Spg023687 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg023687
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionFlap endonuclease GEN-like 1
Locationscaffold13: 8377568 .. 8381735 (+)
RNA-Seq ExpressionSpg023687
SyntenySpg023687
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAAAAAAAAAAAGTTACCGTTTCAAATTGACGATGATGAAGCTACGGGCCCAAAATCGGGGCAGCCCAAATCCCATTTATGGAATTTTAAGGGAAAAAAACATGGCCCTTTCTAAACGTTGTAACGGTAACAAACCATCAACATTCTGAAAAATTGAAGTTTCTGCTAACAGACGAATGAAGATGAACCTCTTCTTCTCAAAGCATCAATCAATCACTACAACGAAACACTGCAACTTCCTCTTGATTTCATGAACCCATTTCTGAATCACTCGAAGCAATGGGAGTTGGAGGTCATTTCTGGGATTTACTCAAACCTCATGCTCGAACTGAGGGCTTCGATTTCCTCAGGAACAAACGGATCGCCGTCGACCTCTCTTTCTGGATCGTACAGCACGAGACCGCCATTAAAGCCAGTGCTCGAAGCCCCCATTTGAGGCTCACATTCTTCCGCACCATCACTCTCTTTGCTAAGGTGTTGTTTTAGTGTTTTCTTTCTTTGGGTGCAAGAATTCGGCGGTGAAATTCTTTTACCGTTGTGGGAATTGATGTTTTCGGTGTAAGAATTCGAGGGAGTTTTTGGGTTTTGTGAAGTGTTTGTGTCTATGCCCTTTTGTTTGTTGCATGATGAGTAACGTGAAGGAATTTGAAAAGAAATTGGAATTCCGTTGGTTGTAATTCTTCTGGTGTAAGATGATTATGATTCTCTCTTTTTGTTTTTCTTCGATGGCATCAGTAAGTTGTTGGTTTATGGTGGGATTTGGTTCAAGCTGATGGTGATTCTGTTGTGTATTTTGCAGTTTGGTGCCTTTCCTGTGTTTGTAGTTGATGGAACTCCTTCACCCTTGAAATCTAAGGCAAGAATTGCGAGATTCTTCCGGTTGTCTGGTATTGACACTTCTGATCTGCCGAAGATTGAAGATCGCATTTCAGTTGACAGGAATCGCAAGTTTGCAAAATGTGTTAAAGAATGTGTGGTAGGGTGTTTCACTTGCTTTGACTATTTTCATCATATATATGTCCATCAATTTAGAATCCATTCGATCTTATTTGCTTTTGAACGAGAATCATATGGTTTTGTGCATCCGTTCCAAGGGCTTGGGATGTTTGGAGATACCCTCCAAAGTCCTAATTCGAGCCTCAAGGTGAAAACTAAAAAAAATTTAAGTTATTGTTGTCTCTGGGAGTTGACCTTGGGTGTGCGAAGTCTCCCAAAGAATAACGAGGTCGGCAATGAGCATAATGCAAGGAGTGGTATCACCTGATACTTTGGTTATAGAAAAGAAATGGTTTTGTGCATCTTGGGTTTTTGTTATTGACTTCCATCATGTAAAGTCTCTTCAATGTTGGTGTAGGTGAGCTTTGTCGTCTTTGCATGTGCATAAAGTTTTGGGTTCCTATGTGCCATCTTTTCTTATTGTGATACTCACTGCATATTTGTGATTCTTTCGTTTGATTTGATGGACTTGCAAAGCTCATCATGTGTTCTTACTAATAGTTTCTTCATAGGAACTTCTTGAGCTATTTGGGGTGCCCGTCATAGAAGCAAAAGGAGAAGCCGAAGCACTGTGCGCCGAGTTGAATAGGAAAGGCTTTGTAGATGCCTGCATCACCGCTGACAGTGATGCTTTTCTGTTTGGTGCCAAATGTGTGATTAAAAGCTTCCATCCCAATTCAAAAGCAAGTACTTTTATCTATACTAGTCTTCTTTTTATATGTGACTTCTTTCTTACCATTTTCTCTTGAATATCATCACATGCAGGAACCACTTGAATGCTACTTCATGTCAGATATTGAGGCTGCTCTTGGGTTGAATAGGAAGCATTTGATAGCAATCTCTCTCCTGGTTGGAAATGACCATGACTTAAAAGGAGTGCAAGGAGTTGGACTAGATACTGCCGTTCGTTTCGTCCAAGATTATACAGATGATGAGATTTTAAATAAGTAATATTTAGCTTACATGTTCTTTTCAAATACCAAAAGGTAACTTTATGATTATTATTCGATATTTTAACTGTGTTCGAACTGCTGAGTGTTATGCAGATTGCATGAAATAGGCAATGAGGATAATACGCTGCTTCAAGGTGGTCTTGAATTGGTAAATAACTATGGACAATCTTCGGACACGAACTCAAGAAAGATCAAGTGTTCTCACTGTTCCTTCTGTGGTCATCCAGGCAGCAAAAGAGCTCATGTTAAGTTCCCTTGTGAATACTGTGATGTGAGTCATGGCGATGGTTGCATCAAAAAGCCGGATGGATTTAAATGCAATTGTTCCTCATGTGATACGGTACCTTCTGATCGAGTTTCTTTCTGTTTGATTTTTTCTCTACATTGTGGTTTGTTGGATTCTCTACAATTACGTTGTGGTCTTTGTGCAGCATCCGTTATTTTGTAAAGGTTTCTGCTTTAGTTGCTTCTTTTACTTAAGCAATATGTATTTTGGTTTTTGTCCAATATTGTTTAAAAGACATTTTTTGGTTCATGATTGGCAGGATCGAGAAGAAAAAGAGAAAAAGAAGCAAGAAAATTGGCGATTAAGAGTTTGCAACAAGATTGCCATGGAGCCAAATTTTCCCAGTAGTGAACTTGTTCAAATATACCTGGGCGACAATCATAGTTATTTTAGTGGTATGAGTCCACATTTGCATTTTAAGTAGCAAAAATTGTTACCGATGTTTTCTATTTGATGAACAAATCTCTGGTGCTGCTATGACCCCGTGACTGATTAATTGAAACGCATCCATTCGGGCAGCAAATGGGCGTTATTTTCAAATTTTGCTGTGTTTTGGAACAAACACCTCCTATTTTAAATGAAACGATGCTTACATGCTTGTTTAAGGAGGCCTTTTCAATCTTTAAATTAGTTAGTGTCTAAGATTGTTTTGAATAACTTTTTTGCACATGTTAAGCTTTTATCTCATTTGGTCCCTTTCCACTGCAGATGATGACCTTATATTATCTTGGGGAAGCCCGAGAACCGAAATGCTGATTGATTTTTTAGTTTTTCATCTACGGTGGGAGCCATCTTACATTCGTCAAAGGATGCTTCCCATGCTATCCACCATTTTTCTCAGAGAAAAGGCTAATAATCCAATTCAAACTTTGTTATACGGCCAGTACGAGTTTGATTCCATACTTAGAGAAAAAATAAGATATGGACATCCGTTTTATGTTGTCAAATGGAAAAAGGCCACCCCTACACTCGGCAATGTTGTAAACGAAGGCTCTTCCGGGGGATTTGGCACTGGACCAGAAGATGCCATTGACGTCGATGAACCAGTGGATTTAACGGATGAATCTGATTCCCCAGAAATTCATATTCAAGACGGGTGCTCCTTTTTGTTGACCGACGAGAATATGGACCTTGTACGTGCTGCATATCCTGAACAGGCTGCCAAATTTTTGCAGGAAATGGTAATCATCCTTTGTTTTTAGTTCTATGCCTCATTTCTTCCCTGTCTTTGATCTTTGTACTGTAGCTTTGCTTTTGTTCTAACTATACTATTGAAGTGGGAATGGCTGAGCTCTAAAGACAGTTCATTTTTTCAATGTTTACAAACTAGCTTTAGTTGATGCTTGGGATTGTATGTGAATCTACTGATGTTGATGTCTTAAGCTGCAATGAATAAATTTCATTCATGTTTGAAAATTTCTTGGAACTAAAGGAAGTGAAAAGGCAAAAGACACCAGCCACTCCACCTTGCGGCGGGACTAGCGGAAAGTCTGATTCATCGAAGGCGAAAGGTGTCCAGTTAAGTATAACCGAGTTCTATCGTGCAACCAAAACACAACAAGCAACGGATGCAGGAGATTCGACAAATGAGGGCGAGGAGACAAGTACTGGAAAGAGGAAATCATCAAGTTCTACTCTTTCAAAGTCGGTAAGACGTCGCCTTTTGTTCGAGGACTAGAGCTCTTTGAGCTATTGTGTGTGCCCTGTCTGATTGAGTGCAAATCCTGCTGTTTGAATATGTATAAAGTTTCTAGCTAATCTAGATCTTTTGAGCTTTCCTTTGTTAAGCATTGATTAGTGCTTATAATGTTGTAATTAGAATAGATTAGATTTATCAAAGATTTCAATTTAGTCCCTATAATTTGGTTAAATCTTCCAATCTGTTACTACCATTGAGAGGAGGTATTTCTTTATTATATTCAAATTTAG

mRNA sequence

ATGGGAGTTGGAGGTCATTTCTGGGATTTACTCAAACCTCATGCTCGAACTGAGGGCTTCGATTTCCTCAGGAACAAACGGATCGCCGTCGACCTCTCTTTCTGGATCGTACAGCACGAGACCGCCATTAAAGCCAGTGCTCGAAGCCCCCATTTGAGGCTCACATTCTTCCGCACCATCACTCTCTTTGCTAAGTTTGGTGCCTTTCCTGTGTTTGTAGTTGATGGAACTCCTTCACCCTTGAAATCTAAGGCAAGAATTGCGAGATTCTTCCGGTTGTCTGGTATTGACACTTCTGATCTGCCGAAGATTGAAGATCGCATTTCAGTTGACAGGAATCGCAAGTTTGCAAAATGTGTTAAAGAATGTGTGGAACTTCTTGAGCTATTTGGGGTGCCCGTCATAGAAGCAAAAGGAGAAGCCGAAGCACTGTGCGCCGAGTTGAATAGGAAAGGCTTTGTAGATGCCTGCATCACCGCTGACAGTGATGCTTTTCTGTTTGGTGCCAAATGTGTGATTAAAAGCTTCCATCCCAATTCAAAAGCAAATATTGAGGCTGCTCTTGGGTTGAATAGGAAGCATTTGATAGCAATCTCTCTCCTGGTTGGAAATGACCATGACTTAAAAGGAGTGCAAGGAGTTGGACTAGATACTGCCGTTCGTTTCGTCCAAGATTATACAGATGATGAGATTTTAAATAAATTGCATGAAATAGGCAATGAGGATAATACGCTGCTTCAAGGTGGTCTTGAATTGGTAAATAACTATGGACAATCTTCGGACACGAACTCAAGAAAGATCAAGTGTTCTCACTGTTCCTTCTGTGGTCATCCAGGCAGCAAAAGAGCTCATGTTAAGTTCCCTTGTGAATACTGTGATGTGAGTCATGGCGATGGTTGCATCAAAAAGCCGGATGGATTTAAATGCAATTGTTCCTCATGTGATACGGATCGAGAAGAAAAAGAGAAAAAGAAGCAAGAAAATTGGCGATTAAGAGTTTGCAACAAGATTGCCATGGAGCCAAATTTTCCCAGTAGTGAACTTGTTCAAATATACCTGGGCGACAATCATAGTTATTTTAGTGATGATGACCTTATATTATCTTGGGGAAGCCCGAGAACCGAAATGCTGATTGATTTTTTAGTTTTTCATCTACGGTGGGAGCCATCTTACATTCGTCAAAGGATGCTTCCCATGCTATCCACCATTTTTCTCAGAGAAAAGGCTAATAATCCAATTCAAACTTTGTTATACGGCCAGTACGAGTTTGATTCCATACTTAGAGAAAAAATAAGATATGGACATCCGTTTTATGTTGTCAAATGGAAAAAGGCCACCCCTACACTCGGCAATGTTGTAAACGAAGGCTCTTCCGGGGGATTTGGCACTGGACCAGAAGATGCCATTGACGTCGATGAACCAGTGGATTTAACGGATGAATCTGATTCCCCAGAAATTCATATTCAAGACGGGTGCTCCTTTTTGTTGACCGACGAGAATATGGACCTTGTACGTGCTGCATATCCTGAACAGGCTGCCAAATTTTTGCAGGAAATGGAAGTGAAAAGGCAAAAGACACCAGCCACTCCACCTTGCGGCGGGACTAGCGGAAAGTCTGATTCATCGAAGGCGAAAGGTGTCCAGTTAAGTATAACCGAGTTCTATCGTGCAACCAAAACACAACAAGCAACGGATGCAGGAGATTCGACAAATGAGGGCGAGGAGACAAGTACTGGAAAGAGGAAATCATCAAGTTCTACTCTTTCAAAGTCGGTAAGACGTCGCCTTTTGTTCGAGGACTAG

Coding sequence (CDS)

ATGGGAGTTGGAGGTCATTTCTGGGATTTACTCAAACCTCATGCTCGAACTGAGGGCTTCGATTTCCTCAGGAACAAACGGATCGCCGTCGACCTCTCTTTCTGGATCGTACAGCACGAGACCGCCATTAAAGCCAGTGCTCGAAGCCCCCATTTGAGGCTCACATTCTTCCGCACCATCACTCTCTTTGCTAAGTTTGGTGCCTTTCCTGTGTTTGTAGTTGATGGAACTCCTTCACCCTTGAAATCTAAGGCAAGAATTGCGAGATTCTTCCGGTTGTCTGGTATTGACACTTCTGATCTGCCGAAGATTGAAGATCGCATTTCAGTTGACAGGAATCGCAAGTTTGCAAAATGTGTTAAAGAATGTGTGGAACTTCTTGAGCTATTTGGGGTGCCCGTCATAGAAGCAAAAGGAGAAGCCGAAGCACTGTGCGCCGAGTTGAATAGGAAAGGCTTTGTAGATGCCTGCATCACCGCTGACAGTGATGCTTTTCTGTTTGGTGCCAAATGTGTGATTAAAAGCTTCCATCCCAATTCAAAAGCAAATATTGAGGCTGCTCTTGGGTTGAATAGGAAGCATTTGATAGCAATCTCTCTCCTGGTTGGAAATGACCATGACTTAAAAGGAGTGCAAGGAGTTGGACTAGATACTGCCGTTCGTTTCGTCCAAGATTATACAGATGATGAGATTTTAAATAAATTGCATGAAATAGGCAATGAGGATAATACGCTGCTTCAAGGTGGTCTTGAATTGGTAAATAACTATGGACAATCTTCGGACACGAACTCAAGAAAGATCAAGTGTTCTCACTGTTCCTTCTGTGGTCATCCAGGCAGCAAAAGAGCTCATGTTAAGTTCCCTTGTGAATACTGTGATGTGAGTCATGGCGATGGTTGCATCAAAAAGCCGGATGGATTTAAATGCAATTGTTCCTCATGTGATACGGATCGAGAAGAAAAAGAGAAAAAGAAGCAAGAAAATTGGCGATTAAGAGTTTGCAACAAGATTGCCATGGAGCCAAATTTTCCCAGTAGTGAACTTGTTCAAATATACCTGGGCGACAATCATAGTTATTTTAGTGATGATGACCTTATATTATCTTGGGGAAGCCCGAGAACCGAAATGCTGATTGATTTTTTAGTTTTTCATCTACGGTGGGAGCCATCTTACATTCGTCAAAGGATGCTTCCCATGCTATCCACCATTTTTCTCAGAGAAAAGGCTAATAATCCAATTCAAACTTTGTTATACGGCCAGTACGAGTTTGATTCCATACTTAGAGAAAAAATAAGATATGGACATCCGTTTTATGTTGTCAAATGGAAAAAGGCCACCCCTACACTCGGCAATGTTGTAAACGAAGGCTCTTCCGGGGGATTTGGCACTGGACCAGAAGATGCCATTGACGTCGATGAACCAGTGGATTTAACGGATGAATCTGATTCCCCAGAAATTCATATTCAAGACGGGTGCTCCTTTTTGTTGACCGACGAGAATATGGACCTTGTACGTGCTGCATATCCTGAACAGGCTGCCAAATTTTTGCAGGAAATGGAAGTGAAAAGGCAAAAGACACCAGCCACTCCACCTTGCGGCGGGACTAGCGGAAAGTCTGATTCATCGAAGGCGAAAGGTGTCCAGTTAAGTATAACCGAGTTCTATCGTGCAACCAAAACACAACAAGCAACGGATGCAGGAGATTCGACAAATGAGGGCGAGGAGACAAGTACTGGAAAGAGGAAATCATCAAGTTCTACTCTTTCAAAGTCGGTAAGACGTCGCCTTTTGTTCGAGGACTAG

Protein sequence

MGVGGHFWDLLKPHARTEGFDFLRNKRIAVDLSFWIVQHETAIKASARSPHLRLTFFRTITLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPKIEDRISVDRNRKFAKCVKECVELLELFGVPVIEAKGEAEALCAELNRKGFVDACITADSDAFLFGAKCVIKSFHPNSKANIEAALGLNRKHLIAISLLVGNDHDLKGVQGVGLDTAVRFVQDYTDDEILNKLHEIGNEDNTLLQGGLELVNNYGQSSDTNSRKIKCSHCSFCGHPGSKRAHVKFPCEYCDVSHGDGCIKKPDGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAMEPNFPSSELVQIYLGDNHSYFSDDDLILSWGSPRTEMLIDFLVFHLRWEPSYIRQRMLPMLSTIFLREKANNPIQTLLYGQYEFDSILREKIRYGHPFYVVKWKKATPTLGNVVNEGSSGGFGTGPEDAIDVDEPVDLTDESDSPEIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLQEMEVKRQKTPATPPCGGTSGKSDSSKAKGVQLSITEFYRATKTQQATDAGDSTNEGEETSTGKRKSSSSTLSKSVRRRLLFED
Homology
BLAST of Spg023687 vs. NCBI nr
Match: XP_038876412.1 (flap endonuclease GEN-like 1 isoform X2 [Benincasa hispida])

HSP 1 Score: 1097.8 bits (2838), Expect = 0.0e+00
Identity = 546/609 (89.66%), Postives = 573/609 (94.09%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPHARTEGFDFLRNKRIAVDLSFWIVQHETAIKASARSPHLRLTFFRTI 60
           MGVGGHFWDLLKPHARTEGFDFLRNKR+AVDLSFWIVQHETAIK+SARSPHLRLTFFRTI
Sbjct: 1   MGVGGHFWDLLKPHARTEGFDFLRNKRVAVDLSFWIVQHETAIKSSARSPHLRLTFFRTI 60

Query: 61  TLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPKIEDRISVDRNRKFAKCV 120
           TLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPK+EDRISVDRNRKFAKCV
Sbjct: 61  TLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPKVEDRISVDRNRKFAKCV 120

Query: 121 KECVELLELFGVPVIEAKGEAEALCAELNRKGFVDACITADSDAFLFGAKCVIKSFHPNS 180
           KECVELLELFGVPV+EAKGEAEALCAELNRKGFVDACITADSDAFLFGAKCVIKS HPNS
Sbjct: 121 KECVELLELFGVPVLEAKGEAEALCAELNRKGFVDACITADSDAFLFGAKCVIKSIHPNS 180

Query: 181 K--------ANIEAALGLNRKHLIAISLLVGNDHDLKGVQGVGLDTAVRFVQDYTDDEIL 240
           K        ++IEAALGLNRKHLIAISLLVGNDH+L GVQGVGLDTAVRFVQDYTDDEIL
Sbjct: 181 KEPLECYFISDIEAALGLNRKHLIAISLLVGNDHNLNGVQGVGLDTAVRFVQDYTDDEIL 240

Query: 241 NKLHEIGNEDNTLLQGGLELVNNYGQSSDTNSRKIKCSHCSFCGHPGSKRAHVKFPCEYC 300
           NKL+EIGNEDN++LQGGLELVN+YGQS   +SRKIKCSHCSFCGHPGSKRAHVKFPCEYC
Sbjct: 241 NKLYEIGNEDNSMLQGGLELVNDYGQSLKMSSRKIKCSHCSFCGHPGSKRAHVKFPCEYC 300

Query: 301 DVSHGDGCIKKPDGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAMEPNFPSSELVQIY 360
           DVSHG+GCIKKPDGFKCNCSSCDTDREEKEKKKQENWRL+VC KIAMEPNFPSSEL+Q+Y
Sbjct: 301 DVSHGEGCIKKPDGFKCNCSSCDTDREEKEKKKQENWRLKVCKKIAMEPNFPSSELIQMY 360

Query: 361 LGDNHSYFSDDDLILSWGSPRTEMLIDFLVFHLRWEPSYIRQRMLPMLSTIFLREKANNP 420
             DNHSYFSDDDL+LSWGSPRTEML+DFLVFHLRWEPSYIRQRMLPMLSTIFLRE ANNP
Sbjct: 361 QCDNHSYFSDDDLVLSWGSPRTEMLVDFLVFHLRWEPSYIRQRMLPMLSTIFLREMANNP 420

Query: 421 IQTLLYGQYEFDSILREKIRYGHPFYVVKWKKATPTLGNVVNEGSSGGFGTGPEDAIDVD 480
           IQTLLYGQYEFDSILREKIRYGHPFYVVKWKKA PTL NV+ EGSS GFGTGP+DAID+D
Sbjct: 421 IQTLLYGQYEFDSILREKIRYGHPFYVVKWKKAVPTLSNVIYEGSSEGFGTGPDDAIDID 480

Query: 481 EPVDLTDESDSPEIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLQEMEVKRQKTPATPPC 540
           E VDLTDESDSP+IHIQDGCSFLLTDEN+DLV AAYPEQAA FLQE E+KRQKTP TP C
Sbjct: 481 ETVDLTDESDSPKIHIQDGCSFLLTDENIDLVGAAYPEQAANFLQEKEMKRQKTPTTPAC 540

Query: 541 GGTSGKSDSSKAKGVQLSITEFYRATKTQQAT-DAGDSTNEGEETSTGKRK-SSSSTLSK 600
            GTSGKSDSSK KGVQLSITEFYRATKTQQ T +  D TNE EETSTGKRK S+SSTLSK
Sbjct: 541 -GTSGKSDSSKGKGVQLSITEFYRATKTQQQTSETTDLTNEDEETSTGKRKASTSSTLSK 600

BLAST of Spg023687 vs. NCBI nr
Match: XP_038876411.1 (flap endonuclease GEN-like 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 1088.6 bits (2814), Expect = 0.0e+00
Identity = 547/636 (86.01%), Postives = 573/636 (90.09%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPHARTEGFDFLRNKRIAVDLSFWIVQHETAIKASARSPHLRLTFFRTI 60
           MGVGGHFWDLLKPHARTEGFDFLRNKR+AVDLSFWIVQHETAIK+SARSPHLRLTFFRTI
Sbjct: 1   MGVGGHFWDLLKPHARTEGFDFLRNKRVAVDLSFWIVQHETAIKSSARSPHLRLTFFRTI 60

Query: 61  TLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPKIEDRISVDRNRKFAKCV 120
           TLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPK+EDRISVDRNRKFAKCV
Sbjct: 61  TLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPKVEDRISVDRNRKFAKCV 120

Query: 121 KECVELLELFGVPVIEAKGEAEALCAELNRKGFVDACITADSDAFLFGAKCVIKSFHPNS 180
           KECVELLELFGVPV+EAKGEAEALCAELNRKGFVDACITADSDAFLFGAKCVIKS HPNS
Sbjct: 121 KECVELLELFGVPVLEAKGEAEALCAELNRKGFVDACITADSDAFLFGAKCVIKSIHPNS 180

Query: 181 KA-----------------------------------NIEAALGLNRKHLIAISLLVGND 240
           KA                                   +IEAALGLNRKHLIAISLLVGND
Sbjct: 181 KASTPIYTSLLLYVTSFLPCSLEYHHMQEPLECYFISDIEAALGLNRKHLIAISLLVGND 240

Query: 241 HDLKGVQGVGLDTAVRFVQDYTDDEILNKLHEIGNEDNTLLQGGLELVNNYGQSSDTNSR 300
           H+L GVQGVGLDTAVRFVQDYTDDEILNKL+EIGNEDN++LQGGLELVN+YGQS   +SR
Sbjct: 241 HNLNGVQGVGLDTAVRFVQDYTDDEILNKLYEIGNEDNSMLQGGLELVNDYGQSLKMSSR 300

Query: 301 KIKCSHCSFCGHPGSKRAHVKFPCEYCDVSHGDGCIKKPDGFKCNCSSCDTDREEKEKKK 360
           KIKCSHCSFCGHPGSKRAHVKFPCEYCDVSHG+GCIKKPDGFKCNCSSCDTDREEKEKKK
Sbjct: 301 KIKCSHCSFCGHPGSKRAHVKFPCEYCDVSHGEGCIKKPDGFKCNCSSCDTDREEKEKKK 360

Query: 361 QENWRLRVCNKIAMEPNFPSSELVQIYLGDNHSYFSDDDLILSWGSPRTEMLIDFLVFHL 420
           QENWRL+VC KIAMEPNFPSSEL+Q+Y  DNHSYFSDDDL+LSWGSPRTEML+DFLVFHL
Sbjct: 361 QENWRLKVCKKIAMEPNFPSSELIQMYQCDNHSYFSDDDLVLSWGSPRTEMLVDFLVFHL 420

Query: 421 RWEPSYIRQRMLPMLSTIFLREKANNPIQTLLYGQYEFDSILREKIRYGHPFYVVKWKKA 480
           RWEPSYIRQRMLPMLSTIFLRE ANNPIQTLLYGQYEFDSILREKIRYGHPFYVVKWKKA
Sbjct: 421 RWEPSYIRQRMLPMLSTIFLREMANNPIQTLLYGQYEFDSILREKIRYGHPFYVVKWKKA 480

Query: 481 TPTLGNVVNEGSSGGFGTGPEDAIDVDEPVDLTDESDSPEIHIQDGCSFLLTDENMDLVR 540
            PTL NV+ EGSS GFGTGP+DAID+DE VDLTDESDSP+IHIQDGCSFLLTDEN+DLV 
Sbjct: 481 VPTLSNVIYEGSSEGFGTGPDDAIDIDETVDLTDESDSPKIHIQDGCSFLLTDENIDLVG 540

Query: 541 AAYPEQAAKFLQEMEVKRQKTPATPPCGGTSGKSDSSKAKGVQLSITEFYRATKTQQAT- 600
           AAYPEQAA FLQE E+KRQKTP TP C GTSGKSDSSK KGVQLSITEFYRATKTQQ T 
Sbjct: 541 AAYPEQAANFLQEKEMKRQKTPTTPAC-GTSGKSDSSKGKGVQLSITEFYRATKTQQQTS 600

BLAST of Spg023687 vs. NCBI nr
Match: TYK24473.1 (flap endonuclease GEN-like 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1083.6 bits (2801), Expect = 0.0e+00
Identity = 540/609 (88.67%), Postives = 570/609 (93.60%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPHARTEGFDFLRNKRIAVDLSFWIVQHETAIKASARSPHLRLTFFRTI 60
           MGVGGHFWDLLKP+ARTEGFDFLRNKR+AVDLSFWIVQHETAIK+++RSPHLRLTFFRTI
Sbjct: 1   MGVGGHFWDLLKPNARTEGFDFLRNKRVAVDLSFWIVQHETAIKSTSRSPHLRLTFFRTI 60

Query: 61  TLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPKIEDRISVDRNRKFAKCV 120
           TLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPK+EDRISV RNRKFAKCV
Sbjct: 61  TLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPKVEDRISVHRNRKFAKCV 120

Query: 121 KECVELLELFGVPVIEAKGEAEALCAELNRKGFVDACITADSDAFLFGAKCVIKSFHPNS 180
           KECVELLELFGVPV+EAKGEAEALCAELN+KGFVDACITADSDAFLFGAKCVIKS HPNS
Sbjct: 121 KECVELLELFGVPVLEAKGEAEALCAELNQKGFVDACITADSDAFLFGAKCVIKSIHPNS 180

Query: 181 K--------ANIEAALGLNRKHLIAISLLVGNDHDLKGVQGVGLDTAVRFVQDYTDDEIL 240
           K        ++IEAALGLNR HLIAISLLVGNDHDL GVQGVGLDTAVRFVQDYTDD+IL
Sbjct: 181 KEPLECYFMSDIEAALGLNRNHLIAISLLVGNDHDLNGVQGVGLDTAVRFVQDYTDDQIL 240

Query: 241 NKLHEIGNEDNTLLQGGLELVNNYGQSSDTNSRKIKCSHCSFCGHPGSKRAHVKFPCEYC 300
           NKL+EIGNED T LQ G ELVNNYGQSS+T+SRKIKCSHCSFCGHPGSKRAHVKFPCEYC
Sbjct: 241 NKLYEIGNEDTTALQSGFELVNNYGQSSETDSRKIKCSHCSFCGHPGSKRAHVKFPCEYC 300

Query: 301 DVSHGDGCIKKPDGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAMEPNFPSSELVQIY 360
           DVSHG+GCIKKPDGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAMEPNFPSSEL+Q+Y
Sbjct: 301 DVSHGEGCIKKPDGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAMEPNFPSSELIQMY 360

Query: 361 LGDNHSYFSDDDLILSWGSPRTEMLIDFLVFHLRWEPSYIRQRMLPMLSTIFLREKANNP 420
             DNHSYFSDDDL+LSWGSPRTE+L+DFLVFHL+WEPSYIRQRMLPMLSTIFLRE ANNP
Sbjct: 361 QCDNHSYFSDDDLVLSWGSPRTELLVDFLVFHLQWEPSYIRQRMLPMLSTIFLREMANNP 420

Query: 421 IQTLLYGQYEFDSILREKIRYGHPFYVVKWKKATPTLGNVVNEGSSGGFGTGPEDAIDVD 480
           IQTLL+GQYEFDSILREKIR+GH FYVVKWKKA P + NV+ E S G FGTGPEDAIDVD
Sbjct: 421 IQTLLFGQYEFDSILREKIRFGHSFYVVKWKKAVPAISNVMYEDSLGEFGTGPEDAIDVD 480

Query: 481 EPVDLTDESDSPEIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLQEMEVKRQKTPATPPC 540
           E VDLTDE DSP+IHIQDGCSFLLTDENM+LV AAYPEQAAKFLQE E+KRQKTP+TP C
Sbjct: 481 EAVDLTDEFDSPKIHIQDGCSFLLTDENMELVGAAYPEQAAKFLQEKEMKRQKTPSTPAC 540

Query: 541 GGTSGKSDSSKAKGVQLSITEFYRATKTQQAT-DAGDSTNEGEETSTGKRK-SSSSTLSK 600
            GTSGKSDSSKAKGVQLSITEFYRATKTQQ T D  DSTNE EETSTGKRK SSSSTLSK
Sbjct: 541 -GTSGKSDSSKAKGVQLSITEFYRATKTQQQTADTTDSTNEDEETSTGKRKASSSSTLSK 600

BLAST of Spg023687 vs. NCBI nr
Match: XP_008460162.1 (PREDICTED: flap endonuclease GEN-like 1 [Cucumis melo])

HSP 1 Score: 1082.4 bits (2798), Expect = 0.0e+00
Identity = 539/609 (88.51%), Postives = 570/609 (93.60%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPHARTEGFDFLRNKRIAVDLSFWIVQHETAIKASARSPHLRLTFFRTI 60
           MGVGGHFWDLLKP+ARTEGFDFLRNKR+AVDLSFWIVQHETAIK+++RSPHLRLTFFRTI
Sbjct: 1   MGVGGHFWDLLKPNARTEGFDFLRNKRVAVDLSFWIVQHETAIKSTSRSPHLRLTFFRTI 60

Query: 61  TLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPKIEDRISVDRNRKFAKCV 120
           TLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPK+EDRISV RNRKFAKCV
Sbjct: 61  TLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPKVEDRISVHRNRKFAKCV 120

Query: 121 KECVELLELFGVPVIEAKGEAEALCAELNRKGFVDACITADSDAFLFGAKCVIKSFHPNS 180
           KECVELLELFGVPV+EAKGEAEALCAELN+KGFVDACITADSDAFLFGAKCVIKS HPNS
Sbjct: 121 KECVELLELFGVPVLEAKGEAEALCAELNQKGFVDACITADSDAFLFGAKCVIKSIHPNS 180

Query: 181 K--------ANIEAALGLNRKHLIAISLLVGNDHDLKGVQGVGLDTAVRFVQDYTDDEIL 240
           K        ++IEAALGLNR HLIAISLLVGNDHDL GVQGVGLDTAVRFVQDYTDD+IL
Sbjct: 181 KEPLECYFMSDIEAALGLNRNHLIAISLLVGNDHDLNGVQGVGLDTAVRFVQDYTDDQIL 240

Query: 241 NKLHEIGNEDNTLLQGGLELVNNYGQSSDTNSRKIKCSHCSFCGHPGSKRAHVKFPCEYC 300
           NKL+EIGNED T LQ G ELVNNYGQSS+T+SRKIKCSHCSFCGHPGSKRAHVKFPCEYC
Sbjct: 241 NKLYEIGNEDTTALQSGFELVNNYGQSSETDSRKIKCSHCSFCGHPGSKRAHVKFPCEYC 300

Query: 301 DVSHGDGCIKKPDGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAMEPNFPSSELVQIY 360
           DVSHG+GCIKKPDGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAMEPNFPSSEL+Q+Y
Sbjct: 301 DVSHGEGCIKKPDGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAMEPNFPSSELIQMY 360

Query: 361 LGDNHSYFSDDDLILSWGSPRTEMLIDFLVFHLRWEPSYIRQRMLPMLSTIFLREKANNP 420
             DNHSYFSDDDL+LSWGSPRTE+L+DFLVFHL+WEPSYIRQRMLPMLSTIFLRE ANNP
Sbjct: 361 QCDNHSYFSDDDLVLSWGSPRTELLVDFLVFHLQWEPSYIRQRMLPMLSTIFLREMANNP 420

Query: 421 IQTLLYGQYEFDSILREKIRYGHPFYVVKWKKATPTLGNVVNEGSSGGFGTGPEDAIDVD 480
           IQTLL+GQYEFDSILREKIR+GH FYVVKWKKA P + NV+ E S G FGTGPEDAIDVD
Sbjct: 421 IQTLLFGQYEFDSILREKIRFGHSFYVVKWKKAVPAISNVMYEDSLGEFGTGPEDAIDVD 480

Query: 481 EPVDLTDESDSPEIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLQEMEVKRQKTPATPPC 540
           E VDLTDE DSP+IHIQDGCSFLLTDENM+LV AAYPEQAAKFLQE E+KRQKTP+TP C
Sbjct: 481 EAVDLTDEFDSPKIHIQDGCSFLLTDENMELVGAAYPEQAAKFLQEKEMKRQKTPSTPAC 540

Query: 541 GGTSGKSDSSKAKGVQLSITEFYRATKTQQAT-DAGDSTNEGEETSTGKRK-SSSSTLSK 600
            GTSGKSDSSKAKGVQLSITEFYRATKTQQ T D  DSTNE EETSTGKR+ SSSSTLSK
Sbjct: 541 -GTSGKSDSSKAKGVQLSITEFYRATKTQQQTADTTDSTNEDEETSTGKRRASSSSTLSK 600

BLAST of Spg023687 vs. NCBI nr
Match: KAA0040028.1 (flap endonuclease GEN-like 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1073.9 bits (2776), Expect = 4.6e-310
Identity = 541/637 (84.93%), Postives = 570/637 (89.48%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPHARTEGFDFLRNKRIAVDLSFWIVQHETAIKASARSPHLRLTFFRTI 60
           MGVGGHFWDLLKP+ARTEGFDFLRNKR+AVDLSFWIVQHETAIK+++RSPHLRLTFFRTI
Sbjct: 1   MGVGGHFWDLLKPNARTEGFDFLRNKRVAVDLSFWIVQHETAIKSTSRSPHLRLTFFRTI 60

Query: 61  TLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPKIEDRISVDRNRKFAKCV 120
           TLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPK+EDRISV RNRKFAKCV
Sbjct: 61  TLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPKVEDRISVHRNRKFAKCV 120

Query: 121 KECVELLELFGVPVIEAKGEAEALCAELNRKGFVDACITADSDAFLFGAKCVIKSFHPNS 180
           KECVELLELFGVPV+EAKGEAEALCAELN+KGFVDACITADSDAFLFGAKCVIKS HPNS
Sbjct: 121 KECVELLELFGVPVLEAKGEAEALCAELNQKGFVDACITADSDAFLFGAKCVIKSIHPNS 180

Query: 181 KA------------------------------------NIEAALGLNRKHLIAISLLVGN 240
           KA                                    +IEAALGLNR HLIAISLLVGN
Sbjct: 181 KACTPIYTSPLLQVTSLILPCSLEYHHMQEPLECYFMSDIEAALGLNRNHLIAISLLVGN 240

Query: 241 DHDLKGVQGVGLDTAVRFVQDYTDDEILNKLHEIGNEDNTLLQGGLELVNNYGQSSDTNS 300
           DHDL GVQGVGLDTAVRFVQDYTDD+ILNKL+EIGNED T LQ G ELVNNYGQSS+T+S
Sbjct: 241 DHDLNGVQGVGLDTAVRFVQDYTDDQILNKLYEIGNEDTTALQSGFELVNNYGQSSETDS 300

Query: 301 RKIKCSHCSFCGHPGSKRAHVKFPCEYCDVSHGDGCIKKPDGFKCNCSSCDTDREEKEKK 360
           RKIKCSHCSFCGHPGSKRAHVKFPCEYCDVSHG+GCIKKPDGFKCNCSSCDTDREEKEKK
Sbjct: 301 RKIKCSHCSFCGHPGSKRAHVKFPCEYCDVSHGEGCIKKPDGFKCNCSSCDTDREEKEKK 360

Query: 361 KQENWRLRVCNKIAMEPNFPSSELVQIYLGDNHSYFSDDDLILSWGSPRTEMLIDFLVFH 420
           KQENWRLRVCNKIAMEPNFPSSEL+Q+Y  DNHSYFSDDDL+LSWGSPRTE+L+DFLVFH
Sbjct: 361 KQENWRLRVCNKIAMEPNFPSSELIQMYQCDNHSYFSDDDLVLSWGSPRTELLVDFLVFH 420

Query: 421 LRWEPSYIRQRMLPMLSTIFLREKANNPIQTLLYGQYEFDSILREKIRYGHPFYVVKWKK 480
           L+WEPSYIRQRMLPMLSTIFLRE ANNPIQTLL+GQYEFDSILREKIR+GH FYVVKWKK
Sbjct: 421 LQWEPSYIRQRMLPMLSTIFLREMANNPIQTLLFGQYEFDSILREKIRFGHSFYVVKWKK 480

Query: 481 ATPTLGNVVNEGSSGGFGTGPEDAIDVDEPVDLTDESDSPEIHIQDGCSFLLTDENMDLV 540
           A P + NV+ E S G FGTGPEDAIDVDE VDLTDE DSP+IHIQDGCSFLLTDENM+LV
Sbjct: 481 AVPAISNVMYEDSLGEFGTGPEDAIDVDEAVDLTDEFDSPKIHIQDGCSFLLTDENMELV 540

Query: 541 RAAYPEQAAKFLQEMEVKRQKTPATPPCGGTSGKSDSSKAKGVQLSITEFYRATKTQQAT 600
            AAYPEQAAKFLQE E+KRQKTP+TP C GTSGKSDSSKAKGVQLSITEFYRATKTQQ T
Sbjct: 541 GAAYPEQAAKFLQEKEMKRQKTPSTPAC-GTSGKSDSSKAKGVQLSITEFYRATKTQQQT 600

BLAST of Spg023687 vs. ExPASy Swiss-Prot
Match: Q9LPD2 (Flap endonuclease GEN-like 1 OS=Arabidopsis thaliana OX=3702 GN=GEN1 PE=1 SV=3)

HSP 1 Score: 597.8 bits (1540), Expect = 1.3e-169
Identity = 317/614 (51.63%), Postives = 423/614 (68.89%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPHARTEGFDFLRNKRIAVDLSFWIVQHETAIKASARSPHLRLTFFRTI 60
           MGVGG+FWDLL+P+A+ +GFDFLRNKR+AVDLSFWIVQHETA+K     PHLRLTFFRTI
Sbjct: 1   MGVGGNFWDLLRPYAQQQGFDFLRNKRVAVDLSFWIVQHETAVKGFVLKPHLRLTFFRTI 60

Query: 61  TLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPKIEDRISVDRNRKFAKCV 120
            LF+KFGA+PVFVVDGTPSPLKS+ARI+RFFR SGIDT +LP I+D +SV+RN+ F++ V
Sbjct: 61  NLFSKFGAYPVFVVDGTPSPLKSQARISRFFRSSGIDTCNLPVIKDGVSVERNKLFSEWV 120

Query: 121 KECVELLELFGVPVIEAKGEAEALCAELNRKGFVDACITADSDAFLFGAKCVIKSFHPNS 180
           +ECVELLEL G+PV++A GEAEALCA+LN +GFVDACIT DSDAFLFGA CVIK   PNS
Sbjct: 121 RECVELLELLGIPVLKANGEAEALCAQLNSQGFVDACITPDSDAFLFGAMCVIKDIKPNS 180

Query: 181 K--------ANIEAALGLNRKHLIAISLLVGNDHDLKGVQGVGLDTAVRFVQDYTDDEIL 240
           +        ++IE+ LGL RKHLIAISLLVGND+D  GV G+G+D A+R V+++++D++L
Sbjct: 181 REPFECYHMSHIESGLGLKRKHLIAISLLVGNDYDSGGVLGIGVDKALRIVREFSEDQVL 240

Query: 241 NKLHEIGNEDNTLLQGGLELVNNYGQSSDTNSRKIKCSHCSFCGHPGSKRAHVKFPCEYC 300
            +L +IGN     + GG++  ++ G+   +  +K +  HCS CGH GSKR H K  CE+C
Sbjct: 241 ERLQDIGNGLQPAVPGGIKSGDD-GEEFRSEMKK-RSPHCSRCGHLGSKRTHFKSSCEHC 300

Query: 301 DVSHGDGCIKKPDGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAMEPNFPSSELVQIY 360
                 GCIKKP GF+C CS C  DR+ +E+KK  +W ++VC+KIA+ P FP+ +++++Y
Sbjct: 301 GCD--SGCIKKPLGFRCECSFCSKDRDLREQKKTNDWWIKVCDKIALAPEFPNRKIIELY 360

Query: 361 LGDNHSYFSDDDLILSWGSPRTEMLIDFLVFHLRWEPSYIRQRMLPMLSTIFLREKA-NN 420
           L D     + D   +SWG+P T ML+D +VF L W+PSY+R+ +LPMLSTI+LREKA NN
Sbjct: 361 LSD--GLMTGDGSSMSWGTPDTGMLVDLMVFKLHWDPSYVRKMLLPMLSTIYLREKARNN 420

Query: 421 PIQTLLYGQYEFDSILREKIRYGHPFYVVKWKKATPTLGNVVNEGSSGGFGTGPEDAIDV 480
               LL  QYEF SI   K RYGH  +V++W+K   T G   +          PE++I V
Sbjct: 421 TGYALLCDQYEFHSIKCIKTRYGHQSFVIRWRKPKSTSGYSHSHSE-------PEESIVV 480

Query: 481 ----DEPVDLTDESDSPEIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLQEMEVKRQKTP 540
               +E VD  D  + P++   +G  FLLTDE + LV++A+P++   FL E +++  K  
Sbjct: 481 LEEEEESVDPLDGLNEPQVQNDNGDCFLLTDECIGLVQSAFPDETEHFLHEKKLRESKKK 540

Query: 541 ATPPCGGTSGKSDSSKAKGVQLSITEFYRATK---TQQATDAGDSTNEGEETSTGKRKSS 599
                      +  +   GVQ SIT+FYR+ K     Q+ + G S+    E       +S
Sbjct: 541 NV---SEEETATPRATTMGVQRSITDFYRSAKKAAAGQSIETGGSSKASAEKKRQATSTS 598

BLAST of Spg023687 vs. ExPASy Swiss-Prot
Match: Q64MA3 (Flap endonuclease GEN-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN=RAD PE=1 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 3.4e-157
Identity = 308/639 (48.20%), Postives = 409/639 (64.01%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPHARTEGFDFLRNKRIAVDLSFWIVQHETAIKA---SARSPHLRLTFF 60
           MGVGG FWDLLKP+AR EG  +LR +R+AVDLSFW+V H  AI+A    AR PHLR  FF
Sbjct: 1   MGVGGSFWDLLKPYARHEGAGYLRGRRVAVDLSFWVVSHSAAIRARSPHARLPHLRTLFF 60

Query: 61  RTITLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPKIEDRISVD-----R 120
           RT++LF+K GAFPVFVVDG PSPLKS+ R ARFFR SG+D + LP  E   S D     R
Sbjct: 61  RTLSLFSKMGAFPVFVVDGQPSPLKSQVRAARFFRGSGMDLAALPSTEAEASADALVQPR 120

Query: 121 NRKFAKCVKECVELLELFGVPVIEAKGEAEALCAELNRKGFVDACITADSDAFLFGAKCV 180
           N KF + V++CVELLE  G+PV+ AKGE EALCA+LN +G VDACIT+DSDAFLFGAK V
Sbjct: 121 NAKFTRYVEDCVELLEYLGMPVLRAKGEGEALCAQLNNQGHVDACITSDSDAFLFGAKTV 180

Query: 181 IKSFHPNSK--------ANIEAALGLNRKHLIAISLLVGNDHDLKGVQGVGLDTAVRFVQ 240
           IK    N K        A+IE+ LGL RK ++A++LLVG+DHDL GV G G +TA+RFVQ
Sbjct: 181 IKVLRSNCKEPFECYNMADIESGLGLKRKQMVAMALLVGSDHDLHGVPGFGPETALRFVQ 240

Query: 241 DYTDDEILNKLHEIGNEDNTLLQGGLELVNNYGQSSDTNSRKIKCSHCSFCGHPGSKRAH 300
            + +D +L KL+EIG      +      +++    S  +  + +  HCS CGHPG+K+ H
Sbjct: 241 LFDEDNVLAKLYEIGKGVYPFIGVSAPNIDDLPSPSTKSLPRARSPHCSHCGHPGNKKNH 300

Query: 301 VKFPCEYCDVSHGDGCIKKPDGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAMEPNFP 360
           +K  C +C V   + C++KP GF C C SCD  R+ K +++ ENW+++VC +IA E NFP
Sbjct: 301 IKDGCNFCLVDSLENCVEKPAGFICECPSCDKARDLKVQRRNENWQIKVCKRIAAETNFP 360

Query: 361 SSELVQIYLGDNHSYFSDDDLILSWGSPRTEMLIDFLVFHLRWEPSYIRQRMLPMLSTIF 420
           + E++ +YL D++    +   +L+W  P  E+L+DFL F   WEP+YIRQRMLPMLSTI+
Sbjct: 361 NEEIINLYLNDDNLDNENGVPLLTWNKPDMEILVDFLSFKQNWEPAYIRQRMLPMLSTIY 420

Query: 421 LREKANNPIQT-LLYGQYEFDSILREKIRYGHPFYVVKWKKAT-------PTLGNVVNEG 480
           LRE A++  ++ LLY QY+F SI R KIRYGHP+Y+VKWK+ T       P       EG
Sbjct: 421 LREMASSQSKSFLLYDQYKFHSIQRIKIRYGHPYYLVKWKRVTRSMISNDPPSKQTELEG 480

Query: 481 SSGGFGT--GPEDAID--------VDEPVDLTDESDSPEIHIQDGCSFLLTDENMDLVRA 540
            +       G ++ +D        + E  +L DE D P++   D   FLLTDE+++LV A
Sbjct: 481 KNDKVEVLDGDDEVVDEEEEEPTMISETTELLDEPDVPQVLDDDKDCFLLTDEDIELVNA 540

Query: 541 AYPEQAAKFLQEMEVKRQKTPATPPCGGTSG--KSDSSKAKGVQLSITEFYRATKTQQAT 600
           A+P++A +F +E  +K  K+ A       +G       +  GVQLSI EFYR+ K     
Sbjct: 541 AFPDEAQRFQEEQRLKEAKSIARKSKLNVAGFETPKGPRPSGVQLSIKEFYRSKK----- 600

BLAST of Spg023687 vs. ExPASy Swiss-Prot
Match: Q8BMI4 (Flap endonuclease GEN homolog 1 OS=Mus musculus OX=10090 GN=Gen1 PE=1 SV=2)

HSP 1 Score: 182.6 bits (462), Expect = 1.3e-44
Identity = 154/559 (27.55%), Postives = 244/559 (43.65%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPHARTEGFDFLRNKRIAVDLSFWIVQHETAIK--ASARSPHLRLTFFR 60
           MGV    W +L+P  +      L  K IAVDLS W+ + +T  K   + + PHLR  FFR
Sbjct: 1   MGV-NDLWQILEPVKQHIHLQDLSGKTIAVDLSLWVCEAQTVKKMIGTVKKPHLRNLFFR 60

Query: 61  TITLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPKIEDRISVDRNRKFAK 120
            I+   +     VFV++G P  LK+     R        T   P  + R        F  
Sbjct: 61  -ISYLTQMNVKLVFVMEGEPPMLKADVISKR------TQTRYGPSGKSRSQKTGRSHFKS 120

Query: 121 CVKECVELLELFGVPVIEAKGEAEALCAELNRKGFVDACITADSDAFLFGAKCVIKSFHP 180
            ++EC+E+LE  G+P ++A GEAEA+CA LN  G VD C+T D DAFL+GA+ V ++F  
Sbjct: 121 VLRECLEMLECLGMPWVQAAGEAEAMCAYLNASGHVDGCLTNDGDAFLYGAQTVYRNFTM 180

Query: 181 NSK---------ANIEAALGLNRKHLIAISLLVGNDHDLKGVQGVGLDTAVRFVQDYTDD 240
           N+K         ++I++ LGL+R  L+ +++L+G D+  KGV GVG + A++ +Q +   
Sbjct: 181 NTKDPHVDCYTISSIKSKLGLDRDALVGLAVLLGCDYLPKGVPGVGKEQALKLLQIFKGQ 240

Query: 241 EILNKLHEIGNEDNTLLQGGLELVNNYGQSSDTNSRKIKCSHCSFCGHPGSKRAHVKFPC 300
            +L + ++   ED                 S   S   K  HCS C HPGS + H +  C
Sbjct: 241 SLLQRFNQ-WIEDPCY--------------SVPQSAPKKVVHCSVCSHPGSPKDHERNGC 300

Query: 301 EYCDVSHGDGCIKKPDGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAMEPNFPSSELV 360
             C       C      + C C    TD      + + N + + C+       FP  E++
Sbjct: 301 ILC--KSDKYCEPHDYDYLCPCEWHQTDHNRHLSEIENNIKKKACSC----EGFPFHEVI 360

Query: 361 QIYLGDNHSYFSDDDLILSWGSPRTEMLIDFLVFHLRWEPSYIRQRMLPMLSTIFLRE-- 420
           Q +L + +         +++  P   +   F V  + W   Y  +++L +L+   + E  
Sbjct: 361 QEFLLNKNKMLKP----ITYQRPDLLLFQRFTVQKMEWPSHYACEKLLVLLTRYDMIERK 420

Query: 421 ---KANNPIQTLLYGQYEFDSILREKIRYGHPFYVVKWKKATPTLGNVVNEGSSGGFGTG 480
              K +N +Q +         I++ ++R G     ++W+K       VV +G  G     
Sbjct: 421 HGRKTSNQLQPI--------RIVKPRVRNGVHCLEIEWEKPE---HYVVEDGDPGKLS-- 480

Query: 481 PEDAIDVDEPVDLTDESDSPEIHIQDGCSFLLTDENMDLVRAAYPEQAA---KFLQEMEV 540
                                         LLT E   L  AAYP+  A   K L E + 
Sbjct: 481 ------------------------------LLTMEEASLFEAAYPDAVAVYQKQLSETKG 483

BLAST of Spg023687 vs. ExPASy Swiss-Prot
Match: Q17RS7 (Flap endonuclease GEN homolog 1 OS=Homo sapiens OX=9606 GN=GEN1 PE=1 SV=2)

HSP 1 Score: 173.3 bits (438), Expect = 8.2e-42
Identity = 150/549 (27.32%), Postives = 243/549 (44.26%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPHARTEGFDFLRNKRIAVDLSFWIVQHETAIK--ASARSPHLRLTFFR 60
           MGV    W +L+P  +      L  K IAVDLS W+ + +T  K   S   PHLR  FFR
Sbjct: 1   MGV-NDLWQILEPVKQHIPLRNLGGKTIAVDLSLWVCEAQTVKKMMGSVMKPHLRNLFFR 60

Query: 61  TITLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPKIEDRISVDRNRKFAK 120
            I+   +     VFV++G P  LK+     R     G       +   R        F  
Sbjct: 61  -ISYLTQMDVKLVFVMEGEPPKLKADVISKRNQSRYGSSGKSWSQKTGR------SHFKS 120

Query: 121 CVKECVELLELFGVPVIEAKGEAEALCAELNRKGFVDACITADSDAFLFGAKCVIKSFHP 180
            ++EC+ +LE  G+P ++A GEAEA+CA LN  G VD C+T D D FL+GA+ V ++F  
Sbjct: 121 VLRECLHMLECLGIPWVQAAGEAEAMCAYLNAGGHVDGCLTNDGDTFLYGAQTVYRNFTM 180

Query: 181 NSK---------ANIEAALGLNRKHLIAISLLVGNDHDLKGVQGVGLDTAVRFVQDYTDD 240
           N+K         ++I++ LGL+R  L+ +++L+G D+  KGV GVG + A++ +Q     
Sbjct: 181 NTKDPHVDCYTMSSIKSKLGLDRDALVGLAILLGCDYLPKGVPGVGKEQALKLIQILKGQ 240

Query: 241 EILNKLHEIGNEDNTLLQGGLELVNNYGQSSDTNSRKI----KCSHCSFCGHPGSKRAHV 300
            +L +                   N + ++S  +S ++    K +HCS C HPGS + H 
Sbjct: 241 SLLQRF------------------NRWNETSCNSSPQLLVTKKLAHCSVCSHPGSPKDHE 300

Query: 301 KFPCEYCDVSHGDGCIKKPDGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAMEPNFPS 360
           +  C  C       C      + C C    T+ + +  + + N + + C        FP 
Sbjct: 301 RNGCRLC--KSDKYCEPHDYEYCCPCEWHRTEHDRQLSEVENNIKKKAC----CCEGFPF 360

Query: 361 SELVQIYLGDNHSYFSDDDLILSWGSPRTEMLI--DFLVFHLRWEPSYIRQRMLPMLSTI 420
            E++Q +L       + D L+      R ++L+   F +  + W   Y  +++L +L+  
Sbjct: 361 HEVIQEFL------LNKDKLVKVIRYQRPDLLLFQRFTLEKMEWPNHYACEKLLVLLTHY 420

Query: 421 FLREKANNPIQTLLYGQYEFDSILREKIRYGHPFYVVKWKKATPTLGNVVNEGSSGGFGT 480
            + E+    + +    Q +   I++ +IR G   + ++W+K                   
Sbjct: 421 DMIER---KLGSRNSNQLQPIRIVKTRIRNGVHCFEIEWEK------------------- 473

Query: 481 GPEDAIDVDEPVDLTDESDSPEIHIQDGCSFLLTDENMDLVRAAYPEQAAKF-LQEMEVK 532
            PE     D+               Q G   LLT E   L  AAYPE  A +  Q++E+K
Sbjct: 481 -PEHYAMEDK---------------QHGEFALLTIEEESLFEAAYPEIVAVYQKQKLEIK 473

BLAST of Spg023687 vs. ExPASy Swiss-Prot
Match: Q9VRJ0 (Flap endonuclease GEN OS=Drosophila melanogaster OX=7227 GN=Gen PE=1 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 1.9e-35
Identity = 131/549 (23.86%), Postives = 235/549 (42.81%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPHARTEGFDFLRNKRIAVDLSFWIVQHETAIKASAR-SPHLRLTFFRT 60
           MGV    W +L PH   +  + LR K++A+DL+ W+ +    +        HL+  FFRT
Sbjct: 1   MGV-KELWGVLTPHCERKPINELRGKKVAIDLAGWVCESLNVVDYFVHPRHHLKNLFFRT 60

Query: 61  ITLFAKFGAFPVFVVDGTPSPLKSKARIAR-FFRLSGIDTSDLPKI--EDRISVDRNR-K 120
             L  +    PVFV++G    LKS+    R   +  G+   + P+         D+ R +
Sbjct: 61  CYLIWE-QVTPVFVLEGVAPKLKSQVIAKRNELQFRGVKPKNSPECTQSQPSKGDKGRSR 120

Query: 121 FAKCVKECVELLELFGVPVIEAKGEAEALCAELNRKGFVDACITADSDAFLFGAKCVIKS 180
           F   +K+C  LL   G+  ++  GEAEA CA LN+ G VD  I+ DSD F +GA  V ++
Sbjct: 121 FNHVLKQCETLLLSMGIQCVQGPGEAEAYCAFLNKHGLVDGVISQDSDCFAYGAVRVYRN 180

Query: 181 FHPNSKA---------------NIEAALGLNRKHLIAISLLVGNDHDLKGVQGVGLDTAV 240
           F  +++                 I + +   ++ +I ++LL G D+   G+ G+G D  +
Sbjct: 181 FSVSTQGAQAAAGGAVDIYDMREITSRMDFGQQKIIVMALLCGCDYCPDGIGGIGKDGVL 240

Query: 241 RFVQDYTDDEILNKLHEIGNEDNTLLQGGLELVNNYGQSSDTNSRKIKC---SHCSFCGH 300
           +    Y + EIL+++                  +  G++   N+ +I+    S CS CGH
Sbjct: 241 KLFNKYKETEILDRMR-----------------SWRGETDKYNALEIRVDDKSICSNCGH 300

Query: 301 PGSKRAHVKFPCEYCDVSHGDGCIKKPDGFKCNCSSCDTDREEKEKKKQENWRLRVCNKI 360
            G  ++H K  C  C    G                CD +   KE++      L +  K 
Sbjct: 301 IGKTQSHTKSGCSVCRTHKG----------------CD-ESLWKEQRLSIKSELTLRRKA 360

Query: 361 AMEPNFPSSELVQIYLGDNHSYFSDDDLILSWGSPRTEMLIDFLVFHLRWEPSYIRQRML 420
            + P+FP+ E++  +L +  +     +L L+W  P     I  +   L+W   Y  Q+  
Sbjct: 361 LLSPDFPNEEIIAEFLSEPDTI---PNLNLNWRQPNLVKFIKQIGHLLQWPEIYCFQKFF 420

Query: 421 PMLSTIFLREKANNPIQTLLYGQYEFDSILREKIRYGHPFYVVKWKKATPTLGNVVNEGS 480
           P+L+   +++      + +L   +E   I++++   G P   ++W   +     ++ +  
Sbjct: 421 PILTRWQVQQSKQ---EKILIQPHE---IIKKRTVKGVPSLELRWHDPSGIFKGLIPDKQ 480

Query: 481 SGGFGTGPEDAIDVDEPVDLTDESDSPEIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLQ 527
              +        + + P  + +                 T E +D++  AYP+  A FL+
Sbjct: 481 IAEY--------EAEHPKGIEE--------------LYYTIEPLDMLETAYPDLVAAFLK 482

BLAST of Spg023687 vs. ExPASy TrEMBL
Match: A0A5D3DLF9 (Flap endonuclease GEN-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00140 PE=4 SV=1)

HSP 1 Score: 1083.6 bits (2801), Expect = 0.0e+00
Identity = 540/609 (88.67%), Postives = 570/609 (93.60%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPHARTEGFDFLRNKRIAVDLSFWIVQHETAIKASARSPHLRLTFFRTI 60
           MGVGGHFWDLLKP+ARTEGFDFLRNKR+AVDLSFWIVQHETAIK+++RSPHLRLTFFRTI
Sbjct: 1   MGVGGHFWDLLKPNARTEGFDFLRNKRVAVDLSFWIVQHETAIKSTSRSPHLRLTFFRTI 60

Query: 61  TLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPKIEDRISVDRNRKFAKCV 120
           TLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPK+EDRISV RNRKFAKCV
Sbjct: 61  TLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPKVEDRISVHRNRKFAKCV 120

Query: 121 KECVELLELFGVPVIEAKGEAEALCAELNRKGFVDACITADSDAFLFGAKCVIKSFHPNS 180
           KECVELLELFGVPV+EAKGEAEALCAELN+KGFVDACITADSDAFLFGAKCVIKS HPNS
Sbjct: 121 KECVELLELFGVPVLEAKGEAEALCAELNQKGFVDACITADSDAFLFGAKCVIKSIHPNS 180

Query: 181 K--------ANIEAALGLNRKHLIAISLLVGNDHDLKGVQGVGLDTAVRFVQDYTDDEIL 240
           K        ++IEAALGLNR HLIAISLLVGNDHDL GVQGVGLDTAVRFVQDYTDD+IL
Sbjct: 181 KEPLECYFMSDIEAALGLNRNHLIAISLLVGNDHDLNGVQGVGLDTAVRFVQDYTDDQIL 240

Query: 241 NKLHEIGNEDNTLLQGGLELVNNYGQSSDTNSRKIKCSHCSFCGHPGSKRAHVKFPCEYC 300
           NKL+EIGNED T LQ G ELVNNYGQSS+T+SRKIKCSHCSFCGHPGSKRAHVKFPCEYC
Sbjct: 241 NKLYEIGNEDTTALQSGFELVNNYGQSSETDSRKIKCSHCSFCGHPGSKRAHVKFPCEYC 300

Query: 301 DVSHGDGCIKKPDGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAMEPNFPSSELVQIY 360
           DVSHG+GCIKKPDGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAMEPNFPSSEL+Q+Y
Sbjct: 301 DVSHGEGCIKKPDGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAMEPNFPSSELIQMY 360

Query: 361 LGDNHSYFSDDDLILSWGSPRTEMLIDFLVFHLRWEPSYIRQRMLPMLSTIFLREKANNP 420
             DNHSYFSDDDL+LSWGSPRTE+L+DFLVFHL+WEPSYIRQRMLPMLSTIFLRE ANNP
Sbjct: 361 QCDNHSYFSDDDLVLSWGSPRTELLVDFLVFHLQWEPSYIRQRMLPMLSTIFLREMANNP 420

Query: 421 IQTLLYGQYEFDSILREKIRYGHPFYVVKWKKATPTLGNVVNEGSSGGFGTGPEDAIDVD 480
           IQTLL+GQYEFDSILREKIR+GH FYVVKWKKA P + NV+ E S G FGTGPEDAIDVD
Sbjct: 421 IQTLLFGQYEFDSILREKIRFGHSFYVVKWKKAVPAISNVMYEDSLGEFGTGPEDAIDVD 480

Query: 481 EPVDLTDESDSPEIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLQEMEVKRQKTPATPPC 540
           E VDLTDE DSP+IHIQDGCSFLLTDENM+LV AAYPEQAAKFLQE E+KRQKTP+TP C
Sbjct: 481 EAVDLTDEFDSPKIHIQDGCSFLLTDENMELVGAAYPEQAAKFLQEKEMKRQKTPSTPAC 540

Query: 541 GGTSGKSDSSKAKGVQLSITEFYRATKTQQAT-DAGDSTNEGEETSTGKRK-SSSSTLSK 600
            GTSGKSDSSKAKGVQLSITEFYRATKTQQ T D  DSTNE EETSTGKRK SSSSTLSK
Sbjct: 541 -GTSGKSDSSKAKGVQLSITEFYRATKTQQQTADTTDSTNEDEETSTGKRKASSSSTLSK 600

BLAST of Spg023687 vs. ExPASy TrEMBL
Match: A0A1S3CD60 (flap endonuclease GEN-like 1 OS=Cucumis melo OX=3656 GN=LOC103499055 PE=4 SV=1)

HSP 1 Score: 1082.4 bits (2798), Expect = 0.0e+00
Identity = 539/609 (88.51%), Postives = 570/609 (93.60%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPHARTEGFDFLRNKRIAVDLSFWIVQHETAIKASARSPHLRLTFFRTI 60
           MGVGGHFWDLLKP+ARTEGFDFLRNKR+AVDLSFWIVQHETAIK+++RSPHLRLTFFRTI
Sbjct: 1   MGVGGHFWDLLKPNARTEGFDFLRNKRVAVDLSFWIVQHETAIKSTSRSPHLRLTFFRTI 60

Query: 61  TLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPKIEDRISVDRNRKFAKCV 120
           TLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPK+EDRISV RNRKFAKCV
Sbjct: 61  TLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPKVEDRISVHRNRKFAKCV 120

Query: 121 KECVELLELFGVPVIEAKGEAEALCAELNRKGFVDACITADSDAFLFGAKCVIKSFHPNS 180
           KECVELLELFGVPV+EAKGEAEALCAELN+KGFVDACITADSDAFLFGAKCVIKS HPNS
Sbjct: 121 KECVELLELFGVPVLEAKGEAEALCAELNQKGFVDACITADSDAFLFGAKCVIKSIHPNS 180

Query: 181 K--------ANIEAALGLNRKHLIAISLLVGNDHDLKGVQGVGLDTAVRFVQDYTDDEIL 240
           K        ++IEAALGLNR HLIAISLLVGNDHDL GVQGVGLDTAVRFVQDYTDD+IL
Sbjct: 181 KEPLECYFMSDIEAALGLNRNHLIAISLLVGNDHDLNGVQGVGLDTAVRFVQDYTDDQIL 240

Query: 241 NKLHEIGNEDNTLLQGGLELVNNYGQSSDTNSRKIKCSHCSFCGHPGSKRAHVKFPCEYC 300
           NKL+EIGNED T LQ G ELVNNYGQSS+T+SRKIKCSHCSFCGHPGSKRAHVKFPCEYC
Sbjct: 241 NKLYEIGNEDTTALQSGFELVNNYGQSSETDSRKIKCSHCSFCGHPGSKRAHVKFPCEYC 300

Query: 301 DVSHGDGCIKKPDGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAMEPNFPSSELVQIY 360
           DVSHG+GCIKKPDGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAMEPNFPSSEL+Q+Y
Sbjct: 301 DVSHGEGCIKKPDGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAMEPNFPSSELIQMY 360

Query: 361 LGDNHSYFSDDDLILSWGSPRTEMLIDFLVFHLRWEPSYIRQRMLPMLSTIFLREKANNP 420
             DNHSYFSDDDL+LSWGSPRTE+L+DFLVFHL+WEPSYIRQRMLPMLSTIFLRE ANNP
Sbjct: 361 QCDNHSYFSDDDLVLSWGSPRTELLVDFLVFHLQWEPSYIRQRMLPMLSTIFLREMANNP 420

Query: 421 IQTLLYGQYEFDSILREKIRYGHPFYVVKWKKATPTLGNVVNEGSSGGFGTGPEDAIDVD 480
           IQTLL+GQYEFDSILREKIR+GH FYVVKWKKA P + NV+ E S G FGTGPEDAIDVD
Sbjct: 421 IQTLLFGQYEFDSILREKIRFGHSFYVVKWKKAVPAISNVMYEDSLGEFGTGPEDAIDVD 480

Query: 481 EPVDLTDESDSPEIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLQEMEVKRQKTPATPPC 540
           E VDLTDE DSP+IHIQDGCSFLLTDENM+LV AAYPEQAAKFLQE E+KRQKTP+TP C
Sbjct: 481 EAVDLTDEFDSPKIHIQDGCSFLLTDENMELVGAAYPEQAAKFLQEKEMKRQKTPSTPAC 540

Query: 541 GGTSGKSDSSKAKGVQLSITEFYRATKTQQAT-DAGDSTNEGEETSTGKRK-SSSSTLSK 600
            GTSGKSDSSKAKGVQLSITEFYRATKTQQ T D  DSTNE EETSTGKR+ SSSSTLSK
Sbjct: 541 -GTSGKSDSSKAKGVQLSITEFYRATKTQQQTADTTDSTNEDEETSTGKRRASSSSTLSK 600

BLAST of Spg023687 vs. ExPASy TrEMBL
Match: A0A5A7TAP1 (Flap endonuclease GEN-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold122G003140 PE=4 SV=1)

HSP 1 Score: 1073.9 bits (2776), Expect = 2.2e-310
Identity = 541/637 (84.93%), Postives = 570/637 (89.48%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPHARTEGFDFLRNKRIAVDLSFWIVQHETAIKASARSPHLRLTFFRTI 60
           MGVGGHFWDLLKP+ARTEGFDFLRNKR+AVDLSFWIVQHETAIK+++RSPHLRLTFFRTI
Sbjct: 1   MGVGGHFWDLLKPNARTEGFDFLRNKRVAVDLSFWIVQHETAIKSTSRSPHLRLTFFRTI 60

Query: 61  TLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPKIEDRISVDRNRKFAKCV 120
           TLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPK+EDRISV RNRKFAKCV
Sbjct: 61  TLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPKVEDRISVHRNRKFAKCV 120

Query: 121 KECVELLELFGVPVIEAKGEAEALCAELNRKGFVDACITADSDAFLFGAKCVIKSFHPNS 180
           KECVELLELFGVPV+EAKGEAEALCAELN+KGFVDACITADSDAFLFGAKCVIKS HPNS
Sbjct: 121 KECVELLELFGVPVLEAKGEAEALCAELNQKGFVDACITADSDAFLFGAKCVIKSIHPNS 180

Query: 181 KA------------------------------------NIEAALGLNRKHLIAISLLVGN 240
           KA                                    +IEAALGLNR HLIAISLLVGN
Sbjct: 181 KACTPIYTSPLLQVTSLILPCSLEYHHMQEPLECYFMSDIEAALGLNRNHLIAISLLVGN 240

Query: 241 DHDLKGVQGVGLDTAVRFVQDYTDDEILNKLHEIGNEDNTLLQGGLELVNNYGQSSDTNS 300
           DHDL GVQGVGLDTAVRFVQDYTDD+ILNKL+EIGNED T LQ G ELVNNYGQSS+T+S
Sbjct: 241 DHDLNGVQGVGLDTAVRFVQDYTDDQILNKLYEIGNEDTTALQSGFELVNNYGQSSETDS 300

Query: 301 RKIKCSHCSFCGHPGSKRAHVKFPCEYCDVSHGDGCIKKPDGFKCNCSSCDTDREEKEKK 360
           RKIKCSHCSFCGHPGSKRAHVKFPCEYCDVSHG+GCIKKPDGFKCNCSSCDTDREEKEKK
Sbjct: 301 RKIKCSHCSFCGHPGSKRAHVKFPCEYCDVSHGEGCIKKPDGFKCNCSSCDTDREEKEKK 360

Query: 361 KQENWRLRVCNKIAMEPNFPSSELVQIYLGDNHSYFSDDDLILSWGSPRTEMLIDFLVFH 420
           KQENWRLRVCNKIAMEPNFPSSEL+Q+Y  DNHSYFSDDDL+LSWGSPRTE+L+DFLVFH
Sbjct: 361 KQENWRLRVCNKIAMEPNFPSSELIQMYQCDNHSYFSDDDLVLSWGSPRTELLVDFLVFH 420

Query: 421 LRWEPSYIRQRMLPMLSTIFLREKANNPIQTLLYGQYEFDSILREKIRYGHPFYVVKWKK 480
           L+WEPSYIRQRMLPMLSTIFLRE ANNPIQTLL+GQYEFDSILREKIR+GH FYVVKWKK
Sbjct: 421 LQWEPSYIRQRMLPMLSTIFLREMANNPIQTLLFGQYEFDSILREKIRFGHSFYVVKWKK 480

Query: 481 ATPTLGNVVNEGSSGGFGTGPEDAIDVDEPVDLTDESDSPEIHIQDGCSFLLTDENMDLV 540
           A P + NV+ E S G FGTGPEDAIDVDE VDLTDE DSP+IHIQDGCSFLLTDENM+LV
Sbjct: 481 AVPAISNVMYEDSLGEFGTGPEDAIDVDEAVDLTDEFDSPKIHIQDGCSFLLTDENMELV 540

Query: 541 RAAYPEQAAKFLQEMEVKRQKTPATPPCGGTSGKSDSSKAKGVQLSITEFYRATKTQQAT 600
            AAYPEQAAKFLQE E+KRQKTP+TP C GTSGKSDSSKAKGVQLSITEFYRATKTQQ T
Sbjct: 541 GAAYPEQAAKFLQEKEMKRQKTPSTPAC-GTSGKSDSSKAKGVQLSITEFYRATKTQQQT 600

BLAST of Spg023687 vs. ExPASy TrEMBL
Match: A0A6J1DSI1 (flap endonuclease GEN-like 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111023108 PE=4 SV=1)

HSP 1 Score: 1068.9 bits (2763), Expect = 7.6e-309
Identity = 526/607 (86.66%), Postives = 560/607 (92.26%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPHARTEGFDFLRNKRIAVDLSFWIVQHETAIKASARSPHLRLTFFRTI 60
           MGVGGHFWDLLKP+ARTEGFDFLRNKR+AVDLSFWIVQHETAIK +ARSPHLRL+FFRT+
Sbjct: 1   MGVGGHFWDLLKPYARTEGFDFLRNKRVAVDLSFWIVQHETAIKTNARSPHLRLSFFRTV 60

Query: 61  TLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPKIEDRISVDRNRKFAKCV 120
           TLFAKFGAFPVFV+DGTPSPLKSKARI RFFRLSG+D SDLPK+EDRISVDRNRKFAKCV
Sbjct: 61  TLFAKFGAFPVFVLDGTPSPLKSKARIMRFFRLSGMDASDLPKVEDRISVDRNRKFAKCV 120

Query: 121 KECVELLELFGVPVIEAKGEAEALCAELNRKGFVDACITADSDAFLFGAKCVIKSFHPNS 180
           KECVELLELFG+PV+EAKGEAEALCAELN KGFVDACITADSDAFLFGAKCVIKSFHPNS
Sbjct: 121 KECVELLELFGMPVLEAKGEAEALCAELNSKGFVDACITADSDAFLFGAKCVIKSFHPNS 180

Query: 181 K--------ANIEAALGLNRKHLIAISLLVGNDHDLKGVQGVGLDTAVRFVQDYTDDEIL 240
           K        ++IEAALGLNRKHLIAISLLVGNDHDL GVQGVGLDTAVRFVQDYTDDEIL
Sbjct: 181 KEPLECYFMSDIEAALGLNRKHLIAISLLVGNDHDLSGVQGVGLDTAVRFVQDYTDDEIL 240

Query: 241 NKLHEIGNEDNTLLQGGLELVNNYGQSSDTNSRKIKCSHCSFCGHPGSKRAHVKFPCEYC 300
           NKLHEIGN+DN +LQGGL+L N+ GQSSD N RKIKCSHCS+CGHPGSKRAHVK PCEYC
Sbjct: 241 NKLHEIGNKDNRVLQGGLQLANDCGQSSDVNLRKIKCSHCSYCGHPGSKRAHVKSPCEYC 300

Query: 301 DVSHGDGCIKKPDGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAMEPNFPSSELVQIY 360
           D S GDGC+KKP+GFKCNCSSCDTDREEKEKKKQENWRLRVCNKIA+EP FPSSEL+++Y
Sbjct: 301 DASQGDGCVKKPEGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAVEPKFPSSELIKMY 360

Query: 361 LGDNHSYFSDDDLILSWGSPRTEMLIDFLVFHLRWEPSYIRQRMLPMLSTIFLREKANNP 420
           L DNHS+FSDDDLILSWGSPRTEML+DFLVFHLRWEPSYIRQRMLPMLSTIFLRE ANNP
Sbjct: 361 LCDNHSHFSDDDLILSWGSPRTEMLVDFLVFHLRWEPSYIRQRMLPMLSTIFLREMANNP 420

Query: 421 IQTLLYGQYEFDSILREKIRYGHPFYVVKWKKATPTLGNVVNEGSSGGFGTGPEDAIDVD 480
           I TLLYGQYEFDSILREKIRYGH FYVVKWKKA P L NV+ EGSS G GTGPED IDVD
Sbjct: 421 IGTLLYGQYEFDSILREKIRYGHQFYVVKWKKAVPALSNVIYEGSSEGSGTGPEDGIDVD 480

Query: 481 EPVDLTDESDSPEIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLQEMEVKRQKTPATPPC 540
           EPVDLTDESDSP+IHIQDGCSFLLTDENMDLVRAAYPEQAAKFL E E+K+QK   + PC
Sbjct: 481 EPVDLTDESDSPKIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLHEKELKKQKKSTSTPC 540

Query: 541 GGTSGKSDSSKAKGVQLSITEFYRATKTQQATDAGDSTNEGEETSTGKRKSSSSTLSKSV 600
            GTS KSDSSK KGVQLSITEFYR+TK QQA +  DSTNEGEETSTGKRK SS+TLSKSV
Sbjct: 541 -GTSEKSDSSKVKGVQLSITEFYRSTKAQQAAERVDSTNEGEETSTGKRKESSTTLSKSV 600

BLAST of Spg023687 vs. ExPASy TrEMBL
Match: A0A6J1DPU8 (flap endonuclease GEN-like 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111023108 PE=4 SV=1)

HSP 1 Score: 1067.4 bits (2759), Expect = 2.2e-308
Identity = 527/614 (85.83%), Postives = 560/614 (91.21%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPHARTEGFDFLRNKRIAVDLSFWIVQHETAIKASARSPHLRLTFFRTI 60
           MGVGGHFWDLLKP+ARTEGFDFLRNKR+AVDLSFWIVQHETAIK +ARSPHLRL+FFRT+
Sbjct: 1   MGVGGHFWDLLKPYARTEGFDFLRNKRVAVDLSFWIVQHETAIKTNARSPHLRLSFFRTV 60

Query: 61  TLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPKIEDRISVDRNRKFAKCV 120
           TLFAKFGAFPVFV+DGTPSPLKSKARI RFFRLSG+D SDLPK+EDRISVDRNRKFAKCV
Sbjct: 61  TLFAKFGAFPVFVLDGTPSPLKSKARIMRFFRLSGMDASDLPKVEDRISVDRNRKFAKCV 120

Query: 121 KECVELLELFGVPVIEAKGEAEALCAELNRKGFVDACITADSDAFLFGAKCVIKSFHPNS 180
           KECVELLELFG+PV+EAKGEAEALCAELN KGFVDACITADSDAFLFGAKCVIKSFHPNS
Sbjct: 121 KECVELLELFGMPVLEAKGEAEALCAELNSKGFVDACITADSDAFLFGAKCVIKSFHPNS 180

Query: 181 KA---------------NIEAALGLNRKHLIAISLLVGNDHDLKGVQGVGLDTAVRFVQD 240
           KA               +IEAALGLNRKHLIAISLLVGNDHDL GVQGVGLDTAVRFVQD
Sbjct: 181 KAKHLHMQEPLECYFMSDIEAALGLNRKHLIAISLLVGNDHDLSGVQGVGLDTAVRFVQD 240

Query: 241 YTDDEILNKLHEIGNEDNTLLQGGLELVNNYGQSSDTNSRKIKCSHCSFCGHPGSKRAHV 300
           YTDDEILNKLHEIGN+DN +LQGGL+L N+ GQSSD N RKIKCSHCS+CGHPGSKRAHV
Sbjct: 241 YTDDEILNKLHEIGNKDNRVLQGGLQLANDCGQSSDVNLRKIKCSHCSYCGHPGSKRAHV 300

Query: 301 KFPCEYCDVSHGDGCIKKPDGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAMEPNFPS 360
           K PCEYCD S GDGC+KKP+GFKCNCSSCDTDREEKEKKKQENWRLRVCNKIA+EP FPS
Sbjct: 301 KSPCEYCDASQGDGCVKKPEGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAVEPKFPS 360

Query: 361 SELVQIYLGDNHSYFSDDDLILSWGSPRTEMLIDFLVFHLRWEPSYIRQRMLPMLSTIFL 420
           SEL+++YL DNHS+FSDDDLILSWGSPRTEML+DFLVFHLRWEPSYIRQRMLPMLSTIFL
Sbjct: 361 SELIKMYLCDNHSHFSDDDLILSWGSPRTEMLVDFLVFHLRWEPSYIRQRMLPMLSTIFL 420

Query: 421 REKANNPIQTLLYGQYEFDSILREKIRYGHPFYVVKWKKATPTLGNVVNEGSSGGFGTGP 480
           RE ANNPI TLLYGQYEFDSILREKIRYGH FYVVKWKKA P L NV+ EGSS G GTGP
Sbjct: 421 REMANNPIGTLLYGQYEFDSILREKIRYGHQFYVVKWKKAVPALSNVIYEGSSEGSGTGP 480

Query: 481 EDAIDVDEPVDLTDESDSPEIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLQEMEVKRQK 540
           ED IDVDEPVDLTDESDSP+IHIQDGCSFLLTDENMDLVRAAYPEQAAKFL E E+K+QK
Sbjct: 481 EDGIDVDEPVDLTDESDSPKIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLHEKELKKQK 540

Query: 541 TPATPPCGGTSGKSDSSKAKGVQLSITEFYRATKTQQATDAGDSTNEGEETSTGKRKSSS 600
              + PC GTS KSDSSK KGVQLSITEFYR+TK QQA +  DSTNEGEETSTGKRK SS
Sbjct: 541 KSTSTPC-GTSEKSDSSKVKGVQLSITEFYRSTKAQQAAERVDSTNEGEETSTGKRKESS 600

BLAST of Spg023687 vs. TAIR 10
Match: AT1G01880.1 (5'-3' exonuclease family protein )

HSP 1 Score: 597.8 bits (1540), Expect = 9.5e-171
Identity = 317/614 (51.63%), Postives = 423/614 (68.89%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPHARTEGFDFLRNKRIAVDLSFWIVQHETAIKASARSPHLRLTFFRTI 60
           MGVGG+FWDLL+P+A+ +GFDFLRNKR+AVDLSFWIVQHETA+K     PHLRLTFFRTI
Sbjct: 1   MGVGGNFWDLLRPYAQQQGFDFLRNKRVAVDLSFWIVQHETAVKGFVLKPHLRLTFFRTI 60

Query: 61  TLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPKIEDRISVDRNRKFAKCV 120
            LF+KFGA+PVFVVDGTPSPLKS+ARI+RFFR SGIDT +LP I+D +SV+RN+ F++ V
Sbjct: 61  NLFSKFGAYPVFVVDGTPSPLKSQARISRFFRSSGIDTCNLPVIKDGVSVERNKLFSEWV 120

Query: 121 KECVELLELFGVPVIEAKGEAEALCAELNRKGFVDACITADSDAFLFGAKCVIKSFHPNS 180
           +ECVELLEL G+PV++A GEAEALCA+LN +GFVDACIT DSDAFLFGA CVIK   PNS
Sbjct: 121 RECVELLELLGIPVLKANGEAEALCAQLNSQGFVDACITPDSDAFLFGAMCVIKDIKPNS 180

Query: 181 K--------ANIEAALGLNRKHLIAISLLVGNDHDLKGVQGVGLDTAVRFVQDYTDDEIL 240
           +        ++IE+ LGL RKHLIAISLLVGND+D  GV G+G+D A+R V+++++D++L
Sbjct: 181 REPFECYHMSHIESGLGLKRKHLIAISLLVGNDYDSGGVLGIGVDKALRIVREFSEDQVL 240

Query: 241 NKLHEIGNEDNTLLQGGLELVNNYGQSSDTNSRKIKCSHCSFCGHPGSKRAHVKFPCEYC 300
            +L +IGN     + GG++  ++ G+   +  +K +  HCS CGH GSKR H K  CE+C
Sbjct: 241 ERLQDIGNGLQPAVPGGIKSGDD-GEEFRSEMKK-RSPHCSRCGHLGSKRTHFKSSCEHC 300

Query: 301 DVSHGDGCIKKPDGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAMEPNFPSSELVQIY 360
                 GCIKKP GF+C CS C  DR+ +E+KK  +W ++VC+KIA+ P FP+ +++++Y
Sbjct: 301 GCD--SGCIKKPLGFRCECSFCSKDRDLREQKKTNDWWIKVCDKIALAPEFPNRKIIELY 360

Query: 361 LGDNHSYFSDDDLILSWGSPRTEMLIDFLVFHLRWEPSYIRQRMLPMLSTIFLREKA-NN 420
           L D     + D   +SWG+P T ML+D +VF L W+PSY+R+ +LPMLSTI+LREKA NN
Sbjct: 361 LSD--GLMTGDGSSMSWGTPDTGMLVDLMVFKLHWDPSYVRKMLLPMLSTIYLREKARNN 420

Query: 421 PIQTLLYGQYEFDSILREKIRYGHPFYVVKWKKATPTLGNVVNEGSSGGFGTGPEDAIDV 480
               LL  QYEF SI   K RYGH  +V++W+K   T G   +          PE++I V
Sbjct: 421 TGYALLCDQYEFHSIKCIKTRYGHQSFVIRWRKPKSTSGYSHSHSE-------PEESIVV 480

Query: 481 ----DEPVDLTDESDSPEIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLQEMEVKRQKTP 540
               +E VD  D  + P++   +G  FLLTDE + LV++A+P++   FL E +++  K  
Sbjct: 481 LEEEEESVDPLDGLNEPQVQNDNGDCFLLTDECIGLVQSAFPDETEHFLHEKKLRESKKK 540

Query: 541 ATPPCGGTSGKSDSSKAKGVQLSITEFYRATK---TQQATDAGDSTNEGEETSTGKRKSS 599
                      +  +   GVQ SIT+FYR+ K     Q+ + G S+    E       +S
Sbjct: 541 NV---SEEETATPRATTMGVQRSITDFYRSAKKAAAGQSIETGGSSKASAEKKRQATSTS 598

BLAST of Spg023687 vs. TAIR 10
Match: AT1G01880.2 (5'-3' exonuclease family protein )

HSP 1 Score: 592.0 bits (1525), Expect = 5.2e-169
Identity = 316/614 (51.47%), Postives = 422/614 (68.73%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPHARTEGFDFLRNKRIAVDLSFWIVQHETAIKASARSPHLRLTFFRTI 60
           MGVGG+FWDLL+P+A+ +GFDFLRNKR+AVDLSFWIVQHETA+K     PHLRLTFFRTI
Sbjct: 1   MGVGGNFWDLLRPYAQQQGFDFLRNKRVAVDLSFWIVQHETAVKGFVLKPHLRLTFFRTI 60

Query: 61  TLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPKIEDRISVDRNRKFAKCV 120
            LF+KFGA+PVFVVDGTPSPLKS+ARI+RFFR SGIDT +LP I+D +SV+RN+ F++ V
Sbjct: 61  NLFSKFGAYPVFVVDGTPSPLKSQARISRFFRSSGIDTCNLPVIKDGVSVERNKLFSEWV 120

Query: 121 KECVELLELFGVPVIEAKGEAEALCAELNRKGFVDACITADSDAFLFGAKCVIKSFHPNS 180
           +EC ELLEL G+PV++A GEAEALCA+LN +GFVDACIT DSDAFLFGA CVIK   PNS
Sbjct: 121 REC-ELLELLGIPVLKANGEAEALCAQLNSQGFVDACITPDSDAFLFGAMCVIKDIKPNS 180

Query: 181 K--------ANIEAALGLNRKHLIAISLLVGNDHDLKGVQGVGLDTAVRFVQDYTDDEIL 240
           +        ++IE+ LGL RKHLIAISLLVGND+D  GV G+G+D A+R V+++++D++L
Sbjct: 181 REPFECYHMSHIESGLGLKRKHLIAISLLVGNDYDSGGVLGIGVDKALRIVREFSEDQVL 240

Query: 241 NKLHEIGNEDNTLLQGGLELVNNYGQSSDTNSRKIKCSHCSFCGHPGSKRAHVKFPCEYC 300
            +L +IGN     + GG++  ++ G+   +  +K +  HCS CGH GSKR H K  CE+C
Sbjct: 241 ERLQDIGNGLQPAVPGGIKSGDD-GEEFRSEMKK-RSPHCSRCGHLGSKRTHFKSSCEHC 300

Query: 301 DVSHGDGCIKKPDGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAMEPNFPSSELVQIY 360
                 GCIKKP GF+C CS C  DR+ +E+KK  +W ++VC+KIA+ P FP+ +++++Y
Sbjct: 301 GCD--SGCIKKPLGFRCECSFCSKDRDLREQKKTNDWWIKVCDKIALAPEFPNRKIIELY 360

Query: 361 LGDNHSYFSDDDLILSWGSPRTEMLIDFLVFHLRWEPSYIRQRMLPMLSTIFLREKA-NN 420
           L D     + D   +SWG+P T ML+D +VF L W+PSY+R+ +LPMLSTI+LREKA NN
Sbjct: 361 LSD--GLMTGDGSSMSWGTPDTGMLVDLMVFKLHWDPSYVRKMLLPMLSTIYLREKARNN 420

Query: 421 PIQTLLYGQYEFDSILREKIRYGHPFYVVKWKKATPTLGNVVNEGSSGGFGTGPEDAIDV 480
               LL  QYEF SI   K RYGH  +V++W+K   T G   +          PE++I V
Sbjct: 421 TGYALLCDQYEFHSIKCIKTRYGHQSFVIRWRKPKSTSGYSHSHSE-------PEESIVV 480

Query: 481 ----DEPVDLTDESDSPEIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLQEMEVKRQKTP 540
               +E VD  D  + P++   +G  FLLTDE + LV++A+P++   FL E +++  K  
Sbjct: 481 LEEEEESVDPLDGLNEPQVQNDNGDCFLLTDECIGLVQSAFPDETEHFLHEKKLRESKKK 540

Query: 541 ATPPCGGTSGKSDSSKAKGVQLSITEFYRATK---TQQATDAGDSTNEGEETSTGKRKSS 599
                      +  +   GVQ SIT+FYR+ K     Q+ + G S+    E       +S
Sbjct: 541 NV---SEEETATPRATTMGVQRSITDFYRSAKKAAAGQSIETGGSSKASAEKKRQATSTS 597

BLAST of Spg023687 vs. TAIR 10
Match: AT3G48900.2 (single-stranded DNA endonuclease family protein )

HSP 1 Score: 115.2 bits (287), Expect = 1.9e-25
Identity = 84/253 (33.20%), Postives = 125/253 (49.41%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPHARTEGFDFLRNKRIAVDLSFWIVQHETAIK---ASARSPHLRLTFF 60
           MGV  + WD+L+P  +T   D L+NKR+ VDLS W+V+     K   A+    +LR  F 
Sbjct: 1   MGV-KYLWDVLEPCKKTFPLDHLQNKRVCVDLSCWMVELHKVNKSYCATKEKVYLRGFFH 60

Query: 61  RTITLFAKFGAFPVFVVDGTPSPLK---SKARIARFFRLSGIDTSDLPKIEDRISVDRNR 120
           R   L A      + V DG    +K    K R+   F ++  D    P  E  +  +   
Sbjct: 61  RLRALIA-LNCSIILVSDGAIPGIKVPTYKRRLKARFEIA--DDGVEPSKETSLKRNMGS 120

Query: 121 KFAKCVKECVELLELFGVPVIEAKGEAEALCAELNRKGFVDACITADSDAFLFGAKCVIK 180
           +F+  +KE   +    G+  ++   EAEA CA LN +   DAC + DSD FLFGAK V +
Sbjct: 121 EFSCIIKEAKVIASTLGILCLDGIEEAEAQCALLNSESLCDACFSFDSDIFLFGAKTVYR 180

Query: 181 SFHPNSKA--------NIEAALGLNRKHLIAISLLVGNDHDLKGVQGVGLDTAVRFVQDY 240
                           +I+  LGL R  LIA++LL+G+D+  +GV+G+  + A   V+  
Sbjct: 181 EICLGEGGYVVCYEMDDIKKKLGLGRNSLIALALLLGSDYS-QGVRGLRQEKACELVRSI 240

BLAST of Spg023687 vs. TAIR 10
Match: AT3G28030.1 (5'-3' exonuclease family protein )

HSP 1 Score: 94.7 bits (234), Expect = 2.6e-19
Identity = 78/299 (26.09%), Postives = 128/299 (42.81%), Query Frame = 0

Query: 122  ECVELLELFGVPVIEAKGEAEALCAELNRKGFVDACITADSDAFLFGAKCVIKSFHPNSK 181
            EC ELL++FG+P I A  EAEA CA + +   VD  +T DSD FLFGA+ V K+   + K
Sbjct: 928  ECQELLQIFGIPYIIAPMEAEAQCAFMEQSNLVDGIVTDDSDVFLFGARSVYKNIFDDRK 987

Query: 182  -------ANIEAALGLNRKHLIAISLLVGNDHDLKGVQGVGLDTAVRFVQDYTDDEILNK 241
                    +IE  LGL+R  +I +++L+G+D+  +G+ G+G+  A+  V  + +++ L K
Sbjct: 988  YVETYFMKDIEKELGLSRDKIIRMAMLLGSDY-TEGISGIGIVNAIEVVTAFPEEDGLQK 1047

Query: 242  LHE-IGNEDNTLLQGGLELVNNYGQSSDTNSRKIKCSHCSFCGHPGSKRAHVKFPCEYCD 301
              E + + D T+L          G++      K+K                         
Sbjct: 1048 FREWVESPDPTIL----------GKTDAKTGSKVK------------------------- 1107

Query: 302  VSHGDGCIKKPDGFKCNCSSCDTDR-----EEKEKKKQENWRLRVCNKIAMEPNFPSSEL 361
               G   +    G     S+ DT+       ++ +K  +NW + +         FPS  +
Sbjct: 1108 -KRGSASVDN-KGIISGASTDDTEEIKQIFMDQHRKVSKNWHIPL--------TFPSEAV 1167

Query: 362  VQIYLGDNHSYFSDDDLILSWGSPRTEMLIDFLVFHLRWEPSYIRQRMLPMLSTIFLRE 408
            +  YL       ++     SWG P   +L         W      + +LP+L     RE
Sbjct: 1168 ISAYLNPQVDLSTEK---FSWGKPDLSVLRKLCWEKFNWNGKKTDELLLPVLKEYEKRE 1177

BLAST of Spg023687 vs. TAIR 10
Match: AT5G26680.1 (5'-3' exonuclease family protein )

HSP 1 Score: 82.4 bits (202), Expect = 1.3e-15
Identity = 74/250 (29.60%), Postives = 112/250 (44.80%), Query Frame = 0

Query: 16  RTEGFDFLRNKRIAVDLSFWIVQHETAI----------KASARSPHLRLTFFRTITLFAK 75
           + + F+    ++IAVD S  I Q    +          +A   + HL+  F RTI L  +
Sbjct: 19  KEQKFESYFGRKIAVDASMSIYQFLIVVGRTGTEMLTNEAGEVTSHLQGMFNRTIRLL-E 78

Query: 76  FGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPKIEDRISVDRNRKFAKCVK---- 135
            G  PV+V DG P  LK +    R+ + +         IE     D  +   + VK    
Sbjct: 79  AGIKPVYVFDGKPPELKRQELAKRYSKRADATADLTGAIEAGNKEDIEKYSKRTVKVTKQ 138

Query: 136 ---ECVELLELFGVPVIEAKGEAEALCAELNRKGFVDACITADSDAFLFGA-KCVIKSFH 195
              +C  LL L GVPV+EA  EAEA CA L + G V    + D D+  FGA K +     
Sbjct: 139 HNDDCKRLLRLMGVPVVEATSEAEAQCAALCKSGKVYGVASEDMDSLTFGAPKFLRHLMD 198

Query: 196 PNSK---------ANIEAALGLNRKHLIAISLLVGNDHDLKGVQGVGLDTAVRFVQDYTD 238
           P+S+         A I   L L     I + +L G D+    ++G+G  TA++ ++ +  
Sbjct: 199 PSSRKIPVMEFEVAKILEELQLTMDQFIDLCILSGCDY-CDSIRGIGGQTALKLIRQHGS 258

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038876412.10.0e+0089.66flap endonuclease GEN-like 1 isoform X2 [Benincasa hispida][more]
XP_038876411.10.0e+0086.01flap endonuclease GEN-like 1 isoform X1 [Benincasa hispida][more]
TYK24473.10.0e+0088.67flap endonuclease GEN-like 1 [Cucumis melo var. makuwa][more]
XP_008460162.10.0e+0088.51PREDICTED: flap endonuclease GEN-like 1 [Cucumis melo][more]
KAA0040028.14.6e-31084.93flap endonuclease GEN-like 1 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q9LPD21.3e-16951.63Flap endonuclease GEN-like 1 OS=Arabidopsis thaliana OX=3702 GN=GEN1 PE=1 SV=3[more]
Q64MA33.4e-15748.20Flap endonuclease GEN-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN=RAD PE=... [more]
Q8BMI41.3e-4427.55Flap endonuclease GEN homolog 1 OS=Mus musculus OX=10090 GN=Gen1 PE=1 SV=2[more]
Q17RS78.2e-4227.32Flap endonuclease GEN homolog 1 OS=Homo sapiens OX=9606 GN=GEN1 PE=1 SV=2[more]
Q9VRJ01.9e-3523.86Flap endonuclease GEN OS=Drosophila melanogaster OX=7227 GN=Gen PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3DLF90.0e+0088.67Flap endonuclease GEN-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3CD600.0e+0088.51flap endonuclease GEN-like 1 OS=Cucumis melo OX=3656 GN=LOC103499055 PE=4 SV=1[more]
A0A5A7TAP12.2e-31084.93Flap endonuclease GEN-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
A0A6J1DSI17.6e-30986.66flap endonuclease GEN-like 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A6J1DPU82.2e-30885.83flap endonuclease GEN-like 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111... [more]
Match NameE-valueIdentityDescription
AT1G01880.19.5e-17151.635'-3' exonuclease family protein [more]
AT1G01880.25.2e-16951.475'-3' exonuclease family protein [more]
AT3G48900.21.9e-2533.20single-stranded DNA endonuclease family protein [more]
AT3G28030.12.6e-1926.095'-3' exonuclease family protein [more]
AT5G26680.11.3e-1529.605'-3' exonuclease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006084XPG/Rad2 endonucleasePRINTSPR00853XPGRADSUPERcoord: 25..39
score: 35.62
coord: 70..89
score: 39.38
coord: 148..168
score: 45.24
coord: 127..144
score: 48.44
IPR006084XPG/Rad2 endonucleasePANTHERPTHR11081FLAP ENDONUCLEASE FAMILY MEMBERcoord: 1..587
IPR006085XPG, N-terminalSMARTSM00485xpgn3coord: 1..96
e-value: 2.3E-4
score: 25.8
IPR006085XPG, N-terminalPFAMPF00752XPG_Ncoord: 1..86
e-value: 9.1E-9
score: 35.7
IPR006086XPG-I domainSMARTSM00484xpgineucoord: 128..190
e-value: 8.3E-14
score: 61.9
IPR006086XPG-I domainPFAMPF00867XPG_Icoord: 129..205
e-value: 2.0E-18
score: 66.5
NoneNo IPR availableGENE3D1.10.150.20coord: 193..266
e-value: 7.8E-8
score: 34.1
NoneNo IPR availableGENE3D3.40.50.1010coord: 1..192
e-value: 1.3E-41
score: 144.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 529..573
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 521..600
NoneNo IPR availablePANTHERPTHR11081:SF59FLAP ENDONUCLEASE GEN-LIKE 1coord: 1..587
NoneNo IPR availableCDDcd09869PIN_GEN1coord: 2..194
e-value: 3.42119E-77
score: 242.51
IPR0362795'-3' exonuclease, C-terminal domain superfamilySUPERFAMILY478075' to 3' exonuclease, C-terminal subdomaincoord: 190..406
IPR029060PIN-like domain superfamilySUPERFAMILY88723PIN domain-likecoord: 6..205

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg023687.1Spg023687.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006259 DNA metabolic process
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0009555 pollen development
cellular_component GO:0005634 nucleus
molecular_function GO:0017108 5'-flap endonuclease activity
molecular_function GO:0003690 double-stranded DNA binding
molecular_function GO:0003697 single-stranded DNA binding
molecular_function GO:0004518 nuclease activity