIDGSQSPEYFINVKAIKVDGNRLDLNKSLLDLDGGEGVGGTRLSTVVPYT 531
              TP+    I   +S EY I+VK+I++    + +             G T++ST+ PYT
Sbjct: 220 FASTPL----IGNGKSGEYLIDVKSIQIGAKTVPI-----------PYGATKISTLAPYT 279

Query: 532 ALESSIFKSLTAAFRAAAAAMNIKEVAPVAPFEACFAAENMEMTATGPKVPEIELILQSE 591
             ++S++K+L  AF      + I +   V PF ACF +        G  VP I+L+L S 
Sbjct: 280 VFQTSLYKALLTAF---TENIKIAKAPAVKPFGACFYSNG------GRGVPVIDLVL-SG 339

Query: 592 MVGWKIAGRNSMVKVNDETFCLGFVDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSS 651
              W+I G NS+VKVN    CLGFVDGG+KPK  +V+GG+QMED +++FD+  S   FSS
Sbjct: 340 GAKWRIYGSNSLVKVNKNVVCLGFVDGGVKPKYPIVIGGFQMEDNLVEFDLEASKFSFSS 384

Query: 652 SLLQRKRSCS 655
           SLL    SCS
Sbjct: 400 SLLLHNTSCS 384

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7026255.18.9e-18089.21Basic 7S globulin, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022959401.11.5e-17987.93basic 7S globulin-like [Cucurbita moschata][more]
KAG7013583.14.4e-17987.50Basic 7S globulin, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022930452.17.6e-17988.95basic 7S globulin-like [Cucurbita moschata][more]
KAG6593913.17.6e-17988.68putative aspartic proteinase GIP2, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
P0DO216.2e-6739.46Probable aspartic proteinase GIP2 OS=Nicotiana benthamiana OX=4100 GN=GIP2 PE=1 ... [more]
I1JNS63.2e-5536.20Probable aspartic proteinase GIP1 OS=Glycine max OX=3847 GN=GIP1 PE=1 SV=2[more]
P829525.5e-4734.27Gamma conglutin 1 OS=Prunus dulcis OX=3755 GN=Cgamma1 PE=1 SV=2[more]
Q9FSH97.4e-3630.38Gamma conglutin 1 OS=Lupinus albus OX=3870 GN=Cgamma PE=1 SV=1[more]
Q423694.1e-3430.75Gamma conglutin 1 OS=Lupinus angustifolius OX=3871 GN=LOC109345795 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1H4R87.4e-18087.93basic 7S globulin-like OS=Cucurbita moschata OX=3662 GN=LOC111460386 PE=4 SV=1[more]
A0A6J1EQJ33.7e-17988.95basic 7S globulin-like OS=Cucurbita moschata OX=3662 GN=LOC111436893 PE=4 SV=1[more]
A0A0A0KHJ88.2e-17986.98Basic 7S globulin 2 small subunit OS=Cucumis sativus OX=3659 GN=Csa_6G237600 PE=... [more]
A0A6J1L1S12.6e-17787.14basic 7S globulin-like OS=Cucurbita maxima OX=3661 GN=LOC111499071 PE=4 SV=1[more]
A0A6J1KM091.4e-17587.63basic 7S globulin-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111494504 PE... [more]
Match NameE-valueIdentityDescription
AT1G03220.13.4e-6839.61Eukaryotic aspartyl protease family protein [more]
AT1G03230.15.4e-6639.75Eukaryotic aspartyl protease family protein [more]
AT5G19120.12.8e-5439.79Eukaryotic aspartyl protease family protein [more]
AT5G19110.11.1e-3933.95Eukaryotic aspartyl protease family protein [more]
AT5G19100.11.8e-3235.60Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 284..448
e-value: 6.3E-27
score: 96.7
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 456..659
e-value: 1.3E-59
score: 203.1
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 286..647
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 292..443
e-value: 2.3E-23
score: 83.3
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 482..643
e-value: 3.3E-52
score: 176.5
IPR001461Aspartic peptidase A1 familyPANTHERPTHR47965ASPARTYL PROTEASE-RELATEDcoord: 286..656
NoneNo IPR availablePANTHERPTHR47965:SF46BASIC 7S GLOBULIN 2 SMALL SUBUNITcoord: 286..656
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 292..643
score: 17.44318

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg023844.1Spg023844.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity