Spg023668 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg023668
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionE3 ubiquitin-protein ligase RHF1A
Locationscaffold13: 2675754 .. 2679033 (-)
RNA-Seq ExpressionSpg023668
SyntenySpg023668
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACCCGGTTTGGTAGGAAGGATTAAATTAGATTGCGTCAGTTTGGATTTAAAAGAAAGAAAGATAGGAAAATGGAAATGGGAAGTTGAGAGCAGACTGATTCCACCAAACCGTATGAATCGGTTTGTTTGTTTGCTGCAATTTTTCTTCAGCCTTTTCCTCTTCTCCCCATAACTCTCTTCTTTGACCATTCTCTCTCTCTCTCTCTGCTCTTTTCTAGGTTTCAGTTTTCCCCTTTTCCTCTTCTAAATTCCTTCCTCAACCCTCTTTGATTTGTCGGCTCGGCTCTCCATGGCGAATTTCACTTTCACTTCCACTTCCACTTCCACTCCTCCTTTGCTTTCTGCTGCTTCCGATGATGCTTTTGAAGACGCCTGCTGCATTTGCCTCGACCCCTTTACGTCCCATGACCCTGCCACTGTAAGTTCATCACTCAACTTCCTTCTTCTTGACTGCACATTGTTACTTTGTTCGATTCTGATGATGGGTTGTGCGTATTTGAGCTCTCTTCTGAAGGAACATTGAGTTTTGTGTATTTTGTACTTGCATCGCTTTTGGAATTTGCTGCTTTTTGGAGATCATGTTCCCTTATCTACCTGGTTTTGCAACTTTTCTGTTTTGGGTCCTTGAGTTTTGGGGAGAATTGTTGATCATACTGACAATGAAAGAAGGAGGAGACTTGTTTTTTCTGGAGAAATGTGTGAGAAAGGGTATCTTTTTGATGCAAATGAGCCAACAGTTCATTAGCTTGCTCTTAATTGAATCATCTTCTACCAAATGTACTTTGTGATCTTCATCTGATCTTGGTTTTTCTATCCCCCTCTCCCTTCTCAAATTGGGTTCTTTGTTTAACTTCCAATTGGGGGTTTACAGGATAATTTTATACCCTTCGTACAATCAATGAAAAGCATCTCTAGCTAAAGAAAGAGCTTCTAGATTTGATTCTTTTAGCTAGGCCATGAGCTTTGGCTCTTACTTTTACTTGTGGAAATTAGTTTCTTAACTAACAATTCATGTCTTCAGAAAAGAGTTTTGTGCTTTCTTGTTTATCAGTATGAACCAAAGTGTCACTGTAGAATGTAGGTGAGTTTATTTCTGATGACGGTTGTTGTAATGTTGTTCATCTTGTGTTCCGGGATTCTTTTGTTTTCTATCATCAAGCTTCTTTGGTTGGAATGCAATGACATGGTATGATTTAGCGAGACTAAGATTGACTGTCCAAGTGAATGGTCATATTTACTGTGTTAGAAAGTTGAGATCCAATTAAGCCATTTTTCTCTCCCTATTGCTGAGGAATTTTTGTCTCAAAGATGTTGCTACTTCATTCTAGGTGTTTTGTGTGGCCTTTACATTTGCATTAACTGTGTCTGTTGTTCCAATTGCAGATTACCAGTTGCAAGCATGAATACCATCTCCAATGTATTCTTGATTGGTAGGCTTGTTGAAGAATTCTTTTCATCTTATCTTCTAAAAACAGGTTGATGTAAGTCTTACTTTTTGGGTATTGATTCTTTCAGGTCTCAAAGAAGCAATGAGTGCCCAATCTGTTGCCAGTTGCTAGTGTTGAAGGATCCTGTTGGGTATGTAACTTTGTCATAGAAAGTTCAATTCCTCTTGCATTTTCCTCCCAATTCTCATCCTCGCCCTATCTCTCTCAATCTATCTAATGAATGAGAATGATGCAAGCTTTCCAATTAATCATGTGCTAATTTTGCTATTCTACAGCCAGGAGCTTCTAGCAGCCATGGCTAGTGAAAGGCTTTTAAAGTCGAGAAGCTTGTCTTCTGCTGCATCCACTTCTCTGCGTTTCCATGAAAACTTTGATATTGACCACGTGAGTAGTTTTAATAAATCTGACACATTATTGGACCACCTTATGCTTAATCGTTGCTTCAATGAGGATCAACCATTTATTAAGCAGCAAAATTGATTCCTTCAGGACTCCTCTCACTCGGATGACTCTGACTTTGATGACCTGATTATGCAGCATCTTGCTGCTGCAGCTAGTAGAGCACGGTATGTTCATAGAAGGGAAAGGCAAAGACATTTTGGGCTCGGGCCTTCGCAAGTGGCCTCTGCATCTCCAGAAGAGGTTCCAGTGCAGATATGCCCATTGTCGCCACGATTTCAAGATGCAACTCTAAATTCGCCAAATAGTGATTCCCCATCTCCCGGTTCCCTTGATGAGAATGAGTTCTCAAGAACTAGAATTAGTAAAATCTCACCTAGGTACATTACATCCCTGGACTAATGTATCTACTGCCCGCTTTGCTCGTTCTGAACACCAATAATAAAGTAAATTAACTTCTACGTTGCTTTATTCCGCTTGGGATGCAAAAAGTTAGCAGTAGAAGAGAGAACGCCTTGCTAGTAATCTATCAAGAAAGTCTTTCTTCCCTGGTTACTACCTTAAATTTTCAAGATGCCCTCAATCCTCAAAAACAAGTAAAGCTCTCATTTGTTTTACTGTCTAACATAGGTCAAACTTCTCTTGTCATGTAGGGTTCTACTGACTGAGACATCCTCGGGTAGTCAACTGAAAACAAACCAATCTGAGGGCCTATCTTTCCCAGATTCCATCAAGTCCAAATGGTCTGCTACTTCTGCAAAGTAAGTGTAATGGAGTTTGCATCTCTTAAATTAGTTTCTACATGTCACATCATCAATGCTAACATGCATATCAAGATTACTAAATCTGTAACAATACAGGTACAAGGAGTCAATCTGGCGAGGCACTCAAGGTATTAAGGAGAAACTGCTTGCTCGTAACAGCTCTGTGAGGGAGCTAAGCAGAGGAGTAAAGCGCGAAGTGAGTGCTGGGATTGCTGGTGTTTCTCGAATGATTGAGCGACTCGATTTGACCACAAAAAAGAATGCTGCATCTGTTTCCTTTTTCAGCTGCAGTGGGGGTACATCAAACTCCTTAAAAGGAAAGAACGTAGTGCAAGAGAGTGCTGTCACAGATGGTTCAGTTAAAATTGATAGAATATGTACTGGTTCACCCTCTCAGGTTTCCCCAACTGTTCCAGCCGCAGTCGAAGTTTCTCTTGCTCAGGTACTTAACTTTATGTACTTTAGACGATTTCCAACGTAAACTATTCTTGTTTTCGAGTTAACTTCTTCATCGACTTTCGCTTATGTGCTTGATATTGTGTACATTATATTGTGCATTGTTCACAATGAGGCACATGTAAACTTATGTGAAAACCTCCACAATCTACATATTTGATATGTTTATTGATTAAACATTCATTTCTTTGGCAGAGAGGGGATTGATGTGTG

mRNA sequence

ATGGCGAATTTCACTTTCACTTCCACTTCCACTTCCACTCCTCCTTTGCTTTCTGCTGCTTCCGATGATGCTTTTGAAGACGCCTGCTGCATTTGCCTCGACCCCTTTACGTCCCATGACCCTGCCACTATTACCAGTTGCAAGCATGAATACCATCTCCAATGTATTCTTGATTGGTCTCAAAGAAGCAATGAGTGCCCAATCTGTTGCCAGTTGCTAGTGTTGAAGGATCCTGTTGGCCAGGAGCTTCTAGCAGCCATGGCTAGTGAAAGGCTTTTAAAGTCGAGAAGCTTGTCTTCTGCTGCATCCACTTCTCTGCGTTTCCATGAAAACTTTGATATTGACCACGACTCCTCTCACTCGGATGACTCTGACTTTGATGACCTGATTATGCAGCATCTTGCTGCTGCAGCTAGTAGAGCACGGTATGTTCATAGAAGGGAAAGGCAAAGACATTTTGGGCTCGGGCCTTCGCAAGTGGCCTCTGCATCTCCAGAAGAGGTTCCAGTGCAGATATGCCCATTGTCGCCACGATTTCAAGATGCAACTCTAAATTCGCCAAATAGTGATTCCCCATCTCCCGGTTCCCTTGATGAGAATGAGTTCTCAAGAACTAGAATTAGTAAAATCTCACCTAGGGTTCTACTGACTGAGACATCCTCGGGTAGTCAACTGAAAACAAACCAATCTGAGGGCCTATCTTTCCCAGATTCCATCAAGTCCAAATGGTCTGCTACTTCTGCAAAGTACAAGGAGTCAATCTGGCGAGGCACTCAAGGTATTAAGGAGAAACTGCTTGCTCGTAACAGCTCTGTGAGGGAGCTAAGCAGAGGAGTAAAGCGCGAAGTGAGTGCTGGGATTGCTGGTGTTTCTCGAATGATTGAGCGACTCGATTTGACCACAAAAAAGAATGCTGCATCTGTTTCCTTTTTCAGCTGCAGTGGGGGTACATCAAACTCCTTAAAAGGAAAGAACGTAGTGCAAGAGAGTGCTGTCACAGATGGTTCAGTTAAAATTGATAGAATATGTACTGGTTCACCCTCTCAGGTTTCCCCAACTGTTCCAGCCGCAGTCGAAGTTTCTCTTGCTCAGGTACTTAACTTTATGTACTTTAGACGATTTCCAACGTAA

Coding sequence (CDS)

ATGGCGAATTTCACTTTCACTTCCACTTCCACTTCCACTCCTCCTTTGCTTTCTGCTGCTTCCGATGATGCTTTTGAAGACGCCTGCTGCATTTGCCTCGACCCCTTTACGTCCCATGACCCTGCCACTATTACCAGTTGCAAGCATGAATACCATCTCCAATGTATTCTTGATTGGTCTCAAAGAAGCAATGAGTGCCCAATCTGTTGCCAGTTGCTAGTGTTGAAGGATCCTGTTGGCCAGGAGCTTCTAGCAGCCATGGCTAGTGAAAGGCTTTTAAAGTCGAGAAGCTTGTCTTCTGCTGCATCCACTTCTCTGCGTTTCCATGAAAACTTTGATATTGACCACGACTCCTCTCACTCGGATGACTCTGACTTTGATGACCTGATTATGCAGCATCTTGCTGCTGCAGCTAGTAGAGCACGGTATGTTCATAGAAGGGAAAGGCAAAGACATTTTGGGCTCGGGCCTTCGCAAGTGGCCTCTGCATCTCCAGAAGAGGTTCCAGTGCAGATATGCCCATTGTCGCCACGATTTCAAGATGCAACTCTAAATTCGCCAAATAGTGATTCCCCATCTCCCGGTTCCCTTGATGAGAATGAGTTCTCAAGAACTAGAATTAGTAAAATCTCACCTAGGGTTCTACTGACTGAGACATCCTCGGGTAGTCAACTGAAAACAAACCAATCTGAGGGCCTATCTTTCCCAGATTCCATCAAGTCCAAATGGTCTGCTACTTCTGCAAAGTACAAGGAGTCAATCTGGCGAGGCACTCAAGGTATTAAGGAGAAACTGCTTGCTCGTAACAGCTCTGTGAGGGAGCTAAGCAGAGGAGTAAAGCGCGAAGTGAGTGCTGGGATTGCTGGTGTTTCTCGAATGATTGAGCGACTCGATTTGACCACAAAAAAGAATGCTGCATCTGTTTCCTTTTTCAGCTGCAGTGGGGGTACATCAAACTCCTTAAAAGGAAAGAACGTAGTGCAAGAGAGTGCTGTCACAGATGGTTCAGTTAAAATTGATAGAATATGTACTGGTTCACCCTCTCAGGTTTCCCCAACTGTTCCAGCCGCAGTCGAAGTTTCTCTTGCTCAGGTACTTAACTTTATGTACTTTAGACGATTTCCAACGTAA

Protein sequence

MANFTFTSTSTSTPPLLSAASDDAFEDACCICLDPFTSHDPATITSCKHEYHLQCILDWSQRSNECPICCQLLVLKDPVGQELLAAMASERLLKSRSLSSAASTSLRFHENFDIDHDSSHSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQVASASPEEVPVQICPLSPRFQDATLNSPNSDSPSPGSLDENEFSRTRISKISPRVLLTETSSGSQLKTNQSEGLSFPDSIKSKWSATSAKYKESIWRGTQGIKEKLLARNSSVRELSRGVKREVSAGIAGVSRMIERLDLTTKKNAASVSFFSCSGGTSNSLKGKNVVQESAVTDGSVKIDRICTGSPSQVSPTVPAAVEVSLAQVLNFMYFRRFPT
Homology
[Close]
BLAST of Spg023668 vs. NCBI nr
Match: XP_023549293.1 (E3 ubiquitin-protein ligase RHF1A isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 584.3 bits (1505), Expect = 7.3e-163
Identity = 313/366 (85.52%), Postives = 334/366 (91.26%), Query Frame = 0 

Query: 1   MANFTFTSTSTSTPPLLSAASDDAFE-DACCICLDPFTSHDPATITSCKHEYHLQCILDW 60
           MA+FT +S +      LS+ SDDAFE DACCICLDPFTSHDP +ITSCKHEYHLQCILDW
Sbjct: 1   MADFTSSSLNA-----LSSPSDDAFEDDACCICLDPFTSHDPPSITSCKHEYHLQCILDW 60

Query: 61  SQRSNECPICCQLLVLKDPVGQELLAAMASERLLKSRSLSSAASTSLRFHENFDIDHDSS 120
           SQRSNECPICCQLLVL+DPVGQELLAA+ASE+LLKSRSLSS ASTSLRFHENFD+DHDSS
Sbjct: 61  SQRSNECPICCQLLVLRDPVGQELLAAIASEKLLKSRSLSSVASTSLRFHENFDVDHDSS 120

Query: 121 HSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQVASASPEEVPVQICPLSPRF 180
           HSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQ ASA PEEVPV ICPLSPRF
Sbjct: 121 HSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQGASACPEEVPVLICPLSPRF 180

Query: 181 QDATLNSPNSDSPSPGSLDENEFSRTRISKISPRVLLTETSSGSQLKTNQSEGLSFPDSI 240
           QDATL+SPNSDSPSPGS   +   RT ISK++PRVLLTETSSGSQ KTNQSEGLSFPDSI
Sbjct: 181 QDATLSSPNSDSPSPGS-PLSSGGRTGISKVTPRVLLTETSSGSQQKTNQSEGLSFPDSI 240

Query: 241 KSKWSATSAKYKESIWRGTQGIKEKLLARNSSVRELSRGVKREVSAGIAGVSRMIERLDL 300
           KSKWSATSAKYKESIWR TQGIKEKLLARNSSV+ELSRGVKREVSAGIAGV+RMI+RLDL
Sbjct: 241 KSKWSATSAKYKESIWRSTQGIKEKLLARNSSVKELSRGVKREVSAGIAGVARMIDRLDL 300

Query: 301 TTKKNAASVSFFSCSGGTSNSLKGKNVV-QESAVTDGSVKIDRICTGSPSQVSPTVPAAV 360
           ++K+NAASVSFFSCSGGTSNS+KGKNVV QES VTDGS K DRICTGSPS VSPTVP  V
Sbjct: 301 SSKRNAASVSFFSCSGGTSNSMKGKNVVMQESGVTDGSEKNDRICTGSPSHVSPTVPGTV 360

Query: 361 EVSLAQ 365
           + SLAQ
Sbjct: 361 DDSLAQ 360

[Close]
BLAST of Spg023668 vs. NCBI nr
Match: XP_023006440.1 (E3 ubiquitin-protein ligase RHF1A isoform X1 [Cucurbita maxima])

HSP 1 Score: 583.9 bits (1504), Expect = 9.6e-163
Identity = 313/366 (85.52%), Postives = 332/366 (90.71%), Query Frame = 0 

Query: 1   MANFTFTSTSTSTPPLLSAASDDAFE-DACCICLDPFTSHDPATITSCKHEYHLQCILDW 60
           MA+FT +S +  +PP     SDDAFE DACCICLDPFTSHDP +ITSCKHEYHLQCILDW
Sbjct: 1   MADFTSSSLNALSPP-----SDDAFEDDACCICLDPFTSHDPPSITSCKHEYHLQCILDW 60

Query: 61  SQRSNECPICCQLLVLKDPVGQELLAAMASERLLKSRSLSSAASTSLRFHENFDIDHDSS 120
           SQRSNECPICCQLLVL+DPVGQELLAA+ASE+LLKSRSLSS ASTSLRFHENFD+DHDSS
Sbjct: 61  SQRSNECPICCQLLVLRDPVGQELLAAIASEKLLKSRSLSSVASTSLRFHENFDVDHDSS 120

Query: 121 HSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQVASASPEEVPVQICPLSPRF 180
           HSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQ  SA PEEVPV ICPLSPRF
Sbjct: 121 HSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQGVSACPEEVPVLICPLSPRF 180

Query: 181 QDATLNSPNSDSPSPGSLDENEFSRTRISKISPRVLLTETSSGSQLKTNQSEGLSFPDSI 240
           QDATL+SPNSDSPSPGS   +   RT ISKISPRVLLTET SGSQ KTNQ EGLSFPDSI
Sbjct: 181 QDATLSSPNSDSPSPGS-PLSSGGRTGISKISPRVLLTETLSGSQQKTNQPEGLSFPDSI 240

Query: 241 KSKWSATSAKYKESIWRGTQGIKEKLLARNSSVRELSRGVKREVSAGIAGVSRMIERLDL 300
           KSKWSATSAKYKESIWR TQGIKEKLLARNSSV+ELSRGVKREVSAGIAGV+RMI+RLDL
Sbjct: 241 KSKWSATSAKYKESIWRSTQGIKEKLLARNSSVKELSRGVKREVSAGIAGVARMIDRLDL 300

Query: 301 TTKKNAASVSFFSCSGGTSNSLKGKNVV-QESAVTDGSVKIDRICTGSPSQVSPTVPAAV 360
           ++K+NAASVSFFSCSGGTSNS+KGKNVV QES VTDGSVK DRICTGSPS VSPTVP +V
Sbjct: 301 SSKRNAASVSFFSCSGGTSNSMKGKNVVMQESGVTDGSVKNDRICTGSPSHVSPTVPGSV 360

Query: 361 EVSLAQ 365
             SLAQ
Sbjct: 361 NDSLAQ 360

[Close]
BLAST of Spg023668 vs. NCBI nr
Match: XP_022959383.1 (E3 ubiquitin-protein ligase RHF1A isoform X1 [Cucurbita moschata])

HSP 1 Score: 581.6 bits (1498), Expect = 4.7e-162
Identity = 312/366 (85.25%), Postives = 334/366 (91.26%), Query Frame = 0 

Query: 1   MANFTFTSTSTSTPPLLSAASDDAFE-DACCICLDPFTSHDPATITSCKHEYHLQCILDW 60
           MA+FT +S +  +PP     SDDAFE DACCICLDPFTSHDP +ITSCKHEYHLQCILDW
Sbjct: 1   MADFTSSSLNALSPP-----SDDAFEDDACCICLDPFTSHDPPSITSCKHEYHLQCILDW 60

Query: 61  SQRSNECPICCQLLVLKDPVGQELLAAMASERLLKSRSLSSAASTSLRFHENFDIDHDSS 120
           SQRSNECPICCQLLVL+DPVGQELLAA+A+E+LLKSRSLSS ASTSLRFHENFD+DHDSS
Sbjct: 61  SQRSNECPICCQLLVLRDPVGQELLAAIANEKLLKSRSLSSVASTSLRFHENFDVDHDSS 120

Query: 121 HSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQVASASPEEVPVQICPLSPRF 180
            SDDSDFDDLIMQHLAAAASRARYVHRRERQR FGLGPSQ ASA PEEVPV ICPLSPRF
Sbjct: 121 RSDDSDFDDLIMQHLAAAASRARYVHRRERQRQFGLGPSQGASACPEEVPVLICPLSPRF 180

Query: 181 QDATLNSPNSDSPSPGSLDENEFSRTRISKISPRVLLTETSSGSQLKTNQSEGLSFPDSI 240
           QDA+L+SPNSDSPSPGS   +   RT ISKISPRVLLTETSSGSQ KTNQSEGLSFPDSI
Sbjct: 181 QDASLSSPNSDSPSPGS-PLSSGGRTGISKISPRVLLTETSSGSQQKTNQSEGLSFPDSI 240

Query: 241 KSKWSATSAKYKESIWRGTQGIKEKLLARNSSVRELSRGVKREVSAGIAGVSRMIERLDL 300
           KSKWSATSAKYKESIWR TQGIKEKLLARNSSV+ELSRGVKREVSAGIAGV+RMI+RLDL
Sbjct: 241 KSKWSATSAKYKESIWRSTQGIKEKLLARNSSVKELSRGVKREVSAGIAGVARMIDRLDL 300

Query: 301 TTKKNAASVSFFSCSGGTSNSLKGKNVV-QESAVTDGSVKIDRICTGSPSQVSPTVPAAV 360
           ++K+NAASVSFFSCSGGTSNS+KGKNVV QES VTDGSVK DRICTGSPS VSPTVP +V
Sbjct: 301 SSKRNAASVSFFSCSGGTSNSMKGKNVVMQESGVTDGSVKNDRICTGSPSHVSPTVPGSV 360

Query: 361 EVSLAQ 365
           + SLAQ
Sbjct: 361 DDSLAQ 360

[Close]
BLAST of Spg023668 vs. NCBI nr
Match: KAG6575008.1 (E3 ubiquitin-protein ligase RHF1A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 579.7 bits (1493), Expect = 1.8e-161
Identity = 311/366 (84.97%), Postives = 333/366 (90.98%), Query Frame = 0 

Query: 1   MANFTFTSTSTSTPPLLSAASDDAFE-DACCICLDPFTSHDPATITSCKHEYHLQCILDW 60
           MA+FT +S +  +PP     SDDAFE DACCICLDPFTSHDP +IT CKHEYHLQCILDW
Sbjct: 1   MADFTSSSLNALSPP-----SDDAFEDDACCICLDPFTSHDPPSITRCKHEYHLQCILDW 60

Query: 61  SQRSNECPICCQLLVLKDPVGQELLAAMASERLLKSRSLSSAASTSLRFHENFDIDHDSS 120
           SQRSNECPICCQLLVL+DPVGQELLAA+A+E+LLKSRSLSS ASTSLRFHENFD+DHDSS
Sbjct: 61  SQRSNECPICCQLLVLRDPVGQELLAAIANEKLLKSRSLSSVASTSLRFHENFDVDHDSS 120

Query: 121 HSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQVASASPEEVPVQICPLSPRF 180
            SDDSDFDDLIMQHLAAAASRARYVHRRERQR FGLGPSQ ASA PEEVPV ICPLSPRF
Sbjct: 121 RSDDSDFDDLIMQHLAAAASRARYVHRRERQRQFGLGPSQGASACPEEVPVLICPLSPRF 180

Query: 181 QDATLNSPNSDSPSPGSLDENEFSRTRISKISPRVLLTETSSGSQLKTNQSEGLSFPDSI 240
           QDA+L+SPNSDSPSPGS   +   RT ISKISPRVLLTETSSGSQ KTNQSEGLSFPDSI
Sbjct: 181 QDASLSSPNSDSPSPGS-PLSSGGRTGISKISPRVLLTETSSGSQQKTNQSEGLSFPDSI 240

Query: 241 KSKWSATSAKYKESIWRGTQGIKEKLLARNSSVRELSRGVKREVSAGIAGVSRMIERLDL 300
           KSKWSATSAKYKESIWR TQGIKEKLLARNSSV+ELSRGVKREVSAGIAGV+RMI+RLDL
Sbjct: 241 KSKWSATSAKYKESIWRSTQGIKEKLLARNSSVKELSRGVKREVSAGIAGVARMIDRLDL 300

Query: 301 TTKKNAASVSFFSCSGGTSNSLKGKNVV-QESAVTDGSVKIDRICTGSPSQVSPTVPAAV 360
           ++K+NAASVSFFSCSGGTSNS+KGKNVV QES VTDGSVK DRICTGSPS VSPTVP +V
Sbjct: 301 SSKRNAASVSFFSCSGGTSNSMKGKNVVMQESGVTDGSVKNDRICTGSPSHVSPTVPGSV 360

Query: 361 EVSLAQ 365
           + SLAQ
Sbjct: 361 DDSLAQ 360

[Close]
BLAST of Spg023668 vs. NCBI nr
Match: XP_038906575.1 (E3 ubiquitin-protein ligase RHF1A [Benincasa hispida])

HSP 1 Score: 560.5 bits (1443), Expect = 1.1e-155
Identity = 308/365 (84.38%), Postives = 326/365 (89.32%), Query Frame = 0 

Query: 1   MANFTFTSTSTSTPPL-LSAASDDAFEDACCICLDPFTSHDPATITSCKHEYHLQCILDW 60
           MANFT +S+S +   +  SAASDDAF+DACCICLDPFTS DPATITSCKHEYHLQCILDW
Sbjct: 1   MANFTSSSSSHNPNAVSASAASDDAFDDACCICLDPFTSDDPATITSCKHEYHLQCILDW 60

Query: 61  SQRSNECPICCQLLVLKDPVGQELLAAMASERLLKSRSLSSAASTSLRFHENFDIDHDSS 120
           SQRSNECPICCQLLVLKDPVGQELLAA+ASERLLK RSLSSAASTSLRFHENFDIDHDS+
Sbjct: 61  SQRSNECPICCQLLVLKDPVGQELLAAIASERLLKLRSLSSAASTSLRFHENFDIDHDSA 120

Query: 121 HSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQVASASPEEVPVQICPLSPRF 180
           HSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQV SA PEEV     P+SPRF
Sbjct: 121 HSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQVISACPEEV-----PMSPRF 180

Query: 181 QDATLNSPNSDSPSPGSLDENEFSRTRISKISPRVLLTETSSGSQLKTNQSEGLSFPDSI 240
           QDATL SPNSDSPSPGS   +   +T  SKIS  V+L    SGS  KTNQSEGLSFPDSI
Sbjct: 181 QDATLISPNSDSPSPGS-PLSSVGQTGTSKISHSVILL-PPSGSHQKTNQSEGLSFPDSI 240

Query: 241 KSKWSATSAKYKESIWRGTQGIKEKLLARNSSVRELSRGVKREVSAGIAGVSRMIERLDL 300
           KSKWSATSAKYKESI R TQGIKEKLLARNSS++ELSRGVKREVSAGIAGV+RMI+RLDL
Sbjct: 241 KSKWSATSAKYKESILRSTQGIKEKLLARNSSMKELSRGVKREVSAGIAGVARMIDRLDL 300

Query: 301 TTKKNAASVSFFSCSGGTSNSLKGKNVVQESAVTDGSVKIDRICTGSPSQVSPTVPAAVE 360
           T+K+N  SVSFFS SGGTSNSLKGKNVVQESAVTDGSVKIDRICTGSPSQVSPTVP +VE
Sbjct: 301 TSKRNTGSVSFFSFSGGTSNSLKGKNVVQESAVTDGSVKIDRICTGSPSQVSPTVPGSVE 358

Query: 361 VSLAQ 365
           VSLAQ
Sbjct: 361 VSLAQ 358

[Close]
BLAST of Spg023668 vs. ExPASy Swiss-Prot
Match: Q4TU14 (E3 ubiquitin-protein ligase RHF1A OS=Arabidopsis thaliana OX=3702 GN=RHF1A PE=1 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 5.4e-60
Identity = 159/370 (42.97%), Postives = 219/370 (59.19%), Query Frame = 0 

Query: 1   MANFTFTS---------------TSTSTPPLLSAASDD--AFEDACCICLDPFTSHDPAT 60
           M+NFT+TS               +S+S+   L  ASDD    +DAC ICL+PFT  DP+T
Sbjct: 1   MSNFTYTSAFNLSDNSPFNPAIGSSSSSSSALVVASDDDNNTDDACSICLEPFTLQDPST 60

Query: 61  ITSCKHEYHLQCILDWSQRSNECPICCQLLVLKDPVGQELLAAMASERLLKSRSLSSAAS 120
           +TSCKHEYHLQCI++WSQRS ECPIC QL VL+DP  QELLAA+  ERLLK+R++SS++ 
Sbjct: 61  VTSCKHEYHLQCIIEWSQRSKECPICWQLFVLRDPASQELLAAVEKERLLKTRNISSSSP 120

Query: 121 TSLRFHENFDIDHDSSHSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQVASA 180
            S+  H + D   +   S  S FD+  ++HL  AA R   + RR+ Q    +  S V+S+
Sbjct: 121 ISIH-HSHDDFHSEEEESQFSSFDEQFLRHLTEAAHRRCLLRRRDGQ----ISSSLVSSS 180

Query: 181 SPEEV-PVQICPLSPRFQDATLNSPNSD-SPSPGSLDENEFS--------RTRISKISPR 240
            P  + P  +  L      + +   NS+  PSPGS+  +  S            S+ISP 
Sbjct: 181 DPTTIHPTDLVNLYRLSAISHVEHQNSNPCPSPGSMTPSPVSGHSSIPADSNNGSRISP- 240

Query: 241 VLLTETSSGSQLKTNQSEGLSFPDSIKSKWSATSAKYKESIWRGTQGIKEKLLARNSSVR 300
                + S S       E  S P++IKSK +A SAKYKESI +  QG+KEKLLARN+SV+
Sbjct: 241 ---GPSPSRSSQSPKSPEASSLPEAIKSKLAAASAKYKESISKSKQGLKEKLLARNNSVK 300

Query: 301 ELSRGVKREVSAGIAGVSRMIERLDLTTKKNAASV---SFFSCSGGTSNSLKGKNVVQES 341
           ELS+GV+RE++AGIAGV+RMIER+D ++K+   S    +  + + G + S KGK V   S
Sbjct: 301 ELSKGVQREMNAGIAGVARMIERMDFSSKRFGGSAHVSTSTATASGFNFSFKGKRVEANS 360

[Close]
BLAST of Spg023668 vs. ExPASy Swiss-Prot
Match: Q9ZT42 (E3 ubiquitin-protein ligase RHF2A OS=Arabidopsis thaliana OX=3702 GN=RHF2A PE=2 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 2.1e-43
Identity = 141/369 (38.21%), Postives = 203/369 (55.01%), Query Frame = 0 

Query: 9   TSTSTPPLLSAAS------DDAFEDACCICLDPFTSHDPATITSCKHEYHLQCILDWSQR 68
           T+TS   L SAA+       DA +DAC ICL+ F   DP+T+TSCKHEYHLQCIL+W QR
Sbjct: 7   TTTSEGHLTSAAAFVEGGIQDACDDACSICLESFCESDPSTLTSCKHEYHLQCILEWCQR 66

Query: 69  SNECPICCQLLVLKDPVGQELLAAMASERLLKSRSLSSAASTSLRFHENFDIDHDSSHSD 128
           S++CP+C Q + LKDP  QELL A+  ER  +     +A         +F++ H     D
Sbjct: 67  SSQCPMCWQSISLKDPTSQELLEAVEQERNFRFNPTRNATIFRHPTLGDFELQHLPVGVD 126

Query: 129 DSDFDDLIMQHLAAAAS--RARYVHRRERQR---------HFGLGPSQVASASPEEVPVQ 188
           +++ ++ I+QHLAAAA+  RAR+  RRE  R          F +  SQ  ++SP   P  
Sbjct: 127 NAEIEERIIQHLAAAAAMGRARHGVRREGHRSRSSSQGHQQFMVFSSQPNASSPPPHPPM 186

Query: 189 ICPLSPRFQDATLNSPNSDSPSPGSLDENEFSRT--RISKISPRVL------LTETSSGS 248
               S R +  T+++   ++   GS   N    T     ++SP         L ++S   
Sbjct: 187 PSSPSQRDESDTVSNLPHNALGEGSHQSNTQPPTSSHPRQVSPSASDSNSRPLNQSSPSE 246

Query: 249 QLKTNQSEGLSFPDSIKSKWSATSAKYKESIWRGTQGIKEKLLARNSSVRELSRGVKREV 308
           Q +   SE  SF +S+KS+ +A S +YKESI + T+  K++L +RN+S+ +L   VKREV
Sbjct: 247 QDRAGPSELQSFSESLKSRLNAVSTRYKESISKNTRNWKDRLFSRNTSMADLGSEVKREV 306

Query: 309 SAGIAGVSRMIERLDL--TTKKNAASVSFFS--CSGGTSNSLKGKNVVQESAVTDGSVKI 349
           SAGIA VSRM+ERL+    ++ + ASVS  S   +  T+N         E +V +  VK 
Sbjct: 307 SAGIATVSRMMERLETRENSRPSTASVSDVSENHTPETNNEHNRAAAGDEHSVNERGVK- 366

[Close]
BLAST of Spg023668 vs. ExPASy Swiss-Prot
Match: Q2HIJ8 (Probable E3 ubiquitin-protein ligase RHB1A OS=Arabidopsis thaliana OX=3702 GN=RHB1A PE=2 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 1.3e-08
Identity = 24/55 (43.64%), Postives = 35/55 (63.64%), Query Frame = 0 

Query: 23  DAFEDACCICLDPFTSHDPATITSCKHEYHLQCILDWSQRSNECPICCQLLVLKD 78
           D  ED C IC + +   +P   T C+HE+HL C+L+W +RS+ CPIC + +V  D
Sbjct: 133 DEEEDCCPICFEDYDVENPRLTTKCEHEFHLSCLLEWIERSDRCPICDKEVVFDD 187

[Close]
BLAST of Spg023668 vs. ExPASy Swiss-Prot
Match: Q8LE94 (E3 ubiquitin-protein ligase At3g02290 OS=Arabidopsis thaliana OX=3702 GN=At3g02290 PE=2 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 1.3e-08
Identity = 21/48 (43.75%), Postives = 32/48 (66.67%), Query Frame = 0 

Query: 26  EDACCICLDPFTSHDPATITSCKHEYHLQCILDWSQRSNECPICCQLL 74
           ED C  CL+ +TS +P  +T C H +HL CI +W +RS  CP+C +++
Sbjct: 178 EDVCPTCLEEYTSENPKIVTKCSHHFHLSCIYEWMERSENCPVCGKVM 225

[Close]
BLAST of Spg023668 vs. ExPASy Swiss-Prot
Match: Q93ZF6 (E3 ubiquitin-protein ligase AIRP1 OS=Arabidopsis thaliana OX=3702 GN=AIRP1 PE=1 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 1.8e-07
Identity = 23/51 (45.10%), Postives = 33/51 (64.71%), Query Frame = 0 

Query: 27  DACCICLDPFTSHDPATITSCKHEYHLQCILDWSQRSNECPICCQLLVLKD 78
           D C ICL+ +   +P  +T C H++HL CIL W +RS  CP+C + LVL +
Sbjct: 102 DECPICLEEYEIDNPKLLTKCGHDFHLACILAWMERSEACPVCDKELVLTE 152

[Close]
BLAST of Spg023668 vs. ExPASy TrEMBL
Match: A0A6J1L048 (E3 ubiquitin-protein ligase RHF1A isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499166 PE=4 SV=1)

HSP 1 Score: 583.9 bits (1504), Expect = 4.6e-163
Identity = 313/366 (85.52%), Postives = 332/366 (90.71%), Query Frame = 0 

Query: 1   MANFTFTSTSTSTPPLLSAASDDAFE-DACCICLDPFTSHDPATITSCKHEYHLQCILDW 60
           MA+FT +S +  +PP     SDDAFE DACCICLDPFTSHDP +ITSCKHEYHLQCILDW
Sbjct: 1   MADFTSSSLNALSPP-----SDDAFEDDACCICLDPFTSHDPPSITSCKHEYHLQCILDW 60

Query: 61  SQRSNECPICCQLLVLKDPVGQELLAAMASERLLKSRSLSSAASTSLRFHENFDIDHDSS 120
           SQRSNECPICCQLLVL+DPVGQELLAA+ASE+LLKSRSLSS ASTSLRFHENFD+DHDSS
Sbjct: 61  SQRSNECPICCQLLVLRDPVGQELLAAIASEKLLKSRSLSSVASTSLRFHENFDVDHDSS 120

Query: 121 HSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQVASASPEEVPVQICPLSPRF 180
           HSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQ  SA PEEVPV ICPLSPRF
Sbjct: 121 HSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQGVSACPEEVPVLICPLSPRF 180

Query: 181 QDATLNSPNSDSPSPGSLDENEFSRTRISKISPRVLLTETSSGSQLKTNQSEGLSFPDSI 240
           QDATL+SPNSDSPSPGS   +   RT ISKISPRVLLTET SGSQ KTNQ EGLSFPDSI
Sbjct: 181 QDATLSSPNSDSPSPGS-PLSSGGRTGISKISPRVLLTETLSGSQQKTNQPEGLSFPDSI 240

Query: 241 KSKWSATSAKYKESIWRGTQGIKEKLLARNSSVRELSRGVKREVSAGIAGVSRMIERLDL 300
           KSKWSATSAKYKESIWR TQGIKEKLLARNSSV+ELSRGVKREVSAGIAGV+RMI+RLDL
Sbjct: 241 KSKWSATSAKYKESIWRSTQGIKEKLLARNSSVKELSRGVKREVSAGIAGVARMIDRLDL 300

Query: 301 TTKKNAASVSFFSCSGGTSNSLKGKNVV-QESAVTDGSVKIDRICTGSPSQVSPTVPAAV 360
           ++K+NAASVSFFSCSGGTSNS+KGKNVV QES VTDGSVK DRICTGSPS VSPTVP +V
Sbjct: 301 SSKRNAASVSFFSCSGGTSNSMKGKNVVMQESGVTDGSVKNDRICTGSPSHVSPTVPGSV 360

Query: 361 EVSLAQ 365
             SLAQ
Sbjct: 361 NDSLAQ 360

[Close]
BLAST of Spg023668 vs. ExPASy TrEMBL
Match: A0A6J1H5S5 (E3 ubiquitin-protein ligase RHF1A isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111460372 PE=4 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 2.3e-162
Identity = 312/366 (85.25%), Postives = 334/366 (91.26%), Query Frame = 0 

Query: 1   MANFTFTSTSTSTPPLLSAASDDAFE-DACCICLDPFTSHDPATITSCKHEYHLQCILDW 60
           MA+FT +S +  +PP     SDDAFE DACCICLDPFTSHDP +ITSCKHEYHLQCILDW
Sbjct: 1   MADFTSSSLNALSPP-----SDDAFEDDACCICLDPFTSHDPPSITSCKHEYHLQCILDW 60

Query: 61  SQRSNECPICCQLLVLKDPVGQELLAAMASERLLKSRSLSSAASTSLRFHENFDIDHDSS 120
           SQRSNECPICCQLLVL+DPVGQELLAA+A+E+LLKSRSLSS ASTSLRFHENFD+DHDSS
Sbjct: 61  SQRSNECPICCQLLVLRDPVGQELLAAIANEKLLKSRSLSSVASTSLRFHENFDVDHDSS 120

Query: 121 HSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQVASASPEEVPVQICPLSPRF 180
            SDDSDFDDLIMQHLAAAASRARYVHRRERQR FGLGPSQ ASA PEEVPV ICPLSPRF
Sbjct: 121 RSDDSDFDDLIMQHLAAAASRARYVHRRERQRQFGLGPSQGASACPEEVPVLICPLSPRF 180

Query: 181 QDATLNSPNSDSPSPGSLDENEFSRTRISKISPRVLLTETSSGSQLKTNQSEGLSFPDSI 240
           QDA+L+SPNSDSPSPGS   +   RT ISKISPRVLLTETSSGSQ KTNQSEGLSFPDSI
Sbjct: 181 QDASLSSPNSDSPSPGS-PLSSGGRTGISKISPRVLLTETSSGSQQKTNQSEGLSFPDSI 240

Query: 241 KSKWSATSAKYKESIWRGTQGIKEKLLARNSSVRELSRGVKREVSAGIAGVSRMIERLDL 300
           KSKWSATSAKYKESIWR TQGIKEKLLARNSSV+ELSRGVKREVSAGIAGV+RMI+RLDL
Sbjct: 241 KSKWSATSAKYKESIWRSTQGIKEKLLARNSSVKELSRGVKREVSAGIAGVARMIDRLDL 300

Query: 301 TTKKNAASVSFFSCSGGTSNSLKGKNVV-QESAVTDGSVKIDRICTGSPSQVSPTVPAAV 360
           ++K+NAASVSFFSCSGGTSNS+KGKNVV QES VTDGSVK DRICTGSPS VSPTVP +V
Sbjct: 301 SSKRNAASVSFFSCSGGTSNSMKGKNVVMQESGVTDGSVKNDRICTGSPSHVSPTVPGSV 360

Query: 361 EVSLAQ 365
           + SLAQ
Sbjct: 361 DDSLAQ 360

[Close]
BLAST of Spg023668 vs. ExPASy TrEMBL
Match: A0A0A0KDQ6 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G153470 PE=4 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 2.5e-153
Identity = 302/369 (81.84%), Postives = 322/369 (87.26%), Query Frame = 0 

Query: 1   MANFTFTSTSTSTPP----LLSAASDDAFEDACCICLDPFTSHDPATITSCKHEYHLQCI 60
           MANFT +S+  S  P      +AASDD F+D CCICLDPFTS DPATITSCKHEYHLQCI
Sbjct: 1   MANFTSSSSFVSLNPNAVSASAAASDDDFDDVCCICLDPFTSDDPATITSCKHEYHLQCI 60

Query: 61  LDWSQRSNECPICCQLLVLKDPVGQELLAAMASERLLKSRSLSSAASTSLRFHENFDIDH 120
           LDWSQRS+ECPICCQLLVLKDPVGQELLAAMASERLLKSR LSSAASTSL FHENFD DH
Sbjct: 61  LDWSQRSDECPICCQLLVLKDPVGQELLAAMASERLLKSRGLSSAASTSLHFHENFDFDH 120

Query: 121 DSSHSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQVASASPEEVPVQICPLS 180
           DS HSDDSDFDDLIMQHLAAAASRARYV RRERQRHFG+GPSQV+SA PE       P+S
Sbjct: 121 DSVHSDDSDFDDLIMQHLAAAASRARYVQRRERQRHFGVGPSQVSSACPE------VPMS 180

Query: 181 PRFQDATLNSPNSDSPSPGSLDENEFSRTRISKISPRV-LLTETSSGSQLKTNQSEGLSF 240
           PRFQDATL SPNSDSPSP S       RT I+KISP V LL +T SGSQ KTNQSEGLSF
Sbjct: 181 PRFQDATLTSPNSDSPSPSS-PMPSVGRTGINKISPSVILLPDTPSGSQQKTNQSEGLSF 240

Query: 241 PDSIKSKWSATSAKYKESIWRGTQGIKEKLLARNSSVRELSRGVKREVSAGIAGVSRMIE 300
            DSIKSKWSATSAKYKESI R TQGIKEKLLARNSSV+ELS+GVKREVSAGIAGV+RMI+
Sbjct: 241 QDSIKSKWSATSAKYKESILRSTQGIKEKLLARNSSVKELSKGVKREVSAGIAGVARMID 300

Query: 301 RLDLTTKKNAASVSFFSCSGGTSNSLKGKNVVQESAVTDGSVKIDRICTGSPSQVSPTVP 360
           RLDLT+K+N  SVSFFSCSGGTSNSLKGKNVVQESAVTDGSVKI+RICTGSPSQVSPTVP
Sbjct: 301 RLDLTSKRNTGSVSFFSCSGGTSNSLKGKNVVQESAVTDGSVKINRICTGSPSQVSPTVP 360

Query: 361 AAVEVSLAQ 365
           ++V+VSLAQ
Sbjct: 361 SSVKVSLAQ 362

[Close]
BLAST of Spg023668 vs. ExPASy TrEMBL
Match: A0A1S3C9D8 (E3 ubiquitin-protein ligase RHF1A OS=Cucumis melo OX=3656 GN=LOC103497932 PE=4 SV=1)

HSP 1 Score: 548.5 bits (1412), Expect = 2.2e-152
Identity = 306/375 (81.60%), Postives = 324/375 (86.40%), Query Frame = 0 

Query: 1   MANFTFTS----TSTSTPP------LLSAASDDAFEDACCICLDPFTSHDPATITSCKHE 60
           MANFT +S    +S S  P        +AASDD F+DACCICLDPFTS DPATITSCKHE
Sbjct: 1   MANFTSSSSPSPSSVSLNPNAVSASAAAAASDDDFDDACCICLDPFTSDDPATITSCKHE 60

Query: 61  YHLQCILDWSQRSNECPICCQLLVLKDPVGQELLAAMASERLLKSRSLSSAASTSLRFHE 120
           YHLQCILDWSQRS+ECPICCQLL LKDPVGQELLAAMASERLLKSRSLSSAASTSLRFHE
Sbjct: 61  YHLQCILDWSQRSDECPICCQLLALKDPVGQELLAAMASERLLKSRSLSSAASTSLRFHE 120

Query: 121 NFDIDHDSSHSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQVASASPEEVPV 180
           NFD DHDS HSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFG+GPSQVASA PE    
Sbjct: 121 NFDFDHDSVHSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGVGPSQVASACPE---- 180

Query: 181 QICPLSPRFQDATLNSPNSDSPSPGSLDENEFSRTRISKISPRV-LLTETSSGSQLKTNQ 240
              P+SPRFQDATL SPNSDSPSPGS       +T I+KISP V LL +T SGSQ KTNQ
Sbjct: 181 --VPMSPRFQDATLTSPNSDSPSPGS-PMPSVGQTGINKISPSVILLPDTPSGSQQKTNQ 240

Query: 241 SEGLSFPDSIKSKWSATSAKYKESIWRGTQGIKEKLLARNSSVRELSRGVKREVSAGIAG 300
           SEGLSF DSIKSKWSATSAKYKESI R TQGIKEKLLARNSSV+ELS+GVKREVSAGIAG
Sbjct: 241 SEGLSFQDSIKSKWSATSAKYKESILRSTQGIKEKLLARNSSVKELSKGVKREVSAGIAG 300

Query: 301 VSRMIERLDLTTKKNAASVSFFSCSGGTSNSLKGKNVVQESAVTDGSVKIDRICTGSPSQ 360
           V+RMI+RLDLT+K+N  SVSFFSC GGTSNSLKGKNVVQESA TDGSVKI+RICTGSPSQ
Sbjct: 301 VARMIDRLDLTSKRNTGSVSFFSC-GGTSNSLKGKNVVQESAGTDGSVKINRICTGSPSQ 360

Query: 361 VSPTVPAAVEVSLAQ 365
           VSPTVP +VEVSLAQ
Sbjct: 361 VSPTVPGSVEVSLAQ 367

[Close]
BLAST of Spg023668 vs. ExPASy TrEMBL
Match: A0A6J1H7X0 (E3 ubiquitin-protein ligase RHF1A isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111460372 PE=4 SV=1)

HSP 1 Score: 544.7 bits (1402), Expect = 3.1e-151
Identity = 297/366 (81.15%), Postives = 319/366 (87.16%), Query Frame = 0 

Query: 1   MANFTFTSTSTSTPPLLSAASDDAFE-DACCICLDPFTSHDPATITSCKHEYHLQCILDW 60
           MA+FT +S +  +PP     SDDAFE DACCICLDPFTSHDP +ITSCKHEYHLQCILDW
Sbjct: 1   MADFTSSSLNALSPP-----SDDAFEDDACCICLDPFTSHDPPSITSCKHEYHLQCILDW 60

Query: 61  SQRSNECPICCQLLVLKDPVGQELLAAMASERLLKSRSLSSAASTSLRFHENFDIDHDSS 120
           SQRSNECPICCQLLVL+DPVGQELLAA+A+E+LLKSRSLSS ASTSLRFHENFD+DH   
Sbjct: 61  SQRSNECPICCQLLVLRDPVGQELLAAIANEKLLKSRSLSSVASTSLRFHENFDVDH--- 120

Query: 121 HSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQVASASPEEVPVQICPLSPRF 180
                        HLAAAASRARYVHRRERQR FGLGPSQ ASA PEEVPV ICPLSPRF
Sbjct: 121 -------------HLAAAASRARYVHRRERQRQFGLGPSQGASACPEEVPVLICPLSPRF 180

Query: 181 QDATLNSPNSDSPSPGSLDENEFSRTRISKISPRVLLTETSSGSQLKTNQSEGLSFPDSI 240
           QDA+L+SPNSDSPSPGS   +   RT ISKISPRVLLTETSSGSQ KTNQSEGLSFPDSI
Sbjct: 181 QDASLSSPNSDSPSPGS-PLSSGGRTGISKISPRVLLTETSSGSQQKTNQSEGLSFPDSI 240

Query: 241 KSKWSATSAKYKESIWRGTQGIKEKLLARNSSVRELSRGVKREVSAGIAGVSRMIERLDL 300
           KSKWSATSAKYKESIWR TQGIKEKLLARNSSV+ELSRGVKREVSAGIAGV+RMI+RLDL
Sbjct: 241 KSKWSATSAKYKESIWRSTQGIKEKLLARNSSVKELSRGVKREVSAGIAGVARMIDRLDL 300

Query: 301 TTKKNAASVSFFSCSGGTSNSLKGKNVV-QESAVTDGSVKIDRICTGSPSQVSPTVPAAV 360
           ++K+NAASVSFFSCSGGTSNS+KGKNVV QES VTDGSVK DRICTGSPS VSPTVP +V
Sbjct: 301 SSKRNAASVSFFSCSGGTSNSMKGKNVVMQESGVTDGSVKNDRICTGSPSHVSPTVPGSV 344

Query: 361 EVSLAQ 365
           + SLAQ
Sbjct: 361 DDSLAQ 344

[Close]
BLAST of Spg023668 vs. TAIR 10
Match: AT4G14220.1 (RING-H2 group F1A )

HSP 1 Score: 233.0 bits (593), Expect = 3.9e-61
Identity = 159/370 (42.97%), Postives = 219/370 (59.19%), Query Frame = 0 

Query: 1   MANFTFTS---------------TSTSTPPLLSAASDD--AFEDACCICLDPFTSHDPAT 60
           M+NFT+TS               +S+S+   L  ASDD    +DAC ICL+PFT  DP+T
Sbjct: 1   MSNFTYTSAFNLSDNSPFNPAIGSSSSSSSALVVASDDDNNTDDACSICLEPFTLQDPST 60

Query: 61  ITSCKHEYHLQCILDWSQRSNECPICCQLLVLKDPVGQELLAAMASERLLKSRSLSSAAS 120
           +TSCKHEYHLQCI++WSQRS ECPIC QL VL+DP  QELLAA+  ERLLK+R++SS++ 
Sbjct: 61  VTSCKHEYHLQCIIEWSQRSKECPICWQLFVLRDPASQELLAAVEKERLLKTRNISSSSP 120

Query: 121 TSLRFHENFDIDHDSSHSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQVASA 180
            S+  H + D   +   S  S FD+  ++HL  AA R   + RR+ Q    +  S V+S+
Sbjct: 121 ISIH-HSHDDFHSEEEESQFSSFDEQFLRHLTEAAHRRCLLRRRDGQ----ISSSLVSSS 180

Query: 181 SPEEV-PVQICPLSPRFQDATLNSPNSD-SPSPGSLDENEFS--------RTRISKISPR 240
            P  + P  +  L      + +   NS+  PSPGS+  +  S            S+ISP 
Sbjct: 181 DPTTIHPTDLVNLYRLSAISHVEHQNSNPCPSPGSMTPSPVSGHSSIPADSNNGSRISP- 240

Query: 241 VLLTETSSGSQLKTNQSEGLSFPDSIKSKWSATSAKYKESIWRGTQGIKEKLLARNSSVR 300
                + S S       E  S P++IKSK +A SAKYKESI +  QG+KEKLLARN+SV+
Sbjct: 241 ---GPSPSRSSQSPKSPEASSLPEAIKSKLAAASAKYKESISKSKQGLKEKLLARNNSVK 300

Query: 301 ELSRGVKREVSAGIAGVSRMIERLDLTTKKNAASV---SFFSCSGGTSNSLKGKNVVQES 341
           ELS+GV+RE++AGIAGV+RMIER+D ++K+   S    +  + + G + S KGK V   S
Sbjct: 301 ELSKGVQREMNAGIAGVARMIERMDFSSKRFGGSAHVSTSTATASGFNFSFKGKRVEANS 360

[Close]
BLAST of Spg023668 vs. TAIR 10
Match: AT5G22000.2 (RING-H2 group F2A )

HSP 1 Score: 179.1 bits (453), Expect = 6.6e-45
Identity = 142/370 (38.38%), Postives = 204/370 (55.14%), Query Frame = 0 

Query: 8   STSTSTPPLLSAAS------DDAFEDACCICLDPFTSHDPATITSCKHEYHLQCILDWSQ 67
           ST+TS   L SAA+       DA +DAC ICL+ F   DP+T+TSCKHEYHLQCIL+W Q
Sbjct: 6   STTTSEGHLTSAAAFVEGGIQDACDDACSICLESFCESDPSTLTSCKHEYHLQCILEWCQ 65

Query: 68  RSNECPICCQLLVLKDPVGQELLAAMASERLLKSRSLSSAASTSLRFHENFDIDHDSSHS 127
           RS++CP+C Q + LKDP  QELL A+  ER  +     +A         +F++ H     
Sbjct: 66  RSSQCPMCWQSISLKDPTSQELLEAVEQERNFRFNPTRNATIFRHPTLGDFELQHLPVGV 125

Query: 128 DDSDFDDLIMQHLAAAAS--RARYVHRRERQR---------HFGLGPSQVASASPEEVPV 187
           D+++ ++ I+QHLAAAA+  RAR+  RRE  R          F +  SQ  ++SP   P 
Sbjct: 126 DNAEIEERIIQHLAAAAAMGRARHGVRREGHRSRSSSQGHQQFMVFSSQPNASSPPPHPP 185

Query: 188 QICPLSPRFQDATLNSPNSDSPSPGSLDENEFSRT--RISKISPRVL------LTETSSG 247
                S R +  T+++   ++   GS   N    T     ++SP         L ++S  
Sbjct: 186 MPSSPSQRDESDTVSNLPHNALGEGSHQSNTQPPTSSHPRQVSPSASDSNSRPLNQSSPS 245

Query: 248 SQLKTNQSEGLSFPDSIKSKWSATSAKYKESIWRGTQGIKEKLLARNSSVRELSRGVKRE 307
            Q +   SE  SF +S+KS+ +A S +YKESI + T+  K++L +RN+S+ +L   VKRE
Sbjct: 246 EQDRAGPSELQSFSESLKSRLNAVSTRYKESISKNTRNWKDRLFSRNTSMADLGSEVKRE 305

Query: 308 VSAGIAGVSRMIERLDL--TTKKNAASVSFFS--CSGGTSNSLKGKNVVQESAVTDGSVK 349
           VSAGIA VSRM+ERL+    ++ + ASVS  S   +  T+N         E +V +  VK
Sbjct: 306 VSAGIATVSRMMERLETRENSRPSTASVSDVSENHTPETNNEHNRAAAGDEHSVNERGVK 365

[Close]
BLAST of Spg023668 vs. TAIR 10
Match: AT5G22000.1 (RING-H2 group F2A )

HSP 1 Score: 177.9 bits (450), Expect = 1.5e-44
Identity = 141/369 (38.21%), Postives = 203/369 (55.01%), Query Frame = 0 

Query: 9   TSTSTPPLLSAAS------DDAFEDACCICLDPFTSHDPATITSCKHEYHLQCILDWSQR 68
           T+TS   L SAA+       DA +DAC ICL+ F   DP+T+TSCKHEYHLQCIL+W QR
Sbjct: 7   TTTSEGHLTSAAAFVEGGIQDACDDACSICLESFCESDPSTLTSCKHEYHLQCILEWCQR 66

Query: 69  SNECPICCQLLVLKDPVGQELLAAMASERLLKSRSLSSAASTSLRFHENFDIDHDSSHSD 128
           S++CP+C Q + LKDP  QELL A+  ER  +     +A         +F++ H     D
Sbjct: 67  SSQCPMCWQSISLKDPTSQELLEAVEQERNFRFNPTRNATIFRHPTLGDFELQHLPVGVD 126

Query: 129 DSDFDDLIMQHLAAAAS--RARYVHRRERQR---------HFGLGPSQVASASPEEVPVQ 188
           +++ ++ I+QHLAAAA+  RAR+  RRE  R          F +  SQ  ++SP   P  
Sbjct: 127 NAEIEERIIQHLAAAAAMGRARHGVRREGHRSRSSSQGHQQFMVFSSQPNASSPPPHPPM 186

Query: 189 ICPLSPRFQDATLNSPNSDSPSPGSLDENEFSRT--RISKISPRVL------LTETSSGS 248
               S R +  T+++   ++   GS   N    T     ++SP         L ++S   
Sbjct: 187 PSSPSQRDESDTVSNLPHNALGEGSHQSNTQPPTSSHPRQVSPSASDSNSRPLNQSSPSE 246

Query: 249 QLKTNQSEGLSFPDSIKSKWSATSAKYKESIWRGTQGIKEKLLARNSSVRELSRGVKREV 308
           Q +   SE  SF +S+KS+ +A S +YKESI + T+  K++L +RN+S+ +L   VKREV
Sbjct: 247 QDRAGPSELQSFSESLKSRLNAVSTRYKESISKNTRNWKDRLFSRNTSMADLGSEVKREV 306

Query: 309 SAGIAGVSRMIERLDL--TTKKNAASVSFFS--CSGGTSNSLKGKNVVQESAVTDGSVKI 349
           SAGIA VSRM+ERL+    ++ + ASVS  S   +  T+N         E +V +  VK 
Sbjct: 307 SAGIATVSRMMERLETRENSRPSTASVSDVSENHTPETNNEHNRAAAGDEHSVNERGVK- 366

[Close]
BLAST of Spg023668 vs. TAIR 10
Match: AT5G22000.3 (RING-H2 group F2A )

HSP 1 Score: 177.9 bits (450), Expect = 1.5e-44
Identity = 141/369 (38.21%), Postives = 203/369 (55.01%), Query Frame = 0 

Query: 9   TSTSTPPLLSAAS------DDAFEDACCICLDPFTSHDPATITSCKHEYHLQCILDWSQR 68
           T+TS   L SAA+       DA +DAC ICL+ F   DP+T+TSCKHEYHLQCIL+W QR
Sbjct: 7   TTTSEGHLTSAAAFVEGGIQDACDDACSICLESFCESDPSTLTSCKHEYHLQCILEWCQR 66

Query: 69  SNECPICCQLLVLKDPVGQELLAAMASERLLKSRSLSSAASTSLRFHENFDIDHDSSHSD 128
           S++CP+C Q + LKDP  QELL A+  ER  +     +A         +F++ H     D
Sbjct: 67  SSQCPMCWQSISLKDPTSQELLEAVEQERNFRFNPTRNATIFRHPTLGDFELQHLPVGVD 126

Query: 129 DSDFDDLIMQHLAAAAS--RARYVHRRERQR---------HFGLGPSQVASASPEEVPVQ 188
           +++ ++ I+QHLAAAA+  RAR+  RRE  R          F +  SQ  ++SP   P  
Sbjct: 127 NAEIEERIIQHLAAAAAMGRARHGVRREGHRSRSSSQGHQQFMVFSSQPNASSPPPHPPM 186

Query: 189 ICPLSPRFQDATLNSPNSDSPSPGSLDENEFSRT--RISKISPRVL------LTETSSGS 248
               S R +  T+++   ++   GS   N    T     ++SP         L ++S   
Sbjct: 187 PSSPSQRDESDTVSNLPHNALGEGSHQSNTQPPTSSHPRQVSPSASDSNSRPLNQSSPSE 246

Query: 249 QLKTNQSEGLSFPDSIKSKWSATSAKYKESIWRGTQGIKEKLLARNSSVRELSRGVKREV 308
           Q +   SE  SF +S+KS+ +A S +YKESI + T+  K++L +RN+S+ +L   VKREV
Sbjct: 247 QDRAGPSELQSFSESLKSRLNAVSTRYKESISKNTRNWKDRLFSRNTSMADLGSEVKREV 306

Query: 309 SAGIAGVSRMIERLDL--TTKKNAASVSFFS--CSGGTSNSLKGKNVVQESAVTDGSVKI 349
           SAGIA VSRM+ERL+    ++ + ASVS  S   +  T+N         E +V +  VK 
Sbjct: 307 SAGIATVSRMMERLETRENSRPSTASVSDVSENHTPETNNEHNRAAAGDEHSVNERGVK- 366

[Close]
BLAST of Spg023668 vs. TAIR 10
Match: AT3G02290.1 (RING/U-box superfamily protein )

HSP 1 Score: 62.4 bits (150), Expect = 9.0e-10
Identity = 21/48 (43.75%), Postives = 32/48 (66.67%), Query Frame = 0 

Query: 26  EDACCICLDPFTSHDPATITSCKHEYHLQCILDWSQRSNECPICCQLL 74
           ED C  CL+ +TS +P  +T C H +HL CI +W +RS  CP+C +++
Sbjct: 178 EDVCPTCLEEYTSENPKIVTKCSHHFHLSCIYEWMERSENCPVCGKVM 225

The following BLAST results are available for this feature:
BLAST of Spg023668 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_023549293.17.3e-16385.52E3 ubiquitin-protein ligase RHF1A isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_023006440.19.6e-16385.52E3 ubiquitin-protein ligase RHF1A isoform X1 [Cucurbita maxima][more]
XP_022959383.14.7e-16285.25E3 ubiquitin-protein ligase RHF1A isoform X1 [Cucurbita moschata][more]
KAG6575008.11.8e-16184.97E3 ubiquitin-protein ligase RHF1A, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_038906575.11.1e-15584.38E3 ubiquitin-protein ligase RHF1A [Benincasa hispida][more]
BLAST of Spg023668 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q4TU145.4e-6042.97E3 ubiquitin-protein ligase RHF1A OS=Arabidopsis thaliana OX=3702 GN=RHF1A PE=1 ... [more]
Q9ZT422.1e-4338.21E3 ubiquitin-protein ligase RHF2A OS=Arabidopsis thaliana OX=3702 GN=RHF2A PE=2 ... [more]
Q2HIJ81.3e-0843.64Probable E3 ubiquitin-protein ligase RHB1A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
Q8LE941.3e-0843.75E3 ubiquitin-protein ligase At3g02290 OS=Arabidopsis thaliana OX=3702 GN=At3g022... [more]
Q93ZF61.8e-0745.10E3 ubiquitin-protein ligase AIRP1 OS=Arabidopsis thaliana OX=3702 GN=AIRP1 PE=1 ... [more]
BLAST of Spg023668 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1L0484.6e-16385.52E3 ubiquitin-protein ligase RHF1A isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1H5S52.3e-16285.25E3 ubiquitin-protein ligase RHF1A isoform X1 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A0A0KDQ62.5e-15381.84RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G153470 P... [more]
A0A1S3C9D82.2e-15281.60E3 ubiquitin-protein ligase RHF1A OS=Cucumis melo OX=3656 GN=LOC103497932 PE=4 S... [more]
A0A6J1H7X03.1e-15181.15E3 ubiquitin-protein ligase RHF1A isoform X2 OS=Cucurbita moschata OX=3662 GN=LO... [more]
BLAST of Spg023668 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G14220.13.9e-6142.97RING-H2 group F1A [more]
AT5G22000.26.6e-4538.38RING-H2 group F2A [more]
AT5G22000.11.5e-4438.21RING-H2 group F2A [more]
AT5G22000.31.5e-4438.21RING-H2 group F2A [more]
AT3G02290.19.0e-1043.75RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 29..69
e-value: 1.7E-6
score: 37.6
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 28..69
e-value: 1.0E-8
score: 35.4
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 29..69
score: 11.854899
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 12..101
e-value: 3.1E-13
score: 50.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 179..203
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 218..237
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 179..201
NoneNo IPR availablePANTHERPTHR46463ZINC FINGER, RING/FYVE/PHD-TYPEcoord: 2..340
NoneNo IPR availablePANTHERPTHR46463:SF16E3 UBIQUITIN-PROTEIN LIGASE RHF1Acoord: 2..340
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 23..76

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg023668.1Spg023668.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009561 megagametogenesis
biological_process GO:0055046 microgametogenesis
biological_process GO:0010498 proteasomal protein catabolic process
biological_process GO:0016567 protein ubiquitination
biological_process GO:0051726 regulation of cell cycle
molecular_function GO:0004842 ubiquitin-protein transferase activity